Parasites, Hosts and Diseases

"IFN-γ"

Brief Communications

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii: Francine C. Paim, Aleksandro S. Da Silva, Carlos Breno V. Paim, Raqueli T. Fran?a, M?rcio M. Costa, Marta M. M. F. Duarte, Manuela B. Sangoi, Rafael N. Moresco, Silvia G. Monteiro, Sonia Terezinha A. Lopes; Korean J Parasitol 2013;51(1):133-137.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.133

Abstract
PDF

This study aimed to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NO_x) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-γ, TNF-α, IL-1, IL-6, and NO_x. Cytokines were assessed by ELISA quantitative sandwich technique, and NO_x was measured by the modified Griess method. Cytokine levels (IFN-γ, TNF-α, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NO_x were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

Citations

Citations to this article as recorded by

Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with Leishmania infantum Infection
Luis Álvarez, Pablo-Jesús Marín-García, Pilar Rentero-Garrido, Lola Llobat
Veterinary Sciences.2022; 9(11): 608. CrossRef
Disease severity and blood cytokine concentrations in dogs with natural Babesia rossi infection
Andrew Leisewitz, Amelia Goddard, Jill De Gier, Jessica Van Engelshoven, Sarah Clift, Peter Thompson, Johan P. Schoeman
Parasite Immunology.2019;[Epub] CrossRef
Cytokine Concentrations Measured by Multiplex Assays in Canine Peripheral Blood Samples
Kristen R. Richter, Amirah N. Nasr, Angela M. Mexas
Veterinary Pathology.2018; 55(1): 53. CrossRef
Relation of antioxidant status at admission and disease severity and outcome in dogs naturally infected with Babesia canis canis
Martina Crnogaj, José Joaquin Cerón, Iva Šmit, Ivana Kiš, Jelena Gotić, Mirna Brkljačić, Vesna Matijatko, Camila Peres Rubio, Nada Kučer, Vladimir Mrljak
BMC Veterinary Research.2017;[Epub] CrossRef
Serum biochemistry profile, inflammatory cytokines, adipokines and cardiovascular findings in obese dogs
Diego Piantedosi, Antonio Di Loria, Jacopo Guccione, Angela De Rosa, Silvia Fabbri, Laura Cortese, Sergio Carta, Paolo Ciaramella
The Veterinary Journal.2016; 216: 72. CrossRef
Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis
Amelia Goddard, Andrew L. Leisewitz, Mads Kjelgaard-Hansen, Annemarie T. Kristensen, Johan P. Schoeman, Michelle L. Baker
PLOS ONE.2016; 11(3): e0150113. CrossRef
Canine rangeliosis due to Rangelia vitalii: From first report in Brazil in 1910 to current day – A review
Raqueli T. França, Aleksandro S. Da Silva, Alexandre P. Loretti, Cinthia M. Mazzanti, Sonia T.A. Lopes
Ticks and Tick-borne Diseases.2014; 5(5): 466. CrossRef
Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis
Wojciech Zygner, Olga Gójska-Zygner, Piotr Bąska, Ewa Długosz
Parasitology Research.2014; 113(4): 1499. CrossRef
Increase nitric oxide and oxidative stress in dogs experimentally infected by Ehrlichia canis: Effect on the pathogenesis of the disease
Aleksandro S. Da Silva, Thiago D. Munhoz, Joice L.M. Faria, Giovanni Vargas-Hérnandez, Rosangela Z. Machado, Taís C. Almeida, Rafael N. Moresco, Lenita M. Stefani, Mirela Tinucci-Costa
Veterinary Microbiology.2013; 164(3-4): 366. CrossRef

9,722 View
88 Download
Crossref

Suppression of CD4⁺ T-Cells in the Spleen of Mice Infected with Toxoplasma gondii KI-1 Tachyzoites: Won-Hee Kim, Eun-Hee Shin, Jae-Lip Kim, Seung-Young Yu, Bong-Kwang Jung, Jong-Yil Chai; Korean J Parasitol 2010;48(4):325-329.
Published online December 16, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.4.325

