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Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica
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Original Article

Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica

I.C. Wang1,3, W.C. Chung2, S.C. Lu3, P.C. Fan1
The Korean Journal of Parasitology 2000;38(4):237-244.
Published online: December 31, 2000

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Corresponding author (pcfan@ym.edu.tw)
• Received: July 3, 2000   • Accepted: September 28, 2000

Copyright © 2000 by The Korean Society for Parasitology

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  • Although oncospheres of Taenia saginata asiatica can develop into cysticerci in immunodeficiency, immunosuppressed, and normal mice, no detailed information on the development features of these cysticerci from SCID mice is available. In the present study, the tumor-like cyst was found in the subcutaneous tissues of each of 10 SCID mice after 38-244 days inoculation with 39,000 oncospheres of T. s. asiatica. These cysts weighed 2.0-9.6 gm and were 1.5-4.3 cm in diameter. The number of cysticerci were collected from these cysts ranged from 125 to 1,794 and the cysticercus recovery rate from 0.3% to 4.6%. All cysticerci were viable with a diameter of 1-6 mm and 9 abnormal ones each with 2 evaginated protoscoleces were also found. The mean length and width of scolex, protoscolex, and bladder were 477 × 558, 756 × 727, and 1,586 × 1,615 µm, respectively. The diameters of suckers and rostellum were 220 µm and 70 µm, respectively. All cysticerci had two rows of rostellar hooks. These findings suggest that the SCID mouse model can be employed as a tool for long-term maintenance of the biological materials for advanced studies of immunodiagnosis, vaccine development, and evaluation of cestocidal drugs which would be most benefit for the good health of the livestocks.
  • Key words: cysticerci, SCID mice, Taenia saginata asiatica
In many countries of East Asia, some people are fond of eating raw or undercooked meat and viscera of domestic and wild animals. These eating habits are important in the transmission of taeniasis (Fan et al., 1992b). Although meat and viscera are commonly eaten and Cysticercus cellulosae is frequently found, but T. saginata rather than T. solium is the dominant species (Cho et al., 1967; Huang et al., 1966; Kosin et al., 1972; Arambulo et al., 1976). In 1992, Eom and Rim proposed this causative agent as a new species of T. asiatica. After considering the closeness of this parasite with the classical T. saginata, we recently proposed this causative agent of taeniasis as a subspecies of T. saginata and named T. s. asiatica. The classical T. saginata was renamed as T. s. saginata (Fan et al., 1995).
Cysticerci of T. s. asiatica have been mostly recovered from the liver of pig, cattle, goat, monkey and wild boar (Fan et al., 1987; Chan et al., 1987). However, pig has been determined to be the favorable intermediate host of this new subspecies (Fan et al., 1990). In addition, some cysticerci in the liver of these intermediate hosts can develop to be degenerated or calcified within one month (Fan et al., 1995). Since this human taeniid cestode has a very high host-specificity, experimental infections can only be repeated in cattle or pigs and it is not easy to obtain information on the biological factors of these tapeworms. Therefore, the establishment of a model with small size animal should be beneficial to the understanding of their developmental biology, immunodiagnosis, pathology, vaccine development, and evaluation of taenicides. Recently, oncospheres of T. s. asiatica and T. solium have been reported to develop into cysticerci in severe combined immunodeficiency (SCID) mice. The mature cysticerci in the subcutaneous tissues of the SCID mice remained viable five months after infection (Ito et al., 1997a, 1997b). Moreover, we have succeeded in establishing a rodent model for study of taeniasis using immunosuppressed or normal mice (Wang et al., 1999a) and infecting hamster with rodent-derived cysticerci to sexual mature worms of T. solium (Wang, 1999b). However, no detailed information on the morphological characteristics of T. s. asiatica cysticerci from SCID mice is available. In the present study, we determined the long period of time for viable cysticerci of T. s. asiatica in SCID mice and also studied the morphological aspects of this metacestode in the rodent model.
Worm collection
Adult worm of T. s. asiatica were collected from infected aborigines at the mountanous areas of Tatung Disrict of Ilan Country, in Taiwan. BALB/c SCID mice were bought from Animal Center, National Taiwan University in Taipei City.
Egg collection and hatching
Eggs of T. s. asiatica were collected from the last ten gravid proglottids of the tapeworm and kept in a refrigerator at 4℃. These eggs were hatched by the enzyme technigue. The detail procedures have been previously described by Wang et al. (1997).
SCID mice as rodent model
