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Brief Communication

Molecular detection of Borrelia theileri in cattle in Korea
Hyeon-Ji Hyung, Yun-Sil Choi, Jinho Park, Kwang-Jun Lee, Jun-Gu Kang
Parasites Hosts Dis 2024;62(1):151-156.
Published online February 23, 2024
DOI: https://doi.org/10.3347/PHD.23105
Bovine borreliosis, caused by Borrelia theileri which is transmitted via hard tick bites, is associated with mild clinical symptoms, such as fever, lethargy, hemoglobinuria, anorexia, and anemia. Borrelia theileri infects various animals, such as cattle, deer, horses, goats, sheep, and wild ruminants, in Africa, Australia, and South America. Notably, no case of B. theileri infection has been reported in Korean cattle to date. In this study, 101 blood samples were collected from a Korean indigenous cattle breed, among which 1.98% tested positive for B. theileri via nested PCR. The obtained sequences exhibited high homology with B. theileri strains identified in other regions. Phylogenetic analysis of 16S rRNA confirmed the B. theileri group affiliation; however, flagellin B sequences exhibited divergence, potentially due to regional evolutionary differences. This study provides the first molecular confirmation of B. theileri infection in Korean livestock. Further isolation and nucleotide sequence analyses are necessary to better understand the presence of B. theileri strains in cows in Korea.

Citations

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  • Nationwide Geographical and Temporal Distribution of Tick-Borne Diseases in Korean Water Deer (Hydropotes inermis argyropus)
    Beoul Kim, Su-Jin Chae, You-Jeong Lee, Haksub Shin, Sunmin Kwak, Hyesung Jeong, Suwoong Lee, Dongmi Kwak, Min-Goo Seo
    Animals.2025; 15(10): 1499.     CrossRef
  • Circulation of tick-borne pathogens in wildlife of the Republic of Korea
    Hye-ryung Byun, Seong-Ryeong Ji, Jun-Gu Kang, Chang-Yong Choi, Ki-Jeong Na, Jong-Taek Kim, Joon-Seok Chae
    One Health.2024; 19: 100913.     CrossRef
  • 3,754 View
  • 82 Download
  • 2 Web of Science
  • Crossref

Original Article

Latitude and seasons influence the prevalence of Theileria orientalis and affect the hematology of non-grazed dairy cows in Korea
Hector Espiritu, Hee-Woon Lee, Md Shohel Al Faruk, Su-Jeong Jin, Sang-Suk Lee, Yong-Il Cho
Parasites Hosts Dis 2024;62(1):64-74.
Published online February 23, 2024
DOI: https://doi.org/10.3347/PHD.23087
This pilot study aimed to investigate the effects of regional and seasonal variations on the prevalence of Theileria orientalis and the hematological profile of non-grazed dairy cows in Korea. A total of 365 clinically healthy lactating Holstein Friesian cows from 26 dairy farms in 7 provinces that were categorized into northern, central, and southern regions were sampled during the warm period from July to August and the cold period from October to December. The detection of T. orientalis major piroplasm surface protein gene and the hematology non-grazed dairy cows were analyzed using peripheral blood samples. The T. orientalis prevalence was 20.0% (73/365). The prevalence in the southern region was 35.9%, which was significantly higher than that in the central (21.6%) and northern (12.9%) regions (P < 0.05). The prevalence during warm period was higher (43.0%) than that during the cold season (13.5%). The infected cows showed significantly lower erythrocyte counts in the southern region (5.8 ± 0.6 M/µl) and during the warm period (5.8 ± 0.7 M/µl) compared with those in the central and northern regions and during the cold season, which affected the extended RBC parameters, including hematocrit and hemoglobin concentrations. Our findings revealed the prevalence of T. orientalis in Korea, highlighting its high occurrence during warm periods and in certain geographical regions. Climatic factors could contribute to the health and productivity of cattle, as evidenced by the prevalence of T. orientalis and its negative impact on animals.

Citations

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  • Characterization and identification of novel polymorphisms in the OR51H1 gene associated with resistance/tolerance to natural Theileria infection in Vechur (Bos indicus) and crossbred (B. taurus × B. indicus) cattle in a Theileria-endemic region
    K. Kalaiyarasi, Muhammed Elayadeth-Meethal
    Veterinary Parasitology: Regional Studies and Reports.2025; 60: 101242.     CrossRef
  • Molecular characterization of tick genera Haemaphysalis, Ixodes, and Amblyomma in Korea
    Subin Lee, Badriah Alkathiri, Kyu-Sung Ahn, Jiwon Kim, So Youn Youn, Mi-Sun Yoo, Hyang-Sim Lee, Jae-Myung Kim, Dongmi Kwak, Sung Shik Shin, Seung-Hun Lee
    Parasites, Hosts and Diseases.2025; 63(3): 272.     CrossRef
  • 3,824 View
  • 83 Download
  • 2 Web of Science
  • Crossref

Brief Communication

Molecular epidemiology of Toxoplasma gondii in cattle in Korea
Kyoo-Tae Kim, Min-Goo Seo
Parasites Hosts Dis 2023;61(2):210-215.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.23016
Toxoplasmosis is a major public health concern, with raw or undercooked meat being the primary source of human infection. Knowledge regarding the molecular epidemiology of Toxoplasma gondii in cattle destined for human consumption in Korea is lacking. The present study aimed to genetically characterize the infectious strains of T. gondii. Overall, 455 cattle blood samples from 84 farms in the Gyeongnam-do (Province) were randomly collected in 2017. Nested PCR analysis revealed that only 3 (0.7%) samples were infected with T. gondii. The B1 gene sequence of T. gondii was observed to be similar (97.3–99.6%) to that of other T. gondii isolates. This is the first study to perform the molecular detection of T. gondii in cattle in Korea. Although the prevalence of infection was low, our findings suggest that cattle present a potential public health issue. It may be crucial to recognize the importance of T. gondii infection in cattle meat.

Citations

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  • Molecular detection of Toxoplasma gondii in ticks and their respective host dogs
    Min-Goo Seo, Dongmi Kwak
    Parasites, Hosts and Diseases.2025; 63(1): 66.     CrossRef
  • Prevalence and molecular characterization of intestinal parasites in shelter dogs from South Korea
    You-Jeong Lee, Beoul Kim, Gyeonghyeon Lee, Yong-Myung Kang, Dongmi Kwak, Min-Goo Seo
    Research in Veterinary Science.2025; 188: 105620.     CrossRef
  • Antileishmanial and Antitoxoplasmal Activities of 1,4-Dihydropyridines
    Thaís A. S. Oliveira, Yan R. Robles, Ibrahim S. Al Nasr, Waleed S. Koko, Tariq A. Khan, Ismail Daoud, Seyfeddine Rahali, Noureddine Amdouni, Ridha B. Said, Antônio E. M. Crotti
    ACS Omega.2025; 10(28): 31066.     CrossRef
  • Tick-borne pathogens in Mongolian ticks: The high prevalence of Rickettsia raoultii and its public health implications
    You-Jeong Lee, Beoul Kim, Dongmi Kwak, Ochirkhuu Nyamsuren, Nyamdavaa Guugandaa, Min-Goo Seo
    Acta Tropica.2024; 260: 107412.     CrossRef
  • 4,154 View
  • 165 Download
  • 4 Web of Science
  • Crossref

Original Articles

Molecular epidemiological survey of pathogenic trypanosomes in naturally infected cattle in northern Côte d’ivoire
Jean-Yves Ekra, Edouard K. N’Goran, Leonard E.G. Mboera, Biégo Guillaume Gragnon, Koco Rita Nadège Assovié, Eliakunda Michael Mafie
Parasites Hosts Dis 2023;61(2):127-137.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.22170
Bovine trypanosomiasis is a significant health concern for livestock intensification in Côte d’Ivoire. This study aimed to determine the prevalence and distribution of pathogenic trypanosomes and identify the most infected cattle breed in northern Côte d'Ivoire. We examined 700 cattle and found that polymerase chain reaction (PCR) was more sensitive (12.3%) than microscopic observation (5.6%). Among the trypanosome species detected in naturally infected cattle, Trypanosoma vivax was 7.3%, Trypanosoma simiae tsavo was 6.7%, and Trypanosoma congolense was 0.4%. The overall prevalence of trypanosome infection in all cattle breeds was 12.3%, while the prevalence in individual breeds was 14.8%, 7.3%, 10.6%, and 12.3% for N’Dama, Baoule, Zebu, and Mere breed, respectively. The infected animals had low packed cell volume, influencing the prevalence. Our findings indicate that bovine trypanosomes are prevalent in Côte d’Ivoire, and their prevalence varies by region and breed. These pathogens include T. vivax, T. simiae tsavo, and T. congolense.

