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Woo-Yoon Park	9 Articles
Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea Seobo Sim, Jua Won, Jae-Whan Kim, Kyungjin Kim, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2017;55(2):137-142. Published online April 30, 2017 DOI: https://doi.org/10.3347/kjp.2017.55.2.137 Abstract PDF Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragmentation and nested PCR confirmed Cryptosporidium parvum and Cyclospora cayetanensis, respectively. In conclusion, we detected C. cayetanensis in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea. Citations Citations to this article as recorded by Parasitic Contamination of Fresh Vegetables Used for Salads in Corum, Türkiye: Molecular and Microscopic Evidence Sabiha Sensoz, Djursun Karasartova, Gonul Arslan Akveran, Nezahat Askin, Meral Aydenizoz, Ayse Semra Gureser, Hamid Soori, Aysegul Taylan-Ozkan Foodborne Pathogens and Disease.2026;[Epub] CrossRef Intestinal zoonotic parasites in companion animals and potential of human exposure in the Middle East Haifaa A. Mahjoub Veterinary Parasitology: Regional Studies and Reports.2026; 67: 101413. CrossRef Cryptosporidium and cryptosporidiosis: An update of Asian perspectives in humans, water and food, 2015–2025 Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Mohammad Reza Mahmoudi, Huma Khan, Simuzar Mamedova, Ardra Namboodiri, Frederick R. Masangkay, Panagiotis Karanis Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100311. CrossRef Cyclospora in humans, animals, fresh produce and water in China: implications for host specificity of Cyclospora species and zoonotic transmission of C. cayetanensis Kangli Feng, Yaqiong Guo, Na Li, Lihua Xiao, Yaoyu Feng One Health Advances.2025;[Epub] CrossRef Cryptosporidium and agriculture: A review Eleni Golomazou, Simuzer Mamedova, Aida Vafae Eslahi, Panagiotis Karanis Science of The Total Environment.2024; 916: 170057. CrossRef Unveiling risks in healthy food: Vegetables and fruits are linked to the distribution chain of protozoan parasites Aida Vafae Eslahi, Simuzer Mamedova, Reghaissia Nassiba, Panagiotis Karanis Food Microbiology.2024; 123: 104592. CrossRef Food and Waterborne Cryptosporidiosis from a One Health Perspective: A Comprehensive Review Munwar Ali, Yaru Ji, Chang Xu, Qazal Hina, Usama Javed, Kun Li Animals.2024; 14(22): 3287. CrossRef Loop mediated isothermal amplification for detection of foodborne parasites: A journey from lab to lab-on-a-chip Fatemeh Mahdavi Abhari, Maryam Niyyati, Hamid Assadzadeh Aghdaei, Hamed Mirjalali Food Control.2023; 143: 109251. CrossRef Health risks of Cryptosporidium and Giardia in the application of surface water and septic tank effluent in Chinese agriculture: Impact on cancer patients identified by quantitative microbial risk assessment Qian Huang, Shan Huang, Weijie Kuang, Jianghui Yi, Shunxin Xiao, Feng Zhao, Guosheng Xiao Food Microbiology.2023; 111: 104213. CrossRef Development of a new multiplex PCR to detect fecal coccidian parasite Manish Katiyar, Reena Gulati, Nonika Rajkumari, Rakesh Singh Indian Journal of Gastroenterology.2023; 42(2): 241. CrossRef Contamination of fresh produce sold on the Italian market with Cyclospora cayetanensis and Echinococcus multilocularis Alessandra Barlaam, Tamirat T. Temesgen, Kristoffer R. Tysnes, Laura Rinaldi, Nicola Ferrari, Anna R. Sannella, Giovanni Normanno, Simone M. Cacciò, Lucy J. Robertson, Annunziata Giangaspero Food Microbiology.2021; 98: 103792. CrossRef Detection of Cyclospora cayetanensis on bagged pre-cut salad mixes within their shelf-life and after sell by date by the U.S. food and drug administration validated method Sonia Almeria, Alicia Shipley Food Microbiology.2021; 98: 103802. CrossRef Diverse Genotypes and Species of Cryptosporidium in Wild Rodent Species from the West Coast of the USA and Implications for Raw Produce Safety and Microbial Water Quality Xunde Li, Edward Robert Atwill Microorganisms.2021; 9(4): 867. CrossRef Detection of Cyclospora cayetanensis, Echinococcus multilocularis, Toxocara spp. and microsporidia in fresh produce using molecular methods: – A review B. Bartosova, B. Koudela, I. Slana Food and Waterborne Parasitology.2021; 23: e00124. CrossRef Causes of acute gastroenteritis in Korean children between 2004 and 2019 Eell Ryoo Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef An Epidemiological and Diagnostic