The wormicidal substance of fresh water fishes on <i>Clonorchis sinensis</i> IV. Preliminary research on the wormicidal substance from mucus of <i>Cyprinus carpio nudus</i>

Original Article


Korean J Parasitol. 1983 Jun;21(1):21-26. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1983.21.1.21
Copyright © 1983 by The Korean Society for Parasitology


The wormicidal substance of fresh water fishes on Clonorchis sinensis IV. Preliminary research on the wormicidal substance from mucus of Cyprinus carpio nudus
Jae Ku Rhee,Pyung Gil Kim,Byeong Kirl Baek,Sang Bork Lee and Byung Zun Ahn
Department of Veterinary Medicine, Jeonbug National University, Korea.
Department of Pharmacy, Chungnam National University, Korea.


Abstract
As a series of studies on the suitability as a second intermediate host of Clonorchis sinensis, the wormicidal effects of mucus from epidermis of Cyprinus carpio nudus were observed. The results obtained were summarized as follows: On the silica gel thin layer chromatography with the greenish yellow supernatant in acetone:benzene/10:90 as carrier, seven spots were observed in iodine chamber. The spot of Rf. 0.225 value among them disclosed the strongest wormicidal effect on the cercaria, the excysted metacercaria, and the adult of C. sinensis. Meanwhile, no seasonal variations were found in Rf. values and effect of the wormicidal fractions. The wormicidal materials in epidermal mucus of C. carpio nudus and Carassius carassius were compared in their effect, and the former was more effective killing substance than the latter. It seemed that C. carpio nudus could not be proper intermediate host of C. sinensis according to results of the present study, in addition to our previous observation results.

Figures


	Fig. 1 Fractionation method of wormicidal substance on Clonorchis sinensis from Cyprinus carpio nudus. ^*:Wormicidal activity

Tables


	Table 1 Wormicidal effects of ether extract of mucus from Cyprinus carpio nudus on the cercariae of Clonorchis sinensis


	Table 2 Wormicidal effects of ether extract of mucus from Cyprinus carpio nudus on the excysted metacercariae of Clonorchis sinensis


	Table 3 Wormicidal effects of ether extract of mucus from Cyprinus carpio nudus on the adults of Clonorchis sinensis


	Table 4 Seasonal variation of the wormicidal substances from mucus of Cyprinus carpio nudus on Clonorchis sinensis


	Table 5 Seasonal changes of Rf. value of spots on the TLC of the wormicidal substances from mucus of Cyprinus carpio nudus


	Table 6 Comparison between the time for wormicide of the sixth fraction in Cyprinus carpio nudus and the first fraction in Carassius carassius on the Clonorchis sinensis

References


1.	Pfeiffer W. Experimentia 1963;19(3):113–123.

2.	Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: I Preliminary Research On The Wormicidal Substance From Mucous Substance Of Carassius Carassius]. Korean J Parasitol 1979;17(2):121–126.

3.	Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes]. Korean J Parasitol 1980;18(1):98–104.

4.	Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: III. Seasonal variation on the wormicidal substances of mucous substances of Carassius carassius]. Korean J Parasitol 1980;18(2):179–184.

5.	Rhee JK, Kim PG, Baek BK, Lee SB, Ahn BZ. Clavate Cells Of Epidermis In Cyprinus Carpio Nudus With Reference To Its Defence Activity To Clonorchis Sinensis. Korean J Parasitol 1982;20(2):201–203.

6.	Rhee JK, Kim PG, Baek BK, Lee SB, Ahn BZ. [Experimental Infection Of Clonorchis Sinensis To Cyprinus Carpio Nudus]. Korean J Parasitol 1983;21(1):11–19.