Abstract
PDF

Toxoplasma gondii KI-1, a recent new isolate from Korea, shows similar pathogenicity and infectivity to mice compared to the virulent RH strain. To understand characteristics of host immunity, including immune enhancement or suppression, we investigated proliferative responses and phenotypes of spleen cells. In addition, kinetics of IFN-γ, a Th1 cytokine, was examined in BALB/c mice up to day 6 post-infection (PI). Intraperitoneal injection of mice with 10³ KI-1 tachyzoites induced significant decreases (P < 0.05) in proliferative responses of spleen cells. This occurred at days 2-6 PI even when concanavalin A (con A) was added and when stimulated with KI-1 antigen, suggesting suppression of the immunity. CD4⁺ T-cells decreased markedly at day 2 PI (P < 0.05), whereas CD8⁺ T-cells, NK cells, and macrophages did not show significant changes, except a slight, but significant, increase of CD8⁺ T-cells at day 6 PI. The capacity of splenocytes to produce IFN-γ by con A stimulation dropped significantly at days 2-6 PI. These results demonstrate that intraperitoneal injection of KI-1 tachyzoites can induce immunosuppression during the early stage of infection, as revealed by the decrease of CD4⁺ T-cells and IFN-γ.

Citations

Citations to this article as recorded by

Exploring the potential of Toxoplasma gondii in drug development and as a delivery system
Chanjin Yoon, Yu Seong Ham, Woo Jin Gil, Chul-Su Yang
Experimental & Molecular Medicine.2024; 56(2): 289. CrossRef
Toxoplasma gondii macrophage migration inhibitory factor shows anti– Mycobacterium tuberculosis potential via AZIN1/STAT1 interaction
Chanjin Yoon, Hyo Keun Kim, Yu Seong Ham, Woo Jin Gil, Seok-Jun Mun, Euni Cho, Jae-Min Yuk, Chul-Su Yang
Science Advances.2024;[Epub] CrossRef
The effects of toxoplasmosis on sex ratio at birth
Jaroslav Flegr, Šárka Kaňková
Early Human Development.2020; 141: 104874. CrossRef
Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice
Xiucai Xu, Tingting Liu, Aimei Zhang, Xingxing Huo, Qingli Luo, Zhaowu Chen, Li Yu, Qing Li, Lili Liu, Zhao-rong Lun, Jilong Shen, J. H. Adams
Infection and Immunity.2012; 80(6): 2121. CrossRef

8,133 View
63 Download
Crossref

Original Articles

Aureobasidium-Derived Soluble Branched (1,3-1,6) β-Glucan (Sophy β-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice: Lalani Yatawara, Susiji Wickramasinghe, Mitsuru Nagataki, Misa Takamoto, Haruka Nomura, Yasunori Ikeue, Yoshiya Watanabe, Takeshi Agatsuma; Korean J Parasitol 2009;47(4):345-351.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.345

Abstract
PDF

The β-glucans derived from yeast cell walls have been reported for having many immunomodulatory activities in vivo and in vitro. In this study, Aureobasidium-derived soluble branched (1,3-1,6) β-glucan (Sophy β-glucan) was checked for natural killer (NK) activity and for the production of IFN-γ and IL-4 in Leishmania amazonensis infection. The main experiment was performed with a group of female C57BL/6 and BALB/c mice, orally supplemented with 5% of Sophy β-glucan and infected with promastogotes of L. amazonensis (1 × 10⁷) into the footpad. Increase in the footpad thickness with time was observed in BALB/c mice in spite of the oral Sophy β-glucan supplement, but it was less in C57BL/6 mice. The difference in overall mean footpad thickness between 'infection only' versus 'infection + glucan' groups was statistically significant (P < 0.001). High NK activity in C57BL/6 than BALB/c mice was observed in 'glucan only' group compared to the control group and also in 'infection + glucan' group compared to 'infection only' group. The difference in the NK activity among these groups was significant (P < 0.05). The IFN-γ level increased at weeks 7 and 8 post-infection in C57BL/6 mice and was significantly high in 'infection + glucan' group compared to the 'infection only' group (P < 0.05). IL-4 levels did not increase up to detectable levels throughout the study. The results led a conclusion that Sophy β-glucan enhances NK activity and cellular immunity in L. amazonensis-infected mice.

