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"mitochondrial DNA"

Original Article

Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)
Ze-Xuan Liu, Yan Zhang, Yu-Ting Liu, Qiao-Cheng Chang, Xin Su, Xue Fu, Dong-Mei Yue, Yuan Gao, Chun-Ren Wang
Korean J Parasitol 2016;54(2):173-179.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.173
Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

Citations

Citations to this article as recorded by  Crossref logo
  • Genetic variation and population structure of Haemonchus contortus: an in-silico analysis
    W. Wei, Z. Lan, Xuewei Liu, Xinhui Zhang, X. Gu, R. Wang
    Journal of Helminthology.2025;[Epub]     CrossRef
  • The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
    Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
    Biology.2025; 14(8): 1101.     CrossRef
  • The complete mitochondrial genome of Aspidogaster ijimai (Platyhelminthes: Trematoda: Aspidogastrea): gene content and phylogenetic inference
    D. A. Solodovnik, D. M. Atopkin, A. A. Semenchenko, M. Urabe, S. G. Sokolov
    Invertebrate Zoology.2025; 22(3): 411.     CrossRef
  • Complete mitochondrial genome and phylogenetic analysis of Dollfustrema vaneyi (Trematoda: Bucephalidae)
    Ye Hu, Tong Ye, Hong Zou, Gui-Tang Wang, Wen-Xiang Li, Dong Zhang
    BMC Genomics.2024;[Epub]     CrossRef
  • Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications
    Janelle Laura J. Gacad, Natalia I. Yurlova, Natalia M. Ponomareva, Misako Urabe
    Parasitology Research.2023; 122(7): 1545.     CrossRef
  • A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
    D. M. Atopkin, A. A. Semenchenko, D. A. Solodovnik, Y. I. Ivashko
    Journal of Helminthology.2023;[Epub]     CrossRef
  • The complete mitochondrial genome of Prosthogonimus cuneatus and Prosthogonimus pellucidus (Trematoda: Prosthogonimidae), their features and phylogenetic relationships in the superfamily Microphalloidea
    Xin-ru Guo, Ye Li, Yuan Gao, Yang-yuan Qiu, Zhen-hua Jin, Zhong-yan Gao, Xian-guang Zhang, Qi An, Qiao-cheng Chang, Jun-feng Gao, Chun-ren Wang
    Acta Tropica.2022; 232: 106469.     CrossRef
  • Characterization of complete mitochondrial genome and ribosomal operon forCarassotrema koreanumPark, 1938 (Digenea: Haploporidae) by means of next-generation sequencing data
    Y.I. Ivashko, A.A. Semenchenko, D.A. Solodovnik, D.M. Atopkin
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Trematode diversity in freshwater snails from a stopover point for migratory waterfowls in Hokkaido, Japan: An assessment by molecular phylogenetic and population genetic analyses
    Minoru Nakao, Mizuki Sasaki
    Parasitology International.2021; 83: 102329.     CrossRef
  • First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971
    Dmitry M. Atopkin, Alexander A. Semenchenko, Daria A. Solodovnik, Yana I. Ivashko, Kirill A. Vinnikov
    Parasitology Research.2021; 120(6): 2037.     CrossRef
  • Characterization of the complete mitochondrial genome sequence of Tracheophilus cymbius (Digenea), the first representative from the family Cyclocoelidae
    Y. Li, X.X. Ma, Q.B. Lv, Y. Hu, H.Y. Qiu, Q.C. Chang, C.R. Wang
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Mitochondrial Genome Sequence of Echinostoma revolutum from Red-Crowned Crane (Grus japonensis)
    Rongkun Ran, Qi Zhao, Asmaa M. I. Abuzeid, Yue Huang, Yunqiu Liu, Yongxiang Sun, Long He, Xiu Li, Jumei Liu, Guoqing Li
    The Korean Journal of Parasitology.2020; 58(1): 73.     CrossRef
  • Characterization and comparative analysis of the complete mitochondrial genome of Azygia hwangtsiyui Tsin, 1933 (Digenea), the first for a member of the family Azygiidae
    Yuan-An Wu, Jin-Wei Gao, Xiao-Fei Cheng, Min Xie, Xi-Ping Yuan, Dong Liu, Rui Song
    ZooKeys.2020; 945: 1.     CrossRef
  • Characterization of the complete mitochondrial genome of Diplostomum baeri
    Toby Landeryou, Stephen M. Kett, Anne Ropiquet, Dirk Wildeboer, Scott P. Lawton
    Parasitology International.2020; 79: 102166.     CrossRef
  • A fine‐scale phylogenetic assessment of digenean trematodes in central Alberta reveals we have yet to uncover their total diversity
    Michelle A. Gordy, Patrick C. Hanington
    Ecology and Evolution.2019; 9(6): 3153.     CrossRef
  • Characterization of the complete mitochondrial genome of Plagiorchis maculosus (Digenea, Plagiorchiidae), Representative of a taxonomically complex digenean family
    Suleman, Jun Ma, Mian Sayed Khan, Vasyl V. Tkach, Nehaz Muhammad, Dong Zhang, Xing-Quan Zhu
    Parasitology International.2019; 71: 99.     CrossRef
  • Characterization of the mitochondrial genome sequences of the liver fluke Amphimerus sp. (Trematoda: Opisthorchiidae) from Ecuador and phylogenetic implications
    Jun Ma, Jun-Jun He, Cheng-Yan Zhou, Miao-Miao Sun, William Cevallos, Hiromu Sugiyama, Xing-Quan Zhu, Manuel Calvopiña
    Acta Tropica.2019; 195: 90.     CrossRef
  • Characterization of the complete mitochondrial genome of Uvitellina sp., representative of the family Cyclocoelidae and phylogenetic implications
    Suleman, Mian Sayed Khan, Petr Heneberg, Cheng-Yan Zhou, Nehaz Muhammad, Xing-Quan Zhu, Jun Ma
    Parasitology Research.2019; 118(7): 2203.     CrossRef
  • The complete mitochondrial genome of Echinostoma miyagawai: Comparisons with closely related species and phylogenetic implications
    Ye Li, Yang-Yuan Qiu, Min-Hao Zeng, Pei-Wen Diao, Qiao-Cheng Chang, Yuan Gao, Yan Zhang, Chun-Ren Wang
    Infection, Genetics and Evolution.2019; 75: 103961.     CrossRef
  • Mitochondrial DNA Evidence Supports the Hypothesis that Triodontophorus Species Belong to Cyathostominae
    Yuan Gao, Yan Zhang, Xin Yang, Jian-Hua Qiu, Hong Duan, Wen-Wen Xu, Qiao-Cheng Chang, Chun-Ren Wang
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • 11,745 View
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  • 22 Web of Science
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Mini Review

Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
Korean J Parasitol 2009;47(Suppl):S21.
Published online October 26, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S21

After morphological grouping of Acanthamoeba by Pussard and Pons, phylogeny of the genus has been always a big topic to the researchers. Because of the variability of morphological characteristics, unchangeable and stable characters have been investigated for phylogenic criteria. Isoenzyme and mitochondrial DNA RFLP (Mt DNA RFLP) analyses revealed different patterns among strains assigned to a same species. Therefore, these characteristics would be considered as tools for strain discrimination than species identification. The most recently developed and the most promising method is the sequence analysis of 18s ribosomal RNA coding DNA (18s rDNA). The phylogenic tree based on comparison of 18s rDNA sequences distinguished the 3 morphological groups of Acanthamoeba and divided them into 12 unique sequence types (T1-T12 genotypes). Most clinical and environmental isolates belonged to the morphological group II and the genotype T4. In the Republic of Korea, 2 strains of Acanthamoeba, YM-2 and YM-3, were first isolated from the environment in 1974. However, phylogenic identification of Korean Acanthamoeba isolates from human infections or the environment were tried from the late 1990s. By RFLP analysis or total sequence analysis of 18s rDNA revealed that almost all clinical isolates including the one from a suspicious granulomatous amebic encephalitis patient belonged to the genotype T4. A large number of environmental isolates from contact lens storage cases, tapped water, and ocean sediments also belonged to the genotype T4. Almost identical strain characteristics, such as Mt DNA RFLP pattern of environmental isolates, with the clinical isolates could make a simple conclusion that most environmental isolates might be a potential keratopathogen.

