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"sequence analysis"

Brief Communication

Sequence Analysis of cytb Gene in Echinococcus granulosus from Western China
Xiuqin Zhong, Ning Wang, Dandan Hu, Jiahai Wang, Tianyu Liu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
Korean J Parasitol 2014;52(2):205-209.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.205

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance in China. Our main
objective
was to discuss the genotypes and genetic diversity of E. granulosus present in domestic animals and humans in western China. A total of 45 hydatid cyst samples were collected from sheep, humans, and a yak and subjected to an analysis of the sequences of mitochondrial cytochrome b (cytb) gene. The amplified PCR product for all samples was a 1,068 bp band. The phylogenetic analysis showed that all 45 samples were identified as E. granulosus (genotype G1). Ten haplotypes were detected among the samples, with the main haplotype being H1. The haplotype diversity was 0.626, while the nucleotide diversity was 0.001. These results suggested that genetic diversity was low among our samples collected from the west of China based on cytb gene analysis. These findings may provide more information on molecular characteristics of E. granulosus from this Chinese region.

Citations

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  • Sequence analysis of the cytb gene of Mesocestoides Vaillant, 1863 tetrathyridia from small mammals of the Russian Far East
    N.A. Pospekhova, V.V. Pereverzeva, N.E. Dokuchaev, A.A. Primak
    Proceedings of the Zoological Institute RAS.2025; 329(1): 64.     CrossRef
  • G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq
    Musafer H. Al-Ardi
    Journal of Veterinary Science.2024;[Epub]     CrossRef
  • Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes – First report from Sialkot District of Pakistan
    Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Li Li, Muhammad Saqib, Warda Qamar, Ali Hassan, Muzafar Ghafoor, Siddiq Ur Rahman, Muhammad Umar Zafar Khan, Bao-Quan Fu, Youyu Liu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
    Molecular and Biochemical Parasitology.2023; 253: 111542.     CrossRef
  • Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
    Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
    Pathogens.2023; 12(1): 134.     CrossRef
  • Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
    Nigus Abebe Shumuye, Li Li, John Asekhaen Ohiolei, Sayed Ajmal Qurishi, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Sheng-Zhi Gao, Fu-Heng Zhang, Xue-Qi Tian, Wen-Jun Tian, Yong Fu, Xie-Zhong Wang, Yong-Hong Pan, Fang Zhan, Lin-Sheng Zhang, Min
    Parasitology Research.2023; 122(5): 1107.     CrossRef
  • GENETIC ANALYSIS OF SUMATRAN ELEPHANTS IN SEBLAT NATURAL ECOTOURISM PARK BASED ON PARTIAL OF MITOCHONDRIAL CYTOCHROME B GENE
    SIPRIYADI, YANSEN, ARDEA BUJANA, ENI SURYANTI, MUHAMMAD CAHYADI, RICHI YULIAVIAN KUSMINANTO, CHOIRUL MUSLIM, TETI NAIBAHO, MONICA ANGGRAINI
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    Mughees Aizaz Alvi, Abdullah F. Alsayeqh
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Haplotype comparisons of Echinococcus granulosus sensu lato via mitochondrial gene sequences (co1, cytb, nadh1) among Pakistan and its neighbouring countries
    Aisha Khan, Haroon Ahmed, Sami Simsek, Khuram Shahzad, Figen Celik, Muhammad Sohail Afzal, Mobushir Riaz Khan, Hua Liu, Yujuan Shen, Jianping Cao
    Parasitology.2021; 148(9): 1019.     CrossRef
  • Genotypes of Echinococcus isolated from domestic livestock in Kazakhstan
    A.M. Abdybekova, Z. Zhang, A.A. Sultanov, A.A. Abdibayeva, A.A. Zhaksylykova, S.M. Junisbayeva, M.Zh. Aubakirov, G.D. Akhmetova, P.R. Torgerson
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Molecular characterization of human echinococcosis in Sichuan, Western China
    Jingye Shang, Guangjia Zhang, Wenjie Yu, Wei He, Qian Wang, Bo Zhong, Qi Wang, Sha Liao, ruirui Li, Fan Chen, Yan Huang
    Acta Tropica.2019; 190: 45.     CrossRef
  • Genetic characterization ofEchinococcusisolates from various intermediate hosts in the Qinghai-Tibetan Plateau Area, China
    Xiumin Han, Yingna Jian, Xueyong Zhang, Liqing Ma, Wenjun Zhu, Qigang Cai, Shile Wu, Xiangqian Wang, Bingqiang Shi
    Parasitology.2019; 146(10): 1305.     CrossRef
  • Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA
    John Asekhaen Ohiolei, Chen-Yang Xia, Li Li, Jian-Zhi Liu, Wen-Qiang Tang, Yan-Tao Wu, Danqulamu, Guo-Qiang Zhu, Bin Shi, Bao-Quan Fu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau
    Yantao Wu, Li Li, Guoqiang Zhu, Wenhui Li, Nianzhang Zhang, Shuangnan Li, Gang Yao, Wenjun Tian, Baoquan Fu, Hong Yin, Xingquan Zhu, Hongbin Yan, Wanzhong Jia
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Genetic diversity of three Chinese native sheep breeds
    G. -X. E, Y. -Fu. Huang, Y. -Ju Zhao, J. -N. He
    Russian Journal of Genetics.2017; 53(1): 118.     CrossRef
  • Cytochrome b conservation between six camel breeds reared in Egypt
    Othman E. Othman, Heba A.M. Abd El-Kader, Sally S. Alam, Sekena H. Abd El-Aziem
    Journal of Genetic Engineering and Biotechnology.2017; 15(1): 1.     CrossRef
  • Microdiversity of Echinococcus granulosus sensu stricto in Australia
    C. A. ALVAREZ ROJAS, D. EBI, C. G. Gauci, J. P. SCHEERLINCK, M. WASSERMANN, D. J. JENKINS, M. W. LIGHTOWLERS, T. ROMIG
    Parasitology.2016; 143(8): 1026.     CrossRef
  • Surveillance of Echinococcus isolates from Qinghai, China
    Junying Ma, Hu Wang, Gonghua Lin, Fang Zhao, Chao Li, Tongzuo Zhang, Xiao Ma, Yongguo Zhang, Zhibin Hou, Huixia Cai, Peiyun Liu, Yongshun Wang
    Veterinary Parasitology.2015; 207(1-2): 44.     CrossRef
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Original Articles
Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu
Korean J Parasitol 2006;44(4):313-320.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.313

