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Original Articles

Molecular survey of Toxoplasma gondii B1 gene in pigs from various localities in Korea
Dongmi Kwak, Min-Goo Seo
Parasites Hosts Dis 2024;62(3):294-301.
Published online August 26, 2024
DOI: https://doi.org/10.3347/PHD.24031
Toxoplasma gondii, a common protozoan parasite, poses significant public health risks due to its potential to cause toxoplasmosis in humans and can be contracted from pigs, which are considered its critical intermediate host. The aim of this study is to evaluate the prevalence of T. gondii in slaughtered pigs for human consumption, emphasizing the zoonotic implications and the need for improved biosecurity and monitoring practices in pig farming. A total of 1,526 pig samples (1,051 whole blood samples and 384 lung tissue samples from the local slaughterhouse and 91 aborted fetus samples from local farms) were collected throughout the whole country of Korea in 2020. Among them, 6 (0.4%) were found to be infected with T. gondii by nested PCR. When compared by sample type, the prevalence of T. gondii was significantly higher in the aborted fetus samples (2.2%, 2/91) than in the blood (0.3%, 3/1,051) and lung tissue samples (0.3%, 1/384). The B1 gene sequence of T. gondii was similar (97.9–99.8%) to that of the other T. gondii isolates. This study represents the first molecular genotyping survey of T. gondii in the lung tissue of fattening pigs and aborted fetuses in Korea. Our findings indicated the importance of adopting preventive measures including the implementation of rigorous farm hygiene protocols and the promotion of public awareness about the risks of consuming undercooked pork. By addressing the gaps in current control strategies and encouraging the One Health approach, this study contributes to the development of more effective strategies to mitigate the transmission of T. gondii from pigs to humans, ultimately safeguarding public health.
  • 2,621 View
  • 69 Download
  • 1 Web of Science
Geographical Distribution and Epidemiologic Factors of Chigger Mites on Apodemus agrarius during Autumn in Korea
In Yong Lee, Jae-Won Lim, Jang Hoon Seo, Heung Chul Kim, Ku Jae Lee, Tai-Soon Yong, Won-Ja Lee, Jae-Ran Yu, Seobo Sim
Korean J Parasitol 2021;59(5):473-479.
Published online October 22, 2021
DOI: https://doi.org/10.3347/kjp.2021.59.5.473
A nationwide survey of chigger mites causing scrub typhus and an investigation of epidemiologic factors for chigger mites was conducted at 16 localities in 8 provinces in Korea during autumn 2009, 2012, and 2013. A total of 233 Apodemus agrarius were captured, and all were infested with chigger mites. The chigger index was highest in Chungcheongbuk-do in 2009 (358.3) and 2012 (290.1) and Chungcheongnam-do in 2013 (294.4). The predominant chigger mite species was Leptotrombidium pallidum in the northern and central parts and L. scutellare in the southern and western parts, Korea. L. pallidum was not found in Jellanam-do and Gyeongsangnam-do and the distribution of L. scutellare had been expanded in the northern parts of Korea. The chigger index of L. pallidum was positively correlated with temperature and negatively correlated with humidity. The incidence of scrub typhus is dependent on L. scutellare index. These findings could be helpful to monitor the distribution of chigger mites and to develop a preventive measures for scrub typhus in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Scrub typhus update: A re‑emerging global threat beyond the Tsutsugamushi Triangle and the physiological ramifications of scrub typhus infection (Review)
    Ankur Vashishtha, Vivek Kumar, Gautam Panwar, Gaurav Kausik, Samaniya Baig, Prigya Sharma, Rajesh Yadav
    World Academy of Sciences Journal.2025;[Epub]     CrossRef
  • The Association Between Environmental Factors and Scrub Typhus: A Review
    Shu Yang, Shu Yang, Yuxiang Xie, Wenjing Duan, Yiting Cui, Ai Peng, Yisheng Zhou, Yibing Fan, Hui Li, Peng Huang
    Tropical Medicine and Infectious Disease.2025; 10(6): 151.     CrossRef
  • Temperature effect on scrub typhus incidence in South Korea: the projection for climate change
    Donghee Seo, Yoon-Jung Choi, Yun-Chul Hong
    Postgraduate Medical Journal.2025;[Epub]     CrossRef
  • Associations of meteorological factors and dynamics of scrub typhus incidence in South Korea: A nationwide time-series study
    Taehee Chang, Kyung-Duk Min, Sung-il Cho, Yoonhee Kim
    Environmental Research.2024; 245: 117994.     CrossRef
  • The impact of meteorological parameters on the scrub typhus incidence in Baoshan City, western Yunnan, China
    Yun-Yan Luo, Alan Frederick Geater, Jia-Xiang Yin
    Frontiers in Public Health.2024;[Epub]     CrossRef
  • Prevalence of chigger mites and Orientia tsutsugamushi strains in northern regions of Gangwon-do, Korea
    Soojin Kim, In Yong Lee, Sezim Monoldorova, Jiro Kim, Jang Hoon Seo, Tai-Soon Yong, Bo Young Jeon
    Parasites, Hosts and Diseases.2023; 61(3): 263.     CrossRef
  • Molecular Identification of Bacillus Isolated from Korean Water Deer (Hydropotes inermis argyropus) and Striped Field Mouse (Apodemus agrarius) Feces by Using an SNP-Based 16S Ribosomal Marker
    Md-Mafizur Rahman, Sang-Jin Lim, Yung-Chul Park
    Animals.2022; 12(8): 979.     CrossRef
  • 5,408 View
  • 132 Download
  • 7 Web of Science
  • Crossref

Mini Review

Acanthamoeba in Southeast Asia ? Overview and Challenges
Chooseel Bunsuwansakul, Tooba Mahboob, Kruawan Hounkong, Sawanya Laohaprapanon, Sukhuma Chitapornpan, Siriuma Jawjit, Atipat Yasiri, Sahapat Barusrux, Kingkan Bunluepuech, Nongyao Sawangjaroen, Cristina C. Salibay, Chalermpon Kaewjai, Maria de Lourdes Pereira, Veeranoot Nissapatorn
Korean J Parasitol 2019;57(4):341-357.
Published online August 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.4.341
Acanthamoeba, one of free-living amoebae (FLA), remains a high risk of direct contact with this protozoan parasite which is ubiquitous in nature and man-made environment. This pathogenic FLA can cause sight-threatening amoebic keratitis (AK) and fatal granulomatous amoebic encephalitis (GAE) though these cases may not commonly be reported in our clinical settings. Acanthamoeba has been detected from different environmental sources namely; soil, water, hot-spring, swimming pool, air-conditioner, or contact lens storage cases. The identification of Acanthamoeba is based on morphological appearance and molecular techniques using PCR and DNA sequencing for clinico-epidemiological purposes. Recent treatments have long been ineffective against Acanthamoeba cyst, novel anti-Acanthamoeba agents have therefore been extensively investigated. There are efforts to utilize synthetic chemicals, lead compounds from medicinal plant extracts, and animal products to combat Acanthamoeba infection. Applied nanotechnology, an advanced technology, has shown to enhance the anti-Acanthamoeba activity in the encapsulated nanoparticles leading to new therapeutic options. This review attempts to provide an overview of the available data and studies on the occurrence of pathogenic Acanthamoeba among the Association of Southeast Asian Nations (ASEAN) members with the aim of identifying some potential contributing factors such as distribution, demographic profile of the patients, possible source of the parasite, mode of transmission and treatment. Further, this review attempts to provide future direction for prevention and control of the Acanthamoeba infection.