Citations

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  • Insights Into the Genetic Diversity of Acanthamoeba Keratitis: A Clinical and Molecular Study
    Vinil K. Kavya, Lakshmipathy Dhanurekha, Ramachandran Abirami, Ranganathan Sampathkumar, Agarwal S. Shweta, Appakkudal R. Anand, Sri B. Madhangi
    Cornea.2025;[Epub]     CrossRef
  • Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
    Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
    Pathogens.2024; 13(2): 142.     CrossRef
  • A Narrative Review of Acanthamoeba Isolates in Malaysia: Challenges in Infection Management and Natural Therapeutic Advancements
    Mohammad Wisman Abdul Hamid, Roslaini Bin Abd Majid, Victor Fiezal Knight Victor Ernest, Nik Noorul Shakira Mohamed Shakrin, Firdaus Mohamad Hamzah, Mainul Haque
    Cureus.2024;[Epub]     CrossRef
  • Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer
    Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
    Journal of Water and Health.2024; 22(12): 2289.     CrossRef
  • Biological characteristics and pathogenicity of Acanthamoeba
    Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Shotgun Kinetic Target-Guided Synthesis Approach Enables the Discovery of Small-Molecule Inhibitors against Pathogenic Free-Living Amoeba Glucokinases
    Mintesinot Kassu, Prakash T. Parvatkar, Jillian Milanes, Neil P. Monaghan, Chungsik Kim, Matthew Dowgiallo, Yingzhao Zhao, Ami H. Asakawa, Lili Huang, Alicia Wagner, Brandon Miller, Karissa Carter, Kayleigh F. Barrett, Logan M. Tillery, Lynn K. Barrett, I
    ACS Infectious Diseases.2023; 9(11): 2190.     CrossRef
  • Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea
    Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin
    Scientific Reports.2020;[Epub]     CrossRef
  • Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
    Stas Malavin, Lyubov Shmakova
    European Journal of Protistology.2020; 73: 125671.     CrossRef
  • Isolation and molecular characterization of Acanthamoeba from patients with keratitis in Spain
    T. Martín-Pérez, A. Criado-Fornelio, J. Martínez, M.A. Blanco, I. Fuentes, J. Pérez-Serrano
    European Journal of Protistology.2017; 61: 244.     CrossRef
  • Free-living amoebae in the water resources of Iran: a systematic review
    Ehsan Saburi, Toktam Rajaii, Asma Behdari, Mohammad Hasan Kohansal, Hossein Vazini
    Journal of Parasitic Diseases.2017; 41(4): 919.     CrossRef
  • Genotyping of Acanthamoeba spp. from water sources from Northwestern Iran
    Ali Haniloo, Ali Pezeshki, Abbas Mahmmodzadeh, Elnaz Kadkhodamohammadi
    Acta Parasitologica.2017;[Epub]     CrossRef
  • Pathogenic free-living amoeba
    Natalia Łanocha-Arendarczyk, Danuta Kosik-Bogacka, Katarzyna Galant, Wojciech Zaorski, Karolina Kot, Aleksandra Łanocha
    Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(1): 106.     CrossRef
  • Metacommunity analysis of amoeboid protists in grassland soils
    Anna Maria Fiore-Donno, Jan Weinert, Tesfaye Wubet, Michael Bonkowski
    Scientific Reports.2016;[Epub]     CrossRef
  • Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt
    Gihan Mostafa Tawfeek, Sawsan Abdel-Hamid Bishara, Rania Mohammad Sarhan, Eman ElShabrawi Taher, Amira ElSaady Khayyal
    Parasitology Research.2016; 115(5): 1871.     CrossRef
  • Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary
    Csaba Kiss, Zsófia Barna, Márta Vargha, Júlia Katalin Török
    Parasitology Research.2014; 113(7): 2551.     CrossRef
  • Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
    Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
    The Korean Journal of Parasitology.2013; 51(4): 401.     CrossRef
  • Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil
    Daniella de Sousa Mendes Moreira Alves, Aline Silva Moraes, Nadjar Nitz, Mayara Gabriele Carvalho de Oliveira, Mariana Machado Hecht, Rodrigo Gurgel-Gonçalves, César Augusto Cuba Cuba
    Experimental Parasitology.2012; 131(2): 239.     CrossRef
  • Exploring the Unique N-Glycome of the Opportunistic Human Pathogen Acanthamoeba
    Birgit Schiller, Georgia Makrypidi, Ebrahim Razzazi-Fazeli, Katharina Paschinger, Julia Walochnik, Iain B.H. Wilson
    Journal of Biological Chemistry.2012; 287(52): 43191.     CrossRef
  • Comparison of molecular diagnostic methods for the detection of Acanthamoeba spp. from clinical specimens submitted for keratitis
    Krishna Khairnar, Gurdip S. Tamber, Filip Ralevski, Dylan R. Pillai
    Diagnostic Microbiology and Infectious Disease.2011; 70(4): 499.     CrossRef
  • 19,252 View
  • 194 Download
  • Crossref

