Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81
Warning: fopen(upload/ip_log/ip_log_2024-11.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. I: Protection against homologous stabilate challenge
Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. I: Protection against homologous stabilate challenge
B K Baek,I H Choi,B S Kim,R Hansen,* and I Kakoma*
College of Veterinary Medicine, Chonbuk National University, Chonju 560-756, Korea.
Abstract
Theileria sergenti were isolated from infected erythrocytes by hypotonic lysis, and soluble merozoite antigens were purified by sonication and differential centrifugation. The preparation contained 29, 34, 35 and 105 kD immuno-dominant poly-peptides. The soluble antigens (0.5 mg/ml) were prepared and fortified with Freund's adjuvant. Five month old naive Korean calves were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later. Nine weeks after the booster dose, vaccinates and controls were challenged with a homologous stabilate (5.6 × 106 RBC/dose, 40% Parasitemia). All animals were monitored for hematocrit, total erythrocyte count, parasitemia and for the specific antibody by Western immunoblot (WB) and indirect immuno-fluorescent antibody (IFA) test. By 18 weeks after vaccination (6 weeks after the challenge), vaccinated cattle had an average IFA titer of 1:10,240 compared with 1:1,280 of the controls. The vaccinates showed negligible change in hematocrit and total RBC count whereas control animals showed significant (p < 0.05) hematological changes and associated anemia. After vaccination and challenge, the antibody responses demonstrated that vaccination had induced significant production of antibody to the 29 and 35 kD polypeptides. The latter polypeptide was much more strongly recognized by the vaccinated animals, and thus it may be a potential candidate for the vaccine.
Figures
Fig. 1 SDS-PAGE results on T. sergenti antigen (Coomassie stain).
Fig. 2 Western immunoblot analysis of T. sergenti antigens.
Key : PO = Positive serum from a cow experimentally infected with T. sergenti., NE = Normal pre-infection serum
Fig. 3 Intensity of immunfluorescence with immune anti-T. sergenti serum (insert shows background for negative bovine serum).
Fig. 4 IFA response in vaccinated(-▪-) and control (-•-) animals.
Fig. 5 Total RBC(-) and HT(--) profile in vaccinated (-▪-) and control (-•-) animals.
References
1.
Asaoka H, Onuma M, Kawamoto S, Takahashi K, Kawakami Y. Activation of bovine peripheral blood macrophages in Theileria sergenti-infected calves. Res Vet Sci 1991;50(1):23–28.
2.
Baek BK, et al. Korean J Vet Res 1990;30:223–229.
3.
Baek BK, et al. Korean J Vet Sci 1990;30:465–471.
4.
Baek BK, et al. Korean J Vet Publ Hlth 1991;15:127–141.
5.
Brown CGD, et al. J Parasitol 1971;57:59–60.
6.
Burridge MJ, Morzaria SP, Cunningham MP, Brown CG. Duration of immunity to East Coast fever (Theileria parva infection of cattle). Parasitology 1972;64(3):511–515.
7.
Jang DH. [Epizootiological study of theileriasis in Korea: Prevalence of the bovine theileriasis in relation to its vector, Haemaphysalis (Kaiseriana) longicornis Neumann 1901]. Korean J Parasitol 1974;12(1):14–20.
8.
Cunningham MP, Brown CG, Burridge MJ, Irvin AD, Kirimi IM, Purnell RE, Radley DE, Wagner GG. Theileriosis: the exposure of immunized cattle in a Theileria lawrencei enzootic area. Trop Anim Health Prod 1974;6(1):39–43.
9.
Codge JT, et al. Arch Biochem Biophys 1983:130.
10.
Fujisaki K, et al. Jpn J Vet Sci 1989;51:457–459.
11.
Han TW. Res Pept RDA 1971;13:97–102.
12.
Hubbert WT, et al. Theriogenology 1975;3:43–63.
13.
Kajiwara N, et al. Jpn J Vet Sci 1990;52:1199–1204.
14.
Kang YB, et al. Res Rept RDA 1988;30:17–21.
15.
Kim IC, et al. Korean J Anim Sci 1983;25:464–459.
16.
Kim IC, et al. Korean J Anim Sci 1984;26:137–144.
17.
Kobayashi N, et al. Jpn J Vet Sci 1987;49:697–702.
18.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
19.
Love JN. Cryogenic preservation of Anaplasma marginale with Dimethyl sulfoxide. Am J Vet Res 1972;33(12):2557–2560.
20.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
21.
Montenegro-James S, et al. Am Vet Res 1985;40:2401–2403.
22.
Mutugi JJ, Young AS, Kariuki DP, Tameno JM, Morzaria SP. Epidemiological observations on theileriosis following field immunisation using infection and treatment. Trop Anim Health Prod 1991;23(2):75–82.
23.
Ohgitani T, et al. Jpn J Vet Sci 1987;49:531–534.
24.
Radley DE, et al. Vet Parasitol 1975;1:35–41.
25.
Radley DE, et al. Vet Parasitol 1975;1:51–60.
26.
Radley DE, et al. Vet Record 1975;14:525–527.
27.
Radley DE, et al. Vet Parasitol 1975;1:43–50.
28.
Robinson PM. Theileriosis annulata and its transmission-a review. Trop Anim Health Prod 1982;14(1):3–12.
29.
Sugimoto C, Kawazu S, Kamio T, Fujisaki K. Protein analysis of Theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis. Parasitology 1991;102(Pt 3):341–346.
30.
Sugimoto C, Sato M, Kawazu S, Kamio T, Fujisaki K. Purification of merozoites of Theileria sergenti from infected bovine erythrocytes. Parasitol Res 1991;77(2):129–131.
31.
Suh MD, et al. Res Pept RDA 1972;14:41–46.
32.
Tanaka M, Ikeda M, Matsuba T, Okabe T, Takahashi K, Onuma M, Sasaki N. Induction of anti-Theileria sergenti antibodies in calves with murine monoclonal anti-idiotype antibody. J Vet Med Sci 1991;53(5):775–778.
33.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 1979;76(9):4350–4354.
34.
Uilenberg G. Science 1981;14:4–37.
35.
Yasutomi Y, Asaoka H, Takahashi K, Kawakami Y, Onuma M. Proliferation of lymphocytes in Theileria sergenti-infected calves in vitro. J Vet Med Sci 1991;53(1):161–162.
36.
Young AS, Brown CG, Burridge MJ, Cunningham MP, Kirimi IM, Irvin AD. Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. Int J Parasitol 1973;3(6):723–728.
PDF Links
Full text via DOI
Download Citation 