Citations

Citations to this article as recorded by  Crossref logo
  • Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.
    Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Alok K. Paul, Sonia M. Rodrigues Oliveira, Maria L. Pereira, Tajudeen O. Jimoh, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Ajoy K. Verma, Veeranoot Nissapatorn
    Veterinary World.2025; : 110.     CrossRef
  • Acanthamoeba castellanii–Mediated Reduction of Interleukin‐1β Secretion and Its Association With Macrophage Autophagy
    Rachasak Boonhok, Wilaiwan Senghoi, Suthinee Sangkanu, Chooi Ling Lim, Matsayapan Pudla, Maria de Lourdes Pereira, Polrat Wilairatana, Tooba Mahboob, Md. Atiar Rahman, Pongsak Utaisincharoen, Poonsit Hiransai, Veeranoot Nissapatorn, Simone Carradori
    Scientifica.2025;[Epub]     CrossRef
  • Comparative evaluation of nano ocular delivery systems loaded pH and thermosensitive in situ gels for Acanthamoeba keratitis treatment
    Basant A. Abou-Taleb, Ibrahim A. Abdelwahab
    Scientific Reports.2025;[Epub]     CrossRef
  • Amoebicidal, anti-adhesive, and low-cytotoxic effects of Mangifera indica L. leaf extract against ocular Acanthamoeba spp.: First evidence supporting plant-based therapeutic potential
    Diana Mendonça, Hazel A. Tabo, Siriphorn Chimplee, Sónia M. R. Oliveira, Pattamaporn Kwankaew, Ana Paula Girol, Julieta Z. Dungca, Mazdida Sulaiman, Subha Bhassu, Muhammad Nawaz, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Sunil Kayesth, Veerano
    Veterinary World.2025; : 3322.     CrossRef
  • Azole-based compounds as potential anti-Acanthamoeba agents
    Balsam Qubais Saeed, Rania Hamdy, Noor Akbar, Sreedevi Edathadan Sajeevan, Naveed Ahmed Khan, Sameh S. M. Soliman
    RSC Medicinal Chemistry.2024; 15(5): 1578.     CrossRef
  • In silico-based vaccine design against Naegleria fowleri causing primary amebic meningoencephalitis
    Vityala Yethindra, Sai Kiran Ramanujam, Dharani Mummadi, Krishna Priya Kanteti, Srilaxmi Vityala, Ujwala Damineni
    Indian Journal of Pharmacology.2024; 56(2): 148.     CrossRef
  • Unfurling a case of encephalitis with Acanthamoeba after a near-drowning event
    Debarup Das, Kuntal Biswas, Kokila Banerjee, Bhaswar Bhattacharya, Arijit Roy, Sumeeta Khurana, Atanu Biswas
    Rare.2024; 2: 100035.     CrossRef
  • A Comprehensive Review on Acanthamoeba Keratitis: An Overview of Epidemiology, Risk Factors, and Therapeutic Strategies
    Diksha Garg, Sachin Daigavane
    Cureus.2024;[Epub]     CrossRef
  • First report on the long-term viability of Acanthamoeba species in unpreserved environmental freshwater samples stored at room temperature
    Frederick R. Masangkay, Fausto C. Rona IV, Angel Jasmine G. Poricallan, Raphael Louis M. Padua, Marielle Sabina S. Reyes, Justine Kate M. Ricacho, Monica Jane E. Samar, Maria Carmela I. Santos, Jitbanjong Tangpong, Manas Kotepui, Mark F. F. E. Padua, Giov
    Journal of Water and Health.2024; 22(11): 2194.     CrossRef
  • A Narrative Review of Acanthamoeba Isolates in Malaysia: Challenges in Infection Management and Natural Therapeutic Advancements
    Mohammad Wisman Abdul Hamid, Roslaini Bin Abd Majid, Victor Fiezal Knight Victor Ernest, Nik Noorul Shakira Mohamed Shakrin, Firdaus Mohamad Hamzah, Mainul Haque
    Cureus.2024;[Epub]     CrossRef
  • Occurrence, molecular diversity and pathogenicity of Acanthamoeba spp. isolated from aquatic environments of Northeastern Brazil
    Yrna Lorena Matos de Oliveira, Erica Tirzah Santos Lima, Marilise Brittes Rott, Roberta Pereira Miranda Fernandes, Sona Jain, Marcus Vinicius de Aragão Batista, Silvio Santana Dolabella
    International Journal of Environmental Health Research.2023; 33(12): 1686.     CrossRef
  • Molecular characterisation and potential pathogenicity analysis of Acanthamoeba isolated from recreational lakes in Peninsular Malaysia
    Rohaya Abdul Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Hasseri Halim, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
    Journal of Water and Health.2023; 21(9): 1342.     CrossRef
  • Applications of medicinal chemistry for drug discovery against Acanthamoeba infections
    Usman Ahmed, Ayaz Anwar, Seng‐Kai Ong, Areeba Anwar, Naveed Ahmed Khan
    Medicinal Research Reviews.2022; 42(1): 462.     CrossRef
  • Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution
    Suthinee Sangkanu, Watcharapong Mitsuwan, Tooba Mahboob, Wilawan Mahabusarakam, Sopa Chewchanwuttiwong, Panisara Siphakdi, Tajudeen O. Jimoh, Polrat Wilairatana, Karma G. Dolma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Roghayeh No
    Acta Tropica.2022; 226: 106266.     CrossRef
  • Acanthamoeba species isolated from marine water in Malaysia exhibit distinct genotypes and variable physiological properties
    Rosnani Hanim Mohd Hussain, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
    Journal of Water and Health.2022; 20(1): 54.     CrossRef
  • Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
    Li-Li Chan, Hiu-Ching Toh, Kantiya Jaikwang, Ee-Mun Loo, Jia-Haw Wong, Yun-Khoon Liew, Kian-Huat Ong, Soo-Shen Ooi
    Acta Tropica.2022; 229: 106372.     CrossRef
  • Tannic Acid-Modified Silver Nanoparticles in Conjunction with Contact Lens Solutions Are Useful for Progress against the Adhesion of Acanthamoeba spp. to Contact Lenses
    Marcin Padzik, Lidia Chomicz, Julita Bluszcz, Karolina Maleszewska, Jaroslaw Grobelny, David Bruce Conn, Edyta B. Hendiger
    Microorganisms.2022; 10(6): 1076.     CrossRef
  • Isolation and morphological and molecular characterization of waterborne free-living amoebae: Evidence of potentially pathogenic Acanthamoeba and Vahlkampfiidae in Assiut, Upper Egypt
    Martina M. Nageeb, Hanan E. M. Eldeek, Rasha A. H. Attia, Atef A. Sakla, Samia S. Alkhalil, Haiam Mohamed Mahmoud Farrag, Ebrahim Shokoohi
    PLOS ONE.2022; 17(7): e0267591.     CrossRef
  • Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis
    Beni Jequicene Mussengue Chaúque, Denise Leal dos Santos, Davood Anvari, Marilise Brittes Rott
    Parasitology Research.2022; 121(11): 3033.     CrossRef
  • Identification of Acanthamoeba spp. from water and soil of public parks in the north of Iran
    M. R. Mahmoudi, M. Maleki, N. Zebardast, B. Rahmati, K. Ashrafi, M. Sharifdini, Panagiotis Karanis
    Journal of Water and Health.2022; 20(10): 1604.     CrossRef
  • Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites
    Hương-Giang Lê, Ji-Su Choi, Buyng-Su Hwang, Yong-Tae Jeong, Jung-Mi Kang, Tuấn-Cường Võ, Pyo-Yun Cho, Young-Kyung Lee, Won-Gi Yoo, Yeonchul Hong, Young-Taek Oh, Byoung-Kuk Na
    Plants.2022; 11(24): 3459.     CrossRef
  • Oral Miltefosine as Salvage Therapy for Refractory Acanthamoeba Keratitis
    Praneetha Thulasi, Hajirah N. Saeed, Christopher J. Rapuano, Joshua H. Hou, Alpheus B. Appenheimer, James Chodosh, Joann J. Kang, Amber M. Morrill, Neil Vyas, Michael E. Zegans, Richard Zuckerman, Elmer Y. Tu
    American Journal of Ophthalmology.2021; 223: 75.     CrossRef
  • Photolysis of sodium chloride and sodium hypochlorite by ultraviolet light inactivates the trophozoites and cysts of Acanthamoeba castellanii in the water matrix
    Beni J. M. Chaúque, Marilise B. Rott
    Journal of Water and Health.2021; 19(1): 190.     CrossRef
  • Derivatisation of metronidazole enhances cytotoxic effect against Acanthamoeba genotype T4 isolates and leads to cytomorphological changes in trophozoites
    Aneta Dušeková, Mária Garajová, Miloš Lukáč, Martin Mrva
    Acta Tropica.2021; 216: 105830.     CrossRef
  • Evaluation of the occurrence of pathogenic free‐living amoeba and bacteria in 20 public indoor swimming pool facilities
    María Reyes‐Batlle, Marta F. Gabriel, Rubén Rodríguez‐Expósito, Fátima Felgueiras, Ines Sifaoui, Zenaida Mourão, Eduardo de Oliveira Fernandes, José E. Piñero, Jacob Lorenzo‐Morales
    MicrobiologyOpen.2021;[Epub]     CrossRef
  • Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms
    Suthinee Sangkanu, Watcharapong Mitsuwan, Wilawan Mahabusarakam, Tajudeen O. Jimoh, Polrat Wilairatana, Ana Paula Girol, Ajoy K. Verma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Abolghasem Siyadatpanah, Roghayeh Norouzi, Polydor Ng
    Scientific Reports.2021;[Epub]     CrossRef
  • Silver Nanoparticles Conjugated with Contact Lens Solutions May Reduce the Risk of Acanthamoeba Keratitis
    Edyta B. Hendiger, Marcin Padzik, Inés Sifaoui, María Reyes-Batlle, Atteneri López-Arencibia, Diana Zyskowska, Marta Grodzik, Anna Pietruczuk-Padzik, Jacek Hendiger, Gabriela Olędzka, Lidia Chomicz, José E. Piñero, Jacob Lorenzo-Morales
    Pathogens.2021; 10(5): 583.     CrossRef
  • Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts
    Watcharapong Mitsuwan, Chea Sin, Samell Keo, Suthinee Sangkanu, Maria de Lourdes Pereira, Tajudeen O. Jimoh, Cristina C. Salibay, Muhammad Nawaz, Roghayeh Norouzi, Abolghasem Siyadatpanah, Christophe Wiart, Polrat Wilairatana, Polydor Ngoy Mutombo, Veeran
    Heliyon.2021; 7(5): e06976.     CrossRef
  • Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
    Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel
    Parasitology.2021; 148(9): 1074.     CrossRef
  • Contact lenses contamination by Acanthamoeba spp. in Upper Egypt
    Faten A. M. Hassan, M. E. M. Tolba, Gamal H. Abed, H. M. Omar, Sara S. Abdel-Hakeem, Mohammad Mehdi Feizabadi
    PLOS ONE.2021; 16(11): e0259847.     CrossRef
  • Various brain-eating amoebae: the protozoa, the pathogenesis, and the disease
    Hongze Zhang, Xunjia Cheng
    Frontiers of Medicine.2021; 15(6): 842.     CrossRef
  • Acanthamoeba encephalitis in immunocompetent hosts: A report of two cases
    Sohini Das, Karthik Gunasekaran, SitaraS. R Ajjampur, Dilip Abraham, Tina George, MAsisha Janeela, Ramya Iyadurai
    Journal of Family Medicine and Primary Care.2020; 9(2): 1240.     CrossRef
  • Acanthamoeba spp. monoclonal antibody against a CPA2 transporter: a promising molecular tool for acanthamoebiasis diagnosis and encystment study
    Michele Martha Weber-Lima, Bianca Prado-Costa, Alessandra Becker-Finco, Adriana Oliveira Costa, Philippe Billilad, Cinthia Furst, Juliana Ferreira de Moura, Larissa Magalhães Alvarenga
    Parasitology.2020; 147(14): 1678.     CrossRef
  • Molecular detection of Acanthamoeba spp. in Seven Crater Lakes of Laguna, Philippines
    Lea D. Ballares, Frederick R. Masangkay, Joseph Dionisio, Oliver Villaflores, Maria Ruth Pineda-Cortel, Giovanni D. Milanez
    Journal of Water and Health.2020; 18(5): 776.     CrossRef
  • Effectiveness and Safety of Topical Chlorhexidine and Vitamin E TPGS in the Treatment of Acanthamoeba Keratitis: A Survey on 29 Cases
    Ciro Caruso, Daniela Eletto, Michele Rinaldi, Luigi Pacente, Salvatore Troisi, Francesco Semeraro, Roberto dell’Omo, Ciro Costagliola
    Journal of Clinical Medicine.2020; 9(11): 3775.     CrossRef
  • 13,307 View
  • 313 Download
  • 34 Web of Science
  • Crossref