Citations

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  • Prediction of the molecular action of Trypanosoma vivax on bovine reproductive parameters and risk factors associated with trypanosomiasis in northern Minas Gerais, Brazil
    Amanda Cristielly Nunes De Lima, Joely Ferreira Figueiredo Bittar, Otaviano de Souza Pires Neto, Eliane Macedo Sobrinho Santos, Priscilla Elias Ferreira da Silva, Hércules Otacílio Santos, Cintya Neves de Souza, Franciane Gabrielle dos Santos, Anna Christ
    Veterinary World.2025; : 837.     CrossRef
  • Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH
    Jean-Yves Ekra, Eliakunda Michael Mafie, Edouard K. N’Goran, Dramane Kaba, Biégo Guillaume Gragnon, Jagan Srinivasan
    Pathogens.2024; 13(3): 262.     CrossRef
  • Trypanocide Use and Molecular Characterization of Trypanosomes Resistant to Diminazene Aceturate in Cattle in Northern Côte D’Ivoire
    Jean-Yves Ekra, Eliakunda Michael Mafie, Henri Sonan, Michael Kanh, Biégo Guillaume Gragnon, Edouard K. N’Goran, Jagan Srinivasan
    Tropical Medicine and Infectious Disease.2024; 9(9): 192.     CrossRef
  • 6,642 View
  • 209 Download
  • 3 Web of Science
  • Crossref
Genotypes of Echinococcus Species from Cattle in Tanzania
Mohammed Mebarek Bia, Seongjun Choe, Barakaeli Abdieli Ndosi, Hansol Park, Yeseul Kang, Chatanun Eamudomkarn, Tilak Chandra Nath, Sunmin Kim, Hyeong-Kyu Jeon, Dongmin Lee, Keeseon S. Eom
Korean J Parasitol 2021;59(5):457-464.
Published online October 22, 2021
DOI: https://doi.org/10.3347/kjp.2021.59.5.457
Cystic echinococcosis is a zoonotic parasitic disease caused by Echinococcus species. Tanzania is one of the endemic countries with cystic echinococcosis. This study focussed on identifying genotypes of Echinococcus spp. in Tanzania. We collected 7 cysts from cattle in Mwanza municipal (n=4) and Loliondo district (n=3). The cysts from Mwanza were all E. ortleppi and fertile. In contrast, the cysts from Loliondo were all E. granulosus sensu stricto and sterile. Two from the 4 cysts were a new haplotype of E. ortleppi (G5). These results can improve the preventive and control programs for humans and livestock in Tanzania. To our knowledge, this study is considered the first to identify the genotype and haplotype of Echinococcus spp. in Tanzania.

Citations

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  • Occurrence of Echinococcus felidis in Apex Predators and Warthogs in Tanzania: First Molecular Evidence of Leopards as a New, Definitive Host and Implications for Ecosystem Health
    Barakaeli Abdieli Ndossi, Eblate Ernest Mjingo, Mary Wokusima Zebedayo, Seongjun Choe, Hansol Park, Lee Dongmin, Keeseon S. Eom, Mohammed Mebarek Bia
    Pathogens.2025; 14(5): 443.     CrossRef
  • 5,615 View
  • 124 Download
  • 2 Web of Science
  • Crossref

Brief Communications

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified
Sen Li, Yang Zou, Xue-Liang Zhang, Ping Wang, Xiao-Qing Chen, Xing-Quan Zhu
Korean J Parasitol 2020;58(6):681-687.
Published online December 29, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.6.681
Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The
objective
s of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

Citations

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  • Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals
    Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan
    PLOS Neglected Tropical Diseases.2025; 19(4): e0013021.     CrossRef
  • Investigation of Infection of Enterocytozoon bieneusi and Giardia duodenalis in Beef Cattle in Yunnan, China
    Fan Yang, Wenjie Cheng, Jianfa Yang, Junjun He, Liujia Li, Fengcai Zou, Fanfan Shu
    Veterinary Sciences.2025; 12(6): 552.     CrossRef
  • An Epidemiology Study on Distribution of Giardia lamblia in Incheon City
    Su Jin Park, Ju Hee Kim, Hyeon-Jeong Go, Nam Yee Kim, Sung Suck Oh, Young Woo Gong, MunJu Kwon, Jeong-An Gim, Soo-Son Lim
    Biomedical Science Letters.2025; 31(2): 218.     CrossRef
  • Genetic Characterization and Zoonotic Potential of Cryptosporidium spp. and Giardia duodenalis in Cattle From Northeast China
    Qile Yu, Sining Chen, Xichen Zhang, Qi Zhao, Mengfei Xu, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Nan Zhang, Xiangrui Li
    Transboundary and Emerging Diseases.2025;[Epub]     CrossRef
  • Molecular Epidemiology of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Guizhou Angus Calves: Dominance of Angus Cattle-Adapted Genotypes and Zoonotic Potential of E. bieneusi
    Peixi Qin, Zhuolin Tao, Kaizhi Shi, Jiaxian Zhao, Bingyan Huang, Hui Liu, Chunqun Wang, Jigang Yin, Guan Zhu, Simone M. Cacciò, Min Hu
    Microorganisms.2025; 13(8): 1735.     CrossRef
  • First report of Giardia duodenalis in dairy cattle and beef cattle in Shanxi, China
    Li Zhao, Yan Wang, Mingyuan Wang, Shan Zhang, Lifeng Wang, Zhansheng Zhang, Hailiang Chai, Chao Yi, Wenjun Fan, Yonghong Liu
    Molecular Biology Reports.2024;[Epub]     CrossRef
  • Prevalence and molecular characterization of Giardia duodenalis in dairy cattle in Central Inner Mongolia, Northern China
    Li Zhao, Zhan-Sheng Zhang, Wen-Xiong Han, Bo Yang, Hai-Liang Chai, Ming-Yuan Wang, Yan Wang, Shan Zhang, Wei-Hong Zhao, Yi-Min Ma, Yong-Jie Zhan, Li-Feng Wang, Yu-Lin Ding, Jin-Ling Wang, Yong-Hong Liu
    Scientific Reports.2023;[Epub]     CrossRef
  • Meta-Analysis of the Prevalence of Giardia duodenalis in Cattle in China
    Xiang-Zhu Meng, Chao Kang, Jiaqi Wei, He Ma, Gang Liu, Jin-Ping Zhao, Hong-Shun Zhang, Xin-Bo Yang, Xiang-Yu Wang, Li-Hua Yang, Hong-Li Geng, Hongwei Cao
    Foodborne Pathogens and Disease.2023; 20(1): 17.     CrossRef
  • Giardia duodenalis in Hu sheep: occurrence and environmental contamination on large-scale housing farms
    Qianming Zhao, Chenyang Lu, Zhiyang Pei, Pihong Gong, Junqiang Li, Fuchun Jian, Bo Jing, Meng Qi, Changshen Ning
    Parasite.2023; 30: 2.     CrossRef
  • Prevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Diarrheic and Non-Diarrheic Calves from Ningxia, Northwestern China
    Haihui Gao, Gaoxing Liang, Na Su, Qirui Li, Dong Wang, Jiandong Wang, Long Zhao, Xiaodong Kang, Kangkang Guo
    Animals.2023; 13(12): 1983.     CrossRef
  • Microbial Risks Caused by Livestock Excrement: Current Research Status and Prospects
    Rashidin Abdugheni, Li Li, Zhen-Ni Yang, Yin Huang, Bao-Zhu Fang, Vyacheslav Shurigin, Osama Abdalla Abdelshafy Mohamad, Yong-Hong Liu, Wen-Jun Li
    Microorganisms.2023; 11(8): 1897.     CrossRef
  • Multilocus Genotyping of Giardia duodenalis Occurring in Korean Native Calves
    Sang-Ik Oh, Suk-Han Jung, Han-Kyoung Lee, Changyong Choe, Tai-Young Hur, Kyoung-Min So
    Veterinary Sciences.2021; 8(7): 118.     CrossRef
  • 5,358 View
  • 76 Download
  • 12 Web of Science
  • Crossref
Molecular Detection of Coxiella burnetii in Cattle on Ulleung Island, Korea: A Population-based Study with Four Years of Follow Up
Min-Goo Seo, Oh-Deog Kwon, Dongmi Kwak
Korean J Parasitol 2019;57(1):69-73.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.69
In a population-based study with 4 years of follow up, we evaluated the prevalence of Coxiella burnetii in cattle on Ulleung Island, Korea. In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3-1.0% in the period 2011-2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide sequences of the 2 samples showed high (98.4-100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the transmission of C. burnetii on Ulleung Island, control strategy should include biosecurity improvement in surveillance, livestock management, administering suitable tests before purchasing animals to detect C. burnetii shedders, and restricting movements between herds.

