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Intestinal mastocytosis and goblet cell hyperplasia in BALB/c and C3H mice infected with Neodiplostomum seoulense
J Y Chai,*T K Kim,W H Cho,M Seo,J Kook,S M Guk and S H Lee
Department of Parasitology, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received February 25, 1998; Accepted April 14, 1998.
Abstract
Mucosal mast cell (MMC) and goblet cell (GC) responses were observed in the small intestine of two strains of mice (BALB/c and C3H) infected with Neodiplostamum seoulense, and their roles in the host defense and worm expulsion were studied. From day 3 to 28 post-infection (PI) with 200 metacercariae, the worm recovery rate from BALB/c mice was consistently and remarkably higher than that from C3H mice. In the duodenum of both strains of mice, the main habitat of the flukes, mastocytosis was pronounced on day 7 PI but quickly diminished thereafter. Similar kinetics were observed in the jejunum and ileum, although the extent of mastocytosis was lesser in the ileum than other two areas. These MMC kinetics were not different between the two strains of mice. Moreover, the extent of mastocytosis was stronger in BALB/c mice than in C3H mice. GC hyperplasia was remarkable in the duodenum of BALB/c mice throughout the course of infection except day 14 PI, whereas it was recognizable only in the jejunum and ileum of C3H mice on day 7 PI. Mucin activation was evidently demonstrated in both strains of mice throughout the course of infection, but more marked in BALB/c than in C3H mice. The results strongly suggest that mastocytosis and GC hyperplasia are local immune responses against N. seoulense, however, they play a minor role in the host defense and worm expulsion.
Figures
Fig. 1 Comparison of mucosal mast cell (MMC) numbers per 10 villus-crypt unit (VCU) in the small intestine of BBALB/c and C3H mice infected with N. seoulense. Values represent the mean of data from 5 mice each consisting of three samples on each day. Standard deviation rarely exceeded the mean value.
Figs. 2-3 Fig. 2. Mucosal mastocytosis in the duodenum of a BALB/c mouse infected with N. seoulense, day 7 PI. Blue spots represent MMCs. Stained with alcian blue and safranin. ×500. Fig. 3. Decreased number of MMCs to nearly normal level on day 21 PI in the duodenum of a BALB/c mouse infected with N. seoulense. ×500.
Fig. 4 Comparison of goblet cell (GC) numbers per 10 villus-crypt unit (VCU) in the small intestine of BALB/c and C3H mice infected with N. seoulense. Values represent the mean of data from 5 mice each consisting of three samples on each day. Standard deviation never exceeded the mean value.
Figs. 5-6 Fig. 5. The duodenum of a normal BALB/c mouse showing only a small number of GCs (arrows). Stained with PAS, and counterstained with hematoxylin. ×500. Fig. 6. Marked GC hyperplasia in the ileum of a BALB/c mouse infected with N. seoulense, day 28 PI. Dark spots (arrows) represent GCs. ×650.
Fig. 7 Comparison of Helix pomatia agglutinin (HPA) positive cell numbers per 10 villus-crypt unit (VCU) in the small intestine of BALB/c and C3H mice infected with N. seoulense. Values represent the mean of data from 5 mice each consisting of three samples on each day. Standard deviation never exceeded the mean value.
Figs. 8-9 Fig. 8. The duodenum of a normal BALB/c mouse showing only a small number of activated GCs (arrows) in which altered mucins around GCs are stained dark brown. Lectin histochemistry was performed using Helix pomatia agglutinin (HPA) which specifically recognizes the terminal GalNAc residue on altered GC mucins. ×500. Fig. 9. HPA positive GCs (arrows) in the ileum of a BALB/c mouse infected with N. seoulense. day 28 PI. Lectin histochemistry was performed by the same procedure as in Fig. 8. ×650.
Tables
Table 1 Comparison of the WRRs from BALB/c and C3H mice infected with N. seoulense
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