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"Kyung-il Im"

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"Kyung-il Im"

Original Article

Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage
Kyu-Lee Han, Jongweon Lee, Don-Soo Kim, Soon-Jung Park, Kyung-il Im, Tai-Soon Yong
Korean J Parasitol 2006;44(1):15-20.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.15

Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.

Citations

Citations to this article as recorded by  Crossref logo
  • Acanthamoeba culbertsoni: Electron‐Dense Granules in a Highly Virulent Clinical Isolate
    Bibiana Chávez‐Munguía, Lizbeth Salazar‐Villatoro, Maritza Omaña‐Molina, Martha Espinosa‐Cantellano, Elizabeth Ramírez‐Flores, Jacob Lorenzo‐Morales, Adolfo Martínez‐Palomo
    Journal of Eukaryotic Microbiology.2016; 63(6): 744.     CrossRef
  • Identification of a second mimicry epitope from Acanthamoeba castellanii that induces CNS autoimmunity by generating cross-reactive T cells for MBP 89-101 in SJL mice
    C. Massilamany, O. A. Asojo, A. Gangaplara, D. Steffen, J. Reddy
    International Immunology.2011; 23(12): 729.     CrossRef
  • Acanthamoeba healyi: Expressed gene profiles with enhanced virulence after mouse-brain passage
    Ying-Hua Xuan, Yeon-Chul Hong, Yong-Seok Lee, Se-Won Kang, Hak-Sun Yu, Tae-In Ahn, Dong-Il Chung, Hyun-Hee Kong
    Experimental Parasitology.2009; 123(3): 226.     CrossRef
  • 8,241 View
  • 77 Download
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Brief Communication

Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica
Seobo Sim, Kyeong Ah Kim, Tai-Soon Yong, Soon-Jung Park, Kyung-il Im, Myeong Heon Shin
Korean J Parasitol 2004;42(4):205-208.
Published online December 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.4.205

Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

Citations

Citations to this article as recorded by  Crossref logo
  • Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica
    Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
    The Korean Journal of Parasitology.2022; 60(3): 155.     CrossRef
  • The state of art of neutrophil extracellular traps in protozoan and helminthic infections
    César Díaz-Godínez, Julio C. Carrero
    Bioscience Reports.2019;[Epub]     CrossRef
  • Entamoeba histolytica Trophozoites Induce a Rapid Non-classical NETosis Mechanism Independent of NOX2-Derived Reactive Oxygen Species and PAD4 Activity
    César Díaz-Godínez, Zayda Fonseca, Mario Néquiz, Juan P. Laclette, Carlos Rosales, Julio C. Carrero
    Frontiers in Cellular and Infection Microbiology.2018;[Epub]     CrossRef
  • Entamoeba histolytica induces human neutrophils to form NETs
    J. Ventura‐Juarez, MR. Campos‐Esparza, J. Pacheco‐Yepez, J. A López‐Blanco, A. Adabache‐Ortíz, M. Silva‐Briano, R. Campos‐Rodríguez
    Parasite Immunology.2016; 38(8): 503.     CrossRef
  • Modulatory mechanisms of enterocyte apoptosis by viral, bacterial and parasitic pathogens
    Andre G Buret, Amol Bhargava
    Critical Reviews in Microbiology.2014; 40(1): 1.     CrossRef
  • Anoikis potential of Entameba histolytica secretory cysteine proteases: Evidence of contact independent host cell death
    Sudeep Kumar, Rajdeep Banerjee, Nilay Nandi, Abul Hasan Sardar, Pradeep Das
    Microbial Pathogenesis.2012; 52(1): 69.     CrossRef
  • In vivo programmed cell death of Entamoeba histolytica trophozoites in a hamster model of amoebic liver abscess
    J. D. Villalba-Magdaleno, G. Perez-Ishiwara, J. Serrano-Luna, V. Tsutsumi, M. Shibayama
    Microbiology.2011; 157(5): 1489.     CrossRef
  • TGF‐β‐regulated tyrosine phosphatases induce lymphocyte apoptosis in Leishmania donovani‐infected hamsters
    Rajdeep Banerjee, Sudeep Kumar, Abhik Sen, Ananda Mookerjee, Syamal Roy, Subrata Pal, Pradeep Das
    Immunology & Cell Biology.2011; 89(3): 466.     CrossRef
  • Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica
    Seobo Sim, Jae-Ran Yu, Young Ah Lee, Myeong Heon Shin
    The Korean Journal of Parasitology.2010; 48(4): 285.     CrossRef
  • Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica
    Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
    International Journal for Parasitology.2007; 37(11): 1209.     CrossRef
  • Involvement of β2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica
    Seobo Sim, Soon-Jung Park, Tai-Soon Yong, Kyung-Il Im, Myeong Heon Shin
    Microbes and Infection.2007; 9(11): 1368.     CrossRef
  • Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase‐3 and reducing Mcl‐1 expression
    J. H. KANG, H. O. SONG, J. S. RYU, M. H. SHIN, J. M. KIM, Y. S. CHO, J. F. ALDERETE, M. H. AHN, D. Y. MIN
    Parasite Immunology.2006; 28(9): 439.     CrossRef
  • Toxoplasma gondii Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation
    Ji-Young Kim, Myoung-Hee Ahn, Hye-Sun Jun, Jai-Won Jung, Jae-Sook Ryu, Duk-Young Min
    Yonsei Medical Journal.2006; 47(6): 862.     CrossRef
  • 7,984 View
  • 90 Download
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Mini Review

Pathogenic free-living amoebae in Korea
Ho-Joon Shin, Kyung-il Im
Korean J Parasitol 2004;42(3):93-119.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.93

