Parasites, Hosts and Diseases

"apoptosis"

Original Article

Toxoplasma gondii IST suppresses inflammatory and apoptotic responses by inhibiting STAT1-mediated signaling in IFN-γ/TNF-α-stimulated hepatocytes: Seung-Hwan Seo, Ji-Eun Lee, Do-Won Ham, Eun-Hee Shin; Parasites Hosts Dis 2024;62(1):30-41.
Published online February 23, 2024; DOI: https://doi.org/10.3347/PHD.23129

The dense granule protein of Toxoplasma gondii, inhibitor of signal transducer and activator of transcription 1 (IST) is an inhibitor of signal transducer and activator of transcription 1 (STAT1) transcriptional activity that binds to STAT1 and regulates the expression of inflammatory molecules in host cells. A sterile inflammatory liver injury in pathological acute liver failures occurs when excessive innate immune function, such as the massive release of IFN-γ and TNF-α, is activated without infection. In relation to inflammatory liver injury, we hypothesized that Toxoplasma gondii inhibitor of STAT1 transcription (TgIST) can inhibit the inflammatory response induced by activating the STAT1/IRF-1 mechanism in liver inflammation. This study used IFN-γ and TNF-α as inflammatory inducers at the cellular level of murine hepatocytes (Hepa-1c1c7) to determine whether TgIST inhibits the STAT1/IRF-1 axis. In stable cells transfected with TgIST, STAT1 expression decreased with a decrease in interferon regulatory factor (IRF)-1 levels. Furthermore, STAT1 inhibition of TgIST resulted in lower levels of NF-κB and COX2, as well as significantly lower levels of class II transactivator (CIITA), iNOS, and chemokines (CLXCL9/10/11). TgIST also significantly reduced the expression of hepatocyte proapoptotic markers (Caspase3/8/9, P53, and BAX), which are linked to sterile inflammatory liver injury. TgIST also reduced the expression of adhesion (ICAM-1 and VCAM-1) and infiltration markers of programmed death-ligand 1 (PD-L1) induced by hepatocyte and tissue damage. TgIST restored the cell apoptosis induced by IFN-γ/TNF-α stimulation. These results suggest that TgIST can inhibit STAT1-mediated inflammatory and apoptotic responses in hepatocytes stimulated with proinflammatory cytokines.

Citations

Citations to this article as recorded by

Toxoplasma GRA16 attenuates Tau hyperphosphorylation and enhances autophagy in thrombin-treated HT-22 hippocampal neuronal cells
Seung-Hwan Seo, Do-Won Ham, Ji-Eun Lee, Eun-Hee Shin
Scientific Reports.2025;[Epub] CrossRef
Toxoplasma gondii GRA16 Suppresses Aerobic Glycolysis by Downregulating c-Myc and TERT Expressions in Colorectal Cancer Cells
Ji-Eun Lee, Seung-Hwan Seo, Do-Won Ham, Eun-Hee Shin
Biomolecules & Therapeutics.2025; 33(4): 621. CrossRef

6,855 View
535 Download
2 Web of Science
Crossref

Brief Communication

Excretory-Secretory Products of Trichomonas vaginalis Cause Apoptosis in Mouse Sperm in Vitro: Jihyun Keum, Jaesook Roh, Jae-Sook Ryu, Ki-Young Ryu; Korean J Parasitol 2022;60(5):357-360.
Published online October 21, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.5.357

Abstract
PDF

Excretory-secretory products (ESP) of T. vaginalis have been shown to inhibit sperm motility, viability, and functional integrity, leading to a decreased fertilization rate in vitro. This study investigated whether T. vaginalis induce apoptosis and ultrastructural changes of sperm using flow cytometry and electron microscopy. Incubation of sperm with T. vaginalis ESP increased phosphatidylserine externalization and DNA fragmentation, and decreased mitochondrial membrane potential. Transmission electron microscopy of sperm incubated with ESP revealed abnormal features such as distorted heads, broken necks, and acrosomes exocytosis. This is the first report that demonstrates a direct impact of T. vaginalis ESP on sperm apoptosis and architecture in vitro.

Citations

Citations to this article as recorded by

The possible pathogenic mechanisms of microorganisms in infertility: a narrative review
Zahra Chegini, Amin Khoshbayan, Milad Kashi, Raha Zare Shahraki, Mojtaba Didehdar, Aref Shariati
Archives of Microbiology.2025;[Epub] CrossRef

3,623 View
122 Download
2 Web of Science
Crossref

Original Articles

Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line: Hao Wang, Chunchao Li, Wei Ye, Zhaobin Pan, Jinhui Sun, Mingzhu Deng, Weiqiang Zhan, Jiaqi Chu; Korean J Parasitol 2021;59(6):573-583.
Published online December 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.6.573

Toxoplasma gondii, an intracellular protozoan parasite that infects one-third of the world’s population, has been reported to hijack host cell apoptotic machinery and promote either an anti- or proapoptotic program depending on the parasite virulence and load and the host cell type. However, little is known about the regulation of human FHs 74 small intestinal epithelial cell viability in response to T. gondii infection. Here we show that T. gondii RH strain tachyzoite infection or ESP treatment of FHs 74 Int cells induced apoptosis, mitochondrial dysfunction and ER stress in host cells. Pretreatment with 4-PBA inhibited the expression or activation of key molecules involved in ER stress. In addition, both T. gondii and ESP challenge-induced mitochondrial dysfunction and cell death were dramatically suppressed in 4-PBA pretreated cells. Our study indicates that T. gondii infection induced ER stress in FHs 74 Int cells, which induced mitochondrial dysfunction followed by apoptosis. This may constitute a potential molecular mechanism responsible for the foodborne parasitic disease caused by T. gondii.

Citations

Citations to this article as recorded by

Bug as a drug: Unveiling anti-cancer properties of Toxoplasma gondii and its therapeutic prospects in cancer immunotherapy
Yie Wei Chua, Sek Chuen Chow
Acta Tropica.2025; 267: 107684. CrossRef
Apicomplexan Espionage: Orchestrated Miscommunication at the Host–Parasite Interface
Vaisak Mohan, Sandeep Reddy Vinjamuri, Parnika Sahoo, Abhinav Hatwar, Sandra S N, Usha Krishna, Vyshnav P V, Kamalakannan Vijayan
ACS Infectious Diseases.2024; 10(12): 4040. CrossRef
Evaluation of the inhibitory effects and the mechanism of terpenoids on Toxoplasma gondii tachyzoites
Zi-Yang Jiang, Kai Dong, Zheng-Song Huang, Chang-Chun Yuan, Bo Liu, Qi-Wei Chen, Jian-Ping Chen
Acta Tropica.2023; 237: 106741. CrossRef
Toxoplasma gondii induces MLTC-1 apoptosis via ERS pathway
Lei Wang, Hailun Wang, Shihao Wei, Xiaoyu Huang, Chunchen Yu, Qingrui Meng, Dengfeng Wang, Guangwen Yin, Zhijian Huang
Experimental Parasitology.2023; 244: 108429. CrossRef
Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350
Xiao-Jing Wu, Jin Gao, Bing-Jin Mu, Lin-Mei Yu, Zi-Rui Wang, Wen-Bin Zheng, Wen-Wei Gao, Xing-Quan Zhu, Qing Liu
Frontiers in Veterinary Science.2022;[Epub] CrossRef

5,320 View
145 Download
5 Web of Science
Crossref

Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis: Qi Xiao, Yuhong Hu, Xiaohong Yang, Jianna Tang, Xiaoshuang Wang, Xiaomin Xue, Mengxue Li, Minjing Wang, Yinan Zhao, Jingze Liu, Hui Wang; Korean J Parasitol 2020;58(2):161-171.
Published online April 30, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.2.161

Abstract
PDF

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

Citations

Citations to this article as recorded by

Transcriptome reveals the roles and potential mechanisms of CeRNA in the regulation of salivary gland development in the tick Rhipicephalus haemaphysaloides
Shanming Hu, Songqin Chen, Haotian Zhu, Yanan Wang, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Jinlin Zhou
Frontiers in Cellular and Infection Microbiology.2025;[Epub] CrossRef
Quantitative proteomic analysis of salivary glands in female Ornithodoros lahorensis (syn. Alveonasus lahorensis) after blood feeding via DIA-coupled LC-MS/MS
Xiangfen Yuan, Xiaolin Xu, Huiyu Wang, Hongli Jing, Songyin Qiu, Yufang Kong, Yue Zhang, Jizhou Lv, Xun Suo, Shaoqiang Wu
Ticks and Tick-borne Diseases.2025; 16(6): 102575. CrossRef
Protein regulation mechanism of cold tolerance in Haemaphysalis longicornis
Ningmei Wang, Aimeng Ji, Abolfazl Masoudi, Shuang Li, Yuhong Hu, Yefei Zhang, Zhijun Yu, Han Wang, Hui Wang, Jingze Liu
Insect Science.2023; 30(3): 725. CrossRef
Effect of chlorpyrifos on the expression and regulation of salivary gland protein of Haemaphysalis longicornis
Xiaomin Xue, Ningmei Wang, Jingyi Ma, Aimeng Ji, Han Wang, Mengxue Li, Xiaohan Di, Jingze Liu, Hui Wang
International Journal of Acarology.2023; 49(2): 154. CrossRef
Phosphoproteomic Analysis of Haemaphysalis longicornis Saliva Reveals the Influential Contributions of Phosphoproteins to Blood-Feeding Success
Desmond O. Agwunobi, Ningmei Wang, Lei Huang, Yefei Zhang, Guomin Chang, Kuang Wang, Mengxue Li, Hui Wang, Jingze Liu
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef

6,181 View
112 Download
4 Web of Science
Crossref

Brief Communication

Parasite Infiltration and Apoptosis in Spleen upon Toxoplasma gondii Infection: Su-Hwa Lee, Ki-Back Chu, Fu-Shi Quan; Korean J Parasitol 2019;57(5):537-541.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.537

Abstract
PDF

Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.