Citations

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  • Comprehensive Analysis of SPL Genes in Cucurbitaceae Species: Evolutionary Insights, Functional Diversity and Regulatory Mechanisms
    PavanKalyan Neelam, Balakishore Lalam, Jaswanth Manavi, Ghanishtha Prusty, Suman Polaki, Pushpalatha Ganesh
    Iranian Journal of Science.2025; 49(5): 1209.     CrossRef
  • 6,272 View
  • 128 Download
  • 1 Web of Science
  • Crossref
Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
Nguyen Thi Hong Chien, Thi Lan Nguyen, Khanh Linh Bui, Tho Van Nguyen, Thanh Hoa Le
Korean J Parasitol 2019;57(1):43-47.
Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.43
Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Vietnam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular detection and genetic diversity of Anaplasma and Ehrlichia species in livestock and dogs in Pakistan
    Mughees Aizaz Alvi, Talha Javaid, Abu Bakar Yameen, Fakiha Asghar, Aliza Ali, Iqra Zaheer, Yousef Abdal Jalil Fadladdin, Hong-Bin Yan, Wan-Zhong Jia, Muhammad Saqib, Li Li
    Acta Tropica.2025; 268: 107700.     CrossRef
  • Isolation, Molecular Characterization and In Vitro Propagation of an Anaplasma platys-Like Bacterium in Tick Cells
    Erica Rodrigues de Matos, Priscilla Nunes dos Santos, Erich Peter Zweygarth, Talys Henrique Assumpção Jardim, Huarrisson Azevedo Santos, Matheus Dias Cordeiro, Bruna de Azevedo Baêta, Lesley Bell-Sakyi, Adivaldo Henrique da Fonseca, Claudia Bezerra da Sil
    Pathogens.2025; 14(9): 901.     CrossRef
  • Tick distribution and comparative analysis of bovine blood microbiome in two provinces of South Africa using 16S rRNA PacBio sequencing approach
    Bongekile L. Khoza, Charles Byaruhanga, S. Marcus Makgabo, Nkululeko Nyangiwe, Themba Mnisi, Samukelo Nxumalo, Marinda C. Oosthuizen, Zamantungwa T. H. Mnisi
    Frontiers in Tropical Diseases.2024;[Epub]     CrossRef
  • Molecular Characterization of Anaplasma spp. in Cattle from Kazakhstan
    Madina Kadyrova, Alexandr Ostrovskii, Kassym Mukanov, Amirkhan Kassen, Elena Shevtsova, Maxat Berdikulov, Gilles Vergnaud, Alexandr Shevtsov
    Pathogens.2024; 13(10): 894.     CrossRef
  • Detection of Tick‐Borne Pathogen Coinfections and Coexposures to Foot‐and‐Mouth Disease, Brucellosis, and Q Fever in Selected Wildlife From Kruger National Park, South Africa, and Etosha National Park, Namibia
    Carlo Andrea Cossu, Sunday Ochonu Ochai, Milana Troskie, Axel Hartmann, Jacques Godfroid, Lin-Mari de Klerk, Wendy Turner, Pauline Kamath, Ockert Louis van Schalkwyk, Rudi Cassini, Raksha Bhoora, Henriette van Heerden, Nan-Hua Chen
    Transboundary and Emerging Diseases.2024;[Epub]     CrossRef
  • Dynamics of Infections in Cattle and Rhipicephalus microplus: A Preliminary Study
    Elianne Piloto-Sardiñas, Angélique Foucault-Simonin, Alejandra Wu-Chuang, Lourdes Mateos-Hernández, Roxana Marrero-Perera, Lianet Abuin-Denis, Lisset Roblejo-Arias, Cristian Díaz-Corona, Zbigniew Zając, Joanna Kulisz, Aneta Woźniak, Sara Moutailler, Belki
    Pathogens.2023; 12(8): 998.     CrossRef
  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys in cattle in Nigeria
    Joshua Kamani, Juliane Schaer, Ali G. Umar, James Y. Pilarshimwi, Laminu Bukar, Javier González-Miguel, Shimon Harrus
    Ticks and Tick-borne Diseases.2022; 13(4): 101955.     CrossRef
  • Genetic diversity of Anaplasma marginale in calves with anaplasmosis on farms in Minas Gerais, Brazil
    Michele Bahia, Lucas Teixeira da Silva, Bruno Montijo da Silva, Matheus dias Cordeiro, Alexandro Guterres, Claudia Bezerra da Silva, Jenevaldo Barbosa da Silva, Adivaldo Henrique Fonseca
    Ticks and Tick-borne Diseases.2021; 12(1): 101552.     CrossRef
  • Comparison of Anaplasma and Ehrlichia species–specific peptide ELISAs with whole organism–based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs
    Barbara A. Qurollo, Brett A. Stillman, Melissa J. Beall, Paulette Foster, Barbara C. Hegarty, Edward B. Breitschwerdt, Ramaswamy Chandrashekar
    American Journal of Veterinary Research.2021; 82(1): 71.     CrossRef
  • Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia
    Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto
    Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001.     CrossRef
  • Molecular detection of selected tick-borne pathogens infecting cattle at the wildlife–livestock interface of Queen Elizabeth National Park in Kasese District, Uganda
    Benedicto Byamukama, Patrick Vudriko, Maria Agnes Tumwebaze, Dickson Stuart Tayebwa, Joseph Byaruhanga, Martin Kamilo Angwe, Jixu Li, Eloiza May Galon, Aaron Ringo, Mingming Liu, Yongchang Li, Shengwei Ji, Mohamed Abdo Rizk, Paul Frank Adjou Moumouni, Seu
    Ticks and Tick-borne Diseases.2021; 12(5): 101772.     CrossRef
  • Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle
    Onyinyechukwu Ada Agina, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Mazlina Mazlan, Azim Salahuddin Muhamad, Afrah Alhana Kassim, Lee Chai Ha, Fairuz Hazwani Rusli, Darulmuqaamah Masaud, Hazilawati Hamzah
    BMC Veterinary Research.2021;[Epub]     CrossRef
  • Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam
    Ly Na Huynh, Adama Zan Diarra, Quang Luan Pham, Nhiem Le-Viet, Jean-Michel Berenger, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola, Lynn Soong
    PLOS Neglected Tropical Diseases.2021; 15(9): e0009813.     CrossRef
  • The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal
    Marcos Rogério André, Ana Cláudia Calchi, Heitor Miraglia Herrera, Diego Carlos de Souza Zanatto, Bruna de Carvalho Le Senéchal Horta, Júlia Banhareli Tasso, Inalda Angélica de Souza Ramos, Victória Valente Califre de Mello, Rosangela Zacarias Machado
    Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100437.     CrossRef
  • Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand
    Anh H. L. Nguyen, Sonthaya Tiawsirisup, Morakot Kaewthamasorn
    BMC Veterinary Research.2020;[Epub]     CrossRef
  • 8,843 View
  • 132 Download
  • 17 Web of Science
  • Crossref

Original Article

Analysis of Five Arboviruses and Culicoides Distribution on Cattle Farms in Jeollabuk-do, Korea
Daram Yang, Myeon-Sik Yang, Haerin Rhim, Jae-Ik Han, Jae-Ku Oem, Yeon-Hee Kim, Kyoung-Ki Lee, Chae-Woong Lim, Bumseok Kim
Korean J Parasitol 2018;56(5):477-485.
Published online October 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.5.477
Arthropod-borne viruses (Arboviruses) are transmitted by arthropods such as Culicoides biting midges and cause abortion, stillbirth, and congenital malformation in ruminants, apparently leading to economic losses to farmers. To monitor the distribution of Culicoides and to determine their relationship with different environmental conditions (temperature, humidity, wind speed, and altitude of the farms) on 5 cattle farms, Culicoides were collected during summer season (May-September) in 2016 and 2017, and analyzed for identification of species and detection of arboviruses. About 35% of the Culicoides were collected in July and the collection rate increased with increase in temperature and humidity. The higher altitude where the farms were located, the more Culicoides were collected on inside than outside. In antigen test of Culicoides against 5 arboviruses, only Chuzan virus (CHUV) (2.63%) was detected in 2016. The Akabane virus (AKAV), CHUV, Ibaraki virus and Bovine ephemeral fever virus (BEFV) had a positive rate of less than 1.8% in 2017. In antigen test of bovine whole blood, AKAV (12.96%) and BEFV (0.96%) were positive in only one of the farms. As a result of serum neutralization test, antibodies against AKAV were generally measured in all the farms. These results suggest that vaccination before the season in which the Culicoides are active is probably best to prevent arbovirus infections.