Citations

Citations to this article as recorded by

Modulation of neutrophil-to-lymphocyte ratio and gut microbiome balance in astronauts: potential benefits of novel beta-glucans during space missions
Nobunao Ikewaki, Koji Ichiyama, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel J. K. Abraham
Frontiers in Immunology.2025;[Epub] CrossRef
Investigating Therapeutic Potential of Beta‐Glucan on Experimental Toxoplasmosis in Immunocompetent and Immunocompromised Mice: In Silico and in Vivo Studies
Asmaa A. Aboul‐Fotouh, Faten A. Elfeky, Nahed R. Omar, Tarek Aboushousha, Eman S. El‐Wakil
Chemistry & Biodiversity.2025;[Epub] CrossRef
Botryosphaeran, [(1 → 3)(1 → 6)-β-D-glucan], induces apoptosis-like death in promastigotes of Leishmania amazonensis, and exerts a leishmanicidal effect on infected macrophages by activating NF-kB and producing pro-inflammatory molecules
Amanda Cristina Machado Carloto, Bruna Taciane da Silva Bortoleti, Ana Carolina Jacob Rodrigues, Taylon Felipe Silva, Fernanda Tomiotto-Pellissier, Danielle Lazarin Bidóia, Manoela Daiele Gonçalves, João Paulo Assolini, Robert F.H. Dekker, Aneli M. Barbos
Chemico-Biological Interactions.2022; 351: 109713. CrossRef
Leishmanicidal activity of fungal bioproducts: A systematic review
Márcio Thomaz dos Santos Varjão, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa, Magna Suzana Alexandre-Moreira, Aline Cavalcanti de Queiroz
Fungal Biology Reviews.2022; 40: 91. CrossRef
β-glucans: wide-spectrum immune-balancing food-supplement-based enteric (β-WIFE) vaccine adjuvant approach to COVID-19
Nobunao Ikewaki, Masaru Iwasaki, Gene Kurosawa, Kosagi-Sharaf Rao, Johant Lakey-Beitia, Senthilkumar Preethy, Samuel JK Abraham
Human Vaccines & Immunotherapeutics.2021; 17(8): 2808. CrossRef
β‑glucan vaccine adjuvant approach for cancer treatment through immune enhancement (B‑VACCIEN) in specific immunocompromised populations (Review)
Nobunao Ikewaki, Vidyasagar Dedeepiya, Kadalraja Raghavan, Kosagi-Sharaf Rao, Suryaprakash Vaddi, Hiroshi Osawa, Tomohiko Kisaka, Gene Kurosawa, Subramaniam Srinivasan, Seydunganallu Kumar, Rajappa Senthilkumar, Masaru Iwasaki, Senthilkumar Preethy, Samue
Oncology Reports.2021;[Epub] CrossRef
Role of Immune Dysregulation in Increased Mortality Among a Specific Subset of COVID-19 Patients and Immune-Enhancement Strategies for Combatting Through Nutritional Supplements
Kosagi-Sharaf Rao, Vaddi Suryaprakash, Rajappa Senthilkumar, Senthilkumar Preethy, Shojiro Katoh, Nobunao Ikewaki, Samuel J. K. Abraham
Frontiers in Immunology.2020;[Epub] CrossRef
Coagulopathy associated with COVID-19 – Perspectives & Preventive strategies using a biological response modifier Glucan
Nobunao Ikewaki, Kosagi-Sharaf Rao, Armando Durant Archibold, Masaru Iwasaki, Rajappa Senthilkumar, Senthilkumar Preethy, Shojiro Katoh, Samuel J. K. Abraham
Thrombosis Journal.2020;[Epub] CrossRef
The preventable efficacy of β-glucan against leptospirosis
Jiaqi Wang, Zhao Jin, Wenlong Zhang, Xufeng Xie, Ning Song, Tianbao Lv, Dianjun Wu, Yongguo Cao, Elizabeth Angelica Leme Martins
PLOS Neglected Tropical Diseases.2019; 13(11): e0007789. CrossRef
β-Glucan and parasites
V. Vetvicka, R. Fernandez-Botran
Helminthologia.2018; 55(3): 177. CrossRef
Effect of Immune-Enhancing Enteral Nutrition Enriched with or without Beta-Glucan on Immunomodulation in Critically Ill Patients
Jae Lee, Young Kim, Young Lee, Hyeon Ahn, Minjoo Kim, Minkyung Kim, Min Cho, Younsoo Cho, Jong Lee
Nutrients.2016; 8(6): 336. CrossRef
