Parasites, Hosts and Diseases

"Somchai Pinlaor"

Original Articles

Establishment of an Allo-Transplantable Hamster Cholangiocarcinoma Cell Line and Its Application for In Vivo Screening of Anti-Cancer Drugs: Nattapong Puthdee, Kulthida Vaeteewoottacharn, Wunchana Seubwai, Orasa Wonkchalee, Worasak Kaewkong, Amornrat Juasook, Somchai Pinlaor, Chawalit Pairojkul, Chaisiri Wongkham, Seiji Okada, Thidarut Boonmars, Sopit Wongkham; Korean J Parasitol 2013;51(6):711-717.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.711

Abstract
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Opisthorchis viverrini (O. viverrini) is a well-known causative agent of cholangiocarcinoma (CCA) in humans. CCA is very resistant to chemotherapy and is frequently fatal. To understand the pathogenesis of CCA in humans, a rodent model was developed. However, the development of CCA in rodents is time-consuming and the xenograft-transplantation model of human CCA in immunodeficient mice is costly. Therefore, the establishment of an in vivo screening model for O. viverrini-associated CCA treatment was of interest. We developed a hamster CCA cell line, Ham-1, derived from the CCA tissue of O. viverrini-infected and N-nitrosodimethylamine-treated Syrian golden hamsters. Ham-1 has been maintained in Dulbecco's Modified Essential Medium supplemented with 10% fetal bovine serum for more than 30 subcultures. These cells are mostly diploid (2n=44) with some being polyploid. Tumorigenic properties of Ham-1 were demonstrated by allograft transplantation in hamsters. The transplanted tissues were highly proliferative and exhibited a glandular-like structure retaining a bile duct marker, cytokeratin 19. The usefulness of this for in vivo model was demonstrated by berberine treatment, a traditional medicine that is active against various cancers. Growth inhibitory effects of berberine, mainly by an induction of G1 cell cycle arrest, were observed in vitro and in vivo. In summary, we developed the allo-transplantable hamster CCA cell line, which can be used for chemotherapeutic drug testing in vitro and in vivo.

Citations

Citations to this article as recorded by

The Role of Herbal Medicine in Cholangiocarcinoma Control: A Systematic Review
Kesara Na-Bangchang, Tullayakorn Plengsuriyakarn, Juntra Karbwang
Planta Medica.2023; 89(01): 3. CrossRef
Repurposing of plant alkaloids for cancer therapy: Pharmacology and toxicology
Thomas Efferth, Franz Oesch
Seminars in Cancer Biology.2021; 68: 143. CrossRef
A tumorigenic cell line derived from a hamster cholangiocarcinoma associated with Opisthorchis felineus liver fluke infection
Viatcheslav A. Mordvinov, Galina A. Minkova, Anna V. Kovner, Dmitriy V. Ponomarev, Maria N. Lvova, Oxana Zaparina, Svetlana A. Romanenko, Alexander G. Shilov, Maria Y. Pakharukova
Life Sciences.2021; 277: 119494. CrossRef
Mucin-producing hamster cholangiocarcinoma cell line, Ham-2, possesses the aggressive cancer phenotypes with liver and lung metastases
Piyanard Boonnate, Kulthida Vaeteewoottacharn, Ryusho Kariya, Sawako Fujikawa, Thidarut Boonmars, Somchai Pinlaor, Chawalit Pairojkul, Seiji Okada
In Vitro Cellular & Developmental Biology - Animal.2021; 57(8): 825. CrossRef
Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived Xenograft Mouse Model
Kulthida Vaeteewoottacharn, Chawalit Pairojkul, Ryusho Kariya, Kanha Muisuk, Kanokwan Imtawil, Yaovalux Chamgramol, Vajarabhongsa Bhudhisawasdi, Narong Khuntikeo, Ake Pugkhem, O-Tur Saeseow, Atit Silsirivanit, Chaisiri Wongkham, Sopit Wongkham, Seiji Okad
Cells.2019; 8(5): 496. CrossRef
Anticancer effect of berberine based on experimental animal models of various cancers: a systematic review and meta-analysis
Jianhao Xu, Yuming Long, Liwei Ni, Xuya Yuan, Na Yu, Runhong Wu, Jialong Tao, Yusong Zhang
BMC Cancer.2019;[Epub] CrossRef
Preventive and Therapeutic Roles of Berberine in Gastrointestinal Cancers
Siwang Hu, Ruochi Zhao, Yahui Liu, Junzheng Chen, Zhijian Zheng, Shuangshuang Wang
BioMed Research International.2019; 2019: 1. CrossRef
Nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in Opisthorchis viverrini-infected hamsters
Lakhanawan Charoensuk, Porntip Pinlaor, Supason Wanichwecharungruang, Kitti Intuyod, Kulthida Vaeteewoottacharn, Apisit Chaidee, Puangrat Yongvanit, Chawalit Pairojkul, Natthakitta Suwannateep, Somchai Pinlaor
Nanomedicine: Nanotechnology, Biology and Medicine.2016; 12(1): 21. CrossRef
Antitumor effect of forbesione isolated from Garcinia hanburyi on cholangiocarcinoma in vitro and in vivo
Parichart Boueroy, Chariya Hahnvajanawong, Thidarut Boonmars, Sunitta Saensa-Ard, Natthinee Anantachoke, Kulthida Vaeteewoottacharn, Vichai Reutrakul
Oncology Letters.2016; 12(6): 4685. CrossRef

