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Volume 49(1); March 2011

Original Articles

Phospholipase Activities in Clinical and Environmental Isolates of Acanthamoeba
Abdul Matin, Suk-Yul Jung
Korean J Parasitol 2011;49(1):1-8.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.1

The pathogenesis and pathophysiology of Acanthamoeba infections remain incompletely understood. Phos-pholipases are known to cleave phospholipids, suggesting their possible involvement in the host cell plasma membrane disruption leading to host cell penetration and lysis. The aims of the present study were to determine phospholipase activities in Acanthamoeba and to determine their roles in the pathogenesis of Acanthamoeba. Using an encephalitis isolate (T1 genotype), a keratitis isolate (T4 genotype), and an environmental isolate (T7 genotype), we demonstrated that Acanthamoeba exhibited phospholipase A2 (PLA2) and phospholipase D (PLD) activities in a spectrophotometry-based assay. Interestingly, the encephalitis isolates of Acanthamoeba exhibited higher phospholipase activities as compared with the keratitis isolates, but the environmental isolates exhibited the highest phospholipase activities. Moreover, Acanthamoeba isolates exhibited higher PLD activities compared with the PLA2. Acanthamoeba exhibited optimal phospholipase activities at 37℃ and at neutral pH indicating their physiological relevance. The functional role of phospholipases was determined by in vitro assays using human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier. We observed that a PLD-specific inhibitor, i.e., compound 48/80, partially inhibited Acanthamoeba encephalitis isolate cytotoxicity of the host cells, while PLA2-specific inhibitor, i.e., cytidine 5'-diphosphocholine, had no effect on parasite-mediated HBMEC cytotoxicity. Overall, the T7 exhibited higher phospholipase activities as compared to the T4. In contract, the T7 exhibited minimal binding to, or cytotoxicity of, HBMEC.

Citations

Citations to this article as recorded by  Crossref logo
  • Detection of immunogenic protein components in excretion/secretion products of Acanthamoeba T5 using polyclonal antibodies
    Lissette Retana-Moreira, Elizabeth Abrahams-Sandí, Marco Ruiz-Campos, Johan Alvarado-Ocampo, Julián Castro, Jacob Lorenzo-Morales, Giovanni Sáenz-Arce, Antonio Osuna
    Memórias do Instituto Oswaldo Cruz.2025;[Epub]     CrossRef
  • Comprehensive characterization of extracellular vesicles produced by environmental (Neff) and clinical (T4) strains of Acanthamoeba castellanii
    Elisa Gonçalves Medeiros, Michele Ramos Valente, Leandro Honorato, Marina da Silva Ferreira, Susana Ruiz Mendoza, Diego de Souza Gonçalves, Lucas Martins Alcântara, Kamilla Xavier Gomes, Marcia Ribeiro Pinto, Ernesto S. Nakayasu, Geremy Clair, Isadora Fil
    mSystems.2024;[Epub]     CrossRef
  • Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
    Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
    Pathogens.2024; 13(2): 142.     CrossRef
  • Biological characteristics and pathogenicity of Acanthamoeba
    Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
    Francisco Sierra-López, Ismael Castelan-Ramírez, Dolores Hernández-Martínez, Lizbeth Salazar-Villatoro, David Segura-Cobos, Catalina Flores-Maldonado, Verónica Ivonne Hernández-Ramírez, Tomás Ernesto Villamar-Duque, Adolfo René Méndez-Cruz, Patricia Talam
    Microorganisms.2023; 11(11): 2762.     CrossRef
  • The gene expression and proteomic profiling of Acanthamoeba isolates
    Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta
    Experimental Parasitology.2023; 255: 108630.     CrossRef
  • Therapeutic agents and biocides for ocular infections by free-living amoebae of Acanthamoeba genus
    Linda Christian Carrijo-Carvalho, Viviane Peracini Sant'ana, Annette Silva Foronda, Denise de Freitas, Fabio Ramos de Souza Carvalho
    Survey of Ophthalmology.2017; 62(2): 203.     CrossRef
  • Single-Step Assembly of Multifunctional Poly(tannic acid)–Graphene Oxide Coating To Reduce Biofouling of Forward Osmosis Membranes
    Hanaa M. Hegab, Ahmed ElMekawy, Thomas G. Barclay, Andrew Michelmore, Linda Zou, Christopher P. Saint, Milena Ginic-Markovic
    ACS Applied Materials & Interfaces.2016; 8(27): 17519.     CrossRef
  • In vitro inhibition of protease-activated receptors 1, 2 and 4 demonstrates that these receptors are not involved in an Acanthamoeba castellanii keratitis isolate-mediated disruption of the human brain microvascular endothelial cells
    Junaid Iqbal, Komal Naeem, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
    Experimental Parasitology.2014; 145: S78.     CrossRef
  • 9,243 View
  • 82 Download
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A Ten-year Survey of Giardia Cysts in Drinking Water Supplies of Seoul, the Republic of Korea
Mok-Young Lee, Eun-Joo Cho, Jin-Hyo Lee, Sun-Hee Han, Yong-Sang Park
Korean J Parasitol 2011;49(1):9-15.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.9

To understand the distribution of Giardia cysts in drinking water supplies in Seoul, Korea, we collected water samples quarterly at 6 intakes in the Han River, its largest stream and 6 conventional water treatment plants (WTPs) serving drinking water, from 2000 to 2009. Giardia cysts in each of 10 L water were confirmed in 35.0% of intake water samples and the arithmetic mean was 1.65 cysts/10 L (range 0-35 cysts/10 L). The lowest cyst density was observed at Paldang and Kangbuk intakes, and the pollution level was higher at 4 intakes downstream. It seemed that these 4 intakes were under influence of Wangsuk stream at the end of which cysts were found in all samples with the mean of 140 cysts/10 L. The annual mean number of cysts was 0.21-4.21 cysts/10 L, and the cyst level at the second half of the 10 years was about 1/5 of that at first half on average. The cysts were more frequently found in winter, and their mean density was 3.74 cysts/10 L in winter and 0.80-1.08 cysts/10 L in other seasons. All finished water samples collected at 6 WTPs were negative for Giardia in each of 100 L sample for 10 years and cyst removal by physical process was average 2.9-log. It was concluded that conventional water treatment at 6 WTPs of Seoul appears to remove the cysts effectively under the present level of their source water. Domestic wastewater from the urban region could be an important source of Giardia pollution in the river.