Study of Cyclospora Cayetanensis Parasite in Anbar Province - Iraq S S Shahatha, S A Alkubaisy, M O Mousa IOP Conference Series: Earth and Environmental Science.2021; 904(1): 012026. CrossRef Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries Angela Assurian, Helen Murphy, Laura Ewing, Hediye Nese Cinar, Alexandre da Silva, Sonia Almeria Food Microbiology.2020; 87: 103397. CrossRef A Molecular Tool for Rapid Detection and Traceability of Cyclospora cayetanensis in Fresh Berries and Berry Farm Soils Carolina N. Resendiz-Nava, Guadalupe E. Orozco-Mosqueda, Edmundo M. Mercado-Silva, Susana Flores-Robles, Hilda V. Silva-Rojas, Gerardo M. Nava Foods.2020; 9(3): 261. CrossRef Parasite detection in food: Current status and future needs for validation Rachel M. Chalmers, Lucy J. Robertson, Pierre Dorny, Suzanne Jordan, Age Kärssin, Frank Katzer, Stéphanie La Carbona, Marco Lalle, Brian Lassen, Ivona Mladineo, Miroslaw Rozycki, Ewa Bilska-Zajac, Gereon Schares, Anne Mayer-Scholl, Chiara Trevisan, Kristo Trends in Food Science & Technology.2020; 99: 337. CrossRef Detection of Cryptosporidium oocysts and Giardia cysts in vegetables from street markets from the Qinghai Tibetan Plateau Area in China Xiuping Li, Xueyong Zhang, Yingna Jian, Geping Wang, Liqing Ma, Chad Schou, Panagiotis Karanis Parasitology Research.2020; 119(6): 1847. CrossRef Comparison of commercial and in-house real-time PCR platforms for 15 parasites and microsporidia in human stool samples without a gold standard Thomas Köller, Andreas Hahn, Enkhtsetseg Altangerel, Jaco J. Verweij, Olfert Landt, Simone Kann, Denise Dekker, Jürgen May, Ulrike Loderstädt, Andreas Podbielski, Hagen Frickmann Acta Tropica.2020; 207: 105516. CrossRef Prevalence of Cryptosporidium and Giardia in vegetables in Iran: a nineteen-years meta-analysis review Ehsan Javanmard, Elnaz Sadat Mirsamadi, Meysam Olfatifar, Erfan Ghasemi, Fatemeh Saki, Hamed Mirjalali, Mohammad Reza Zali, Panagiotis Karanis Journal of Environmental Health Science and Engineering.2020; 18(2): 1629. CrossRef Detection of human intestinal protozoan parasites in vegetables and fruits: a review Junqiang Li, Zhenzhen Wang, Md Robiul Karim, Longxian Zhang Parasites & Vectors.2020;[Epub] CrossRef Human cyclosporiasis Annunziata Giangaspero, Robin B Gasser The Lancet Infectious Diseases.2019; 19(7): e226. CrossRef Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay Karen Shapiro, Minji Kim, Veronica B. Rajal, Michael J. Arrowood, Andrea Packham, Beatriz Aguilar, Stefan Wuertz Food Microbiology.2019; 84: 103252. CrossRef Status of common parasitic diseases in Korea in 2019 Sun Huh Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef Genotyping genetically heterogeneousCyclospora cayetanensisinfections to complement epidemiological case linkage Joel L. N. Barratt, Subin Park, Fernanda S. Nascimento, Jessica Hofstetter, Mateusz Plucinski, Shannon Casillas, Richard S. Bradbury, Michael J. Arrowood, Yvonne Qvarnstrom, Eldin Talundzic Parasitology.2019; 146(10): 1275. CrossRef Identification of human pathogenic Enterocytozoon bieneusi, Cyclospora cayetanensis, and Cryptosporidium parvum on the surfaces of vegetables and fruits in Henan, China Junqiang Li, Ke Shi, Fangfang Sun, Tingwen Li, Rongjun Wang, Sumei Zhang, Fuchun Jian, Changshen Ning, Longxian Zhang International Journal of Food Microbiology.2019; 307: 108292. CrossRef Cyclospora cayetanensis and Cyclosporiasis: An Update Sonia Almeria, Hediye N. Cinar, Jitender P. Dubey Microorganisms.2019; 7(9): 317. CrossRef Cryptosporidium spp., prevalence, molecular characterisation and socio-demographic risk factors among immigrants in Qatar Sonia Boughattas, Jerzy M. Behnke, Duaa Al-Sadeq, Ahmed Ismail, Marawan Abu-Madi, Christine A. Petersen PLOS Neglected Tropical Diseases.2019; 13(10): e0007750. CrossRef Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016 Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee The Korean Journal of Parasitology.2019; 57(5): 531. CrossRef Assessment of pesticide residues and microbial contamination in raw leafy green vegetables marketed in Italy Gino Angelo Santarelli, Giacomo Migliorati, Francesco Pomilio, Cristina Marfoglia, Patrizia Centorame, Antonella D'Agostino, Roberta D'Aurelio, Rossana Scarpone, Noemi Battistelli, Federica Di Simone, Giuseppe Aprea, Luigi Iannetti Food Control.2018; 85: 350. CrossRef Parasite contamination of