Ten SCID mice were inoculated subcutaneously each with 39,000 oncospheres of T. s. asiatica. After experimental infection, the mice were kept in autoclaved (100℃ for 1 h) cages with "Beta-Chip" heat treated hardwood laboratory bedding (Northeaster Products Corp.) and covered with a filter cap. Food and drinking water were also autoclaved and provided ad libitum.
Count and measurement of T. s. asiatica cysticerci
All infected SCID mice were sacrificed after over anaesthesia of ether at various intervals (38-244 days) after infection. Cysticerci from the infected mice were collected, counted, and measured by the method described previously (Fan et al., 1989).
Viable cysticerci recovery and weight and size of the tumor-like cyst
Between 38 and 244 days after inoculationg with 39,000 oncospheres of T. s. asiatica into the subcutaneous tissues of each of 10 SCID mice, a tumor-like cyst was found in the inoculation site of each mouse. The mean weight and diameter of these transparent milky cysts were 4 (range: 1.5-9.6) gm and 3.2 (range: 1.5-4.3) cm, respectively. A total number of cysticerci were 5,899 collected from these cysts ranged from 125 to 1,794 and the mean cysticercus recovery rate of 1.5% and ranged from 0.3% to 4.6%. All cysticerci were viable with a mean diameter of 4 (1-6) mm. Among these cysticerci, 9 were found each with two evaginated protoscoleces in 3 SCID mice (Table 1, Fig. 1-18).
Measurement and counts of evaginated cysticerci of T. s. asiatica
Table 2 shows the measurement and count of cysticerci from 6 infected SCID mice sacrificed from day 62 to day 215 after infection. The mean length and width of scolex, protoscolex, and bladder were 477 (205-1,090) × 558 (280-920), 756 (50-2,950) × 727 (50-2,165), and 1,586 (450-4,775) × 1,615 (425-4,240) µm, respectively. The diameters of suckers and rostellum were 220 (115-315) µm and 70 (30-115) µm, respectively. All the cysticerci had two row of rudimentary hooks. The large inner hooks were 16 (6-25) in number and 9 (3-18) µm long while the outer ones were too small and numerous. There were wart-like formation on the bladder surface (Table 2, Fig. 19-22).
T. solium, T. s. asiatica, and T. s. saginata may employ the pig as an intermediate host. After mature eggs are ingested by the intermediate host, oncospheres hatch from its membrane in the intestinal tract and penetrate the intestinal wall into blood vessels. Through the blood stream, oncospheres of T. solium are carried to various muscles (Beaver et al., 1984) and those of T. s. asiatica and T. s. saginata to the liver (Fan et al., 1992b, 1996). They develop into cysticerci at these sites. Therefore, collection of cysticerci for experimental infection studies require pigs. Fortunately, Ito et al. (1997a) recently succeeded in establishing a SCID mouse model for the development of cysticerci of T. solium and T. s. asiatica. This model has an advantage that the cysticerci of these two parasites remained viable five months after experimental infection. Moreover, we have demonstrated that cysticerci of T. solium and T. s. asiatica not only can develop in immunodeficient mice but also in immmunosuppressed mice and even in normal mice (Wang et al., 1999a). We also found that C57 mice is the most suitable laboratory intermediate host for T. solium among the immunosuppressed mice, since it has a high cysticercus recovery rate of 2.4%. In addition, normal C57 mice can also harbor cysticerci in their subcutaneous tissue, although the cysticercus recovery was relatively low (0.2%). In the establishment and maintenance of T. solium or T. s. asiatica cysticerci in immunosuppressed or normal mice, less efforts and costs are required (Wang et al., 1999a).
In the present study, we found that cysticerci remained viable on day 244 after infection. These cysticerci had a large diameter of 4 (1-6) mm. Moreover, we obtained a high cysticercus recovery rate of 4.6% in a SCID mouse sacrificed on day 62. These findings indicate that the rodent model can be employed not only in the study of the developmental biology, immunodiagnosis, host-parasite relationship, and vaccine development, and of human taeniid cestodes but also can be used as a tool for long-term maintenance of the viable biological materials. In addition, the cysticerci in SCID mice in this study did not become calcified/degeneration after a long period of 244 days. This interval was much longer than that reported by Ito et al. (1997a, 1997b). T. s. asiatica cysticerci recovered from SCID mice in the present study were also much larger than those from pigs (Fan et al., 1995). The increase in size of the cysticerci was found to be proportional to the days of infection. Although Ito et al. (1997a, 1997b) reported that no rostallar hooks were observed on the scolex of cysticerci of T. s. asiatica from SCID mice, we found that there were two rows of rudimentary hooks on the scolex of the cysticerci (Fig. 21). The large inner hooks were 16 (range: 6-25) in mean number and 9 (range: 3-18) µm in length. These findings were similar to the cysticerci obtained from the pig's liver (Fan et al., 1995). It has been suggested that the size of the cysticercus in the intermediate mammalian host might be controlled by some immune response which can not kill the established larvae (Mitchell et al., 1977; Lucas et al., 1980; Ito, 1985; Ishiwata et al., 1992; Dixon and Jenkins, 1995). However, further studies are required to confirm these suggestions. Moreover, in our recent experimental study, we found that the normal and immunosuppressed mice (C3H, C57, and ICR strains) have been demonstrated to be useful as a tool for maintenance of the viable cysticerci of T. solium and T. s. asiatica living longer than one year (Wang et al., unpublished data).