Citations

Citations to this article as recorded by  Crossref logo
  • Improved recovery of Cryptosporidium and Giardia in water by optimizing immunomagnetic separation using acid and heat dissociation
    Eun-Sook Lee, Ji-Yeon Lee, Young-Ae Baek, Seok-Ju Cho
    Journal of Water and Health.2025; 23(6): 757.     CrossRef
  • Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in Korea
    Haeseung Lee, Dongmi Kwak
    Frontiers in Veterinary Science.2023;[Epub]     CrossRef
  • Occurrence of Giardia duodenalis in selected stations and tributary rivers of Laguna Lake, Philippines
    Mark Raymond A. Vejano, Laurice Beatrice Raphaelle O. dela Peña, Windell L. Rivera
    Environmental Monitoring and Assessment.2021;[Epub]     CrossRef
  • Multilocus genotyping of Giardia duodenalis from pigs in Korea
    Haeseung Lee, ByeongYeal Jung, Jun-Sik Lim, Min-Goo Seo, Seung-Hun Lee, Kwang-Ho Choi, Mi-Hye Hwang, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak
    Parasitology International.2020; 78: 102154.     CrossRef
  • Multilocus genotyping and risk factor analysis of Giardia duodenalis in dogs in Korea
    Ha-Young Kim, Haeseung Lee, Seung-Hun Lee, Min-Goo Seo, Seonju Yi, Jong Wan Kim, Chung Hyun Kim, Yu-Ran Lee, ByungJae So, Oh-Deog Kwon, Dongmi Kwak
    Acta Tropica.2019; 199: 105113.     CrossRef
  • Monitoring of Noxious Protozoa for Management of Natural Water Resources
    Young Yil Bahk, Pyo Yun Cho, Sung Kyu Ahn, Sangjung Park, Won Hwa Jheong, Yun-Kyu Park, Ho-Joon Shin, Sang-Seob Lee, Okjae Rhee, Tong-Soo Kim
    The Korean Journal of Parasitology.2018; 56(2): 205.     CrossRef
  • Prevalence and molecular characterisation of Giardia duodenalis in calves with diarrhoea
    S. H. Lee, D. VanBik, H. Y. Kim, A. Cho, J. W. Kim, J. W. Byun, J. K. Oem, S. I. Oh, D. Kwak
    Veterinary Record.2016; 178(25): 633.     CrossRef
  • A Case of Colitis by <i>Giardia lamblia</i> Infection with Atypical Presentation
    Soo Kyoung Choi, Youn Hee Lee, Jinhee Lee, Seon Ho Park, Mi Hee Kim, Ok-Ran Shin, Young-Seok Cho
    The Korean Journal of Medicine.2015; 89(3): 312.     CrossRef
  • Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
    Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
    Osong Public Health and Research Perspectives.2013; 4(3): 146.     CrossRef
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Miltefosine-Induced Apoptotic Cell Death on Leishmania major and L. tropica Strains
Shahram Khademvatan, Mohammad Javad Gharavi, Fakher Rahim, Jasem Saki
Korean J Parasitol 2011;49(1):17-23.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.17

The aim of this study was to assess the cytotoxic effects of various concentrations of miltefosine on Leishmania major (MRHO/IR/75/ER) and L. tropica (MHOM/IR/02/Mash10) promastigotes and to observe the programmed cell death features. The colorimetric MTT assay was used to find L. major and L. tropica viability and the obtained results were expressed as 50% inhibitory concentration (IC50). Also, 50% effective doses (ED50) for L. major and L. tropica amastigotes were also determined. Annexin-V FLUOS staining was performed to study the cell death properties of miltefosine using FACS analysis. Qualitative analysis of the total genomic DNA fragmentation was performed by agarose gel electrophoresis. Furthermore, to observe changes in cell morphology, promastigotes were examined using light microscopy. In both strains of L. major and L. tropica, miltefosine induced dose-dependent death with features of apoptosis, including cell shrinkage, DNA laddering, and externalization of phosphatidylserine. The IC50 was achieved at 22 ?M and 11 ?M for L. major and L. tropica after 48 hr of incubation, respectively. ED50 of L. major and L. tropica amastigotes were 5.7 ?M and 4.2 ?M, respectively. Our results indicate that miltefosine induces apoptosis of the causative agent of cutaneous leishmaniasis in a dose-dependent manner. Interestingly, L. major did not display any apoptotic changes when it was exposed to miltefosine in concentrations sufficient to kill L. tropica.