Original Article

Tick-Borne Pathogens in Ixodid Ticks from Poyang Lake Region, Southeastern China
Wei Qing Zheng, Xue Nan Xuan, Ren Long Fu, Hui Ying Tao, Yang Qing Liu, Xiao Qing Liu, Dong Mei Li, Hong Mei Ma, Hai Ying Chen
Korean J Parasitol 2018;56(6):589-596.
Published online December 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.6.589
Ticks are the vectors of various pathogens, threatening human health and animal production across the globe. Here, for the first time we detected Ricketssia spp., Borrelia spp. and protozoan in ticks from Poyang Lake region in Ji- angxi Province of eastern China. In 3 habitat categories and on 12 host species, 311 ticks from 11 species were collected. Haemaphysalis longicornis was the predominant species, accounting for 55.63%, followed by Rhipicephalus microplus, Haemaphysalis flava and Ixodes granulatus. Of the collected ticks, 7.07% were positive for tick-borne pathogens, and H. longicornis and H. flava were found to be co-infected with Ricketssia spp. and protozoan. H. flava was the most detected positive for tick-borne pathogens, whereas H. longicornis had the lowest infection rate, and the difference in infection rates between tick species was significant (χ2=61.24, P<0.001). Furthermore, adult ticks demonstrated remarkably greater infection rate than immature ticks (χ2=10.12, P=0.018), meanwhile ticks on Erinaceidae showed significantly higher positivity than ticks collected on other host species (χ2=108.44, P<0.001). Genetic fragment sequencing and analyses showed at least 4 pathogen species presence in ticks, namely Borrelia yangtzensis, Rickettsia slovaca or Rickettsia raoultii related genospecies, Babesia vogeli and Hepatozoon canis or Hepatozoon felis related genospecies. The finding indicates that the abundant ticks can carry diverse pathogens in Poyang Lake region, and pathogen infection is highly related to species, vertebrate hosts and life stages of ticks.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular investigation reveals three hemotropic mycoplasmas in cats and three tick species in China
    Hongfei Shi, Guoguang Li, Dandan Li, Hongyue Zhai, Shidong Ji, Yun Hu, Long Wang, Lunguang Yao
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Review of ticks of the order Mesostigmata parasitic on reptiles
    V. R. Saitov, A. H. Gubeidullina, A. I. Golubev, M. M. Salnikova, L. V. Malutina, N. V. Shakurova, I. R. Nigmetzyanov, A. S. Elovitskaya, G. S. Kashevarov, K. A. Yusupova, A. N. Siben, E. A. Efremova, O. M. Bonina, E. A. Udaltsov
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    Stefania Porcelli, Delphine Le Roux, Aurélie Heckmann, Clémence Galon, Lourdes Mateos-Hernandez, Ladislav Šimo, Alejandro Cabezas-Cruz, Anne-Claire Lagrée, Grégory Karadjian, Pierre Lucien Deshuillers, Sara Moutailler
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  • Pathogenetic identification in ticks and yaks from Zoige County, China
    Yang Xiang, Liang He, Liangquan Zhu, Chendong Xiao, Yao Pan, Tianxiang Chen, Wei Zheng, Dongbo Yuan, Lili Hao
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Tick (Acari: Ixodoidea) fauna and zoogeographic division of Jiangxi Province, China
    Jun-Hua Tian, Kun Li, Shao-Zai Zhang, Zhong-Ji Xu, Hai-Xia Wu, Hong-Bin Xu, Chao-Liang Lei
    Ticks and Tick-borne Diseases.2023; 14(2): 102099.     CrossRef
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    Xiao-Qian Cao, Xiao-Lan Gu, Li Zhang, Jiao Xu, Hui-ju Han, Xue-jie Yu
    Ticks and Tick-borne Diseases.2023; 14(3): 102137.     CrossRef
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    Wojciech Zygner, Olga Gójska-Zygner, Justyna Bartosik, Paweł Górski, Justyna Karabowicz, Grzegorz Kotomski, Luke J. Norbury
    Animals.2023; 13(16): 2612.     CrossRef
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    Weijuan Jia, Si Chen, Shanshan Chi, Yunjiang He, Linzhu Ren, Xueli Wang
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  • Protein profiling of hemolymph in Haemaphysalis flava ticks
    Lei Liu, Fen Yan, Lu Zhang, Zhi-feng Wu, De-yong Duan, Tian-yin Cheng
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Detection and phylogenetic analysis of tick-borne bacterial and protozoan pathogens in a forest province of eastern China
    Haijun Hu, Zhanbin Liu, Renlong Fu, Yangqing Liu, Hongmei Ma, Weiqing Zheng
    Acta Tropica.2022; 235: 106634.     CrossRef
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    Baoshan Lin, Yin Ta, Lili Hao
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Wild Hedgehogs and Their Parasitic Ticks Coinfected with Multiple Tick-Borne Pathogens in Jiangsu Province, Eastern China
    Yong Qi, Lele Ai, Changqiang Zhu, Fuqiang Ye, Ruichen Lv, Junhu Wang, Yingqing Mao, Nianhong Lu, Weilong Tan, Biao He
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Detection of Novel Spotted Fever Group Rickettsiae (Rickettsiales: Rickettsiaceae) in Ticks (Acari: Ixodidae) in Southwestern China
    Qian Wang, Wen-Bin Guo, Yu-Sheng Pan, Bao-Gui Jiang, Chun-Hong Du, Teng-Cheng Que, Lin Zhan, Jia-Hong Wu, Ming-Hui Yu, Xiao-Ming Cui, Lin Zhao, Da-Li Xu, Luo-Yuan Xia, Run-Ze Ye, Jie Li, Lian-Feng Li, Wei Wei, Yu-Hao Zhou, Jia-Fu Jiang, Na Jia, Wu-Chun Ca
    Journal of Medical Entomology.2021; 58(3): 1363.     CrossRef
  • Detection of Multiple Intracellular Bacterial Pathogens in Haemaphysalis flava Ticks Collected from Hedgehogs in Central China
    Li-Zhu Fang, Si-Cong Lei, Zhi-Jian Yan, Xiao Xiao, Jian-Wei Liu, Xiao-Qing Gong, Hao Yu, Xue-Jie Yu
    Pathogens.2021; 10(2): 115.     CrossRef
  • Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti
    Weiqing Zheng, Rika Umemiya-Shirafuji, Qian Zhang, Kiyoshi Okado, Paul Franck Adjou Moumouni, Hiroshi Suzuki, Haiying Chen, Mingming Liu, Xuenan Xuan
    Frontiers in Physiology.2020;[Epub]     CrossRef
  • Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China
    Wen-Ping Guo, Guang-Cheng Xie, Dan Li, Meng Su, Rui Jian, Luan-Ying Du
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Identification of Haemaphysalis longicornis Genes Differentially Expressed in Response to Babesia microti Infection
    Weiqing Zheng, Rika Umemiya-Shirafuji, Shengen Chen, Kiyoshi Okado, Paul Franck Adjou Moumouni, Hiroshi Suzuki, Shu Yang, Mingming Liu, Xuenan Xuan
    Pathogens.2020; 9(5): 378.     CrossRef
  • Identification and molecular analysis of Ixodid ticks (Acari: Ixodidae) infesting wild boars (Sus scrofa) and tick-borne pathogens at the Meihua mountain of southwestern Fujian, China
    Xin Wang, Xiaoshuang Sun, Yankuo Sun, Kexin Chen, Kaiyao Zhang, Weihua Xu, Kewei Fan, Weiming Lin, Tengteng Chen, Xipan Lin, Kaixiong Lin, Hung-chuan Chiu, Cuiqin Huang
    Veterinary Parasitology: Regional Studies and Reports.2020; 22: 100492.     CrossRef
  • 6,916 View
  • 131 Download
  • 19 Web of Science
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Brief Communication

A Retrospective Analysis on the Cystic Echinococcosis Cases Occured in Northeastern Punjab Province, Pakistan
Aisha Khan, Sarmad Zahoor, Haroon Ahmed, Uzma Malik, Rabia Amin Butt, Muhammad Sultanul Muzam, Seyma Gunyakti Kilinc, Noorulain Noor, Saroosh Zahoor, Muhammad Sohail Afzal, Hamza Mansur, Shumaila Irum, Sami Simsek
Korean J Parasitol 2018;56(4):385-390.
Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.385
A retrospective study was performed to report the case occurrence of cystic echinococcosis (CE) in three hospitals of north-eastern region in Punjab Province, Pakistan. We reviewed retrospectively the clinical records of patients in 4 hospitals which were diagnosed with CE during 2012-2017. A total of 198 cases, 82 (41.4%) male and 116 (58.6%) females were detected as CE. The most Highest incidence was revealed in.... 21-30 years-old group (24.2%) followed by 41-50 (22.7%), 31-40 (16.2%), 11-20 (13.6%), 51-60 (8.1%), below 10 (5.5%), over 71 (5.1%) and 61-70 year-old group (4.5%). CE was detected in various organs of infected individuals. However, most of CE cases were detected in the liver (47.4%) and lung (18.6%). The present study indicated that CE is more or less prevalent in surveyed areas and one of the most important public health problems in Punjab Province, Pakistan.

