Parasites, Hosts and Diseases

"LAMP"

Original Article

A LAMP-SNP Assay Detecting C580Y Mutation in Pfkelch13 Gene from Clinically Dried Blood Spot Samples: Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat; Korean J Parasitol 2021;59(1):15-22.
Published online February 19, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.1.15

Artemisinin resistance (ART) has been confirmed in Greater Mekong Sub-region countries. Currently, C580Y mutation on Pfkelch13 gene is known as the molecular marker for the detection of ART. Rapid and accurate detection of ART in field study is essential to guide malaria containment and elimination interventions. A simple method for collection of malaria-infected blood is to spot the blood on filter paper and is fast and easy for transportation and storage in the field study. This study aims to evaluate LAMP-SNP assay for C580Y mutation detection by introducing an extra mismatched nucleotide at the 3’ end of the FIP primer. The LAMP-SNP assay was performed in a water bath held at a temperature of 56°C for 45 min. LAMP-SNP products were interpreted by both gel-electrophoresis and HNB-visualized changes in color. The method was then tested with 120 P. falciparum DNA from dried blood spot samples. In comparing the LAMP-SNP assay results with those from DNA sequencing of the clinical samples, the 2 results fully agreed to detect C580Y. The sensitivity and specificity of the LAMP-SNP assay showed 100%. There were no cross-reactions with other Plasmodium species and other Pfkelch13 mutations. The LAMP-SNP assay performed in this study was rapid, reliable, and useful in detecting artemisinin resistance in the field study.

Citations

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Colorimetric loop-mediated isothermal amplification (cLAMP) assay for the genotyping of a thrombophilia genetic risk factor, MTHFR (C677T)
Reham Altwayan, Huseyin Tombuloglu, Moneerah Alsaeed, Abdulrahman Alhusil
Analytical Methods.2025; 17(21): 4432. CrossRef
Advancing artemisinin resistance monitoring using a high sensitivity ddPCR assay for Pfkelch13 mutation detection in Asia
Suttipat Srisutham, Aungkana Saejeng, Nardlada Khantikul, Rungniran Sugaram, Raweewan Sangsri, Arjen M. Dondorp, Nicholas P. J. Day, Mallika Imwong
Scientific Reports.2025;[Epub] CrossRef
Loop-mediated isothermal amplification (LAMP): A promising tool for rapid, point-of-care diagnosis of parasitic diseases in low-income countries
Kevin Polpitiya, Madhavi Hewadikaram
Molecular and Biochemical Parasitology.2025; 264: 111704. CrossRef
A Biotinylated cpFIT-PNA Platform for the Facile Detection of Drug Resistance to Artemisinin in Plasmodium falciparum
Odelia Tepper, Daniel H. Appella, Hongchao Zheng, Ron Dzikowski, Eylon Yavin
ACS Sensors.2024; 9(3): 1458. CrossRef
Interference reduction isothermal nucleic acid amplification strategy for COVID-19 variant detection
Guodong Li, Chung-Nga Ko, Zikang Wang, Feng Chen, Wanhe Wang, Dik-Lung Ma, Chung-Hang Leung
Sensors and Actuators B: Chemical.2023; 377: 133006. CrossRef
Super-assembly of integrated gold magnetic assay with loop-mediated isothermal amplification for point-of-care testing
Jianping Liang, Jie Zeng, Xiaojuan Huang, Tengteng Zhu, Yonglong Gong, Chen Dong, Xiangrong Wang, Lingzhi Zhao, Lei Xie, Kang Liang, Qiongxiang Tan, Yali Cui, Biao Kong, Wenli Hui
Nano Research.2023; 16(1): 1242. CrossRef
Engineered Biosensors for Diagnosing Multidrug Resistance in Microbial and Malignant Cells
Niharika G. Jha, Daphika S. Dkhar, Sumit K. Singh, Shweta J. Malode, Nagaraj P. Shetti, Pranjal Chandra
Biosensors.2023; 13(2): 235. CrossRef
Application of loop-mediated isothermal amplification combined with lateral flow assay visualization of Plasmodium falciparum kelch 13 C580Y mutation for artemisinin resistance detection in clinical samples
Wannida Sanmoung, Nongyao Sawangjaroen, Suwannee Jitueakul, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat, Mallika Imwong
Acta Tropica.2023; 246: 106998. CrossRef
Visual single nucleotide polymorphism (SNP) detection for ALDH2 genotyping based on multiplex ligation probe amplification (MLPA) and lateral flow assay
Dan Yin, Xiaolan Li, Li Mai, Ruxin Wang, Sitian Tang, Liyi Hu
Microchemical Journal.2023; 194: 109329. CrossRef
Application of multiple binding sites for LAMP primers across P. falciparum genome improves detection of the parasite from whole blood samples
Cavin Mgawe, Clement Shilluli, Steven Nyanjom, Eddy Odari, Jacqueline C. Linnes, Bernard N. Kanoi, Jesse Gitaka, Lucy Ochola
Frontiers in Malaria.2023;[Epub] CrossRef
Single nucleotide polymorphism genotyping of ALDH2 gene based on asymmetric PCR and fluorescent probe-mediated melting curves
Limei Zhang, Dan Liu, Baolin Li, Jingling Xie, Jinbo Liu, Zhang Zhang
Analytical Biochemistry.2022; 642: 114509. CrossRef
Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study
Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
The Korean Journal of Parasitology.2022; 60(1): 15. CrossRef
Current Epidemiological Characteristics of Imported Malaria, Vector Control Status and Malaria Elimination Prospects in the Gulf Cooperation Council (GCC) Countries
Jamshaid Iqbal, Suhail Ahmad, Ali Sher, Mohammad Al-Awadhi
Microorganisms.2021; 9(7): 1431. CrossRef

