Parasites, Hosts and Diseases

"Youn-Kyoung Goo"

Brief Communication

Glucose-6-phosphate dehydrogenase variants in Kachin, Myanmar: Zin Moon, Ja Moon Aung, Dorene VanBik, Hae Soo Yun, Sanghyun Lee, Sylvatrie-Danne Dinzouna-Boutamba, Zau Ring, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo; Parasites Hosts Dis 2025;63(4):360-363.
Published online November 19, 2025; DOI: https://doi.org/10.3347/PHD.25053

Abstract
PDF

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked recessive genetic disorder that can cause severe anemia in affected individuals exposed to oxidative stress. This risk is particularly relevant in patients treated with the antimalarial drug primaquine. In Myanmar, primaquine has been widely administered as a Plasmodium vivax malaria treatment; however, prevalence of G6PD deficiency among the population remains insufficiently characterized. This study investigated the prevalence of G6PD variants among various minority ethnic subgroups residing in Kachin State, Myanmar. Blood samples from 440 participants were analyzed; however, the Mahidol variant (G487A) was identified in 21 individuals (4.8%). A major limitation of this study was the absence of G6PD enzyme activity data to confirm whether the Mahidol variant induces G6PD deficiency.

275 View
11 Download

Original Articles

Establishing a Cre/loxP-based genetic manipulation system for Acanthamoeba: Targeted genome editing and stable reporter expression: Ja Moon Aung, So-Young Joo, Byoung-Kuk Na, Seunghyeok Bang, Minsang Shin, Youn-Kyoung Goo, Yeonchul Hong; Parasites Hosts Dis 2025;63(1):25-36.
Published online February 25, 2025; DOI: https://doi.org/10.3347/PHD.24078

Acanthamoeba is an opportunistic pathogen responsible for granulomatous amoebic encephalitis and amoebic keratitis. Despite its clinical significance, effective treatments remain challenging due to a limited understanding of its pathogenic mechanism. This study developed a genetic manipulation system in Acanthamoeba to facilitate gene function and drug screening studies. We applied the Cre/loxP system to integrate the gene encoding the tdTomato fluorescent protein into the genome of Acanthamoeba castellanii via homologous recombination. The polyubiquitin gene and its untranslated regions were identified and verified, after which the tdTomato gene was cloned between the untranslated regions of the polyubiquitin gene. The construct was then introduced into the Acanthamoeba genome using a modified pLPBLP vector containing loxP sites. Cre recombinase was utilized to remove the neomycin resistance cassette flanked by loxP sites, and genetically modified cells were selected by clonal dilution. The integration of the tdTomato gene, confirmed through PCR and fluorescence microscopy, showed stable expression in both trophozoites and cysts without the need for antibiotic selection. We demonstrated the feasibility of antibiotic-free reporter gene expression in Acanthamoeba. The system provides a valuable tool for functional genomics, allowing us to explore gene functions in Acanthamoeba and develop reliable drug screening models. Furthermore, the ability to express genes without the continuous use of selection markers opens up new possibilities for studying the pathobiology of this pathogen and advancing the development of novel therapeutic strategies against Acanthamoeba infections.

2,270 View
295 Download

Population genetic analysis of Plasmodium vivax vir genes in Pakistan: Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo; Parasites Hosts Dis 2024;62(3):313-322.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24036

Plasmodium vivax variant interspersed repeats (vir) refer to the key protein used for escaping the host immune system. Knowledge in the genetic variation of vir genes can be used for the development of vaccines or diagnostic methods. Therefore, we evaluated the genetic diversity of the vir genes of P. vivax populations of several Asian countries, including Pakistan, which is a malaria-endemic country experiencing a significant rise in malaria cases in recent years. We analyzed the genetic diversity and population structure of 4 vir genes (vir 4, vir 12, vir 21, and vir 27) in the Pakistan P. vivax population and compared these features to those of the corresponding vir genes in other Asian countries. In Pakistan, vir 4 (S=198, H=9, Hd=0.889, Tajima’s D value=1.12321) was the most genetically heterogenous, while the features of vir 21 (S=8, H=7, Hd=0.664, Tajima’s D value =-0.63763) and vir 27 (S =25, H =11, Hd =0.682, Tajima’s D value=-2.10836) were relatively conserved. Additionally, vir 4 was the most genetically diverse among Asian P. vivax populations, although within population diversity was low. Meanwhile, vir 21 and vir 27 among all Asian populations were closely related genetically. Our findings on the genetic diversity of vir genes and its relationships between populations in diverse geographical locations contribute toward a better understanding of the genetic characteristics of vir. The high level of genetic diversity of vir 4 suggests that this gene can be a useful genetic marker for understanding the P. vivax population structure. Longitudinal genetic diversity studies of vir genes in P. vivax isolates obtained from more diverse geographical areas are needed to better understand the function of vir genes and their use for the development of malaria control measures, such as vaccines.

Citations

Citations to this article as recorded by

Genetic polymorphisms of merozoite surface protein-3α in Plasmodium vivax isolates from Pakistan
Kim Oanh Nguyễn, Jung-Mi Kang, Tuấn Cường Võ, Hương Giang Lê, Seemab Akhtar, Thu Hằng Nguyễn, Đăng Thùy Dương Nguyễn, Minkyoung Cho, Sahib Gul Afridi, Byoung-Kuk Na
Acta Tropica.2025; 272: 107904. CrossRef
Genetic polymorphism of Duffy binding protein in Pakistan Plasmodium vivax isolates
Đăng Thùy Dương Nguyễn, Tuấn Cường Võ, Kim Oanh Nguyễn, Hương Giang Lê, Jung-Mi Kang, Thu Hằng Nguyễn, Minkyoung Cho, Sahib Gul Afridi, Byoung-Kuk Na
Acta Tropica.2024; 260: 107421. CrossRef

3,085 View
78 Download
2 Web of Science
Crossref

Prevalence and molecular analysis of glucose-6-phosphate dehydrogenase deficiency in Chin State, Myanmar: Ja Moon Aung, Zin Moon, Dorene VanBik, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zau Ring, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo; Parasites Hosts Dis 2023;61(2):154-162.
Published online May 23, 2023; DOI: https://doi.org/10.3347/PHD.23004

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is caused by X-linked recessive disorderliness. It induces severe anemia when a patient with G6PD deficiency is exposed to oxidative stress that occurs with administration of an antimalarial drug, primaquine. The distribution of G6PD deficiency remains unknown while primaquine has been used for malaria treatment in Myanmar. This study aimed to investigate the prevalence of G6PD deficiency and its variants in Chin State, Myanmar. Among 322 participants, 18 (11 males and 7 females) demonstrated a G6PD deficiency. Orissa variant was dominant in the molecular analysis. This would be related to neighboring Indian and Bangladeshi population, in which Orissa variant was also reported as the main mutation type. The screening test for G6PD deficiency before primaquine treatment appears to be important in Myanmar.

Citations

Citations to this article as recorded by

Glucose-6-phosphate dehydrogenase variants in Kachin, Myanmar
Zin Moon, Ja Moon Aung, Dorene VanBik, Hae Soo Yun, Sanghyun Lee, Sylvatrie-Danne Dinzouna-Boutamba, Zau Ring, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo
Parasites, Hosts and Diseases.2025; 63(4): 360. CrossRef
Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates: Correspondence
Amnuay Kleebayoon, Viroj Wiwanitkit
Parasites, Hosts and Diseases.2023; 61(3): 338. CrossRef

4,312 View
176 Download
2 Web of Science
Crossref

Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates from central Myanmar: Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zin Moon, Dong-Il Chung, Yeonchul Hong, Moe Kyaw Myint, Haung Naw, Byoung-Kuk Na, Youn-Kyoung Goo; Parasites Hosts Dis 2023;61(1):24-32.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22165

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the polymorphic var multigene family, is a highly polymorphic antigen that plays a crucial role in the pathology of malaria. The contribution of the genetic diversity of var toward the immune escape of P. falciparum has not yet been fully elucidated. This study aimed to characterize the diversity of var repertoires by screening P. falciparum Duffy-binding-like α domain (PfDBLα) among field isolates from central Myanmar. Genetic analysis revealed that the D-H segments of var in Myanmar populations have an extensive polymorphic repertoire, with high numbers of unique sequence types in each individual. However, var genes from the global population, including Myanmar, shared close genetic lineages regardless of their geographic origins, indicating that they have not undergone rapid evolutionary changes.