Citations

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  • Miltefosine reduces coxsackievirus B3 lethality of mice with enhanced STAT3 activation
    Chun Yu Zhang, Cheng-Huei Hung, Yi-Ling Hsiao, Tung-Miao Chang, Yu-Chieh Su, Li-Chiu Wang, Shih-Min Wang, Shun-Hua Chen
    Antiviral Research.2024; 223: 105824.     CrossRef
  • Appraisal of Chitosan-Coated Lipid Nano-Combination with Miltefosine and Albendazole in the Treatment of Murine Trichinellosis: Experimental Study with Evaluation of Immunological and Immunohistochemical Parameters
    Asmaa F. Ibrahim, Sahar M. Selim, Dalia A. Shafey, Dina M. Sweed, Shaimaa A. Farag, Marwa A. Gouda
    Acta Parasitologica.2024; 69(1): 929.     CrossRef
  • Successful treatment of complex cutaneous leishmaniasis (L. tropica) in a 3‐year‐old Syrian boy with miltefosine
    Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
    JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153.     CrossRef
  • Erfolgreiche Therapie einer komplexen kutanen Leishmaniose (L. tropica) bei einem 3‐jährigen syrischen Jungen mit Miltefosin
    Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
    JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153.     CrossRef
  • Miltefosine induces reproductive toxicity during sperm capacitation by altering PI3K/AKT signaling pathway
    Eun-Ju Jung, Woo-Jin Lee, Jeong-Won Bae, Woo-Sung Kwon
    Environmental Toxicology and Pharmacology.2024; 111: 104565.     CrossRef
  • Anti-leishmanial activity of Hypericum Scabrum extract against Leishmania major
    Reza Saberi, Zaynab Jamshidzad, Elaheh Karimi, Jahangir Abdi, Razi Naserifar, Asad Mirzaei
    AMB Express.2024;[Epub]     CrossRef
  • A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry
    Christian Florian Teh-Poot, Victor Manuel Dzul-Huchim, Jonathan M. Mercado, Liliana Estefanía Villanueva-Lizama, Maria Elena Bottazzi, Kathryn M. Jones, Francis T.F. Tsai, Julio Vladimir Cruz-Chan
    Experimental Parasitology.2023; 249: 108519.     CrossRef
  • The Antileishmanial Activity of Eugenol Associated with Lipid Storage Reduction Rather Than Membrane Properties Alterations
    Kristelle Hughes, Thanh Binh Le, Patrick Van Der Smissen, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq, Joëlle Quetin-Leclercq
    Molecules.2023; 28(9): 3871.     CrossRef
  • Laurequinone, a Lead Compound against Leishmania
    Sara García-Davis, Atteneri López-Arencibia, Carlos J. Bethencourt-Estrella, Desirée San Nicolás-Hernández, Ezequiel Viveros-Valdez, Ana R. Díaz-Marrero, José J. Fernández, Jacob Lorenzo-Morales, José E. Piñero
    Marine Drugs.2023; 21(6): 333.     CrossRef
  • In vitro efficacy of polymer coated miltefosine drug against leishmania tropica
    Mehvish Khokhar, Muhammad Adnan Shereen, Momin Khan, Rahat Ullah Khan, Aamir Sohail, Imdad Ullah Khan, Inam Ullah Khan, Saadullah Khattak
    Journal of Parasitic Diseases.2022; 46(2): 366.     CrossRef
  • Tackling Drug Resistance and Other Causes of Treatment Failure in Leishmaniasis
    Gert-Jan Wijnant, Franck Dumetz, Laura Dirkx, Dimitri Bulté, Bart Cuypers, Katrien Van Bocxlaer, Sarah Hendrickx
    Frontiers in Tropical Diseases.2022;[Epub]     CrossRef
  • The Effect of Curcumin on the Expression of INFγ, TNF-α, and iNOS Genes in PBMCs Infected with Leishmania major [MRHO/IR/75/ER]
    Soheila Alinejad, Shahram Khademvatan, Shahla Amani, Negar Asadi, Khosrow Hazrati Tappeh, Elham Yousefi, Touraj Miandoabi
    Infectious Disorders - Drug Targets.2022;[Epub]     CrossRef
  • In Vitro Study of Cytotoxic Mechanisms of Alkylphospholipids and Alkyltriazoles in Acute Lymphoblastic Leukemia Models
    Larissa de Oliveira Passos Jesus, Aline Aparecida de Souza, Heron Fernandes Vieira Torquato, Vanessa Silva Gontijo, Rossimirian Pereira de Freitas, Tarsis Ferreira Gesteira, Vivien Jane Coulson-Thomas, Ricardo José Soares Torquato, Aparecida Sadae Tanaka,
    Molecules.2022; 27(23): 8633.     CrossRef
  • Anti-leishmanial physalins—Phytochemical investigation, in vitro evaluation against clinical and MIL-resistant L. tropica strains and in silico studies
    Saira Bano, Memoona Bibi, Saba Farooq, Humaira Zafar, Muniza Shaikh, Behram Khan Khoso, Sammer Yousuf, M. Iqbal Choudhary, Mohammad Shahid
    PLOS ONE.2022; 17(11): e0274543.     CrossRef
  • Leishmanicidal Activity and Ultrastructural Changes of Maslinic Acid Isolated from Hyptidendron canum
    Jéssica Adriana Jesus, Márcia Dalastra Laurenti, Matheus Lopes Silva, João Henrique Ghilardi Lago, Luiz Felipe Domingues Passero, Valeria Sülsen
    Evidence-Based Complementary and Alternative Medicine.2021; 2021: 1.     CrossRef
  • A Novel Automated Framework for QSAR Modeling of Highly Imbalanced Leishmania High-Throughput Screening Data
    Omar Casanova-Alvarez, Aliuska Morales-Helguera, Miguel Ángel Cabrera-Pérez, Reinaldo Molina-Ruiz, Christophe Molina
    Journal of Chemical Information and Modeling.2021; 61(7): 3213.     CrossRef
  • Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
    Jasem Saki, Khalil Saki, Reza Arjmand
    Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub]     CrossRef
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    C. Guerveno, K. Delavigne, A. Berry, G. Martin-Blondel, P. Delobel
    Médecine et Maladies Infectieuses.2019; 49(4): 281.     CrossRef
  • Natural compounds from plants controlling leishmanial growth via DNA damage and inhibiting trypanothione reductase and trypanothione synthetase: an in vitro and in silico approach
    Shaila Mehwish, Huma Khan, Ashfaq Ur Rehman, Asif Ullah Khan, Mubarak Ali Khan, Obaid Hayat, Mansoor Ahmad, Abdul Wadood, Nazif Ullah
    3 Biotech.2019;[Epub]     CrossRef
  • Antileishmanial and cytotoxic activities of ionic surfactants compared to those of miltefosine
    Lais Alonso, Éder Jeferson Souza Cardoso, Rodrigo Saar Gomes, Sebastião Antônio Mendanha, Miriam Leandro Dorta, Antonio Alonso
    Colloids and Surfaces B: Biointerfaces.2019; 183: 110421.     CrossRef
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    Christine N. Mutoro, Johnson K. Kinyua, Joseph K. Ng'ang'a, Daniel W. Kariuki, Johnstone M. Ingonga, Christopher O. Anjili
    F1000Research.2018; 7: 1329.     CrossRef
  • The cytotoxic activity of miltefosine against Leishmania and macrophages is associated with dynamic changes in plasma membrane proteins
    Kelly Souza Fernandes, Paulo Eduardo Narcizo de Souza, Miriam Leandro Dorta, Antonio Alonso
    Biochimica et Biophysica Acta (BBA) - Biomembranes.2017; 1859(1): 1.     CrossRef
  • Antileishmanial and Immunomodulatory Activity of Allium sativum (Garlic)
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    Journal of Evidence-Based Complementary & Alternative Medicine.2017; 22(1): 141.     CrossRef
  • Effect of 1,2,3-triazole salts, non-classical bioisosteres of miltefosine, on Leishmania amazonensis
    Pedro H.F. Stroppa, Luciana M.R. Antinarelli, Arturene M.L. Carmo, Jacy Gameiro, Elaine S. Coimbra, Adilson D. da Silva
    Bioorganic & Medicinal Chemistry.2017; 25(12): 3034.     CrossRef
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    Badriah Alkathiri, Manal El-Khadragy, Dina Metwally, Ebtesam Al-Olayan, Muhammed Bakhrebah, Ahmed Abdel Moneim
    International Journal of Environmental Research and Public Health.2017; 14(12): 1592.     CrossRef
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    María Ángeles Abengózar, Rubén Cebrián, José María Saugar, Teresa Gárate, Eva Valdivia, Manuel Martínez-Bueno, Mercedes Maqueda, Luis Rivas
    Antimicrobial Agents and Chemotherapy.2017;[Epub]     CrossRef
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    Advances in Pharmacological Sciences.2016; 2016: 1.     CrossRef
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  • 12,523 View
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Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation
Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2011;49(1):25-31.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.25

