The role of nitric oxide as an effector of macrophage-mediated cytotoxicity against <i>Trichomonas vaginalis</i>

, Park, G C; , Ryu, J S; , Min, D Y

doi:1997.35.3.189

Korean J Parasito > Volume 35(3):1997 > Article

Original Article


Korean J Parasitol. 1997 Sep;35(3):189-195. English. Published online Sep 20, 1997. http://dx.doi.org/10.3347/kjp.1997.35.3.189
Copyright © 1997 by The Korean Society for Parasitology


The role of nitric oxide as an effector of macrophage-mediated cytotoxicity against Trichomonas vaginalis
G C Park,¹J S Ryu,^*² and D Y Min²
Department of Obstetrics and Gynecology, College of Medicine, Yonsei University, Seoul 120-751, Korea.

Received August 09, 1997; Accepted September 05, 1997.
Abstract
The purpose of this study is to determine whether nitric oxide is involved in the extracellular killing of Trichomonas vaginalis by mouse (BALB/c) peritoneal macrophages and RAW264.7 cells activated with LPS or rIFN-γ and also to observe the effects of various chemicals which affect the production of reactive nitrogen intermediates (RNI) in the cytotoxicity against T. vaginalis. The cytotoxicity was measured by counting the release of [³H]-thymidine from labelled protozoa and NO₂- was assayed by Griess reaction. N^G-monomethyl-L-arginine (L-NMMA), N^G-nitro-L-arginine methyl ester (NAME) and arginase inhibited cytotoxicity to T. vaginalis and nitrite production by activated mouse perioneal macrophages and RAW 264.7 cells. The addition of excess L-arginine competitively restored trichomonacidal activity of macrophages. Exogenous addition of FeSO₄ inhibited cytotoxicity to T. vaginalis and nitric products of macrophages. From above results, it is assumed that nitric oxide plays an important role in the host defense mechanism of macrophages against T. vaginalis.

Figures


	Fig. 1 Effects of IFN-γ and/or LPS on the cytotoxicity to T. vaginalis and nitrite production by mouse peritoneal macrophages (MPMs) or RAW264.7 cells. Control groups are MPMs and RAW264.7 cells without IFN-γ or LPS. Results by macrophages activated with IFN-γ and/or LPS, were compared with that of control macrophages. ^*p < 0.005, Mann-Whitney test


	Fig. 2 Effects of L-NMMA, NAME, arginase on the cytotoxicity to T. vaginalis and nitrite production by LPS-activated mouse peritoneal macrophages (MPMs) and IFN-γ-activated RAW264.7 cells. Control macrophages are MPMs and RAW264.7 cells activated with LPS alone and IFN-γalone, respectively. ^*p < 0.05, Mann-Whitney test


	Fig. 3 Effect of H₄B on the cytotoxicity to T. vaginalis and nitrite production by LPS-activated mouse peritoneal macrophages (MPMs) and IFN-γ-activated RAW264.7 cells. Control macrophages are MPMs and RAW264.7 cells activated with LPS alone and IFN-γ alone, respectively. ^*p < 0.05, Mann-Whitney test


	Fig. 4 Effect of FeSO₄ on the cytotoxicity to T. vaginalis and nitrite production by LPS-activated mouse peritoneal macrophages (MPMs) and IFN-γ-activated RAW264.7 cells. Control macrophages are MPMs and RAW264.7 cells activated with LPS alone and IFN-γ alone, respectively. ^*p < 0.05, Mann-Whitney test

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