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Original Article

Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China

Woon-Mok Sohn1, Keeseon S. Eom2, Duk-Young Min3, Han-Jong Rim4, Eui-Hyug Hoang5, Yichao Yang6, Xueming Li6
The Korean Journal of Parasitology 2009;47(3):249-257.
Published online: August 28, 2009

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Corresponding author (wmsohn@gnu.ac.kr)
• Received: April 6, 2009   • Revised: May 16, 2009   • Accepted: June 5, 2009

Copyright © 2009 by The Korean Society for Parasitology

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Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Korean J Parasitol. 2009;47(3):249-257.   Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.249
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Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Korean J Parasitol. 2009;47(3):249-257.   Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.249
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Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Image Image Image Image Image
Fig. 1 (A) Haplorchis taichui metacercaria detected in a freshwater fish, Hemiculter leucisculus, from a market of Nanning City. It is elliptical, has an oral sucker (OS), a baseball glove-shaped ventrogenital sac (arrow mark) with 11-18 rodlets and an O-shaped excretory bladder (EB). Scale bar = 50 µm. (B) Haplorchis pumilio metacercaria detected in a freshwater fish, Opsariichthys bidens, from Mashan-xian. It is elliptical, has an OS, a ventrogenital complex with 36-42 deer horn-like minute spines, and an O-shaped EB occupying large portion of posterior body. Scale bar = 50 µm.
Fig. 2 (A) Centrocestus formosanus metacercaria detected in a freshwater fish, Microphysogobio fukiensis, from Mashan xian. It has 2 suckers, 32 circumoral spines around oral sucker (OS) arranged in 2 rows, and a X-shaped excretory bladder (EB) occupying greater portion of posterior body. Scale bar = 50 µm. (B) Metagonimus yokogawai metacercaria detected in a freshwater fish, Zacco platypus, from Yangshuo county. It is elliptical, has yellow brownish pigment granules scattering in body area of intestinal bifurcation, a ventral sucker (VS) deflectively located from median, and a V-shaped EB. Scale bar = 50 µm.
Fig. 3 (A) Clonorchis sinensis metacercaria detected in a freshwater fish, Hemibarbus maculates, from Yangshuo county. It is elliptical, has nearly equal sized two suckers, brownish pigment granules scattering in the body, and an O-shaped excretory bladder occupying greater part of posterior body. Scale bar = 50 µm. (B) Echinochasmus perfoliatus metacercaria detected in a freshwater fish, Zacco platypus, from Yangshuo county. It is elliptical, has a transparent and double layered cyst wall, 24 dorsally interrupted collar spines (arrow marks), and a ventral sucker (VS) lying median on posterior 1/3 of body and as large as oral sucker (OS). Scale bar = 50 µm.
Fig. 4 Metorchis taiwanensis metacercaria detected in a freshwater fish, Ctenopharyngodon idellus, from Binyang-xian. It is globular, has a double layered cyst wall (transparent and very thick outer layer and inner layer), nearly equal sized 2 suckers, and an O-shaped excretory bladder (EB). Scale bar = 50 µm.
Fig. 5 Adults of Metagonimus yokogawai (A: unstained; B: Semichon's acetocarmine stained) were recovered from an experimental dog infected with metacercariae in fish from Yangshou county. They had a rightly deviated ventral sucker (VC), which is larger than the oral sucker (OS), muscular pharynx, moderately long esophagus and ceca, transversely long seminal vesicle (SV), round or elliptical ovary (O) and seminal receptacle (SR), and 2 obliquely adjacent testes (T). The uterine loop is passing through the anterior border of both testes. Scale bar = 200 µm.

