Parasites, Hosts and Diseases

"infectivity"

Brief Communication

Comparison of Resistance to γ-Irradiation between Cryptosporidium parvum and Cryptosporidium muris Using In Vivo Infection: Sejoung Yoon, Jae-Ran Yu; Korean J Parasitol 2011;49(4):423-426.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.423

Abstract
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In the genus Cryptosporidium, there are more than 14 species with different sizes and habitats, as well as different hosts. Among these, C. parvum and C. hominis are known to be human pathogens. As C. parvum can survive exposure to harsh environmental conditions, including various disinfectants or high doses of radiation, it is considered to be an important environmental pathogen that may be a threat to human health. However, the resistance of other Cryptosporidium species to various environmental conditions is unknown. In this study, resistance against γ-irradiation was compared between C. parvum and C. muris using in vivo infection in mice. The capability of C. muris to infect mice could be eliminated with 1,000 Gy of γ-irradiation, while C. parvum remained infective in mice after up to 1,000 Gy of γ-irradiation, although the peak number of oocysts per gram of feces decreased to 16% that of non-irradiated oocysts. The difference in radioresistance between these 2 Cryptosporidium species should be investigated by further studies.

Citations

Citations to this article as recorded by

Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii
Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert
Parasitology Research.2023; 122(8): 1819. CrossRef
Recombinant thioredoxin peroxidase from Cryptosporidium parvum has more powerful antioxidant activity than that from Cryptosporidium muris
Sejoung Yoon, Woo-Yoon Park, Jae-Ran Yu
Experimental Parasitology.2012; 131(3): 333. CrossRef

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62 Download
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Original Article

Quantitative Evaluation of Infectivity Change of Cryptosporidium parvum after Gamma Irradiation: Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu; Korean J Parasitol 2009;47(1):7-11.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.7

Abstract
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Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C. parvum after gamma irradiation and repair of the infectivity during incubation time after irradiation. C. parvum oocysts were subjected to gamma irradiation at various doses (1, 5, 10, and 25 kGy), and the in vitro infectivity was measured by real-time PCR every day up to 7 days after irradiation. The in vitro infectivity of C. parvum on human ileocecal adenocarcinoma cells (HCT-8) was effectively reduced (> 2 log₁₀) by irradiation at 10 kGy or more. However, in the experiment to find out repair of the infectivity, recovery was not noted until day 7 post-incubation.

Citations

Citations to this article as recorded by

Ultrastructural Changes inCryptosporidium parvumOocysts by Gamma Irradiation
Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu
The Korean Journal of Parasitology.2011; 49(1): 25. CrossRef
Effect of Infrared Irradiation of Oocysts on the Pathology of Experimental Cryptosporidiosis in Apparently Normal and Immunosuppressed Mice
Hafidh I. Al-Sadi, Saevan S. Al-Mahm
Research Journal of Biological Sciences.2010; 5(2): 177. CrossRef
Cryptosporidiosis
Jae-Ran Yu
Hanyang Medical Reviews.2010; 30(3): 187. CrossRef
Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay
Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu
Experimental Parasitology.2010; 125(3): 230. CrossRef

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Brief Communications

Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time: Rosangela Soares Uzeda, Kattyanne De Souza Costa, Sara Lima Santos, Alexandre Moraes Pinheiro, Maria Angela Ornelas De Almeida, Milton M. McAllister, Luis Fernando Pita Gondim; Korean J Parasitol 2007;45(4):295-299.
Published online December 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.4.295

Abstract
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The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4℃, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1: 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.

Citations

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Serological study of Neospora caninum in dogs and wildlife in a nature conservation area in southern Portugal
H. WAAP, T. NUNES, Y. VAZ, A. LEITÃO
Parasitology Open.2017;[Epub] CrossRef
All about neosporosis in Brazil
Camila Koutsodontis Cerqueira-Cézar, Rafael Calero-Bernal, Jitender Prakash Dubey, Solange Maria Gennari
Revista Brasileira de Parasitologia Veterinária.2017; 26(3): 253. CrossRef
Neosporosis in animals—The last five years
J.P. Dubey, G. Schares
Veterinary Parasitology.2011; 180(1-2): 90. CrossRef

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Effects of exogenous glucose on survival and infectivity of Schistosoma mansoni cercariae: Bernard Fried, Robert Laterra, Yonghyun Kim; Korean J Parasitol 2002;40(1):55-58.
Published online March 31, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.1.55

Abstract
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The effects of exogenous glucose in artificial spring water (ASW) were studied on the survival and infectivity of Schistosoma mansoni cercariae. The mean percent survival of cercariae maintained in 1% glucose in ASW for 36 and 48hr was significantly greater than that of cercariae maintained identically in ASW. Cercariae maintained in ASW with or without glucose for 24hr, fixed in neutral buffered formalin, and stained in Oil Red O, showed an accumulation of neutral lipid in the tail. Cercariae maintained as described above and stained in periodic acid-Schiff exhibited depleted glycogen, mainly from the tail. Cercariae maintained in ASW with glucose for 24hr did not resynthesize glycogen. Cercariae maintained in ASW with glucose for 24hr were as capable of infecting male FVBN202 mice as were freshly emerged cercariae, and increased the percent of worm recovery. Exogeneous glucose added to ASW prolonged the survival of S. mansoni cercariae and increased infectivity in terms of worm recovery.