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.

Citations

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  • Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation
    Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu
    The Korean Journal of Parasitology.2022; 60(4): 229.     CrossRef
  • Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review
    Jinfang Wu, Huatao Xie
    Journal of International Medical Research.2021;[Epub]     CrossRef
  • Infectious keratitis and orthokeratology lens use: a systematic review
    Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L. Young
    Infection.2017; 45(6): 727.     CrossRef
  • The Role of Ultraviolet Radiation in the Ocular System of Mammals
    Mercede Majdi, Behrad Milani, Asadolah Movahedan, Lisa Wasielewski, Ali Djalilian
    Photonics.2014; 1(4): 347.     CrossRef
  • Orthokeratology lens related infections
    Kelvin Ho-Nam Wan
    World Journal of Ophthalmology.2014; 4(3): 63.     CrossRef
  • Riboflavin and Ultraviolet Light A Therapy as an Adjuvant Treatment for Medically Refractive Acanthamoeba Keratitis
    Yasin A. Khan, Renata T. Kashiwabuchi, Suy Anne Martins, Juan M. Castro-Combs, Sachin Kalyani, Philip Stanley, David Flikier, Ashley Behrens
    Ophthalmology.2011; 118(2): 324.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2010; 29(6): 680.     CrossRef
  • Twenty Years of Acanthamoeba Keratitis
    F R S Carvalho, A S Foronda, M J Mannis, A L Höfling-Lima, R Belfort, Denise de Freitas
    Cornea.2009; 28(5): 516.     CrossRef
  • Molecular Phylogeny of Acanthamoeba
    Hyun Hee Kong
    The Korean Journal of Parasitology.2009; 47(Suppl): S21.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2009; 28(3): 348.     CrossRef
  • Trends in Microbial Keratitis Associated With Orthokeratology
    Kathleen G. Watt, Helen A. Swarbrick
    Eye & Contact Lens: Science & Clinical Practice.2007; 33(6): 373.     CrossRef
  • 14,058 View
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Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
Korean J Parasitol 2005;43(1):7-13.
Published online March 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.1.7

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.

Citations

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  • The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
    Paul A. Fuerst
    Microorganisms.2023; 11(2): 295.     CrossRef
  • On the diversity and clinical importance of Acanthamoeba spp. from Group 1
    Daniele Corsaro
    Parasitology Research.2021; 120(6): 2057.     CrossRef
  • Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
    Stas Malavin, Lyubov Shmakova
    European Journal of Protistology.2020; 73: 125671.     CrossRef
  • Update on Acanthamoeba phylogeny
    Daniele Corsaro
    Parasitology Research.2020; 119(10): 3327.     CrossRef
  • Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
    Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
    The Korean Journal of Parasitology.2013; 51(4): 401.     CrossRef
  • Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia
    Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed
    Acta Tropica.2011; 117(1): 23.     CrossRef
  • Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
    Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
    The Korean Journal of Parasitology.2008; 46(3): 157.     CrossRef
  • Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a non-contact-lens wearer in Turkey
    Hatice Ertabaklar, Meral Türk, Volkan Dayanir, Sema Ertuğ, Julia Walochnik
    Parasitology Research.2007; 100(2): 241.     CrossRef
  • 8,769 View
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Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
Korean J Parasitol 2004;42(3):129-135.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.129

We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.

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    Parasitology Research.2023; 122(9): 2119.     CrossRef
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    Chang Seok Oh, Jong Ha Hong, Jong Yil Chai, Mi Kyung Song, Ho-Jin Jang, Min Seo, Dong Hoon Shin
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    Journal of Parasitology.2020;[Epub]     CrossRef
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