Acanthamoeba and Naegleria are widely distributed in fresh water, soil and dust throughout the world, and cause meningoencephalitis or keratoconjunctivitis in humans and other mammals. Korean isolates, namely, Naegleria sp. YM-1 and Acanthamoeba sp. YM-2, YM-3, YM-4, YM-5, YM-6 and YM-7, were collected from sewage, water puddles, a storage reservoir, the gills of a fresh water fish, and by corneal washing. These isolates were categorized into three groups based on the mortalities of infected mice namely, highly virulent (YM-4), moderately virulent (YM-2, YM-5 and YM-7) and nonpathogenic (YM-3). In addition, a new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Korean isolate YM-4. The morphologic characters of its cysts were similar to those of A. culbertsoni and A. royreba, which were previously designated as Acanthamoeba group III. Based on experimentally infected mouse mortality, Acanthamoeba YM-4 was highly virulent. The isoenzymes profile of Acanthamoeba YM-4 was similar to that of A. royreba. Moreover, an anti-Acanthamoeba YM-4 monoclonal antibody reacted only with Acanthamoeba YM-4, and not with A. culbertsoni. Random amplified polymorphic DNA marker analysis and RFLP analysis of mitochondrial DNA and of a 18S small subunit ribosomal RNA, placed Acanthamoeba YM-4 in a separate cluster based on phylogenic distances. Thus Acanthamoeba YM-4 was identified as a new species, and assigned Acanthamoeba sohi. Up to the year 2002 in Korea, two clinical cases were found to be infected with Acanthamoeba spp. These patients died of meningoencephalitis. In addition, one case of Acanthamoeba pneumonia with an immunodeficient status was reported and Acanthamoeba was detected in several cases of chronic relapsing corneal ulcer, chronic conjunctivitis, and keratitis.

Citations

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  • Modelling dynamics between free‐living amoebae and bacteria
    Marwa Ali, Christopher A. Rice, Andrew W. Byrne, Philip E. Paré, Wendy Beauvais
    Environmental Microbiology.2024;[Epub]     CrossRef
  • Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1)
    Jong-Hyun Kim, Hae-Jin Sohn, Ho-Joon Shin, Stacy E. Walz, Suk-Yul Jung
    Parasites, Hosts and Diseases.2024; 62(4): 385.     CrossRef
  • Acanthamoeba spp. in river water samples from the Black Sea region, Turkey
    İlknur Koyun, Zeynep Kolören, Ülkü Karaman, Amalia Tsiami, Panagiotis Karanis
    Journal of Water and Health.2020; 18(2): 186.     CrossRef
  • “Proposals for Amendments in the Diagnosis and Treatment of Encephalitis caused by Free-living Amoebae”
    Abdul Mannan Baig
    Infectious Disorders - Drug Targets .2020; 20(2): 115.     CrossRef
  • Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran
    Mohammad Hossein Golestani, Sima Rasti, Hossein Hooshyar, Mahdi Delavari, Seyed Gholam Abbas Mousavi, Leila Iranshahi, Ali Aghajani
    Jundishapur Journal of Microbiology.2018;[Epub]     CrossRef
  • Biofilme mit Amöben, Bakterien und Pilzen im gebauten Umfeld des Menschen
    Wolfgang Karl Hofbauer
    Bauphysik.2018; 40(5): 396.     CrossRef
  • Production and characterization of monoclonal antibodies against cathepsin B and cathepsin B-Like proteins of Naegleria fowleri
    Gi-Sang Seong, Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Ho-Joon Shin
    Experimental Parasitology.2017; 183: 171.     CrossRef
  • The Acanthamoeba spp. in Water Sources from Zanjan Province, Northwest of Iran
    Ali Pezeshki, Elnaz Kadkhodamohammadi, Abbas Mahmmodzadeh, Ali Haniloo
    Journal of Human, Environment, and Health Promotion.2017; 2(3): 168.     CrossRef
  • Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran
    Ali Reza Latifi, Maryam Niyyati, Jacob Lorenzo-Morales, Ali Haghighi, Seyyed Javad Seyyed Tabaei, Zohreh Lasjerdi, Eznolah Azargashb
    Acta Parasitologica.2017;[Epub]     CrossRef
  • Soil Contamination With Free-Living Amoeba in North of Iran
    Mohammad Ali Mohaghegh, Mojtaba Azimi Resketi, Reza Mohammadimanesh, Mehdi Azami, Farzaneh Mirzaie, Mohammad Falahati, Somayeh Jahani, Mohsen Ghomashlooyan
    International Journal of Infection.2016;[Epub]     CrossRef
  • Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran
    Mohammad Reza Mahmoudi, Behnaz Rahmati, Seyed Hosssen Seyedpour, Panagiotis Karanis
    Parasitology Research.2015; 114(12): 4669.     CrossRef
  • Isolation and molecular identification of Naegleria fowleri from Nile river, Egypt
    Ahmad Z. Al-Herrawy, Mahmoud A. Gad
    Journal of the Egyptian Public Health Association.2015; 90(4): 161.     CrossRef
  • Coexistence of Legionella pneumophila Bacteria and Free-Living Amoebae in Lakes Serving as a Cooling System of a Power Plant
    Elżbieta Żbikowska, Hanna Kletkiewicz, Maciej Walczak, Aleksandra Burkowska
    Water, Air, & Soil Pollution.2014;[Epub]     CrossRef
  • Genotyping of Acanthamoeba Isolated From Surface and Stagnant Waters of Qazvin, Central Iran
    Hossein Hooshyar, Bahram Hosseinbigi, Mehrzad Saraei, Safarali Alizadeh, Mohammad Eftakhar, Sima Rasti, Nader Khosro-Shahi
    Iranian Red Crescent Medical Journal.2013; 15(6): 536.     CrossRef
  • Microbial contamination of contact lens storage cases and domestic tap water of contact lens wearers
    Miray Üstüntürk, Zuhal Zeybek
    Wiener klinische Wochenschrift.2012; 124(S3): 17.     CrossRef
  • Naegleria fowleriLysate Induces Strong Cytopathic Effects and Pro-inflammatory Cytokine Release in Rat Microglial Cells
    Yang-Jin Lee, Chang-Eun Park, Jong-Hyun Kim, Hae-Jin Sohn, Jinyoung Lee, Suk-Yul Jung, Ho-Joon Shin
    The Korean Journal of Parasitology.2011; 49(3): 285.     CrossRef
  • Activity of chlorpromazine on nfa1 and Mp2CL5 genes of Naegleria fowleri trophozoites
    Supathra Tiewcharoen, Jundee Rabablert, Prucksawan Chetannachan, Dusit Worawirunwong, Virach Junnu, Noppamas Pungsub
    Health.2011; 03(03): 166.     CrossRef
  • Thenfa1Gene Contributed on the Contact-dependent Pathogenic Mechanisms ofNaegleria fowleri
    Ho-Joon Shin
    Hanyang Medical Reviews.2010; 30(3): 204.     CrossRef
  • Changing Patterns of Human Parasitic Infection in Korea
    Myoung-Hee Ahn
    Hanyang Medical Reviews.2010; 30(3): 149.     CrossRef
  • The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri
    Hae-Jin Sohn, Jong-Hyun Kim, Myeong-Heon Shin, Kyoung-Ju Song, Ho-Joon Shin
    Parasitology Research.2010; 106(4): 917.     CrossRef
  • In vitro Susceptibility of Naegleria fowleri Trophozoites to Amphotericin B-combined Chlorpromazine
    S. Tiewcharoe, J. Rabablert, V. Junnu
    Research Journal of Microbiology.2009; 4(9): 320.     CrossRef
  • Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity
    Kyoung-Ju Song, Kyung-Hui Song, Jong-Hyun Kim, Hae-Jin Sohn, Yang-Jin Lee, Chang-Eun Park, Ho-Joon Shin
    Parasitology Research.2008; 103(2): 313.     CrossRef
  • Contact-Independent Cell Death of Human Microglial Cells due to Pathogenic Naegleria fowleri Trophozoites
    Jong-Hyun Kim, Daesik Kim, Ho-Joon Shin
    The Korean Journal of Parasitology.2008; 46(4): 217.     CrossRef
  • Recent Trend of Information Technology
    Seiichi Shin
    JAPAN TAPPI JOURNAL.2008; 62(3): 255.     CrossRef
  • Protozoa in respiratory pathology: a review
    R. Martínez-Girón, J. G. Esteban, A. Ribas, L. Doganci
    European Respiratory Journal.2008; 32(5): 1354.     CrossRef
  • Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri
    Kyoung-Ju Song, Kyung-Hui Song, Byoung-Kuk Na, Jong-Hyun Kim, Daeho Kwon, Sun Park, Jhang-Ho Pak, Kyung-Il Im, Ho-Joon Shin
    Parasitology Research.2007; 100(5): 1083.     CrossRef
  • Naegleria fowleri: Functional expression of the Nfa1 protein in transfected Naegleria gruberi by promoter modification
    Kyoung-Ju Song, Seok-Ryoul Jeong, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-Il Im, Jhang Ho Pak, Ho-Joon Shin
    Experimental Parasitology.2006; 112(2): 115.     CrossRef
  • Cytopathic changes and pro‐inflammatory cytokines induced by Naegleria fowleri trophozoites in rat microglial cells and protective effects of an anti‐Nfa1 antibody
    Y.‐H. OH, S.‐R. JEONG, J.‐H. KIM, K.‐J. SONG, K. KIM, S. PARK, S. SOHN, H.‐J. SHIN
    Parasite Immunology.2005; 27(12): 453.     CrossRef
  • Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Cocultured with CHO Target Cells
    Su-Yeon Kang, Kyoung-Ju Song, Seok-Ryoul Jeong, Jong-Hyun Kim, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Ho-Joon Shin
    Clinical and Vaccine Immunology.2005; 12(7): 873.     CrossRef
  • Expression of the nfa1 Gene Cloned from Pathogenic Naegleria fowleri in Nonpathogenic N. gruberi Enhances Cytotoxicity against CHO Target Cells In Vitro
    Seok-Ryoul Jeong, Sang-Chul Lee, Kyoung-Ju Song, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-il Im, Ho-Joon Shin
    Infection and Immunity.2005; 73(7): 4098.     CrossRef
  • 14,831 View
  • 99 Download
  • Crossref

