Development of mass rearing technique of <i>Tyrophagus putrescentiae</i> (Acari: Acaridae) found in house dust

Original Article


Korean J Parasitol. 1997 Sep;35(3):149-154. English. Published online Sep 20, 1997. http://dx.doi.org/10.3347/kjp.1997.35.3.149
Copyright © 1997 by The Korean Society for Parasitology


Development of mass rearing technique of Tyrophagus putrescentiae (Acari: Acaridae) found in house dust
H I Ree,^* and I Y Lee
Department of Parasitology, College of Medicine, Yonsei University, Seoul 120-752, Korea.

Received July 15, 1997; Accepted August 18, 1997.
Abstract
A storage mite, Tyrophagus putrescentiae, is recently known to be widely distributed in Korea, being commonly found in house dust, and may, therefore, be allergenically important. The purpose of this study was to develop mass rearing techniques for supplying a large quantity of allergens. The laboratory mouse food powder gave the highest yield, showing 1,251.5-fold increase in number after 10 weeks, and the mixed powder of laboratory mouse food and yeast (1:1) also gave same level of the production (1,203.1-fold increase in week 10). Several different combinations of temperature and relative humidity conditions were compared, and the maximum propagation was obtained at 25℃ and 64% RH, showing 960-fold increase in number. When the same amount of culture media was used, the size of the culture container did not significantly influence the quantitative yield of T. putrescentiae mites.

Figures


	Fig. 1 Culture method of mites by using large and small containers. A: lid, B: large plastic container (20 cm in diameter), C: saturated solution of NaCl, Na₄HO₃ or NaHSO₄, D: plastic culture container (14 cm in diameter), E: culture medium and mites.

Tables


	Table 1 Comparative mass production of Tyrophagus putrescentiae in the different culture media (50g) nuder the condition of 25℃ and 75% RH


	Table 2 Comparative mass production of Tyrophagus putrescentiae under the different temperature and humidity conditions in 50g of the culture medium (Laboratory mouse food: Yeast, 1:1)


	Table 3 Comparative mass rearing of Tyrophagus putrescentiae in the different sizes of the container in 50g of culture media (Laboratory mouse food: Yeast, 1:1) under the condition of 25℃ and 75% RH

References


1.	Arlian LG, Geis DP, Vyszenski-Moher DL, Bernstein IL, Gallagher JS. Antigenic and allergenic properties of the storage mite Tyrophagus putrescentiae. J Allergy Clin Immunol 1984;74(2):166–171.

2.	Arlian LG, Geis DP, Vyszenski-Moher DL, Bernstein IL, Gallagher JS. Cross antigenic and allergenic properties of the house dust mite Dermatophagoides farinae and the storage mite Tyrophagus putrescentiae. J Allergy Clin Immunol 1984;74(2):172–179.

3.	van Bronswijk JE, Sinha RN. Pyroglyphid mites (Acari) and house dust allergy. J Allergy 1971;47(1):31–52.

4.	Miyamoto J, Ishii A, Sasa M. A successful method for mass culture of the house dust mite, Dermatophagoides pteronyssinus (Troussart, 1897). Jpn J Exp Med 1975;45(2):133–138.

5.	Ree HI, Jeon SH, Lee IY, Hong CS, Lee DK. Fauna and geographical distribution of house dust mites in Korea. Korean J Parasitol 1997;35(1):9–17.

6.	Ree HI, et al. Med Entomol Zool 1997;48(2):109–116.

7.	Sasa M, Miyamoto J, Shinoara S, Suzuki H, Katsuhata A. Studies on mass culture and isolation of Dermatophagoides farinae and some other mites associated with house dust and stored food. Jpn J Exp Med 1970;40(5):367–382.

8.	Solomon MW. Bull Entomol Res 1952;42:543–554.