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Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand: Somchai Pinlaor, Sudarat Onsurathum, Thidarut Boonmars, Porntip Pinlaor, Nuttanan Hongsrichan, Apisit Chaidee, Ornuma Haonon, Wutipong Limviroj, Smarn Tesana, Sasithorn Kaewkes, Paiboon Sithithaworn; Korean J Parasitol 2013;51(6):703-710.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.703

Abstract
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To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of Opisthorchis viverrini metacercariae (OV MC) was investigated in freshwater fish obtained from 20 provinces in northeastern Thailand between April 2011 and February 2012. A cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. Fish, were collected in each of the rainy and winter seasons from each province. Fish were identified, counted, weighed, and digested using pepsin-HCl. Samples were examined for OV MC by a sedimentation method, and metacercariae were identified under a stereomicroscope. OV MC were found in 6 species of fish; i.e., Cyclocheilichthys armatus, Puntius orphoides, Hampala dispar, Henicorhynchus siamensis, Osteochilus hasselti, and Puntioplites proctozysron from localities in 13 provinces. Among the sites where OV MC-infected fish were found, 70.0% were dams, 23.7% were ponds/lakes, and 7.7% were rivers. The mean intensity of OV MC ranged from 0.01 to 6.5 cysts per fish (or 1.3-287.5 cysts per kg of fish). A high mean intensity of OV MC per fish (>3 cysts) was found in 5 provinces: Amnat Charoen (6.5 cysts), Nakhon Phanom (4.3), Mukdahan (4.1), Khon Kaen, (3.5) and Si Sa Ket (3.4). In conclusion, OV MC are prevalent in natural cyprinid fish, with the infection rate varying according to fish species and habitats.