Effect of the Medicinal Agaricus blazei Murill-Based Mushroom Extract, AndoSanTM, on Symptoms, Fatigue and Quality of Life in Patients with Crohn’s Disease in a Randomized Single-Blinded Placebo Controlled Study
Stig Palm Therkelsen, Geir Hetland, Torstein Lyberg, Idar Lygren, Egil Johnson, John Green
PLOS ONE.2016; 11(7): e0159288. CrossRef
Evaluation of adjuvant activity of fractions derived from Agaricus blazei, when in association with the recombinant LiHyp1 protein, to protect against visceral leishmaniasis
Nathália Cristina de Jesus Pereira, Wiliam César Bento Régis, Lourena Emanuele Costa, Jamil Silvano de Oliveira, Alanna Gomes da Silva, Vivian Tamietti Martins, Mariana Costa Duarte, José Roberto Rodrigues de Souza, Paula Sousa Lage, Mônica Santos Schneid
Experimental Parasitology.2015; 153: 180. CrossRef
Oral administration of the Aureobasidium pullulans-derived β-glucan effectively prevents the development of high fat diet-induced fatty liver in mice
Shiho Aoki, Atsushi Iwai, Koji Kawata, Daisuke Muramatsu, Hirofumi Uchiyama, Mitsuyasu Okabe, Masahiro Ikesue, Naoyoshi Maeda, Toshimitsu Uede
Scientific Reports.2015;[Epub] CrossRef
Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice
Shiho Aoki, Atsushi Iwai, Koji Kawata, Daisuke Muramatsu, Hirofumi Uchiyama, Mitsuyasu Okabe, Masahiro Ikesue, Naoyoshi Maeda, Toshimitsu Uede
Journal of Functional Foods.2015; 18: 22. CrossRef
Stimulation of Macrophages with the β-Glucan Produced by Aureobasidium pullulans Promotes the Secretion of Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)
Koji Kawata, Atsushi Iwai, Daisuke Muramatsu, Shiho Aoki, Hirofumi Uchiyama, Mitsuyasu Okabe, Sumio Hayakawa, Akinori Takaoka, Tadaaki Miyazaki, Yong J. Lee
PLOS ONE.2015; 10(4): e0124809. CrossRef
Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH
Fabiana Rodrigues de Santana, Cidéli de Paula Coelho, Thayná Neves Cardoso, Elizabeth Cristina Perez Hurtado, Nilson Roberti Benites, Marcia Dalastra Laurenti, Leoni Villano Bonamin
Homeopathy.2014; 103(04): 264. CrossRef
Improved β-glucan yield using an Aureobasidium pullulans M-2 mutant strain in a 200-L pilot scale fermentor targeting industrial mass production
Naoyuki Moriya, Yukiko Moriya, Hideo Nomura, Kisato Kusano, Yukoh Asada, Hirofumi Uchiyama, Enoch Y. Park, Mitsuyasu Okabe
Biotechnology and Bioprocess Engineering.2013; 18(6): 1083. CrossRef
Fermentation of non-sterilized fish biomass with a mixed culture of film-forming yeasts and lactobacilli and its effect on innate and adaptive immunity in mice
Shigeaki Inoue, Kyoko Suzuki-Utsunomiya, Yukako Komori, Akemi Kamijo, Isao Yumura, Koudai Tanabe, Ayumi Miyawaki, Kunimasa Koga
Journal of Bioscience and Bioengineering.2013; 116(6): 682. CrossRef
A small scale study on the effects of oral administration of the β-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves
Hirofumi Uchiyama, Atsushi Iwai, Yukoh Asada, Daisuke Muramatsu, Shiho Aoki, Koji Kawata, Kisato Kusano, Koji Nagashima, Daisuke Yasokawa, Mitsuyasu Okabe, Tadaaki Miyazaki
BMC Research Notes.2012;[Epub] CrossRef
β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice
Daisuke Muramatsu, Atsushi Iwai, Shiho Aoki, Hirohumi Uchiyama, Koji Kawata, Yosuke Nakayama, Yasuhiro Nikawa, Kisato Kusano, Mitsuyasu Okabe, Tadaaki Miyazaki, Kevin Harrod
PLoS ONE.2012; 7(7): e41399. CrossRef
Effects of Sophy β-glucan on growth performance, carcass traits, meat composition, and immunological responses of Peking ducks
X.Y. Tang, J.S. Gao, F. Yuan, W.X. Zhang, Y.J. Shao, F. Sakurai, Z.D. Li
Poultry Science.2011; 90(4): 737. CrossRef