The authors wish to express their sincere thanks to the National Science Council, Republic of China, for support of this research grant (NSC89-2320-B010-039) and a research grant (DOH89-DT-1022) by the Department of Health, Executive Yuan, ROC, and to Miss P. Huang and Miss C.W. Yen for their technical assistance.
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Figs. 1-18
Figs. 1-22. The viable metacestodes of Taenia saginata asiatica were found in big tumor-like cysts in 6 SCID mice 62-215 days after infection each with 39,000 oncospheres. Figs. 1-18. Numerous viable metacestodes were released from the big tumor-like cyst in six SCID mice after sacrifice and decortication.
1. A big tumor-like cyst (9.6 gm and 3 cm in diameter) occupied the left neck, thorax, and both front legs of the SCID mouse (No. 2).
2. Same as Fig. 1. The big tumor-like cyst was exposed after decortication.
3. Same as Fig. 1. Most of 1,794 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.
4. A big tumor-like cyst (3.5 gm and 3 cm in diameter) occupied the left neck, thorax, and both front legs of the SCID mouse (No. 1).
5. Same as Fig. 4. The big tumor-like cyst was exposed after decortication.
6. Same as Fig. 4. A part of 125 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.
7. A big tumor-like cyst (5.0 gm and 3.2 cm in diameter) occupied the right neck thorax, and a front leg of a SCID mouse (No. 4).
8. Same as Fig. 7. One large tumor-like cyst was observed after decortication.
9. Same as Fig. 7. More than half of 529 viable cysticerci and a big mass contained many ones released from the big tumor-like cyst.
10. A big tumor-like cyst (3 gm and 2.5 cm in diameter) occupied the right neck, thorax, and a front leg of a SCID mouse (No. 5).
11. Same as Fig. 10. One big tumor-like cyst was exposed after decortication.
12. Same as Fig. 10. Less than half of 649 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.
13. A large and a small tumor-like cysts (1.5 and 2.5 gm and 1.5 and 2.5 cm in diameter) occupied both left and right thoraces and two front legs of the SCID mouse (No. 6).
14. Same as Fig. 13. A large (right) and a small (left) tumor-like cysts were observed after decortication.
15. Same as Fig. 13. About half of 323 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.
16. A big tumor-like cyst (5.0 gm and 4.3 cm in length) occupied the right thorax, abdomen and two front legs of the SCID mouse (No. 7).
17. Same as Fig. 16. A big tumor-like cyst was exposed after decortication.
18. Same as Fig. 16. About one fourth of 557 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.
Abbreviations: B, bladder; CC, calcareous corpuscles; EVC, evaginated cysticercus; INC, invaginated cysticercus; IH, inner hooks; OH, outer hooks; P, protoscolex; R, rostellum; S, sucker.
kjp-38-237-g001.jpg
Figs. 19-22
Figs. 1-22. The viable metacestodes of Taenia saginata asiatica were found in big tumor-like cysts in 6 SCID mice 62-215 days after infection each with 39,000 oncospheres. Figs. 19-22. Morphological features of normal and abnormal viable evaginated cysticerci and some released ones from two mass cysticerci surrounding with a transparent membrane.
19. A 62 day-old evaginated cysticercus with a rostellum, 4 suckers, a big bladder, and a long protoscolex filled with numerous dark brown calcareous corpuscles (Bar = 100 µm).
20. Three abnormal cysticerci each had two protoscleces (Bar = 100 µm).
21. Enlarged Fig. 19. A rostellum, 2 suckers, and several large inner hooks and numerous small outer hooks (Bar = 30 µm).
22. Two mass of cysticerci surrounding each with a transparent membrane and some releasing invaginated and evaginated ones.
kjp-38-237-g002.jpg
Table 1.
Viable cysticercus recovery and weight and size of the tumor-like cyst in 10 SCID mice each infected with 39,000 oncospheres of Taenia saginata asiatica
Table 1.
Mice No. Age of infection (days) Cysticercus recovery
 Weight (gm)
 Size (in diameter)
No. % Mousea) Tumorb) Tumor (cm) Cysticercus (mm)
1 38 178 0.5 ND ND ND ND
2 62 1,794(3) 4.6 26 9.6 3.0 2(1-3)
3 89 125 0.3 23 3.5 3.0 3(2-4)
4 118 529(3) 1.4 27 5.0 3.2 4(2-5)
5 145 649(3) 1.7 24 3.0 2.5 3(2-5)
6 175 323 0.8 27c) 1.5, 2.5 1.5, 2.5 4(2-5)
7 215 557 1.4 23 5.0 4.3 4(2-6)
8 244 850 2.2 24 4.0 3.5 5(3-6)
9 244 660 1.7 22 3.5 3.0 5(3-6)
10 244 234 0.6 22 2.0 2.0 4(3-6)
Mean 157 590 1.5 24 4 3.2 4
Range 38-244 125-1,794 0.3-4.6 22-27 1.5-9.6 1.5-4.3 1-6