berries: Risk, occurrence, and approaches for mitigation Tamirat Tefera, Kristoffer R. Tysnes, Kjersti Selstad Utaaker, Lucy J. Robertson Food and Waterborne Parasitology.2018; 10: 23. CrossRef 12,325 View 284 Download 35 Web of Science Crossref Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP Hyeng-Il Cheun, Kyungjin Kim, Sejoung Yoon, Won-Ja Lee, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu Korean J Parasitol 2013;51(3):353-355. Published online June 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.3.353 Abstract PDF There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections. Citations Citations to this article as recorded by Causes of acute gastroenteritis in Korean children between 2004 and 2019 Eell Ryoo Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef Cryptosporidium spp. Diagnosis and Research in the 21st Century Jennifer K. O'Leary, Roy D. Sleator, Brigid Lucey Food and Waterborne Parasitology.2021; 24: e00131. CrossRef Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples Felix Weinreich, Andreas Hahn, Kirsten Alexandra Eberhardt, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt Pathogens.2021; 10(9): 1131. CrossRef Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofCryptosporidiumSpecies in Kenyan Children Presenting with Diarrhea Timothy S. Mamba, Cecilia K. Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K. Njiru Journal of Tropical Medicine.2018; 2018: 1. CrossRef Expression and Purification of gp40/15 Antigen of Cryptosporidium parvum Parasite in Escherichia coli: an Innovative Approach in Vaccine Production Hossein Sobati, Habib Jasor-Gharebagh, Hossein Honari Iranian Red Crescent Medical Journal.2017;[Epub] CrossRef Detection of Cryptosporidium parvum in Environmental Soil and Vegetables Semie Hong, Kyungjin Kim, Sejoung Yoon, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu Journal of Korean Medical Science.2014; 29(10): 1367. CrossRef 10,902 View 150 Download Crossref Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2011;49(1):25-31. Published online March 18, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.1.25 Abstract PDF Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented. Citations Citations to this article as recorded by Real-time detection of 3–8-µm microbial particles in sand filter effluent as a surrogate indicator of Cryptosporidium parvum oocysts Sandrine Boivin, Hiroshi Nagaoka, Takahiro Fujioka Water Research X.2025; 27: 100345. CrossRef Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert Parasitology Research.2023; 122(8): 1819. CrossRef Discovery of New Microneme Proteins in Cryptosporidium parvum and Implication of the Roles of a Rhomboid Membrane Protein (CpROM1) in Host–Parasite Interaction Xin Gao, Jigang Yin, Dongqiang Wang, Xiaohui Li, Ying Zhang, Chenchen Wang, Yuanyuan Zhang, Guan Zhu Frontiers in Veterinary Science.2021;[Epub] CrossRef A new method for efficient detection of Cryptosporidium RNA by real-time reverse transcription-PCR with surfactants Takahiro Sekikawa, Kosuke Toshiki Water Supply.2015; 15(5): 1061. CrossRef 8,858 View 92 Download Crossref Multiplex PCR Detection of Waterborne Intestinal Protozoa: Microsporidia, Cyclospora, and Cryptosporidium Seung-Hyun Lee, Migyo Joung, Sejoung Yoon, Kyoungjin Choi, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2010;48(4):297-301. Published online December 16, 2010 DOI: https://doi.org/10.3347/kjp.2010.48.4.297 Abstract PDF Recently, emerging waterborne protozoa, such as microsporidia, Cyclospora, and Cryptosporidium, have become a challenge to human health worldwide. Rapid, simple, and economical detection methods for these major waterborne protozoa in environmental and clinical samples are necessary to control infection and improve public health. In the present study, we developed a multiplex PCR test that is able to detect all these 3 major waterborne protozoa at the same time. Detection limits of the multiplex PCR method ranged from 10¹ to 10² oocysts or spores. The primers for microsporidia or Cryptosporidium used in this study can detect both Enterocytozoon bieneusi and Encephalitozoon intestinalis, or both Cryptosporidium hominis and Cryptosporidium parvum, respectively. Restriction enzyme digestion of PCR products with BsaBI or BsiEI makes it possible to distinguish the 2 species of microsporidia or Cryptosporidium, respectively. This simple, rapid, and