Citations

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    Miaomiao Zhang, Menghua Deng, Sisi Zhao, Dengshuai Zhao, Yajie Zheng, Limei Qin, Han Gao, Mengmeng Zhao, Keshan Zhang
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • The Role of Hematophagous Arthropods, Other than Mosquitoes and Ticks, in Arbovirus Transmission
    Bradley J. Blitvich
    Viruses.2025; 17(7): 932.     CrossRef
  • Coinfection of Bovine ephemeral fever virus and ‘Candidatus Mycoplasma haemobos’ in cattle in Central China: implications for tick-borne transmission and viral divergence
    Hongfei Shi, Tingting Lv, Zhengtian Zhang, Hongyue Zhai, Li Wang, Shidong Ji, Dandan Li, Lunguang Yao
    BMC Veterinary Research.2025;[Epub]     CrossRef
  • Hiding in plain sight: Uncovering the hidden diversity of Culicoides spp. (Diptera: Ceratopogonidae) in the Republic of Korea using DNA barcoding data
    Jiseung Jeon, Dong-Yeol Lee, Seung Bak An, Jihun Ryu, Jong-Uk Jeong, In-Soon Roh, Kwang Shik Choi
    Acta Tropica.2025; 270: 107821.     CrossRef
  • Bovine ephemeral fever: From underestimated illness to emerging threat - A review of pathogenesis, economic impact, and future control strategies
    Shruti Pyasi, Neha Rani Sahu, Umasish Mohanty, Debasis Nayak
    Research in Veterinary Science.2025; 196: 105904.     CrossRef
  • Research progress on the N gene of Akabane virus
    Ruining Wang, Fang Liang, Xiaolin Lan, Gan Li, Feng Li, Mengmeng Zhao
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Potential roles of Culicoides spp. (Culicoides imicola, Culicoides oxystoma) as biological vectors of bluetongue virus in Yuanyang of Yunnan, P. R. China
    Nan Li, Jinxin Meng, Yuwen He, Wenhua Wang, Jinglin Wang
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
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    Jung‐Yong Yeh, Yun Ji Ga
    Veterinary Medicine and Science.2023; 9(1): 301.     CrossRef
  • Composition and diversity of Culicoides biting midges (Diptera: Ceratopogonidae) in rural and suburban environments of Algeria
    Nadia Kadjoudj, Azzedine Bounamous, Yacine Kouba, Bilal Dik, Samir Zeroual, Aicha Amira, Haroun Chenchouni
    Acta Tropica.2022; 234: 106588.     CrossRef
  • Chuzan virus infection

    CABI Compendium.2022;[Epub]     CrossRef
  • Genetically modified rabies virus vector‐based bovine ephemeral fever virus vaccine induces protective immune responses against BEFV and RABV in mice
    Wenwen Zheng, Zhongxin Zhao, Li Tian, Lele Liu, Tong Xu, Xianwei Wang, Hongbin He, Xianzhu Xia, Ye Zheng, Yurong Wei, Xuexing Zheng
    Transboundary and Emerging Diseases.2021; 68(3): 1353.     CrossRef
  • Detection of arboviruses in Culicoides (Diptera: Ceratopogonidae) collected from animal farms in the border areas of Yunnan Province, China
    Di DI, Chen-xi LI, Zong-jie LI, Xin WANG, Qi-qi XIA, Mona SHARMA, Bei-bei LI, Ke LIU, Dong-hua SHAO, Ya-feng QIU, WAI Soe-Soe, Shi-biao YANG, Jian-chao WEI, Zhi-yong MA
    Journal of Integrative Agriculture.2021; 20(9): 2491.     CrossRef
  • Attraction and Repellent Behaviors of Culicoides Biting Midges toward Cow Dung, Carbon Dioxide, and Essential Oils
    Daram Yang, Myeon-Sik Yang, Bumseok Kim
    The Korean Journal of Parasitology.2021; 59(5): 465.     CrossRef
  • The Ecology and Evolution of Japanese Encephalitis Virus
    Peter Mulvey, Veasna Duong, Sebastien Boyer, Graham Burgess, David T. Williams, Philippe Dussart, Paul F. Horwood
    Pathogens.2021; 10(12): 1534.     CrossRef
  • Investigation of bovine ephemeral fever virus transmission by putative dipteran vectors under experimental conditions
    Jessica E. Stokes, Karin E. Darpel, Simon Gubbins, Simon Carpenter, María del Mar Fernández de Marco, Luis M. Hernández-Triana, Anthony R. Fooks, Nicholas Johnson, Christopher Sanders
    Parasites & Vectors.2020;[Epub]     CrossRef
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    Fan Lee
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  • 16 Web of Science
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Brief Communication

Prevalence of Tick-Borne Pathogens from Ticks Collected from Cattle and Wild Animals in Tanzania in 2012
Tae Yun Kim, You Shine Kwak, Ju Yeong Kim, Sung-Hyun Nam, In-Yong Lee, Simon Mduma, Julius Keyyu, Robert Fyumagwa, Tai-Soon Yong
Korean J Parasitol 2018;56(3):305-308.
Published online June 30, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.3.305
This study was aimed to disclose the prevalence rate of tick-borne pathogens from ticks collected from cattle and wild animals in Tanzania in 2012. Ticks were collected from slaughtered cattle and dead wild animals from November 5 to December 23, 2012 and identified. PCR for detecting Anaplasmataceae, Piroplamidae, Rickettsiaceae, Borrelia spp., and Coxiella spp. were done. Among those tested, Rickettsiaceae, Piroplasmidae, and Anaplasmataceae, were detected in ticks from the 2 regions. Rickettsiaceae represented the major tick-borne pathogens of the 2 regions. Ticks from animals in Maswa were associated with a higher pathogen detection rate compared to that in ticks from Iringa. In addition, a higher pathogen detection rate was observed in ticks infesting cattle than in ticks infesting wild animals. All examined ticks of the genus Amblyomma were infected with diverse pathogens. Ticks of the genera Rhipicephalus and Hyalomma were infected with 1 or 2 pathogens. Collectively, this study provides important information regarding differences in pathogen status among various regions, hosts, and tick species in Tanzania. Results in this study will affect the programs to prevent tick-borne diseases (TBD) of humans and livestock in Tanzania.

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  • Molecular detection and prevalence of Anaplasma and Rickettsia species in rodents captured from wildlife-human interfaces in Iringa and Morogoro regions, Tanzania
    Venance T. Msoffe, Festo K. Mwombeki, Claus A. Thomas, Charles M. Lyimo, Abdul S. Katakweba, Moses I. Olotu, Gerald Misinzo, Erick V. Komba, Ladslaus L. Mnyone
    Mammalia.2025; 89(4): 437.     CrossRef
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    Komi Mensah Agboka, Allan Muohi Ngángá, Bonoukpoè Mawuko Sokame, Steve Soh Bernard Baleba, Tobias Landmann, Elfatih M. Abdel-Rahman, Chrysantus M. Tanga, Souleymane Diallo
    Spatial and Spatio-temporal Epidemiology.2025; 55: 100756.     CrossRef
  • Tick-Borne Diseases in Sub-Saharan Africa: A Systematic Review of Pathogens, Research Focus, and Implications for Public Health
    Tidjani A. Djiman, Abel S. Biguezoton, Claude Saegerman
    Pathogens.2024; 13(8): 697.     CrossRef
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    Nejib Doss, Aldo Morrone, Patrizia Forgione, Giusto Trevisan, Serena Bonin
    Biology.2024; 13(11): 897.     CrossRef
  • Prevalence and diversity of spotted fever group Rickettsia species in ixodid ticks from domestic dogs in Chad, Africa
    Stephanie Osip, Morgan Friedman, Ellen Haynes, Sarah M. Coker, John A. Bryan II, Metinou Sidouin, Philip Tchindebet Ouakou, Bongo Nare Richard Ngandolo, Christopher A. Cleveland, Michael J. Yabsley
    Ticks and Tick-borne Diseases.2024; 15(6): 102405.     CrossRef
  • Distribution and Prevalence of Anaplasmataceae, Rickettsiaceae and Coxiellaceae in African Ticks: A Systematic Review and Meta-Analysis
    Carlo Andrea Cossu, Nicola E. Collins, Marinda C. Oosthuizen, Maria Luisa Menandro, Raksha Vasantrai Bhoora, Ilse Vorster, Rudi Cassini, Hein Stoltsz, Melvyn Quan, Henriette van Heerden
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  • Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control
    Agatha Kolo
    Pathogens.2023; 12(5): 702.     CrossRef
  • Ticks and Tick-Borne Pathogens in Domestic Animals, Wild Pigs, and Off-Host Environmental Sampling in Guam, USA
    Genevieve V. Weaver, Neil Anderson, Kayla Garrett, Alec T. Thompson, Michael J. Yabsley
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Epidemiology of Ticks and Tick-Borne Pathogens in Domestic Ruminants across Southern African Development Community (SADC) Region from 1980 until 2021: A Systematic Review and Meta-Analysis
    Mpho Tawana, ThankGod E. Onyiche, Tsepo Ramatla, Sibusiso Mtshali, Oriel Thekisoe
    Pathogens.2022; 11(8): 929.     CrossRef
  • The diversity and evolutionary relationships of ticks and tick-borne bacteria collected in China
    JunHua Tian, Xin Hou, MiHong Ge, HongBin Xu, Bin Yu, Jing Liu, RenFu Shao, Edward C. Holmes, ChaoLiang Lei, Mang Shi
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    Shola David Ola-Fadunsin, Reuben Sunil Kumar Sharma, Donea Abdurazak Abdullah, Fufa Ido Gimba, Faez Firdaus Jesse Abdullah, Rehana Abdullah Sani
    Ticks and Tick-borne Diseases.2021; 12(3): 101653.     CrossRef
  • Prioritizing smallholder animal health needs in East Africa, West Africa, and South Asia using three approaches: Literature review, expert workshops, and practitioner surveys
    Zoë Campbell, Paul Coleman, Andrea Guest, Peetambar Kushwaha, Thembinkosi Ramuthivheli, Tom Osebe, Brian Perry, Jeremy Salt
    Preventive Veterinary Medicine.2021; 189: 105279.     CrossRef
  • Comparative microbiomes of ticks collected from a black rhino and its surrounding environment
    Seogwon Lee, Ju Yeong Kim, Myung-hee Yi, In-Yong Lee, Robert Fyumagwa, Tai-Soon Yong
    International Journal for Parasitology: Parasites and Wildlife.2019; 9: 239.     CrossRef
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Original Articles

