Immunological responses by soluble egg antigen of <i>Schistosoma mansoni</i> in mice

, Ahn, Myoung Hee; , Colley, Daniel G

doi:1984.22.2.203

Korean J Parasito > Volume 22(2):1984 > Article

Original Article


Korean J Parasitol. 1984 Dec;22(2):203-208. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1984.22.2.203
Copyright © 1984 by The Korean Society for Parasitology


Immunological responses by soluble egg antigen of Schistosoma mansoni in mice
Myoung-Hee Ahn and Daniel G. Colley^*
Department of Parasitology, College of Medicine, Hanyang University, Korea.
^*Department of Microbiology, School of Medicine, Vanderbilt University, USA.


Abstract
This experiment shows cellular and humoral immune responses induced by soluble egg antigen of Schistosoma mansoni, that is, change of the number of peripheral blood eosinophil, delayed hypersensitivity measured by the degree of ear swelling, granulomatous change of liver tissue and elevation of serum antibody titer by ELISA. SEA was given continuously by the insertion of a mini-pump into peritoneal cavity of mouse. In control group, same pump with HGG was inserted. New pump was exchanged once in two weeks and followed the result until 9 weeks after mini-pump insertion. Highest peripheral blood eosinophil level was recorded at 2-3 weeks after SEA pump insertion. Maximum ear swelling was observed at 2 weeks and then decreased gradually. In liver tissue, several granulomas without egg were formed at 4 weeks. Serum antibody titer was elevated from 4 weeks after SEA pump insertion.

Figures


	Fig. 1 The degree of ear swelling of SEA sensitized mouse.


	Fig. 2 A portion of granuloma at 4 weeks after SEA mini-pump insertion. Left side shows necrotic liver tissue and a band of mononu-clear cells and fibrous tissue (H-E, ×400).


	Fig. 3 Serum antibody titer of mouse sensitized with soluble egg antigen or human gamma globulin by ELISA.

Tables


	Table 1 Peripheral blood eosinophil levels during peritoneal administration of SEA or HGG


	Table 2 Serum antibody titers of SEA sensitized mouse to various kinds of antigen(SEA, SWAP, CAP)


	Table 3 Serum antibody titers of mouse to HGG antigen

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