Seropositivity and Serointensity of <italic>Toxoplasma gondii</italic> Antibodies and DNA among Patients with Schizophrenia

Ainsah Omar; Osman Che Bakar; Nor Fatini Adam; Hakim Osman; Arina Osman; Ahmad Hatim Suleiman; Mohd Rizal Abdul Manaf; Mohd Ikhsan Selamat

doi:10.3347/kjp.2015.53.1.29

Korean J Parasito > Volume 53(1):2015 > Article

Omar, Bakar, Adam, Osman, Osman, Suleiman, Manaf, and Selamat: Seropositivity and Serointensity of Toxoplasma gondii Antibodies and DNA among Patients with Schizophrenia

Original Article

Korean J Parasitol 2015; 53(1): 29-34.

Published online: February 27, 2015

DOI: https://doi.org/10.3347/kjp.2015.53.1.29

Seropositivity and Serointensity of Toxoplasma gondii Antibodies and DNA among Patients with Schizophrenia

Ainsah Omar^1,^*, Osman Che Bakar², Nor Fatini Adam², Hakim Osman¹, Arina Osman³, Ahmad Hatim Suleiman⁴, Mohd Rizal Abdul Manaf⁵, Mohd Ikhsan Selamat²

¹Faculty of Medicine and Defence Health, National University of Defence, Malaysia

²Faculty of Medicine, University Teknologi MARA, Sungai Buloh, Selangor, Malaysia

³Faculty of Medicine, Management Science University, Shah Alam, Selangor, Malaysia

⁴Faculty of Medicine, University Malaya Medical Centre, Kuala Lumpur, Malaysia

⁵Department of Community Health, Universiti Kebangsaan Malaysia Medical Center, Kuala Lumpur, Malaysia

^*Corresponding author (oyjaao@yahoo.com.my)

Received October 20, 2014 Revised November 26, 2014 Accepted December 21, 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this cross sectional case control study was to examine the serofrequency and serointensity of Toxoplasma gondii (Tg) IgG, IgM, and DNA among patients with schizophrenia. A total of 101 patients with schizophrenia and 55 healthy controls from Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) were included in this study. The diagnosis of schizophrenia was made based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). The presence of Tg infection was examined using both indirect (ELISA) and direct (quantitative real-time PCR) detection methods by measuring Tg IgG and IgM and DNA, respectively. The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51). However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005). There was no significant difference (P>0.005) in both serointensity of Tg IgG and DNA between patients with schizophrenia and controls. These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

Key words: Toxoplasma gondii, schizophrenia, serofrequency, serointensity

INTRODUCTION

Schizophrenia is a chronic debilitating psychiatric disorder. Despite many studies examining the etiology of schizophrenia the exact cause remains unknown. The evidence of infection as a cause of schizophrenia was supported by the brain histopathological changes found in patients with schizophrenia [1-3]. Studies had documented that astrocytes and neurons of the brain could be infected by Toxoplasma gondii (Tg). The infection then stimulated the production of a variety of cytokines by microglia, astrocytes, and neurons which in turn initiated inflammatory responses [4,5]. The parasites formed cysts within the brain and produce an enzyme called tyrosine hydroxylase, which was needed for dopamine production [4-6]. Dopamine’s role in schizophrenia is well documented. The tyrosine hydroxylase converted L-Dopa to dopamine. An excess of dopamine as a pathological basis for schizophrenia was substantiated by the fact that antipsychotic drugs decreased the brain dopamine thus reducing the symptoms of schizophrenia [7-9]. In addition, antipsychotics inhibited the replication of Tg [10,11]. Tg infection of the brain increased levels of dopamine [12-16] and caused psychotic symptoms resembling schizophrenia [17,18]. Artemether, an antiparasitic agent, significantly reduced negative symptoms of schizophrenia compared to controls [19].

Almost all previous studies concerning the association of Tg infection and schizophrenia were based on indirect measurement by examining antibodies, i.e., IgG and IgM [20-23], and the results were inconclusive [24]. Whilst some studies [24-27] demonstrated positive associations, others found the contrary [28,29]. The purpose of this study was, therefore, to specifically appraise the relationship between schizophrenia and Tg infection using both indirect and direct methods by measuring Tg antibodies and DNA, respectively, in comparison to the controls. The findings would further help in understanding the etiology of schizophrenia which in turn would contribute to the preventive measures and development of a new pharmacological treatment approach for schizophrenia.