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Brief Communications

Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea): Su-Min Song, Dinzouna-Boutamba Sylvatrie-Danne, So-Young Joo, Yun Kyung Shin, Hak Sun Yu, Yong-Seok Lee, Ji-Eon Jung, Noboru Inoue, Won Kee Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(3):305-310.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.305

Abstract
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Ascidian soft tunic syndrome (AsSTS) caused by Azumiobodo hoyamushi (A. hoyamushi) is a serious aquaculture problem that results in mass mortality of ascidians. Accordingly, the early and accurate detection of A. hoyamushi would contribute substantially to disease management and prevention of transmission. Recently, the loop-mediated isothermal amplification (LAMP) method was adopted for clinical diagnosis of a range of infectious diseases. Here, the authors describe a rapid and efficient LAMP-based method targeting the 18S rDNA gene for detection of A. hoyamushi using ascidian DNA for the diagnosis of AsSTS. A. hoyamushi LAMP assay amplified the DNA of 0.01 parasites per reaction and detected A. hoyamushi in 10 ng of ascidian DNA. To validate A. hoyamushi 18S rDNA LAMP assays, AsSTS-suspected and non-diseased ascidians were examined by microscopy, PCR, and by using the LAMP assay. When PCR was used as a gold standard, the LAMP assay showed good agreement in terms of sensitivity, positive predictive value (PPV), and negative predictive value (NPV). In the present study, a LAMP assay based on directly heat-treated samples was found to be as efficient as DNA extraction using a commercial kit for detecting A. hoyamushi. Taken together, this study shows the devised A. hoyamushi LAMP assay could be used to diagnose AsSTS in a straightforward, sensitive, and specific manner, that it could be used for forecasting, surveillance, and quarantine of AsSTS.

Citations

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Measurement of Tunic Hardness in an Edible Ascidian, Halocynthia roretzi, with Remarks on Soft Tunic Syndrome
Euichi Hirose, Kei Nakayama, Tetsuya Yanagida, Akatsuki Nawata, Shin-Ichi Kitamura
Zoological Science.2018; 35(6): 548. CrossRef

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Recent Situation of Taeniasis in Mongolia (2002-2012): Anu Davaasuren, Temuulen Dorjsuren, Tetsuya Yanagida, Yasuhito Sako, Kazuhiro Nakaya, Abmed Davaajav, Gurbadam Agvaandaram, Tsatsral Enkhbat, Battsetseg Gonchigoo, Nyamkhuu Dulmaa, Gantigmaa Chuluunbaatar, Akira Ito; Korean J Parasitol 2014;52(2):211-214.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.211