Citations

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  • Prevalence and molecular characterization of cystic echinococcosis in livestock in the Hazara Division, Pakistan
    Safia Arbab, Hanif Ullah, Inam Ul Hassan, Suleman, Weiwei Wang, Abdul Qadeer, Jiya Zhang
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • A Retrospective Cohort Study on Human Cystic Echinococcosis in Khyber Pakhtunkhwa Province (Pakistan) Based on 16 Years of Hospital Discharge Records
    Huma Khan, Adriano Casulli, Majid Fasihi Harandi, Muhammad Sohail Afzal, Muhammad Arif Nadeem Saqib, Haroon Ahmed
    Pathogens.2022; 11(2): 194.     CrossRef
  • Design of highly sensitive nano-biosensor for diagnosis of hydatid cyst based on gold nanoparticles
    Fariba Jafari, Amir Hossein Maghsood, Mohammad Fallah, Ahmad Jalilvand, Mohammad Matini, Bahram Amini
    Photodiagnosis and Photodynamic Therapy.2022; 38: 102786.     CrossRef
  • Seropozitifity of Anti-Echinococcus granulosus in Patients with of Clinical Prediagnosis Cystic Echinococcosis at Kafkas University Health Research and Application Hospital
    Mükremin Özkan Arslan, Neriman Mor, Hilal Bedir
    Turkish Journal of Parasitology.2022; 46(2): 129.     CrossRef
  • Investigation of Echinococcus multilocularis in foxes and dogs in Pakistan by detection of copro-DNA
    Aisha Khan, Gérald Umhang, Zaib Ullah, Franck Boué, Vanessa Bastid, Ikram Ullah, Sajid Mahmood, Muhammad Sohail Afzal, Haroon Ahmed
    Parasitology Research.2021; 120(2): 731.     CrossRef
  • Case report of an unexpected inflammatory myofibroblastic tumor
    Vishal Farid Raza, Dawood Arshad, Sajeel Ahmad, Khalid Javeed Khan
    Annals of Medicine & Surgery.2021;[Epub]     CrossRef
  • A review of the diagnosis and management of liver hydatid cyst
    Ester Ferrer-Inaebnit, Francesc Xavier Molina-Romero, Juan José Segura-Sampedro, Xavier González-Argenté, José Miguel Morón Canis
    Revista Española de Enfermedades Digestivas.2021;[Epub]     CrossRef
  • Demographics of cystic echinococcosis patients treated surgically in Lahore, Pakistan: A single centre study from 2007 – 2018
    Q. Rasib, A. Khan, H. Ahmed, S. Nizamuddin, F. Asif, M. S. Afzal, S. Simsek, F. Khurshid, S. Irum, N. Hussain, S. Riaz, S. S. Khan, C. M. Budke
    Helminthologia.2021; 58(2): 162.     CrossRef
  • Echinococcoses in Iran, Turkey, and Pakistan: Old Diseases in the New Millennium
    Mehdi Borhani, Saeid Fathi, Enayat Darabi, Fatemeh Jalousian, Sami Simsek, Haroon Ahmed, Harun Kaya Kesik, Seyed Hossein Hosseini, Thomas Romig, Majid Fasihi Harandi, Iraj Mobedi
    Clinical Microbiology Reviews.2021;[Epub]     CrossRef
  • Clinical and pathogenetic aspects of the complications after surgical treatment of hepatic echinococcosis
    F. G. Nazyrov, A. Kh. Babadjanov, F. R. Yakubov
    Annaly khirurgicheskoy gepatologii = Annals of HPB Surgery.2021; 26(4): 51.     CrossRef
  • Detection of Anti-Echinococcus granulosus Antibodies in Humans: An Update from Pakistan
    Huma Khan, Haroon Ahmed, Muhammad Sohail Afzal, Usman Ayub Awan, Muhammad Khurram, Sami Simsek, Jianping Cao
    Pathogens.2021; 11(1): 29.     CrossRef
  • Cystic echinococcosis: a 10-year experience from a middle-income country
    Ayesha Butt, Javaid Ahmed Khan
    Tropical Doctor.2020; 50(2): 117.     CrossRef
  • First report of Echinococcus canadensis (G6/G7) by sequence analysis from the Khyber Pakhtunkhwa province of Pakistan
    Aisha Khan, Haroon Ahmed, Huma Khan, Sami Simsek, Seyma Gunyakti Kilinc, Harun Kaya Kesik, Guan Yayi, Figen Celik, Muhammad Sohail Afzal, Christine M. Budke
    Acta Tropica.2020; 209: 105559.     CrossRef
  • Human cystic echinococcosis in southwest Iran: a 15-year retrospective epidemiological study of hospitalized cases
    Reza Shahriarirad, Amirhossein Erfani, Mehrdad Eskandarisani, Mohammad Rastegarian, Hajar Taghizadeh, Bahador Sarkari
    Tropical Medicine and Health.2020;[Epub]     CrossRef
  • Cystic Echinococcosis in Pakistan: A Review of Reported Cases, Diagnosis, and Management
    Aisha Khan, Haroon Ahmed, Huma Khan, Sadia Saleem, Sami Simsek, Enrico Brunetti, Muhammad Sohail Afzal, Tommaso Manciulli, Christine M. Budke
    Acta Tropica.2020; 212: 105709.     CrossRef
  • Management Outcome in Simple and Complex Hydatid Cysts of Lung
    Pratikshya Thapaliya, Tanveer Ahmad, Ambreen Abid, Nazish Sikander, Misauq Mazcuri, Nadir Ali
    Cureus.2020;[Epub]     CrossRef
  • Retrospective Study of Cystic Echinococcosis (CE) Based on Hospital Record from Five Major Metropolitan Cities of Pakistan
    Hira Muqaddas, Muhammad Arshad, Haroon Ahmed, Naunain Mehmood, Aisha Khan, Sami Simsek
    Acta Parasitologica.2019; 64(4): 866.     CrossRef
  • 8,476 View
  • 157 Download
  • 16 Web of Science
  • Crossref

Original Article

Epidemiological Aspects of Cutaneous Leishmaniasis during 2009-2016 in Kashan City, Central Iran
Doroodgar Moein, Doroodgar Masoud, Mahboobi Saeed, Doroodgar Abbas
Korean J Parasitol 2018;56(1):21-24.
Published online February 28, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.1.21
Cutaneous leishmaniasis (CL) can be seen in 2 forms, zoonotic and anthroponotic, in Iran. In this study, epidemiological aspects of CL were studied during an 8-year period (2009-2016) in city of Kashan, central Iran. The demographic and epidemiological data, including age, sex, occupation, number and site of the lesions, treatment regimen, past history of CL, and season of all patients were gathered from the health centers. Descriptive statistics were used to describe features of the study data. Total 2,676 people with CL were identified. The highest annual incidence was estimated to be 182 per 100,000 population in 2009 and the least was in 2016 (47 per 100,000 population). The highest frequency affected age groups were observed in 20-29 year-old patients (20.9%). More than 51% of the patients were under 30 years old. The maximum frequency of the disease, 1,134 (43.3%), was seen in autumn. The most common location of lesions was hands (61.4%). Most of the patients (81.6%) were treated by systemic glucantime regimen. In the city of Kashan, the incidence rate of the CL disease is significantly higher than many other regions of Iran. To reduce the risk of disease, control of reservoir hosts and vectors of disease, and education of individual protection are strongly recommended.