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Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Species of fish No. of fish examined in
Fusui Mashan Nanning Yangsuo Binyang Total Hemibarbus maculatus - 4 11 10 - 25 Acheilognathus tonkinensis - 2 10 6 7 25 Zacoo platypus - - - 22 - 22 Carassius auratus - 2 11 4 4 21 Hypophthalmichthys molitrix 4 1 13 - - 18 Acrossocheilus hemispinus - - - 15 - 15 Discogobio tetrabarbatus - - - 14 - 14 Hemiculter leucisculus - - 3 1 10 14 Ctenopharyngodon idella 2 - - - 10 12 Pseudohemiculter dispar - 10 - 1 - 11 Opsariichythys bidens - 8 - 3 - 11 Puntius semifasciolatus - 4 - 7 - 11 Chanodichthys erythropterus 10 - - - - 10 Microphysogobio fukiensis - 10 - - - 10 Toxabramis houdemeri - - 10 - - 10 Abbottina rivularis - - 4 5 - 9 Cyprinus carpio 1 - 4 2 - 7 Squalidus argentatus - - 7 - - 7 Metzia lineata 2 2 - - 3 7 Cirrhinus molitorella 7 - - - - 7 Sinibrama macrops - - - 6 - 6 Pseudogobio esocinus - - - 5 - 5 Aristichthys nobilis 3 - - - - 3 Culter recurviceps 1 - 2 - - 3 Squalidus wolterstorffi - - - 2 - 2 Pseudorasbora parva 1 1 - - - 2 Saurogobio dabryi - - 2 - - 2 Chanodichthys dabryi - - 1 - 1 2 Ancherythroculter lini - - - 1 - 1 Oreochromis niloticus niloticus 7 1 3 - - 11 Coreoperca whiteheadi - - - 4 - 4 Total 38 45 81 108 35 307 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Pseudohemiculter dispar 10 10 (100) 250 1-200 25.0 Opsariichthys bidens 8 8 (100) 224 4-82 28.0 Puntius semifasciolatus 4 - 9 - - Carassius auratus 2 2 (100) 39 2-37 19.5 Metzia lineata 2 1 (50.0) 5 - 5.0 Hypophthalmichthys molitrix 1 1 (100) 13 - 13.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Microphysogobio fukiensis 10 6 (60.0) 58 1-22 9.7 Pseudohemiculter dispar 10 10 (100) 1,960 30-818 196.0 Opsariichthys bidens 8 8 (100) 3,328 150-1,096 416.0 Hemibarbus maculatus 4 4 (100) 151 25-45 37.8 Puntius semifasciolatus 4 - 886 - - Carassius auratus 2 2 (100) 368 20-348 184.0 Metzia lineata 2 2 (100) 217 40-177 108.5 Hypophthalmichthys molitrix 1 1 (100) 85 - 85.0 Pseudorasbora parva 1 1 (100) 4 - 4.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Microphysogobio fukiensis 10 10 (100) 3,980 64-1,375 398.0 Metzia lineata 2 1 (50) 21 - 21.0 Pseudorasbora parva 1 1 (100) 1 - 1.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Hypophthalmichthys molitrix 13 13 (100) 691 7-159 53.2 Carassius auratus 11 11 (100) 306 4-120 27.8 Hemibarbus maculatus 11 10 (90.9) 401 6-92 40.1 Toxabramis houdemeri 10 10 (100) 1,930 1-485 193.0 Squalidus argentatus 7 3 (42.9) 45 5-20 15.0 Cyprinus carpio 4 3 (75.0) 589 1-356 196.3 Abbottina rivularis 4 2 (50.0) 14 2-12 7.0 Hemiculter leucisculus 3 3 (100) 716 105-396 238.7 Culter recurviceps 2 2 (100) 98 46-52 49.0 Saurogobio dabryi 2 2 (100) 130 35-95 65.0 Chanodichthys dabryi 1 1 (100) 55 - 55.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Hypophthalmichthys molitrix 13 12 (92.3) 748 14-153 62.3 Carassius auratus 11 11 (100) 719 16-253 65.4 Hemibarbus maculatus 11 11 (100) 289 11-52 26.3 Acheilognathus tonkinensis 10 9 (90.0) 597 27-172 66.3 Toxabramis houdemeri 10 7 (70.0) 131 5-32 18.7 Squalidus argentatus 7 7 (100) 272 3-78 38.9 Cyprinus carpio 4 2 (75.0) 380 68-312 190.0 Hemiculter leucisculus 3 3 (100) 183 14-124 61.0 Saurogobio dabryi 2 2 (100) 188 88-100 94.0 Culter recurviceps 2 2 (100) 35 15-20 17.5 Chanodichthys dabryi 1 1 (100) 12 - 12.