Inhibition of Con A-induced lymphocyte proliferation by peritoneal exudate of <i>Toxoplasma gondii</i>-infected mice

, Nam, H W; , Choi, W Y

doi:1995.33.3.195

Korean J Parasito > Volume 33(3):1995 > Article

Original Article


Korean J Parasitol. 1995 Sep;33(3):195-200. English. Published online Sep 20, 1995. http://dx.doi.org/10.3347/kjp.1995.33.3.195
Copyright © 1995 by The Korean Society for Parasitology


Inhibition of Con A-induced lymphocyte proliferation by peritoneal exudate of Toxoplasma gondii-infected mice
H W Nam,^* and W Y Choi
Department of Parasitology, Catholic University Medical College, Seoul 137-107, Korea.

Received July 21, 1995; Accepted August 11, 1995.

Abstract
The presence of biological response modifiers (BRM)-like effect was confirmed in peritoneal exudate (PE) of Toxoplasma gondii-infected ICR mice which inhibited Concanavalin A (Con A)-induced peritoneal lymphocyte (PL) proliferation. During 5 days of PL incubation with 10 µg/ml Con A with or without PE, ³H-thymidine uptake was measured for the last 24 hrs. Compared to uninduced control, PL proliferated by 7.3-fold with Con A induction. When PE of infected mice was added, PL proliferation was inhibited by 74.0 ± 11.9% whereas inhibition by PE of normal mice was 16.4 ± 8.3%. Inhibitory effect of PE increased exponentially from 3 days up to 4-5 days of survival after the infection. Inhibitory activity of PE was decreased concentration- dependently. Also the inhibition was diminished when the PE was treated with heat of 95℃ for 10 min or precipitated with 10% trichloracetic acid (TCA). In SDS-PAGE of PE, many minor bands appeared newly. Heat-labile protein molecule in PE exerted inhibitory activity to Con A-induced lymphocyte proliferation.

Figures


	Fig. 1 Inhibition of Con A-induced peritoneal lymphocyte (Pl) proliferation by peritoneal exudate of mice infected with T. gondii. Control, PL only; Con A, PL induced with Con A; Con A + PE, PL induced with Con A in the presence of peritoneal exudate; and PE, PL without Con A but with peritoneal exudate.


	Fig. 2 Percent inhibition of Con A-induced proliferation by PE from infected and non-infected mice. Percent inhibition was calculated by the formula: (1-cpm of PL with Con A and PE/ cpm of PL with Con A) ×100. PE (infected), PE from infected mice; and PE (Normal), PE from non-infected mice.


	Fig. 3 Changing pattern of inhibitory effects on Con A-induced PL proliferation in PE which were collected consecutively after peritoneal infection with T. gondii.


	Fig. 4 Effect of PE dilution on inhibition of Con A-induced PL proliferation. PE added to the culture well was 10% (v/v) of media primarily, the dilution factors were further multiplied by 10 actually.


	Fig. 5 SDS-PAGE pattern of PE on the time course of postinfection. M, molecular weight markers; N, PE of uninfected normal mouse, and 1-4, day after infection.


	Fig. 6 Effects heat (95℃ for 10 min) and 10% TCA precipitation on inhibition of Con A-induced PL proliferation.

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