Citations

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  • Molecular diagnosis and epidemiological aspects of cutaneous Leishmaniasis in Aleppo: Current status
    Lana Kourieh, Mohammad Y. Abajy, Mahasen Alkebajy, Silva Ishkhanian, Ream Nayal
    Parasite Epidemiology and Control.2025; 28: e00412.     CrossRef
  • Residential environment, human behavior and socio-economic status in transmission of cutaneous leishmaniasis in central Iran
    Asieh Heidari, Nadia Mohammadi Dashtaki, Sima Mizbani, Mehri Rejali, Mohammad Reza Maracy
    Scientific Reports.2025;[Epub]     CrossRef
  • Tracking of Leishmania spp. in Iranian phlebotominae, distribution and biodiversity in Jarqavieh county, central Iran
    Mehdi Haddadnia, Mahsa Esmaeilifallah, Parisima Badiezadeh, Seyed Mohammad Abtahi
    International Journal of Environmental Health Research.2025; : 1.     CrossRef
  • Investigating the Effects of Demographic Factors on the Prevalence of Cutaneous Leishmaniasis in a Focus Area of Northeastern Iran
    Fateme Rajabi Gharaii, Mitra Boroomand, Amin Mohammadpour, Mohadeseh Motamed-Jahromi, Aboozar Soltani, Sumi Mukhopadhyay
    Journal of Tropical Medicine.2025;[Epub]     CrossRef
  • Evaluation of the biodiversity and distribution of sand flies and tracking of Leishmania major in them in central Iran
    Parisima Badiezadeh, Mahsa Esmaeilifallah, Mehdi Haddadniaa, Seyed Mohammad Abtahi
    International Journal of Environmental Health Research.2024; 34(9): 3245.     CrossRef
  • A Retrospective Analysis of Human Cutaneous Leishmaniasis Epidemiology During 2016 - 2021 in Hoveyzeh County, Khuzestan Province, Iran
    Mehdi Kian, Naser Hatamzadeh, Hamid Kassiri, Abas Naboureh, Zahra Makiani, Somayeh Mirzavand
    Jundishapur Journal of Health Sciences.2024;[Epub]     CrossRef
  • Educational WhatsApp-delivered intervention based on social cognitive theory to promote leishmaniosis preventive behavior of health ambassadors: a randomized controlled trial
    Monireh Abdollahi, Mahmoud Fakhar, Mohammad Tajfard, Jamshid Jamali, Mehrsadat Mahdizadeh
    BMC Infectious Diseases.2024;[Epub]     CrossRef
  • Antileishmanial Activity of Tamoxifen by Targeting Sphingolipid Metabolism: A Review
    Kaleab Alemayehu Zewdie, Haftom Gebregergs Hailu, Muluken Altaye Ayza, Bekalu Amare Tesfaye
    Clinical Pharmacology: Advances and Applications.2022; Volume 14: 11.     CrossRef
  • Highly sensitive nested polymerase chain reaction to improve the detection of Leishmania species in clinical specimens
    Hassan Mohammad Tawfeeq, Shahnaz AbdulKader Ali
    Journal of Parasitic Diseases.2022; 46(3): 754.     CrossRef
  • Gender and Cutaneous Leishmaniasis in Israel
    Michal Solomon, Inbal Fuchs, Yael Glazer, Eli Schwartz
    Tropical Medicine and Infectious Disease.2022; 7(8): 179.     CrossRef
  • Cutaneous leishmaniasis in Iran: A review of epidemiological aspects, with emphasis on molecular findings
    Zahra Nasiri, Mohsen Kalantari, Jalal Mohammadi, Salman Daliri, Davood Mehrabani, Kourosh Azizi
    Parasite.2022; 29: 47.     CrossRef
  • Cutaneous Leishmaniasis Based on Climate Regions in Iran (1998-2021)
    Mehri Rejali, Nadia Mohammadi Dashtaki, Afshin Ebrahimi, Asieh Heidari, Mohammad Reza Maracy
    Advanced Biomedical Research.2022; 11(1): 120.     CrossRef
  • Spatiotemporal Variability of Zoonotic Cutaneous Leishmaniasis Based on Sociodemographic Heterogeneity. The Case of Northeastern Iran, 2011–2016
    Mohammad Tabasi, Ali Asghar Alesheikh
    Japanese Journal of Infectious Diseases.2021; 74(1): 7.     CrossRef
  • Recombinant C-Reactive Protein: A Potential Candidate for the Treatment of Cutaneous Leishmaniasis of BALB/c Mice Caused by Leishmania major
    Seyedeh Noushin Zahedi, Seyed Hossein Hejazi, Maryam Boshtam, Farahnaz Amini, Hossein Fazeli, Mahdieh Sarmadi, Mahsa Rahimi, Hossein Khanahmad
    Acta Parasitologica.2021; 66(1): 53.     CrossRef
  • Molecular identification of cutaneous leishmaniasis species using kinetoplast DNA (kDNA) gene in southeast of Iran
    Gholamreza Motalleb, Hamid MalekRaeesi, Hadi Mirahmadi, Amir Tavakoli Kareshk, Rahmat Solgi
    Gene Reports.2021; 22: 101005.     CrossRef
  • Reemergence of zoonotic cutaneous leishmaniasis in an endemic focus, northeastern Iran
    Hasan Jalali, Ahmad Ali Enayati, Mahdi Fakhar, Farzad Motevalli-Haghi, Jamshid Yazdani Charati, Omid Dehghan, Nasibeh Hosseini-Vasoukolaei
    Parasite Epidemiology and Control.2021; 13: e00206.     CrossRef
  • A Ten-Year Trend of Cutaneous Leishmaniasis at University of Gondar Hospital, Northwest Ethiopia: 2009-2018
    Ayalew Jejaw Zeleke, Adane Derso, Arega Yeshanew, Rezika Mohammed, Helina Fikre, Bernard Marchand
    Journal of Parasitology Research.2021; 2021: 1.     CrossRef
  • Vector-borne diseases and tourism in Iran: Current issues and recommendations
    Parisa Soltan-Alinejad, Aboozar Soltani
    Travel Medicine and Infectious Disease.2021; 43: 102108.     CrossRef
  • Androgen-dependent immune modulation in parasitic infection
    Julie Sellau, Marie Groneberg, Hannelore Lotter
    Seminars in Immunopathology.2019; 41(2): 213.     CrossRef
  • Sex-Related Differences in Immune Response and Symptomatic Manifestations to Infection with Leishmania Species
    Ryan D. Lockard, Mary E. Wilson, Nilda E. Rodríguez
    Journal of Immunology Research.2019; 2019: 1.     CrossRef
  • Spatial epidemiology of cutaneous leishmaniasis in Colombia: socioeconomic and demographic factors associated with a growing epidemic
    Andrés M Hernández, Juan D Gutierrez, Yanyu Xiao, Adam J Branscum, Diego F Cuadros
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2019; 113(9): 560.     CrossRef
  • Cutaneous leishmaniasis in Qasr-e Shirin, a border area in the west of Iran
    Yazdan Hamzavi, Naser Nazari, Nahid Khademi, Keivan Hassani, Arezoo Bozorgomid
    Veterinary World.2018; : 1692.     CrossRef
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  • 157 Download
  • 22 Web of Science
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Brief Communication

Epidemiological Investigation of Asymptomatic Dogs with Leishmania Infection in Southwestern China Where Visceral Leishmaniasis is Intractable
Gui-Hua Zhao, Kun Yin, Wei-Xia Zhong, Ting Xiao, Qing-Kuan Wei, Yong Cui, Gong-Zhen Liu, Chao Xu, Hong-Fa Wang
Korean J Parasitol 2016;54(6):797-801.
Published online December 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.6.797
Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.

Citations

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  • Re-emergence of canine Leishmania infantum infection in mountain areas of Beijing
    Gang Liu, Yuanheng Wu, Lei Wang, Yang Liu, Wei Huang, Yifan Li, Mengbo Gao, John Kastelic, Herman Wildrik Barkema, Zhaofei Xia, Yipeng Jin
    One Health Advances.2023;[Epub]     CrossRef
  • Spatio-temporal clustering of Mountain-type Zoonotic Visceral Leishmaniasis in China between 2015 and 2019
    Yuwan Hao, Xiaokang Hu, Yanfeng Gong, Jingbo Xue, Zhengbin Zhou, Yuanyuan Li, Qiang Wang, Yi Zhang, Shizhu Li, Johan Van Weyenbergh
    PLOS Neglected Tropical Diseases.2021; 15(3): e0009152.     CrossRef
  • Epidemiological features and spatial–temporal distribution of visceral leishmaniasis in mainland China: a population-based surveillance study from 2004 to 2019
    Zhou Guan, Can Chen, Chenyang Huang, Hongwei Zhang, Yiyi Zhou, Yuqing Zhou, Jie Wu, Zhengbin Zhou, Shigui Yang, Lanjuan Li
    Parasites & Vectors.2021;[Epub]     CrossRef
  • Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
    Laura Ramírez, Luana Dias de Moura, Natalia Lopes Fontoura Mateus, Milene Hoehr de Moraes, Leopoldo Fabrício Marçal do Nascimento, Nailson de Jesus Melo, Lucas Bezerra Taketa, Tatiana Catecati, Samuel G. Huete, Karla Penichet, Eliane Mattos Piranda, Aless
    Parasite Epidemiology and Control.2020; 8: e00126.     CrossRef
  • Visceral leishmaniasis: a global overview
    Richard G. Wamai, Jorja Kahn, Jamie McGloin, Galen Ziaggi
    Journal of Global Health Science.2020;[Epub]     CrossRef
  • Leishmania infection and blood sources analysis in Phlebotomus chinensis (Diptera: Psychodidae) along extension region of the loess plateau, China
    Han-Ming Chen, Hui-Ying Chen, Feng Tao, Jing-Peng Gao, Kai-Li Li, Hua Shi, Heng Peng, Ya-Jun Ma
    Infectious Diseases of Poverty.2020;[Epub]     CrossRef
  • Visceral leishmaniasis in northwest China from 2004 to 2018: a spatio-temporal analysis
    Canjun Zheng, Liping Wang, Yi Li, Xiao-Nong Zhou
    Infectious Diseases of Poverty.2020;[Epub]     CrossRef
  • Recombinant prohibitin protein of Leishmania infantum acts as a vaccine candidate and diagnostic marker against visceral leishmaniasis
    Daniel S. Dias, Patrícia A.F. Ribeiro, Vívian T. Martins, Daniela P. Lage, Fernanda F. Ramos, Anna L.T. Dias, Marcella R. Rodrigues, Áquila S.B. Portela, Lourena E. Costa, Rachel B. Caligiorne, Bethina T. Steiner, Miguel A. Chávez-Fumagalli, Beatriz C.S.
    Cellular Immunology.2018; 323: 59.     CrossRef
  • Changes in the concentration of anti-Leishmania antibodies in saliva of dogs with clinical leishmaniosis after short-term treatment
    Ana Cantos-Barreda, Damián Escribano, José J. Cerón, Fernando Tecles, Luis J. Bernal, Silvia Martínez-Subiela
    Veterinary Parasitology.2018; 254: 135.     CrossRef
  • Leishmania infantum -specific IFN-γ production in stimulated blood from dogs with clinical leishmaniosis at diagnosis and during treatment
    Pamela Martínez-Orellana, Daniel Marí-Martorell, Sara Montserrat-Sangrà, Laura Ordeix, Gad Baneth, Laia Solano-Gallego
    Veterinary Parasitology.2017; 248: 39.     CrossRef
  • 7,236 View
  • 145 Download
  • 10 Web of Science
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Original Articles

