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"western blotting"

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"western blotting"

Original Articles

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock
Korean J Parasitol 2011;49(2):139-144.
Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.139

The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence of IgG and IgE Antibodies Against Anisakis in the Presumably Healthy Population of the Canary Islands
    Eligia González-Rodríguez, Marta Rodero, J. Alberto Montoya-Alonso, Kevin M. Santana-Hernández, Myriam R. Ventura, Carmen Cuéllar, Eligia Rodríguez-Ponce
    Antibodies.2025; 14(3): 60.     CrossRef
  • Proteome identification of common immunological proteins of two nematode parasites
    Shin Ae Kang, Hak Sun Yu
    Parasites, Hosts and Diseases.2024; 62(3): 342.     CrossRef
  • Progress in Anisakis Allergy Research: Milestones and Reversals
    Alvaro Daschner, Carmen Cuéllar
    Current Treatment Options in Allergy.2020; 7(4): 457.     CrossRef
  • IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis
    S. Mattiucci, A. Colantoni, B. Crisafi, F. Mori‐Ubaldini, L. Caponi, P. Fazii, G. Nascetti, F. Bruschi
    Parasite Immunology.2017;[Epub]     CrossRef
  • Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana
    Rong-Jyh Lin, Mei-Hsuan Wu, Yi-Hsuan Ma, Li-Yu Chung, Chung-Yi Chen, Chuan-Min Yen
    International Journal of Molecular Sciences.2014; 15(3): 3624.     CrossRef
  • Endoscopic imaging of parasites in the human digestive tract
    Naoki Hosoe, Haruhiko Ogata, Toshifumi Hibi
    Parasitology International.2014; 63(1): 216.     CrossRef
  • An Extended Study of Seroprevalence of Anti‐Anisakis simplex IgE Antibodies in Norwegian Blood Donors
    A. H. Lin, I. Nepstad, E. Florvaag, E. Egaas, T. Van Do
    Scandinavian Journal of Immunology.2014; 79(1): 61.     CrossRef
  • Occurrence of anisakid nematode larvae in chub mackerel (Scomber japonicus) caught off Korea
    Tae-Jong Bak, Chan-Hyeok Jeon, Jeong-Ho Kim
    International Journal of Food Microbiology.2014; 191: 149.     CrossRef
  • Clinical Characteristics of Gastroallergic Anisakiasis and Diagnostic Implications of Immunologic Tests
    Young-Bae Chung, Jaechun Lee
    Allergy, Asthma & Immunology Research.2014; 6(3): 228.     CrossRef
  • Preventive and Therapeutic Effects of <italic>Anisakis simplex</italic> Larval Protein in a Mouse Model of Crohn’S Disease
    Hee-Jae Cha, Mee Sun Ock
    Kosin Medical Journal.2013; 28(2): 107.     CrossRef
  • Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niter?i, Brazil
    Israel Junior, Mauricio Vericimo, Luciana Cardoso, Sergio Clemente, Elmiro Nascimento, Gerlinde Teixeira
    Acta Parasitologica.2013;[Epub]     CrossRef
  • Detection of Microorganisms in Granulomas That Have Been Formalin-Fixed: Review of the Literature Regarding Use of Molecular Methods
    Jeannette Guarner
    Scientifica.2012; 2012: 1.     CrossRef
  • Anisakidosis in Korea; Changes Over the Last Decade
    Hee Jae Cha, Mee Sun Ock
    Kosin Medical Journal.2012; 27(2): 73.     CrossRef
  • Prevalence of Zoonotic Anisakid Nematodes in Inuit-Harvested Fish and Mammals from the Eastern Canadian Arctic
    Erica L. Pufall, Andria Jones-Bitton, Scott A. McEwen, Tanya M. Brown, Victoria L. Edge, Jerzy Rokicki, Katarzyna Karpiej, Andrew S. Peregrine, Manon Simard
    Foodborne Pathogens and Disease.2012; 9(11): 1002.     CrossRef
  • A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice
    Israel Figueiredo, Luciana Cardoso, Gerlinde Teixeira, Leila Lopes, Sergio Carmona São Clemente, Mauricio Afonso Vericimo
    Experimental Parasitology.2012; 130(3): 285.     CrossRef
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Identification of newly isolated Babesia parasites from cattle in Korea by using the Bo-RBC-SCID mice
Shin-Hyeong Cho, Tong-Soo Kim, Hyeong-Woo Lee, Masayoshi Tsuji, Chiaki Ishihara, Jong-Taek Kim, Sung-Hwan Wee, Chung-Gil Lee
Korean J Parasitol 2002;40(1):33-40.
Published online March 31, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.1.33

Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea, and Babesia parasites were propagated in SCID mice with circulating bovine red blood cells for isolation. The isolate was then antigenically and genotypically compared with several Japanese isolates. The Korean parasite was found to be nearly identical to the Oshima strain isolated from Japanese cattle, which was recently designated as Babesia ovata oshimensis n. var. Haemaphysalis longicornis was the most probable tick species that transmited the parasite.

Citations

Citations to this article as recorded by  Crossref logo
  • Analysis of spatial and seasonal variations of Haemaphysalis longicornis population based on field survey collected under different habitats and years
    Sunhee Yoon, Jae‐Min Jung, Sumin Oh, Jongmin Bae, Hye‐Min Byun, Subin Choi, Geunho Jang, Minjoon Kang, Eunji Kim, Jaekook Park, Keon Mook Seong, Wang‐Hee Lee, Sunghoon Jung
    Entomological Research.2024;[Epub]     CrossRef
  • Molecular investigation of tick-borne pathogens in ticks removed from tick-bitten humans in the southwestern region of the Republic of Korea
    Mi Seon Bang, Choon-Mee Kim, Sang-Hyun Pyun, Dong-Min Kim, Na Ra Yun, Martin Chtolongo Simuunza
    PLOS ONE.2021; 16(6): e0252992.     CrossRef
  • Current Status of Tick-Borne Diseases in South Korea
    Jae Hyoung Im, JiHyeon Baek, Areum Durey, Hea Yoon Kwon, Moon-Hyun Chung, Jin-Soo Lee
    Vector-Borne and Zoonotic Diseases.2019; 19(4): 225.     CrossRef
  • Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka
    Thillaiampalam Sivakumar, Bumduuren Tuvshintulga, Atambekova Zhyldyz, Hemal Kothalawala, Palitha Rohana Yapa, Ratnam Kanagaratnam, Singarayar Caniciyas Vimalakumar, Thuduwege Sanath Abeysekera, Amitha Sampath Weerasingha, Junya Yamagishi, Ikuo Igarashi, S
    Journal of Clinical Microbiology.2018;[Epub]     CrossRef
  • Cryopreservation of Kudoa septempunctata sporoplasm using commercial freezing media
    Takahiro Ohnishi, Marina Fujiwara, Akiko Tomaru, Tomoya Yoshinari, Yoshiko Sugita-Konishi
    Parasitology Research.2017; 116(1): 425.     CrossRef
  • Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens
    Junya Yamagishi, Masahito Asada, Hassan Hakimi, Takeshi Q. Tanaka, Chihiro Sugimoto, Shin-ichiro Kawazu
    BMC Genomics.2017;[Epub]     CrossRef
  • Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media
    Kodai Kusakisako, Tatsunori Masatani, Yurika Yada, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Hiroki Maeda, Masami Mochizuki, Tetsuya Tanaka
    Parasitology International.2016; 65(5): 532.     CrossRef
  • Babesia ovata: Taxonomy, phylogeny and epidemiology
    Thillaiampalam Sivakumar, Ikuo Igarashi, Naoaki Yokoyama
    Veterinary Parasitology.2016; 229: 99.     CrossRef
  • Human Babesiosis in Europe: what clinicians need to know
    A. Hildebrandt, J. S. Gray, K.-P. Hunfeld
    Infection.2013; 41(6): 1057.     CrossRef
  • Development and biological characteristics of Haemaphysalis longicornis (Acari: Ixodidae) under field conditions
    Hongyuan Zheng, Zhijun Yu, Ze Chen, Lifeng Zhou, Bin Zheng, Hui Ma, Jingze Liu
    Experimental and Applied Acarology.2011; 53(4): 377.     CrossRef
  • Antigenic diversity ofTheileriamajor piroplasm surface protein gene in Jeju black cattle
    Myung-Soon Ko, Kyoung-Kap Lee, Kyu-Kye Hwang, Byung-Sun Kim, Gui-Cheol Choi, Young-Min Yun
    Journal of Veterinary Science.2008; 9(2): 155.     CrossRef
  • First Case of Human Babesiosis in Korea: Detection and Characterization of a Novel Type of Babesia sp. (KO1) Similar to Ovine Babesia
    Jung-Yeon Kim, Shin-Hyeong Cho, Hyun-Na Joo, Masayoshi Tsuji, Sung-Ran Cho, Il-Joong Park, Gyung-Tae Chung, Jung-Won Ju, Hyeng-Il Cheun, Hyeong-Woo Lee, Young-Hee Lee, Tong-Soo Kim
    Journal of Clinical Microbiology.2007; 45(6): 2084.     CrossRef
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Brief Communication
Enzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea
Sung-Hwan Wee, Chung-Gil Lee, Hoo-Don Joo, Yung-Bai Kang
Korean J Parasitol 2001;39(3):261-264.
Published online September 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.3.261

Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspected while one was positive by ELISA. But western blot analyses employed for further confirmation have shown that all of 10 samples did not react to larval excretory-secretory product antigens. These results indicate that pig breeding farms included in the present study are free from trichinellosis. However, it does not mean Korea is free from trichinellosis since human trichinellosis has recently been reported. The necessity of continued surveillance for trichinellosis in both pigs and wild animals was discussed.

Citations

Citations to this article as recorded by  Crossref logo
  • Prevalence of Trichinella spp. antibodies in wild boars (Sus scrofa) and domestic pigs in Korea
    H.J. Kim, W.S. Jeong, E.M. Kim, S.G. Yeo, D.J. An, H. Yoon, E.J. Kim, C.K. Park
    Veterinární medicína.2015; 60(4): 181.     CrossRef
  • Primary characterization and assessment of a T. spiralis antigen for the detection of Trichinella infection in pigs
    Aleksandar Zocevic, Sandrine A. Lacour, Pauline Mace, Baldissera Giovani, Aurelie Grasset-Chevillot, Isabelle Vallee, Pascal Boireau
    Veterinary Parasitology.2014; 205(3-4): 558.     CrossRef
  • Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)
    Leigh Cuttell, Maria Angeles Gómez-Morales, Beth Cookson, Peter J. Adams, Simon A. Reid, Paul B. Vanderlinde, Louise A. Jackson, C. Gray, Rebecca J. Traub
    Veterinary Parasitology.2014; 199(3-4): 179.     CrossRef
  • Seroprevalence of trichinellosis in domestic animals in northwestern Vietnam
    N. Vu Thi, N.V. De, N. Praet, L. Claes, S. Gabriël, P. Dorny
    Veterinary Parasitology.2013; 193(1-3): 200.     CrossRef
  • Development and evaluation of an immunochromatographic strip for trichinellosis detection
    Gai-Ping Zhang, Junq-Qing Guo, Xuan-Nian Wang, Jun-Xing Yang, Yan-Yan Yang, Qing-Mei Li, Xue-Wu Li, Rui-Guang Deng, Zhi-Jun Xiao, Ji-Fei Yang, Guang-Xu Xing, Dong Zhao
    Veterinary Parasitology.2006; 137(3-4): 286.     CrossRef
  • 7,032 View
  • 84 Download
  • Crossref