Citations

Citations to this article as recorded by

Ginsenoside Rb1/baicalein nanoparticles: a potential nanomedicine for toxoplasmosis therapy
Yingmei Lai, Zhongao Zhang, Xintian Yu, Zhuohang Cheng, Songrui Wu, Jianzu Ding, Wenkang Huang, Shaohong Lu, Huayue Ye, Haojie Ding, Xunhui Zhuo
Acta Tropica.2025; 269: 107740. CrossRef
Recombinant vaccinia virus expressing MIC8, AMA1, or RON4 induce protection against Toxoplasma gondii ME49 strain infection
Hae-Ji Kang, Fu-Shi Quan
Acta Tropica.2025; 270: 107812. CrossRef
Early immune response to Toxoplasma gondii lineage III isolates of different virulence phenotype
Aleksandra Uzelac, Ivana Klun, Olgica Djurković-Djaković
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Inflammatory response and parasite regulation in acute toxoplasmosis: the role of P2X7 receptor in controlling virulent atypical genotype strain of Toxoplasma gondii
Thuany Prado-Rangel, Aline Cristina Abreu Moreira-Souza, Sthefani Rodrigues Batista da Silva, Thais Barboza-Araujo, Archimedes Barbosa Castro-Junior, Isalira Peroba Rezende Ramos, Christina Maeda Takiya, Rossiane Claudia Vommaro, Robson Coutinho-Silva
Frontiers in Immunology.2024;[Epub] CrossRef
Toxoplasma gondii induces MST2 phosphorylation mediating the activation of hippo signaling pathway to promote apoptosis and lung tissue damage
Kangzhi Xu, Shifan Zhu, Fan Xu, Jin Yang, Bin Deng, Dingzeyang Su, Jing Ma, Mingyue Zu, Yifan Lin, Tianxu Pei, Yuyang Zhu, Lele Wang, Dandan Liu, Qiangde Duan, Jinjun Xu, Zhiming Pan, Jianping Tao, Zhaofeng Hou
iScience.2024; 27(12): 111312. CrossRef
Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues
Jéssica S. Souza, Priscila S. G. Farani, Beatriz I. S. Ferreira, Helene S. Barbosa, Rubem F. S. Menna-Barreto, Otacilio C. Moreira, Rafael M. Mariante
Frontiers in Microbiology.2023;[Epub] CrossRef
TNF-α Triggers RIP1/FADD/Caspase-8-Mediated Apoptosis of Astrocytes and RIP3/MLKL-Mediated Necroptosis of Neurons Induced by Angiostrongylus cantonensis Infection
Hongli Zhou, Minyu Zhou, Yue Hu, Yanin Limpanon, Yubin Ma, Ping Huang, Paron Dekumyoy, Wanchai Maleewong, Zhiyue Lv
Cellular and Molecular Neurobiology.2022; 42(6): 1841. CrossRef
The potential risks of herbicide butachlor to immunotoxicity via induction of autophagy and apoptosis in the spleen
Shanshan Zhu, Yingwei Liu, Yangwei Li, Jiangnan Yi, Bijing Yang, Yuanliang Li, Zhuanxu Ouyang, Bingxian Liu, Peng Shang, Khalid Mehmood, Rao Zahid Abbas, Shakeel Ahmed, Yung-Fu Chang, Jianying Guo, Jiaqiang Pan, Lianmei Hu, Zhaoxin Tang, Ying Li, Hui Zhan
Chemosphere.2022; 286: 131683. CrossRef
Synthesis, Characterization and Nanoformulation of Novel Sulfonamide-1,2,3-triazole Molecular Conjugates as Potent Antiparasitic Agents
Faizah S. Aljohani, Nadjet Rezki, Mohamed R. Aouad, Bassma H. Elwakil, Mohamed Hagar, Eman Sheta, Nermine Mogahed Fawzy Hussein Mogahed, Sanaa K. Bardaweel, Nancy Abd-elkader Hagras
International Journal of Molecular Sciences.2022; 23(8): 4241. CrossRef
Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis
Samah Hassan Yahia, Samia Elsayed Etewa, Nesreen Saeed Saleh, Samira Metwally Mohammad, Nora Ibrahim Aboulfotouh, Ahmad Mansour Kandil, Mohamed Hassan Sarhan, José F. Silveira
Journal of Parasitology Research.2022; 2022: 1. CrossRef

7,160 View
98 Download
11 Web of Science
Crossref

Original Articles

Neuronal Apoptosis: Pathological Basis of Behavioral Dysfunctions Induced by Angiostrongylus cantonensis in Rodents Model: Shiqi Luo, Lisi OuYang, Jie Wei, Feng Wu, Zhongdao Wu, Wanlong Lei, Dongjuan Yuan; Korean J Parasitol 2017;55(3):267-278.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.267

Angiostrongylus cantonensis invades the central nervous system (CNS) of humans to induce eosinophilic meningitis and meningoencephalitis and leads to persistent headache, cognitive dysfunction, and ataxic gait. Infected mice (nonpermissive host), admittedly, suffer more serious pathological injuries than rats (permissive host). However, the pathological basis of these manifestations is incompletely elucidated. In this study, the behavioral test, histological and immunohistochemical techniques, and analysis of apoptotic gene expression, especially caspase-3, were conducted. The movement and motor coordination were investigated at week 2 post infection (PI) and week 3 PI in mice and rats, respectively. The cognitive impairs could be found in mice at week 2 PI but not in rats. The plaque-like lesion, perivascular cuffing of inflammatory cells, and dilated vessels within the cerebral cortex and hippocampus were more serious in mice than in rats at week 3 PI. Transcriptomic analysis showed activated extrinsic apoptotic pathway through increased expression of TNFR1 and caspase-8 in mice CNS. Immunohistochemical and double-labeling for NeuN and caspase-3 indicated the dramatically increased expression of caspase-3 in neuron of the cerebral cortex and hippocampus in mice but not in rats. Furthermore, western-blotting results showed high expression of cleaved caspase-3 proteins in mice but relatively low expression in rats. Thus, extrinsic apoptotic pathway participated in neuronal apoptosis might be the pathological basis of distinct behavioral dysfunctions in rodents with A. cantonensis infection. It provides the evidences of a primary molecular mechanism for the behavioral dysfunction and paves the ways to clinical diagnosis and therapy for A. cantonensis infection.