First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal
Haroon Ahmed, S?rgio Ramalho Sousa, Sami Simsek, Sofia Anast?cio, Seyma Gunyakti Kilinc
Korean J Parasitol 2017;55(6):653-658.
Published online December 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.6.653
Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The
objective
of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Citations

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  • Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia
    Ema Gagović, Daria Jurković Žilić, Krunoslav Pintur, Adnan Hodžić, Šimun Naletilić, Relja Beck
    Animals.2025; 15(2): 208.     CrossRef
  • Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain)
    Sara González, Rosario Panadero, María Luisa Del Rio, María Natividad Díez, María del Rosario Hidalgo, Angélica Martínez
    Veterinary Research Communications.2024; 48(2): 761.     CrossRef
  • Molecular characterization of the camel nasal botfly, Cephalopina titillator (Diptera: Oestridae)
    Mona G. Shaalan, Sherif Hamed Farghaly, Emad I. Khater, Mohamed A. Kenawy, Enas Hamdy Ghallab
    Beni-Suef University Journal of Basic and Applied Sciences.2024;[Epub]     CrossRef
  • Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis
    Sara González, Maria Luisa Del Rio, Maria Natividad Diez, Maria del Rosario Hidalgo, Angelica Martínez
    Microscopy Research and Technique.2023; 86(1): 3.     CrossRef
  • Temporal and spatial spread of Hypoderma actaeon infection in roe deer from peninsular Spain determined by an indirect enzyme‐linked immunosorbent assay
    R. Panadero, C. M. López, S. Remesar, E. Cabanelas, G. Varas, F. Markina, P. Díaz, D. García‐Dios, A. Prieto, G. Fernández, P. Díez‐Baños, P. Morrondo
    Medical and Veterinary Entomology.2020; 34(1): 44.     CrossRef
  • 8,669 View
  • 155 Download
  • 8 Web of Science
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Hematological Changes Associated with Theileria orientalis Infection in Korean Indigenous Cattle
Suhee Kim, Do-Hyeon Yu, Sung-Woo Kang, Jeong-Byoung Chae, Kyoung-Seong Choi, Hyeon-Cheol Kim, Bae-Keun Park, Joon-Seok Chae, Jinho Park
Korean J Parasitol 2017;55(5):481-489.
Published online October 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.5.481
Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015. Cattle that grazed in mountainous areas showed a greater prevalence of tick-borne infections with an increased Theileria orientalis infection rate (54.7%) compared to that in non-grazing cattle (16.3%) (P<0.001). Accordingly, the red blood cell (RBC) count and hematocrit (HCT) values of grazing cattle were significantly lower than those of non-grazing cattle throughout the season (P<0.05). Moreover, RBC, hemoglobin (Hb), and HCT of T. orientalis-positive group were significantly lower than those of T. orientalis-negative group (P<0.05). T. orientalis is a widespread tick-borne pathogen in Korea. Grazing of cattle in mountainous areas is closely associated with an increase in T. orientalis infection (RR=3.4, P<0.001), and with consequent decreases in RBC count and HCT. Thus, these findings suggest that the Hanwoo cattle in mountainous areas of Korea are at a high risk of infection by T. orientalis, which can lead to hematological alterations. This study highlights the necessity of preventive strategies that target T. orientalis infection.

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  • Pathogen Profiling and Molecular Evaluation Along With In Silico Modeling of Voltage‐Gated Sodium Channel Gene in Deltamethrin Exposed Haemaphysalis longicornis Ticks
    Muhammad Kashif Obaid, Qiaoyun Ren, Xiaoqing Zan, Jin Luo, Jehan Zeb, Muhammad Rashid, Guangyuan Liu, Laikang Yang, Xiujun Yun, Lijun Ma, Peng Ma, Changchun Ma, Xiaomei Yang, Hong Yin, Guiquan Guan
    The FASEB Journal.2025;[Epub]     CrossRef
  • Evaluation of haematological parameters in haemolytic anaemia caused by tick‐borne pathogens in grazing cattle
    Youngjun Kim, Ji‐Young Ku, Youngwoo Jung, Young‐Hwan Lim, Min‐Jeong Ji, Yu‐Jin Park, Hyung‐Chul Cho, Kyoung‐Seong Choi, Jinho Park
    Veterinary Medicine and Science.2024;[Epub]     CrossRef
  • Identification of hemolytic anemia in Korean indigenous cattle with a criteria value of reticulocyte count, indirect bilirubin, and L-lactate concentration
    Youngwoo Jung, Ji-Yeong Ku, Youngjun Kim, WooChan Kim, Hyungjae So, Lianfu Piao, Ji-Seon Yoon, Jinho Park
    Frontiers in Veterinary Science.2024;[Epub]     CrossRef
  • Molecular epidemiology of Theileria species in ticks and its potential threat to livestock in the Republic of Korea
    Badriah Alkathiri, KyuSung Ahn, Haeseung Lee, Yun Sang Cho, So Youn Youn, Min-Goo Seo, Dongmi Kwak, SungShik Shin, Seung-Hun Lee
    Acta Tropica.2023; 238: 106780.     CrossRef
  • Emergence of Theileria species in ticks from free-ranging domestic animals in Raymond Mhlaba local municipality, South Africa
    Benson Chuks Iweriebor, Kayode Olayinka Afolabi, Ayabulela Nqoro, Larry Chikwelu Obi
    Heliyon.2022; 8(3): e09085.     CrossRef
  • The role of sheep (Ovis aries) in maintaining Theileria orientalis Ikeda type infection
    K.E. Lawrence, K. Gedye, R. Hickson, B. Wang, L. Carvalho, Y. Zhao, W.E. Pomroy
    Veterinary Parasitology.2021; 291: 109391.     CrossRef
  • A clinical case of bovine anemia due to Theileria orientalis group in a non-grazed dairy cow in the upper part of South Korea
    Hector M. Espiritu, Hee-Woon Lee, Sang-Suk Lee, Yong-Il Cho
    Korean Journal of Veterinary Research.2021; 61(4): e33.     CrossRef
  • A genetic and immunological comparison of tick-resistance in beef cattle following artificial infestation with Rhipicephalus ticks
    J. K. Marima, C. L. Nel, M. C. Marufu, N. N. Jonsson, B. Dube, K. Dzama
    Experimental and Applied Acarology.2020; 80(4): 569.     CrossRef
  • Genotypic Analysis of Piroplasms and Associated Pathogens from Ticks Infesting Cattle in Korea
    Min-Goo Seo, Oh-Deog Kwon, Dongmi Kwak
    Microorganisms.2020; 8(5): 728.     CrossRef
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    Onyinyechukwu Ada Agina, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Hazilawati Hamzah
    Pathogens.2020; 9(9): 697.     CrossRef
  • Genetic Diversity of Bovine Hemoprotozoa in South Korea
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    Pathogens.2020; 9(9): 768.     CrossRef
  • Correlation between hematological profile and theileriosis in Bali cattle from Muara Bulian, Jambi, Indonesia
    N. Aziz, M. Maksudi, Y. A. Prakoso
    Veterinary World.2019; 12(9): 1358.     CrossRef
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  • 183 Download
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Brief Communication

First Record of Paramphistomes Fischoederius cobboldi and Paramphistomum epiclitum Detected in Bovine Rumen from a Local Market of Savannakhet Province, Lao PDR
Surapol Sanguankiat, Marcello Otake Sato, Megumi Sato, Wanna Maipanich, Tippayarat Yoonuan, Tiengkham Pongvongsa, Boungnong Boupha, Yuichi Chigusa, Kazuhiko Moji, Jitra Waikagul
Korean J Parasitol 2016;54(4):543-547.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.543
In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.