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.

Citations

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  • Real-time detection of 3–8-µm microbial particles in sand filter effluent as a surrogate indicator of Cryptosporidium parvum oocysts
    Sandrine Boivin, Hiroshi Nagaoka, Takahiro Fujioka
    Water Research X.2025; 27: 100345.     CrossRef
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    Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert
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    Xin Gao, Jigang Yin, Dongqiang Wang, Xiaohui Li, Ying Zhang, Chenchen Wang, Yuanyuan Zhang, Guan Zhu
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
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    Takahiro Sekikawa, Kosuke Toshiki
    Water Supply.2015; 15(5): 1061.     CrossRef
  • 8,349 View
  • 92 Download
  • Crossref
Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand
Wanna Chaijaroenkul, Thanee Wongchai, Ronnatrai Ruangweerayut, Kesara Na-Bangchang
Korean J Parasitol 2011;49(1):33-38.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.33

Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pan™, Malaria Ag-Pf™, and Malaria Ag-Pv™ tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pf™ and Malaria Antigen Pf/Pan™ compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, and Malaria Antigen Pf/Pan™ were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pf™, Malaria Antigen Pf/Pan™, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pf™, Malaria Ag-Pv™, Malaria Antigen Pf/Pan™, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.

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  • Multiplexed quantitative proteomics provides mechanistic cues for malaria severity and complexity
    Vipin Kumar, Sandipan Ray, Shalini Aggarwal, Deeptarup Biswas, Manali Jadhav, Radha Yadav, Sanjeev V. Sabnis, Soumaditya Banerjee, Arunansu Talukdar, Sanjay K. Kochar, Suvin Shetty, Kunal Sehgal, Swati Patankar, Sanjeeva Srivastava
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    Endale TADESSE, Bereket WORKALEMAHU, Techalew SHIMELIS
    Revista do Instituto de Medicina Tropical de São Paulo.2016;[Epub]     CrossRef
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    Shyamapada Mandal
    Asian Pacific Journal of Tropical Disease.2014; 4: S13.     CrossRef
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    Pimwan Thongdee, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang
    The Korean Journal of Parasitology.2014; 52(4): 377.     CrossRef
  • Rapid diagnostic tests for diagnosing uncomplicated non-falciparum or Plasmodium vivax malaria in endemic countries
    Katharine Abba, Amanda J Kirkham, Piero L Olliaro, Jonathan J Deeks, Sarah Donegan, Paul Garner, Yemisi Takwoingi
    Cochrane Database of Systematic Reviews.2014;[Epub]     CrossRef
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Anisakis simplex Larvae: Infection Status in Marine Fish and Cephalopods Purchased from the Cooperative Fish Market in Busan, Korea
Seon Hee Choi, Jung Kim, Jin Ok Jo, Min Kyung Cho, Hak Sun Yu, Hee Jae Cha, Mee Sun Ock
Korean J Parasitol 2011;49(1):39-44.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.39

The infection status of marine fish and cephalopods with Anisakis simplex third stage larva (L3) was studied over a period of 1 year. A total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the Cooperative Fish Market in Busan, Korea, from August 2006 to July 2007. They were examined for A. simplex L3 from the whole body cavity, viscera, and muscles. A. simplex L3 were confirmed by light microscopy. The overall infection rate reached 34.3%, and average 17.1 larvae were parasitized per infected fish. Fish that recorded the highest infection rate was Lophiomus setigerus (100%), followed by Liparis tessellates (90%), Pleurogrammus azonus (90%), and Scomber japonicus (88.7%). The intensity of infection was the highest in Gadus macrocephalus (117.7 larvae per fish), followed by S. japonicus (103.9 larvae) and L. setigerus (54.2 larvae). Although abundance of A. simplex L3 was not seasonal in most of the fish species, 10 of the 16 selected species showed the highest abundance in February and April. A positive correlation between the intensity of L3 infection and the fish length was obvious in S. japonicus and G. macrocephalus. It was likely that A. simplex L3 are more frequently infected during the spring season in some species of fish. Our study revealed that eating raw or undercooked fish or cephalopods could still be a source of human infection with A. simplex L3 in Korea.