Citations

Citations to this article as recorded by

Exploring the Potential of miRNA-92a-3p as Lead for Sequence-Based Therapies for Malaria
Sowmya R. Prabhu, Sayandrila Paul, Shashikiran Umakanth, Manjunath Hande, Abdul Vahab Saadi, Himanshu Gupta, Kapaettu Satyamoorthy
Acta Parasitologica.2025;[Epub] CrossRef
Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates: Correspondence
Amnuay Kleebayoon, Viroj Wiwanitkit
Parasites, Hosts and Diseases.2023; 61(3): 338. CrossRef

5,300 View
183 Download
3 Web of Science
Crossref

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein: So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2022;60(1):1-6.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.1

Abstract
PDF

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein—Acanthamoeba silent-information regulator 2-like protein (AcSir2)—was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 μM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 μM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

Citations

Citations to this article as recorded by

Pterostilbene: A natural neuroprotective stilbene with anti-Alzheimer's disease properties
Songlan Gao, Honglei Zhang, Na Li, Lijuan Zhang, Zhe Zhu, Changlu Xu
Journal of Pharmaceutical Analysis.2025; 15(4): 101043. CrossRef
Alzheimer’s Disease: A Review of Molecular Mechanisms and Therapeutic Implications by Targeting Sirtuins, Caspases, and GSK-3
Kalpana Pandya, Krishnashish Roul, Avanish Tripathi, Sateesh Belemkar, Anshuman Sinha, Meryem Erol, Devendra Kumar
ACS Chemical Neuroscience.2025; 16(12): 2178. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells
Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha
Acta Tropica.2024; 257: 107288. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef

4,716 View
252 Download
5 Web of Science
Crossref

Case Reports

A Retrieved Sparganum of Spirometra erinaceieuropaei from a Korean Man during Mechanical Thrombectomy: Yang-Ha Hwang, Wonsoo Son, Yong-Won Kim, Dong-Hun Kang, Hyun-Ha Chang, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung; Korean J Parasitol 2020;58(3):309-313.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.309

Abstract
PDF

Human sparganosis is a zoonotic disease caused by infection and migration of the plerocercoid of Spirometra spp. Although sparganosis were reported from most parts of the body, the sparganum parasitizing inside cerebral artery is remarkably uncommon. We report a case of cerebral intravascular sparganosis in an elderly patient with acute ischemic stroke who was diagnosed by retrieving sparganum during mechanical thrombectomy. Finally, the parasites were identified as Spirometra erinaceieuropaei using multiplex PCR and cox1 gene sequencing.

Citations

Citations to this article as recorded by

Neglected zoonotic helminthiases in wild canids: new insights from South America
Manuel Uribe, Jan Brabec, Jenny J. Chaparro-Gutiérrez, Carlos Hermosilla
Frontiers in Veterinary Science.2023;[Epub] CrossRef

5,921 View
105 Download
1 Web of Science
Crossref

Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea: Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2019;57(4):417-422.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.417

Abstract
PDF

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

Citations

Citations to this article as recorded by

Exploring bioactive molecules released during inter- and intraspecific competition: A paradigm for novel antiparasitic drug discovery and design for human use
Pichet Ruenchit
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100256. CrossRef
Taeniasis impacts human gut microbiome composition and function
Wenjie Mu, Pingping Ma, Yugui Wang, Yaqi Li, Yingying Ding, Yang Zou, Lixia Pu, Qi Yan, Haoyue Kong, Xiaola Guo, Aijiang Guo, Hailong Li, Shuai Wang
The ISME Journal.2024;[Epub] CrossRef
Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China
Xiu-Min Han, Xue-Yong Zhang, Ying-Na Jian, Qing-Shan Tian
The Korean Journal of Parasitology.2021; 59(3): 311. CrossRef

11,888 View
239 Download
4 Web of Science
Crossref

Original Article

Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii: Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, H??ng Giang L?, Th? Lam Th?i, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2018;56(5):409-418.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.409

Abstract
PDF

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its expression was observed in both trophozoites and cysts. AcCP was also identified in cultured medium of A. castellanii. Considering to lysosomal localization, secretion or release by trophozoites and continuous expression in trophozoites and cysts, the enzyme could be a multifunctional enzyme that plays important biological functions for nutrition, development and pathogenicity of A. castellanii. These results also imply that AcCP can be a promising target for development of chemotherapeutic drug for Acanthamoeba infections.

Citations

Citations to this article as recorded by

Acanthamoeba castellanii cysteine protease 3 promotes M1 macrophage polarization through the TLR4/NF‑κB pathway
Zhi-xin Wang, Wan-jun Jiao, Mian-jing Wang, Yong Yang, Hai-long Wang, Hong-li Liu
Parasites & Vectors.2025;[Epub] CrossRef
Unraveling the interplay between unicellular parasites and bacterial biofilms: Implications for disease persistence and antibiotic resistance
Eva Zanditenas, Serge Ankri
Virulence.2024;[Epub] CrossRef
Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
Pathogens.2024; 13(2): 142. CrossRef
Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model
Rubén L. Rodríguez-Expósito, Ines Sifaoui, Lizbeth Salazar-Villatoro, Carlos J. Bethencourt-Estrella, José J. Fernández, Ana R. Díaz-Marrero, Robert Sutak, Maritza Omaña-Molina, José E. Piñero, Jacob Lorenzo-Morales
Marine Drugs.2024; 22(9): 423. CrossRef
The gene expression and proteomic profiling of Acanthamoeba isolates
Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta
Experimental Parasitology.2023; 255: 108630. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Induction of Programmed Cell Death in Acanthamoeba culbertsoni by the Repurposed Compound Nitroxoline
Rubén L. Rodríguez-Expósito, Ines Sifaoui, María Reyes-Batlle, Frieder Fuchs, Patrick L. Scheid, José E. Piñero, Robert Sutak, Jacob Lorenzo-Morales
Antioxidants.2023; 12(12): 2081. CrossRef
Paradigms of Protist/Bacteria Symbioses Affecting Human Health: Acanthamoeba species and Trichomonas vaginalis
Fiona L. Henriquez, Ronnie Mooney, Timothy Bandel, Elisa Giammarini, Mohammed Zeroual, Pier Luigi Fiori, Valentina Margarita, Paola Rappelli, Daniele Dessì
Frontiers in Microbiology.2021;[Epub] CrossRef
Trogocytosis in Unicellular Eukaryotes
Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
Cells.2021; 10(11): 2975. CrossRef
Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics
Issam Hasni, Philippe Decloquement, Sandrine Demanèche, Rayane Mouh Mameri, Olivier Abbe, Philippe Colson, Bernard La Scola
Microorganisms.2020; 8(5): 771. CrossRef
Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng
Parasites & Vectors.2020;[Epub] CrossRef
Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins
Abigail Betanzos, Cecilia Bañuelos, Esther Orozco
Genes.2019; 10(8): 618. CrossRef

8,359 View
168 Download
13 Web of Science
Crossref

Case Report

A Case of Furuncular Myiasis Due to Cordylobia anthropophaga in a Korean Traveler Returning from Uganda: Su-Min Song, Shin-Woo Kim, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2017;55(3):327-331.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.327

Abstract
PDF

A fly larva was recovered from a boil-like lesion on the left leg of a 33-year-old male on 21 November 2016. He has worked in an endemic area of myiasis, Uganda, for 8 months and returned to Korea on 11 November 2016. The larva was identified as Cordylobia anthropophaga by morphological features, including the body shape, size, anterior end, posterior spiracles, and pattern of spines on the body. Subsequent 28S rRNA gene sequencing showed 99.9% similarity (916/917 bp) with the partial 28S rRNA gene of C. anthropophaga. This is the first imported case of furuncular myiasis caused by C. anthropophaga in a Korean overseas traveler.