Citations

Citations to this article as recorded by

High-fat/high-fructose diet and Opisthorchis viverrini infection promote metabolic dysfunction-associated steatotic liver disease via inflammation, fibrogenesis, and metabolic dysfunction
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Acta Tropica.2025; 261: 107491. CrossRef
Development of a Dielectric Heating System for Selective Thermal Targeting of Liver Fluke Regions in Cirrhinus microlepis
Supatinee Kornsing, Sirigiet Phunklang, Chanchai Thongsopa, Piyaporn Krachodnok, Nuchanart Santalunai, Samran Santalunai
Applied Sciences.2025; 15(10): 5466. CrossRef
Distribution of Opisthorchis viverrini metacercariae and cyprinoid fish abundance in Nong Han Wetland, Thailand
Wiwat Kaensa, Lakhanawan Charoensuk, Chadon Nakmai, Somchai Pinlaor, Apiporn T. Suwannatrai, Umawadee Laothong, Suksanti Prakobwong
Parasite Epidemiology and Control.2025; 30: e00447. CrossRef
Unpacking the black box: I. Knowledge production and public health narratives on liver fluke infection and cholangiocarcinoma in Northeast Thailand
Yee Ting Lee, Alan D. Ziegler
South East Asia Research.2025; 33(2): 133. CrossRef
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The Journal of Infectious Diseases.2025;[Epub] CrossRef
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Yi-Chen Wang, Andrea Law, Jutamas Namsanor, Paiboon Sithithaworn, Narong Khuntikeo
Annals of the American Association of Geographers.2024; 114(8): 1781. CrossRef
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Sandy Kha, Sujittra Chaiyadet, Prasert Saichua, Sirikachorn Tangkawatana, Banchob Sripa, Sutas Suttiprapa
Parasitology Research.2024;[Epub] CrossRef
Hight prevalent of Opisthorchis viverrini infection and coincident Haplorchis metacercariae in cyprinid fishes in upper northeastern region of Thailand
Naiyana Senasri, Nattiya Chumnanka, Patcharawalai Sriyasak, Supannee Suwanpakdee, Sugunya Kumla, Kosit Sreeputhorn
Parasite Epidemiology and Control.2024; 25: e00348. CrossRef
Exploring the second intermediate hosts and morphology of human- and cat-specific Opisthorchis viverrini-like populations
Vania Agustina, Prasert Saichua, Thewarach Laha, Sirikachorn Tangkawatana, Suksanti Prakobwong, Nonglak Laoprom, Wanrak Kamphasri, Chonteera Chareonchai, David Blair, Sutas Suttiprapa
International Journal for Parasitology.2024; 54(10): 497. CrossRef
Integrated One-Health approach for prevention and control of Opisthorchis viverrini infection in rural Thailand: a 3-year study
Lakhanawan Charoensuk, Kacha Chedtabud, Suwit Chaipibool, Umawadee Laothong, Apiporn Suwannatrai, Somchai Pinlaor, Suksanti Prakobwong
Parasitology Research.2024;[Epub] CrossRef
Spatial Predictive Modeling of Liver Fluke Opisthorchis viverrine (OV) Infection under the Mathematical Models in Hexagonal Symmetrical Shapes Using Machine Learning-Based Forest Classification Regression
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Narueset Prasertsri, Phusit Khamphilung, Satith Sangpradid, Nutchanat Buasri, Theeraya Uttha, Donald Slack
Symmetry.2024; 16(8): 1067. CrossRef
Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters
Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
Parasite.2024; 31: 54. CrossRef
Spatial prediction of the probability of liver fluke infection in water resource within sub-basin using an optimized geographically-weighted regression model
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Nutchanat Buasri, Donald Slack
Frontiers in Veterinary Science.2024;[Epub] CrossRef
Temperature and Preparation Time Intervals on Survival of Opisthorchis viverrini Metacercariae in Pickled Fish (Pla‐som)
Naiyana Senasri, Nattiya Chumnanka, Wiboonsuk Talkul, Zheng-Fei Yan
International Journal of Food Science.2024;[Epub] CrossRef
Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
EFSA Journal.2024;[Epub] CrossRef
A study protocol for a multi-country cluster randomized controlled trial of the impact of a multi-component One Health strategy to eliminate Opisthorchis viverrini and soil transmitted helminths in the Lower Mekong Basin
Mary Lorraine Mationg, Archie C. A. Clements, Gail M. Williams, Matthew Kelly, Donald E. Stewart, Catherine A. Gordon, Kinley Wangdi, Sirikachorn Tangkawattana, Apiporn T. Suwannatrai, Vanathom Savathdy, Visal Khieu, Sangduan Wannachart, Suji Yoo O’Connor
Trials.2024;[Epub] CrossRef
Examining ecosystem (dis-)services on liver fluke infection in rural Northeast Thailand
Yi-Chen Wang, Andrea Law, Jutamas Namsanor, Paiboon Sithithaworn
Infectious Diseases of Poverty.2023;[Epub] CrossRef
Modelling morbidity for neglected tropical diseases: the long and winding road from cumulative exposure to long-term pathology
Anna Borlase, Joaquin M. Prada, Thomas Crellen
Philosophical Transactions of the Royal Society B: Biological Sciences.2023;[Epub] CrossRef
Machine-Learning-Based Forest Classification and Regression (FCR) for Spatial Prediction of Liver Fluke Opisthorchis viverrini (OV) Infection in Small Sub-Watersheds
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Kanokwan Bootyothee, Atchara Artchayasawat, Phusit Khamphilung, Donald Slack