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  • Comparative study of Taqman-based qPCR assay for the detection of Anisakis simplex and Pseudoterranova decipiens
    Mi-Gyeong Kim, Min Ji Hong, Doo Won Seo, Hyun Mi Jung, Hyun-Ja Han, Seung Hwan Kim, Insun Joo, Elingarami Sauli
    PLOS One.2025; 20(4): e0320724.     CrossRef
  • A critical review of anisakidosis cases occurring globally
    Shokoofeh Shamsi, Diane P. Barton
    Parasitology Research.2023; 122(8): 1733.     CrossRef
  • Larval ascaridoid nematodes in horned and musky octopus (Eledone cirrhosa and E. moschata) and longfin inshore squid (Doryteuthis pealeii): Safety and quality implications for cephalopod products sold as fresh on the Italian market
    L. Guardone, E. Bilska-Zając, A. Giusti, R. Malandra, T. Cencek, A. Armani
    International Journal of Food Microbiology.2020; 333: 108812.     CrossRef
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    Eevi Savola, Dieter Ebert
    BMC Ecology.2019;[Epub]     CrossRef
  • Seafood-Borne Parasitic Diseases: A “One-Health” Approach Is Needed
    Shokoofeh Shamsi
    Fishes.2019; 4(1): 9.     CrossRef
  • Endosonographic Findings and the Natural Course of Chronic Gastric Anisakiasis: A Single-Center Experience
    Eun Young Park, Dong Hoon Baek, Gwang Ha Kim, Bong Eun Lee, So-Jeong Lee, Do Youn Park
    Gastroenterology Research and Practice.2018; 2018: 1.     CrossRef
  • Hiện trạng nhiễm ký sinh trùng trên cá bớp (Rachycentron canadum) nuôi lồng ở tỉnh Kiên Giang
    Từ Thanh Dung, Phan Văn Út, Nguyễn Bảo Trung
    Can Tho University Journal of Science.2017; 51: 106.     CrossRef
  • Occurrence and molecular identification of anisakid nematodes isolated from Pacific cod ( Gadus macrocephalus ) caught off Korea
    Chan-Hyeok Jeon, Eko Setyobudi, Jeong-Ho Kim
    Food Control.2016; 69: 100.     CrossRef
  • Inactivation of Anisakis simplex L3 in the flesh of white spotted conger (Conger myriaster) by high hydrostatic pressure and its effect on quality
    Ki-Hoon Lee, Shin Young Park, Sang-Do Ha
    Food Additives & Contaminants: Part A.2016; 33(6): 1010.     CrossRef
  • Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Marine Fishes
    Woo Joo Lee, Dong Joo Seo, Hyejin Oh, Su Been Jeon, Day Jung, Changsun Choi
    Journal of Food Protection.2016; 79(5): 789.     CrossRef
  • <i>Anisakis pegreffii</i> Larvae in Sea Eels (<i>Astroconger myriaster</i>) from the South Sea, Republic of Korea
    Jaeeun Cho, Hyemi Lim, Bong-Kwang Jung, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(3): 349.     CrossRef
  • Molecular Analysis ofAnisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea
    Woon-Mok Sohn, Jung-Mi Kang, Byoung-Kuk Na
    The Korean Journal of Parasitology.2014; 52(4): 383.     CrossRef
  • Molecular identification of anisakid nematodes third stage larvae isolated from common squid (Todarodes pacificus) in Korea
    Eko Setyobudi, Chan-Hyeok Jeon, Kwangho Choi, Sung Il Lee, Chung Il Lee, Jeong-Ho Kim
    Ocean Science Journal.2013; 48(2): 197.     CrossRef
  • Anisakidosis in Korea; Changes Over the Last Decade
    Hee Jae Cha, Mee Sun Ock
    Kosin Medical Journal.2012; 27(2): 73.     CrossRef
  • Larval Anisakid Infections in Marine Fish from Three Sea Areas of the Republic of Korea
    Shin-Hyeong Cho, Sang-Eun Lee, Ok-Hee Park, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2012; 50(4): 295.     CrossRef
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    Andrea Armani, Lorenzo Castigliego, Lara Tinacci, Gabriele Gandini, Daniela Gianfaldoni, Alessandra Guidi
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  • Laryngeal Anisakiasis
    Seo Young Kwak, Yeo‐Hoon Yoon
    Otolaryngology–Head and Neck Surgery.2012; 147(3): 588.     CrossRef
  • Seroprevalence of Antibodies againstAnisakis simplexLarvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
    Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock
    The Korean Journal of Parasitology.2011; 49(2): 139.     CrossRef
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Influencing Factors for Cure of Clonorchiasis by Praziquantel Therapy: Infection Burden and CYP3A5 Gene Polymorphism
Chung Hyeon Kim, Jeong-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Korean J Parasitol 2011;49(1):45-49.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.45

Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2±3,600.0 vs 4,998.5±7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs≥1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs≥1,000.

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  • Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control
    Men-Bao Qian, Jennifer Keiser, Jürg Utzinger, Xiao-Nong Zhou, Graeme N. Forrest
    Clinical Microbiology Reviews.2024;[Epub]     CrossRef
  • Efficacy and Safety of Tribendimidine Against Clonorchis sinensis
    Men-Bao Qian, Peiling Yap, Yi-Chao Yang, Hai Liang, Zhi-Hua Jiang, Wei Li, Yu-Guang Tan, Hui Zhou, Jürg Utzinger, Xiao-Nong Zhou, Jennifer Keiser
    Clinical Infectious Diseases.2013; 56(7): e76.     CrossRef
  • Efficacy of Praziquantel against Schistosoma mekongi and Opisthorchis viverrini: A Randomized, Single-Blinded Dose-Comparison Trial
    Leonore Lovis, Tippi K. Mak, Khampheng Phongluxa, Phonepasong Ayé Soukhathammavong, Youthanavanh Vonghachack, Jennifer Keiser, Penelope Vounatsou, Marcel Tanner, Christoph Hatz, Jürg Utzinger, Peter Odermatt, Kongsap Akkhavong, Banchob Sripa
    PLoS Neglected Tropical Diseases.2012; 6(7): e1726.     CrossRef
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Gastrointestinal Myiasis by Larvae of Sarcophaga sp. and Oestrus sp. in Egypt: Report of Cases, and Endoscopical and Morphological Studies
Azza K. Ahmad, Ekhlas H. Abdel-Hafeez, Madiha Makhloof, Ehab M. Abdel-Raheem
Korean J Parasitol 2011;49(1):51-57.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.51

Four cases of gastric or intestinal myiasis are reported. The cases contain 2 males (1 child 10 years old, and 1 adult 40 years old) and 2 females (1 girl 18 years old, and 1 adult 50 years old) from Minia Governorate, Southern Egypt. Three of them, including cases no. 1, 3, and 4, were gastric myiasis, and complained of offensive hematemesis of bright red blood. Minute moving worms, larvae of the fly, were found in the vomitus. On the other hand, case no. 2 had intestinal myiasis, and complained of abdominal distention, nausea, vomiting, and diarrhea. The stool of case 2 was mixed with blood, and minute moving worms were observed in the stool. Endoscopy was performed to explore any pathological changes in the stomach of the patients. The larvae were collected and studied macroscopically, microscopically, and us-ing a scanning electron microscope (SEM) to identify their species. Three different types of larvae were identified. The larvae isolated from case 1 were diagnosed as the second stage larvae of Sarcophaga species, and the larvae isolated from case 2 were the third stage larvae of Sarcophaga species. On the other hand, the larvae isolated from cases 3 and 4 were diagnosed as the third stage larvae of Oestrus species.

