Survival of <i>Cryptosporidium muris</i> (strain MCR) oocysts under cryopreservation

, Rhee, J K; , Park, B K

doi:1996.34.2.155

Korean J Parasito > Volume 34(2):1996 > Article

Brief Communication


Korean J Parasitol. 1996 Jun;34(2):155-157. English. Published online Jun 20, 1996. http://dx.doi.org/10.3347/kjp.1996.34.2.155
Copyright © 1996 by The Korean Society for Parasitology


Survival of Cryptosporidium muris (strain MCR) oocysts under cryopreservation
J K Rhee,^*¹ and B K Park²
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 561-756, Korea.

Received March 05, 1996; Accepted April 17, 1996.
Abstract
We have successfully maintained Cryptosporidium muris by cryopreservation. Oocysts were suspended in distilled water, stored at - 20℃ for 24 hrs, and then cryopreserved at -70℃. Cryopreserved specimens were slowly thawed at 5℃. Oocysts, which had been cryopreserved for 15 months without cryoprotective agents, retained their infectivity by the mouse titration method. Oocysts stored at 5℃ in 2.5% potassium dichromate failed to retain their infectivity beyond 6.5 months.

References


1.	Dwyer DM, et al. J Parasitol 1971;57(1):190–191.

2.	Moril T, Matsui T, Iijima T, Abe N, Yoshizawa A, Chikatsune M, Watanabe M. Cryopreservation of Leucocytozoon caulleryi sporozoites. J Protozool 1988;35(3):356–359.

3.	Reese NC, Current WL, Ernst JV, Bailey WS. Cryptosporidiosis of man and calf: a case report and results of experimental infections in mice and rats. Am J Trop Med Hyg 1982;31(2):226–229.

4.	Rhee JK. Supplemental knowledge on survival of Thelohanellus kitauei spores in vitro. Korean J Parasitol 1994;32(1):57–59.

5.	Rhee JK, Seu YS, Park BK. [Isolation and identification of Cryptosporidium from various animals in Korea. I. Prevalence of Cryptosporidium in various animals. Korean J Parasitol 1991;29(2):139–148.

6.	Rhee JK, Seu YS, Park BK. [Isolation and identification of Cryptosporidium from various animals in Korea. II. Identification of Cryptosporidium muris from mice]. Korean J Parasitol 1991;29(2):149–159.

7.	Rhee JK, Jang BG, Park BK. Oocyst production and immunogenicity of Cryptosporidium baileyi in chickens and mallards. Korean J Parasitol 1995;33(1):45–54.

8.	Rhee JK, Yook SY, Park BK. Oocyst production and immunogenicity of Cryptosporidium muris (strain MCR) in mice. Korean J Parasitol 1995;33(4):377–382.

9.	Robertson LJ, Campbell AT, Smith HV. Survival of Cryptosporidium parvum oocysts under various environmental pressures. Appl Environ Microbiol 1992;58(11):3494–3500.

*10.*	Rossi P, Pozio E, Besse MG. Cryopreservation of Cryptosporidium sp. oocysts. Trans R Soc Trop Med Hyg 1990;84(1):68.

*11.*	Sherwood D, Angus KW, Snodgrass DR, Tzipori S. Experimental cryptosporidiosis in laboratory mice. Infect Immun 1982;38(2):471–475.