Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the purity of antigens used. To solve this problem, many workers have tried to find species-specific components of antigens. The present study was performed to determine the antigenic profile of crude saline extracts of 3, 5, 8 and 12-week old P. westermani worms, which were collected from experimentally infected cats, based on SDS-PAGE and immunoblot technique. The results were as follows: 1. The SDS-PAGE showed at least 30 protein bands ranging from 229 kDa to 10 kDa molecular weight. The protein components of P. westermani changed chronologically during its developmental period. The 229 kDa band was recognized only in 12-week old worms (SEP12). 2. Analysis by ELISA showed a significant increase in antibody levels at 3 weeks in infected cats using crude saline extract antigens (SEP3, SEP5, SEP8, SEP12). 3. By EITB using SEP3 and SEP5, infected cats recognized major protein bands with molecular weight of 60, 35, 28, 25 or 21 kDa at 3-12 weeks of infection, and 3 additional antigens, 19, 13 and 10 kDa, were detected at 8-12 weeks of infections. 4. Using SEP8, 5 antigens, 91, 85, 31, 25 and 21 kDa, were consistently detected by all infected sera tested. In addition, 3 antigens of 19, 13 and 10 kDa were detected at 8-12 weeks of infection.(ABSTRACT TRUNCATED AT 250 WORDS)
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Protein composition and antigenicity of the tegument from Paragonimus westermani S I Kim, S Y Cho The Korean Journal of Parasitology.1993; 31(3): 269. CrossRef
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Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method H J Rim, S J Kim, I J Sun, J S Lee The Korean Journal of Parasitology.1992; 30(1): 1. CrossRef
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Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and centrifuging 24-week old adult worms at 10,000 rpm for 60 minutes in phosphate buffered saline (PBS, pH 7.2) containing phenyl methyl sulfonyl fluoride (PMSF). Gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed and blotted electrophoretically onto a sheet of nitrocellulose paper. The sheet was cut into strips and exposed to sera diluted 1: 200 with PBS. SDS-PAGE showed 26 protein bands ranging 229 to 10 kDa. Of them 229, 91, 60, 50, 35-31, 27, 25, 21, 17, 11 and 10 kDa components showed positive reaction with serum antibody of patients with P. westermani. Sera of patients infected with Clonorchis sinensis reacted with 35-31, 19, and 11 kDa bands. Human sera from cysticercosis and diphyllobothriasis cases showed non-specific cross reactions with 229, 35-31, 27, 25 and 17 kDa bands. Protein bands of 91, 60, 21 and 10 kDa showed strong positive reaction without cross reactions with sera from other helminthic infections.
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Protein composition and antigenicity of the tegument from Paragonimus westermani S I Kim, S Y Cho The Korean Journal of Parasitology.1993; 31(3): 269. CrossRef
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Molecular weight of major component proteins in crude saline extract of adult Paragonimus westermani Y Kong, WB Kim, SY Kang, SY Cho The Korean Journal of Parasitology.1991; 29(2): 113. CrossRef
Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies S Y Kang, Y Kong, S Y Cho The Korean Journal of Parasitology.1991; 29(4): 363. CrossRef
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Antigenic localities in the tissues of the young adult worm of Paragonimus westermani using immunogold labeling method O S Kwon, J S Lee, H J Rim, S J Kim The Korean Journal of Parasitology.1991; 29(1): 31. CrossRef
Paragonimus and paragonimiasis in Korea D W Choi The Korean Journal of Parasitology.1990; 28(Suppl): 79. CrossRef
Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique K H Joo, S C Hong, M S Chung, H J Rim The Korean Journal of Parasitology.1989; 27(1): 1. CrossRef
Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani S H Lee, S H Sung, J Y Chai The Korean Journal of Parasitology.1989; 27(2): 109. CrossRef
To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels were measured by enzyme-linked immunosorbent assay (ELISA). All but one patient who needed retreatment became symptom-free within a week. Antibody levels were dropped near to or below a cut-off absorbance (abs.) of 0.25 in varying intervals from 4 to 18 months. Of 9 patients who were retested within 3 months, 5 revealed temporary elevation of antibody level.
After the elevation, the levels began to decline slowly to negative ranges. If treated earlier after symptoms developed, the temporary elevation did not occur and intervals to negative conversion were shorter. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblot, antigen-antibody reactions in individual patient faded gradually without significant changes in reacting antigen bands.
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Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani Y Kong, J Y Chung, D H Yun, L S Kim, S Y Kang, A Ito, L Ma, S Y Cho The Korean Journal of Parasitology.1997; 35(3): 197. CrossRef
Protein composition and antigenicity of the tegument from Paragonimus westermani S I Kim, S Y Cho The Korean Journal of Parasitology.1993; 31(3): 269. CrossRef
Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani Y Kong, C Y Park, S Y Kang, S Y Cho The Korean Journal of Parasitology.1992; 30(2): 91. CrossRef
Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies S Y Kang, Y Kong, S Y Cho The Korean Journal of Parasitology.1991; 29(4): 363. CrossRef
Paragonimus and paragonimiasis in Korea D W Choi The Korean Journal of Parasitology.1990; 28(Suppl): 79. CrossRef
Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani S H Lee, S H Sung, J Y Chai The Korean Journal of Parasitology.1989; 27(2): 109. CrossRef
In an attempt to investigate the effect of Hymenolepis nana infection on immunological responses to sRBC in ICR strain of mice, cellular and humoral immune responses were chronologically monitored after sensitization with sRBC.
Mice weighing about 20 g were allocated into artificial and natural infection groups. The shell-free eggs of H. nana were inoculated into mice on the day 0 (initial) and day 10 in the former group, and praziquantel (25 mg/kg/day) was administered for 3 days to the one half of the mice at the 15th day after the first inoculation and to all of the mice in natural infection group. In artificial infection group, the delayed-type hypersensitivity (DTH) to sRBC was considerably decreased on the day 10 after the first inoculation, and then elevated gradually to normal.
Eosinophils in the peripheral blood increased slightly. The hemagglutinin (HA) and hemolysin (HE) titers during the early stage were shown to be more or less higher than those of control. Thereafter, the titers were returned to normal, followed by a transient decrease on the day 15 post-infection. The sRBC rosette and antibody-treated rosette-forming capacities on the day 15 post-infection were temporarily lowered but became higher thereafter. The mucosal mast cells (MMC) in the small intestine were gradually increased to make a peak on the day 10 post-infection and then maintained more or less at lower level. After praziquantel treatment, the DTH and the number of eosinophils were decreased slightly and the MMC number and sRBC rosette-forming capacity were considerably decreased. The titers of HA and HE and antibody-treated rosette-forming capacity, however, were elevated in general.
In natural infection group, the DTH, the number of eosinophils, and MMC which were elevated due to H. nana infection were gradually returned to normal after praziquantel treatment. The titers of HA and HE which were decreased by parasite infection were increased to normal after the treatment. However, the capacities of sRBC rosette or antibody-treated rosette formation were maintained at low levels in spite of the treatment. These results revealed that the immune responses to sRBC were significantly activated during H. nana infection, although they were transiently decreased during the days 10-15 post-infection.
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A case of tick bite by a spontaneously retreated Ixodes nipponensis B K Cho, H W Nam, S Y Cho, W K Lee The Korean Journal of Parasitology.1995; 33(3): 239. CrossRef
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A historical review and prospects of medical entomology research in Korea H I Ree The Korean Journal of Parasitology.1990; 28(Suppl): 145. CrossRef
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