Number of abnormal cysticerci each with two evaginated protoscoleces in parenthesis. ND; Not Done.

a)Measurement before sacrifice.

b)Including the cyst wall, cysticerci, and cyst fluid.

c)With two tumors

Table 2.
Measurement and count of evaginated cysticerci of Taenia saginata asiatica in six SCID mice
Table 2.
  Scolex (μm)
 Protoscolex (μm)
 Bladder (μm)
 Rostellar hook
Days of infection No. exam. Length Width Sucker (μm) (diameter) Rostellum (μm) (diameter) No. of segments Length Width Length Width No. of rows Inner hooksa)
No. Length (μm)
62 100 336 436 199 67 5 307 513 1,044 1,042 2 11 5
210-580 280-585 115-260 48-91 1-11 50-450 50-950 635-1,370 590-1,550 6-16 3-14
89 40 341 411 166 68 5 464 606 950 874 2 16 8
205-490 305-540 120-220 55-90 1-10 105-780 144-950 450-1,430 425-1,550 12-25 4-15
118 50 369 466 213 69 4 358 609 1,494 1,592 2 18 6
240-525 360-590 170-255 30-90 1-12 190-610 118-955 870-2,235 1,055-2,180 12-20 3-12
145 50 538 591 233 76 8 848 870 1,518 1,532 2 16 12
270-1,050 425-865 165-300 45-115 2-20 150-2,900 570-1,360 820-2,300 525-2,180 14-18 8-18
175 50 606 694 252 57 5 785 790 1,724 1,752 2 19 11
400-915 500-920 215-295 35-75 2-9 150-1,550 510-2,165 840-4,240 855-3,900 12-23 3-16
215 50 789 787 265 83 9 2,162 1,165 3,199 3,325 2 16 13
525-1,090 620-915 221-315 41-94 3-18 1,210-2,950 890-1,525 2,630-4,775 2,660-4,240 13-19 5-18
Total 340 477 558 220 70 6 756 727 1,586 1,615 2 16 9
(62-215) 205-1,090 280-920 115-315 30-115 4-9 50-2,950 50-2,165 450-4,775 425-4,240 6-25 3-18