Citations

Citations to this article as recorded by

Insights into the biology of the rat lungworm, Angiostrongylus cantonensis
Chasen D. Griffin, Vanessa O. Ezenwa, Robert H. Cowie
Parasites & Vectors.2025;[Epub] CrossRef
Defective glycolysis in the cerebrum and cerebellum correlates with the pathology and neurological declines in mice with Angiostrongylus cantonensis infection
Shih-Yi Peng, Ho Yin Pekkle Lam, Yu-Ting Huang
Parasitology International.2024; 98: 102821. CrossRef
Angiostrongylus cantonensis induces energy imbalance and dyskinesia in mice by reducing the expression of melanin-concentrating hormone
Hui Huang, Zhongyuan Zhang, Mengdan Xing, Zihan Jin, Yue Hu, Minyu Zhou, Hang Wei, Yiwen Liang, Zhiyue Lv
Parasites & Vectors.2024;[Epub] CrossRef
IL-17A Mediates Demyelination by Activating A1 Astrocytes via SOCS3 During Angiostrongylus cantonensis Infection
Zongpu Zhou, Tuo Lin, Zhen Liu, Qian Ding, Zhixuan Ma, Wanqi Li, Fukang Xie, Yue Lan, Ying Feng
Frontiers in Immunology.2022;[Epub] CrossRef
Mechanisms of the host immune response and helminth-induced pathology during Trichobilharzia regenti (Schistosomatidae) neuroinvasion in mice
Tomáš Macháček, Roman Leontovyč, Barbora Šmídová, Martin Majer, Oldřich Vondráček, Iveta Vojtěchová, Tomáš Petrásek, Petr Horák, Elise O’Connell
PLOS Pathogens.2022; 18(2): e1010302. CrossRef
Improvements of cognitive functions in mice heavily infected by Angiostrongylus cantonensis after treatment with albendazole, dexamethasone, or co-therapy
Kai-Yuan Jhan, Chien-Ju Cheng, Chih-Jen Chou, Shih-Ming Jung, Guan-Jhih Lai, Kuang-Yao Chen, Lian-Chen Wang
Journal of Microbiology, Immunology and Infection.2022; 55(5): 935. CrossRef
Necroptosis and Caspase-2-Mediated Apoptosis of Astrocytes and Neurons, but Not Microglia, of Rat Hippocampus and Parenchyma Caused by Angiostrongylus cantonensis Infection
Hongli Zhou, Zhe Chen, Yanin Limpanont, Yue Hu, Yubin Ma, Ping Huang, Paron Dekumyoy, Minyu Zhou, Yixin Cheng, Zhiyue Lv
Frontiers in Microbiology.2020;[Epub] CrossRef
Severe CNS angiostrongyliasis in a young marine: a case report and literature review
Liane McAuliffe, Shannon Fortin Ensign, Derek Larson, Mary Bavaro, Joseph Yetto, Michael Cathey, Mitsuru Mukaigawara, Masashi Narita, Kiyofumi Ohkusu, Timothy Quast, Charles Volk
The Lancet Infectious Diseases.2019; 19(4): e132. CrossRef
Regulatory effect of host miR-101b-3p on parasitism of nematode Angiostrongylus cantonensis via superoxide dismutase 3
Dongjuan Yuan, Shiqi Luo, Lian Xu, Xingda Zeng, Zhongdao Wu
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms.2019; 1862(5): 557. CrossRef
Trichostatin A, a Histone Deacetylase Inhibitor, Alleviates Eosinophilic Meningitis Induced by Angiostrongylus cantonensis Infection in Mice
Yanhua Zhang, Hui Xie, Wenyan Tang, Xingda Zeng, Yu Lin, Lian Xu, Lihua Xiao, Jun Xu, Zhongdao Wu, Dongjuan Yuan
Frontiers in Microbiology.2019;[Epub] CrossRef
MicroRNA-125b mimic inhibits ischemia reperfusion-induced neuroinflammation and aberrant p53 apoptotic signalling activation through targeting TP53INP1
Xiao-Qian Li, Qian Yu, Wen-Fei Tan, Zai-Li Zhang, Hong Ma
Brain, Behavior, and Immunity.2018; 74: 154. CrossRef
Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection
Zhang Mengying, Xu Yiyue, Pan Tong, Hu Yue, Yanin Limpanont, Huang Ping, Kamolnetr Okanurak, Wu Yanqi, Paron Dekumyoy, Zhou Hongli, Dorn Watthanakulpanich, Wu Zhongdao, Wang Zhi, Lv Zhiyue
Parasites & Vectors.2017;[Epub] CrossRef

10,315 View
151 Download
13 Web of Science
Crossref

Tamoxifen Induces Apoptosis of Leishmania major Promastigotes in Vitro: Masoud Doroodgar, Mahdi Delavari, Moein Doroodgar, Ali Abbasi, Ali Akbar Taherian, Abbas Doroodgar; Korean J Parasitol 2016;54(1):9-14.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.9

Abstract
PDF

Tamoxifen is an antagonist of the estrogen receptor and currently used for the treatment of breast cancer. The current treatment of cutaneous leishmaniasis with pentavalent antimony compounds is not satisfactory. Therefore, in this study, due to its antileishmanial activity, effects of tamoxifen on the growth of promastigotes and amastigotes of Leishmania major Iranian strain were evaluated in vitro. Promastigotes and amastigotes were treated with different concentrations (1, 5, 10, 20, and 50 μg/ml) and time periods (24, 48, and 72 hr) of tamoxifen. After tamoxifen treatment, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5 biphenyl tetrazolium bromide assay) was used to determine the percentage of live parasites and Graph Pad Prism software to calculate IC₅₀. Flow cytometry was applied to investigate the induction of tamoxifen-induced apoptosis in promastigotes. The half maximal inhibitory concentration (IC₅₀) of tamoxifen on promastigotes was 2.6 μg/ml after 24 hr treatment. Flow cytometry analysis showed that tamoxifen induced early and late apoptosis in Leishmania promastigotes. While after 48 hr in control group the apoptosis was 2.0%, the 50 ?g/L concentration of tamoxifen increased it to 59.7%. Based on the in vitro antileishmanial effect, tamoxifen might be used for leishmaniasis treatment; however, further researches on in vivo effects of tamoxifen in animal models are needed.

Citations

Citations to this article as recorded by

Therapeutic effect of topical tamoxifen in murine cutaneous leishmaniasis caused by Leishmania major
Parastoo Hassani-Abharian, Seyedamirmehdi Hejazi Dehaghani, Zabihollah Shahmoradi, Seyed Hossein Hejazi, Audrey Odom John
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Compared Antileishmanial Activity of Clomiphene and Tamoxifen
Sergio Sifontes-Rodríguez, Alma Reyna Escalona-Montaño, Ricardo Mondragón Flores, Niurka Mollineda-Diogo, Lianet Monzote Fidalgo, Mónica Edith Mondragón-Castelán, Fedra Alardin-Gutiérrez, Lourdes Araceli López-Enzana, Daniel Andrés Sánchez-Almaraz, Ofelia
Biomedicines.2024; 12(10): 2290. CrossRef
Caryocar coriaceumWittm. fruit extracts asLeishmaniainhibitors:in-vitroandin-silicoapproaches
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Selene Maia de Morais, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Taylon Felipe Silva, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Idessania Nazareth Costa, Emmanuel Silva Marinho, Mar
Journal of Biomolecular Structure and Dynamics.2022; 40(17): 8040. CrossRef
Antileishmanial Activity of Tamoxifen by Targeting Sphingolipid Metabolism: A Review
Kaleab Alemayehu Zewdie, Haftom Gebregergs Hailu, Muluken Altaye Ayza, Bekalu Amare Tesfaye
Clinical Pharmacology: Advances and Applications.2022; Volume 14: 11. CrossRef
The potential role and apoptotic profile of three medicinal plant extracts on Leishmania tropica by MTT assay, macrophage model and flow cytometry analysis
Mozhde Ilaghi, Iraj Sharifi, Fariba Sharififar, Fatemeh Sharifi, Razieh Tavakoli Oliaee, Zahra Babaei, Manzume Shamsi Meimamandi, Alireza Keyhani, Mehdi Bamorovat
Parasite Epidemiology and Control.2021; 12: e00201. CrossRef
Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
Jasem Saki, Khalil Saki, Reza Arjmand
Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub] CrossRef
Selective Estrogen Receptor Modulators Against Gram-Positive and Gram-Negative Bacteria: An Experimental Study
Aakriti Garg, Arti Singh, Anoop Kumar
Future Microbiology.2021; 16(13): 987. CrossRef
Network-Based Approaches Reveal Potential Therapeutic Targets for Host-Directed Antileishmanial Therapy Driving Drug Repurposing
J. Eduardo Martinez-Hernandez, Zaynab Hammoud, Alessandra Mara de Sousa, Frank Kramer, Rubens L. do Monte-Neto, Vinicius Maracaja-Coutinho, Alberto J. M. Martin, Tim Downing
Microbiology Spectrum.2021;[Epub] CrossRef
Male predominance in reported Visceral Leishmaniasis cases: Nature or nurture? A comparison of population-based with health facility-reported data
Kristien Cloots, Sakib Burza, Paritosh Malaviya, Epco Hasker, Sangeeta Kansal, Guy Mollett, Jaya Chakravarty, Nurpur Roy, Bibek Kumar Lal, Suman Rijal, Shyam Sundar, Marleen Boelaert, Guilherme L. Werneck
PLOS Neglected Tropical Diseases.2020; 14(1): e0007995. CrossRef
Selective Estrogen Receptor Modulators (SERMs): Mechanistic Insights Against Microbial Infections
Aakriti Garg, Balraj Singh, Ruchika Sharma, Arti Singh, Anoop Kumar
Current Molecular Medicine.2020; 20(2): 102. CrossRef
Epidemiology, Associated Factors and Treatment Methods of Cutaneous Leishmaniasis Based on Previous Data from 2013 to 2018 in Ilam, Western Iran
Nasrin Rezaee, Vahid Raissi, Ahmad Rajaeipour, Mehdi Nazari, Muhammad Getso, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim
Acta Parasitologica.2020; 65(3): 760. CrossRef
Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage
João Paulo Assolini, Fernanda Tomiotto-Pellissier, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Sergio Marques Borghi, Waldiceu Apareci
Journal of Drug Targeting.2020; 28(10): 1110. CrossRef
4-nitrochalcone exerts leishmanicidal effect on L. amazonensis promastigotes and intracellular amastigotes, and the 4-nitrochalcone encapsulation in beeswax copaiba oil nanoparticles reduces macrophages cytotoxicity
João Paulo Assolini, Thais Peron da Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Claudia Sayer, Pe
European Journal of Pharmacology.2020; 884: 173392. CrossRef
Repurposing azole antifungals into antileishmanials: Novel 3-triazolylflavanones with promising in vitro antileishmanial activity against Leishmania major
Masoud Keighobadi, Saeed Emami, Mahdi Fakhar, Azar Shokri, Hassan Mirzaei, Saeed Hosseini Teshnizi
Parasitology International.2019; 69: 103. CrossRef
Study of Ethinyl Estradiol Activity Against Promastigotes, Axenic and Macrophage-Dwelling Amastigotes of Leishmania infantum by Using Atomic Force Microscopy and Methyl Thiazolyl Tetrazolium Methods
Farnoush Shadnoush, Reza Arjmand, Fakher Rahim, Jasem Saki
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Repurposing as a strategy for the discovery of new anti-leishmanials: the-state-of-the-art
REBECCA L. CHARLTON, BARTIRA ROSSI-BERGMANN, PAUL W. DENNY, PATRICK G. STEEL
Parasitology.2018; 145(2): 219. CrossRef
Caryocar coriaceum extracts exert leishmanicidal effect acting in promastigote forms by apoptosis-like mechanism and intracellular amastigotes by Nrf2/HO-1/ferritin dependent response and iron depletion
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Milena Menegazzo Miranda-Sapla, Selene Maia de Morais, João Paulo Assolini, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Allan Henrique Depieri Cataneo, Danielle Kian, Tiago Bervelieri Ma
Biomedicine & Pharmacotherapy.2018; 98: 662. CrossRef
Epidemiological Aspects of Cutaneous Leishmaniasis during 2009-2016 in Kashan City, Central Iran
Doroodgar Moein, Doroodgar Masoud, Mahboobi Saeed, Doroodgar Abbas
The Korean Journal of Parasitology.2018; 56(1): 21. CrossRef
Tamoxifen Never Ceases to Amaze: New Findings on Non-Estrogen Receptor Molecular Targets and Mediated Effects
Tatiana Anatolievna Bogush, Boris Borisovich Polezhaev, Ivan Andreevich Mamichev, Elena Alexandrovna Bogush, Boris Evseevich Polotsky, Sergei Alexeevich Tjulandin, Andrey Borisovich Ryabov
Cancer Investigation.2018; 36(4): 211. CrossRef
Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy
Débora P. Aureliano, José Angelo Lauletta Lindoso, Sandra Regina de Castro Soares, Cleusa Fumika Hirata Takakura, Thiago Martini Pereira, Martha Simões Ribeiro
Photodiagnosis and Photodynamic Therapy.2018; 23: 1. CrossRef
Grandiflorenic acid promotes death of promastigotes via apoptosis-like mechanism and affects amastigotes by increasing total iron bound capacity
Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, João Paulo Assolini, Amanda Cristina Machado Carloto, Priscila Goes Camargo de Carvalho, Ian Lucas Alves Cardoso, Andréa Name Colado
Phytomedicine.2018; 46: 11. CrossRef
In vitro Effects of Ketotifen and Cromolyn Sodium on Promastigote and Amastigotes of Leishmania major
Lima Asgharpour Sarouey, Khadijeh Khanaliha, Parvaneh Rahimi-Moghaddam, Samaneh Khorrami, Mohammad Saaid Dayer, Fatemeh Tabatabaie
Jundishapur Journal of Microbiology.2018;[Epub] CrossRef
Repurposing Estrogen Receptor Antagonists for the Treatment of Infectious Disease
Marhiah C. Montoya, Damian J. Krysan, Danielle A. Garsin
mBio.2018;[Epub] CrossRef
Library of Seleno-Compounds as Novel Agents against Leishmania Species
Álvaro Martín-Montes, Daniel Plano, Rubén Martín-Escolano, Verónica Alcolea, Marta Díaz, Silvia Pérez-Silanes, Socorro Espuelas, Esther Moreno, Clotilde Marín, Ramón Gutiérrez-Sánchez, Carmen Sanmartín, Manuel Sánchez-Moreno
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef

10,858 View
165 Download
29 Web of Science
Crossref

Brief Communication

Quantitative Evaluation of Viability- and Apoptosis-Related Genes in Ascaris suum Eggs under Different Culture-Temperature Conditions: Yong-Man Yu, You-Hang Cho, Young-Nam Youn, Juan Hua Quan, In-Wook Choi, Young-Ha Lee; Korean J Parasitol 2012;50(3):243-247.
Published online August 13, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.3.243

Abstract
PDF

Ascaris suum eggs are inactivated by composting conditions; however, it is difficult to find functional changes in heat-treated A. suum eggs. Here, unembryonated A. suum eggs were incubated at 20℃, 50℃, and 70℃ in vitro, and the gene expression levels related to viability, such as eukaryotic translation initiation factor 4E (IF4E), phosphofructokinase 1 (PFK1), and thioredoxin 1 (TRX1), and to apoptosis, such as apoptosis-inducing factor 1 (AIF1) and cell death protein 6 (CDP6), were evaluated by real-time quantitative RT-PCR. No prominent morphological alterations were noted in the eggs at 20℃ until day 10. In contrast, the eggs developed rapidly, and embryonated eggs and hatched larvae began to die, starting on day 2 at 50℃ and day 1 at 70℃. At 20℃, IF4E, PFK1, and TRX1 mRNA expression was significantly increased from days 2-4; however, AIF1 and CDP6 mRNA expression was not changed significantly. IF4E, PFK1, and TRX1 mRNA expression was markedly decreased from day 2 at 50℃ and 70℃, whereas AIF1 and CDP6 mRNA expression was significantly increased. The expressions of HSP70 and HSP90 were detected for 9-10 days at 20℃, for 3-5 days at 50℃, and for 2 days at 70℃. Taken together, incremental heat increases were associated with the rapid development of A. suum eggs, decreased expression of genes related to viability, and earlier expression of apoptosis-related genes, and finally these changes of viability- and apoptosis-related genes of A. suum eggs were associated with survival of the eggs under temperature stress.

Citations

Citations to this article as recorded by

Environmental monitoring for Ascaris egg remediation: a critical review
Nathaniel I. Strong, Jack T. Andraka, Sung-Geun Woo, Sahar H. El Abbadi, Aaron J. Lewis, Sindy K. Y. Tang, Stephen P. Luby, Craig S. Criddle
Environmental Science and Pollution Research.2025; 32(17): 10688. CrossRef
Investigating cement-based surfaces as a sustainable flooring solution to improve Ascaris Suum egg removal and inactivation in low-resource settings
Claire E. Anderson, Suhi Hanif, Jason Hernandez, Yoshika Crider, Michael Lepech, Sarah L. Billington, Alexandria B. Boehm, Jade Benjamin-Chung, Vito Colella,
PLOS Neglected Tropical Diseases.2025; 19(10): e0012919. CrossRef
Chancen und Risiken der Nutzung genetischer Resistenzen gegen Infektionskrankheiten beim Schwein – eine Übersicht
Doris Höltig, Gerald Reiner
Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere.2022; 50(01): 46. CrossRef
Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii
Hui-Yu Chen, Yi-Sheng Cheng, Hsiu-Hui Shih
Veterinary Parasitology.2015; 212(3-4): 281. CrossRef
Effects of Some Pesticides on Development of Ascaris suum Eggs
Yong-Man Yu, Jin-Won Kim, Won-Seok Na, Young-Nam Youn, In-Wook Choi, Young-Ha Lee
The Korean Journal of Parasitology.2014; 52(1): 111. CrossRef

9,219 View
69 Download
Crossref

Original Articles

Anti-Apoptotic Effects of SERPIN B3 and B4 via STAT6 Activation in Macrophages after Infection with Toxoplasma gondii: Kyoung Ju Song, Hye-Jin Ahn, Ho-Woo Nam; Korean J Parasitol 2012;50(1):1-6.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.1

Abstract
PDF

Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.