Citations

Citations to this article as recorded by

Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Origins and Diversification of Myiasis Across Blowflies
Gisele Antoniazzi Cardoso, Vanessa A. S. Cunha, Bruno C. Genevcius, Tais Madeira‐Ott, Bárbara Maria de Andrade Costa, Daniela Munhoz Rossoni, Patricia Jacqueline Thyssen, Tatiana Teixeira Torres
Ecology and Evolution.2025;[Epub] CrossRef
Incidence of Furuncular Myiasis due to Cordylobia anthropophaga (Blanchard, 1872) Larvae Infestation and Associated Risk Factors on Dogs in Ilorin Metropolis, North Central, Nigeria
Musa Rabiu, Ridwan Usman Adekola, Shola David Ola-Fadunsin, Isau Aremu Ganiyu, Miftah Olalekan Adeleke, Idiat Modupe Sanda, Ghali-Mohammed Ibraheem, Isyaku Abdulmajeed
UMYU Scientifica.2025; 4(1): 408. CrossRef
Human myiasis in Sub-Saharan Africa: A systematic review
Binta J. J. Jallow, Goudja Gassara, Ousman Bajinka, Yifei Luo, Mandie Liu, Jifeng Cai, Jingjing Huang, Fanming Meng, Nigel Beebe
PLOS Neglected Tropical Diseases.2024; 18(3): e0012027. CrossRef
Scrotal Myiasis in a Child Due to Cordylobia anthropophaga
Majed H Wakid, Yasser S Sharafeldein, Angham A Almakki, Dhuha A Alidrisi, Abeer A Bashinim
Cureus.2024;[Epub] CrossRef
Use of Commercial Bacon to Extract Maggots from an Infested Chronic Wound
Gregory Fenati, Santana Youssoffi, Shriya Vejendla, Cameron C. Neeki, Vinh T. Nguyen, Fanglong Dong, Michael M. Neeki
Advances in Skin & Wound Care.2024; 37(10): 555. CrossRef
Cutaneous myiasis by Calliphoridae dipterans in dogs from Chad
Rafael A.N. Ramos, Hassan Hakimi, Sidouin K. Metinou, Wachellet Danzabe, Macon Overcast, Jeremiah Cox, Rebecca Garabed, Philip Tchindebet Ouakou, Richard Ngandolo Bongo Nare, Fernando Torres-Velez, Lucienne Tritten, Meriam N. Saleh, Guilherme G. Verocai
Acta Tropica.2024; 260: 107454. CrossRef
Cordylobia anthropophaga Myiasis Mimicking Hyperproliferative Skin Disorder in Traveler Returning from Sub-Saharan Africa
Lidija Popović Dragonjić, Andrija Jović, Irena Janković, Jelena Miladinović, Aleksandar Ranković, Maja Cvetanović, Relja Beck, Dinko Novosel, Thomas Pape, Pavle Banović
Tropical Medicine and Infectious Disease.2023; 8(11): 505. CrossRef
Elephantoloemus indicus Austen, 1930 (Diptera: Calliphoridae) as the cause of cutaneous myiasis in captive Indian elephants from Assam, India
Prabhat Chandra Sarmah, Saidul Islam, Dilip Kumar Deka, Kanta Bhattacharjee, Kuntola Roy
Veterinary Parasitology: Regional Studies and Reports.2022; 32: 100734. CrossRef
Clogmia albipunctata (Nematocera; Psychodidae) as the Etiologic Agent of Myiasis: True or False?
Mohammad Akhoundi, Nambininiavo Marianne Ranorohasimanana, Sophie Brun, Catherine Kauffmann-Lacroix, Arezki Izri
Diagnostics.2022; 12(9): 2129. CrossRef
CRANIAL OSTEOMYELITIS AS A COMPLICATION OF FURUNCULAR MYIASIS
Nelson Muñoz, Sandra Galvis, Oscar Patiño, Carlos Moneriz
Revista Paulista de Pediatria.2021;[Epub] CrossRef
Human myiasis cases originating and reported in africa for the last two decades (1998–2018): A review
Simon K. Kuria, Adebola O. Oyedeji
Acta Tropica.2020; 210: 105590. CrossRef
Furuncular myiasis for the Western dermatologist: treatment in outpatient consultation
Romain Blaizot, Christophe Vanhecke, Philippe Le Gall, Alexandre Duvignaud, Marie‐Catherine Receveur, Denis Malvy
International Journal of Dermatology.2018; 57(2): 227. CrossRef
First case of Furuncular Myiasis due to Cordylobia anthropophaga in a Latin American resident returning from Central African Republic
Jóse A. Suárez, Argentina Ying, Luis A. Orillac, Israel Cedeño, Néstor Sosa
The Brazilian Journal of Infectious Diseases.2018; 22(1): 70. CrossRef
A Case of Cutaneous Myiasis Caused by Cordylobia anthropophaga Larvae in a Korean Traveler Returning from Central Africa
Joo Yeon Ko, In-Yong Lee, Byeong Jin Park, Jae Min Shin, Jae-Sook Ryu
The Korean Journal of Parasitology.2018; 56(2): 199. CrossRef

13,969 View
161 Download
17 Web of Science
Crossref

Original Article

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation: Ui-han Son, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Hae Soo Yun, Jung-Yeon Kim, So-Young Joo, Sookwan Jeong, Man Hee Rhee, Yeonchul Hong, Dong-Il Chung, Dongmi Kwak, Youn-Kyoung Goo; Korean J Parasitol 2017;55(2):149-158.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.149

Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (π and Θ_w), and Tajima’s D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima’s D values for vir 27 (1.08530, p>0.1), vir 12 (2.89007, p<0.01), and vir 21 (0.40782, p>0.1) were positive, and that of vir 4 (-1.32162, p>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.

Citations

Citations to this article as recorded by

Genetic diversity and natural selection analysis of VAR2CSA and vir genes: implication for vaccine development
Joseph Hawadak, Aditi Arya, Shewta Chaudhry, Vineeta Singh
Genomics & Informatics.2024;[Epub] CrossRef
Population genetic analysis of Plasmodium vivax vir genes in Pakistan
Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo
Parasites, Hosts and Diseases.2024; 62(3): 313. CrossRef
Immunological characterization of a VIR protein family member (VIR-14) in Plasmodium vivax-infected subjects from different epidemiological regions in Africa and South America
Raianna F. Fantin, Camila H. Coelho, Anne D. Berhe, Luisa M. D. Magalhães, Dhélio B. Pereira, Nichole D. Salinas, Niraj H. Tolia, Chanaki Amaratunga, Seila Suon, Issaka Sagara, David L. Narum, Ricardo T. Fujiwara, Claudia Abejon, Antonio Campos-Neto, Patr
PLOS Neglected Tropical Diseases.2023; 17(4): e0011229. CrossRef
Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
Youn-Kyoung Goo
Microorganisms.2022; 10(6): 1083. CrossRef
Genetic polymorphism of vir genes of Plasmodium vivax in Myanmar
Byoung-Kuk Na, Tong-Soo Kim, Khin Lin, Moon-Chang Baek, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo
Parasitology International.2021; 80: 102233. CrossRef
Humoral and cellular immune response to Plasmodium vivax VIR recombinant and synthetic antigens in individuals naturally exposed to P. vivax in the Republic of Korea
Sanghyun Lee, Young-Ki Choi, Youn-Kyoung Goo
Malaria Journal.2021;[Epub] CrossRef
Succinate dehydrogenase gene as a marker for studying Blastocystis genetic diversity
Adriana Higuera, Marina Muñoz, Myriam Consuelo López, Patricia Reyes, Plutarco Urbano, Oswaldo Villalobos, Juan David Ramírez
Heliyon.2020; 6(11): e05387. CrossRef
A bite to fight: front-line innate immune defenses against malaria parasites
Stephanie Tannous, Esther Ghanem
Pathogens and Global Health.2018; 112(1): 1. CrossRef
Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea
Young Yil Bahk, Jeonga Kim, Seong Kyu Ahn, Byoung-Kuk Na, Jong-Yil Chai, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(6): 545. CrossRef

11,996 View
155 Download
9 Web of Science
Crossref

Brief Communication

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(3):329-334.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.329