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Acheilognathus tonkinensis 10 5 (50.0) 29 1-21 5.8 Squalidus argentatus 7 5 (71.4) 62 2-32 12.4 Cyprinus carpio 4 3 (75.0) 15 1-12 5.0 Hypophthalmichthys molitrix 4 3 (75.0) 17 2-13 5.7 Abbottina rivularis 4 4 (100) 56 7-22 14.0 Hemiculter leucisculus 3 1 (33.3) 55 - 55.0 Saurogobio dabryi 1 1 (100) 2 - 2.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Zacco platypus 22 22 (100) 9,880 23-1,836 449.1 Acrossocheilus hemispinus 15 11 (73.3) 77 2-36 7.0 Discogobio tetrabarbatus 14 12 (85.7) 64 1-18 5.3 Hemibarbus maculatus 10 10 (100) 2,484 14-731 248.4 Puntius semifasciolatus 7 5 (71.4) 967 87-293 193.4 Sinibrama macrops 6 6 (100) 123 10-30 20.5 Acheilognathus tonkinensis 6 2 (33.3) 5 1-4 2.5 Abbottina rivularis 5 5 (100) 559 14-311 111.8 Pseudogobio esocinus 5 1 (20.0) 32 - 32.0 Carassius auratus 4 1 (25.0) 1 - 1.0 Coreoperca whiteheadi 4 1 (25.0) 2 - 2.0 Hemiculter sp. 4 3 (75.0) 224 5-209 74.7 Opsariichthys bidens 3 3 (100) 114 30-45 38.0 Squalidus wolterstorffi 2 2 (100) 31 3-28 15.5 Cyprinus carpio 2 1 (50.0) 13 - 13.0 Ancherythroculter lini 1 1 (100) 26 - 26.0 Pseudohemiculter dispar 1 1 (100) 31 - 31.0 Hemiculter leucisculus 1 1 (100) 34 - 34.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Hemiculter leucisculus 10 10 (100) 206 5-44 20.6 Ctenopharyngodon idella 10 10 (100) 712 22-144 71.2 Acheilognathus tonkinensis 7 7 (100) 732 68-150 104.6 Carassius auratus 4 4 (100) 334 42-125 83.5 Metzia lineata 3 3 (100) 62 17-23 20.7 Species of flukes No. of fish host Fish hosts infected with more than 100 metacercariae/fish Metagonimus yokogawai 18 Z. platypus, A. rivularis, H. maculatus, P. Semifasiolatus Haplorchis taichui 15 H. leucisculus, C. carpio, T. houdemeri Haplorchis pumilio 18 C. auratus, C. carpio, A. tonkinensis, O. bidens, M. lineata, P. dispar, P. Semifasiolatus Centrocestus formosanus 10 M. fukiensis
Table 1. Freshwater fishes collected from 5 localities in Guangxi Zhuang Autonomous Region, China
Table 2. Infection status of Haplorchis taichui metacercariae by the species of fish from a stream in Mashan-xian
Table 3. Infection status of Haplorchis pumilio metacercariae by the species of fish from a stream in Mashan-xian
Table 4. Infection status of Centrocestus formosanus metacercariae by the species of fish from a stream in Mashan-xian
Table 5. Infection status of Haplorchis taichui metacercariae by the species of fish from a market in Nanning City
Table 6. Infection status of Haplorchis pumilio metacercariae by the species of fish from a market in Nanning City
Table 7. Infection status of Centrocestus formosanus metacercariae by the species of fish from a market in Nanning City
Table 8. Infection status of Metagonimus yokogawai metacercariae by the species of fish collected from Yangshuo County
Table 9. Infection status of Haplorchis pumilio metacercariae by the species of fish from Binyang-xian
Table 10. Fish hosts infected with more than 100 metacercariae per fish by the trematode species

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PHD : Parasites, Hosts and Diseases

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