Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81
Warning: fopen(upload/ip_log/ip_log_2024-08.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Histochemical findings of the tribocytic organ and tegument of Fibricola seoulensis
Histochemical findings of the tribocytic organ and tegument of Fibricola seoulensis
S Huh,S H Lee,* and B S Seo*
Department of Parasitology, College of Medicine, Hallym University, Chunchon 200-702, Korea.
*Department of Parasitology, Seoul National University College of Medicine, Seoul 110-460, Korea.
Abstract
The tribocytic organ and tegument of Fibricola seoulensis were examined histochemically for the detection of carbohydrates, mucosubstances, amyloid, collagen and alkaline phosphatase. The surface, secretes, gland cells of the tribocytic organ, and the tegument of the worms were positive to periodic acid Schiff (PAS) and PAS with diastase stain but negative to other stains. It was inferred that the tribocytic organ and tegument of F. seoulensis comprise neutral mucopolysaccharides, which may take a protective role against host enzymes. The surface and secretes of the tribocytic organ, and the tegument of the worms were also positive to double bridge PAP for alkaline phosphatase. This fact suggests that they may play a role as both self protective and host tissue lytic functions.
Figures
Figs. 1-9 Fig. 1. PAS reaction. Positive-red, G: gland cells of the tribocytic organ, S: secretes of the gland, TE: tegument of the worm, ×140.
Fig. 2. PAS reaction with diastase control. Positive-red, G:gland cells, S: secretes of the gland, ×140.
Table 2 Staining reactions of carbohydrates and mucosubstances
Table 3 Staining reactions of amyloid and collagen
References
1.
Chai JY. Seoul J Med 1979;20(2):104–116.
2.
Dalton JP, Heffernan M. Thiol proteases released in vitro by Fasciola hepatica. Mol Biochem Parasitol 1989;35(2):161–166.
3.
Dusanic DG. Histochemical observations of alkaline phosphatase in Schistosoma mansoni. J Infect Dis 1959;105(1):1–8.
4.
Erasmus DA, Ohman C. Electron microscope studies of the gland cells and host-parasite interface of the adhesive organ of Cyathocotyle bushiensis Khan, 1962. J Parasitol 1965;51(5):761–769.
5.
Erasmus DA, Ohman C. The Structure and Function of the Adhesive Organ in Strigeid Trematodes. Ann N Y Acad Sci 1963;113:7–35.
6.
Fujino T, Fukuda K, Hamajima F, Ishii Y. Studies on host specificity in Paragonimus westermani: II. Histochemical and cytochemical characterization of metacercariae and worms from rats and dogs. J Helminthol 1989;63(4):315–327.
7.
Huh S, Chai JY, Hong ST, Lee SH. Clinical and histopathological findings in mice heavily infected with Fibricola seoulensis. Korean J Parasitol 1988;26(1):45–53.
8.
Kaplan MM. Alkaline phosphatase. Gastroenterology 1972;62(3):452–468.
9.
Lee SH, et al. Seoul J Med 1985;23(1):52–63.
10.
Lee SH, Noh TY, Sohn WM, Kho WG, Hong ST, Chai JY. [Chronological observation of intestinal lesions of rats experimentally infected with Echinostoma hortense]. Korean J Parasitol 1990;28(1):45–52.
11.
Lee SH, Yoo BH, Hong ST, Chai JY, Seo BS, Chi JG. A histopathological study on the intestine of mice and rats experimentally infected by Fibricola Seoulensis. Korean J Parasitol 1985;23(1):58–72.
12.
Ohman C. Parasitology 1965;55:481–502.
13.
Ohman C. The structure and function of the adhesive organ in strigeid trematodes. Parasitology 1966;56(3):481–491.
14.
Sharma PN, Hanna RE. Ultrastructure and cytochemistry of the tegument of Orthocoelium scoliocoelium and Paramphistomum cervi (Trematoda: Digenea). J Helminthol 1988;62(4):331–343.