Abstract
PDF

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

Citations

Citations to this article as recorded by

Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson
Microbial Biotechnology.2025;[Epub] CrossRef
A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis
Zhenke Yang, Jinghui Wang, Yiming Qi, Yiping Shi, Fakun Li, Weijuan Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang
Parasites & Vectors.2024;[Epub] CrossRef
A novel fluoro colorimetric Loop mediated isothermal amplification (LAMP) assay for detection of Trichomonas vaginalis
Shoorashetty Manohar Rudresh, Pareyam Pooja, Pattacheravanda Nanaiah Shakuntala, Kanta Madhu
Indian Journal of Medical Microbiology.2024; 49: 100610. CrossRef
Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen
Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang
Acta Tropica.2024; 260: 107489. CrossRef
Label-free electrochemical DNA biosensing of MR TV 29 18s ribosomal RNA gene of Trichomonas vaginalis by signalization of non-spherical gold nanoparticles
R. Dehdari Vais, H. Heli, N. Sattarahmady
Materials Today Communications.2023; 34: 105123. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
A novel and ultrasensitive label-free electrochemical DNA biosensor for Trichomonas vaginalis detection based on a nanostructured film of poly(ortho-aminophenol)
Rezvan Dehdari Vais, Hossein Heli, Naghmeh Sattarahmady, Afshin Barazesh
Synthetic Metals.2022; 287: 117082. CrossRef
Omics Analyses of Trichomonas vaginalis Actin and Tubulin and Their Participation in Intercellular Interactions and Cytokinesis
Sebastián Lorenzo-Benito, Luis Alberto Rivera-Rivas, Lizbeth Sánchez-Ayala, Jaime Ortega-López, Octavio Montes-Flores, Daniel Talamás-Lara, Rossana Arroyo
Genes.2022; 13(6): 1067. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA
S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady
Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Loop mediated isothermal amplification assay for detection of Trichomonas vaginalis in vaginal swabs among symptomatic women from North India
S. Khurana, R. Dadwal, N. Sharma, A. Mewara, S. Singh, R. Bagga, R. Yadav, S. Sethi
Letters in Applied Microbiology.2020; 70(3): 196. CrossRef
Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Junjun Zhai, Zhang Yan, Feng Ping, Qu Lei, Xuelong Chen, Yanping Qi, Tianwen Wang
PLOS ONE.2020; 15(4): e0230881. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Label-free ultrasensitive electrochemical genosensing of Trichomonas vaginalis using anisotropic-shaped gold nanoparticles as a platform, a repeated sequence of the parasite DNA as a probe, and toluidine blue as a redox marker
N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. Heli
Sensors and Actuators B: Chemical.2018; 273: 234. CrossRef
Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
Shao-Xin Cai, Fan-De Kong, Shu-Fei Xu, Cui-Luan Yao
PeerJ.2018; 6: e5993. CrossRef

10,603 View
196 Download
15 Web of Science
Crossref

Original Article

Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2016;54(2):133-138.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.133

Abstract
PDF

Encystation is an essential process for Acanthamoeba survival under nutrient-limiting conditions and exposure to drugs. The expression of several genes has been observed to increase or decrease during encystation. Epigenetic processes involved in regulation of gene expression have been shown to play a role in several pathogenic parasites. In the present study, we identified the protein arginine methyltransferase 5 (PRMT5), a known epigenetic regulator, in Acanthamoeba castellanii. PRMT5 of A. castellanii (AcPRMT5) contained domains found in S-adenosylmethionine-dependent methyltransferases and in PRMT5 arginine-N-methyltransferase. Expression levels of AcPRMT5 were increased during encystation of A. castellanii. The EGFP-PRMT5 fusion protein was mainly localized in the nucleus of trophozoites. A. castellanii transfected with siRNA designed against AcPRMT5 failed to form mature cysts. The findings of this study lead to a better understanding of epigenetic mechanisms behind the regulation of encystation in cyst-forming pathogenic protozoa.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease
Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui
Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Free-living amoebae and squatters in the wild: ecological and molecular features
Ascel Samba-Louaka, Vincent Delafont, Marie-Hélène Rodier, Estelle Cateau, Yann Héchard
FEMS Microbiology Reviews.2019; 43(4): 415. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii
Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2017; 55(2): 109. CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef

10,214 View
103 Download
10 Web of Science
Crossref

Brief Communication

Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(1):75-80.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.75

Abstract
PDF

This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR detected T. vaginalis in 12 (1.9%) patients. Testing via PCR demonstrated high sensitivity and high negative predictive value for T. vaginalis. Among the 19 women who tested positive for T. vaginalis according to PCR, 94.7% (18/19) reported vaginal signs and symptoms. Notably, more than 50% of T. vaginalis infections occurred in females younger than 30 years old, and 58% were unmarried. Multiplex PCR, which simultaneously detects pathogens from various sexually transmitted infections, revealed that 91.7% (11/12) of patients were infected with 2 or more pathogens. Mycoplasma hominis was the most prevalent co-infection pathogen with T. vaginalis, followed by Ureaplasma urealyticum and Chlamydia trachomatis. Our results indicate that PCR and multiplex PCR are the most sensitive tools for T. vaginalis diagnosis, rather than microscopy which has been routinely used to detect T. vaginalis infections in South Korea. Therefore, clinicians should take note of the high prevalence of T. vaginalis infections among adolescent and young women in order to prevent persistent infection and transmission of this disease.

Citations

Citations to this article as recorded by

Diagnostic accuracy of real-time polymerase chain reaction assay for the detection of Trichomonas vaginalis in clinical samples: A systematic review and meta-analysis
Emmanuel O. Babafemi, Benny P. Cherian, Khalid Rahman, Gilbert M. Mogoko, Oluwatoyin O. Abiola
African Journal of Laboratory Medicine.2025;[Epub] CrossRef
The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis
Zeinab Moghadamizad, Javad Yazdizadeh Khalili, Meysam Olfatifar, Milad Badri, Sasan Khazaei
International Health.2024; 16(3): 240. CrossRef
Combination of genitourinary candidiasis and sexually transmitted infections
N.E. Portnyagina, A.K. Kvardakova, V.V. Pakhomova, E.G. Gubanova, N.V. Deeva, I.G. Sergeeva
Russian Journal of Clinical Dermatology and Venereology.2024; 23(4): 377. CrossRef
Trichomonas vaginalis: comparison of primers for implementation as an in-house PCR in rural Vellore, South India
Nagarajan L. Surya, Thangamani Suji, Santhosh Rani, Irene Dorathy, Shantidani Minz, Rani Diana Sahni
BMC Infectious Diseases.2024;[Epub] CrossRef
Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Comparison of Diagnostic Methods for Detection of Trichomonas vaginalis in Prediagnosed Vaginitis Cases and Its Association with Various Pathogens
Vildan Turan Faraşat, İbrahim Cüneyt Balcıoğlu, Pınar Solmaz Hasdemir, Ertaç Gümüş
Turkish Journal of Parasitology.2022; 46(3): 167. CrossRef
Trichomonas vaginalis follow-up and persistence in Colombian women
Lauren Hernández-Buelvas, Milena Camargo, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
Scientific Reports.2021;[Epub] CrossRef
Trichomoniasis in a tertiary hospital of Madrid, Spain (2013–2017): prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis
Celia Bolumburu, Vega Zamora, María Muñoz-Algarra, Francisca Portero-Azorín, José Antonio Escario, Alexandra Ibáñez-Escribano
Parasitology Research.2020; 119(6): 1915. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
PREVALENCE OF TRICHOMONIASIS IN ASYMPTOMATIC PREGNANT WOMEN POPULATION IN BANDUNG, WEST JAVA, INDONESIA
Pati Aji Achdiat, Reiva Farah Dwiyana, Vina Feriza, Rasmia Rowawi, Rendy Ariezal Effendi, Oki Suwarsa, Hendra Gunawan
Indonesian Journal of Tropical and Infectious Disease.2019; 7(4): 57. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(3): 329. CrossRef
Epidemiology of Trichomoniasis in South Korea and Increasing Trend in Incidence, Health Insurance Review and Assessment 2009-2014
So-Young Joo, Youn-Kyoung Goo, Jae-Sook Ryu, Sang-Eun Lee, Won Kee Lee, Dong-Il Chung, Yeonchul Hong, Zhefeng Meng
PLOS ONE.2016; 11(12): e0167938. CrossRef