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  • Nasal Myiasis in an Immunocompetent Child With Cerebral Palsy: A Case Report
    Mohamed Bouallou, Mohammed Amine Rabhi, Achraf Sbai, Drissia Benfadil, Azzedine Lachkar, Fahd El Ayoubi
    Cureus.2025;[Epub]     CrossRef
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    Sahar Azarmi, Kamran Akbarzadeh, Ali Ekrami, Zahra Sheikh, Omid Dehghan
    BMC Infectious Diseases.2024;[Epub]     CrossRef
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    Beenish Mirza, Mohammad Amir
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    Mônica Salazar-Souza, José Mario d’Almeida, Marcelo Salabert Gonzalez, Valéria Magalhães Aguiar, Denise Feder
    Parasitology Research.2023; 122(9): 1983.     CrossRef
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    Xiu-Qi Chen, Kang-Ling Zhang, Qing-Wen Shan
    Journal of Tropical Pediatrics.2021;[Epub]     CrossRef
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    P. P. Jayawardana, T. C. Yahathugoda, Ozgur Kasapcopur
    Case Reports in Pediatrics.2021; 2021: 1.     CrossRef
  • AURAL MYIASIS, AN UNUSUAL PRESENTATION
    Anupam Batra, Anita Singhare, Vishal R. Munjal
    PARIPEX INDIAN JOURNAL OF RESEARCH.2021; : 73.     CrossRef
  • Nasal myiasis among pediatric patients: Our experiences
    SantoshKumar Swain, Rachita Sarangi, JatindraNath Mohanty
    Medical Journal of Dr. D.Y. Patil Vidyapeeth.2020; 13(1): 48.     CrossRef
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    Simon K. Kuria, Adebola O. Oyedeji
    Acta Tropica.2020; 210: 105590.     CrossRef
  • The family Oestridae in Egypt and Saudi Arabia (Diptera, Oestroidea)
    Magdi S. A. El-Hawagry, Mahmoud S. Abdel-Dayem, Hathal M. Al Dhafer
    ZooKeys.2020; 947: 113.     CrossRef
  • A brief review of forensically important flesh flies (Diptera: Sarcophagidae)
    Lipin Ren, Yanjie Shang, Wei Chen, Fanming Meng, Jifeng Cai, Guanghui Zhu, Lushi Chen, Yong Wang, Jianqiang Deng, Yadong Guo
    Forensic Sciences Research.2018; 3(1): 16.     CrossRef
  • Intestinal Myiasis Caused bySarcophagaspp. in Cusco, Peru: A Case Report and Review of the Literature
    Priscilla Ly, Adiel Aizenberg, Taylor Martin, Martha Lopez, Miguel Arturo Saldaña, Grant Leslie Hughes, Miguel Mauricio Cabada
    Case Reports in Infectious Diseases.2018; 2018: 1.     CrossRef
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    Apollo Medicine.2018; 15(3): 128.     CrossRef
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    Naoki Watanabe, Tomohiro Kato, Yoshie Ichiyanagi, Yuko Kubota, Shigeyuki Sugie, Teruki Kadosaka, Takafumi Naiki, Takuji Tanaka
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  • Human wound myiasis caused by Phormia regina and Sarcophaga haemorrhoidalis in Minia Governorate, Egypt
    Ekhlas H. Abdel-Hafeez, Rabie M. Mohamed, Usama S. Belal, Ahmed M. Atiya, Masaya Takamoto, Fumie Aosai
    Parasitology Research.2015; 114(10): 3703.     CrossRef
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    Indian Journal of Medical Microbiology.2012; 30(3): 332.     CrossRef
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Seasonal Prevalence of Mosquitoes, Including Vectors of Brugian Filariasis, in Southern Islands of the Republic of Korea
Hyeng-Il Cheun, Shin-Hyeong Cho, Hee-Il Lee, E-Hyun Shin, Jong-Soo Lee, Tong-Soo Kim, Won-Ja Lee
Korean J Parasitol 2011;49(1):59-64.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.59

A survey of mosquitoes, including the vector status of Brugia malayi filariasis and their relative larval density, was conducted from 2002 to 2005 at several southern remote islands of Jeollanam-do (province), Gyeongsangnam-do, and Jeju-do, Korea, where filariasis was previously endemic. Overall, a total of 9 species belonging to 7 genera were collected. Ochlerotatus togoi (formerly known as Aedes togoi), Anopheles (Hyrcanus) group, and Culex pipiens were the predominant species captured at all areas. Oc. togoi larvae were most frequently collected at salinity levels <0.5% during June and July, with densities decreasing sharply during the rainy season in August. The most likely explanation for the eradication of filariasis in these areas is suggested to be an aggressive treatment program executed during the 1970s and the 1990s. However, high prevalence of the vector mosquitoes may constitute a potential risk for reemerging of brugian filariasis in these areas.