Allelic Diversity and Geographical Distribution of the Gene Encoding Plasmodium falciparum Merozoite Surface Protein-3 in Thailand
Vorthon Sawaswong, Phumin Simpalipan, Napaporn Siripoon, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat
Korean J Parasitol 2015;53(2):177-187.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.177
Merozoite surface proteins (MSPs) of malaria parasites play critical roles during the erythrocyte invasion and so are potential candidates for malaria vaccine development. However, because MSPs are often under strong immune selection, they can exhibit extensive genetic diversity. The gene encoding the merozoite surface protein-3 (MSP-3) of Plasmodium falciparum displays 2 allelic types, K1 and 3D7. In Thailand, the allelic frequency of the P. falciparum msp-3 gene was evaluated in a single P. falciparum population in Tak at the Thailand and Myanmar border. However, no study has yet looked at the extent of genetic diversity of the msp-3 gene in P. falciparum populations in other localities. Here, we genotyped the msp-3 alleles of 63 P. falciparum samples collected from 5 geographical populations along the borders of Thailand with 3 neighboring countries (Myanmar, Laos, and Cambodia). Our study indicated that the K1 and 3D7 alleles coexisted, but at different proportions in different Thai P. falciparum populations. K1 was more prevalent in populations at the Thailand-Myanmar and Thailand-Cambodia borders, whilst 3D7 was more prevalent at the Thailand-Laos border. Global analysis of the msp-3 allele frequencies revealed that proportions of K1 and 3D7 alleles of msp-3 also varied in different continents, suggesting the divergence of malaria parasite populations. In conclusion, the variation in the msp-3 allelic patterns of P. falciparum in Thailand provides fundamental knowledge for inferring the P. falciparum population structure and for the best design of msp-3 based malaria vaccines.

Citations

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  • Genetic diversity of Plasmodium falciparum AMA-1 antigen from the Northeast Indian state of Tripura and comparison with global sequences: implications for vaccine development
    Tulika Nirmolia, Md. Atique Ahmed, Vinayagam Sathishkumar, Nilanju P. Sarma, Dibya R. Bhattacharyya, Pradyumna K. Mohapatra, Devendra Bansal, Praveen K. Bharti, Rakesh Sehgal, Jagadish Mahanta, Ali A. Sultan, Kanwar Narain, Saurav J. Patgiri
    Malaria Journal.2022;[Epub]     CrossRef
  • Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates
    Hương Giang Lê, Thị Lam Thái, Jung-Mi Kang, Jinyoung Lee, Mya Moe, Tuấn Cường Võ, Haung Naw, Moe Kyaw Myint, Zaw Than Htun, Tong-Soo Kim, Ho-Joon Shin, Byoung-Kuk Na
    Malaria Journal.2020;[Epub]     CrossRef
  • Unraveling Haplotype Diversity of the Apical Membrane Antigen-1 Gene in Plasmodium falciparum Populations in Thailand
    Lalita Lumkul, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat
    The Korean Journal of Parasitology.2018; 56(2): 153.     CrossRef
  • Genetic diversity of the merozoite surface protein-3 gene in Plasmodium falciparum populations in Thailand
    Sittiporn Pattaradilokrat, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Napaporn Siripoon, Pongchai Harnyuttanakorn
    Malaria Journal.2016;[Epub]     CrossRef
  • 11,969 View
  • 114 Download
  • 4 Web of Science
  • Crossref
Molecular Variation in the Paragonimus heterotremus Complex in Thailand and Myanmar
Oranuch Sanpool, Pewpan M. Intapan, Tongjit Thanchomnang, Penchom Janwan, Yukifumi Nawa, David Blair, Wanchai Maleewong
Korean J Parasitol 2013;51(6):677-681.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.677

Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Of the 7 members of the genus known in Thailand until recently, only P. heterotremus has been confirmed as causing human disease. An 8th species, P. pseudoheterotremus, has recently been proposed from Thailand, and has been found in humans. Molecular data place this species as a sister species to P. heterotremus, and it is likely that P. pseudoheterotremus is not specifically distinct from P. heterotremus. In this study, we collected metacercariae of both nominal species (identification based on metacercarial morphology) from freshwater crabs from Phetchabun Province in northern Thailand, Saraburi Province in central Thailand, and Surat Thani Province in southern Thailand. In addition, we purchased freshwater crabs imported from Myanmar at Myawaddy Province, western Thailand, close to the Myanmar-Thailand border. The DNAs extracted from excysted metacercariae were PCR-amplified and sequenced for ITS2 and cox1 genes. The ITS2 sequences were nearly identical among all samples (99-100%). Phylogenies inferred from all available partial cox1 sequences contained several clusters. Sequences from Indian P. heterotremus formed a sister group to sequences from P. pseudoheterotremus-type metacercariae. Sequences of P. heterotremus from Thailand, Vietnam, and China formed a separate distinct clade. One metacercaria from Phitsanulok Province was distinct from all others. There is clearly considerable genetic variation in the P. heterotremus complex in Thailand and the form referred to as P. pseudoheterotremus is widely distributed in Thailand and the Thai-Myanmar border region.

Citations

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  • Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic
    Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool
    Infection, Genetics and Evolution.2025; 128: 105718.     CrossRef
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    Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
    Parasitology Research.2024;[Epub]     CrossRef
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    Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang
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Articles from Symposium on Asian Taenia (October 2011, Osong, Korea)

State of the Art of Taenia solium as Compared to Taenia asiatica
Ana Flisser
Korean J Parasitol 2013;51(1):43-49.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.43

Three species of tapeworms infect humans in their adult stage (Taenia solium, Taenia saginata and Taenia asiatica). The 3 are flat, opaque white or yellowish, and exceptional long segmented parasites, measuring 1 to 12 m in their adult stage. In this review, the development of the knowledge regarding the first species, mainly focused on understanding how the larval stage or cysticercus is transmitted to humans, is described. The second species is a cosmopolitan parasite that only causes taeniosis and not cysticercosis; therefore, it will not be included. Information on the third species, which is presently being produced, since this species was recognized as such only at the end of the 20th century, will be discussed at the end of this review.

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Original Article

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock
Korean J Parasitol 2011;49(2):139-144.
Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.139

The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.

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Mini Review

Foodborne Intestinal Flukes in Southeast Asia
Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(Suppl):S69.
Published online October 23, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.S.S69

In Southeast Asia, a total of 59 species of foodborne intestinal flukes have been known to occur in humans. The largest group is the family Heterophyidae, which constitutes 22 species belonging to 9 genera (Centrocestus, Haplorchis, Heterophyes, Heterophyopsis, Metagonimus, Procerovum, Pygidiopsis, Stellantchasmus, and Stictodora). The next is the family Echinostomatidae, which includes 20 species in 8 genera (Artyfechinostomum, Acanthoparyphium, Echinochasmus, Echinoparyphium, Echinostoma, Episthmium, Euparyphium, and Hypoderaeum). The family Plagiorchiidae follows the next containing 5 species in 1 genus (Plagiorchis). The family Lecithodendriidae includes 3 species in 2 genera (Phaneropsolus and Prosthodendrium). In 9 other families, 1 species in 1 genus each is involved; Cathaemaciidae (Cathaemacia), Fasciolidae (Fasciolopsis), Gastrodiscidae (Gastrodiscoides), Gymnophallidae (Gymnophalloides), Microphallidae (Spelotrema), Neodiplostomidae (Neodiplostomum), Paramphistomatidae (Fischoederius), Psilostomidae (Psilorchis), and Strigeidae (Cotylurus). Various types of foods are sources of human infections. They include freshwater fish, brackish water fish, fresh water snails, brackish water snails (including the oyster), amphibians, terrestrial snakes, aquatic insects, and aquatic plants. The reservoir hosts include various species of mammals or birds.The host-parasite relationships have been studied in Metagonimus yokogawai, Echinostoma hortense, Fasciolopsis buski, Neodiplostomum seoulense, and Gymnophalloides seoi; however, the pathogenicity of each parasite species and host mucosal defense mechanisms are yet poorly understood. Clinical aspects of each parasite infection need more clarification. Differential diagnosis by fecal examination is difficult because of morphological similarity of eggs. Praziquantel is effective for most intestinal fluke infections. Continued efforts to understand epidemiological significance of intestinal fluke infections, with detection of further human cases, are required.

