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"Cryptosporidium parvum"

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"Cryptosporidium parvum"

Case Report

A Waterborne Outbreak and Detection of Cryptosporidium Oocysts in Drinking Water of an Older High-Rise Apartment Complex in Seoul
Eun-Joo Cho, Jin-Young Yang, Eun-Sook Lee, Se-Chul Kim, So-Yang Cha, Sung-Tek Kim, Man-Ho Lee, Sun-Hee Han, Young-Sang Park
Korean J Parasitol 2013;51(4):461-466.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.461

From May to June 2012, a waterborne outbreak of 124 cases of cryptosporidiosis occurred in the plumbing systems of an older high-rise apartment complex in Seoul, Republic of Korea. The residents of this apartment complex had symptoms of watery diarrhea and vomiting. Tap water samples in the apartment complex and its adjacent buildings were collected and tested for 57 parameters under the Korean Drinking Water Standards and for additional 11 microbiological parameters. The microbiological parameters included total colony counts, Clostridium perfringens, Enterococcus, fecal streptococcus, Salmonella, Shigella, Pseudomonas aeruginosa, Cryptosporidium oocysts, Giardia cysts, total culturable viruses, and Norovirus. While the tap water samples of the adjacent buildings complied with the Korean Drinking Water Standards for all parameters, fecal bacteria and Cryptosporidium oocysts were detected in the tap water samples of the outbreak apartment complex. It turned out that the agent of the disease was Cryptosporidium parvum. The drinking water was polluted with sewage from a septic tank in the apartment complex. To remove C. parvum oocysts, we conducted physical processes of cleaning the water storage tanks, flushing the indoor pipes, and replacing old pipes with new ones. Finally we restored the clean drinking water to the apartment complex after identification of no oocysts.

Citations

Citations to this article as recorded by  Crossref logo
  • Disruptive effects of sewage intrusion into drinking water: Microbial succession and organic transformation at molecular level
    Mengqing Fan, Anran Ren, Mingchen Yao, Xiaoming Li, Walter van der Meer, Guo Yu, Gertjan Medema, Joan Rose, Gang Liu
    Water Research.2024; 266: 122281.     CrossRef
  • Current status of intestinal parasite infections in fecal samples of dogs in Korea
    You-Jeong Lee, Beoul Kim, Dongmi Kwak, Min-Goo Seo
    Parasites, Hosts and Diseases.2024; 62(4): 438.     CrossRef
  • Molecular identification and antiprotozoal activity of silver nanoparticles on viability of Cryptosporidium parvum isolated from pigeons, pigeon fanciers and water
    Rasha M. M. Abou Elez, Amira S. A. Attia, Hala M. N. Tolba, Reham G. A. Anter, Ibrahim Elsohaby
    Scientific Reports.2023;[Epub]     CrossRef
  • Outbreak of severe diarrhea due to zoonotic Cryptosporidium parvum and C. xiaoi in goat kids in Chungcheongbuk-do, Korea
    Ah-Young Kim, Badriah Alkathiri, Subin Lee, Kyung-Duk Min, Soochong Kim, Sang-Myeong Lee, Wan-Kyu Lee, Dongmi Kwak, Seung-Hun Lee
    Parasitology Research.2023; 122(9): 2045.     CrossRef
  • Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking water treatment residues: comparison of recovery methods for quantity assessment
    Kamila Jessie Sammarro Silva, Lyda Patricia Sabogal-Paz
    Environmental Technology.2021; 42(20): 3144.     CrossRef
  • Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations
    Fernando César Andreoli, Lyda Patricia Sabogal-Paz
    Parasitology Research.2021; 120(2): 629.     CrossRef
  • Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
    Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
    The Korean Journal of Parasitology.2019; 57(5): 531.     CrossRef
  • Monitoring of Noxious Protozoa for Management of Natural Water Resources
    Young Yil Bahk, Pyo Yun Cho, Sung Kyu Ahn, Sangjung Park, Won Hwa Jheong, Yun-Kyu Park, Ho-Joon Shin, Sang-Seob Lee, Okjae Rhee, Tong-Soo Kim
    The Korean Journal of Parasitology.2018; 56(2): 205.     CrossRef
  • 9,538 View
  • 119 Download
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Brief Communication

Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Hyeng-Il Cheun, Kyungjin Kim, Sejoung Yoon, Won-Ja Lee, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu
Korean J Parasitol 2013;51(3):353-355.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.353

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.