cost-effective multiplex PCR method will be useful for detecting outbreaks or sporadic cases of waterborne protozoa infections. Citations Citations to this article as recorded by Development of a new multiplex PCR to detect fecal coccidian parasite Manish Katiyar, Reena Gulati, Nonika Rajkumari, Rakesh Singh Indian Journal of Gastroenterology.2023; 42(2): 241. CrossRef Use of DNA nanosensors based on upconverting nanoparticles for detection of Nosema bombycis by fluorescence resonance energy transfer Ping He, Hongli Chen, Jiancheng Sun, Qiang Wang, Xudong Tang, Yiling Zhang, Feng Zhu, Zhongyuan Shen Folia Microbiologica.2022; 67(3): 419. CrossRef Human Microsporidiosis: A Narrative Review of Serodiagnostic Tests Samaneh Abdolahi Khabisi International Journal of Infection.2022;[Epub] CrossRef First Identification and Genotyping of Enterocytozoon bieneusi and Prevalence of Encephalitozoon intestinalis in Patients with Acute Diarrhea in the Republic of Korea Ji-Young Kwon, Ji-Ye Seo, Tae-Yun Kim, Hee-Il Lee, Jung-Won Ju Pathogens.2021; 10(11): 1424. CrossRef Rapid diagnosis of parasitic diseases: current scenario and future needs S. Momčilović, C. Cantacessi, V. Arsić-Arsenijević, D. Otranto, S. Tasić-Otašević Clinical Microbiology and Infection.2019; 25(3): 290. CrossRef Assessment of the first commercial multiplex PCR kit (ParaGENIE Crypto-Micro Real-Time PCR) for the detection of Cryptosporidium spp., Enterocytozoon bieneusi, and Encephalitozoon intestinalis from fecal samples Florent Morio, Philippe Poirier, Yohann Le Govic, Adrien Laude, Stéphane Valot, Guillaume Desoubeaux, Nicolas Argy, Céline Nourrisson, Christelle Pomares, Marie Machouart, Frédéric Dalle, Françoise Botterel, Nathalie Bourgeois, Estelle Cateau, Marion Lete Diagnostic Microbiology and Infectious Disease.2019; 95(1): 34. CrossRef Microfluidic multi-angle laser scattering system for rapid and label-free detection of waterborne parasites Wei Huang, Limei Yang, Gang Yang, Feng Li Biomedical Optics Express.2018; 9(4): 1520. CrossRef Opportunistic and non-opportunistic intestinal parasites in HIV/ AIDS patients in relation to their clinical and epidemiological status in a specialized medical service in Goiás, Brazil Natane Barbosa Barcelos, Lorena de Freitas e Silva, Regyane Ferreira Guimarães Dias, Hélio Ranes de Menezes Filho, Rosângela Maria Rodrigues Revista do Instituto de Medicina Tropical de São Paulo.2018;[Epub] CrossRef Detection Methodologies for Pathogen and Toxins: A Review Md Alahi, Subhas Mukhopadhyay Sensors.2017; 17(8): 1885. CrossRef Detection and Molecular Characterization of <i>Cryptosporidium</i> spp. from Wild Rodents and Insectivores in South Korea Juha Song, C-Yoon Kim, Seo-Na Chang, Tamer Said Abdelkader, Juhee Han, Tae-Hyun Kim, Hanseul Oh, Ji Min Lee, Dong-Su Kim, Jong-Taek Kim, Hong-Shik Oh, Moonsuk Hur, Jae-Hwa Suh, Jae-Hak Park The Korean Journal of Parasitology.2015; 53(6): 737. CrossRef Detection of Nosema bombycis by FTA Cards and Loop-Mediated Isothermal Amplification (LAMP) Wei Yan, Zhongyuan Shen, Xudong Tang, Li Xu, Qianlong Li, Yajie Yue, Shengyan Xiao, Xuliang Fu Current Microbiology.2014; 69(4): 532. CrossRef Molecular Analysis of the Alpha-Tubulin Gene from the Microsporidium, Endoreticulatus sp. Zhenjiang, Isolated from Bombyx mori Shengyan Xiao, Zhongyuan Shen, Xudong Tang, Li Xu, Xuliang Fu, Yajie Yue, Nan Li, Wei Wang Agricultural Sciences.2014; 05(10): 882. CrossRef Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections Jaco J. Verweij, C. Rune Stensvold Clinical Microbiology Reviews.2014; 27(2): 371. CrossRef Intestinal protozoan infections in the immunocompromised host Luis A. Marcos, Eduardo Gotuzzo Current Opinion in Infectious Diseases.2013; 26(4): 295. CrossRef Evaluation of formalin-ether sedimentation and trichrome staining techniques: Its effectiveness in detecting Entamoeba histolytica/dispar/moshkovskii in stool samples Tengku Shahrul Anuar, Hesham M. Al-Mekhlafi, Mohamed Kamel Abdul Ghani, Edariah Abu Bakar, Siti Nor Azreen, Fatmah Md Salleh, Norhayati Moktar Journal of Microbiological Methods.2013; 92(3): 344. CrossRef Epidemiological Characteristics of the First Water-Borne Outbreak of Cryptosporidiosis in Seoul, Korea Shinje Moon, Wooseok Kwak, Sangwon Lee, Won Kim, Jaeyeon Oh, Seung-Ki Youn Journal of Korean Medical Science.2013; 28(7): 983. CrossRef Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin Hend Aly El-Taweel, Mona Mohammed Tolba, Hayam Abdelmonem Sadaka, Lobna Abdelaziz El-Zawawy, Mervat Mostafa Osman Parasitology Research.2012; 111(2): 689. CrossRef Microsporidiosis Elizabeth S. Didier, Louis M. Weiss Current Opinion in Infectious Diseases.2011; 24(5): 490. CrossRef 11,403 View 121 Download Crossref A Hospital-Based Serological Survey of Cryptosporidiosis in the Republic of Korea Jong-Kyu Lee, Eun-Taek Han, Sun Huh, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2009;47(3):219-225. Published online August 28, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.3.219 Abstract PDF The seroprevalence of cryptosporidiosis was examined using patients' sera collected from hospitals located in 4 different areas of the Republic of Korea. ELISA was used to measure antibody titers against Cryptosporidium parvum antigens from a total of 2,394 serum samples, which were collected randomly from patients in local hospitals; 1) Chungbuk National University Hospital, 2) Konkuk University Hospital, 3) local hospitals in Chuncheon, Gangwon-do (province), 4) Jeonnam National University Hospital, from 2002 through 2003. Of the 2,394 samples assayed, 34%, 26%, and 56% were positive for C. parvum-specific IgG, IgM, and IgA antibodies, respectively. Positive IgG titers were most common in sera from Jeonnam National University Hospital, Gwangju, Jeollanam-do, and positive IgM titers were most common in sera from Chungbuk National University Hospital, Cheongju, Chuncheongbuk-do. The seropositivity was positively correlated with age for both the IgG and IgA antibodies but was negatively correlated with age for the IgM antibodies. Western blotting revealed that 92%, 83%, and 77% of sera positive for IgG, IgM, and IgA ELISA reacted with 27-kDa antigens, respectively. These results suggested that infection with Cryptosporidium in hospital patients occurs more commonly than previously reported in the Republic of Korea. Citations Citations to this article as recorded by A Waterborne Outbreak and Detection of Cryptosporidium Oocysts in Drinking Water of an Older High-Rise Apartment Complex in Seoul Eun-Joo Cho, Jin-Young Yang, Eun-Sook Lee, Se-Chul Kim, So-Yang Cha, Sung-Tek Kim, Man-Ho Lee, Sun-Hee Han, Young-Sang Park The Korean Journal of Parasitology.2013; 51(4): 461. CrossRef Clinical Guidelines for the Treatment and Prevention of Opportunistic Infections in HIV-infected Koreans Infection & Chemotherapy.2012; 44(3): 93. CrossRef Serum IgG Responses and Seroconversion Patterns to Cryptosporidium gp15 among Children in a Birth Cohort in South India Rajiv Sarkar, Sitara Swarna Rao Ajjampur, Jayaprakash Muliyil, Honorine Ward, Elena N. Naumova, Gagandeep Kang Clinical and Vaccine Immunology.2012; 19(6): 849. CrossRef Characterization of the thioredoxin peroxidase from Cryptosporidium parvum Migyo Joung, Sejoung Yoon, Kyungmi Choi, Joung-Yeon Kim, Woo-Yoon Park, Jae-Ran Yu Experimental Parasitology.2011; 129(4): 331. CrossRef Cryptosporidiosis Jae-Ran Yu Hanyang Medical Reviews.2010; 30(3): 187. CrossRef 9,866 View 70 Download Crossref Comparative Sensitivity of PCR Primer Sets for Detection of Cryptosporidium parvum Jae-Ran Yu, Soo-Ung Lee, Woo-Yoon Park Korean J Parasitol 2009;47(3):293-297. Published online August 28, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.3.293 Abstract PDF Improved methods for detection of Cryptosporidium oocysts in environmental and clinical samples are urgently needed to improve detection of cryptosporidiosis. We compared the sensitivity of 7 PCR primer sets for detection of Cryptosporidium parvum. Each target gene was amplified by PCR or nested PCR with serially diluted DNA extracted from purified C. parvum oocysts. The target genes included Cryptosporidium oocyst wall protein (COWP), small subunit ribosomal RNA (SSU rRNA), and random amplified polymorphic DNA. The detection limit of the PCR method ranged from 10³ to 10⁴ oocysts, and the nested PCR method was able to detect 10⁰ to 10² oocysts. A second-round amplification of target genes showed that the nested primer set specific for the COWP gene proved to be the most sensitive one compared to the other primer sets tested in this study and would therefore be useful for the detection of C. parvum. Citations Citations to this article as recorded by Occurrence and molecular characterization of Cryptosporidium oocysts in chickens from Egypt, and a meta-analysis