a)Numerous short outer hooks were very difficult to count and measure.

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Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica
Korean J Parasitol. 2000;38(4):237-244.   Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.237
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Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica
Korean J Parasitol. 2000;38(4):237-244.   Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.237
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Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica
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Figs. 1-18 Figs. 1-22. The viable metacestodes of Taenia saginata asiatica were found in big tumor-like cysts in 6 SCID mice 62-215 days after infection each with 39,000 oncospheres. Figs. 1-18. Numerous viable metacestodes were released from the big tumor-like cyst in six SCID mice after sacrifice and decortication.1. A big tumor-like cyst (9.6 gm and 3 cm in diameter) occupied the left neck, thorax, and both front legs of the SCID mouse (No. 2).2. Same as Fig. 1. The big tumor-like cyst was exposed after decortication.3. Same as Fig. 1. Most of 1,794 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.4. A big tumor-like cyst (3.5 gm and 3 cm in diameter) occupied the left neck, thorax, and both front legs of the SCID mouse (No. 1).5. Same as Fig. 4. The big tumor-like cyst was exposed after decortication.6. Same as Fig. 4. A part of 125 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.7. A big tumor-like cyst (5.0 gm and 3.2 cm in diameter) occupied the right neck thorax, and a front leg of a SCID mouse (No. 4).8. Same as Fig. 7. One large tumor-like cyst was observed after decortication.9. Same as Fig. 7. More than half of 529 viable cysticerci and a big mass contained many ones released from the big tumor-like cyst.10. A big tumor-like cyst (3 gm and 2.5 cm in diameter) occupied the right neck, thorax, and a front leg of a SCID mouse (No. 5).11. Same as Fig. 10. One big tumor-like cyst was exposed after decortication.12. Same as Fig. 10. Less than half of 649 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.13. A large and a small tumor-like cysts (1.5 and 2.5 gm and 1.5 and 2.5 cm in diameter) occupied both left and right thoraces and two front legs of the SCID mouse (No. 6).14. Same as Fig. 13. A large (right) and a small (left) tumor-like cysts were observed after decortication.15. Same as Fig. 13. About half of 323 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.16. A big tumor-like cyst (5.0 gm and 4.3 cm in length) occupied the right thorax, abdomen and two front legs of the SCID mouse (No. 7).17. Same as Fig. 16. A big tumor-like cyst was exposed after decortication.18. Same as Fig. 16. About one fourth of 557 viable cysticerci with a few evaginated ones released from the big tumor-like cyst.Abbreviations: B, bladder; CC, calcareous corpuscles; EVC, evaginated cysticercus; INC, invaginated cysticercus; IH, inner hooks; OH, outer hooks; P, protoscolex; R, rostellum; S, sucker.
Figs. 19-22 Figs. 1-22. The viable metacestodes of Taenia saginata asiatica were found in big tumor-like cysts in 6 SCID mice 62-215 days after infection each with 39,000 oncospheres. Figs. 19-22. Morphological features of normal and abnormal viable evaginated cysticerci and some released ones from two mass cysticerci surrounding with a transparent membrane.19. A 62 day-old evaginated cysticercus with a rostellum, 4 suckers, a big bladder, and a long protoscolex filled with numerous dark brown calcareous corpuscles (Bar = 100 µm).20. Three abnormal cysticerci each had two protoscleces (Bar = 100 µm).21. Enlarged Fig. 19. A rostellum, 2 suckers, and several large inner hooks and numerous small outer hooks (Bar = 30 µm).22. Two mass of cysticerci surrounding each with a transparent membrane and some releasing invaginated and evaginated ones.