Original Articles

Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri
Seok-Ryoul Jeong, Su-Yeon Kang, Sang-Chul Lee, Kyoung-Ju Song, Kyung-il Im, Ho-Joon Shin
Korean J Parasitol 2004;42(1):35-40.
Published online March 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.1.35

The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia-specific localization by immunocytochemistry in the previous study. Based on the idea that the pseudopodia-specific Nfa1 protein mentioned above seems to be involved in the pathogenicity of N. fowleri, we observed the effect of an anti-Nfa1 antibody on the proliferation of N. fowleri trophozoites and the cytotoxicity of N. fowleri trophozoites on the target cells. The proliferation of N. fowleri trophozoites was inhibited after being treated with an anti-Nfa1 polyclonal antibody in a dose-dependent manner for 48 hrs. By a light microscope, CHO cells co-cultured with N. fowleri trophozoites (group I) for 48 hrs showed severe morphological destruction. On the contrary, CHO cells co-cultured with N. fowleri trophozoites and anti-Nfa1 polyclonal antibody (1:100 dilution) (group II) showed less destruction. In the LDH release assay results, group I showed 50.6% cytotoxicity, and group II showed 39.3%. Consequently, addition of an anti-Nfa1 polyclonal antibody produced a decreasing effect of in vitro cytotoxicity of N. fowleri in a dosedependent manner.