Abstract
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Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia species and copro-PCR and loop-mediated isothermal amplification (LAMP) assays were utilized for detection of copro-DNA of 37 fecal samples from taeniasis patients submitted to the Mongolian National Center for Communicable Diseases (NCCD) from 2002 to 2012. In addition, 4 out of 44 calcified cysts in beef kept in formalin since 2003 were evaluated for histopathological confirmation of cattle cysticercosis. All proglottid specimens and stool samples were confirmed to be Taenia saginata by multiplex PCR and by copro-PCR and LAMP, respectively. Cysts collected from cattle were morphologically confirmed to be metacestodes of Taenia species. T. saginata taeniasis was identified from almost all ages from a 2-year-old boy up to a 88-year-old woman and most prominently in 15-29 age group (37%, 74/198) followed by 30-44 age group (34.8%, 69/198 ) from 15 of Mongolia's 21 provinces, while cattle cysticerci were found from 12 provinces. The highest proportion of taeniasis patients was in Ulaanbaatar, the capital of Mongolia.

Citations

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Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia
Sayed Ajmal Qurishi, Hong-Bin Yan, Li Li, John Aeskhaen Ohiolei, Mughees Aizaz Alvi, Lin-Sheng Zhang, Ha Da, Hong-Mei Qiao, Nigus Abebe Shumuye, Bao Hua, Bing-Xin Bai, Wen-Jun Tian, Ju-Mei Xu, Bao-Quan Fu, Wan-Zhong Jia
Parasitology Research.2022; 121(12): 3455. CrossRef
Prevalence of meat-transmitted Taenia and Trichinella parasites in the Far East countries
Yi Liu, Zijian Dong, Jianda Pang, Mingyuan Liu, Xuemin Jin
Parasitology Research.2021; 120(12): 4145. CrossRef
Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny
Parasites & Vectors.2020;[Epub] CrossRef
Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook
Miao-Han Deng, Lan-Yi Zhong, Okanurak Kamolnetr, Yanin Limpanont, Zhi-Yue Lv
Infectious Diseases of Poverty.2019;[Epub] CrossRef
Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
Acta Tropica.2019; 198: 105075. CrossRef
Neurocysticercosis: A case study of a Mongolian traveler who visited China and India with an updated review in Asia
Anu Davaasuren, Abmed Davaajav, Baigalmaa Ukhnaa, Altantsetseg Purvee, Saraa Unurkhaan, Amartuvshin Luvsan, Jenae E. Logan, Akira Ito
Travel Medicine and Infectious Disease.2017; 20: 31. CrossRef
Novel approaches to the diagnosis of Strongyloides stercoralis infection
D. Buonfrate, F. Formenti, F. Perandin, Z. Bisoffi
Clinical Microbiology and Infection.2015; 21(6): 543. CrossRef

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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR: Yanhua Wang, Guangxiang Wang, Delin Zhang, Hong Yin, Meng Wang; Korean J Parasitol 2013;51(5):573-577.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.573