for Cryptosporidium infections in chickens worldwide Ahmed Essam, Bassem Elmishmishy, Enas Hammad, Salah Abu Elwafa, Ibrahim Abbas Veterinary Parasitology: Regional Studies and Reports.2025; 57: 101169. CrossRef Evaluating the role of synanthropic filth flies in the transmission of zoonotic parasites: field and laboratory evidence from different animal rearing sites in upper Egypt with focus on Cryptosporidium spp. Omaima Ragab AbdAllah, Refaat M. Gabre, Sara Abdelaal Mohammed, Ahmed Mohamed Korayem, Hala E. Hussein, Alzahraa Abdelraouf Ahmad BMC Veterinary Research.2025;[Epub] CrossRef Chemical profiling of Verbena officinalis and assessment of its anti-cryptosporidial activity in experimentally infected immunocompromised mice Eman S. El-Wakil, Maha A.M. El-Shazly, Ayman M. El-Ashkar, Tarek Aboushousha, Mosad A. Ghareeb Arabian Journal of Chemistry.2022; 15(7): 103945. CrossRef Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice Eman S. El-Wakil, Eman Ali Mohamed, Eman Ahmed El-Wakil, Tarek S. AbouShousha, Neimat Mousa Amer Acta Protozoologica.2022; 61: 23. CrossRef Detection and Molecular Identification of Cryptosporidium Species Among Children with Malignancies Heba Said Ibrahim, Amel Youssef Shehab, Amal Farahat Allam, Mostafa Aboelhoda Mohamed, Hoda Fahmy Farag, Mona Mohamed Tolba Acta Parasitologica.2021; 66(2): 377. CrossRef Competency Assessment: Diagnostic Methods for Detection of Cryptosporidium, Microsporidia, and Toxoplasma in Bronchoalveolar Lavage Samples Zahra Eslamirad, Abdolatif Moini, Mojtaba Didehdar, Reza Hajihossein, Ali Arash Anoushiravani Jundishapur Journal of Microbiology.2021;[Epub] CrossRef Molecular epidemiology of Cryptosporidium spp. in an agricultural area of northern Vietnam: A community survey Hanako Iwashita, Taichiro Takemura, Asako Tokizawa, Tetsuhiro Sugamoto, Vu Dinh Thiem, Tuan Hai Nguyen, Tho Duc Pham, Anh Hong Quynh Pham, Hang Thi Doan, Na Ly Tran, Tetsu Yamashiro Parasitology International.2021; 83: 102341. CrossRef Genotype and subtype analyses of Cryptosporidium isolate from humans by gp60 PCR-RLFP in Zabol, Southeast of Iran Mansour Dabirzadeh, Habibeh Mohammadian, Hakim Azizi, Mahdi Khoshsima Shahreki Modern Medical Laboratory Journal.2021; 4(1): 5. CrossRef In vitro and in vivo anti-Cryptosporidium and anti-inflammatory effects of Aloe vera gel in dexamethasone immunosuppressed mice Alyaa Farid, Aya Tawfik, Basil Elsioufy, Gehan Safwat International Journal for Parasitology: Drugs and Drug Resistance.2021; 17: 156. CrossRef Prevalence and molecular characterization of Cryptosporidium species in poultry in Bangladesh Mohammad Hazzaz Bin Kabir, Yongmei Han, Seung-Hun Lee, Arifin Budiman Nugraha, Frances Recuenco, Fumi Murakoshi, Xuenan Xuan, Kentaro Kato One Health.2020; 9: 100122. CrossRef Determination of the Microbial and Chemical Loads in Rivers from the Quito Capital Province of Ecuador (Pichincha)—A Preliminary Analysis of Microbial and Chemical Quality of the Main Rivers Pamela Borja-Serrano, Valeria Ochoa-Herrera, Laurence Maurice, Gabriela Morales, Cristian Quilumbaqui, Eduardo Tejera, António Machado International Journal of Environmental Research and Public Health.2020; 17(14): 5048. CrossRef The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples Harith Saeed Al-Warid, Ihsan M. Al-Saqur, Souhaila H. Mahmood National Academy Science Letters.2019; 42(5): 423. CrossRef Molecular characterization of zoonotic Cryptosporidium spp. and Giardia duodenalis pathogens in Algerian sheep Lynda Sahraoui, Myriam Thomas, Aurélie Chevillot, Mohamed Mammeri, Bruno Polack, Isabelle Vallée, Jérôme Follet, Hacina Ain-Baaziz, Karim Tarik Adjou Veterinary Parasitology: Regional Studies and Reports.2019; 16: 100280. CrossRef Genetic Study of Cryptosporidium with SSU-rRNA in Children Younger Than Ten Referring to Hospitals of Zabol, Southeast of Iran Habibeh Mohammadian, Hekim Azizi, Mansour Dabirzadeh Shiraz E-Medical Journal.2019;[Epub] CrossRef Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France Mohamed Mammeri, Aurélie Chevillot, Ilham Chenafi, Myriam Thomas, Christine Julien, Isabelle Vallée, Bruno Polack, Jérôme Follet, Karim Tarik Adjou Veterinary Parasitology: Regional Studies and Reports.2019; 18: 100323. CrossRef Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016 Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee The Korean Journal of Parasitology.2019; 57(5): 