Brief Communications

Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis
Soo-Ung Lee, Sun Huh
Korean J Parasitol 2004;42(3):145-148.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.145

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

Citations

Citations to this article as recorded by  Crossref logo
  • Genetic Variation and Population Structure of Clonorchis sinensis: An In Silico Analysis
    Xinhui Zhang, Zhuo Lan, Wei Wei, Aihui Zhang, Hongyu Qiu, Junfeng Gao, Chunren Wang
    Pathogens.2024; 13(11): 991.     CrossRef
  • Black spot diseases in seven commercial fish species from the English Channel and the North Sea: infestation levels, identification and population genetics of Cryptocotyle spp.
    Maureen Duflot, Pierre Cresson, Maéva Julien, Léa Chartier, Odile Bourgau, Marialetizia Palomba, Simonetta Mattiucci, Graziella Midelet, Mélanie Gay
    Parasite.2023; 30: 28.     CrossRef
  • Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report
    Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang
    Acta Parasitologica.2022; 67(1): 316.     CrossRef
  • Ancient DNA of Metagonimus yokogawai Recovered from Joseon Period Human Remains Newly Discovered at Goryeong County in South Korea
    Chang Seok Oh, Jong Ha Hong, Jong Yil Chai, Mi Kyung Song, Ho-Jin Jang, Min Seo, Dong Hoon Shin
    Acta Parasitologica.2022; 67(1): 539.     CrossRef
  • Genetic Analysis of Ancient Clonorchis sinensis Eggs Attained from Goryeong Mummy of Joseon Dynasty Period
    Chang Seok Oh, Min Seo, Hye Jin Lee, Myeung Ju Kim, Do-Seon Lim, Dong Hoon Shin
    Journal of Parasitology.2022;[Epub]     CrossRef
  • Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel
    Maureen Duflot, Mélanie Gay, Graziella Midelet, Per Walter Kania, Kurt Buchmann
    Parasitology Research.2021; 120(10): 3417.     CrossRef
  • The SSU rRNA secondary structures of the Plagiorchiida species (Digenea), its applications in systematics and evolutionary inferences
    A.N. Voronova, G.N. Chelomina
    Infection, Genetics and Evolution.2020; 78: 104042.     CrossRef
  • Marked mitochondrial genetic variation in individuals and populations of the carcinogenic liver fluke Clonorchis sinensis
    Liina Kinkar, Pasi K. Korhonen, Daxi Wang, Xing-Quan Zhu, Galina N. Chelomina, Tao Wang, Ross S. Hall, Anson V. Koehler, Ivon Harliwong, Bicheng Yang, J. Lynn Fink, Neil D. Young, Robin B. Gasser, David Blair
    PLOS Neglected Tropical Diseases.2020; 14(8): e0008480.     CrossRef
  • Molecular Identification and Phylogenetic Analysis of Nuclear rDNA Sequences of Clonorchis sinensis Isolates From Human Fecal Samples in Heilongjiang Province, China
    Xiaoli Zhang, Beibei Sun, Qiaoran Tang, Rui Chen, Su Han
    Frontiers in Microbiology.2019;[Epub]     CrossRef
  • Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
    Jong Ha Hong, Chang Seok Oh, Jong-Yil Chai, Min Seo, Dong Hoon Shin
    Journal of Korean Medical Science.2019;[Epub]     CrossRef
  • Palaeoparasitology and palaeogenetics: review and perspectives for the study of ancient human parasites
    NATHALIE M.-L. CÔTÉ, MATTHIEU LE BAILLY
    Parasitology.2018; 145(5): 656.     CrossRef
  • Improved genomic resources and new bioinformatic workflow for the carcinogenic parasite Clonorchis sinensis: Biotechnological implications
    Daxi Wang, Pasi K. Korhonen, Robin B. Gasser, Neil D. Young
    Biotechnology Advances.2018; 36(4): 894.     CrossRef
  • Molecular and phylogenetic analyses of the liver amphistome Explanatum explanatum (Creplin, 1847) Fukui, 1929 in ruminants from Bangladesh and Nepal based on nuclear ribosomal ITS2 and mitochondrial nad1 sequences
    U.K. Mohanta, H.B. Rana, B. Devkota, T. Itagaki
    Journal of Helminthology.2017; 91(4): 497.     CrossRef
  • Mitochondrial genomic comparison of Clonorchis sinensis from South Korea with other isolates of this species
    Daxi Wang, Neil D. Young, Anson V. Koehler, Patrick Tan, Woon-Mok Sohn, Pasi K. Korhonen, Robin B. Gasser
    Infection, Genetics and Evolution.2017; 51: 160.     CrossRef
  • Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples
    S. M. Mazidur Rahman, Hyun Beom Song, Yan Jin, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Min-Ho Choi, Justin V. Remais
    PLOS Neglected Tropical Diseases.2017; 11(10): e0005995.     CrossRef