Abstract
PDF

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

Citations

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First report of molecular detection and phylogenetic analysis of Toxoplasma Gondii in soil, water and vegetables from Chandigarh city, India
Divya Rattan, Priya Datta, Devyani Sharma, Chandra Kanta Bhusal, Rakesh Sehgal
Scientific Reports.2025;[Epub] CrossRef
Detection of chronic toxoplasmosis in the brain of mice using loop-mediated isothermal amplification (LAMP) and conventional PCR
Mona K. Hegazy, Nora E. Saleh, Wafaa A. Aboukamar
Experimental Parasitology.2023; 251: 108556. CrossRef
Immune system roles in pathogenesis, prognosis, control, and treatment of Toxoplasma gondii infection
Mohammad Mahdi Jafari, Zahra Azimzadeh Tabrizi, Mohammad Saaid Dayer, Nazanin Atieh Kazemi-Sefat, Mahshid Mohtashamifard, Rahimeh Mohseni, Atefeh Bagheri, Saeed Bahadory, Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar
International Immunopharmacology.2023; 124: 110872. CrossRef
Clinical validation of visual LAMP and qLAMP assays for the rapid detection of Toxoplasma gondii
Zhi Cao, Ke Zhang, Dehua Yin, Qiaoya Zhang, Ying Yu, Jianxin Wen, Hongbo Ni
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
RAA-Cas12a-Tg: A Nucleic Acid Detection System for Toxoplasma gondii Based on CRISPR-Cas12a Combined with Recombinase-Aided Amplification (RAA)
Qiao-Ni Ma, Meng Wang, Lai-Bao Zheng, Zi-Qin Lin, Muhammad Ehsan, Xing-Xing Xiao, Xing-Quan Zhu
Microorganisms.2021; 9(8): 1644. CrossRef
Loop mediated isothermal amplification (LAMP) of Toxoplasma DNA from dried blood spots
Mona K. Hegazy, Soha I. Awad, Nora E. Saleh, Mamdouh M. Hegazy
Experimental Parasitology.2020; 211: 107869. CrossRef
Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection
Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
Infection.2018; 46(3): 303. CrossRef
Molecular detection and phylogenetic analyses ofToxoplasma gondiifrom naturally infected sheep in Northern and Central Tunisia
Mariem Rouatbi, Yosra Amdouni, Safa Amairia, Mohamed R. Rjeibi, Said Sammoudi, Mourad Rekik, Mohamed Gharbi
Veterinary Medicine and Science.2017; 3(1): 22. CrossRef
Comparison of PCR assays targeting the multi-copy targets B1 gene and 529 bp repetitive element for detection of Toxoplasma gondii in swine muscle
Fabrizia Veronesi, Azzurra Santoro, Giovanni Luigi Milardi, Manuela Diaferia, Raffaella Branciari, Dino Miraglia, Attilia Cioffi, Simona Gabrielli, David Ranucci
Food Microbiology.2017; 63: 213. CrossRef
Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran
Amir Abdoli, Abdolhossein Dalimi, Haleh Soltanghoraee, Fatemeh Ghaffarifar
Journal of Parasitic Diseases.2016; 40(4): 1317. CrossRef
Development and application of loop-mediated isothermal amplification assays based on ITS-1 for rapid detection of Toxoplasma gondii in pork
Xunhui Zhuo, Bin Huang, Jiaqing Luo, Haijie Yu, Baolong Yan, Yi Yang, Aifang Du
Veterinary Parasitology.2015; 208(3-4): 246. CrossRef
Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
Quan Liu, Ze-Dong Wang, Si-Yang Huang, Xing-Quan Zhu
Parasites & Vectors.2015;[Epub] CrossRef
Loop-Mediated Isothermal Amplification for Rapid and Semiquantitative Detection of Loa loa Infection
Papa M. Drame, Doran L. Fink, Joseph Kamgno, Jesica A. Herrick, Thomas B. Nutman, M. J. Loeffelholz
Journal of Clinical Microbiology.2014; 52(6): 2071. CrossRef

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Original Article

Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba: Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2013;51(3):269-277.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.269

Abstract
PDF

Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.

Citations

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Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii
Susanna Haapanen, Maarit S. Patrikainen, Seppo Parkkila
Diagnostic Microbiology and Infectious Disease.2023; 107(2): 116014. CrossRef
A simple and visible detection method for the rapid diagnosis of Ustilaginoidea virens in rice seeds by a loop‐mediated isothermal amplification assay
Wei Wang, Hang Yin, Ning Huang, Cuijing Zhu, Yufei Wang, Xintong Qi, Lu Ma, Yunxin Fan, Yao Yu, Hongsheng Zhang, Yongmei Bao
Journal of Phytopathology.2021; 169(6): 369. CrossRef
Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample
Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
Scientific Reports.2021;[Epub] CrossRef
Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis of Plasmodium vivax Malaria in a Tertiary Hospital in Chandigarh, North India
Hargobinder Kaur, Rakesh Sehgal, Devendra Bansal, Ali A. Sultan, Ashish Bhalla, Sunit C. Singhi
The American Journal of Tropical Medicine and Hygiene.2018; 98(5): 1374. CrossRef
Detection of Acanthamoeba spp. in water samples collected from natural water reservoirs, sewages, and pharmaceutical factory drains using LAMP and PCR in China
Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
Science of The Total Environment.2017; 584-585: 489. CrossRef
Water-borne protozoa parasites: The Latin American perspective
Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
International Journal of Hygiene and Environmental Health.2017; 220(5): 783. CrossRef
Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
Indian Journal of Medical Microbiology.2017; 35(1): 90. CrossRef
Acanthamoeba keratitis: improving the Scottish diagnostic service for the rapid molecular detection of Acanthamoeba species
Claire Low Alexander, Michael Coyne, Brian Jones, Deepa Anijeet
Journal of Medical Microbiology .2015; 64(7): 682. CrossRef
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Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
Experimental Biology and Medicine.2014; 239(11): 1443. CrossRef