9,062 View
105 Download
Crossref

Single Nucleotide Polymorphisms of Cytokine Genes are Associated with Fibrosis of the Intrahepatic Bile Duct Wall in Human Clonorchiasis: Byung-Suk Chung, Jeong-Keun Lee, Min-Ho Choi, Myoung Hee Park, Dongil Choi, Sung-Tae Hong; Korean J Parasitol 2009;47(2):145-151.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.145

Abstract
PDF

This study examined the association of cytokine gene polymorphisms with intrahepatic bile duct wall fibrosis in human clonorchiasis. A total of 240 residents in Heilongjiang, China underwent ultrasonography, blood sampling, and stool examination. Single nucleotide polymorphism (SNP) sites for IFN-γ (+874 T/A), IL-10 (-1,082 G/A, -819 C/T, -592 C/A), TNF-α (-308 G/A), and TGF-β1 (codon 10 T/C, codon 25 G/C) genes were observed with the TaqMan allelic discrimination assay. No significant correlation was observed between individual cytokine gene polymorphisms and intrahepatic duct dilatation (IHDD). Among individuals with clonorchiasis of moderate intensity, the incidence of IHDD was high in those with IFN-γ intermediate-producing genotype, +874AT (80.0%, P = 0.177), and in those with TNF-α low-producing genotype, -308GG (63.0%, P = 0.148). According to the combination of IFN-γ and TNF-α genotypes, the risks for IHDD could be stratified into high (intermediate-producing IFN-γ and low producing TNF-α), moderate, and low (low-producing IFN-γ and high producing TNF-α) risk groups. The incidence of IHDD was significantly different among these groups (P = 0.022): 88.9% (odds ratio, OR = 24.0) in high, 56.5% (OR = 3.9) in moderate, and 25.0% (OR = 1) in low risk groups. SNP of IFN-γ and TNF-α genes may contribute to the modulation of fibrosis in the intrahepatic bile duct wall in clonorchiasis patients.