ISPRS International Journal of Geo-Information.2023; 12(12): 503. CrossRef
Infection rate of Opisthorchis viverrini metacercariae in cyprinoid fish from the markets and its association to human opisthorchiasis in the local community in the Northeast Thailand
Lakhanawan Charoensuk, Alexis Ribas, Kacha Chedtabud, Suksanti Prakobwong
Acta Tropica.2022; 225: 106216. CrossRef
High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
Parasitology.2022; 149(7): 913. CrossRef
Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini
Sujittra Chaiyadet, Sirikachorn Tangkawattana, Michael J. Smout, Wannaporn Ittiprasert, Victoria H. Mann, Raksawan Deenonpoe, Patpicha Arunsan, Alex Loukas, Paul J. Brindley, Thewarach Laha, Kenneth D. Vernick
PLOS Pathogens.2022; 18(9): e1010839. CrossRef
Curcumin-loaded nanocomplexes ameliorate the severity of nonalcoholic steatohepatitis in hamsters infected with Opisthorchis viverrini
Chutima Sitthirach, Lakhanawan Charoensuk, Chawalit Pairojkul, Apisit Chaidee, Kitti Intuyod, Thatsanapong Pongking, Phonpilas Thongpon, Chanakan Jantawong, Nuttanan Hongsrichan, Sakda Waraasawapati, Manachai Yingklang, Somchai Pinlaor, Michael W. Greene
PLOS ONE.2022; 17(9): e0275273. CrossRef
Association of Red Blood Cell Life Span with Abnormal Changes in Cardiac Structure and Function in Non-Dialysis Patients with Chronic Kidney Disease Stages 3–5
Siyi Rao, Jing Zhang, Jiaqun Lin, Jianxin Wan, Yi Chen
Journal of Clinical Medicine.2022; 11(24): 7373. CrossRef
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Phuphitchan Rachprakhon, Watchariya Purivirojkul
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The American Journal of Tropical Medicine and Hygiene.2020; 102(1): 110. CrossRef
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International Journal of Environmental Research and Public Health.2020; 17(11): 4108. CrossRef
Reduction of Reinfection Rates with Opisthorchis viverrini through a Three-Year Management Program in Endemic Areas of Northeastern Thailand
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The Korean Journal of Parasitology.2020; 58(5): 527. CrossRef
Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity
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eLife.2019;[Epub] CrossRef
A comprehensive review of omics and host-parasite interplays studies, towards control of Opisthorchis viverrini infection for prevention of cholangiocarcinoma
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Modeling liver fluke transmission in northeast Thailand: Impacts of development, hydrology, and control
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Acta Tropica.2018; 188: 101. CrossRef
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New locality record for Haplorchoides mehrai and possible interactions with Opisthorchis viverrini metacercariae in cyprinid fishes in Northeast Thailand
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Parasitology Research.2017; 116(2): 601. CrossRef
Effects of albendazole, artesunate, praziquantel and miltefosine, on Opisthorchis viverrini cercariae and mature metacercariae
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Asian Pacific Journal of Tropical Medicine.2017; 10(2): 126. CrossRef
Ultrastructural characters of the spermatozoon of the liver fluke Opisthorchis viverrini (Poirier, 1886) (Opisthorchiidae)
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Parasitology Research.2017; 116(9): 2499. CrossRef
Epidemiology of Opisthorchis viverrini in an endemic area of Thailand, an integrative approach
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Helminthologia.2017; 54(4): 298. CrossRef
Contamination of Opisthorchis viverrini and Haplorchis taichui metacercariae in fermented fish products in northeastern Thailand markets
Sudarat Onsurathum, Porntip Pinlaor, Lakhanawan Charoensuk, Ornuma Haonon, Apisit Chaidee, Kitti Intuyod, Porntip Laummaunwai, Thidarut Boonmars, Wanlop Kaewkes, Somchai Pinlaor
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Effects of fermentation time and low temperature during the production process of Thai pickled fish (pla-som) on the viability and infectivity of Opisthorchis viverrini metacercariae
Sudarat Onsurathum, Porntip Pinlaor, Ornuma Haonon, Apisit Chaidee, Lakhanawan Charoensuk, Kitti Intuyod, Thidarut Boonmars, Porntip Laummaunwai, Somchai Pinlaor
International Journal of Food Microbiology.2016; 218: 1. CrossRef
Comparison of infectivity, metacercarial burden and host mortality induced byOpisthorchis viverrinisensu lato cercariae from Lao PDR compared with Thailand in cyprinid fish,Barbonymus gonionotus
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Chronic Opisthorchis viverrini Infection Changes the Liver Microbiome and Promotes Helicobacter Growth
Upsornsawan Itthitaetrakool, Porntip Pinlaor, Somchai Pinlaor, Chariya Chomvarin, Rungtiwa Dangtakot, Apisit Chaidee, Chotechana Wilailuckana, Arunnee Sangka, Aroonlug Lulitanond, Puangrat Yongvanit, Paulo Lee Ho
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Asian Pacific Journal of Tropical Disease.2015; 5: S96. CrossRef