MATERIALS AND METHODS

Subjects

This was a cross-sectional case control study examining the serofrequency and serointensity of Tg among patients with schizophrenia and controls, assessed through indirect and direct methods by measuring Tg IgG and IgM antibodies and DNA, respectively. A total of 101 patients with schizophrenia and healthy individuals as controls (n=55) attending Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) who fulfilled the criteria were recruited in this study. Controls were recruited from all consecutive patients attending medical out-patient clinic comprised of patients with chronic hypertension and diabetic with no psychiatry illness. The diagnosis of schizophrenia was made by psychiatrists using the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV), American Psychiatric Association, 1994 [30]. The ethical approvals were obtained from Universiti Teknologi Mara (UiTM), [UiTM 600-RMI(5/1/6)], UMMC (UMMC 932.46), and National Medical Register Research (NMRR), (NMRR-10-852-6764) Ethics Committees. The purpose of the study was explained and a written consent was obtained from patients or the legal guardians. Their demographic data were also recorded.

Detection of Tg IgG, IgM, and DNA

Five ml of blood samples were collected, centrifuged at 1,500 rpm in 15 min at 4˚C, and stored at -20˚C. Tg IgG and IgM antibodies and DNA were measured using ELISA (IBL Company, Hamburg, Germany) and quantitative real-time PCR (qPCR), respectively. The extraction of DNA was performed using QIAamp^® Blood Mini Kit from Qiagen (Hilden, Germany). The tests were all conducted according to the instructions from the manufacturers. Tg IgG and IgM antibodies were measured from serum using the commercial ELISA kit according to the manufacturer’s instructions. Samples absorbance were read using the microtiter plate reader at absorbance of 450/620 nm. Patients and control sera were obtained from blood at the same time as the interviews. Each sample was done triplicate to ensure the reliability of results and the experiments were done in sterile conditions. Positive results were recorded when the quantity of antibodies of IgG and IgM were more than 35 IU/ml and 11 IU/ml, respectively.

The forward primer for qPCR was (5´-TCCCCTCTGCTGGCGAAAAGT-3´), whilst (5´-AGCGTTCGTGGTCAACTATCGATTG-3´) was the reverse primer and (5´6FAM-TCTGTGCAACTTTGGTGTATTCGCA-BHQ1-3´) was the probe primer. A 8-μl of template DNA was added to the final volume of 25 μl reaction mixture, which consists of 6.5 μl of iTAQ Universal Probes Supermix, 0.25 μl (20 μM) Taqman probe, 0.625 μl (20 μM) of each primer, and distilled water. The amplification processes were performed using the Bio-Rad CFX96 machine (Bio-Rad, Hercules, California, USA). The PCR cycling condition was done at 95˚C for 10 min (initial denaturation), followed by 40 cycles at 95˚C for 15 sec (further denaturation), 60˚C for 1 min (annealing), they were then hold for 10˚C. Several sets of PCR amplification using 6 different concentrations of positive samples were performed to calibrate the real-time PCR condition in order to obtain a standard curve with R² more than 85% and Cq standard deviation of less than 0.5. The quantity of the gene was determined using the cycle threshold value (C_T value) by BIORAD CFX Software Version 2.1 (Bio-Rad).

Statistical analysis

The results were analyzed using Statistical Package for Social Sciences (SPSS) version 20 (Chicago, Illinois, USA). The serofrequency of Tg IgG and IgM antibodies were calculated by descriptive statistics (frequencies), and the differences between the groups were calculated using the chi-square or Fisher’s exact test. The differences in medians were compared using the Mann-Whitney U test. The odd ratio (OR) and its 95% Confident Interval (CI) were used to estimate the strength of the association between Tg infection and schizophrenia.