Citations

Citations to this article as recorded by

Heads or tails? Differential translational regulation in cercarial heads and tails of schistosome worms
James R. Hagerty, Emmitt R. Jolly, Salah A. Sheweita
PLOS ONE.2019; 14(10): e0224358. CrossRef
In Vitro Maintenance of Clonorchis sinensis Adult Worms
Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2012; 50(4): 309. CrossRef
Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media
Shunyu Li, Tae Im Kim, Won Gi Yoo, Pyo Yun Cho, Tong-Soo Kim, Sung-Jong Hong
Parasitology Research.2008; 103(5): 1019. CrossRef
Effects of glucose on survival, infectivity and linear movement of the cercariae of Echinostoma caproni
E.L. Ponder, B. Fried
Journal of Helminthology.2004; 78(2): 185. CrossRef
Emergence of cercariae of Echinostoma caproni and Schistosoma mansoni from Biomphalaria glabrata under different laboratory conditions
B. Fried, R. Laterra, Y. Kim
Journal of Helminthology.2002; 76(4): 369. CrossRef

7,442 View
102 Download
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Original Article

Effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae: M. Khyrul Islam, Kiku Matsuda, Jin-Ho Kim, Byeong-Kirl Baek; Korean J Parasitol 1999;37(1):13-19.
Published online March 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.1.13

Abstract
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The effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae (L₃) to rats were investigated. A significantly higher body length was observed in L₃ from filter paper culture (597.3 ± 32.2 ?m) than those in fecal (509.9 ± 35.0 ?m) and nutrient broth culture (503.3 ± 31.0 ?m) (P<0.05). Larval infectivity was assessed by exposing rats to 1,000 L₃ from each culture and worms were recovered from the lungs and small intestines. Recovery rate of these worms did not show any significant difference. A significantly greater body length of adults was recorded in those corresponding to the L₃ harvested from filter paper (2,777.5 ± 204.4 ?m) and nutrient broth culture (2,732.5 ± 169.8 ?m) than those corresponding to the L₃ obtained from fecal culture (2,600.5 ± 172.4 ?m) (P<0.05). Although worm fecundity and EPG counts differed among culture methods but worm burdens and course of infection did not. These findings suggest that the methods of cultures have a significant effect on the morphological development of the larvae to the L₃ stage, but do not influence the infectivity to rats.

Citations

Citations to this article as recorded by

Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration
Daigo Tsubokawa, Taisei Kikuchi, Jae Man Lee, Takahiro Kusakabe, Yasuhiko Yamamoto, Haruhiko Maruyama, James B. Lok
PLOS Pathogens.2021; 17(6): e1009649. CrossRef
Strongyloides ratti and S. venezuelensis – rodent models of Strongyloides infection
MARK VINEY, TAISEI KIKUCHI
Parasitology.2017; 144(3): 285. CrossRef
Venestatin, a Ca ++ -binding protein from the parasitic nematode Strongyloides venezuelensis , is involved in the larval migration process
Daigo Tsubokawa, Takeshi Hatta, Taisei Kikuchi, Hiroki Maeda, Fusako Mikami, M. Abdul Alim, Haruhiko Maruyama, Naotoshi Tsuji
International Journal for Parasitology.2017; 47(8): 501. CrossRef
Viability of Strongyloides venezuelensis eggs and larvae in vermiculite containing the fungus Duddingtonia flagrans
Laryssa Pinheiro Costa Silva, Carolina Magri Ferraz, Anderson Rocha Aguiar, Jackson Victor Araújo, Steveen Rios Ribeiro, Débora Goldner Rossi, Luanderson Queiroz Mendes, Fausto Edmundo Lima Pereira, Narcisa Imaculada Brant Moreira, Fabio Ribeiro Braga
Parasitology Research.2017; 116(7): 2047. CrossRef
Development of free-living stages of Strongyloides ratti under different temperature conditions
Maki Sakamoto, Shoji Uga
Parasitology Research.2013; 112(12): 4009. CrossRef
Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis
Eiji Nagayasu, Yoshitoshi Ogura, Takehiko Itoh, Ayako Yoshida, Gunimala Chakraborty, Tetsuya Hayashi, Haruhiko Maruyama
Parasitology International.2013; 62(1): 57. CrossRef
Characterization of the protective response against a homologous challenge infection with Strongyloides venezuelensis in rats
B.K Baek, M.K Islam, B.S Kim, C.W Lim, J Hur, A.O Oluoch, C.H Kim, I Kakoma
Veterinary Parasitology.2003; 113(3-4): 217. CrossRef
Persistent infection with Strongyloides venezuelensis in the Mongolian gerbil (Meriones unguiculatus)
Byeong-Kirl Baek, In-Soo Whang, M. Khyrul Islam, Byeong-Soo Kim, Ibulaimu Kakoma
The Korean Journal of Parasitology.2002; 40(4): 181. CrossRef
Partial cross-resistance between Strongyloides venezuelensis and Nippostrongylus brasiliensis in rats
Byeong-Kirl Baek, M. Khyrul Islam, Jin-Ho Kim, John-Wha Lee, Jin Hur
The Korean Journal of Parasitology.1999; 37(2): 101. CrossRef