Citations

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  • Exploring medicinal plants for antiparamphistomiasis: A scoping review of pharmacological insights and methodologies
    Iman Surya Pratama, Wisnu Nurcahyo, Enade Perdana Istyastono, Triana Hertiani
    Veterinary Parasitology.2025; 338: 110522.     CrossRef
  • Evolutionary affinities and morphological characterization of the enigmatic Zonocotyle bicaecata (Trematoda: Paramphistomoidea: Zonocotylidae) from the Upper Paraná River basin
    Letícia C. Bedin, Philippe V. Alves, Reinaldo J. da Silva
    Systematic Parasitology.2024;[Epub]     CrossRef
  • Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
    Nazir Ahmad Tookhy, Nur-Mahiza Md Isa, Yasmin Abd Rahaman, Nur Indah Ahmad, Reuben Sunil Kumar Sharma, Lokman Hakim Idris, Rozaihan Mansor, Dung Thi Bui, Noor Hazfalinda Hamzah, Shoaib Ahmad Shakhes
    Parasitology Research.2024;[Epub]     CrossRef
  • Differentiating paramphistome species in cattle using DNA barcoding coupled with high-resolution melting analysis (Bar-HRM)
    Kittisak Buddhachat, Sirikhwan Sriuan, Sirapat Nak-on, Thapana Chontananarth
    Parasitology Research.2023; 122(3): 769.     CrossRef
  • Rumen Fluke in Cattle and Buffaloes in Asia: A Review
    Nazir Ahmad Tookhy, Md Isa Nur-Mahiza, Rozaihan Mansor, Abd Rahman Yasmin, Nur Indah Ahmad, Noor Hazfalinda Hamzah, Lokman Hakim Idri
    Pertanika Journal of Tropical Agricultural Science.2022; 45(3): 781.     CrossRef
  • Prevalence of gastrointestinal helminths in ruminants in Ukraine: a 5-year meta-analysis
    O. V. Kruchynenko, S. M. Mykhailiutenko, M. A. Petrenko, L. M. Kuzmenko
    Biosystems Diversity.2021; 29(3): 251.     CrossRef
  • Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1
    Sothorn Anucherngchai, Thapana Chontananarth, Thanawan Tejangkura, Chalobol Wongsawad
    Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100382.     CrossRef
  • Rumen fluke, Fischoederius elongatus (Trematoda: Gastrothylacidae): Preliminary investigation of suitable conditions for egg hatching
    Sirapat Nak-on, Thapana Chontananarth
    Veterinary Parasitology.2020; 282: 109135.     CrossRef
  • Transmission of Leishmania infantum from cats to dogs
    Joilson Ferreira Batista, Francisco das Chagas Ribeiro Magalhães Neto, Kayo Sandro Pimentel do Prado Lopes, Marcello Otake Sato, Carlos Henrique Nery Costa, Ivete Lopes de Mendonça
    Revista Brasileira de Parasitologia Veterinária.2020;[Epub]     CrossRef
  • First genetic evidence for the presence of the rumen fluke Paramphistomum epiclitum in Pakistan
    Qasim Ali, Imran Rashid, Muhammad Zubair Shabbir, Haroon Akbar, Kashif Shahzad, Kamran Ashraf, Neil Sargison, Umer Chaudhry
    Parasitology International.2018; 67(5): 533.     CrossRef
  • Preference of A/T ending codons in mitochondrial ATP6 gene under phylum Platyhelminthes
    Gulshana A Mazumder, Arif Uddin, Supriyo Chakraborty
    Molecular and Biochemical Parasitology.2018; 225: 15.     CrossRef
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  • 179 Download
  • 13 Web of Science
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Original Articles

Two Echinostome Species, Pegosomum bubulcum and Nephrostomum ramosum (Digenea: Echinostomatidae), from an Eastern Cattle Egret, Bubulcus ibis coromandus, in Republic of Korea
Seongjun Choe, Dongmin Lee, Hansol Park, Hyeong-Kyu Jeon, Youngsun Lee, Eunju Kim, Ki-Jeong Na, Keeseon S. Eom
Korean J Parasitol 2016;54(4):485-496.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.485
We describe 2 echinostome species recovered from an Eastern cattle egret, Bubulcus ibis coromandus, from Cheongju-si (city), Chungcheongbuk-do (province), Korea. Total 72 Pegosomum bubulcum specimens were recovered from the bile duct. They were 7,566×2,938 μm in average size and had 27 collar spines with vitelline extension from anterior 1/3 level of the esophagus to mid-level of the posterior testis as characteristic features. Total 9 specimens of Nephrostomum ramosum were recovered in the small intestines of the bird. They were ribbon-shaped, 11,378×2,124 μm in average size, and morphologically variable in some organs, i.e., the number of collar spines (47-50), the shape of ovary and testes, and the extension of vitelline follicles. These morphological variations observed in a single host indicated that these features are not critical for the classification of Nephrostomum species and thus were reconsidered taxonomically as synonym of N. ramosum. This study is the first report documenting and describing both flukes and their associated genera in Korea.

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Biological Parameters of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Fed on Rabbits, Sheep, and Cattle
Miling Ma, Ze Chen, Aihong Liu, Qiaoyun Ren, Junlong Liu, Zhijie Liu, Youquan Li, Hong Yin, Guiquan Guan, Jianxun Luo
Korean J Parasitol 2016;54(3):301-305.
Published online June 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.3.301
In order to determine the effect of various hosts on feeding performance of Rhipicephalus (Boophilus) microplus, we used 3 mammalian species as hosts, cattle (Qinchuan), sheep (T an), and rabbits (Japanese white rabbit) for infest-ing ticks. Five hundreds of R. microplus larvae were exposed to each animal (3 animals/host species). Tick recoveries were 11.0%, 0.47%, and 5.5% from cattle, sheep, and rabbits, respectively. The averages of tick feeding periods were not significantly different on cattle, sheep, and rabbits, 28.8, 25.3, and 26.7 days, respectively. The average weights of individual engorged female from cattle, sheep, and rabbits were 312.5, 219.1, and 130.2 mg, respectively and those of egg mass weights each to 85.0, 96.6, and 17.8 mg. The highest egg hatching rate was in the ticks from cattle (96.0%), fol-lowed by those from rabbits (83.0%) and sheep (19.2%). These data suggest that rabbits could be as an alternative host to cultivate R. microplus for evaluating vaccines and chemical and biological medicines against the tick in the laboratory, although the biological parameters of ticks were less than those from cattle.

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Brief Communication

Identification of Cryptosporidium from Dairy Cattle in Pahang, Malaysia
Nur Hazirah Hisamuddin, Najat Hashim, Sharmeen Nellisa Soffian, Mohd Hishammfariz Mohd Amin, Ridhwan Abdul Wahab, Mardhiah Mohammad, Muhammad Lokman Md Isa, Afzan Mat Yusof
Korean J Parasitol 2016;54(2):197-200.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.197
Cryptosporidium, a protozoan parasite, can cause cryptosporidiosis which is a gastrointestinal disease that can infect humans and livestock. Cattle are the most common livestock that can be infected with this protozoan. This study was carried out to determine the prevalence of Cryptosporidium infection in cattle in Kuantan, Pahang, Malaysia and to find out the association between the occurrence of infection and 3 different ages of cattle (calves less than 1 year, yearling, and adult cattle). The samples were processed by using formol-ether concentration technique and stained by modified Ziehl Neelsen. The results showed that 15.9% (24/151) of cattle were positive for Cryptosporidium oocysts. The occurrence of Cryptosporidium in calves less than 1 year was the highest with the percentage of 20.0% (11/55) followed by yearling and adult cattle, with the percentage occurrence of 15.6 % (7/45) and 11.8% (6/51), respectively. There was no significant association between the occurrence and age of cattle and presence of diarrhea. Good management practices and proper hygiene management must be taken in order to reduce the infection. It is highly important to control the infection since infected cattle may serve as potential reservoirs of the infection to other animals and humans, especially animal handlers.