Citations

Citations to this article as recorded by

Identification and functional analysis of a serine protease inhibitor using machine learning strategy
Heqian Zhang, Yaxin Wu, Yanran Zhu, Liangjun Ge, Jiaquan Huang, Zhiwei Qin
International Journal of Biological Macromolecules.2024; 265: 130852. CrossRef
Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells
Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh
Pathogens.2023; 12(2): 253. CrossRef
Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
Melissa Martins Oliveira, Camila Ramalho Bonturi, Bruno Ramos Salu, Maria Luiza Vilela Oliva, Renato Arruda Mortara, Cristina Mary Orikaza
Frontiers in Immunology.2022;[Epub] CrossRef
BEWO trophoblast cells and Toxoplasma gondii infection modulate cell death mechanisms in THP-1 monocyte cells by interference in the expression of death receptor and intracellular proteins
Andressa da Silva Castro, Mariana Bodini Angeloni, Bellisa de Freitas Barbosa, Renata Lima de Miranda, Samuel Cota Teixeira, Pâmela Mendonça Guirelli, Fernanda Chaves de Oliveira, Rafaela José da Silva, Priscila Silva Franco, Mayara Ribeiro, Iliana Claudi
Tissue and Cell.2021; 73: 101658. CrossRef
Transcriptional profiling of human macrophages during infection withBordetella pertussis
Denisa Petráčková, Mariam R. Farman, Fabian Amman, Irena Linhartová, Ana Dienstbier, Dilip Kumar, Jakub Držmíšek, Ivo Hofacker, Maria Eugenia Rodriguez, Branislav Večerek
RNA Biology.2020; 17(5): 731. CrossRef
Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
The Korean Journal of Parasitology.2020; 58(3): 249. CrossRef
Toxoplasma gondiiModulates the Host Cell Responses: An Overview of Apoptosis Pathways
Nour Mammari, Mohamad Adnan Halabi, Souha Yaacoub, Hilda Chlala, Marie-Laure Dardé, Bertrand Courtioux
BioMed Research International.2019; 2019: 1. CrossRef
Mechanisms of Human Innate Immune Evasion by Toxoplasma gondii
Tatiane S. Lima, Melissa B. Lodoen
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Strategies Developed by Toxoplasma gondii to Survive in the Host
Wanbo Zhu, Jingyang Li, Faustina Pappoe, Jilong Shen, Li Yu
Frontiers in Microbiology.2019;[Epub] CrossRef
Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella
Ling Lv, Bing Huang, Qiping Zhao, Zongping Zhao, Hui Dong, Shunhai Zhu, Ting Chen, Ming Yan, Hongyu Han
Parasites & Vectors.2018;[Epub] CrossRef
Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium
Linlu Qi, Mo Hu, Jiaqi Fu, Yanhua Liu, Mei Wu, Kaiwen Yu, Xiaoyun Liu
PROTEOMICS.2017;[Epub] CrossRef
Phosphoproteome of Toxoplasma gondii Infected Host Cells Reveals Specific Cellular Processes Predominating in Different Phases of Infection
Hai-Xia Wei, Ai-Yuan Chen, Cheng He, Hong-Juan Peng, Xiao-Shuang Feng
The American Journal of Tropical Medicine and Hygiene.2017; 97(1): 236. CrossRef
Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response
Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt
Frontiers in Immunology.2017;[Epub] CrossRef
Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1
Anne M. Brauweiler, Elena Goleva, Donald Y.M. Leung
Journal of Investigative Dermatology.2016; 136(3): 658. CrossRef
SERPINB3/B4 Contributes to Early Inflammation and Barrier Dysfunction in an Experimental Murine Model of Atopic Dermatitis
Umasundari Sivaprasad, Kayla G. Kinker, Mark B. Ericksen, Mark Lindsey, Aaron M. Gibson, Stacey A. Bass, Nicolas S. Hershey, Jingyuan Deng, Mario Medvedovic, Gurjit K. Khurana Hershey
Journal of Investigative Dermatology.2015; 135(1): 160. CrossRef
Trophoblast-macrophage crosstalk on human extravillous under Toxoplasma gondii infection
P.M. Guirelli, M.B. Angeloni, B.F. Barbosa, A.O. Gomes, A.S. Castro, P.S. Franco, R.J. Silva, J.G. Oliveira, O.A. Martins-Filho, J.R. Mineo, F. Ietta, E.A. Ferro
Placenta.2015; 36(10): 1106. CrossRef
Toxoplasma gondii inhibits apoptosis via a novel STAT3-miR-17–92-Bim pathway in macrophages
Yihong Cai, He Chen, Xuwei Mo, Yuanyuan Tang, Xiucai Xu, Aimei Zhang, Zhaorong Lun, Fangli Lu, Yong Wang, Jilong Shen
Cellular Signalling.2014; 26(6): 1204. CrossRef
The role of STAT-6 as a key transcription regulator in HeLa cell death induced by IFN-γ/TNF-α co-immobilized on nanoparticles
Zhibin Li, Yan-Qing Guan, Jun-Ming Liu
Biomaterials.2014; 35(18): 5016. CrossRef
Adaptive Evolution and Divergence of SERPINB3: A Young Duplicate in Great Apes
Sílvia Gomes, Patrícia I. Marques, Rune Matthiesen, Susana Seixas, Charaf Benarafa
PLoS ONE.2014; 9(8): e104935. CrossRef
Risk factors associated with seroprevalence of Neospora caninum in dogs from urban and rural areas of milk and coffee production in Minas Gerais state, Brazil
C. I. NOGUEIRA, L. P. MESQUITA, C. C. ABREU, K. Y. R. NAKAGAKI, J. N. SEIXAS, P. S. BEZERRA, C. M. B. M. ROCHA, A. M. GUIMARAES, A. P. PECONICK, M. S. VARASCHIN
Epidemiology and Infection.2013; 141(11): 2286. CrossRef
In-depth proteomic analysis of the human cerumen—A potential novel diagnostically relevant biofluid
Martin Andreas Feig, Elke Hammer, Uwe Völker, Nico Jehmlich
Journal of Proteomics.2013; 83: 119. CrossRef
Serpins, Immunity and Autoimmunity: Old Molecules, New Functions
Mariele Gatto, Luca Iaccarino, Anna Ghirardello, Nicola Bassi, Patrizia Pontisso, Leonardo Punzi, Yehuda Shoenfeld, Andrea Doria
Clinical Reviews in Allergy & Immunology.2013; 45(2): 267. CrossRef
STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage
Yihong Cai, He Chen, Lei Jin, Yibo You, Jilong Shen
Parasites & Vectors.2013;[Epub] CrossRef
Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
M.B. Angeloni, P.M. Guirelli, P.S. Franco, B.F. Barbosa, A.O. Gomes, A.S. Castro, N.M. Silva, O.A. Martins-Filho, T.W.P. Mineo, D.A.O. Silva, J.R. Mineo, E.A.V. Ferro
Placenta.2013; 34(11): 973. CrossRef
Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses
ZHI-LIANG DUAN, ZHI-BIN WANG, JIANG-LONG GUO, WEN-QUAN LIU, JUN HU, JING LI, SI-NA WANG, QIANG LI, JIN-SHENG WEN
International Journal of Oncology.2013; 42(4): 1482. CrossRef

10,952 View
93 Download
Crossref

Miltefosine-Induced Apoptotic Cell Death on Leishmania major and L. tropica Strains: Shahram Khademvatan, Mohammad Javad Gharavi, Fakher Rahim, Jasem Saki; Korean J Parasitol 2011;49(1):17-23.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.17

Abstract
PDF

The aim of this study was to assess the cytotoxic effects of various concentrations of miltefosine on Leishmania major (MRHO/IR/75/ER) and L. tropica (MHOM/IR/02/Mash10) promastigotes and to observe the programmed cell death features. The colorimetric MTT assay was used to find L. major and L. tropica viability and the obtained results were expressed as 50% inhibitory concentration (IC50). Also, 50% effective doses (ED50) for L. major and L. tropica amastigotes were also determined. Annexin-V FLUOS staining was performed to study the cell death properties of miltefosine using FACS analysis. Qualitative analysis of the total genomic DNA fragmentation was performed by agarose gel electrophoresis. Furthermore, to observe changes in cell morphology, promastigotes were examined using light microscopy. In both strains of L. major and L. tropica, miltefosine induced dose-dependent death with features of apoptosis, including cell shrinkage, DNA laddering, and externalization of phosphatidylserine. The IC50 was achieved at 22 ?M and 11 ?M for L. major and L. tropica after 48 hr of incubation, respectively. ED50 of L. major and L. tropica amastigotes were 5.7 ?M and 4.2 ?M, respectively. Our results indicate that miltefosine induces apoptosis of the causative agent of cutaneous leishmaniasis in a dose-dependent manner. Interestingly, L. major did not display any apoptotic changes when it was exposed to miltefosine in concentrations sufficient to kill L. tropica.