Citations

Citations to this article as recorded by

Risk Factors of Clonorchis sinensis Human Infections in Endemic Areas, Haman-Gun, Republic of Korea: A Case-Control Study
Sang-Eun Lee, Hee-Eun Shin, Myoung-Ro Lee, Yang-Hee Kim, Shin-Hyeong Cho, Jung-Won Ju
The Korean Journal of Parasitology.2020; 58(6): 647. CrossRef
The impact of cytokine gene polymorphisms on Epstein–Barr virus infection outcome in pediatric liver transplant recipients
Beata Kasztelewicz, Irena Jankowska, Joanna Pawłowska, Joanna Teisseyre, Katarzyna Dzierżanowska-Fangrat
Journal of Clinical Virology.2012; 55(3): 226. CrossRef
Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies
Ling Zeng, Wei Gu, Kehong Chen, Dongpo Jiang, Lianyang Zhang, Dingyuan Du, Ping Hu, Qing Liu, Suna Huang, Jianxin Jiang
Critical Care.2009;[Epub] CrossRef

8,023 View
68 Download
Crossref

Lambs Infected with UV-Attenuated Sporocysts of Sarcocystis ovicanis Produced Abnormal Sarcocysts and Induced Protective Immunity against a Challenge Infection: Abdel-Azeem Abdel-Baki, Gamal Allam, Thabet Sakran, El-Mahy El-Malah; Korean J Parasitol 2009;47(2):131-138.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.131

Abstract
PDF

The present study surveyed the prevalence of natural infection of the sheep esphagus muscle with sarcocysts of Sarcocystis ovicanis and examined induction of protective immunity using UV-attenuated sporocysts. The overall prevalence of natural infection of the sheep was 95%. Infectivity of the collected sarcocysts was confirmed by shedding of sporulated oocysts after feeding infected esophageal tissues to dogs. To induce protective immunity, lambs were immunized 3 times (once a week) with 1.5 × 10⁴ sporocysts exposed to UV-light for 30 min (UV-30 group) or 60 (UV-60 group) min and then challenged with 1.5 × 10⁴ normal sporocysts at the 3rd week post the 1st vaccination. These lambs showed high survival and less clinical signs of sarcocystosis than normal infected lambs. The attenuated sporocysts produced abnormal cysts; small in size and detached from the muscle fiber. These abnormalities were more obvious in UV-60 group than UV-30 group. Also, the IFN-γ level and lymphocyte percentage were increased while the total leukocyte count was decreased in the UV-60 group compared with other groups. The high level of IFN-γ may be an evidence for the induction of Th1 responses which may have protective effect against a challenge infection.

Citations

Citations to this article as recorded by

Investigation into the potential of using UV-treated sporulated oocysts of Eimeria tenella as a local solution to immunization of chickens against caecal coccidiosis
Samir Djemai, Abdeslam Mekroud, Geoff Hide, Daoudi Khelifi, Inès Bellil
Journal of Parasitic Diseases.2023; 47(2): 238. CrossRef
Morphometric Study of Microscopic Cysts of Sarcocystis Sp. in Sheep Carcasses
As. Dahmani, M. Aissi, S. Zenia, K. Harhoura, R. Kadour, A. Saadi
Folia Veterinaria.2020; 64(3): 38. CrossRef
An update on sarcocystosis in one-humped camels (Camelus dromedarius)
Muhammad A. Saeed, Jane L. Vaughan, Abdul Jabbar
Parasitology.2018; 145(11): 1367. CrossRef
Immunoprotective responses against murine sarcocystosis by β - Irradiated sporocysts
Mahmoud Abdel-Latif, Thabet Sakran, Heba M. Abdel-Haleem, Mostafa F. Eissa, Shrouk E. Al-Sayed
Experimental Parasitology.2018; 191: 73. CrossRef