531. CrossRef Monitoring of Noxious Protozoa for Management of Natural Water Resources Young Yil Bahk, Pyo Yun Cho, Sung Kyu Ahn, Sangjung Park, Won Hwa Jheong, Yun-Kyu Park, Ho-Joon Shin, Sang-Seob Lee, Okjae Rhee, Tong-Soo Kim The Korean Journal of Parasitology.2018; 56(2): 205. CrossRef Intestinal Cryptosporidiosis in Renal Transplant Recipients: Prevalence, Species Detection and Comparative Evaluation of SSU rRNA and Cryptosporidium Oocyst Wall Protein Genes Ujjala Ghoshal, Prabhat Ranjan, Asmita Dey, Uday Chand Ghoshal Indian Journal of Medical Microbiology.2018; 36(2): 247. CrossRef Checking the detail in retail: Occurrence of Cryptosporidium and Giardia on vegetables sold across different counters in Chandigarh, India Kjersti Selstad Utaaker, Anil Kumar, Himanshu Joshi, Suman Chaudhary, Lucy J. Robertson International Journal of Food Microbiology.2017; 263: 1. CrossRef RT-PCR specific for Cryspovirus is a highly sensitive method for detecting Cryptosporidium parvum oocysts Mark Jenkins, Celia O'Brien, Raymond Fetterer, Monica Santin Food and Waterborne Parasitology.2016; 5: 14. CrossRef Molecular detection and characterization of Cryptosporidium spp. among breeding cattery cats in Japan Yoichi Ito, Naoyuki Itoh, Yuya Kimura, Kazutaka Kanai Parasitology Research.2016; 115(5): 2121. CrossRef Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin The Korean Journal of Parasitology.2016; 54(5): 631. CrossRef Survey for protozoan parasites in Eastern oysters ( Crassostrea virginica ) from the Gulf of Maine using PCR-based assays Nicholas D. Marquis, Nicholas R. Record, José A. Fernández Robledo Parasitology International.2015; 64(5): 299. CrossRef Cryptosporidium species and subtype analysis in diarrhoeic pre-weaned lambs and goat kids from north-western Spain Pablo Díaz, Joaquín Quílez, Alberto Prieto, Esther Navarro, Ana Pérez-Creo, Gonzalo Fernández, Rosario Panadero, Ceferino López, Pablo Díez-Baños, Patrocinio Morrondo Parasitology Research.2015; 114(11): 4099. CrossRef A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India Beena Uppal, Ompal Singh, Sanjim Chadha, Arun Kumar Jha Journal of Parasitology Research.2014; 2014: 1. CrossRef Polymerase chain reaction and nested-PCR approaches for detecting Cryptosporidium in water catchments of water treatment plants in Curitiba, State of Paraná, Brazil Silvia Cristina Osaki, Vanete Thomaz Soccol, Adriana Oliveira Costa, Marcia Benedita Oliveira-Silva, Juliana Tracz Pereira, Antonio Eduardo Procopio Revista da Sociedade Brasileira de Medicina Tropical.2013; 46(3): 270. CrossRef Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples P. Goñi, B. Martín, M. Villacampa, A. García, C. Seral, F. J. Castillo, A. Clavel European Journal of Clinical Microbiology & Infectious Diseases.2012; 31(8): 2077. CrossRef Detection of Cryptosporidium parvum and Cryptosporidium hominis in human patients in Cairo, Egypt Nour M. Abd El Kader, María-Alejandra Blanco, Marwa Ali-Tammam, Abd El Rahman B. Abd El Ghaffar, Ahmed Osman, Nabila El Sheikh, José Miguel Rubio, Isabel de Fuentes Parasitology Research.2012; 110(1): 161. CrossRef Epidemiology and Molecular Relationships of Cryptosporidium spp. in People, Primates, and Livestock from Western Uganda Stephanie J. Salyer, Thomas R. Gillespie, Innocent B. Rwego, Colin A. Chapman, Tony L. Goldberg, Joseph M. Vinetz PLoS Neglected Tropical Diseases.2012; 6(4): e1597. CrossRef Cryptosporidiosis Jae-Ran Yu Hanyang Medical Reviews.2010; 30(3): 187. CrossRef 18,255 View 172 Download Crossref Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera Jong-Gyu Lee, Eun-Taek Han, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2009;47(2):171-174. Published online May 27, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.2.171 Abstract PDF The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at various developmental stages of parasites, including asexual, sexual stages, and oocysts. The amount of antigen-stimulating IgG antibodies was particularly high on the oocyst wall. The sporozoite surface was shown to give stimulation on IgG and IgM antibody formation. Trophozoites implicated the lowest antigenicity to humoral immunity, both IgG and IgM, by showing the least amount of gold labeling. Immunogold labeling also provided clues that antigens were presented to the host-cell cytoplasm via feeder organelles and host-parasite junctions. Citations Citations to this article as recorded by Cryptostatin, a chagasin-family cysteine protease inhibitor ofCryptosporidium parvum J.