Citations

Citations to this article as recorded by

Miltefosine reduces coxsackievirus B3 lethality of mice with enhanced STAT3 activation
Chun Yu Zhang, Cheng-Huei Hung, Yi-Ling Hsiao, Tung-Miao Chang, Yu-Chieh Su, Li-Chiu Wang, Shih-Min Wang, Shun-Hua Chen
Antiviral Research.2024; 223: 105824. CrossRef
Appraisal of Chitosan-Coated Lipid Nano-Combination with Miltefosine and Albendazole in the Treatment of Murine Trichinellosis: Experimental Study with Evaluation of Immunological and Immunohistochemical Parameters
Asmaa F. Ibrahim, Sahar M. Selim, Dalia A. Shafey, Dina M. Sweed, Shaimaa A. Farag, Marwa A. Gouda
Acta Parasitologica.2024; 69(1): 929. CrossRef
Successful treatment of complex cutaneous leishmaniasis (L. tropica) in a 3‐year‐old Syrian boy with miltefosine
Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153. CrossRef
Erfolgreiche Therapie einer komplexen kutanen Leishmaniose (L. tropica) bei einem 3‐jährigen syrischen Jungen mit Miltefosin
Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153. CrossRef
Miltefosine induces reproductive toxicity during sperm capacitation by altering PI3K/AKT signaling pathway
Eun-Ju Jung, Woo-Jin Lee, Jeong-Won Bae, Woo-Sung Kwon
Environmental Toxicology and Pharmacology.2024; 111: 104565. CrossRef
Anti-leishmanial activity of Hypericum Scabrum extract against Leishmania major
Reza Saberi, Zaynab Jamshidzad, Elaheh Karimi, Jahangir Abdi, Razi Naserifar, Asad Mirzaei
AMB Express.2024;[Epub] CrossRef
A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry
Christian Florian Teh-Poot, Victor Manuel Dzul-Huchim, Jonathan M. Mercado, Liliana Estefanía Villanueva-Lizama, Maria Elena Bottazzi, Kathryn M. Jones, Francis T.F. Tsai, Julio Vladimir Cruz-Chan
Experimental Parasitology.2023; 249: 108519. CrossRef
The Antileishmanial Activity of Eugenol Associated with Lipid Storage Reduction Rather Than Membrane Properties Alterations
Kristelle Hughes, Thanh Binh Le, Patrick Van Der Smissen, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq, Joëlle Quetin-Leclercq
Molecules.2023; 28(9): 3871. CrossRef
Laurequinone, a Lead Compound against Leishmania
Sara García-Davis, Atteneri López-Arencibia, Carlos J. Bethencourt-Estrella, Desirée San Nicolás-Hernández, Ezequiel Viveros-Valdez, Ana R. Díaz-Marrero, José J. Fernández, Jacob Lorenzo-Morales, José E. Piñero
Marine Drugs.2023; 21(6): 333. CrossRef
In vitro efficacy of polymer coated miltefosine drug against leishmania tropica
Mehvish Khokhar, Muhammad Adnan Shereen, Momin Khan, Rahat Ullah Khan, Aamir Sohail, Imdad Ullah Khan, Inam Ullah Khan, Saadullah Khattak
Journal of Parasitic Diseases.2022; 46(2): 366. CrossRef
Tackling Drug Resistance and Other Causes of Treatment Failure in Leishmaniasis
Gert-Jan Wijnant, Franck Dumetz, Laura Dirkx, Dimitri Bulté, Bart Cuypers, Katrien Van Bocxlaer, Sarah Hendrickx
Frontiers in Tropical Diseases.2022;[Epub] CrossRef
The Effect of Curcumin on the Expression of INFγ, TNF-α, and iNOS Genes in PBMCs Infected with Leishmania major [MRHO/IR/75/ER]
Soheila Alinejad, Shahram Khademvatan, Shahla Amani, Negar Asadi, Khosrow Hazrati Tappeh, Elham Yousefi, Touraj Miandoabi
Infectious Disorders - Drug Targets.2022;[Epub] CrossRef
In Vitro Study of Cytotoxic Mechanisms of Alkylphospholipids and Alkyltriazoles in Acute Lymphoblastic Leukemia Models
Larissa de Oliveira Passos Jesus, Aline Aparecida de Souza, Heron Fernandes Vieira Torquato, Vanessa Silva Gontijo, Rossimirian Pereira de Freitas, Tarsis Ferreira Gesteira, Vivien Jane Coulson-Thomas, Ricardo José Soares Torquato, Aparecida Sadae Tanaka,
Molecules.2022; 27(23): 8633. CrossRef
Anti-leishmanial physalins—Phytochemical investigation, in vitro evaluation against clinical and MIL-resistant L. tropica strains and in silico studies
Saira Bano, Memoona Bibi, Saba Farooq, Humaira Zafar, Muniza Shaikh, Behram Khan Khoso, Sammer Yousuf, M. Iqbal Choudhary, Mohammad Shahid
PLOS ONE.2022; 17(11): e0274543. CrossRef
Leishmanicidal Activity and Ultrastructural Changes of Maslinic Acid Isolated from Hyptidendron canum
Jéssica Adriana Jesus, Márcia Dalastra Laurenti, Matheus Lopes Silva, João Henrique Ghilardi Lago, Luiz Felipe Domingues Passero, Valeria Sülsen
Evidence-Based Complementary and Alternative Medicine.2021; 2021: 1. CrossRef
A Novel Automated Framework for QSAR Modeling of Highly Imbalanced Leishmania High-Throughput Screening Data
Omar Casanova-Alvarez, Aliuska Morales-Helguera, Miguel Ángel Cabrera-Pérez, Reinaldo Molina-Ruiz, Christophe Molina
Journal of Chemical Information and Modeling.2021; 61(7): 3213. CrossRef
Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
Jasem Saki, Khalil Saki, Reza Arjmand
Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub] CrossRef
Une leishmaniose cutanée réfractaire : intérêt de la miltéfosine
C. Guerveno, K. Delavigne, A. Berry, G. Martin-Blondel, P. Delobel
Médecine et Maladies Infectieuses.2019; 49(4): 281. CrossRef
Natural compounds from plants controlling leishmanial growth via DNA damage and inhibiting trypanothione reductase and trypanothione synthetase: an in vitro and in silico approach
Shaila Mehwish, Huma Khan, Ashfaq Ur Rehman, Asif Ullah Khan, Mubarak Ali Khan, Obaid Hayat, Mansoor Ahmad, Abdul Wadood, Nazif Ullah
3 Biotech.2019;[Epub] CrossRef
Antileishmanial and cytotoxic activities of ionic surfactants compared to those of miltefosine
Lais Alonso, Éder Jeferson Souza Cardoso, Rodrigo Saar Gomes, Sebastião Antônio Mendanha, Miriam Leandro Dorta, Antonio Alonso
Colloids and Surfaces B: Biointerfaces.2019; 183: 110421. CrossRef
In vitro study of the efficacy of Solanum nigrum against Leishmania major
Christine N. Mutoro, Johnson K. Kinyua, Joseph K. Ng'ang'a, Daniel W. Kariuki, Johnstone M. Ingonga, Christopher O. Anjili
F1000Research.2018; 7: 1329. CrossRef
The cytotoxic activity of miltefosine against Leishmania and macrophages is associated with dynamic changes in plasma membrane proteins
Kelly Souza Fernandes, Paulo Eduardo Narcizo de Souza, Miriam Leandro Dorta, Antonio Alonso
Biochimica et Biophysica Acta (BBA) - Biomembranes.2017; 1859(1): 1. CrossRef
Antileishmanial and Immunomodulatory Activity of Allium sativum (Garlic)
Masoud Foroutan-Rad, Khosrow Hazrati Tappeh, Shahram Khademvatan
Journal of Evidence-Based Complementary & Alternative Medicine.2017; 22(1): 141. CrossRef
Effect of 1,2,3-triazole salts, non-classical bioisosteres of miltefosine, on Leishmania amazonensis
Pedro H.F. Stroppa, Luciana M.R. Antinarelli, Arturene M.L. Carmo, Jacy Gameiro, Elaine S. Coimbra, Adilson D. da Silva
Bioorganic & Medicinal Chemistry.2017; 25(12): 3034. CrossRef
Pomegranate (Punica granatum) Juice Shows Antioxidant Activity against Cutaneous Leishmaniasis-Induced Oxidative Stress in Female BALB/c Mice
Badriah Alkathiri, Manal El-Khadragy, Dina Metwally, Ebtesam Al-Olayan, Muhammed Bakhrebah, Ahmed Abdel Moneim
International Journal of Environmental Research and Public Health.2017; 14(12): 1592. CrossRef
Enterocin AS-48 as Evidence for the Use of Bacteriocins as New Leishmanicidal Agents
María Ángeles Abengózar, Rubén Cebrián, José María Saugar, Teresa Gárate, Eva Valdivia, Manuel Martínez-Bueno, Mercedes Maqueda, Luis Rivas
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Cytotoxic Activity ofHolothuria leucospilotaExtract againstLeishmania infantum In Vitro
Shahram Khademvatan, Alborz Eskandari, Jasem Saki, Masoud Foroutan-Rad
Advances in Pharmacological Sciences.2016; 2016: 1. CrossRef
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
Adriana Bezerra-Souza, Eduardo S. Yamamoto, Márcia D. Laurenti, Susan P. Ribeiro, Luiz Felipe D. Passero
Parasitology International.2016; 65(6): 702. CrossRef
New Approaches to Overcome Transport Related Drug Resistance in Trypanosomatid Parasites
Jose A. Garcia-Salcedo, Juan D. Unciti-Broceta, Javier Valverde-Pozo, Miguel Soriano
Frontiers in Pharmacology.2016;[Epub] CrossRef
Biological Activity of Coumarin Derivatives as Anti-Leishmanial Agents
Vineetha Mandlik, Sohan Patil, Ramanamurthy Bopanna, Sudipta Basu, Shailza Singh, Imtaiyaz Hassan
PLOS ONE.2016; 11(10): e0164585. CrossRef
Molecular docking and molecular dynamics simulation study of inositol phosphorylceramide synthase – inhibitor complex in leishmaniasis: Insight into the structure based drug design
Vineetha Mandlik, Shailza Singh
F1000Research.2016; 5: 1610. CrossRef
A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Paloma Tejera Nevado, Eugenia Bifeld, Katharina Höhn, Joachim Clos
Antimicrobial Agents and Chemotherapy.2016; 60(9): 5262. CrossRef
Molecular docking and molecular dynamics simulation study of inositol phosphorylceramide synthase – inhibitor complex in leishmaniasis: Insight into the structure based drug design
Vineetha Mandlik, Shailza Singh
F1000Research.2016; 5: 1610. CrossRef
Cinnamic Acid Bornyl Ester Derivatives from Valeriana wallichii Exhibit Antileishmanial In Vivo Activity in Leishmania major-Infected BALB/c Mice
Anita Masic, Ana Maria Valencia Hernandez, Sudipta Hazra, Jan Glaser, Ulrike Holzgrabe, Banasri Hazra, Uta Schurigt, Bhaskar Saha
PLOS ONE.2015; 10(11): e0142386. CrossRef
Evaluation of the effect of miltefosine on Trichomonas vaginalis
Débora Afonso Silva Rocha, Ivone de Andrade Rosa, Wanderley de Souza, Marlene Benchimol
Parasitology Research.2014; 113(3): 1041. CrossRef
In silico and in vitro comparative activity of novel experimental derivatives against Leishmania major and Leishmania infantum promastigotes
Shahram Khademvatan, Neda Adibpour, Alborz Eskandari, Saeed Rezaee, Mahmoud Hashemitabar, Fakher Rahim
Experimental Parasitology.2013; 135(2): 208. CrossRef
Pistagremic acid a new leishmanicidal triterpene isolated from Pistacia integerrima Stewart
Ghias Uddin, Abdur Rauf, Mohammad Arfan, Waliullah, Inamullah khan, Mumtaz Ali, Mazhar Taimur, Inaayat ur-Rehman, Samiullah
Journal of Enzyme Inhibition and Medicinal Chemistry.2012; 27(5): 646. CrossRef