9,256 View
92 Download
Crossref

IL-4 Independent Nuclear Translocalization of STAT6 in HeLa Cells by Entry of Toxoplasma gondii: Hye-Jin Ahn, Ji Yeon Kim, Ho-Woo Nam; Korean J Parasitol 2009;47(2):117-124.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.117

Abstract
PDF

Toxoplasma gondii provokes rapid and sustained nuclear translocation of the signal transducer and activator of transcription 6 (STAT6) in HeLa cells. We observed activation of STAT6 as early as 2 hr after infection with T. gondii by the nuclear translocation of fluorescence expressed from exogenously transfected pDsRed2-STAT6 plasmid and by the detection of phosphotyrosine-STAT6 in Western blot. STAT6 activation occurred only by infection with live tachyzoites but not by co-culture with killed tachyzoites or soluble T. gondii extracts. STAT6 phosphorylation was inhibited by small interfering RNA of STAT6 (siSTAT6). In view of the fact that STAT6 is a central mediator of IL-4 induced gene expression, activation of STAT6 by T. gondii infection resembles that infected host cells has been stimulated by IL-4 treatment. STAT1 was affected to increase the transcription and expression by the treatment of siSTAT6. STAT6 activation was not affected by any excess SOCS's whereas that with IL-4 was inhibited by SOCS-1 and SOCS-3. T. gondii infection induced Eotaxin-3 gene expression which was reduced by IFN-γ. These results demonstrate that T. gondii exploits host STAT6 to take away various harmful reactions by IFN-γ. This shows, for the first time, IL-4-like action by T. gondii infection modulates microbicidal action by IFN-γ in infected cells.

Citations

Citations to this article as recorded by

Nuclear Localization and Cleavage of STAT6 Is Induced by Kaposi’s Sarcoma-Associated Herpesvirus for Viral Latency
Chong Wang, Caixia Zhu, Fang Wei, Shujun Gao, Liming Zhang, Yuhong Li, Yanling Feng, Yin Tong, Jianqing Xu, Bin Wang, Zhenghong Yuan, Erle S. Robertson, Qiliang Cai, Paul D. Ling
PLOS Pathogens.2017; 13(1): e1006124. CrossRef
SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection
Gang Lu, Jian Zhou, Aihua Zhou, Yali Han, Jingjing Guo, Pengxia Song, Huaiyu Zhou, Hua Cong, Ming Hou, Lin Wang, Shenyi He
Parasitology International.2017; 66(5): 596. CrossRef
SAG4 DNA and Peptide Vaccination Provides Partial Protection against T. gondii Infection in BALB/c Mice
Jian Zhou, Lin Wang
Frontiers in Microbiology.2017;[Epub] CrossRef
Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii
Zhaoshou Yang, Hye-Jin Ahn, Young-Hoon Park, Ho-Woo Nam
The Korean Journal of Parasitology.2016; 54(1): 31. CrossRef
Chronic Toxoplasmosis Modulates the Induction of Contact Hypersensitivity by TNCB in Mouse Model
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2015; 53(6): 755. CrossRef
Activation of STAT6 by STING Is Critical for Antiviral Innate Immunity
Huihui Chen, Hui Sun, Fuping You, Wenxiang Sun, Xiang Zhou, Lu Chen, Jing Yang, Yutao Wang, Hong Tang, Yukun Guan, Weiwei Xia, Jun Gu, Hiroki Ishikawa, Delia Gutman, Glen Barber, Zhihai Qin, Zhengfan Jiang
Cell.2011; 147(2): 436. CrossRef
Deactivation of STAT6 through Serine 707 Phosphorylation by JNK
Takashi Shirakawa, Yoshinori Kawazoe, Tomoko Tsujikawa, Dongju Jung, Shin-ichi Sato, Motonari Uesugi
Journal of Biological Chemistry.2011; 286(5): 4003. CrossRef
Coactivator P100 Protein Enhances STAT6‐Dependent Transcriptional Activation But Has No Effect on STAT1‐Mediated Gene Transcription
Xinting Wang, Xin Liu, Jianfei Fang, Yanxin Lu, Jinyan He, Xuyang Yao, Zhi Yao, Jie Yang
The Anatomical Record.2010; 293(6): 1010. CrossRef
Toxoplasma Rhoptry Protein 16 (ROP16) Subverts Host Function by Direct Tyrosine Phosphorylation of STAT6
Yi-Ching Ong, Michael L. Reese, John C. Boothroyd
Journal of Biological Chemistry.2010; 285(37): 28731. CrossRef
STAT6 activation by Toxoplasma gondii infection induces the expression of Th2 C-C chemokine ligands and B clade serine protease inhibitors in macrophage
Hye-Jin Ahn, Ji Yeon Kim, Kyung-Ju Ryu, Ho-Woo Nam
Parasitology Research.2009; 105(5): 1445. CrossRef