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    Budi Mulyaningsih, Sitti Rahmah Umniyati, Suwarno Hadisusanto, Erwin Edyansyah
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    Journal of Insect Science.2020;[Epub]     CrossRef
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    Sevidzem Silas Lendzele, Raymond Tchawe, Zinga-Koumba Roland, Mamoudou Abdoulmoum, Ndjonka Dieudonne, Mavoungou Jacques Francois
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    Parasites & Vectors.2018;[Epub]     CrossRef
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    Marcus S. C. Blagrove, Cyril Caminade, Elisabeth Waldmann, Elizabeth R. Sutton, Maya Wardeh, Matthew Baylis, Christopher M. Barker
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    Kui-Hyun Yoon
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    Hyun-Ye Jo, Dong-Kun Yang, Ha-Hyun Kim, Sung-Suk Choi, Kyung-Suk Kang, Sun-Ju Yang, Young-Jin Yang, In-Soo Cho
    Journal of Bacteriology and Virology.2015; 45(3): 235.     CrossRef
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    Teiji Sota, Peter Belton, Michelle Tseng, Hoi Sen Yong, Motoyoshi Mogi, Igor Mokrousov
    PLOS ONE.2015; 10(6): e0131230.     CrossRef
  • Peritrophic matrix formation and Brugia malayi microfilaria invasion of the midgut of a susceptible vector, Ochlerotatus togoi (Diptera: Culicidae)
    Narissara Jariyapan, Atiporn Saeung, Nuchpicha Intakhan, Wetpisit Chanmol, Sriwatapron Sor-suwan, Benjarat Phattanawiboon, Kritsana Taai, Wej Choochote
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  • Environmental Forcing Shapes Regional House Mosquito Synchrony in a Warming Temperate Island
    Luis Fernando Chaves, Yukiko Higa, Su Hyun Lee, Ji Yeon Jeong, Sang Taek Heo, Miok Kim, Noboru Minakawa, Keun Hwa Lee
    Environmental Entomology.2013; 42(4): 605.     CrossRef
  • Surveillance and Vector Control of Lymphatic Filariasis in the Republic of Korea
    Shin Hyeong Cho, Da Won Ma, Bo Ra Koo, Hee Eun Shin, Wook Kyo Lee, Byong Suk Jeong, Chaeshin Chu, Won Ja Lee, Hyeng Il Cheun
    Osong Public Health and Research Perspectives.2012; 3(3): 145.     CrossRef
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Case Reports

A Case of Biliary Fascioliasis by Fasciola gigantica in Turkey
Vedat Goral, Senem Senturk, Omer Mete, Mutallib Cicek, Berat Ebik, Be?ir Kaya
Korean J Parasitol 2011;49(1):65-68.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.65

A case of Fasciola gigantica-induced biliary obstruction and cholestasis is reported in Turkey. The patient was a 37- year-old woman, and suffered from icterus, ascites, and pain in her right upper abdominal region. A total of 7 living adult flukes were recovered during endoscopic retrograde cholangiopancreatography (ERCP). A single dose of triclabendazole was administered to treat possible remaining worms. She was living in a village of southeast of Anatolia region and had sheeps and cows. She had the history of eating lettuce, mallow, dill, and parsley without washing. This is the first case of fascioliasis which was treated via endoscopic biliary extraction during ERCP in Turkey.

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    Santiago Mas-Coma, M. Adela Valero, M. Dolores Bargues
    Clinical Microbiology Reviews.2022;[Epub]     CrossRef
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    Sarah Gabriël, Pierre Dorny, Ganna Saelens, Veronique Dermauw
    Foods.2022; 12(1): 142.     CrossRef
  • Triclabendazole in the treatment of human fascioliasis: a review
    Preetam Gandhi, Esther K Schmitt, Chien-Wei Chen, Sanjay Samantray, Vinay Kumar Venishetty, David Hughes
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2019; 113(12): 797.     CrossRef
  • Fasciola hepatica in the common bile duct: spyglass visualization and endoscopic extraction
    Edson Guzmán Calderón, Augusto Vera Calderón, Ramiro Díaz Ríos, Ronald Arcana López, Edgar Alva Alva
    Revista Española de Enfermedades Digestivas.2018;[Epub]     CrossRef
  • Human fascioliasis by Fasciola hepatica: the first case report in Nepal
    Ranjit Sah, Shusila Khadka, Mohan Khadka, Dipesh Gurubacharya, Jeevan Bahadur Sherchand, Keshab Parajuli, Niranjan Prasad Shah, Hari Prasad Kattel, Bharat Mani Pokharel, Basista Rijal
    BMC Research Notes.2017;[Epub]     CrossRef
  • Biliary Fasciola gigantica infestation in a nonendemic area — An intraoperative surprise
    Prema Menon, Amit Kumar Sinha, Katragadda Lakshmi Narasimha Rao, Sumeeta Khurana, Sadhana Lal, Babu Ram Thapa
    Journal of Pediatric Surgery.2015; 50(11): 1983.     CrossRef
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    S. Nguyen, S. Amer, M. Ichikawa, T. Itagaki, Y. Fukuda, Y. Nakai
    Parasite.2012; 19(1): 85.     CrossRef
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  • 86 Download
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A Pulmonary Paragonimiasis Case Mimicking Metastatic Pulmonary Tumor
Ki Uk Kim, Kwangha Lee, Hye-Kyung Park, Yeon Joo Jeong, Hak Sun Yu, Min Ki Lee
Korean J Parasitol 2011;49(1):69-72.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.69

Pulmonary paragonimiasis is a relatively rare cause of lung disease revealing a wide variety of radiologic findings, such as air-space consolidation, nodules, and cysts. We describe here a case of pulmonary paragonimiasis in a 27-year-old woman who presented with a 2-month history of cough and sputum. Based on chest computed tomography (CT) scans and fluorodeoxyglucose positron emission tomography (FDG-PET) findings, the patient was suspected to have a metastatic lung tumor. However, she was diagnosed as having Paragonimus westermani infection by an immunoserological examination using ELISA. Follow-up chest X-ray and CT scans after chemotherapy with praziquantel showed an obvious improvement. There have been several reported cases of pulmonary paragonimiasis mimicking lung tumors on FDG-PET. However, all of them were suspected as primary lung tumors. To our knowledge, this patient represents the first case of paragonimiasis mimicking metastatic lung disease on FDG-PET CT imaging.

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    Min Seo
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A Paragonimiasis Patient with Allergic Reaction to Praziquantel and Resistance to Triclabendazole: Successful Treatment after Desensitization to Praziquantel
Sun Young Kyung, Yong Kyun Cho, Yu Jin Kim, Jeong-Woong Park, Sung Hwan Jeong, Jae-Ik Lee, Yon Mi Sung, Sang Pyo Lee
Korean J Parasitol 2011;49(1):73-77.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.73

Paragonimiasis is an infectious disease caused by trematodes of the genus Paragonimus. This trematode can be treated successfully with praziquantel in more than 90% of the cases. Although praziquantel is generally well tolerated, anaphylactic reactions to this drug have been reported in a few cases. We report here a 46-year-old Korean female with paragonimiasis, presumed to be due to Paragonimus westermani, who displayed an allergic reaction to praziquantel and resistance to triclabendazole treatment. The patient was successfully treated with praziquantel following a rapid desensitization procedure. Desensitization to praziquantel could be considered when no alternative drugs are available.