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  • Prevalence and distribution of Cryptosporidium spp. in cattle in central and western Inner Mongolia, China
    Ziran Mo, Bin Xu, Jingwei Quan, Qinghan Meng, Xu Jiang, Junyan Li, Jia Wang, Yaping Xuan, Xingyu Ruan, Wenbin Yang, Xiaoping Luo, Wei Hu
    BMC Microbiology.2025;[Epub]     CrossRef
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    Mircea Jifcu, Diana Hoffman , Ioan-Cristian Dreghiciu , Alesia Cioacă , Tiana Florea , Anamaria Plesko , Ion Oprescu , Sorin Morariu , Marius Stelian Ilie
    Romanian Journal of Veterinary Sciences.2024; 58(1): 164.     CrossRef
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    Sen Li, Yang Zou, Pei Wang, Ming-Ren Qu, Wen-Bin Zheng, Ping Wang, Xiao-Qing Chen, Xing-Quan Zhu
    Parasitology Research.2021; 120(4): 1281.     CrossRef
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    Asia Pacific Journal of Public Health.2020; 32(1): 8.     CrossRef
  • Molecular detection and epidemiological risk factors associated with Cryptosporidium infection among cattle in Peninsular Malaysia
    D.A. Abdullah, S.D. Ola-Fadunsin, K. Ruviniyia, F.I. Gimba, P. Chandrawathani, Y.A.L. Lim, F.F.A. Jesse, R.S.K. Sharma
    Food and Waterborne Parasitology.2019; 14: e00035.     CrossRef
  • Prevalence and molecular characterization of bovine Cryptosporidium from dairy cows in Northern Thailand
    Tawin Inpankaew, Tawisa Jiyipong, Chainirun Sunanta, Chanya Kengradomkij, Nongnuch Pinyopanuwat, Sathaporn Jittapalapong
    Acta Parasitologica.2017;[Epub]     CrossRef
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Original Article

Prevalence and Associated Risk Factors of Toxocara vitulorum Infections in Buffalo and Cattle Calves in Three Provinces of Central Cambodia
Pierre Dorny, Brecht Devleesschauwer, Val?rie Stoliaroff, Meas Sothy, Rortana Chea, Bunthon Chea, Hor Sourloing, Sum Samuth, Seth Kong, Koemseang Nguong, San Sorn, Davun Holl, Jozef Vercruysse
Korean J Parasitol 2015;53(2):197-200.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.197
The prevalence and associated risk factors of Toxocara vitulorum infection in buffalo and cattle calves was studied in 3 provinces in central Cambodia. Fecal samples were collected from 517 calves between the age of 1-15 weeks and processed for nematode egg counts by a modified McMaster method. A total of 64 calves were found to excrete T. vitulorum eggs in their feces (12.4%; 95% exact CI: 9.7-15.5). The mean fecal egg count was 2,798 EPG (SD=16,351; range=0-224,400). A multivariable generalized linear mixed model showed higher odds of T. vitulorum infection for buffalo versus cattle, for animals aged 4-8 weeks versus younger and older ones, and for animals with strongyle infection. There was no association with fecal consistency. Farmers should be aware of the potential impact of T. vitulorum, and treat their calves at the age of 2-3 weeks with anthelmintics such as benzimidazoles or pyrantel.

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    Parasitology.2024; 151(8): 795.     CrossRef
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    Turkish Journal of Parasitology.2023; 47(2): 88.     CrossRef
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    Research in Veterinary Science.2023; 162: 104952.     CrossRef
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    Mahmoud AbouLaila, Makoto Igarashi, Ahmed ElKhatam, Soad Menshawy
    Veterinary Parasitology: Regional Studies and Reports.2022; 27: 100673.     CrossRef
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    Dicle Üniversitesi Veteriner Fakültesi Dergisi.2022; 15(1): 1.     CrossRef
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  • Prevalence and Therapeutic Efficacy of Anthelmintic against Neoascaris vitulorum in Buffalo Populations from Sylhet District of Bangladesh
    Shishir Kanu, Md. Shahidur Rahman Chowdhury, Md. Abdus Sabur, Md. Mahfujur Rahman, Kazi Mehetazul Islam, Md Bashir Uddn, Md. Rafqul Lslam, Md. Mukter Hossain
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    Veterinary Parasitology: Regional Studies and Reports.2020; 19: 100370.     CrossRef
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    PLOS ONE.2020; 15(5): e0233453.     CrossRef
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    Helminthologia.2020; 57(2): 83.     CrossRef
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    The Korean Journal of Parasitology.2016; 54(5): 645.     CrossRef
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Brief Communication

Ixodid Tick Infestation in Cattle and Wild Animals in Maswa and Iringa, Tanzania
You Shine Kwak, Tae Yun Kim, Sung-Hyun Nam, In-Yong Lee, Hyung-Pyo Kim, Simon Mduma, Julius Keyyu, Robert Fyumagwa, Tai-Soon Yong
Korean J Parasitol 2014;52(5):565-568.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.565

Ticks and tick-borne diseases are important in human and livestock health worldwide. In November 2012, ixodid ticks were collected and identified morphologically from cattle and wild animals in the Maswa district and Iringa urban, Tanzania. Amblyomma gemma, A. lepidum, and A. variegatum were identified from Maswa cattle, and A. variegatum was the predominant species. A. marmoreum, Hyalomma impeltatum, and Rhipicephalus pulchellus were identified from Iringa cattle in addition to the above 3 Amblyomma species, and A. gemma was the most abundant species. Total 4 Amblyomma and 6 Rhipicephalus species were identified from wild animals of the 2 areas. A. lepidum was predominant in Maswa buffaloes, whereas A. gemma was predominant in Iringa buffaloes. Overall, A. variegatum in cattle was predominant in the Maswa district and A. gemma was predominant in Iringa, Tanzania.

Citations

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  • Prevalence of Tick-Borne Pathogens from Ticks Collected from Cattle and Wild Animals in Tanzania in 2012
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Original Article

Phylogenetic Analysis of Ruminant Theileria spp. from China Based on 28S Ribosomal RNA Gene
Huitian Gou, Guiquan Guan, Miling Ma, Aihong Liu, Zhijie Liu, Zongke Xu, Qiaoyun Ren, Youquan Li, Jifei Yang, Ze Chen, Hong Yin, Jianxun Luo
Korean J Parasitol 2013;51(5):511-517.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.511

Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.

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Brief Communications

Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
Fatemeh Selahi, Mehdi Namavari, Mohammad Hossein Hosseini, Maryam Mansourian, Yahya Tahamtan
Korean J Parasitol 2013;51(1):129-132.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.129

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.

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Intestinal Parasite Infections in Pigs and Beef Cattle in Rural Areas of Chungcheongnam-do, Korea
Hassan Ahmed Hassan Ahmed Ismail, Hyung-Kyu Jeon, Yong-Man Yu, Changhee Do, Young-Ha Lee
Korean J Parasitol 2010;48(4):347-349.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.347

The present study was performed to investigate the infection status of intestinal parasites in pigs and beef cattle in rural areas of Chungcheongnam-do, Korea. From November 2009 to April 2010, a total of 241 fecal samples of pigs and beef cattle (136 and 105, respectively) were examined by direct smear and centrifugal sedimentation methods. The overall positive rates of intestinal parasites among pigs and beef cattle were 73.5% and 4.8%, respectively, and the double-infection rate was 10.3% in pigs. Of 136 specimens from pigs, Balantidium coli, Ascaris suum, and Entamoeba spp. infections were found in 88 (64.7%), 24 (17.6%), and 5 cases (3.7%), respectively. Of 105 beef cattle, Entamoeba spp. infections were detected in 5 cases (4.8%). From these results, it is shown that pigs raised on rural farms in Chungcheongnam-do had a high B. coli infection rate and a moderate A. suum infection rate. These results demonstrate that environmentally resistant cysts or eggs could be widespread on the farms examined, and thus an effective hygienic management system is needed to prevent them from serving as the source of infection for human beings.

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Case Report

The First Outbreak of Chorioptes texanus (Acari: Psoroptidae) Infestation in a Cattle Farm in Korea
Guk-Hyun Suh, Tai-Young Hur, Sun Lim, Sang-Min Shin, Jungkee Kwon, Shin-Hyeong Cho, Chai-Yong Lee, Sung-Shik Shin
Korean J Parasitol 2008;46(4):273-278.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.273

Mites in the genus Chorioptes cause a mild form of skin disease in both domestic and wild ruminants. In July 2006, dermatitis characterized by alopecia, marked lichenification, accumulation of crust, and fissuring was recognized in 14 out of 200 Holstein dairy cattle raised in the cattle farm of the National Institute of Animal Science in Cheonan, Republic of Korea. Skin lesions were distributed mainly over the tail base, and sacral and perineal regions. Microscopic examinations of skin scraping samples from severely affected areas revealed numerous mites of all developmental stages. Morphologically, pedicels of the mites were short and unjointed. The tarsal suckers occurred on the pedicels of all the legs in the male worm and on the first, second, and fourth pair of legs in the adult female worm. A single long seta at the tarsus of legs III and the length of legs II being about twice as long as legs IV in adult male mites were observed. Arising anterior to the inner-most spatulate seta was a short seta with an average of 26.4 ± 5.8 ?m in length. Also, the length of setae #4 on the opisthosomal lobes was relatively short. Based on these observations, the mites were identified as Choriptes texanus. Although the chorioptic mange may not influence the mortality rate in the affected farm, reports indicate that a decline in milk production can be observed. This is the first report of chorioptic infestation in a cattle farm from Korea.