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Brief Communications

The incidence and etiology of parasite-associated gastroenteritis during 2004-2006 in Gyeonggi-do (province), South Korea was determined by means of antigen detection ELISA on 6,071 stool specimens collected from 6 general hospitals. At least 1 parasitic agent was detected in 3.4% (208/6,071) of the stool samples. Among these, Giardia lamblia was the most numerous (152 cases; 2.5%), followed by Entamoeba histolytica (25 cases; 0.4%), Cryptosporidium parvum (23 cases; 0.4%), and mixed infections (8 cases; 0.1%). Patients aged 1-5 years had the largest proportion (69.2%; 144/208) of parasite-positive stool specimens. Parasite-mediated gastroenteritis was most common from June to September. The detection rate gradually increased from 2004 to 2006. This study shows that parasite-mediated gastroenteritis may be significant among children in Korea and that parasite infection surveillance should be constantly performed.

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A Serological Study on Toxoplasma gondii Infection Among People in South of Tehran, Iran
Abdoreza Salahi-Moghaddam, Abbas Hafizi
Korean J Parasitol 2009;47(1):61-63.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.61

Although Toxoplasma gondii infection generally shows subclinical courses in adults, it may have severe pathologic effects on the fetus. For a survey of toxoplasmosis in Shahr-e-Rey area, south of Tehran, Iran, we conducted a serological study on 1,187 general populations using indirect fluorescent antibody (IFA) test. In this cross sectional study, cases were selected randomly by a local primary health care (PHC) system. Blood samples were collected in microcapillary tubes and sera were examined by IFA after centrifugation. It has been shown that 68.3% of cases in all age groups had IFA titer of 1 : 10 and higher, which we consider as positive cases, and the rate for 1 : 400 or over was 30.2%. There was meaningful correlation between anti-Toxoplasma antibody titers and age, sex, and occupation of subjected people (P < 0.05%). The incidence was higher in females and highest in the children age group (10-14 years old). The present study demonstrates high prevalence of Toxoplasma infection among the people in south of Tehran, Iran.

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Original Articles

A seroepidemiological survey of Taenia solium cysticercosis in Nabo, Guangxi Zhuang Autonomous Region, China
Joon-Yong Chung, Keeseon S. Eom, Yichao Yang, Xenming Li, Zheng Feng, Han-Jong Rim, Seung-Yull Cho, Yoon Kong
Korean J Parasitol 2005;43(4):135-139.
Published online December 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.4.135

We have observed the seropositive rate of Taenia solium cysticercosis in residents at Nabo Village, Tiandong County, Guangxi Zhuang Autonomous Region, China by enzyme linked immunosorbent assay. The village had been found to be a relatively high endemic area of porcine cysticercosis among roaming pigs. Of 202 persons examined four males aged 15, 25, 35 and 41 year-old exhibited absorbance (abs) at 0.18, 0.20, 0.35 and 0.55, respectively. In addition, two females whose ages were 35 and 39 years revealed specific antibody levels of abs 0.26 and 0.41 in their sera. Overall positive rate among the people was 2.97%. All of these persons agreed that they had ingested the pork infected with T. solium metacestode (TsM), while history of proglottid discharge was not noticed from all of them. Three males and one female complained of intermittent headache. Our findings reinforced not only that the prevalence of cysticercosis might be related with roaming pigs infected with TsM but also that behavioral and environmental practices in local community constituted risk factors for transmission of the infection.

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Epidemiological survey of Giardia spp. and Blastocystis hominis in an Argentinian rural community
Marta Cecilia Minvielle, Betina Cecilia Pezzani, Mar?a Alejandra Cordoba, Mar?a Marta De Luca, Mar?a Carmen Apezteguia, Juan Angel Basualdo
Korean J Parasitol 2004;42(3):121-127.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.121

The aim of this study was to relate personal data, socio-cultural and environmental characteristics, and the presence of symptoms/signs with the frequencies of Giardia spp. and Blastocystis hominis among a rural population in Buenos Aires Province, Argentina. Of the surveyed population (350), 3.7% were infected with only Giardia spp. or 22.9% with B. hominis, and 2.3% were infected with both protozoa. The frequency of infection according to sex; 6.1% of males were infected and 1.6% of females by Giardia spp., 26.7% and 19.5% by B. hominis, and 2.4% and 2.2% by both parasites, respectively. Giardia spp. was detected in only three adults (over 14 years), but B. hominis was more frequent in adults than in children. The prevalences of these protozoa in this community are lower than those reported by other Argentinean studies, which is probably associated with the low density of the studied population (5.95 inhab/km2). Statistical analysis revealed that a male sex, flooding of the home, the use of a latrine, and an abdominal pain were correlated with the presence of these parasites, which indicate the importance of these factors in rural communities.

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A seroepidemiologic survey for human sparganosis in Gangweon-do
Kyu-Jae Lee, Yong-Tae Bae, Dong-Heui Kim, Young-Kun Deung, Yong-Suk Ryang
Korean J Parasitol 2002;40(4):177-180.
Published online December 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.4.177

Gangweon-do is known to be highly endemic area of sparganosis more than other provinces in Korea. A seroepidmiologic examination for the detection of anti-Spirometra erinacei plerocercoid IgG in serum was carried out in normal inhabitants in Hongcheon-gun, Gangweon-do. Sere were tested by enzyme-linked immunosrobent assay (ELISA) for the anti-sparganum antibodies. Positive rate for anti-sparganum antibody in 719 adults was 3.3%. Data of the questionnaire for 24 ELISA positive inhabitants revealed that 20 had a history of eating raw meat of snakes, 24 had a history of eating frogs, and 24 had a history of drinking stream water. Two positive cases had a past history of sparganosis. Two positive cases showed current symptoms of sparganosis. The data revealed that ELISA would be useful to find infected cases among normal inhabitants at sparganosis-endemic areas.

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    Jeong-Geun Kim, Chun-Seob Ahn, Woon-Mok Sohn, Yukifumi Nawa, Yoon Kong
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Epidemiological study of clonorchiasis and metagonimiasis along the Geum-gang (River) in Okcheon-gun (County), Korea
Gye-Sung Lee, In-Sung Cho, Young-Ha Lee, Hyung-Jun Noh, Dae-Whan Shin, Sok-Goo Lee, Tae-Yong Lee
Korean J Parasitol 2002;40(1):9-16.
Published online March 31, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.1.9

The endemic status of clonorchiasis and metagonimiasis along the Geum-gang (River) in Okcheon-gun (County) in Korea was examined. From February to December 2000, stools of total 1,081 inhabitants living in 5 villages were examined. Each stool specimen was examined by both the cellophane thick smear method and the formalin-ether sedimentation technique. Egg-positive cases were further analyzed by Stoll's egg-counting technique, and praziquantel was administered to positive cases. The egg-positive rates for Clonorchis sinensis and Metagonimus species were 9.3% and 5.5%, respectively, and the double infection rate was 3.5%. The numbers of eggs per gram (EPG) of feces of C. sinensis and Metagonimus sp. were 918±1,463 and 711±947, respectively. The egg-positive rates for C. sinensis and Metagonimus sp. in the riverside area were 14.2% and 8.4%, respectively, which were significantly higher than those of the inland area (3.2% and 1.7%, respectively). The egg-positive rates of C. sinensis and Metagonimus sp. in males (16.7% and 10.0%) were significantly higher than those of females (3.5% and 1.8%). However, there were no significant differences of EPG values between localities and sexes. The prevalence of clonorchiasis and metagonimiasis in this survey was significantly lower than that in the previous reports. However, there is still a high prevalence of infection with C. sinensis and Metagonimus sp. in this region, especially in the riverside area.

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Incidence patterns of vivax malaria in civilians residing in a high-risk county of Kyonggi-do (Province), Republic of Korea
Jung Ju Moon, Seung-Yull Cho
Korean J Parasitol 2001;39(4):293-299.
Published online December 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.4.293

The characteristics of vivax malaria epidemics along the demilitarized zone (DMZ) in the Republic of Korea has been established by the early surveillance data. To further characterize the epidemic, data of civilian patients microscopically diagnosed with malaria from 1995 through 2000 were analyzed in Yonchon-gun (county). Malaria incidence was greater in male civilians > 30 years of age (p < 0.05). The annual parasite index was significantly higher in those living in the administrative areas (Myeon) traversed by DMZ than those living in Myons not traversed by DMZ (p < 0.05). Analysis according to the distance (4 to 14 km) from DMZ showed that people living in villages close to DMZ had higher annual parasite indices than those living in villages remote from DMZ (p for trend < 0.05). Civilians living in Myeons with plains and located in northwestern part of the county had higher annual parasite indices than those living in hilly Myeons located in southeastern part of the county (p for trend < 0.05). These findings suggest that the contraction of vivax malaria is related with night-time outdoor activities, and that the distance from DMZ is a risk factor. In this area, the flying distance of infected vector mosquitos can explain the annually repeating occurrence of civilian cases.

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Epidemiologic characteristics of malaria in non-malarious area, Jeollabuk-do, Korea in 2000
Myung-Bin Kim
Korean J Parasitol 2001;39(3):223-226.
Published online September 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.3.223

In South Korea, the north border area has been under vivax malaria epidemic since 1993. However, Jeollabuk-do, which is about 300 kms from the border, has not experienced the same epidemic. I investigated a total of 58 notified cases of malaria in Jeollabuk-do in the year 2000. All of the cases had an exposure history in the epidemic area. Among them were 49 ex-soldiers, 3 soldiers who served near the border area and 6 civilians who traveled there. The causal agent of all cases was Plasmodium vivax. Except the civilians, the soldiers and ex-soldiers were aged in their twenty's. In the present study, the incubation period was from 6 to 520 days with a median of 157 days, and the latent onset type (92%) was more prevalent than the early onset type. Illness onset of most cases (86%) peaked during the summer season (June to September) despite of variable incubation periods. The time lag for diagnosis ranged from 2 to 42 days with a median of 11 days. Jeollabuk-do has not been an area of epidemic untill now, but incidences have been increasing annually since 1996. In Jeollabuk-do, early diagnosis and treatment can be a feasible disease control measure to prevent spreading from the epidemic area.

