Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis

, Yi, M R; , Shin, M H; , Leem, M H; , Ryu, J S; , Ahn, M H; , Min, D Y

doi:1990.28.1.25

Korean J Parasito > Volume 28(1):1990 > Article

Original Article


Korean J Parasitol. 1990 Mar;28(1):25-30. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1990.28.1.25
Copyright © 1990 by The Korean Society for Parasitology


Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis
M R Yi,M H Shin,M H Leem,J S Ryu,M H Ahn and D Y Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.


Abstract
The direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique is not so high. In this study enzyme-linked immunosorbent assay (ELISA) was employed for the detection of serum anti-T. vaginalis IgG and IgM antibodies from 30 vaginal trichomoniasis patients and 30 non-infected healthy persons. The results were as follows: 1. Serum ELISA-IgG value was 0.37 ± 0.134 (Mean ± S.D.) in vaginal trichomoniasis patients and 0.21 ± 0.054 in healthy controls (p < 0.005), and the sensitivity and specificity of ELISA for serum IgG antibody were 70.0% and 96.7%, respectively. 2. Serum ELISA-IgM value was 0.33 ± 0.177 (Mean ± S.D.) in vaginal trichomoniasis patients and 0.11 ± 0.051 in healthy controls (p < 0.005), and the sensitivity and specificity of ELISA for serum IgM antibody were 70.0% and 96.7%, respectively. 3. The ELISA-IgG values showed a significant correlation with ELISA-IgM values (r = 0.77, p < 0.005). With above results, it is assumed that ELISA is a reliable method for the diagnosis of T. vaginalis infection and simultaneous measurement of serum IgG and IgM with this technique is recommended.

Figures


	Fig. 1 Serum anti-T. vaginalis IgG responses in patients with trichomoniasis(•) and healthy controls(○). Broken line (…) means upper limit of normal optical density (mean+2 S.D.) for healthy controls.


	Fig. 2 Serum anti-T. vaginalis IgM responses in patients with trichomoniasis(•) and healthy controls(○). Broken line (…) means upper limit of normal optical density (mean+2 S.D.) for healthy controls.


	Fig. 3 Correlation between IgG-ELISA and IgM-ELISA values in trichomoniasis patients(•).

Tables


	Table 1 Serum IgG and IgM antibody levels in vaginal trichomoniasis patients by ELISA technique


	Table 2 Results of IgG-ELISA in vaginal trichomoniasis patients and healthy controls


	Table 3 Results of IgM-ELISA in vaginal trichomoniasis patients and healthy controls


	Table 4 Comparison of IgG-ELISA and IgM-ELISA in vaginal trichomoniasis


	Table 5 Comparison of IgG-ELISA and IgM-ELISA in healthy controls

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