Citations

Citations to this article as recorded by  Crossref logo
  • A review of the mechanism, diagnosis, and treatment of Naegleria fowleri infection
    Ling Dai, Xin-Ru Guo, Xu-Rui Chen, Ming-Hao Ma, Zi-Han Liu, Juan Lai, Jun Lu, Ming Feng, Xi-Xia Liu, Sheng-Hui Yang
    Frontiers in Microbiology.2025;[Epub]     CrossRef
  • Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1)
    Jong-Hyun Kim, Hae-Jin Sohn, Ho-Joon Shin, Stacy E. Walz, Suk-Yul Jung
    Parasites, Hosts and Diseases.2024; 62(4): 385.     CrossRef
  • Role of cathepsin B of Naegleria fowleri during primary amebic meningoencephalitis
    Itzel Berenice Rodríguez-Mera, María Maricela Carrasco-Yépez, Ismael Vásquez-Moctezuma, José Correa-Basurto, Gema Ramírez- Salinas, Diego Arturo Castillo-Ramírez, Érika Rosales-Cruz, Saúl Rojas-Hernández
    Parasitology Research.2022; 121(11): 3287.     CrossRef
  • Opinion: Iron, Climate Change and the ‘Brain Eating Amoeba’ Naegleria fowleri
    Sutherland K. Maciver, Paul J. McLaughlin, David K. Apps, Jose Enrique Piñero, Jacob Lorenzo-Morales
    Protist.2021; 172(1): 125791.     CrossRef
  • Cellular characterization of actin gene concerned with contact‐dependent mechanisms in Naegleria fowleri
    Hae‐Jin Sohn, Kyoung‐Ju Song, Heekyoung Kang, A‐Jeong Ham, Jae‐Ho Lee, Yong‐Joon Chwae, Kyongmin Kim, Sun Park, Jong‐Hyun Kim, Ho‐Joon Shin
    Parasite Immunology.2019;[Epub]     CrossRef
  • Biology and pathogenesis of Naegleria fowleri
    Ruqaiyyah Siddiqui, Ibne Karim M. Ali, Jennifer R. Cope, Naveed Ahmed Khan
    Acta Tropica.2016; 164: 375.     CrossRef
  • Protective immunity against Naegleria fowleri infection on mice immunized with the rNfa1 protein using mucosal adjuvants
    Jinyoung Lee, Jong-Kyun Yoo, Hae-Jin Sohn, Hee-kyoung Kang, Daesik Kim, Ho-Joon Shin, Jong-Hyun Kim
    Parasitology Research.2015; 114(4): 1377.     CrossRef
  • Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory–secretory proteins and their biochemical properties
    Jinyoung Lee, Jong-Hyun Kim, Hae-Jin Sohn, Hee-Jong Yang, Byoung-Kuk Na, Yong-Joon Chwae, Sun Park, Kyongmin Kim, Ho-Joon Shin
    Parasitology Research.2014; 113(8): 2765.     CrossRef
  • Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri
    Denise C Zysset-Burri, Norbert Müller, Christian Beuret, Manfred Heller, Nadia Schürch, Bruno Gottstein, Matthias Wittwer
    BMC Genomics.2014;[Epub]     CrossRef
  • Vaccination with Lentiviral Vector Expressing thenfa1Gene Confers a Protective Immune Response to Mice Infected with Naegleria fowleri
    Jong-Hyun Kim, Hae-Jin Sohn, Jinyoung Lee, Hee-Jong Yang, Yong-Joon Chwae, Kyongmin Kim, Sun Park, Ho-Joon Shin
    Clinical and Vaccine Immunology.2013; 20(7): 1055.     CrossRef
  • The immune response induced by DNA vaccine expressing nfa1 gene against Naegleria fowleri
    Jong-Hyun Kim, Sang-Hee Lee, Hae-Jin Sohn, Jinyoung Lee, Yong-Joon Chwae, Sun Park, Kyongmin Kim, Ho-Joon Shin
    Parasitology Research.2012; 111(6): 2377.     CrossRef
  • Naegleria fowleriLysate Induces Strong Cytopathic Effects and Pro-inflammatory Cytokine Release in Rat Microglial Cells
    Yang-Jin Lee, Chang-Eun Park, Jong-Hyun Kim, Hae-Jin Sohn, Jinyoung Lee, Suk-Yul Jung, Ho-Joon Shin
    The Korean Journal of Parasitology.2011; 49(3): 285.     CrossRef
  • Effects of immunization with the rNfa1 protein on experimental Naegleria fowleri‐PAM mice
    Y. J. LEE, J. H. KIM, H. J. SOHN, J. LEE, S. Y. JUNG, Y. J. CHWAE, K. KIM, S. PARK, H. J. SHIN
    Parasite Immunology.2011; 33(7): 382.     CrossRef
  • Thenfa1Gene Contributed on the Contact-dependent Pathogenic Mechanisms ofNaegleria fowleri
    Ho-Joon Shin
    Hanyang Medical Reviews.2010; 30(3): 204.     CrossRef
  • Gene silencing of nfa1 affects the in vitro cytotoxicity of Naegleria fowleri in murine macrophages
    Suk-Yul Jung, Jong-Hyun Kim, Kyoung-Ju Song, Yang-Jin Lee, Myung-Hee Kwon, Kyongmin Kim, Sun Park, Kyung-il Im, Ho-Joon Shin
    Molecular and Biochemical Parasitology.2009; 165(1): 87.     CrossRef
  • Immunodominant antigens in Naegleria fowleri excretory–secretory proteins were potential pathogenic factors
    Jong-Hyun Kim, Ae-Hee Yang, Hae-Jin Sohn, Daesik Kim, Kyoung-Ju Song, Ho-Joon Shin
    Parasitology Research.2009; 105(6): 1675.     CrossRef
  • Contact-Independent Cell Death of Human Microglial Cells due to Pathogenic Naegleria fowleri Trophozoites
    Jong-Hyun Kim, Daesik Kim, Ho-Joon Shin
    The Korean Journal of Parasitology.2008; 46(4): 217.     CrossRef
  • Naegleria fowleri: nfa1 gene knock-down by double-stranded RNAs
    Suk-Yul Jung, Jong-Hyun Kim, Yang-Jin Lee, Kyoung-Ju Song, Kyongmin Kim, Sun Park, Kyung-il Im, Ho-Joon Shin
    Experimental Parasitology.2008; 118(2): 208.     CrossRef
  • Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity
    Kyoung-Ju Song, Kyung-Hui Song, Jong-Hyun Kim, Hae-Jin Sohn, Yang-Jin Lee, Chang-Eun Park, Ho-Joon Shin
    Parasitology Research.2008; 103(2): 313.     CrossRef
  • Therapeutic effect of rokitamycin in vitro and on experimental meningoencephalitis due to Naegleria fowleri
    Jong-Hyun Kim, Yang-Jin Lee, Hae-Jin Sohn, Kyoung-Ju Song, Daeho Kwon, Myung-Hee Kwon, Kyung-Il Im, Ho-Joon Shin
    International Journal of Antimicrobial Agents.2008; 32(5): 411.     CrossRef
  • Effect of Therapeutic Chemical Agents In Vitro and on Experimental Meningoencephalitis Due toNaegleria fowleri
    Jong-Hyun Kim, Suk-Yul Jung, Yang-Jin Lee, Kyoung-Ju Song, Daeho Kwon, Kyongmin Kim, Sun Park, Kyung-Il Im, Ho-Joon Shin
    Antimicrobial Agents and Chemotherapy.2008; 52(11): 4010.     CrossRef
  • Production of Nfa1-specific monoclonal antibodies that influences the in vitro cytotoxicity of Naegleria fowleri trophozoites on microglial cells
    Yang-Jin Lee, Jong-Hyun Kim, Seok-Ryoul Jeong, Kyoung-Ju Song, Kyongmin Kim, Sun Park, Moon-Sung Park, Ho-Joon Shin
    Parasitology Research.2007; 101(5): 1191.     CrossRef
  • Naegleria fowleri: Functional expression of the Nfa1 protein in transfected Naegleria gruberi by promoter modification
    Kyoung-Ju Song, Seok-Ryoul Jeong, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-Il Im, Jhang Ho Pak, Ho-Joon Shin
    Experimental Parasitology.2006; 112(2): 115.     CrossRef
  • Cytopathic changes and pro‐inflammatory cytokines induced by Naegleria fowleri trophozoites in rat microglial cells and protective effects of an anti‐Nfa1 antibody
    Y.‐H. OH, S.‐R. JEONG, J.‐H. KIM, K.‐J. SONG, K. KIM, S. PARK, S. SOHN, H.‐J. SHIN
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  • Expression of the nfa1 Gene Cloned from Pathogenic Naegleria fowleri in Nonpathogenic N. gruberi Enhances Cytotoxicity against CHO Target Cells In Vitro
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  • Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Cocultured with CHO Target Cells
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Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish
Kyung-il Im, Ho-Joon Shin
Korean J Parasitol 2003;41(4):181-188.
Published online December 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.4.181