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Seroepidemiological study of Toxoplasma gondii infection in the rural area Okcheon-gun, Korea
Young-Ha Lee, Hyung-Jun Noh, Ok-Sun Hwang, Sang-Keol Lee, Dae-Whan Shin
Korean J Parasitol 2000;38(4):251-256.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.251

There have been some reports about the prevalence of anti-Toxoplasma gondii antibody among Koreans, and most of all data were taken from patients visiting hospitals. However, the epidemiological data of the community-based study in Korea are rare. This study was performed to evaluate the seroprevalence of toxoplasmosis among the inhabitants of the rural area Okcheon-gun, Korea. A total of 1,109 serum samples (499 males, 610 females) were examined for the IgG antibodies by ELISA. To set up the cut-off point for ELISA, we used a commercial latex agglutination (LA) kit. The sensitivity and specificity of ELISA against LA test were 89.5%, and 98.6% respectively. Among 1,109 sera, 6.9% showed seropositivity by ELISA. The positive rates of males and females were 6.0% and 7.2%, respectively. However, there were no significant differences between sexes. Comparing the age groups, the highest seropositive rate showed in the seventies or higher, and their rates had a tendency to increase with age (0.05 < p < 0.3). These results revealed that the seroprevalence of toxoplasmosis in rural inhabitants is similar to previous reports in Korea; however we need further investigation to clarify the prevalence of toxoplasmosis in the general population.

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  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
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Brief Communications

Intestinal parasite infections at an institution for the handicapped in Korea
Jongweon Lee, Gab-Man Park, Du-Ho Lee, Soon-Jung Park, Tai-Soon Yong
Korean J Parasitol 2000;38(3):179-181.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.179

Stool and cellotape anal swab examinations were carried out in August 1997 on handicapped people at an institution located in Chorwon-gun, Kangwon-do, Korea. A total of 112 stool samples (78 males and 34 females) revealed three cases of Trichuris trichiura infection and one case of Enterobius vermicularis infection. Other helminth eggs were not detected. The overall prevalence rate was 35.7% (38.5% for males and 29.4% for females). More than two different kinds of parasites were found in 42.0% of the positive stool samples (17 cases). The infection rates for protozoan cysts are as follow: Entamoeba coli (25.0%), E. histolytica (1.8%), Endolimax nana (21.4%), Iodoamoeba b?tschlii (1.8%) and Giardia lamblia (0.9%). In cellotape anal swab examinations (165 samples), the prevalence rate of E. vermicularis was 20.6% (25.7% of males and 9.6% of females). In conclusion, the handicapped people in the institution showed higher infection rates of protozoan parasites and E. vermicularis, possibly due to more accessibility to the infection.

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    Sung-Tae Hong, Tai-Soon Yong
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Enterobius vermicularis egg positive rate in a primary school in Chungchongnam-do (Province) in Korea
Kyu-Jae Lee, In-Yong Lee, Kyung-il Im
Korean J Parasitol 2000;38(3):177-178.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.177

The egg positive rate of Enterobius vermicularis was investigated among students of a primary school and a kindergarten located in the rural area of Tangjin-gun, Chungchongnam-do in December, 1998. Of the 189 examinees, 28 (14.8%) were found to be infected with E. vermicularis by the adhesive cellotape anal swab method. The infection rates ranged from 4.2% to 26.1% among school children, and the highest rate was observed in children attending kindergarten. Three months after treatment with albendazole, four (14.3%) out of 28 infected children still remained infected with E. vermicularis. Through this survey, we were able to determine that E. vermicularis infection is still prevalent among children in rural areas of Korea.

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    Eman H. Radwan, Amel Abd El Rahman Hassan, Wael M. Lotfy, Ahmed Abd El-Mawgood, Hala M. Mashaal, Patricio De los RÃos
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The first human case of Trichinella spiralis infection in Korea
Woon-Mok Sohn, Han-Mo Kim, Dong-Il Chung, Sung Tae Yee
Korean J Parasitol 2000;38(2):111-115.
Published online June 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.2.111

Three cases of human infection by Trichinella spiralis were first confirmed by detecting encysted larvae in the biopsied muscle in December 1997, in Korea. The patients were one 35- and two 39-year-old males residing in Kochang-gun, Kyongsangnam-do. They had a common past history of eating raw liver, spleen, blood and muscle of a badger, Meles meles melanogenys, and complained of high fever, facial and periorbital edema, and myalgia. Hematologic and biochemical examinations revealed leukocytosis and eosinophilia, and highly elevated levels of GOT, GPT, LDH and CPK. In the gastrocnemius muscle of a patient, roundly coiled nematode larvae were detected. The larvae measured 0.775-1.050 (av. 0.908) mm in length, and 0.026-0.042 (av. 0.035) mm in maximum width. The specific IgG antibody levels in three patients' sera were significantly higher when compared with those of normal controls. The patients were treated with flubendazole and albendazole for 15-30 days, and discharged at 13-34 days post-admission. From the above findings, it was confirmed that T. spiralis is present in Korea, and the badger plays a role of as the natural host.

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High endemicity of Metagonimus yokogawai infection among residents of Samchok-shi, Kangwon-do
Jong-Yil Chai, Eun-Taek Han, Yun-Kyu Park, Sang-Mee Guk, Jae-Lip Kim, Soon-Hyung Lee
Korean J Parasitol 2000;38(1):33-36.
Published online March 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.1.33

A small-scale epidemiological survey was undertaken during 1997-1998 on the residents along the Osib-chon (Stream), Samchok-shi (City), Kangwon-do (Province), to evaluate the status of Metagonimus yokogawai infection. A total of 165 fecal samples was collected and examined by cellophane thick smear and formalin-ether sedimentation techniques. The egg positive rate of M. yokogawai was 29.7%, showing a remarkable difference between males (46.6%) and females (16.3%). To obtain the adult flukes of M. yokogawai, 11 egg positive persons were treated with praziquantel and purged with magnesium sulfate. A total of 242,119 adult flukes (average 22,010 per person, 367-119,650 in range) was collected from diarrheic stools, all of which were identified as M. yokogawai. The results show that M. yokogawai is still highly endemic in this area.

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Original Articles
Toxoplasma gondii antibody titers in sera of children admitted to the Seoul National University Children's Hospital
Jina Kook, Hong-Jin Lee, Beyong Il Kim, Chong-Ku Yun, Sang-Mee Guk, Min Seo, Yun-Kyu Park, Sung-Tae Hong, Jong-Yil Chai
Korean J Parasitol 1999;37(1):27-32.
Published online March 20, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.1.27

A total of 542 children under 10 years of age, admitted to the Seoul National University Children's Hospital, was examined for antibody titers of Toxoplasma gondii using indirect latex agglutination (ILA) test. Among them, 7.7% showed positive titers higher than 1:32, without significant difference between males (7.3%) and females (8.5%). The seropositive rate increased with age although the statistical significance was negligible (0.05<P<0.1). By residential areas, the prevalence appeared higher among children from southern provinces (Kyongsang-do and Cholla-do) than those from other areas, but the statistical significance was also very low (0.05<P<0.1). When the seropositive cases were analyzed by coincidental diseases, the prevalence was significantly higher in patients with congenital diseases than in patients with non-congenital diseases (P<0.05). The results showed that the seropositive rate of toxoplasmosis in children examined was not high compared with other endemic countries. Some correlations are suggested between toxoplasmosis and congenital anomalies in Korea.

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Current status of vivax malaria among civilians in Korea
Jong-Soo Lee, Weon-Gyu Kho, Hyeong-Woo Lee, Min Seo, Won-Ja Lee
Korean J Parasitol 1998;36(4):241-248.
Published online December 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.4.241

A result of national malaria surveillance in Korean civilians was described. Since a case of indigenous vivax malaria was detected in 1993, a total of 2,198 cases was confirmed by blood smear up to 1997. Of them, 1,548 cases were soldiers serving in the demilitarized zone (DMZ), while 650 cases were civilians. Number of civilian cases was 3 in 1994, 19 in 1995, 71 in 1996, and 557 in 1997. Of them, 239 were ex-soldiers who discharged after military service in the prevalent areas such as Paju, Yonchon, Kimpo, Kangwha, Tongduchon in Kyonggi-do and Chorwon in Kangwon-do while 308 patients were civilian residents in the prevalent areas. Seventy-two patients, living nationwide, had a history of visiting the prevalent areas during transmission season. Only 32 civilian patients denied any relation with the prevalent areas. As a whole, a half of the civilian cases was diagnosed when living in non-prevalent areas. Male patients in their twenties was the highest in number. Annual parasite index is steadily elevated in residents living in the prevalent areas. Monthly incidence showed an unimodal distribution, forming a peak in August. Ex-soldiers exhibited a delayed incubation ranging from 153 to 452 days (279 ± 41 days). The time required for diagnosis was shortened from 23.6 days in 1995 to 13.7 days in 1997. Although the current epidemic of vivax malaria started as a border malaria, it seems highly probable that vivax malaria is established in the local areas and responsible for at least a part of transmission.

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