RESULTS

The demographic profiles of the patients with schizopherenia (n=101) and controls (n=55) were comparable in terms of age, gender, and ethnicity (Table 1). The mean ages of controls and patients with schizophrenia were 45.3±14.5 years (range; 21-63 years) and 41.1±10.9 years (18-65 years), respectively. The duration of illness for patients with schizophrenia was 6.5±4.9 years (1.0-13.5 years). Unemployment was significantly (P=0.01) higher among patients with schizophrenia (63.2%) compared to controls (50.9%). The majority of patients with schizophrenia were single (56.1%) compared to controls (27.3%) (P=0.0005). Patients with schizophrenia had a lower level of education (P=0.001) and income (P=0.0005) than controls. The seropositivity and seronegativity of Tg IgG, IgM, and DNA are shown in Table 2.

Tg IgG antibody was positive in 52 (51.5%) and 10 (18.2%) of patients with schizophrenia and controls, respectively (P=0.0005, OD=4.78, 95% CI=2.17-10.51). Meanwhile, the IgM antibody was found in 4 (7.7%) of patients with schizophrenia and none (0%) in controls (OR=3.97, P=0.34, CI= 0.20-78.25). DNA for Tg was positive in 33 (32.67%) of patients with schizophrenia and 2 (3.64%) of controls (P= 0.0005, OD=12.86, CI=2.95-56.03). Table 3 shows the serointensity of Tg IgG, IgM, and DNA among patients and controls. The levels of Tg IgG antibody were not significantly different (P>0.005) in patients with schizophrenia (214.6±163.1 IU/ml) compared to controls (138.4±83.6 IU/ml, P=0.06). There was no significant difference between Tg DNA levels of patients with schizophrenia (0.09 ng/ml) vs controls (0.03± 0.65 ng/ml) (P=0.23). Figs. 1 and 2 show the data of qPCR in controls and patients with schizophrenia, respectively.

DISCUSSION

Our findings showed that there were significant differences between seropositivity of Tg IgG antibody and DNA among patients with schizophrenia compared to controls, suggesting the important role of Tg infection in schizophrenia. These results were consistent with previous studies which found a positive association between Tg IgG antibody and schizophrenia [20,23-25,31,32]. The majority of previous studies determined the presence of Tg infection through an indirect method (using ELISA) by measuring Tg IgG and IgM antibodies. There was, however, no study using real-time qPCR to examine the association between schizophrenia and Tg infection. The strength of this study was the use of both indirect and direct methods for examining the presence of Tg antibodies and DNA, respectively.

The positivity of Tg DNA was significantly higher in patients with schizophrenia than controls with the OR of 12.9. The average OR of previous studies (based on positivity of antibodies) over 5 decades in 17 countries demonstrated an average OR of 2.54 [20,33]. In our study, the OR of having schizophrenia among those with positive Tg DNA (OR=12.9) was much higher than those with positive Tg IgG antibody (OR=4.8). The high OR of Tg infection in schizophrenia measured by Tg DNA in our study indicated that the risk of having schizophrenia among those infected with Tg was much higher than previously considered, signifying that there was a strong relationship between Tg and schizophrenia. The finding also suggested that acute infections of Tg could specifically play an important role in schizophrenia. The detection of Tg infection through direct measurement of DNA was more reliable because it signified the active Tg infection, whereas the seropositivity of Tg IgG would merely indicate the presence of antibodies (as a result of exposure to Tg).

There was no significant difference between Tg IgM antibody in patients with schizophrenia and controls. This finding was consistent with previous studies [34-36]. This could probably be due to the fact that patients often present late to the hospital. The presence of IgM antibodies is time-dependent, and IgM is secreted immediately following infection and would remain positive for 1 or 2 weeks indicating a recent infection. This issue was addressed in this study by examining Tg DNA which reflected the presence of an acute Tg infection.

Although there was significant difference in the seropositivity of Tg IgG and Tg DNA between the 2 groups, however, there was no significant difference between the serointensity of Tg IgG antibody and DNA between schizophrenia patients and controls. These could possibly be due to the effects of antipsychotic medications which caused the reduction in the level of Tg IgG antibody and DNA by inhibiting the replication of Tg [10,11]. The antibody levels for untreated patients with schizophrenia were found to be the highest followed by the treated and controls [34]. The effects of antipsychotic drugs on Tg IgG and DNA levels could be addressed by examining patients with schizophrenia at acute stage, those with the first episode or drug naïve. Secondly, the level of IgG was related to timing of infection and individual body response.

