Immunogenicity and protective efficacy of solubilized merozoite-enriched <i>Theileria sergenti</i> immmunogens III. Characterization of immunodominant peptides

, Baek, Byeong Kirl; , Kim, Byeong Soo; , Rhim, Byung Moo; , Lee, Ho Ill; , Park, Yong Ho; , Kakoma, Ibulaimu

doi:1994.32.2.111

Korean J Parasito > Volume 32(2):1994 > Article

Original Article


Korean J Parasitol. 1994 Jun;32(2):111-116. English. Published online Jun 20, 1994. http://dx.doi.org/10.3347/kjp.1994.32.2.111
Copyright © 1994 by The Korean Society for Parasitology


Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immmunogens III. Characterization of immunodominant peptides
Byeong Kirl Baek,^*¹Byeong Soo Kim,¹Byung Moo Rhim,¹Ho Ill Lee,¹Yong Ho Park,² and Ibulaimu Kakoma³
¹Chonbuk National University, College of Veterinary Medicine, chonju 560-756, Korea.
²Veterinary Research Institute, Ahnyang 430-016, Korea.
³University of Illinois, Urbana IL 61801 USA.

Received May 02, 1994; Accepted May 30, 1994.
Abstract
Immunoblot analysis utilizing bovine sera from naturally or experimentally infected with Theileria sergenti were used to determine the immunodominant polypeptides of T. sergenti (Korea isolate).The previously recognized major bands, 18 kDa, 29 kDa, 34 kDa, and 45 kDa, were excised after electrophoresis and trasferred to PVDF membrane. The individual bands were sequenced. The 34 kDa polypeptide which was the most antigenic and immunogenic peptide was observed in the Western blot. However, Chou-Fasman prediction sites (antiginic site) for antigen determinants of the 45 kDa,34 kDa, 29 kDa and 18 kDa polypeptide were 6, 4, 2 and 0, respectively. However, the 45 kDa polypeptide showed no reaction with anti-T. sergenti hyperimmune serum.

Figures


	Fig. 1 The partial NH₂-terminal amino acid sequences of the major merozoite immunogens.

Fig. 2
Two-dimentional graphs of T. sergenti immunodominant peptide using Chou-Fasman parameter. Predicted antigenic sites in plotstructure of amino acid analyzed was sequenced from -NH₂-to-COOH. A. 6 hydrophilic regions (antigenic sites) are observed in 45 kDa major. B. 4 antigenic sites are observed in 34 kDa major. C. 2 antigenic sites are observed in 29 kDa major. D. No hydrophilic regions is observed in 18 kDa major.

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