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Curcumin Prevents Bile Canalicular Alterations in the Liver of Hamsters Infected with Opisthorchis viverrini: Prapaporn Jattujan, Somchai Pinlaor, Lakhanawan Charoensuk, Channarong Arunyanart, Jariya Umka Welbat, Wunnee Chaijaroonkhanarak; Korean J Parasitol 2013;51(6):695-701.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.695

Abstract
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Opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. Little is known about the pathological alterations in bile canaliculi in opisthorchiasis. This study aimed to investigate bile canalicular alterations in O. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. Hamsters were infected with O. viverrini and one group of animals was fed with 1% dietary curcumin supplement. Animals were examined during the acute infection phase, days 21 and 30 post-infection (PI) and chronic infection phase (day 90 PI). Scanning electron microscopy revealed that in the infected group fed with a normal diet, bile canaliculi became slightly tortuous by 30 day PI and more tortuous at day 90 PI. Transmission electron microscopy showed a reduction in microvilli density of canaliculi starting at day 30 PI, with a marked loss of microvilli at day 90 PI. These ultrastructral changes were slightly seen at day 21 PI, which was similar to that found in infected animals fed with 1% curcumin-supplemented diet. Notably, curcumin treatment prevented the reduction of microvilli density, reduced the dilation of bile canaliculi, and decreased the tortuosity of the bile canaliculi relative to non-infected animals on a normal diet at days 30 and 90 PI. These results suggest that curcumin reduces alteration of bile canaliculi and may be a promising agent to prevent the onset of bile duct abnormalities induced by O. viverrini infection.

Citations

Citations to this article as recorded by

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The American Journal of Chinese Medicine.2024; 52(02): 387. CrossRef
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Zhen Cheng, Yuehua Wang, Bin Li
Journal of Agricultural and Food Chemistry.2022; 70(35): 10708. CrossRef
Generation of oxidative stress and induction of apoptotic like events in curcumin and thymoquinone treated adult Fasciola gigantica worms
Abdur Rehman, Rizwan Ullah, Divya Gupta, M.A. Hannan Khan, Lubna Rehman, Mirza Ahmar Beg, Asad U. Khan, S.M.A. Abidi
Experimental Parasitology.2020; 209: 107810. CrossRef
Nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in Opisthorchis viverrini-infected hamsters
Lakhanawan Charoensuk, Porntip Pinlaor, Supason Wanichwecharungruang, Kitti Intuyod, Kulthida Vaeteewoottacharn, Apisit Chaidee, Puangrat Yongvanit, Chawalit Pairojkul, Natthakitta Suwannateep, Somchai Pinlaor
Nanomedicine: Nanotechnology, Biology and Medicine.2016; 12(1): 21. CrossRef
Bile canalicular changes and defective bile secretion in Opisthorchis viverrini-infected hamsters
Lakhanawan Charoensuk, Porntip Pinlaor, Umawadee Laothong, Puangrat Yongvanit, Chawalit Pairojkul, Yukifumi Nawa, Somchai Pinlaor
Folia Parasitologica.2014; 61(6): 512. CrossRef

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Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp: Ratchadawan Aukkanimart, Thidarut Boonmars, Somchai Pinlaor, Smarn Tesana, Surasit Aunpromma, Chantana Booyarat, Pranee Sriraj, Porntip Laummaunwai, Wiyada Punjaruk; Korean J Parasitol 2013;51(5):537-544.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.537

Abstract
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The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC₅₀) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC₅₀ at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides.

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