A new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Acanthamoeba sp. YM-4 (Korean isolate YM-4). The trophozoites were 11.0-23.0 ?m in length and had hyaline filamentous projections. Cysts were similar to those of A. culbertsoni and A. royreba, which were previously designated as Acanthamoeba group III. Acanthamoeba YM-4 can survive at 40℃, and its generation time was 19.6 hr, which was longer than that of A. culbertsoni. In terms of the in vitro cytotoxicity of lysates, Acanthamoeba YM-4 was weaker than A. culbertsoni, but stronger than A. polyphaga. On the basis of the mortality of experimentally infected mice, Acanthamoeba YM-4 was found to be highly virulent. The isoenzymes profile of Acanthamoeba YM-4 was similar to that of A. royreba. An anti-Acanthamoeba YM-4 monoclonal antibody, McAY7, was found to react only with Acanthamoeba YM-4, and not with A. culbertsoni. Random amplified polymorphic DNA marker analysis and RFLP analysis of mitochondrial DNA and of 18S small subunit ribosomal RNA, placed Acanthamoeba YM-4 in a separate cluster on the basis of phylogenetic distances. Thus the Acanthamoeba Korean isolate YM-4 was identified as a new species, and assigned as Acanthamoeba sohi.

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    Clinical and Vaccine Immunology.2005; 12(7): 873.     CrossRef
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A survey on head lice infestation in Korea (2001) and the therapeutic efficacy of oral trimethoprim/sulfamethoxazole adding to lindane shampoo
Seobo Sim, In-Yong Lee, Kyu-Jae Lee, Jang-Hoon Seo, Kyung-Il Im, Myeong Heon Shin, Tai-Soon Yong
Korean J Parasitol 2003;41(1):57-61.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.57

Total of 7,495 children including 3,908 boys and 3,587 girls from a kindergarten and 15 primary schools were examined for head lice infestation (HLI). The overall prevalence of HLI in this study was found to be 5.8%. Head lice were much more commonly detected in girls than in boys with prevalence of 11.2% and 0.9%, respectively. Sixty-nine children with HLI were treated with 1% lindane shampoo alone (group 1), and 45 children with HLI were treated with 1% lindane shampoo and oral trimethoprim/sulfamethoxazole (group 2), and follow-up visits were conducted 2 and 4 weeks later. The children who still had HLI 2 weeks after the primary treatment were treated again. At the 2-week follow-up visit, the treatment success rates of groups 1 and 2 were 76.8% and 86.7%, respectively, and at the 4-week follow-up visit, the rates were 91.3% and 97.8%, respectively. No statistically significant synergistic effect was observed for the combination of a 1% lindane shampoo and oral trimethoprim/sulfamethoxazole.

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Phylogenetic relationship of ribosomal ITS2 and mitochondrial COI among diploid and triploid Paragonimus westermani isolates
Gab-Man Park, Kyung-Il Im, Tai-Soon Yong
Korean J Parasitol 2003;41(1):47-55.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.47

We compared patterns of intraspecific polymorphism of two markers with contrasting modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the lung fluke, diploid and triploid Paragonimus westermani from three geographical regions of Korea. The genetic distances between three populations of Korean diploid and triploid P. westermani showed no significant difference in the nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (mtCOI) and ribosomaal second internal transcribed spacer (ITS2) genes. A highly resolved strict-consensus tree was obtained that illustrated phylogenetically useful information of the ITS2 and mtCOI sequences from diploid and triploid P. westermani.

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Case Report

A case of breast sparganosis
Seobo Sim, Jai-Kyung You, In-Yong Lee, Kyung-Il Im, Tai-Soon Yong
Korean J Parasitol 2002;40(4):187-189.
Published online December 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.4.187

A 29-year-old Korean woman visited the Department of Surgery in MizMedi Hospital with a palpable itching mass on the right breast that had existed for the past 7 months. She had no history to eat either frogs or snakes, but had the history of drinking impure water. Sonography revealed a serpiginous hypoechoic tubular structure associated with partial fat necrosis in breast parenchymal layer and subcutaneous fat layer. It also revealed oval cystic lesions. At operation, an ivory white opaque ribbon-like worm that measured 16.5 cm in length and 0.5 cm in width was extracted. Anti-sparganum specific serum IgG level in the patient's serum (absorbance = 0.71), measured by ELISA, was found to be significantly higher than those of normal controls (cut off point = 0.21). Sonography and ELISA appear to be helpful to diagnose sparganosis. Breast sparganosis is rarely found throughout the world.