12,525 View
99 Download
Crossref

Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan Parasite Entamoeba histolytica: Seobo Sim, Jae-Ran Yu, Young Ah Lee, Myeong Heon Shin; Korean J Parasitol 2010;48(4):285-290.
Published online December 16, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.4.285

Abstract
PDF

Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica. In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2. Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica.

Citations

Citations to this article as recorded by

The Src family kinases contribute to MII arrest maintenance in aging porcine oocytes
Kateřina Kheilová, Jaroslav Petr, Dalibor Řehák, Eva Chmelíková, Markéta Sedmíková
Theriogenology.2025; 248: 117614. CrossRef
The flip side of reactive oxygen species in the tropical disease‐Amoebiasis
Sakshi Desure, Arya Mallika, Mrinalini Roy, Anupam Jyoti, Sanket Kaushik, Vijay Kumar Srivastava
Chemical Biology & Drug Design.2021; 98(5): 930. CrossRef
Differential expression of ADAM15 and ADAM17 metalloproteases in the rat brain after severe hypobaric hypoxia and hypoxic preconditioning
Elena Rybnikova, Tatjana Gluschenko, Anasthasia Galeeva, Ekaterina Tulkova, Natalia N. Nalivaeva, Natalia Z. Makova, Anthony J. Turner, Mikhail Samoilov
Neuroscience Research.2012; 72(4): 364. CrossRef

8,415 View
64 Download
Crossref

Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate: Hyun-Ouk Song, Young-Su Lim, Sun-Joo Moon, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2010;48(1):1-7.
Published online March 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.1.1

Abstract
PDF

Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.

Citations

Citations to this article as recorded by

The role of neutrophils in the pathogenesis of Trichomonas vaginalis infection in pregnant women: A review
Emmanuel Ifeanyi Obeagu, Gideon Ikechukwu Okoroiwu, Nwanganga Ihuoma Ubosi, Getrude Uzoma Obeagu, Ebere Emilia Ayogu, Elham Elamin
Medicine.2025; 104(41): e45063. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth
María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila
Parasitology Research.2022; 121(5): 1355. CrossRef
Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis
F. J. Rangel-Mata, E. E. Ávila-Muro, J. E. Reyes-Martínez, L. M. Olmos-Ortiz, M. E. Brunck, L. A. Arriaga-Pizano, P. Cuéllar-Mata
Parasitology.2021; 148(13): 1624. CrossRef
Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells
Fei Fei Gao, Juan-Hua Quan, Min A. Lee, Wei Ye, Jae-Min Yuk, Guang-Ho Cha, In-Wook Choi, Young-Ha Lee
Parasites & Vectors.2021;[Epub] CrossRef
Molecular investigation of Trichomoniasis in women in Al-Muthana province /Iraq
H R J Al-abodi, K T M Al-Shaibani, E M Shaker
Journal of Physics: Conference Series.2019; 1234(1): 012078. CrossRef
Amaurocine: Anti- Trichomonas vaginalis protein produced by the basidiomycete Amauroderma camerarium
Mariana Duarte, Adriana Seixas, Maira Peres de Carvalho, Tiana Tasca, Alexandre José Macedo
Experimental Parasitology.2016; 161: 6. CrossRef
Trichomonas vaginalis induces cytopathic effect on human lung alveolar basal carcinoma epithelial cell line A549
Daile Meek C. Salvador-Membreve, Sonia D. Jacinto, Windell L. Rivera
Experimental Parasitology.2014; 147: 33. CrossRef
Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes
Juan-Hua Quan, Byung-Hun Kang, Guang-Ho Cha, Wei Zhou, Young-Bok Koh, Jung-Bo Yang, Heon-Jong Yoo, Min-A Lee, Jae-Sook Ryu, Heung-Tae Noh, Jaeyul Kwon, Young-Ha Lee, A. R. M. Ruhul Amin
PLoS ONE.2014; 9(10): e110659. CrossRef
Altered CD16 expression on vaginal neutrophils from women with vaginitis
Joziani Beghini, Paulo C. Giraldo, Ruth Riboldi, Rose L.G. Amaral, José Eleutério, Steven S. Witkin, Fernando Guimarães
European Journal of Obstetrics & Gynecology and Reproductive Biology.2013; 167(1): 96. CrossRef
Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products
Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin
Inflammation Research.2012; 61(2): 97. CrossRef
NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced byTrichomonas vaginalis-Derived Secretory Products
Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2011; 49(3): 291. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef

10,086 View
94 Download
Crossref

Brief Communications

Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidea): Soo-Ung Lee, Sun Huh; Korean J Parasitol 2006;44(3):233-237.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.233

Abstract
PDF

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

Citations

Citations to this article as recorded by

The genome of the sparganosis tapeworm Spirometra erinaceieuropaeiisolated from the biopsy of a migrating brain lesion
Hayley M Bennett, Hoi Ping Mok, Effrossyni Gkrania-Klotsas, Isheng J Tsai, Eleanor J Stanley, Nagui M Antoun, Avril Coghlan, Bhavana Harsha, Alessandra Traini, Diogo M Ribeiro, Sascha Steinbiss, Sebastian B Lucas, Kieren SJ Allinson, Stephen J Price, Thom
Genome Biology.2014;[Epub] CrossRef

8,207 View
56 Download
Crossref

Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica: Seobo Sim, Kyeong Ah Kim, Tai-Soon Yong, Soon-Jung Park, Kyung-il Im, Myeong Heon Shin; Korean J Parasitol 2004;42(4):205-208.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.205

Abstract
PDF

Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

Citations

Citations to this article as recorded by

Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica
Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
The state of art of neutrophil extracellular traps in protozoan and helminthic infections
César Díaz-Godínez, Julio C. Carrero
Bioscience Reports.2019;[Epub] CrossRef
Entamoeba histolytica Trophozoites Induce a Rapid Non-classical NETosis Mechanism Independent of NOX2-Derived Reactive Oxygen Species and PAD4 Activity
César Díaz-Godínez, Zayda Fonseca, Mario Néquiz, Juan P. Laclette, Carlos Rosales, Julio C. Carrero
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
Entamoeba histolytica induces human neutrophils to form NETs
J. Ventura‐Juarez, MR. Campos‐Esparza, J. Pacheco‐Yepez, J. A López‐Blanco, A. Adabache‐Ortíz, M. Silva‐Briano, R. Campos‐Rodríguez
Parasite Immunology.2016; 38(8): 503. CrossRef
Modulatory mechanisms of enterocyte apoptosis by viral, bacterial and parasitic pathogens
Andre G Buret, Amol Bhargava
Critical Reviews in Microbiology.2014; 40(1): 1. CrossRef
Anoikis potential of Entameba histolytica secretory cysteine proteases: Evidence of contact independent host cell death
Sudeep Kumar, Rajdeep Banerjee, Nilay Nandi, Abul Hasan Sardar, Pradeep Das
Microbial Pathogenesis.2012; 52(1): 69. CrossRef
In vivo programmed cell death of Entamoeba histolytica trophozoites in a hamster model of amoebic liver abscess
J. D. Villalba-Magdaleno, G. Perez-Ishiwara, J. Serrano-Luna, V. Tsutsumi, M. Shibayama
Microbiology.2011; 157(5): 1489. CrossRef
TGF‐β‐regulated tyrosine phosphatases induce lymphocyte apoptosis in Leishmania donovani‐infected hamsters
Rajdeep Banerjee, Sudeep Kumar, Abhik Sen, Ananda Mookerjee, Syamal Roy, Subrata Pal, Pradeep Das
Immunology & Cell Biology.2011; 89(3): 466. CrossRef
Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica
Seobo Sim, Jae-Ran Yu, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2010; 48(4): 285. CrossRef
Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica
Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
International Journal for Parasitology.2007; 37(11): 1209. CrossRef
Involvement of β2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica
Seobo Sim, Soon-Jung Park, Tai-Soon Yong, Kyung-Il Im, Myeong Heon Shin
Microbes and Infection.2007; 9(11): 1368. CrossRef
Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase‐3 and reducing Mcl‐1 expression
J. H. KANG, H. O. SONG, J. S. RYU, M. H. SHIN, J. M. KIM, Y. S. CHO, J. F. ALDERETE, M. H. AHN, D. Y. MIN
Parasite Immunology.2006; 28(9): 439. CrossRef
Toxoplasma gondii Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation
Ji-Young Kim, Myoung-Hee Ahn, Hye-Sun Jun, Jai-Won Jung, Jae-Sook Ryu, Duk-Young Min
Yonsei Medical Journal.2006; 47(6): 862. CrossRef