Citations

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  • Causes of acute gastroenteritis in Korean children between 2004 and 2019
    Eell Ryoo
    Clinical and Experimental Pediatrics.2021; 64(6): 260.     CrossRef
  • Cryptosporidium spp. Diagnosis and Research in the 21st Century
    Jennifer K. O'Leary, Roy D. Sleator, Brigid Lucey
    Food and Waterborne Parasitology.2021; 24: e00131.     CrossRef
  • Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples
    Felix Weinreich, Andreas Hahn, Kirsten Alexandra Eberhardt, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt
    Pathogens.2021; 10(9): 1131.     CrossRef
  • Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofCryptosporidiumSpecies in Kenyan Children Presenting with Diarrhea
    Timothy S. Mamba, Cecilia K. Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K. Njiru
    Journal of Tropical Medicine.2018; 2018: 1.     CrossRef
  • Expression and Purification of gp40/15 Antigen of Cryptosporidium parvum Parasite in Escherichia coli: an Innovative Approach in Vaccine Production
    Hossein Sobati, Habib Jasor-Gharebagh, Hossein Honari
    Iranian Red Crescent Medical Journal.2017;[Epub]     CrossRef
  • Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
    Semie Hong, Kyungjin Kim, Sejoung Yoon, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu
    Journal of Korean Medical Science.2014; 29(10): 1367.     CrossRef
  • 10,251 View
  • 145 Download
  • Crossref

Original Article

Opportunistic Parasites among Immunosuppressed Children in Minia District, Egypt
Ekhlas H. Abdel-Hafeez, Azza K. Ahmad, Basma A. Ali, Fadia A. Moslam
Korean J Parasitol 2012;50(1):57-62.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.57

A total of 450 stool samples were collected from inpatient and outpatient clinics of Pediatric Department, Minia University Hospital, Minia District, Egypt. Two groups of patients were studied, including 200 immunosuppressed and 250 immunocompetent children. Stool samples were subjected to wet saline and iodine mounts. A concentration technique (formol-ether sedimentation method) was carried out for stool samples diagnosed negative by wet saline and iodine mounts. Samples were stained by 2 different methods; acid fast stain (modified Ziehl-Neelsen stain) and Giemsa stain. Total 188 cases (94%) were diagnosed positive for parasitic infections among immunosuppressed children, whereas 150 cases (60%) were positive in immunocompetent children (P<0.0001). The most common protozoan infection in immunosuppressed group was Cryptosporidium parvum (60.2%), followed by Blastocystis hominis (12.1%), Isospora belli (9.7%), and Cyclospora caytenensis (7.8%). On the other hand, Entamoeba histolytica (24.6%) and Giardia lamblia (17.6%) were more common than other protozoans in immunocompetent children.

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    Sevinç Arzu Postacı, Ali Dal
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    Oliwia Obuch-Woszczatyńska, Klaudia Bylińska, Małgorzata Krzyżowska, Karol Korzekwa, Piotr Bąska
    Pathogens.2025; 14(1): 86.     CrossRef
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    BMC Veterinary Research.2025;[Epub]     CrossRef
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    Hena Butta, Raman Sardana, Anupam Sibal, Leena Mendiratta, Ghanta Naga Sai Snigdha, Smita Malhotra, Yaja Jebaying
    Indian Journal of Medical Microbiology.2025; 58: 100956.     CrossRef
  • The impact of L-citrulline on murine intestinal cell integrity, immune response, and arginine metabolism in the face of Giardia lamblia infection
    Hager S. Zoghroban, Fatma MKh Ibrahim, Nahla A. Nasef, Abeer E. Saad
    Acta Tropica.2023; 237: 106748.     CrossRef
  • Final destination: The Mediterranean Sea, a vulnerable sea. The long journey of Giardia duodenalis cysts
    Federica Berrilli, Roberto Amerigo Papini, Alessandra Barlaam, Giovanni Normanno, Antonella Puccini, Isabel Guadano Procesi, Annunziata Giangaspero
    Environmental Advances.2023; 13: 100414.     CrossRef
  • Cryptosporidiosis is predominantly an urban, anthroponotic infectious disease among Zambian children
    Barbara Banda, Joyce Siwila, Andrew N Mukubesa, Simbarashe Chitanga, Patrick Kaonga, Katendi Changula, Edgar Simulundu, Ngonda Saasa, Paul Kelly
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2022; 116(3): 270.     CrossRef
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    Jad Nasrallah, Mohammad Akhoundi, Djamal Haouchine, Anthony Marteau, Stéphane Mantelet, Philippe Wind, Robert Benamouzig, Olivier Bouchaud, Robin Dhote, Arezki Izri
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    Roya Salehi Kahish, Arash Alghasi, Shekoufe Hadadi, Marziyeh Abasi Nasab, Aida Mafakherzadeh
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    HIV/AIDS - Research and Palliative Care.2021; Volume 13: 81.     CrossRef
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    Andernice dos Santos Zanetti, Antonio Francisco Malheiros, Tatiane Amorim de Matos, Carolina dos Santos, Paula Franciene Battaglini, Luciana Melhorança Moreira, Larissa Maria Scalon Lemos, Solange Kimie Ikeda Castrillon, Denise da Costa Boamorte Cortela,
    Parasite.2021; 28: 17.     CrossRef
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    Antibiotics.2021; 10(5): 477.     CrossRef
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  • Clinical and Laboratory Diagnosis of Cryptosporidiosis among Children with Acute Gastroenteritis at a Tertiary Hospital, Cairo, Egypt
    Mohammad Ashraf Abdel Wahed, Yasmine Elsayed Abdelrahman Shehab, Hanan Mahmoud Abou-Seri, Yosra Mohamed Mohsen Awad
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Brief Communication