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Brief Communication

Status of intestinal parasites infection among primary school children in Kampongcham, Cambodia
Kyu-Jae Lee, Yong-Tae Bae, Dong-Heui Kim, Young-Kun Deung, Yong-Suk Ryang, Hun-Joo Kim, Kyung-Il Im, Tai-Soon Yong
Korean J Parasitol 2002;40(3):153-155.
Published online September 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.3.153

A survey was made to find the extent of intestinal parasite infection in Kampongcham, Cambodia in February 2002. A total of 251 fecal specimens were collected from Tonlebat primary school children and examined by formalin-ether sedimentation technique. The overall infection rate of intestinal parasite was 54.2% (males, 57.3%; females, 50.8%). The infection rate of intestinal helminths by the species were as follows: Ascaris lumbricoides 26.3%, Echinostoma sp. 15.6%, hookworm 6.4%, Opisthorchis sp. 4.0%, Rhabditis sp. 2.4%, and Trichuris trichiura 0.4%. The infection rate of intestinal protozoa were as follows: E. coli 7.6%, G. lamblia 3.2%, I. butschlii 3.2%, and E. histolytica 0.8%. More than two different kinds of parasites were found in 16.7% of the stool samples. All the children infected were treated with albendazole, praziquantel and metronidazole according to parasite species. The results showed that intestinal parasites are highly endemic in this area.

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Original Article

Chromosomes of the liver fluke, Clonorchis sinensis
Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong
Korean J Parasitol 2000;38(3):201-206.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.201

A karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, Clonorchis sinensis (Trematoda: Opisthorchiidae), collected from Korea and China. Chromosome preparations were made by means of air-drying method. The chromosome number was 2n=56 in both Korean and Chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. However, the karyotypes showed some differences between Korean and Chinese flukes. The karyotype of liver flukes from Korea consisted of three metacentric pairs, one meta-/submetacentric pair, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes. On the other hand, liver flukes from China consisted of two metacentric pairs, two meta-/submetacentric pairs, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes.

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Brief Communications

Enterobius vermicularis egg positive rate in a primary school in Chungchongnam-do (Province) in Korea
Kyu-Jae Lee, In-Yong Lee, Kyung-il Im
Korean J Parasitol 2000;38(3):177-178.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.177

The egg positive rate of Enterobius vermicularis was investigated among students of a primary school and a kindergarten located in the rural area of Tangjin-gun, Chungchongnam-do in December, 1998. Of the 189 examinees, 28 (14.8%) were found to be infected with E. vermicularis by the adhesive cellotape anal swab method. The infection rates ranged from 4.2% to 26.1% among school children, and the highest rate was observed in children attending kindergarten. Three months after treatment with albendazole, four (14.3%) out of 28 infected children still remained infected with E. vermicularis. Through this survey, we were able to determine that E. vermicularis infection is still prevalent among children in rural areas of Korea.

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In vitro cytotoxicity of Acanthamoeba spp. isolated from contact lens containers in Korea by crystal violet staining and LDH release assay
Ho-Joon Shin, Myung-Soo Cho, Suk-Yul Jung, Hyung-Il Kim, Kyung-il Im
Korean J Parasitol 2000;38(2):99-102.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.99

In order to observe the cytotoxicity of Acanthamoeba spp., which were isolated from contact lens containers as ethiological agents for the probable amoebic keratitis in Korea, the crystal violet staining method and LDH release assay were carried out. In the crystal violet staining method, among eight contact lens container isolates, isolate 3 (Acanthamoeba KA/LS5) showed 83.6% and 81.8% of cytotoxicity, and isolate 7 (Acanthamoeba KA/LS37) showed 28.2% and 25.1% of cytotoxicity, in 1 mg/ml and 0.5 mg/ml lysate treatments, respectively. Acanthamoeba culbertsoni and A. healyi showed 84.0% and 82.8% of cytotoxicity. Similar results were observed in A. castellanii and A. hatchetti which showed 83.6% and 75.5% of cytotoxicity. Acanthamoeba royreba and A. polyphaga showed 9.0% and 1.7% of cytotoxicity. In the LDH release assay, isolate 3 (20.4%) showed higher cytotoxicity than other isolates in 1 mg/ml lysate treatment. The results provide that at least isolate 3 has the cytotoxic effect against CHO cells and seems to be the pathogenic strain.

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    Emad El-Meliegy, Mohammad M. Farag, Abeer M. El-Kady, Mervat S. Mohamed, Heba K. Abdelhakim, Mona Moaness
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  • Acanthamoeba royreba: Morphological features and in vitro cytopathic effect
    Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
    Experimental Parasitology.2013; 133(4): 369.     CrossRef
  • Effects of co‐culture of amoebae with indoor microbes on their cytotoxic and proinflammatory potential
    Terhi Yli‐Pirilä, Kati Huttunen, Aino Nevalainen, Markku Seuri, Maija‐Riitta Hirvonen
    Environmental Toxicology.2007; 22(4): 357.     CrossRef
  • Pathogenic free-living amoebae in Korea
    Ho-Joon Shin, Kyung-il Im
    The Korean Journal of Parasitology.2004; 42(3): 93.     CrossRef
  • Acanthamoebaspp. as Agents of Disease in Humans
    Francine Marciano-Cabral, Guy Cabral
    Clinical Microbiology Reviews.2003; 16(2): 273.     CrossRef
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Isozyme electrophoresis patterns of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China
Gab-Man Park, Tai-Soon Yong, Kyung-il Im, Kyu-Je Lee
Korean J Parasitol 2000;38(1):45-48.
Published online March 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.1.45

An enzyme analysis of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China was conducted using a horizontal starch gel electrophoresis in order to elucidate their genetic relationships. A total of eight enzymes was employed from two different kinds of buffer systems. Two loci from each enzyme of aconitase and esterase (α-Na and β-Na); and only one locus each from six enzymes, glucose-6-phosphate dehydrogenase (G6PD), α-glycerophosphate dehydrogenase (GPD), 3-hydroxybutyrate dehydrogenase (HBDH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), and phosphoglucomutase (PGM) were detected. Most of loci in two populations of C. sinensis showed homozygous monomorphic banding patterns and one of them, GPD was specific as genetic markers between two different populations. However, esterase (α-Na), GPD, HBDH and PGI loci showed polymorphic banding patterns. Two populations of C. sinensis were more closely clustered within the range of genetic identity value of 0.998-1.0. In summarizing the above results, two populations of C. sinensis employed in this study showed mostly monomorphic enzyme protein banding patterns, and genetic differences specific between two populations.