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Brief Communications
Identification of Antigenic Proteins in Trichomonas vaginalis
Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park
Korean J Parasitol 2011;49(1):79-83.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.79

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage λ Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, α-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.

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  • The Multifaceted Functions of Lactoferrin in Antimicrobial Defense and Inflammation
    Jung Won Kim, Ji Seok Lee, Yu Jung Choi, Chaekyun Kim
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    Emanuel Ceballos‐Góngora, Julio César Torres‐Romero, Victor Ermilo Arana‐Argáez, María Elizbeth Alvarez‐Sánchez, Karla Acosta‐Viana, Antonio Euan‐Canto, Leidi Cristal Alvarez‐Sánchez
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  • Trichomonas vaginalis adhesion protein 65 (TvAP65) modulates parasite pathogenicity by interacting with host cell proteins
    Zhenchao Zhang, Xiaoxiao Song, Yangyang Deng, Yuhua Li, Fakun Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Xuefang Mei, Shuai Wang
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    Hye-Yeon Lee, Juri Kim, Jae-Sook Ryu, Soon-Jung Park
    The Korean Journal of Parasitology.2017; 55(4): 375.     CrossRef
  • TvMP50 is an Immunogenic Metalloproteinase during Male Trichomoniasis
    Laura Itzel Quintas-Granados, José Luis Villalpando, Laura Isabel Vázquez-Carrillo, Rossana Arroyo, Guillermo Mendoza-Hernández, María Elizbeth Álvarez-Sánchez
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  • Epitopes of the Highly Immunogenic Trichomonas vaginalis α-Actinin Are Serodiagnostic Targets for Both Women and Men
    Calvin J. Neace, J. F. Alderete
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CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
Hyo-Jin Kim, Bong-Kwang Jung, Jin-Joo Lee, Kyoung-Ho Pyo, Tae Yun Kim, Byung-il Choi, Tae Woo Kim, Hajime Hisaeda, Kunisuke Himeno, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2011;49(1):85-90.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.85

Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

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    Vaccines.2022; 10(5): 762.     CrossRef
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    Yan Liang, Lei Cui, Li Xiao, Xiao Liu, Yourong Yang, Yanbo Ling, Tong Wang, Lan Wang, Jie Wang, Xueqiong Wu
    Frontiers in Immunology.2022;[Epub]     CrossRef
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    Infection and Immunity.2011; 79(9): 3642.     CrossRef
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Thelazia rhodesii in the African Buffalo, Syncerus caffer, in Zambia
Hetron Mweemba Munang'andu, Mweelwa Chembensofu, Victor M. Siamudaala, Musso Munyeme, Wigganson Matandiko
Korean J Parasitol 2011;49(1):91-94.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.91

We report 2 cases of Thelazia rhodesii infection in the African buffaloes, Syncerus caffer, in Zambia. African buffalo calves were captured from the livestock and wildlife interface area of the Kafue basin in the dry season of August 2005 for the purpose to translocate to game ranches. At capture, calves (n=48) were examined for the presence of eye infections by gently manipulating the orbital membranes to check for eye-worms in the conjunctival sacs and corneal surfaces. Two (4.3%) were infected and the mean infection burden per infected eye was 5.3 worms (n=3). The mean length of the worms was 16.4 mm (95% CI; 14.7-18.2 mm) and the diameter 0.41 mm (95% CI; 0.38-0.45 mm). The surface cuticle was made of transverse striations which gave the worms a characteristic serrated appearance. Although the calves showed signs of kerato-conjunctivitis, the major pathological change observed was corneal opacity. The calves were kept in quarantine and were examined thrice at 30 days interval. At each interval, they were treated with 200 ?g/kg ivermectin, and then translocated to game ranches. Given that the disease has been reported in cattle and Kafue lechwe (Kobus lechwe kafuensis) in the area, there is a need for a comprehensive study which aims at determining the disease dynamics and transmission patterns of thelaziasis between wildlife and livestock in the Kafue basin.

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  • Thelazia species (eyeworms) infection in cattle: Prevalence, species diversity, seasonal dynamics and its ocular effects in South Gondar zone, Northwest Ethiopia
    Tewodros Alemneh, Shimelis Dagnachew
    Veterinary Parasitology: Regional Studies and Reports.2025; 61: 101254.     CrossRef
  • Arthropod-Borne Zoonotic Parasitic Diseases in Africa: Existing Burden, Diversity, and the Risk of Re-Emergence
    Ayman Ahmed, Emmanuel Edwar Siddig, Nouh Saad Mohamed
    Parasitologia.2025; 5(3): 29.     CrossRef
  • Diversity of Thelazia spp. in domestic cattle from Romania: epidemiology and molecular diagnosis by a novel multiplex PCR
    Vlad-Dan Cotuțiu, Angela Monica Ionică, Teodora Dan, Cristina Daniela Cazan, Silvia Diana Borșan, Carla Andreea Culda, Marian Mihaiu, Călin Mircea Gherman, Andrei Daniel Mihalca
    Parasites & Vectors.2023;[Epub]     CrossRef
  • Dose-dependent effect of romifidine on intraocular pressure in clinically healthy buffalo (Bubalus bubalis)
    A. Rizk, I. Nocera, A. Briganti, K. Abouelnasr, S. El-Khodery, M. Tagawa, F. Bonelli
    Heliyon.2019; 5(12): e02930.     CrossRef
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Risk Factors Associated with Head Louse Infestation in Korea
Seobo Sim, Won-Ja Lee, Jae-Ran Yu, In Yong Lee, Seung Hyun Lee, Soo-Youn Oh, Min Seo, Jong-Yil Chai
Korean J Parasitol 2011;49(1):95-98.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.95

Head louse infestation (HLI) is one of the most frequently occurring parasitic diseases in children. This study was conducted to investigate the socioeconomic and personal factors influencing HLI in the Republic of Korea. A total of 2,210 questionnaires about various factors related to HLI were obtained from children in 17 primary schools throughout the country. The rate of HLI was significantly lower in children who lived together with mother or in a family where both parents worked. In addition, HLI was lower in children whose fathers or mothers were public officers or teachers. However, HLI was higher in children who had small families and washed their hair less often. Education levels of parents and the number of children in family were not significant. Improvement of socioeconomic factors and personal hygiene will be helpful for reducing HLI.

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