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Study on the therapeutic effects of interferon and gamma-globulin in experimental Pneumocystis carinii pneumonia
Dae-Whan Shin,Dae-Young Kang,*Young-Ha Lee,Young-Eun Na and Keon-Jung Yun
Department of Parasitology, College of Medicine, Chungnam National University, Taejon 301-131, Korea.
*Department of Pathology, College of Medicine, Chungnam National University, Taejon 301-131, Korea.
Abstract
This study was performed to observe the therapeutic effects of interferon-gamma(IFN-γ) and gamma-globulin(γ-globulin) in experimental Pneumocystis carinii pneumonia of immune suppressed mice. After 9 weeks, trimethoprim-sulfamethoxazole(TMP-SMZ; 10-50 mg/mouse/day), mouse IFN-γ(5 × 104 units/mouse/day) and mouse γ-globulin(20 mg/mouse/day) were administered to the mice for 3 weeks by the experimental group. The therapeutic efficacy was evaluated by body weights, histopathologic and electron microscopic findings of the lungs, and number of P. carinii cysts by Gomori's methenamine silver stain. Body weights of the mice were significantly increased in the group of combination therapy of TMP-SMZ with IFN-γ or γ-globulin, and in the group of TMP-SMZ treatment (p < 0.05), however, little effect was found in the group of γ-globulin alone. Histopathologic findings of P. carinii pneumonia were much improved in the group of combination therapy of TMP-SMZ with IFN-γ. Treatment with either TMP-SMZ or IFN-γ significantly reduced the number of cysts in the P. carinii pneumonia, but γ-globulin alone was ineffective. In electron microscopic findings of P. carinii pneumonia, the number of trophozoites and cysts were reduced by treatment with either TMP-SMZ or IFN-γ, and most of the cysts were empty or containing one or two intracystic bodies. The present results suggested, that combination therapy of TMP-SMZ with IFN-γ had synergistic effects in treatment of P. carinii pneumonia in experimental mice.
Figures
Fig. 1 Changes of body weights of mice after anti-P. carinii therapy; each point represents the mean of 5 mice.
NOTE: I. C., infected control; Group 1, administered with trimethoprim-sulfameth-oxazole (TMP-SMZ); Group 2, administered with TMP-SMZ and mouse interferon-gamma (IFN-γ); Group 3, administered with IFN-γ; Group 4, administered with TMP-SMZ and mouse gamma-globulin (γ-globulin); Group 5, administered with γ-globulin.
Fig. 2 Changes of infectivity score of P. carinii in mice after anti-P. carinii therapy; Each bar repersents the mean±S.E. of 5 mice.
Table 1 Changes of body weight and infectivity score of P. carinii in mice injected with cortisone acetate (2.5mg twice a week)
Table 2 Changes of body weights after administration of different treatment regimens for 3 weeks on P. carinii pneumonia in mice
Table 3 Histopathologic findings of the lungs with P. carinii pneumonia in mice after anti-P. carinii therapy
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