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  • Opisthorchis viverrini: Implications of the systematics of first intermediate hosts, Bithynia snail species in Thailand and Lao PDR
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    Jiufeng Sun, Yan Huang, Huaiqiu Huang, Pei Liang, Xiaoyun Wang, Qiang Mao, Jingtao Men, Wenjun Chen, Chuanhuan Deng, Chenhui Zhou, Xiaoli Lv, Juanjuan Zhou, Fan Zhang, Ran Li, Yanli Tian, Huali Lei, Chi Liang, Xuchu Hu, Jin Xu, Xuerong Li, XinbingYu, Dav
    PLoS ONE.2013; 8(6): e67006.     CrossRef
  • Genetic variation and relationships of four species of medically important echinostomes (Trematoda: Echinostomatidae) in South-East Asia
    Weerachai Saijuntha, Paiboon Sithithaworn, Kunyarat Duenngai, Nadda Kiatsopit, Ross H. Andrews, Trevor N. Petney
    Infection, Genetics and Evolution.2011; 11(2): 375.     CrossRef
  • Spatial and temporal genetic variation of Echinostoma revolutum (Trematoda: Echinostomatidae) from Thailand and the Lao PDR
    Weerachai Saijuntha, Chairat Tantrawatpan, Paiboon Sithithaworn, Ross H. Andrews, Trevor N. Petney
    Acta Tropica.2011; 118(2): 105.     CrossRef
  • Genetic differentiation of Echinostoma revolutum and Hypodereaum conoideum from domestic ducks in Thailand by multilocus enzyme electrophoresis
    W. Saijuntha, P. Sithithaworn, R.H. Andrews
    Journal of Helminthology.2010; 84(2): 143.     CrossRef
  • Functional Genes and Proteins of Clonorchis sinensis
    Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
    The Korean Journal of Parasitology.2009; 47(Suppl): S59.     CrossRef
  • Genetic variation at three enzyme loci within a Thailand population of Opisthorchis viverrini
    Weerachai Saijuntha, Paiboon Sithithaworn, Sopit Wongkham, Thewarach Laha, Rojchai Satrawaha, Neil B. Chilton, Trevor N. Petney, Ross H. Andrews
    Parasitology Research.2008; 103(6): 1283.     CrossRef
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    De-Hua Lai, Qiao-Ping Wang, Wen Chen, Lian-Shun Cai, Zhong-Dao Wu, Xing-Quan Zhu, Zhao-Rong Lun
    Acta Tropica.2008; 107(2): 213.     CrossRef
  • Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences
    Gab-Man Park
    Parasitology Research.2007; 100(2): 351.     CrossRef
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    Weerachai Saijuntha, Paiboon Sithithaworn, Sopit Wongkham, Thewarach Laha, Vichit Pipitgool, Smarn Tesana, Neil B. Chilton, Trevor N. Petney, Ross H. Andrews
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  • Genetic markers for the identification and characterization of Opisthorchis viverrini, a medically important food borne trematode in Southeast Asia
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    Acta Tropica.2006; 100(3): 246.     CrossRef
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Original Articles

Upregulated expression of the cDNA fragment possibly related to the virulence of Acanthamoeba culbertsoni
Kyung-Il Im, Kwang-Min Park, Tai-Soon Yong, Yong-Pyo Hong, Tae-Eun Kim
Korean J Parasitol 1999;37(4):257-263.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.257

Identification of the genes responsible for the recovery of virulence in brain-passaged Acanthamoeba culbertsoni was attempted via mRNA differential display-polymerase chain reaction (mRNA DD-PCR) analysis. In order to identify the regulatory changes in transcription of the virulence related genes by the brain passages, mRNA DD-PCR was performed which enabled the display of differentially transcribed mRNAs after the brain passages. Through mRNA DD-PCR analysis, 96 brain-passaged amoeba specific amplicons were observed and were screened to identify the amplicons that failed to amplify in the non-brain-passaged amoeba mRNAs. Out of the 96 brain-passaged amoeba specific amplicons, 12 turned out to be amplified only from the brain-passaged amoeba mRNAs by DNA slot blot hybridization. The clone, A289C, amplified with an arbitrary primer of UBC #289 and the oligo dT11-C primer, revealed the highest homology (49.8%) to the amino acid sequences of UPD-galactose lipid transferase of Erwinia amylovora, which is known to act as an important virulence factor. The deduced amino acid sequences of an insert DNA in clone A289C were also revealed to be similar to cpsD, which is the essential gene for the expression of type III capsule in group B streptococcus. Upregulated expression of clone A289C was verified by RNA slot blot hybridization. Similar hydrophobicity values were also observed between A289C (at residues 47-66) and the AmsG gene of E. amylovora (at residues 286-305: transmembrane domains). This result suggested that the insert of clone A289C might play the same function as galactosyl transferase controlled by the AmsG gene in E. amylovora.

Citations

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  • Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms of Acanthamoeba castellanii in the Ex Vivo Amoebic Keratitis Model
    Lizbeth Salazar-Villatoro, Bibiana Chávez-Munguía, Celia Esther Guevara-Estrada, Anel Lagunes-Guillén, Dolores Hernández-Martínez, Ismael Castelan-Ramírez, Maritza Omaña-Molina
    Pathogens.2023; 12(8): 1049.     CrossRef
  • Acanthamoebaspp. as Agents of Disease in Humans
    Francine Marciano-Cabral, Guy Cabral
    Clinical Microbiology Reviews.2003; 16(2): 273.     CrossRef
  • 7,226 View
  • 49 Download
  • Crossref
Isoenzyme patterns and phylogenetic relationships in Acanthamoeba spp. isolated from contact lens containers in Korea
Ho-Joon Shin, Myung-Soo Cho, Han-Jip Kim, Kyung-Il Im
Korean J Parasitol 1999;37(4):229-236.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.229

In order to refer to the basic information regarding the identification of isolates obtained from a contact lens container in Korea, the isoelectric focusing gel electrophoresis was employed to compare the isoenzyme band patterns among Acanthamoeba spp. including eight isolates and the simple pairwise dissimilarity analysis was carried out. For an alkaline phosphate development, isolate 7 and Acanthamoeba polyphaga showed homologous band patterns, and isolates 1, 2, and 3 showed the same patterns. For lactate dehydrogenase, similar patterns were observed in isolates 2 and 3. Isolates 3 and 5 showed homologous band patterns for malate dehydrogenase and glucose phosphate isomerase. For hexokinase, isolates 4, 7, and A. hatchetti showed the same band patterns. In others, a considerable number of interstrain polymorphisms was observed in nine isoenzyme band patterns. In Acanthamoeba group II, genetic distances among isolates 1, 2, 3, 4, and 5 ranged from 0.104 to 0.200. In comparison to A. castellanii, A. hatchetti, and A. polyphaga, genetic distances of isolates 7 and 8 were 0.254 and 0.219, respectively. In Acanthamoeba group III, including A. culbertsoni, A. healyi, and A. royreba, isolate 6 had genetic distances which ranged from 0.314 to 0.336. Finally, when comparing to the six reference Acanthamoeba, it was possible to classify isolates 1, 2, 3, 4, and 5, as genetically close-related species and as independent species group. Furthermore, isolates 6, 7 and 8 were identified as independent species as well.