11,073 View
150 Download
11 Web of Science
Crossref

Case Reports

Comorbid Gastric Adenocarcinoma and Gastric and Duodenal Strongyloides stercoralis Infection: A Case Report: An Na Seo, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong, Ohkyoung Kwon, Han-Ik Bae; Korean J Parasitol 2015;53(1):95-99.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.95

Abstract
PDF

Strongyloides stercoralis can cause systemic infection, termed strongyloidiasis, and gastrointestinal ulcer disease in immunocompromised patients. However, to our knowledge, there are no reported cases of comorbid gastric adenocarcinoma and S. stercoralis infection. Here, we report a case of an 81-year-old Korean man who presented with S. stercoralis infection coexisting with early gastric adenocarcinoma (T1aN0M0). S. stercoralis eggs, rhabditiform larvae, and adult females were observed in normal gastric and duodenal crypts. They were also observed in atypical glands representative of adenocarcinoma and adenoma. Preliminary laboratory tests revealed mild neutrophilic and eosinophilic leukocytosis. A routine stool test failed to detect rhabditiform larvae in the patient’s fecal sample; however, S. stercoralis was identified by PCR amplification and 18S rRNA sequencing using genomic DNA extracted from formalin-fixed paraffin-embedded tissues. Postoperatively, the patient had a persistent fever and was treated with albendazole for 7 days, which alleviated the fever. The patient was followed-up by monitoring and laboratory testing for 4 months postoperatively, and no abnormalities were observed thus far. The fact that S. stercoralis infection may be fatal in immunocompromised patients should be kept in mind when assessing high-risk patients.

Citations

Citations to this article as recorded by

Helminth-induced immune modulation in colorectal cancer: exploring therapeutic applications
Hongyu Li, Chaojun Shan, Yunhuan Zhu, Xiaodong Yao, Lijun Lin, Xiaofen Zhang, Yuncheng Qian, Yuqing Wang, Jialu Xu, Yijie Zhang, Hairun Li, Ling Zhao, Keda Chen
Frontiers in Immunology.2025;[Epub] CrossRef
Relationship between parasites and lung cancer: Unveiling the link
Mariam T. El Khadrawe, Nahla El Skhawy, Maha M. Eissa
Tropical Medicine & International Health.2025; 30(7): 613. CrossRef
NEOPLASIA AND PROLIFERATIVE LESIONS IN FREE-RANGING MOUNTAIN AND GRAUER'S GORILLAS (GORILLA BERINGEI)
Maya L. Iyer, Kirsten V. Gilardi, Michael R. Cranfield, Sarah M. Corner, Eddy Kambale Syaluha, Jean Bosco Noheri, Benard Ssebide, Jean Felix Kinani, Fred Nizeyimana, Ricky Okwir Okello, Julius Nziza, Martin Kabuyaya, Methode Bahizi, Richard Muvunyi, Elisa
Journal of Zoo and Wildlife Medicine.2025;[Epub] CrossRef
Gastroduodenal Strongyloidiasis Diagnosed during Iron Deficiency Anemia Workup
Malek Kreidieh, Neville Mobarakai, Sherif Andrawes, Robert Colef, Lara Kreidieh, Hassan Al Moussawi, Jean M. Chalhoub
Case Reports in Gastroenterology.2025; 19(1): 467. CrossRef
The Parasitism and Tumors Carcinogenesis: A Review Subject
Ahmed Salih Alshewered
Acta Parasitologica.2024; 69(1): 183. CrossRef
A case of disseminated strongyloidiasis diagnosed by worms in the urinary sediment
Young-Ha Lee
Parasites, Hosts and Diseases.2024; 62(2): 238. CrossRef
Mining parasites for their potential as novel therapeutic agents against cancer
Neha Sylvia Walter, Shalmoli Bhattacharyya
Medical Oncology.2024;[Epub] CrossRef
Association between Some Helminths and Tumorigenesis through Immunological and Biochemical Factors
Maryam Fasihi-Karami, Reza Afrisham, Saina Karami, Ehsan Beigzadeh, Forough Kazemi
Current Cancer Therapy Reviews.2023; 19(2): 96. CrossRef
STRONGYLOIDES STERCORALIS: A RARE CASE DIAGNOSED WITH ESOPHAGEAL SWAB SAMPLE
Arzu Hazal AYDIN, Buse BAYAZIT GÖZÜKÜÇÜK, Nesrin TURHAN
Kırıkkale Üniversitesi Tıp Fakültesi Dergisi.2023; 25(1): 166. CrossRef
Is Gastric Involvement by Strongyloides stercoralis in an Immunocompetent Patient a Common Finding? A Case Report and Review of the Literature
Irene Pecorella, Tom Richard Okello, Gaia Ciardi, David Martin Ogwang
Acta Parasitologica.2022; 67(1): 94. CrossRef
Seropositivity Rates of Strongyloides stercoralis Antibody in the Southeastern Region of Republic of Korea: A Single-Center Retrospective Study
Taehwa Kim, Seungjin Lim
The Korean Journal of Parasitology.2022; 60(3): 181. CrossRef
Strongyloides Hyperinfection Causing Gastrointestinal Bleeding and Bacteremia in an Immunocompromised Patient
Juan Carlos De la Cruz Mayhua, Bisharah Rizvi
Cureus.2021;[Epub] CrossRef
Bacterial and Parasitic Pathogens as Risk Factors for Cancers in the Gastrointestinal Tract: A Review of Current Epidemiological Knowledge
Janneke W. Duijster, Eelco Franz, Jacques Neefjes, Lapo Mughini-Gras
Frontiers in Microbiology.2021;[Epub] CrossRef
Colorectal Cancer Related to Chronic Strongyloides stercoralis Infection
M. Sava, T. Huynh, A. Frugoli, L. Kong, M. Salehpour, B. Barrows
Case Reports in Gastrointestinal Medicine.2020; 2020: 1. CrossRef
Phylogenetic Positioning of a Strongyloides stercoralis Isolate Recovered from a Korean Patient and Comparison with Other Asian Isolates
Jaeho Bae, Mi Jin Jeong, Dong hoon Shin, Hyun Woo Kim, Sung Ho Ahn, Jun Ho Choi, Hak Sun Yu
The Korean Journal of Parasitology.2020; 58(6): 689. CrossRef
Microbiome signatures in prostate cancer
Sagarika Banerjee, James C Alwine, Zhi Wei, Tian Tian, Natalie Shih, Colin Sperling, Thomas Guzzo, Michael D Feldman, Erle S Robertson
Carcinogenesis.2019; 40(6): 749. CrossRef
Pathologic Features of Infectious Gastritis
Eric U. Yee, Enoch Kuo, Jeffrey D. Goldsmith
Advances in Anatomic Pathology.2018; 25(4): 238. CrossRef
The Clean India Mission: Public and animal health benefits
Rashmi Thakur, Balbir Bagicha Singh, Prateek Jindal, Rabinder Singh Aulakh, Jatinder Paul Singh Gill
Acta Tropica.2018; 186: 5. CrossRef
Compensatory gastric stretching following subtotal gastric resection due to gastric adenocarcinoma in a diamond python (Morelia spilota spilota)
HR Baron, J Šlapeta, SL Donahoe, RTJ Doneley, DN Phalen
Australian Veterinary Journal.2018; 96(12): 481. CrossRef
Parasite Infection, Carcinogenesis and Human Malignancy
Hoang van Tong, Paul J. Brindley, Christian G. Meyer, Thirumalaisamy P. Velavan
EBioMedicine.2017; 15: 12. CrossRef
Gastric Strongyloidiasis: Report of 2 Cases and Brief Review of the Literature
Rawia Mohamed, Mowafak M. Hamodat, Mousa A. Al-Abbadi
Laboratory Medicine.2017; 48(1): 93. CrossRef
Strongyloidiasis Current Status with Emphasis in Diagnosis and Drug Research
Tiago Mendes, Karen Minori, Marlene Ueta, Danilo Ciccone Miguel, Silmara Marques Allegretti
Journal of Parasitology Research.2017; 2017: 1. CrossRef
A case of adenocarcinoma developed in the small intestine with chronic strongyloidiasis
Satoshi Ishikawa, Tamaki Maeda, Kimiaki Hattori, Takahiro Watanabe, Takanori Kuramoto, Saori Ueno, Goro Ueno, Tadahiro Yamada, Akifumi Kanazawa, Masahiro Sakaguchi, Yoshitane Tsukamoto
Clinical Journal of Gastroenterology.2017; 10(6): 519. CrossRef
Colorectal Cancer Associated with Strongyloides stercoralis Colitis
Carmine Catalano, Joshua Aron, Raghav Bansal, Anatoly Leytin
ACG Case Reports Journal.2017; 4(1): e104. CrossRef
Carcinogenesis associated with parasites other than Schistosoma, Opisthorchis and Clonorchis: A systematic review
Claudia Machicado, Luis A. Marcos
International Journal of Cancer.2016; 138(12): 2915. CrossRef