-M. KANG, H.-L. JU, J.-R. YU, W.-M. SOHN, B.-K. NA Parasitology.2012; 139(8): 1029. CrossRef 10,358 View 128 Download Crossref Quantitative Evaluation of Infectivity Change of Cryptosporidium parvum after Gamma Irradiation Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2009;47(1):7-11. Published online March 12, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.1.7 Abstract PDF Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C. parvum after gamma irradiation and repair of the infectivity during incubation time after irradiation. C. parvum oocysts were subjected to gamma irradiation at various doses (1, 5, 10, and 25 kGy), and the in vitro infectivity was measured by real-time PCR every day up to 7 days after irradiation. The in vitro infectivity of C. parvum on human ileocecal adenocarcinoma cells (HCT-8) was effectively reduced (> 2 log₁₀) by irradiation at 10 kGy or more. However, in the experiment to find out repair of the infectivity, recovery was not noted until day 7 post-incubation. Citations Citations to this article as recorded by Ultrastructural Changes inCryptosporidium parvumOocysts by Gamma Irradiation Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2011; 49(1): 25. CrossRef Effect of Infrared Irradiation of Oocysts on the Pathology of Experimental Cryptosporidiosis in Apparently Normal and Immunosuppressed Mice Hafidh I. Al-Sadi, Saevan S. Al-Mahm Research Journal of Biological Sciences.2010; 5(2): 177. CrossRef Cryptosporidiosis Jae-Ran Yu Hanyang Medical Reviews.2010; 30(3): 187. CrossRef Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu Experimental Parasitology.2010; 125(3): 230. CrossRef 8,449 View 67 Download Crossref In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2004;42(1):27-34. Published online March 20, 2004 DOI: https://doi.org/10.3347/kjp.2004.42.1.27 Abstract PDF We investigated the optimal culture conditions for Cryptosporidium muris in a human stomach adenocarcinoma (AGS) cell line by determining the effects of medium pH and of selected supplements on the development of C. muris. The optimum pH of the culture medium required for the development of C. muris was determined to be 6.6. The number of parasites significantly increased during cultivation for 72 hr (p < 0.05) at this level. On the other hand, numbers decreased linearly after 24 hr of incubation at pH 7.5. When cultured in different concentrations of serum, C. muris in media containing 5% FBS induced 4-7 times more parasites than in 1% or 10% serum. Of the six medium supplements examined, only 1 mM pyruvate enhanced the number of C. muris in vitro. Transmission electron microscopic observation showed the developmental stages of C. muris in the cytoplasm of the cells, not in an extracytoplasmic location. The growth of C. muris in AGS cells provides a means of investigating its biological characteristics and of testing its response to therapeutic agents. However, a more optimized culture system is needed for the recovery of oocysts on a large scale in vitro. Citations Citations to this article as recorded by A Scoping Review of Changing Climate and Weather Connections to Waterborne Pathogens in Coastal Environments Bailey M. Magers, Kyle D. Brumfield, Moiz Usmani, Rita R. Colwell, Antarpreet S. Jutla Global Change Biology.2025;[Epub] CrossRef Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans Janka Melicherová, Lada Hofmannová, Andrea Valigurová European Journal of Protistology.2018; 62: 101. CrossRef The fine structure of sexual stage development and sporogony ofCryptosporidium parvumin cell-free culture HEBATALLA M. ALDEYARBI, PANAGIOTIS KARANIS Parasitology.2016; 143(6): 749. CrossRef Morphology and infectivity of virus that persistently caused infection in an AGS cell line Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno, Tomohiko Takasaki, Kouichi Sano Medical Molecular Morphology.2011; 44(4): 213. CrossRef Evolution of Cryptosporidium in vitro culture P. Karanis, H.M. Aldeyarbi International Journal for Parasitology.2011; 41(12): 1231. CrossRef Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells Liang Wu, Sheng-xia Chen, Xu-gan Jiang, Yu-juan Shen, Zhao-xi Lu, Guo-hua Tu, Xing-li Fu, Jian-ping Cao Parasitology Research.2009; 105(5): 1419. CrossRef 8,771 View 77 Download Crossref