In the genus Cryptosporidium, there are more than 14 species with different sizes and habitats, as well as different hosts. Among these, C. parvum and C. hominis are known to be human pathogens. As C. parvum can survive exposure to harsh environmental conditions, including various disinfectants or high doses of radiation, it is considered to be an important environmental pathogen that may be a threat to human health. However, the resistance of other Cryptosporidium species to various environmental conditions is unknown. In this study, resistance against γ-irradiation was compared between C. parvum and C. muris using in vivo infection in mice. The capability of C. muris to infect mice could be eliminated with 1,000 Gy of γ-irradiation, while C. parvum remained infective in mice after up to 1,000 Gy of γ-irradiation, although the peak number of oocysts per gram of feces decreased to 16% that of non-irradiated oocysts. The difference in radioresistance between these 2 Cryptosporidium species should be investigated by further studies.

Citations

Citations to this article as recorded by  Crossref logo
  • Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii
    Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert
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  • 8,244 View
  • 62 Download
  • Crossref

Original Articles

Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation
Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2011;49(1):25-31.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.25

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.

Citations

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    Sandrine Boivin, Hiroshi Nagaoka, Takahiro Fujioka
    Water Research X.2025; 27: 100345.     CrossRef
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    Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert
    Parasitology Research.2023; 122(8): 1819.     CrossRef
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    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
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    Takahiro Sekikawa, Kosuke Toshiki
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  • 92 Download
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Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea
Hyeng-Il Cheun, Shin-Hyeong Cho, Jin-Hee Lee, Yi-Young Lim, Ji-Hye Jeon, Jae-Ran Yu, Tong-Soo Kim, Won-Ja Lee, Seung-Hak Cho, Deog-Yong Lee, Mi-Seon Park, Hye-Sook Jeong, Doo-Sung Chen, Yeong-Mi Ji, Mi-Hwa Kwon
Korean J Parasitol 2010;48(2):113-120.
Published online June 17, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.2.113

To understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixed-infection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the Republic of Korea. A total of 76,652 stool samples were collected from 96 hospitals across the nation. The positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. Especially, Cryptosporidium parvum was highly mixed-infected with rotavirus among pediatric diarrheal patients (29.5 per 100 C. parvum positive cases), and Entamoeba histolytica was mixed-infected with Clostridium perfringens (10.3 per 100 E. histolytica positive cases) in protozoan-diarrheal patients. Those infected with rotavirus and C. perfringens constituted relatively high proportions among mixed infection cases from January to April. The positivity for rotavirus among viral infection for those aged ≤ 5 years was significantly higher, while C. perfringens among bacterial infection was higher for ≥ 50 years. The information for association of viral and bacterial infections with enteropathogenic protozoa in diarrheal patients may contribute to improvement of care for diarrhea as well as development of control strategies for diarrheal diseases in Korea.

Citations

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Brief Communications

The incidence and etiology of parasite-associated gastroenteritis during 2004-2006 in Gyeonggi-do (province), South Korea was determined by means of antigen detection ELISA on 6,071 stool specimens collected from 6 general hospitals. At least 1 parasitic agent was detected in 3.4% (208/6,071) of the stool samples. Among these, Giardia lamblia was the most numerous (152 cases; 2.5%), followed by Entamoeba histolytica (25 cases; 0.4%), Cryptosporidium parvum (23 cases; 0.4%), and mixed infections (8 cases; 0.1%). Patients aged 1-5 years had the largest proportion (69.2%; 144/208) of parasite-positive stool specimens. Parasite-mediated gastroenteritis was most common from June to September. The detection rate gradually increased from 2004 to 2006. This study shows that parasite-mediated gastroenteritis may be significant among children in Korea and that parasite infection surveillance should be constantly performed.