  • Inter-individual and intragenomic variations in the ITS region of Clonorchis sinensis (Trematoda: Opisthorchiidae) from Russia and Vietnam
    Yulia V. Tatonova, Galina N. Chelomina, Hung Manh Nguyen
    Infection, Genetics and Evolution.2017; 55: 350.     CrossRef
  • Current status ofClonorchis sinensisand clonorchiasis in China
    De-Hua Lai, Xiao-Kun Hong, Bi-Xiu Su, Chi Liang, Geoff Hide, Xiaoli Zhang, Xinbing Yu, Zhao-Rong Lun
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 21.     CrossRef
  • Genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke Clonorchis sinensis
    Thao T. B. Nguyen, Yuji Arimatsu, Sung-Jong Hong, Paul J. Brindley, David Blair, Thewarach Laha, Banchob Sripa
    Parasitology Research.2015; 114(6): 2263.     CrossRef
  • A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region
    K. Choudhary, A. Kumar Verma, S. Swaroop, N. Agrawal
    Helminthologia.2015; 52(3): 167.     CrossRef
  • Genetic diversity of the Chinese liver fluke Clonorchis sinensis from Russia and Vietnam
    Galina N. Chelomina, Yulia V. Tatonova, Nguyen Manh Hung, Ha Duy Ngo
    International Journal for Parasitology.2014; 44(11): 795.     CrossRef
  • Roles of liver fluke infection as risk factor for cholangiocarcinoma
    Paiboon Sithithaworn, Puangrat Yongvanit, Kunyarat Duenngai, Nadda Kiatsopit, Chawalit Pairojkul
    Journal of Hepato-Biliary-Pancreatic Sciences.2014; 21(5): 301.     CrossRef
  • Genetic variation amongClonorchis sinensisisolates from different hosts and geographical locations revealed by sequence analysis of mitochondrial and ribosomal DNA regions
    Jing-Ying Xiao, Jun-Feng Gao, Lian-Shun Cai, Yue Dai, Chun-Jia Yang, Lan Luo, Takeshi Agatsuma, Chun-Ren Wang
    Mitochondrial DNA.2013; 24(5): 559.     CrossRef
  • The zoonotic, fish-borne liver flukes Clonorchis sinensis, Opisthorchis felineus and Opisthorchis viverrini
    Trevor N. Petney, Ross H. Andrews, Weerachai Saijuntha, Alexandra Wenz-Mücke, Paiboon Sithithaworn
    International Journal for Parasitology.2013; 43(12-13): 1031.     CrossRef
  • Genetic diversity of Clonorchis sinensis (Trematoda: Opisthorchiidae) in the Russian southern Far East based on mtDNA cox1 sequence variation
    Yulia V. Tatonova, Galina N. Chelomina, Vladimir V. Besprozvannykh
    Folia Parasitologica.2013; 60(2): 155.     CrossRef
  • Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
    Jiufeng Sun, Yan Huang, Huaiqiu Huang, Pei Liang, Xiaoyun Wang, Qiang Mao, Jingtao Men, Wenjun Chen, Chuanhuan Deng, Chenhui Zhou, Xiaoli Lv, Juanjuan Zhou, Fan Zhang, Ran Li, Yanli Tian, Huali Lei, Chi Liang, Xuchu Hu, Jin Xu, Xuerong Li, XinbingYu, Dav
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  • Microscopic Examination of Gallbladder Stones Improves Rate of Detection of Clonorchis sinensis Infection
    Tie Qiao, Rui-hong Ma, Xiao-bing Luo, Pei-ming Zheng, Zhen-liang Luo, Liu-qing Yang
    Journal of Clinical Microbiology.2013; 51(8): 2551.     CrossRef
  • Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
    Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
    Parasitology International.2012; 61(1): 71.     CrossRef
  • Genetic diversity of nuclear ITS1–5.8S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East
    Yulia V. Tatonova, Galina N. Chelomina, Vladimir V. Besprosvannykh
    Parasitology International.2012; 61(4): 664.     CrossRef
  • Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis
    Nadda Kiatsopit, Paiboon Sithithaworn, Jiraporn Sithithaworn, Thidarut Boonmars, Smarn Tesana, Opal Pitaksakulrat, Weerachai Saijuntha, Trevor N. Petney, Ross H. Andrews
    Parasitology Research.2011; 108(1): 211.     CrossRef
  • Prevalence of Clonorchis sinensis infection in dogs and cats in subtropical southern China
    Rui-Qing Lin, Jian-Dong Tang, Dong-Hui Zhou, Hui-Qun Song, Si-Yang Huang, Jia-Xu Chen, Mu-Xin Chen, Han Zhang, Xing-Quan Zhu, Xiao-Nong Zhou
    Parasites & Vectors.2011;[Epub]     CrossRef
  • To: Urusa Thaenkham and Yukifumi Nawa: Double Strand Problems: Reverse DNA Sequences Deposited in the DNA Database
    Sun Huh
    The Korean Journal of Parasitology.2010; 48(1): 91.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
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To determine the molecular phylogenic location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenic relationship between members of the Plagiorchiidae, as suggested by morphologic features.