16,996 View
160 Download
24 Web of Science
Crossref

Extracorporeal Worm Extraction of Diphyllobothrium nihonkaiense with Amidotrizoic Acid in a Child: Hye Kyung Shin, Joo-Hyung Roh, Jae-Won Oh, Jae-Sook Ryu, Youn-Kyoung Goo, Dong-Il Chung, Yong Joo Kim; Korean J Parasitol 2014;52(6):677-680.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.677

Abstract
PDF

Infection cases of diphyllobothriid tapeworms are not much in the below teen-age group. We report a case of Diphyllobothrium nihonkaiense infection in a 13-year-old boy. He presented with severe fatigue, occasional abdominal pain at night time. He also had several episodes of tapeworm segment discharge in his stools. By his past history, he had frequently eaten raw fish including salmon and trout with his families. Numerous eggs of diphyllobothriid tapeworm were detected in the fecal examination. We introduced amidotrizoic acid as a cathartic agent through nasogastroduodenal tube and let nearly whole length (4.75 m) of D. nihonkaiense be excreted through his anus. After a single dose of praziquantel, the child's stool showed no further eggs, and his symptoms disappeared. The evacuated worm was identified as D. nihonkaiense by mitochondrial cox1 gene analysis. Here we report a successful extracorporeal worm extraction from an infection case of D. nihonkaiense by the injection of amidotrizoic acid.

Citations

Citations to this article as recorded by

Diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from Korea
Seoyun Choi, Jaeeun Cho, Bong-Kwang Jung, Deok-Gyu Kim, Sarah Jiyoun Jeon, Hyeong-Kyu Jeon, Keeseon S. Eom, Jong-Yil Chai
Parasitology Research.2015; 114(6): 2129. CrossRef

17,084 View
115 Download
2 Web of Science
Crossref

Brief Communication

Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea: Jun-Hyeok Seo, Hye-Won Yang, So-Young Joo, Su-Min Song, Yu-Ran Lee, Jae-Sook Ryu, Eun Sang Yoo, Won Kee Lee, Hyun-Hee Kong, Sang-Eun Lee, Won-Ja Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(5):551-555.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.551

Abstract
PDF

Trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. Despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including PCR, few prospective studies present data on male T. vaginalis infection in South Korea. In the present study, the prevalence of T. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary care urology clinic in South Korea. The prevalence of T. vaginalis infection in our cohort was 4% (8/201) by PCR. T. vaginalis infection was common in men older than 40 years (median age, 52 years). Among the 8 Trichomonas-positive patients, 87.5% (7/8) had prostatic diseases, such as prostatitis and benign prostatic hyperplasia, and 25.0% (2/8) and 12.5% (1/8) were coinfected with Chlamydia trachomatis and Mycoplasma genitalium, respectively. Our results suggest that T. vaginalis infection is not rare in men attending primary care urology clinics in South Korea, especially in those older than 40 years, in whom it may explain the presence of prostatic disease. The possibility of T. vaginalis infection should be routinely considered in older male patients with prostatic diseases in South Korea.

Citations

Citations to this article as recorded by

Trichomoniasis in Men: A Neglected Factor in Male Infertility?
Seyed Ali Hosseini, Mohammad Matini, Maryam Bahmanzadeh, Reza Aslani, Faeze Foroughi-Parvar
Acta Parasitologica.2025;[Epub] CrossRef
Systematic review and meta-analysis of the global prevalence and infection risk factors of Trichomonas vaginalis
Wenjie Tian, Yuhua Li, Yani Zhang, Yiming Zhang, Yiran Qin, Yalin Han, Dongxian Li, Shuai Wang, Zhenke Yang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang
Parasite.2025; 32: 56. CrossRef
Prevalence of trichomoniasis infection among adults in Nigerian community settings
K. Chukwuemeka Obetta, Innocent Okonkwo Ogbonna, Dorida Nneka Oyigbo, Oliver Onyemaechi Ugwu, Kenneth Okonkwo Ugwu, Beatrice N. Onah, Chinasa Maryrose Ugwunnadi, Joseph O. Acha, Ngozi Uzoamaka Chuke, Ogechi Nkemjika, Onyinyechi Elizabeth Okoye
Medicine.2023; 102(37): e34585. CrossRef
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China
Zhenchao Zhang, Yuhui Sang, Pucheng Wu, Yujia Shang, Lesong Li, Yujuan Duan, Linfei Zhao, Minghui Gao, Lihua Guo, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lixia Hao, Xuefang Mei, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Urogenital Trichomonas vaginalis infection in males: a case report and retrospective analysis of a 10‐year period in a tertiary hospital
Miguel Alpalhão, Luís Marques‐Lito, Paulo Filipe, João Borges‐Costa
International Journal of Dermatology.2022;[Epub] CrossRef
Seroprevalence and risk factors of Trichomonas vaginalis among couples in Al-Hamza city-Iraq.
Musafer H. Al-Ardi
Al-Kufa University Journal for Biology.2021; 13(1): 26. CrossRef
Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men
Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park
The Korean Journal of Parasitology.2019; 57(1): 21. CrossRef
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2019; 57(1): 27. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(1): 75. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef

11,534 View
127 Download
13 Web of Science
Crossref

Mini Review

New Molecules in Babesia gibsoni and Their Application for Diagnosis, Vaccine Development, and Drug Discovery: Youn-Kyoung Goo, Xuenan Xuan; Korean J Parasitol 2014;52(4):345-353.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.345

Abstract
PDF

Babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. B. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. Since no vaccine is available, rapid and accurate diagnosis and prompt treatment of infected animals are required to control this disease. Over the past decade, several candidate molecules have been identified using biomolecular techniques in the authors' laboratory for the development of a serodiagnostic method, vaccine, and drug for B. gibsoni. This review article describes newly identified candidate molecules and their applications for diagnosis, vaccine production, and drug development of B. gibsoni.