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Brief Communication

Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification: Jie Li, Peiyuan Wang, Aiguo Zhang, Ping Zhang, Muhamd Alsarakibi, Guoqing Li; Korean J Parasitol 2013;51(2):237-241.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.237

Abstract
PDF

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from 10^-1 to 10^-5 ng/?l for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of 63℃ by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha (EF1α) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.

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Mamta Thakur, Abhishek Mewara, PVM Lakshmi, Sucheta Guleria, Sumeeta Khurana
Diagnostic Microbiology and Infectious Disease.2025; 112(3): 116808. CrossRef
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Parasitologia.2025; 5(2): 25. CrossRef
Real‐time loop‐mediated isothermal amplification (real‐time LAMP) assay for rapid diagnosis of amoebic liver abscess
Sandhya Khunger, Abhishek Mewara, Upninder Kaur, Ajay Duseja, Pallab Ray, Naveen Kalra, Navneet Sharma, Rakesh Sehgal
Tropical Medicine & International Health.2024; 29(2): 104. CrossRef
A colorimetric assay for Neospora caninum utilizing the loop-mediated isothermal amplification technique
Tingting Liu, Kairao Hu, Meiyi Chen, Hongrong Hong, Xi Jiang, Rongsheng Huang, Yiwen Wang, Jing Huang, Xingang Yu, Quan Liu, Zhengkai Wei
Research in Veterinary Science.2024; 179: 105395. CrossRef
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Meysam Khodaparast, Dave Sharley, Stephen Marshall, Travis Beddoe
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Detection of chronic toxoplasmosis in the brain of mice using loop-mediated isothermal amplification (LAMP) and conventional PCR
Mona K. Hegazy, Nora E. Saleh, Wafaa A. Aboukamar
Experimental Parasitology.2023; 251: 108556. CrossRef
A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis
Abhishek Mewara, Sandhya Khunger, Chayan Sharma, Sivanantham Krishnamoorthi, Shreya Singh, Rakesh Yadav, Sumeeta Khurana, Rakesh Sehgal
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Norhamizah Roshidi, Norsyahida Arifin, Francisco Gonzalez Salazar
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Laura F. Lalonde, Vincent Xie, Jenna R. Oakley, Vladislav A. Lobanov
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Loop mediated isothermal amplification (LAMP) of Toxoplasma DNA from dried blood spots
Mona K. Hegazy, Soha I. Awad, Nora E. Saleh, Mamdouh M. Hegazy
Experimental Parasitology.2020; 211: 107869. CrossRef
Sensitive and rapid detection of Ortleppascaris sinensis (Nematoda: Ascaridoidea) by loop-mediated isothermal amplification
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Aongart Mahittikorn, Nipa Thammasonthijarern, Amonrattana Roobthaisong, Ruenruetai Udonsom, Supaluk Popruk, Sukhontha Siri, Hirotake Mori, Yaowalark Sukthana
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Development of a Loop Mediated Isothermal Amplification for Diagnosis ofAscaris lumbricoidesin Fecal Samples
Esther A. Shiraho, Agola L. Eric, Ibrahim N. Mwangi, Geoffrey M. Maina, Joseph M. Kinuthia, Martin W. Mutuku, Robert M. Mugambi, Jackson M. Mwandi, Gerald M. Mkoji
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Jin-Cheol Shin, Alisha Wehdnesday Bernardo Reyes, Sang-Hun Kim, Suk Kim, Hyung-Jin Park, Kyoung-Won Seo, Kun-Ho Song
The Korean Journal of Parasitology.2015; 53(4): 477. CrossRef
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