Citations

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  • Diagnosis of Acanthamoeba keratitis
    N. R. Marchenko, Evg. A. Kasparova
    Vestnik oftal'mologii.2016; 132(5): 103.     CrossRef
  • Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case
    Hak Sun Yu, Hae Jin Jeong, Yeon-Chul Hong, Seong-Yong Seol, Dong-Il Chung, Hyun-Hee Kong
    The Korean Journal of Parasitology.2007; 45(1): 11.     CrossRef
  • Pathogenic free-living amoebae in Korea
    Ho-Joon Shin, Kyung-il Im
    The Korean Journal of Parasitology.2004; 42(3): 93.     CrossRef
  • 9,442 View
  • 62 Download
  • Crossref
Pathogenicity of Korean isolates of Acanthamoeba by observing the experimental infection and zymodemes of five isoenzymes
Kyung-Il Im, Ho-Joon Shin, Dong Whan Seo, Soung-Hoo Jeon, Tae-Eun Kim
Korean J Parasitol 1999;37(2):85-92.
Published online June 30, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.2.85

To determine the pathogenicity of Acanthamoeba spp. isolated in Korea and to develop a isoenzymatic maker, the mortality rate of infected mice, in vitro cytotoxicity against target cells and isoenzyme band patterns were observed. Five isolates of Acanthamoeba spp. (YM-2, YM-3, YM-4, YM-5, and YM-7) were used in this study as well as three reference Acanthamoeba spp. (A. culbertsoni, A. hatchetti, and A. royreba). According to the mortality rate of infected mice, Korean isolates could be categorized into three groups: high virulent (YM-4), low virulent (YM-2, YM-5, YM-7) and the nonpathogenic group (YM-3). In addition, the virulence of Acanthamoeba spp. was enhanced by brain passage in mice. In the cytotoxicity assay against chinese hamster ovary cells, especially, the cytotoxicity of brain-passaged amoebae was relatively higher than the long-term cultivated ones. The zymodeme patterns of glucose-6-phosphate dehydrogenase (G6PD), malate dehydrogenase (MDH), hexokinase (HK), glutamate oxaloacetate transaminase (GOT) and malic enzyme (ME) of Acanthamoeba spp. were different among each isolate, and also between long-term cultured amoebae and brain passaged ones. In spites of the polymorphic zymodemes, a slow band of G6PD and HK, and an intermediate band of MDH were only observed in pathogenic Acanthamoeba spp., which should be used as isoenzymatic makers.

Citations

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  • Experimental infection of T4 Acanthamoeba genotype determines the pathogenic potential
    Daniella de Sousa Mendes Moreira Alves, Aline Silva Moraes, Luciano Moreira Alves, Rodrigo Gurgel-Gonçalves, Ruy de Souza Lino Junior, César Augusto Cuba-Cuba, Marina Clare Vinaud
    Parasitology Research.2016; 115(9): 3435.     CrossRef
  • Acanthamoeba royreba: Morphological features and in vitro cytopathic effect
    Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
    Experimental Parasitology.2013; 133(4): 369.     CrossRef
  • Pathogenic free-living amoebae in Korea
    Ho-Joon Shin, Kyung-il Im
    The Korean Journal of Parasitology.2004; 42(3): 93.     CrossRef
  • Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish
    Kyung-il Im, Ho-Joon Shin
    The Korean Journal of Parasitology.2003; 41(4): 181.     CrossRef
  • Cytopathic Changes in Rat Microglial Cells Induced by PathogenicAcanthamoeba culbertsoni: Morphology and Cytokine Release
    Ho-Joon Shin, Myung-Soo Cho, Suk-Yul Jung, Hyung-Il Kim, Sun Park, Jang-Hoon Seo, Jung-Chil Yoo, Kyung-Il Im
    Clinical Diagnostic Laboratory Immunology.2001; 8(4): 837.     CrossRef
  • In vitro cytotoxicity of Acanthamoeba spp. isolated from contact lens containers in Korea by crystal violet staining and LDH release assay
    Ho-Joon Shin, Myung-Soo Cho, Suk-Yul Jung, Hyung-Il Kim, Kyung-il Im
    The Korean Journal of Parasitology.2000; 38(2): 99.     CrossRef
  • Apoptosis of Primary-Culture Rat Microglial Cells Induced by PathogenicAcanthamoebaspp
    Ho-Joon Shin, Myung-Soo Cho, Hyung-Il Kim, Millina Lee, Sun Park, Seonghyang Sohn, Kyung-Il Im
    Clinical Diagnostic Laboratory Immunology.2000; 7(3): 510.     CrossRef
  • 9,203 View
  • 74 Download
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Brief Communication
Characterization of YS-27, an axenic Korean strain of Entamoeba histolytica
Soon-Jung Park, Tai-Soon Yong, Jae-Kyung Chang, Kyung-Il Im, Chin-Thack Soh
Korean J Parasitol 1999;37(1):59-62.
Published online March 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.59

Characterization of YS-27, an axenic Entamoeba strain, was performed by three different laboratory methods. Zymodeme analysis using starch gel electrophoresis and PCR with species-specific primers showed that YS-27 is a pathogenic Entamoeba which belongs to the group II zymodeme. Pathogenicity of YS-27 was further confirmed by observing the formation of liver abscess in Mongolian gerbils. These results showed that YS-27 is E. hisolytica.

  • 7,730 View
  • 54 Download