Citations

Citations to this article as recorded by

Standardization of quantitative PCR (qPCR) method to detect the level of parasitaemia in Babesia gibsoni infected dogs
Varuna Purushothama Panicker, Athira Narayanan, Ajith Kumar Sreedharan Nair, Anjaly Krishnan, Nimna Ajay, Vinod Kumar
Journal of Microbiological Methods.2024; 224: 107009. CrossRef
Phylogenetic analysis of Babesia gibsoni isolates of south India using apical membrane antigen, 50 kDa surface antigen, and 70 kDa heat shock protein genes
Chundayil Kalarickal Deepa, Anju Varghese, Christophe Angeline Felicia Bora, Karapparambu Gopalan Ajith Kumar, Lijo John, Muhasin Asaf, Sunanda Chulliparambil, Reghu Ravindran
Experimental Parasitology.2023; 246: 108461. CrossRef
Identification of three members of the multidomain adhesion CCp family in Babesia gibsoni
Hang Li, Shengwei Ji, Eloiza May Galon, Iqra Zafar, Zhuowei Ma, Thom Do, Moaz M. Amer, Yihong Ma, Junya Yamagishi, Mingming Liu, Xuenan Xuan
Acta Tropica.2023; 241: 106890. CrossRef
Babesia gibsoni Whole-Genome Sequencing, Assembling, Annotation, and Comparative Analysis
Qin Liu, Xing-Ai Guan, Dong-Fang Li, Ya-Xin Zheng, Sen Wang, Xue-Nan Xuan, Jun-Long Zhao, Lan He, Jian Li
Microbiology Spectrum.2023;[Epub] CrossRef
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
Chundayil Kalarickal Deepa, Anju Varghese, Karapparambu Gopalan Ajith Kumar, Ashwathappa Nandini, Gatchanda Shravan Kumar, Prabodh Kumar Hembram, Chemmangattuvalappil Narendranath Dinesh, Sanis Juliet, Jess Vergis, Ollukkara Krishnan Sindhu, Reghu Ravindr
Experimental Parasitology.2023; 254: 108621. CrossRef
Babesia gibsoni
Mingming Liu, Ikuo Igarashi, Xuenan Xuan
Trends in Parasitology.2022; 38(9): 815. CrossRef
Molecular survey and phylogenetic analysis of Babesia vogeli in dogs
Abdelfattah Selim, Ameer Megahed, Mourad Ben Said, Abdullah D. Alanazi, Mohamed Z. Sayed-Ahmed
Scientific Reports.2022;[Epub] CrossRef
Establishment and Application of an Indirect Enzyme-Linked Immunosorbent Assay for Measuring GPI-Anchored Protein 52 (P52) Antibodies in Babesia gibsoni-Infected Dogs
Qin Liu, Xueyan Zhan, Dongfang Li, Junlong Zhao, Haiyong Wei, Heba Alzan, Lan He
Animals.2022; 12(9): 1197. CrossRef
Evaluation of the Inhibitory Effects of Six Natural Product Extracts against Babesia gibsoni in Vitro and in Vivo
Xiaohu Zhai, Lingxu Li, Peihao Zhang, Yiwen Guo, Huaide Jiang, Weihua He, Yanyan Li, Bin Zhang, Dawei Yao
Journal of Parasitology.2022;[Epub] CrossRef
In vitro screening of novel anti-Babesia gibsoni drugs from natural products
Shengwei Ji, Mingming Liu, Eloiza May Galon, Mohamed Abdo Rizk, Jixu Li, Yongchang Li, Iqra Zafar, Ikuo Igarashi, Xuenan Xuan
Parasitology International.2021; 85: 102437. CrossRef
The Infection and Species Identification of Canine Babesia spp. in Parts of Shaanxi Province
Wuren Ma, Huan Tang, Yu Zhou, Guanghui Zhao, Yunpeng Fan, Xiaoping Song, Junke Song
Journal of Parasitology.2021;[Epub] CrossRef
Closing the empty anti-Babesia gibsoni drug pipeline in vitro using fluorescence-based high throughput screening assay
Mohamed Abdo Rizk, Shengwei Ji, Mingming Liu, Shimaa Abd El-Salam El-Sayed, Yongchang Li, Benedicto Byamukama, Aaron Edmond Ringo, Xuenan Xuan, Ikuo Igarashi
Parasitology International.2020; 75: 102054. CrossRef
Babesia canis spp. in dogs in Baghdad Province, Iraq: First molecular identification and clinical and epidemiological study
Naseir Mohammed Badawi, Afaf Abdulrahman Yousif
Veterinary World.2020; 13(3): 579. CrossRef
Survey and Molecular Study of Babesia gibsoni in Dogs of Baghdad Province, Iraq
Naseir M. Badawi, Afaf A. Yousif
The Iraqi Journal of Veterinary Medicine.2020; 44((E0)): 34. CrossRef
To kill a piroplasm: genetic technologies to advance drug discovery and target identification in Babesia
Caroline D. Keroack, Brendan Elsworth, Manoj T. Duraisingh
International Journal for Parasitology.2019; 49(2): 153. CrossRef
Babesia gibsoni endemic to Wuhan, China: mitochondrial genome sequencing, annotation, and comparison with apicomplexan parasites
Jiaying Guo, Xiaoyan Miao, Pei He, Muxiao Li, Sen Wang, Jie Cui, Cuiqin Huang, Lan He, Junlong Zhao
Parasitology Research.2019; 118(1): 235. CrossRef
Inhibitory effects of the phytohormone inhibitors fluridone and inabenfide against Babesia gibsoni in vitro
Mingming Liu, Tatsunori Masatani, Paul Franck Adjou Moumouni, Seung-Hun Lee, Eloiza May Galon, Yang Gao, Huanping Guo, Jixu Li, Yongchang Li, Xuenan Xuan
Veterinary Parasitology.2019; 265: 19. CrossRef
Evaluation of Babesia gibsoni GPI-anchored Protein 47 (BgGPI47-WH) as a Potential Diagnostic Antigen by Enzyme-Linked Immunosorbent Assay
Xueyan Zhan, Long Yu, Xiaomeng An, Qin Liu, Muxiao Li, Zheng Nie, Yangnan Zhao, Sen Wang, Yangsiqi Ao, Yu Tian, Lan He, Junlong Zhao
Frontiers in Veterinary Science.2019;[Epub] CrossRef
Identification and characterization of interchangeable cross-species functional promoters between Babesia gibsoni and Babesia bovis
Mingming Liu, Paul Franck Adjou Moumouni, Shinuo Cao, Masahito Asada, Guanbo Wang, Yang Gao, Huanping Guo, Jixu Li, Patrick Vudriko, Artemis Efstratiou, Aaron Edmond Ringo, Seung-Hun Lee, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga, Shin-ichiro Kawaz
Ticks and Tick-borne Diseases.2018; 9(2): 330. CrossRef
Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Hassan Hakimi, Tatsunori Masatani, Patrick Vudriko, Seung-Hun Lee, Shin-ichiro Kawazu, Junya Yamagishi, Xuenan Xuan
Parasites & Vectors.2018;[Epub] CrossRef
Two Imported Cases of Babesiosis with Complication or Co-Infection with Lyme Disease in Republic of Korea
Hea Yoon Kwon, Jae Hyoung Im, Yun-Kyu Park, Areum Durey, Jin-Soo Lee, Ji Hyeon Baek
The Korean Journal of Parasitology.2018; 56(6): 609. CrossRef
Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter
Mingming Liu, Masahito Asada, Shinuo Cao, Paul Franck Adjou Moumouni, Patrick Vudriko, Artemis Efstratiou, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga, Shin-ichiro Kawazu, Junya Yamagishi, Xuenan Xuan
Molecular and Biochemical Parasitology.2017; 216: 56. CrossRef

15,278 View
143 Download
23 Web of Science
Crossref

Brief Communication

Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea): Su-Min Song, Dinzouna-Boutamba Sylvatrie-Danne, So-Young Joo, Yun Kyung Shin, Hak Sun Yu, Yong-Seok Lee, Ji-Eon Jung, Noboru Inoue, Won Kee Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(3):305-310.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.305

Abstract
PDF

Ascidian soft tunic syndrome (AsSTS) caused by Azumiobodo hoyamushi (A. hoyamushi) is a serious aquaculture problem that results in mass mortality of ascidians. Accordingly, the early and accurate detection of A. hoyamushi would contribute substantially to disease management and prevention of transmission. Recently, the loop-mediated isothermal amplification (LAMP) method was adopted for clinical diagnosis of a range of infectious diseases. Here, the authors describe a rapid and efficient LAMP-based method targeting the 18S rDNA gene for detection of A. hoyamushi using ascidian DNA for the diagnosis of AsSTS. A. hoyamushi LAMP assay amplified the DNA of 0.01 parasites per reaction and detected A. hoyamushi in 10 ng of ascidian DNA. To validate A. hoyamushi 18S rDNA LAMP assays, AsSTS-suspected and non-diseased ascidians were examined by microscopy, PCR, and by using the LAMP assay. When PCR was used as a gold standard, the LAMP assay showed good agreement in terms of sensitivity, positive predictive value (PPV), and negative predictive value (NPV). In the present study, a LAMP assay based on directly heat-treated samples was found to be as efficient as DNA extraction using a commercial kit for detecting A. hoyamushi. Taken together, this study shows the devised A. hoyamushi LAMP assay could be used to diagnose AsSTS in a straightforward, sensitive, and specific manner, that it could be used for forecasting, surveillance, and quarantine of AsSTS.