Figs. 1-18

Figs. 19-22

Rodent model for long-term maintenance and development of the viable cysticerci of Taenia saginata asiatica
Mice No. Age of infection (days) Cysticercus recovery
 Weight (gm)
 Size (in diameter)
No. % Mousea) Tumorb) Tumor (cm) Cysticercus (mm)
1 38 178 0.5 ND ND ND ND
2 62 1,794(3) 4.6 26 9.6 3.0 2(1-3)
3 89 125 0.3 23 3.5 3.0 3(2-4)
4 118 529(3) 1.4 27 5.0 3.2 4(2-5)
5 145 649(3) 1.7 24 3.0 2.5 3(2-5)
6 175 323 0.8 27c) 1.5, 2.5 1.5, 2.5 4(2-5)
7 215 557 1.4 23 5.0 4.3 4(2-6)
8 244 850 2.2 24 4.0 3.5 5(3-6)
9 244 660 1.7 22 3.5 3.0 5(3-6)
10 244 234 0.6 22 2.0 2.0 4(3-6)
Mean 157 590 1.5 24 4 3.2 4
Range 38-244 125-1,794 0.3-4.6 22-27 1.5-9.6 1.5-4.3 1-6
  Scolex (μm)
 Protoscolex (μm)
 Bladder (μm)
 Rostellar hook
Days of infection No. exam. Length Width Sucker (μm) (diameter) Rostellum (μm) (diameter) No. of segments Length Width Length Width No. of rows Inner hooksa)
No. Length (μm)
62 100 336 436 199 67 5 307 513 1,044 1,042 2 11 5
210-580 280-585 115-260 48-91 1-11 50-450 50-950 635-1,370 590-1,550 6-16 3-14
89 40 341 411 166 68 5 464 606 950 874 2 16 8
205-490 305-540 120-220 55-90 1-10 105-780 144-950 450-1,430 425-1,550 12-25 4-15
118 50 369 466 213 69 4 358 609 1,494 1,592 2 18 6
240-525 360-590 170-255 30-90 1-12 190-610 118-955 870-2,235 1,055-2,180 12-20 3-12
145 50 538 591 233 76 8 848 870 1,518 1,532 2 16 12
270-1,050 425-865 165-300 45-115 2-20 150-2,900 570-1,360 820-2,300 525-2,180 14-18 8-18
175 50 606 694 252 57 5 785 790 1,724 1,752 2 19 11
400-915 500-920 215-295 35-75 2-9 150-1,550 510-2,165 840-4,240 855-3,900 12-23 3-16
215 50 789 787 265 83 9 2,162 1,165 3,199 3,325 2 16 13
525-1,090 620-915 221-315 41-94 3-18 1,210-2,950 890-1,525 2,630-4,775 2,660-4,240 13-19 5-18
Total 340 477 558 220 70 6 756 727 1,586 1,615 2 16 9
(62-215) 205-1,090 280-920 115-315 30-115 4-9 50-2,950 50-2,165 450-4,775 425-4,240 6-25 3-18
Table 1. Viable cysticercus recovery and weight and size of the tumor-like cyst in 10 SCID mice each infected with 39,000 oncospheres of Taenia saginata asiatica

Number of abnormal cysticerci each with two evaginated protoscoleces in parenthesis. ND; Not Done.

Measurement before sacrifice.

Including the cyst wall, cysticerci, and cyst fluid.

With two tumors

Table 2. Measurement and count of evaginated cysticerci of Taenia saginata asiatica in six SCID mice

Numerous short outer hooks were very difficult to count and measure.

Table 1.

Table 2.

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