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Original Article

Phylogenetic relationship of ribosomal ITS2 and mitochondrial COI among diploid and triploid Paragonimus westermani isolates
Gab-Man Park, Kyung-Il Im, Tai-Soon Yong
Korean J Parasitol 2003;41(1):47-55.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.47

We compared patterns of intraspecific polymorphism of two markers with contrasting modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the lung fluke, diploid and triploid Paragonimus westermani from three geographical regions of Korea. The genetic distances between three populations of Korean diploid and triploid P. westermani showed no significant difference in the nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (mtCOI) and ribosomaal second internal transcribed spacer (ITS2) genes. A highly resolved strict-consensus tree was obtained that illustrated phylogenetically useful information of the ITS2 and mtCOI sequences from diploid and triploid P. westermani.

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Mini Review

To choose one or more appropriate molecular markers or gene regions for resolving a particular systematic question among the organisms at a certain categorical level is still a very difficult process. The primary goal of this review, therefore, is to provide a theoretical information in choosing one or more molecular markers or gene regions by illustrating general properties and phylogenetic utilities of nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) that have been most commonly used for phylogenetic researches. The highly conserved molecular markers and/or gene regions are useful for investigating phylogenetic relationships at higher categorical levels (deep branches of evolutionary history). On the other hand, the hypervariable molecular markers and/or gene regions are useful for elucidating phylogenetic relationships at lower categorical levels (recently diverged branches). In summary, different selective forces have led to the evolution of various molecular markers or gene regions with varying degrees of sequence conservation. Thus, appropriate molecular markers or gene regions should be chosen with even greater caution to deduce true phylogenetic relationships over a broad taxonomic spectrum.

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