Parasites, Hosts and Diseases

"rapid detection"

Brief Communication

Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces: Yao-Dong Wu, Qi-Qi Wang, Meng Wang, Hany M. Elsheikha, Xin Yang, Min Hu, Xing-Quan Zhu, Min-Jun Xu; Korean J Parasitol 2021;59(2):167-171.
Published online April 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.2.167

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

Citations

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An RPA-CRISPR/Cas12a-assisted method for nucleic acid detection of Haemonchus contortus in sheep
Yutong Cao, Qiankun Yang, Yanbing Guo, Xiaocen Wang, Xin Li, Nan Zhang, Wenxue Lu, Jianhua Li, Xichen Zhang, Lili Cao, Pengtao Gong
Veterinary Parasitology.2025; 334: 110421. CrossRef
Rapid visual detection of Moniezia spp. in sheep feces via Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) assay
Shaohua Zhang, Yeping Zhao, Weijia Liang, Shuai Wang, Xiu Cui, Haohan Zhu, Yueyue Zhang, Xiaolei Liu, Huimin Li, Wenjie Mu, Aijiang Guo
Veterinary Parasitology.2025; 339: 110582. CrossRef
Preliminary results of the recombinase polymerase amplification technique for the detection of Haemonchus contortus from Hungarian field samples
Rojesh Khangembam, Nóra Vass, Alison Morrison, Lynsey A. Melville, Alistair Antonopoulos, Levente Czeglédi
Veterinary Parasitology.2023; 320: 109974. CrossRef

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Original Articles

Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba: Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2013;51(3):269-277.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.269

Abstract
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Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.

Citations

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Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii
Susanna Haapanen, Maarit S. Patrikainen, Seppo Parkkila
Diagnostic Microbiology and Infectious Disease.2023; 107(2): 116014. CrossRef
A simple and visible detection method for the rapid diagnosis of Ustilaginoidea virens in rice seeds by a loop‐mediated isothermal amplification assay
Wei Wang, Hang Yin, Ning Huang, Cuijing Zhu, Yufei Wang, Xintong Qi, Lu Ma, Yunxin Fan, Yao Yu, Hongsheng Zhang, Yongmei Bao
Journal of Phytopathology.2021; 169(6): 369. CrossRef
Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample
Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
Scientific Reports.2021;[Epub] CrossRef
Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis of Plasmodium vivax Malaria in a Tertiary Hospital in Chandigarh, North India
Hargobinder Kaur, Rakesh Sehgal, Devendra Bansal, Ali A. Sultan, Ashish Bhalla, Sunit C. Singhi
The American Journal of Tropical Medicine and Hygiene.2018; 98(5): 1374. CrossRef
Detection of Acanthamoeba spp. in water samples collected from natural water reservoirs, sewages, and pharmaceutical factory drains using LAMP and PCR in China
Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
Science of The Total Environment.2017; 584-585: 489. CrossRef
Water-borne protozoa parasites: The Latin American perspective
Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
International Journal of Hygiene and Environmental Health.2017; 220(5): 783. CrossRef
Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
Indian Journal of Medical Microbiology.2017; 35(1): 90. CrossRef
Acanthamoeba keratitis: improving the Scottish diagnostic service for the rapid molecular detection of Acanthamoeba species
Claire Low Alexander, Michael Coyne, Brian Jones, Deepa Anijeet
Journal of Medical Microbiology .2015; 64(7): 682. CrossRef
Molecular diagnosis in clinical parasitology: When and why?
Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
Experimental Biology and Medicine.2014; 239(11): 1443. CrossRef

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Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand: Wanna Chaijaroenkul, Thanee Wongchai, Ronnatrai Ruangweerayut, Kesara Na-Bangchang; Korean J Parasitol 2011;49(1):33-38.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.33

Abstract
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Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pan™, Malaria Ag-Pf™, and Malaria Ag-Pv™ tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pf™ and Malaria Antigen Pf/Pan™ compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, and Malaria Antigen Pf/Pan™ were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pf™, Malaria Antigen Pf/Pan™, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, Malaria Antigen Pf/Pan™, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.

Citations

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Multiplexed quantitative proteomics provides mechanistic cues for malaria severity and complexity
Vipin Kumar, Sandipan Ray, Shalini Aggarwal, Deeptarup Biswas, Manali Jadhav, Radha Yadav, Sanjeev V. Sabnis, Soumaditya Banerjee, Arunansu Talukdar, Sanjay K. Kochar, Suvin Shetty, Kunal Sehgal, Swati Patankar, Sanjeeva Srivastava
Communications Biology.2020;[Epub] CrossRef
DIAGNOSTIC PERFORMANCE EVALUATION OF THE SD BIOLINE MALARIA ANTIGEN AG PF/PAN TEST (05FK60) IN A MALARIA ENDEMIC AREA OF SOUTHERN ETHIOPIA
Endale TADESSE, Bereket WORKALEMAHU, Techalew SHIMELIS
Revista do Instituto de Medicina Tropical de São Paulo.2016;[Epub] CrossRef
Epidemiological aspects of vivax and falciparum malaria: global spectrum
Shyamapada Mandal
Asian Pacific Journal of Tropical Disease.2014; 4: S13. CrossRef
Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand
Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang
The Korean Journal of Parasitology.2014; 52(4): 377. CrossRef
Rapid diagnostic tests for diagnosing uncomplicated non-falciparum or Plasmodium vivax malaria in endemic countries
Katharine Abba, Amanda J Kirkham, Piero L Olliaro, Jonathan J Deeks, Sarah Donegan, Paul Garner, Yemisi Takwoingi
Cochrane Database of Systematic Reviews.2014;[Epub] CrossRef