Although our study had strongly demonstrated the association between Tg infection and schizophrenia, a more comprehensive research is required to consolidate these findings. A cohort study involving drug naïve schizophrenia patients would be critical in demonstrating the specificity of the association and would further enlighten the etiological role of Tg in schizophrenia. The clinical factors such as the stages of illness, presentation of symptom, sub-types of schizophrenia as well as treated and non-treated cases should also be considered and addressed.

There were some limitations in this study namely it was a cross-sectional study and there was no comparison to other types of psychosis. A prospective case control study involving a larger sample would be of great help in examining this association. Nevertheless, our study showed that there was a highly significant association between Tg infection and schizophrenia. The strength of the association between Tg infection and schizophrenia was much higher than previously considered. Identifying Tg roles in schizophrenia would help in the preventive measures and pharmacological approaches in the treatment of schizophrenia.

ACKNOWLEDGEMENTS

We would like to express our deepest gratitude to the Director, Head of Department of Psychiatry, staff of Sungai Buloh Hospital, and Head of Department and staff of University Malaya Medical Centre. This work was financially supported by the Fundamental Research Grant Scheme (FRGS), grant no. FRGS/1/10/SP/UiTM/01/9.

Conflict of interest

We have no conflict of interest related to this study.

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Fig. 1.

Quantity of T. gondii DNA in healthy controls by real-time PCR.

Fig. 2.

Quantity of T. gondii DNA in schizophrenia patients by realtime PCR.

Table 1.

Demographic profiles of patients with schizophrenia and control

Demographic profile		Control (n=55)			Schizophrenia (n=101)		Chi square value	P-value
Age	Mean (SD)	45.3 ± 14.5			41.1 ± 10.9		1.86	0.07
	Range	21-63 years			18-65 years
Duration of Illness	Mean (SD)				6.5 ± 4.9
	Range				1-13.5 years

		Control (n=55)			Schizophrenia (n=101)		Chi square value	P-value
		n	(%)		n	(%)	Chi square value	P-value

Occupation	Employed	27		49.1	28	36.8	7.12	0.01
	Unemployed	28		50.9	73	63.2
Gender	Male	24	43.6		58	52.6	2.72	0.10
	Female	31	56.4		43	47.4
Ethnicity	Malay	22	40.0		45	40.4	0.33	0.85
	Chinese	20	36.4		35	42.1
	Indian	13	23.6		21	17.5
Marital status	Married	38	69.1		30	35.1	22.47	0.0005
	Single/Widowed/divorced	17	27.3		71	56.1
Education level	Upper Secondary School and above	39	70.9		44	43.9	10.69	0.001
	Lower Secondary School and below	16	29.1		57	56.1
Family Income per month	< RM2000	25		25.5	92	64.9	39.55	0.0005
	RM2000 and above	30		74.5	9	35.1

RM, Ringgit Malaysia (Malaysian Currency).

Table 2.

Seropositivity and seronegativity of T. gondii IgG and IgM antibodies and DNA among patients with schizophrenia and control

Immunoglobulin and DNA	Control (n=55)	Schizophrenia (n=101)	P-value	Chi-square	OR (95% CI)
IgG positive	10	52	0.0005	16.43	4.78 (2.17-10.51)
IgG negative	45	49
IgM positive	0	4	0.34	0.93	3.97 (0.20-78.25)
IgM negative	55	97
DNA positive	2	33	0.0005	17.25	12.86 (2.95-56.03)
DNA negative	53	68

Table 3.

Serointensity of Toxoplasma gondii IgG and IgM antibodies and DNA among patients with schizophrenia and control

Serointensity of antibodies/DNA	Control (n=55)			Schizophrenia patient (n=101)			P-value	Test value
Serointensity of antibodies/DNA	Median	Range	IQR	Median	Range	IQR	P-value	Test value
IgG IU/ml	138.38	214.58	83.64	214.58	441.53	163.07	0.063	1.86
IgM IU/ml	NA	NA	NA	42.03	71.43	65.18	NA	NA
DNA ng/ml	0.09	0.13	NA	0.30	21.68	0.65	0.227	1.208