Citations

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    Asuka Yamashiro, Kozue Yamashiro, Moegi Jyo, Tadashi Yamashiro
    Mammal Study.2024;[Epub]     CrossRef
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    V. Melnychuk, S. Kovalenko
    Bulletin "Veterinary biotechnology".2023; (43): 124.     CrossRef
  • Use of a mechanical brush by dairy cows with chorioptic mange
    Ana C. Moncada, Heather W. Neave, Marina A.G. von Keyserlingk, Daniel M. Weary
    Applied Animal Behaviour Science.2020; 223: 104925.     CrossRef
  • Transcriptome-based analysis of putative allergens of Chorioptes texanus
    Ran He, Xiao-Bin Gu, Yue Xie, Xue-Rong Peng, Christiana Angel, Guang-You Yang
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Integrated Bayesian species delimitation and morphological diagnostics of chorioptic mange mites (Acariformes: Psoroptidae: Chorioptes)
    Andre V. Bochkov, Pavel B. Klimov, Gete Hestvik, Alexander P. Saveljev
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    Xiang Nong, Shu-Hua Li, Jia-Hai Wang, Yue Xie, Feng-Zheng Chen, Tian-Fei Liu, Ran He, Xiao-Bin Gu, Xue-Rong Peng, Guang-You Yang
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Brief Communication

Prevalence of ixodid ticks on cattle in Mazandaran province, Iran
Gholam Reza Razmi, Meisam Glinsharifodini, Shaboddin Sarvi
Korean J Parasitol 2007;45(4):307-310.
Published online December 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.4.307

A survey was carried out to investigate the prevalence of hard tick species (Acari: Ixodidae) on cattle in Mazandaran province, Iran. A total of 953 ticks were collected from 86 infested cattle during activating seasons of ticks during 2004-2005. Nine species were identified: Boophilus annulatus (51.3%), Rhipicephalus bursa (16.8%), Haemaphysalis punctata (6.3%), Ixodes ricinus (6.8%), Hyalomma marginatum (12.5%), Hyalomma anatolicum excavatum (5.2%), Hyalomma asiaticum (0.6%), Hyalomma detritum (0.2 %), and Dermacentor spp. (0.1%). The results show that Boophilus annulatus, Rhipicephalus bursa, and Hyalomma species are dominant tick species in the surveyed area.

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Original Articles

Treatment of natural tropical theileriosis with the extract of the plant Peganum harmala
Mohammad Mirzaei
Korean J Parasitol 2007;45(4):267-271.
Published online December 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.4.267

Theileria annulata, a protozoan parasite of cattle and domestic buffaloes, is transmitted by ticks of the genus Hyalomma, and causes a disease named Mediterranean or tropical theileriosis. In this research 50 cattle naturally infected with Theileria annulata were treated with the extract of the plant Peganum harmala. The treatment was continued for 5 days, the dose of the extract being 5 mg/kg per day. After the treatment, 39 cattle responded to the treatment and recovered, but 11 did not respond to the treatment and died. The recovery rate of animals treated with the extract of the plant Peganum harmala was 78%.

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    Barbara Huber, Marta Luciani, Ahmed M. Abualhassan, Daniel Giddings Vassão, Ricardo Fernandes, Thibaut Devièse
    Communications Biology.2025;[Epub]     CrossRef
  • Therapeutic evaluation of Buparvaquone and Peganum harmala against theileriosis in cattle
    S. A. Lokhande, M. F. M. F. Siddiqui, M. P. Sakhare, G. M. Chigure, N. D. Jadhav, S. Sajid Ali, S. D. Chepte, S. R. Shaikh, P. C. Ahire
    Tropical Animal Health and Production.2025;[Epub]     CrossRef
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  • Prevalence and risk factors assessment of theileriosis in livestock of Malakand Division, Pakistan
    Mohsinullah Mohsin, Khalid Hameed, Mustafa Kamal, Asad Ali, Naseem Rafiq, Tahir Usman, Wali Khan, Ansar Ahmed Abbasi, Rahmat Ullah Khan, Gulalai Jamal Yousafzai
    Journal of the Saudi Society of Agricultural Sciences.2022; 21(4): 242.     CrossRef
  • Antibacterial, Antifungal, Antiviral, and Antiparasitic Activities of Peganum harmala and Its Ingredients: A Review
    Zihao Zhu, Shujuan Zhao, Changhong Wang
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    Liang Guo, Wei Chen, Rihui Cao, Wenxi Fan, Qin Ma, Jie Zhang, Bin Dai
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  • In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica
    Mohammad Moazeni, Zahra Sadat Saadaty Ardakani, Mohammad Jamal Saharkhiz, Jafar Jalaei, Ali Asghar Khademolhoseini, Shahab Shams Esfand Abad, Amir Mootabi Alavi
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    M B CHHABRA, K M L PATHAK, K MURALEEDHARAN
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    A.P.R. Saber, M. Khorrami, M. Nouri
    International Journal of Dairy Science.2008; 3(4): 205.     CrossRef
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Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea
Jae-Hwan Park, Sang-Mee Guk, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Jong-Yil Chai
Korean J Parasitol 2006;44(1):27-33.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.27

Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified Ziehl-Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases constituted 7 (41.2%) of 17 examined, and goat cases 3 (42.9%) of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.

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Brief Communication

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam
Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.3.175

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

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Original Article

Prevalence of Theileria sergenti infection in Korean native cattle by polymerase chain reaction
Kun-Ho Song, Byung-Chan Sang
Korean J Parasitol 2003;41(3):141-145.
Published online September 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.3.141

This study was performed to investigate the prevalence of theileriosis and to compare the prevalence of this disease in Korean native cattle reared under different environmental conditions, namely, in a grazing area and a non-grazing area by polymerase chain reaction. Three hundred and one Korean native cattle (276 cows and 25 bulls) that had not received prior treatment or been vaccinated to prevent theileriosis were examined by PCR for Theileria sergenti infection from 2001 to 2002. In our study, the parasitemia range in T. sergenti-positive cattle by microscopy were from 0.1 to 3% (mean 0.8%). In terms of mean prevalence, 204 of the 301 Korean native cattle (67.8%) were positive reaction by PCR. Our results also revealed that the infection rate among cows (70.3%) was significantly higher than that among bulls (40.0%) (p < 0.01). T. sergenti infection among the over 3 year-old-group (75%) had a significant higher prevalence than that among the less than 3 year-old-group (61.8%) (p < 0.05). Our data also showed that grazing areas (76.1%) had the significant higher prevalence than non-grazing areas (51%) (p < 0.001). In conclusion, this study demonstrates that the prevalence of T. sergenti infection is high and that its prevalence in grazing cattle is higher than that in non-grazing cattle. Therefore, life-long treatment and the development of an optimal vaccine are needed to reduce the numbers of bovine theileriosis in both grazing and non-grazing areas.

Citations

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    F. Tatsukawa
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  • SEROLOGICAL INVESTIGATION OF THEILERIA SERGENTI USING LATEX AGGLUTINATION TEST IN SOUTH KOREA
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Mini Review

Review of Neospora caninum and neosporosis in animals
J.P. Dubey
Korean J Parasitol 2003;41(1):1-16.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.1

Neospora caninum is a coccidian parasite of animals. It is a major pathogen for cattle and dogs and it occasionally causes clinical infections in horses, goats, sheep, and deer. Domestic dogs are the only known definitive hosts for N. caninum. It is one of the most efficiently transmitted parasite of cattle and up to 90% of cattle in some herds are infected. Transplacental transmission is considered the major route of transmission of N. caninum in cattle. Neospora caninum is a major cause of abortion in cattle in many countries. To elicit protective immunity against abortion in cows that already harbor a latent infection is a major problem. This paper reviews information on biology, diagnosis, epidemiology and control of neosporosis in animals.

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Original Articles
Experimental induction of the two-host life cycle of Sarcocystis cruzi between dogs and Korean native calves
Sung-Hwan Wee, Sung-Shik Shin
Korean J Parasitol 2001;39(3):227-232.
Published online September 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.3.227

Eight dogs were experimentally infected with Sarcocystis by oral inoculation of cardiac muscle from naturally infected cattle. The infected dogs commenced discharging of sporocysts in the feces after 10 to 12 days of inoculation, and continued until 20 and 35 days after inoculation. Three dogs were reinfected with cardiac muscle from the naturally infected cattle. Sporocysts reappeared in the feces on 12 to 13 days after reinfection. Sarcosystis sporocysts collected from the experimentally infected dogs were fed to each of the two 30-day-old Korean native calves. The infected calves remained clinically normal, except for the high fever (≥ 40℃) and decreased hematocrit values on day 30 to 40 post inoculation. Muscular cysts of Sarcocystis were found from infected calves on day 40 post inoculation. Proliferative forms of Sarcocystis were also observed in the muscle of infected calves. These results suggest that the Sarcocystis cruzi found in Korean native cattle has a 2-host life cycle with dogs as the definitive host and Korean native calves as the intermediate host.

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Detection of IgG antibody against Neospora caninum in cattle in Korea
Ji-Seon Bae, Dae-Yong Kim, Woo-Suk Hwang, Jae-Hoon Kim, Nam-Seok Lee, Ho-Woo Nam
Korean J Parasitol 2000;38(4):245-249.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.245

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

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