Citations

Citations to this article as recorded by

Measurement of Tunic Hardness in an Edible Ascidian, Halocynthia roretzi, with Remarks on Soft Tunic Syndrome
Euichi Hirose, Kei Nakayama, Tetsuya Yanagida, Akatsuki Nawata, Shin-Ichi Kitamura
Zoological Science.2018; 35(6): 548. CrossRef

9,446 View
92 Download
2 Web of Science
Crossref

Case Report

Two Human Cases of Diphyllobothrium nihonkaiense Infection in Korea: Su-Min Song, Hye-Won Yang, Min Kyu Jung, Jun Heo, Chang Min Cho, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung; Korean J Parasitol 2014;52(2):197-199.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.197

Abstract
PDF

Diphyllobothrium latum and Diphyllobothrium nihonkaiense are the 2 reported main causes of human diphyllobothriasis in the Republic of Korea. However, the differentiation of these 2 species based on morphologic features alone is difficult. The authors used nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene to diagnose Diphyllobothrium spp. Two patients visited the emergency room at Kyungpook National University Hospital on 3 April and 12 April 2013, respectively, with fragments of parasites found while defecating. The parasites were identified as Diphyllobothrium spp. based on morphologic characteristics, and subsequent cox1 gene sequencing showed 99.9% similarity (1,478/1,480 bp) with D. nihonkaiense. Our findings support the hypothesis that D. nihonkaiense is a dominant species in Korea.

Citations

Citations to this article as recorded by

Diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from Korea
Seoyun Choi, Jaeeun Cho, Bong-Kwang Jung, Deok-Gyu Kim, Sarah Jiyoun Jeon, Hyeong-Kyu Jeon, Keeseon S. Eom, Jong-Yil Chai
Parasitology Research.2015; 114(6): 2129. CrossRef
Three Cases of Diphyllobothrium nihonkaiense Infection in Korea
Hong-Ja Kim, Keeseon S. Eom, Min Seo
The Korean Journal of Parasitology.2014; 52(6): 673. CrossRef
Extracorporeal Worm Extraction of Diphyllobothrium nihonkaiense with Amidotrizoic Acid in a Child
Hye Kyung Shin, Joo-Hyung Roh, Jae-Won Oh, Jae-Sook Ryu, Youn-Kyoung Goo, Dong-Il Chung, Yong Joo Kim
The Korean Journal of Parasitology.2014; 52(6): 677. CrossRef

9,053 View
100 Download
6 Web of Science
Crossref

Original Articles

Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2014;52(2):131-135.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.131

Abstract
PDF

Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii
Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio
Nature Protocols.2024; 19(1): 3. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Suthinee Sangkanu, Abolghasem Siyadatpanah, Roghayeh Norouzi, Julalak Chuprom, Watcharapong Mitsuwan, Sirirat Surinkaew, Rachasak Boonhok, Alok K. Paul, Tooba Mahboob, Imran Sama-ae, Sonia M. R. Oliveira, Tajudeen O. Jimoh, Maria de Lourdes Pereira, Polra
PeerJ.2024; 12: e16937. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Evolution of giant pandoravirus revealed by CRISPR/Cas9
Hugo Bisio, Matthieu Legendre, Claire Giry, Nadege Philippe, Jean-Marie Alempic, Sandra Jeudy, Chantal Abergel
Nature Communications.2023;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens
Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Wilawan Mahabusarakam, L. Ravithej Singh, Ekachai Dumkliang, Kritamorn Jitrangsri, Alok K. Paul, Sirirat Surinkaew, Polrat Wilairatana, Maria de Lourdes Pereira, Mohammed Rahmatu
PeerJ.2022; 10: e14468. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage
Ruixuan Zhang, Hisashi Endo, Masaharu Takemura, Hiroyuki Ogata, Samuel K. Campos
Microbiology Spectrum.2021;[Epub] CrossRef
Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector
Christopher Price, Snake Jones, Mirna Mihelcic, Marina Santic, Yousef Abu Kwaik
Cell Host & Microbe.2020; 27(4): 571. CrossRef
Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene in Acanthamoeba castellanii
Steven Rolland, Luce Mengue, Cyril Noël, Stéphanie Crapart, Anne Mercier, Willy Aucher, Yann Héchard, Ascel Samba-Louaka
Pathogens.2020; 9(5): 321. CrossRef
The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
Parasites & Vectors.2020;[Epub] CrossRef
Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture
Mária Garajová, Martin Mrva, Naděžda Vaškovicová, Michal Martinka, Janka Melicherová, Andrea Valigurová
Scientific Reports.2019;[Epub] CrossRef
The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls
Pamela Magistrado-Coxen, Yousuf Aqeel, Angelo Lopez, John R. Haserick, Breeanna R. Urbanowicz, Catherine E. Costello, John Samuelson, Photini Sinnis
PLOS Neglected Tropical Diseases.2019; 13(5): e0007352. CrossRef
A review of Acanthamoeba keratitis in the middle East and Iran
MohammadHossein Feiz Haddad, Azar Shokri, Habib Habibpour, SeyedeManizhe Heidar Neiadi
Journal of Acute Disease.2019; 8(4): 133. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii
Duo Wu, Meng Feng, Zhi-xin Wang, Ke Qiao, Hiroshi Tachibana, Xun-jia Cheng
Parasites & Vectors.2018;[Epub] CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef
In-vitro development of an effective treatment for Acanthamoeba keratitis
Á. Ortillés, J. Belloc, E. Rubio, M.T. Fernández, M. Benito, J.Á. Cristóbal, B. Calvo, P. Goñi
International Journal of Antimicrobial Agents.2017; 50(3): 325. CrossRef
Treatment of Acanthamoeba keratitis
N. R. Marchenko, Evg. A. Kasparova
Vestnik oftal'mologii.2016; 132(5): 110. CrossRef
Cellulose degradation: a therapeutic strategy in the improved treatment of Acanthamoeba infections
Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Parasites & Vectors.2015;[Epub] CrossRef
An update onAcanthamoebakeratitis: diagnosis, pathogenesis and treatment
Jacob Lorenzo-Morales, Naveed A. Khan, Julia Walochnik
Parasite.2015; 22: 10. CrossRef
Potential Value of Cellulose Synthesis Inhibitors Combined With PHMB in the Treatment of Acanthamoeba Keratitis
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Cornea.2015; 34(12): 1593. CrossRef

11,302 View
127 Download
24 Web of Science
Crossref

Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba: Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2013;51(3):269-277.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.269

Abstract
PDF

Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.

Citations

Citations to this article as recorded by

Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii
Susanna Haapanen, Maarit S. Patrikainen, Seppo Parkkila
Diagnostic Microbiology and Infectious Disease.2023; 107(2): 116014. CrossRef
A simple and visible detection method for the rapid diagnosis of Ustilaginoidea virens in rice seeds by a loop‐mediated isothermal amplification assay
Wei Wang, Hang Yin, Ning Huang, Cuijing Zhu, Yufei Wang, Xintong Qi, Lu Ma, Yunxin Fan, Yao Yu, Hongsheng Zhang, Yongmei Bao
Journal of Phytopathology.2021; 169(6): 369. CrossRef
Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample
Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
Scientific Reports.2021;[Epub] CrossRef
Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis of Plasmodium vivax Malaria in a Tertiary Hospital in Chandigarh, North India
Hargobinder Kaur, Rakesh Sehgal, Devendra Bansal, Ali A. Sultan, Ashish Bhalla, Sunit C. Singhi
The American Journal of Tropical Medicine and Hygiene.2018; 98(5): 1374. CrossRef
Detection of Acanthamoeba spp. in water samples collected from natural water reservoirs, sewages, and pharmaceutical factory drains using LAMP and PCR in China
Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
Science of The Total Environment.2017; 584-585: 489. CrossRef
Water-borne protozoa parasites: The Latin American perspective
Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
International Journal of Hygiene and Environmental Health.2017; 220(5): 783. CrossRef
Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
Indian Journal of Medical Microbiology.2017; 35(1): 90. CrossRef
Acanthamoeba keratitis: improving the Scottish diagnostic service for the rapid molecular detection of Acanthamoeba species
Claire Low Alexander, Michael Coyne, Brian Jones, Deepa Anijeet
Journal of Medical Microbiology .2015; 64(7): 682. CrossRef
Molecular diagnosis in clinical parasitology: When and why?
Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
Experimental Biology and Medicine.2014; 239(11): 1443. CrossRef