7,986 View
90 Download
Crossref

Original Articles

Effects of iNOS inhibitor on IFN-γ production and apoptosis of splenocytes in genetically different strains of mice infected with Toxoplasma gondii: Ki-Man Kang, Gye-Sung Lee, Jae-Ho Lee, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2004;42(4):175-183.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.175

Abstract
PDF

To evaluate the role of nitric oxide (NO) in IFN-γ production and apoptosis of splenocytes in genetically different strains of mice with toxoplasmosis, BALB/c (a toxoplasmosis resistant strain) and C57BL/6 (a toxoplasmosis susceptible strain) mice were infected with Toxoplasma gondii cysts orally and subsequently injected intraperitoneally with aminoguanidine, an iNOS inhibitor (AG; 35 mg/kg per mouse daily for 14 days). When BALB/c or C57BL/6 mice were infected with T. gondii without AG treatment, number of brain cysts, NO and IFN-γ production by splenocytes, and percentages of apoptotic splenocytes were increased compared to uninfected control mice without AG treatment. AG treatment increased the number of brain cysts, and reduced NO and IFN-γ production in T. gondii-infected C57BL/6 mice. In contrast, in T. gondii-infected BABL/c mice, the number of brain cysts, and NO and IFN-γ production of splenocytes was not altered by treatment with AG. However, the percentages of apoptotic splenocytes in T. gondii-infected BALB/c or C57BL/6 mice were not affected by AG treatment. These results suggest that NO modulates IFN-γ production in T. gondii-infected C57BL/6 mice, and that NO is involved in mediating a protective response in toxoplasmosis susceptible, but not resistant, mice strain during acute infection.

Citations

Citations to this article as recorded by

Effect of spiramycin versus aminoguanidine and their combined use in experimental toxoplasmosis
Marwa Omar, Beessa E. Abaza, Esraa Mousa, Shereen M. Ibrahim, Hayam E. Rashed, Tahani I. Farag
Journal of Parasitic Diseases.2021; 45(4): 1014. CrossRef
Recombinant TgHSP70 Immunization Protects against Toxoplasma gondii Brain Cyst Formation by Enhancing Inducible Nitric Oxide Expression
Paulo Czarnewski, Ester C. B. Araújo, Mário C. Oliveira, Tiago W. P. Mineo, Neide M. Silva
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
Pathophysiological mechanisms of diarrhea caused by theVibrio choleraeO1 El Tor variant: anin vivostudy in mice
Saravut Satitsri, Pawin Pongkorpsakol, Potjanee Srimanote, Varanuj Chatsudthipong, Chatchai Muanprasat
Virulence.2016; 7(7): 789. CrossRef
Oxidative stress and tryptophan degradation pattern of acute Toxoplasma gondii infection in mice
Ayse Basak Engin, Funda Dogruman-Al, Ugur Ercin, Bekir Celebi, Cahit Babur, Neslihan Bukan
Parasitology Research.2012; 111(4): 1725. CrossRef
Distinct roles for nitric oxide in resistant C57BL/6 and susceptible BALB/c mice to control Burkholderia pseudomallei infection
Katrin Breitbach, Patimaporn Wongprompitak, Ivo Steinmetz
BMC Immunology.2011;[Epub] CrossRef
MMHD [(S,E)-2-Methyl-1-(2-methylthiazol-4-yl) hexa-1,5-dien-ol], a Novel Synthetic Compound Derived From Epothilone, Suppresses Nuclear Factor-κB–Mediated Cytokine Expression in Lipopolysaccharide-Stimulated BV-2 Microglia
Na-Ra Jeon, Sushruta Koppula, Byung-Wook Kim, Su-Ho Park, Hyo-Won Lee, Dong-Kug Choi
Journal of Pharmacological Sciences.2010; 112(2): 158. CrossRef
The immunobiology of the innate response to Toxoplasma gondii
Catherine M. Miller, Nicola R. Boulter, Rowan J. Ikin, Nicholas C. Smith
International Journal for Parasitology.2009; 39(1): 23. CrossRef
Malondialdehyde, Glutathione, and Nitric Oxide Levels in Toxoplasma gondii Seropositive Patients
Ulku Karaman, Tuncay Celik, Tugba Raika Kiran, Cemil Colak, Nilgun Ulfet Daldal
The Korean Journal of Parasitology.2008; 46(4): 293. CrossRef
Toxoplasma gondii infection induces apoptosis in noninfected macrophages: role of nitric oxide and other soluble factors
Y. NISHIKAWA, O. KAWASE, O. VIELEMEYER, H. SUZUKI, K. A. JOINER, X. XUAN, H. NAGASAWA
Parasite Immunology.2007; 29(7): 375. CrossRef

8,892 View
114 Download
Crossref

Excretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis: Myeong-Heon Shin; Korean J Parasitol 2000;38(1):17-23.
Published online March 31, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.1.17

Abstract
PDF

Eosinophils are important effector cells in host defense against parasites. Excretory-secretory product (ESP) produced by helminthic worms plays important roles in the uptake of nutrients, migration in the host tissue, and in immune modulation. However, little is known about the ability of the ESP to directly trigger eosinophil apoptosis. This study investigated whether the ESP of newly excysted metacercariae of Paragonimus westermani could induce apoptosis in human eosinophils. Apoptosis was assayed by staining the cells with FITC-annexin V, and the cells were analyzed by flow cytometry. It was found that the ESP of newly excysted metacercariae of P. westermani induced a direct time- and concentration-dependent increase in the rate of constitutive apoptosis in mature human eosinophils. Eosinophil apoptosis was first apparent 3 hr after treatment with the ESP and continued to increase after 6 hr of incubation with respect to the cells cultured in the absence of the ESP. While only 2.8% of the eosinophils incubated in the medium for 3 hr were apoptotic, 7.6%, 10.9% and 22.6% of the eosinophils treated with 10, 30 and 100 ?g/ml ESP were apoptotic, respectively. This result suggests that the ESP of newly excysted metacercariae of P. westermani directly induce eosinophil apoptosis, which may be important for the survival of the parasites and the reduction of eosinophilic inflammation in vivo.

Citations

Citations to this article as recorded by

Gastric lesions in dolphins stranded along the Eastern Adriatic coast
J Hrabar, I Bočina, A Gudan Kurilj, M Đuras, I Mladineo
Diseases of Aquatic Organisms.2017; 125(2): 125. CrossRef
Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep
A. Escamilla, M.J. Bautista, R. Zafra, I.L. Pacheco, M.T. Ruiz, S. Martínez-Cruz, A. Méndez, A. Martínez-Moreno, V. Molina-Hernández, J. Pérez
Veterinary Parasitology.2016; 216: 84. CrossRef
Fasciola hepatica products induce apoptosis of peritoneal macrophages
Lorena Guasconi, Marianela C. Serradell, Diana T. Masih
Veterinary Immunology and Immunopathology.2012; 148(3-4): 359. CrossRef
Staphylococcus aureus Induces Eosinophil Cell Death Mediated by α-hemolysin
Lynne R. Prince, Kirstie J. Graham, John Connolly, Sadia Anwar, Robert Ridley, Ian Sabroe, Simon J. Foster, Moira K. B. Whyte, Malcolm James Horsburgh
PLoS ONE.2012; 7(2): e31506. CrossRef
A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils
Young-Bae Chung, Hirohito Kita, Myeong Heon Shin
The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef
Excretory-secretory products from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism
Marianela C. Serradell, Lorena Guasconi, Laura Cervi, Laura S. Chiapello, Diana T. Masih
Veterinary Immunology and Immunopathology.2007; 117(3-4): 197. CrossRef
Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells
Youngnam Jin, Jae-Chul Lee, In Young Choi, Eun A. Kim, Myeong Heon Shin, Won-Ki Kim
International Archives of Allergy and Immunology.2006; 139(1): 16. CrossRef
Cysteine Protease Secreted byParagonimus westermaniAttenuates Effector Functions of Human Eosinophils Stimulated with Immunoglobulin G
W. A. Petri, Myeong Heon Shin, Hirohito Kita, Hae Young Park, Ju Young Seoh
Infection and Immunity.2001; 69(3): 1599. CrossRef
Proteolytic activity of cysteine protease in excretory–secretory product of Paragonimus westermani newly excysted metacercariae pivotally regulates IL‐8 production of human eosinophils
Myeong Heon Shin, Soo Young Lee
Parasite Immunology.2000; 22(10): 529. CrossRef