11,041 View
101 Download
Crossref

Sequential analysis of cell differentials and IFN-γ production of splenocytes from mice infected with Toxoplasma gondii: Young-Ha Lee, Dae-Whan Shin, Lloyd H. Kasper; Korean J Parasitol 2000;38(2):85-90.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.85

Abstract
PDF

To assess the relationship between the changes of cellular components and the production of Th1 cytokine in the immune tissue, inbred C57BL/6 mice were orally infected with 40 cysts of 76K strain of Toxoplasma gondii. The sequential change of cell differentials and IFN-γ production of splenocytes were analyzed by Diff-Quik stain and RT-PCR. There were no significant proportional changes of cellular components of splenocytes until day 4 postinfection (PI) as compared to those of day 0, and the relative percentage of macrophages and neutrophils/eosinophils increased significantly (p<0.01) thereafter. The expression of IFN-γ mRNA of CD3^- cells was observed from day 1 PI at a low level. However, IFN-γ production of CD3⁺ cells increased significantly from day 4 PI (p<0.01) which progressively increased thereafter. These findings provide the relative percentages of granulocytes and macrophages were increased in conjunction with increase of total number of splenocytes after oral infection with T. gondii in the susceptible murine hosts, and lymphocytes were the major cellular components and the important source of IFN-γ.

Citations

Citations to this article as recorded by

Oral Tolerization with Cardiac Myosin Peptide (614–629) Ameliorates Experimental Autoimmune Myocarditis: Role of Stat 6 Genes in BALB/CJ Mice
Patricia A. Gonnella, Pedro J. Del Nido, Francis X. McGowan
Journal of Clinical Immunology.2009; 29(4): 434. CrossRef
T cell phenotype and intracellular IFN-γ production in peritoneal exudate cells and gut intraepithelial lymphocytes during acute Toxoplasma gondii infection in mice
Young-Ha Lee, Dae-Whan Shin
The Korean Journal of Parasitology.2002; 40(3): 119. CrossRef

8,180 View
82 Download
Crossref

Brief Communication

Changes of splenocyte IFN-γ mRNA synthesis in rats infected with Paragonimus westermani: Jun Kyong Cho, Hye Soo Kwon, Kyoung Hwan Joo, Joon Sang Lee, Sung-Weon Cho; Korean J Parasitol 1999;37(4):285-287.
Published online December 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.4.285

Abstract
PDF

Changes in the expression level of splenocyte IFN-γ mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of IFN-γ had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with P. westermani, the levels of IFN-γ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.

Citations

Citations to this article as recorded by

Adjuvant effect of Korean mistletoe lectin on mucosal immunity induction following intranasal immunization with hemagglutinin antigen
Jin Hyuk Jung, Young Hoon Kim, Tae Jun Song, Hyosun An, Kyu Dae Kim, In Bo Kim, Taek Joon Yoon, Jong Bae Kim
Food Science and Biotechnology.2011; 20(3): 629. CrossRef