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Comparative Sensitivity of PCR Primer Sets for Detection of Cryptosporidium parvum
Jae-Ran Yu, Soo-Ung Lee, Woo-Yoon Park
Korean J Parasitol 2009;47(3):293-297.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.293

Improved methods for detection of Cryptosporidium oocysts in environmental and clinical samples are urgently needed to improve detection of cryptosporidiosis. We compared the sensitivity of 7 PCR primer sets for detection of Cryptosporidium parvum. Each target gene was amplified by PCR or nested PCR with serially diluted DNA extracted from purified C. parvum oocysts. The target genes included Cryptosporidium oocyst wall protein (COWP), small subunit ribosomal RNA (SSU rRNA), and random amplified polymorphic DNA. The detection limit of the PCR method ranged from 103 to 104 oocysts, and the nested PCR method was able to detect 100 to 102 oocysts. A second-round amplification of target genes showed that the nested primer set specific for the COWP gene proved to be the most sensitive one compared to the other primer sets tested in this study and would therefore be useful for the detection of C. parvum.

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Original Article

A Hospital-Based Serological Survey of Cryptosporidiosis in the Republic of Korea
Jong-Kyu Lee, Eun-Taek Han, Sun Huh, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2009;47(3):219-225.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.219

The seroprevalence of cryptosporidiosis was examined using patients' sera collected from hospitals located in 4 different areas of the Republic of Korea. ELISA was used to measure antibody titers against Cryptosporidium parvum antigens from a total of 2,394 serum samples, which were collected randomly from patients in local hospitals; 1) Chungbuk National University Hospital, 2) Konkuk University Hospital, 3) local hospitals in Chuncheon, Gangwon-do (province), 4) Jeonnam National University Hospital, from 2002 through 2003. Of the 2,394 samples assayed, 34%, 26%, and 56% were positive for C. parvum-specific IgG, IgM, and IgA antibodies, respectively. Positive IgG titers were most common in sera from Jeonnam National University Hospital, Gwangju, Jeollanam-do, and positive IgM titers were most common in sera from Chungbuk National University Hospital, Cheongju, Chuncheongbuk-do. The seropositivity was positively correlated with age for both the IgG and IgA antibodies but was negatively correlated with age for the IgM antibodies. Western blotting revealed that 92%, 83%, and 77% of sera positive for IgG, IgM, and IgA ELISA reacted with 27-kDa antigens, respectively. These results suggested that infection with Cryptosporidium in hospital patients occurs more commonly than previously reported in the Republic of Korea.

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Brief Communication

Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera
Jong-Gyu Lee, Eun-Taek Han, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2009;47(2):171-174.
Published online May 27, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.2.171

The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at various developmental stages of parasites, including asexual, sexual stages, and oocysts. The amount of antigen-stimulating IgG antibodies was particularly high on the oocyst wall. The sporozoite surface was shown to give stimulation on IgG and IgM antibody formation. Trophozoites implicated the lowest antigenicity to humoral immunity, both IgG and IgM, by showing the least amount of gold labeling. Immunogold labeling also provided clues that antigens were presented to the host-cell cytoplasm via feeder organelles and host-parasite junctions.

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  • Cryptostatin, a chagasin-family cysteine protease inhibitor ofCryptosporidium parvum
    J.-M. KANG, H.-L. JU, J.-R. YU, W.-M. SOHN, B.-K. NA
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Original Articles

Quantitative Evaluation of Infectivity Change of Cryptosporidium parvum after Gamma Irradiation
Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2009;47(1):7-11.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.7

Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C. parvum after gamma irradiation and repair of the infectivity during incubation time after irradiation. C. parvum oocysts were subjected to gamma irradiation at various doses (1, 5, 10, and 25 kGy), and the in vitro infectivity was measured by real-time PCR every day up to 7 days after irradiation. The in vitro infectivity of C. parvum on human ileocecal adenocarcinoma cells (HCT-8) was effectively reduced (> 2 log10) by irradiation at 10 kGy or more. However, in the experiment to find out repair of the infectivity, recovery was not noted until day 7 post-incubation.

Citations

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  • Ultrastructural Changes inCryptosporidium parvumOocysts by Gamma Irradiation
    Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu
    The Korean Journal of Parasitology.2011; 49(1): 25.     CrossRef
  • Effect of Infrared Irradiation of Oocysts on the Pathology of Experimental Cryptosporidiosis in Apparently Normal and Immunosuppressed Mice
    Hafidh I. Al-Sadi, Saevan S. Al-Mahm
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    Jae-Ran Yu
    Hanyang Medical Reviews.2010; 30(3): 187.     CrossRef
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    Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu
    Experimental Parasitology.2010; 125(3): 230.     CrossRef
  • 7,992 View
  • 67 Download
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Antibody Responses to Cryptosporidium Antigen in HIV-positive Patients in the Republic of Korea
Sang-Mee Guk, Jong-Yil Chai, Yung-Oh Shin, Min Seo
Korean J Parasitol 2008;46(2):71-75.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.71

The diagnosis of cryptosporidiosis has been carried out using coprologic techniques in the Republic of Korea. However, antibody responses to Cryptosporidium have rarely been studied. Serum antibodies from HIV-positive/oocyst-positive Korean patients recognized significantly 31 and 27 kDa antigens, and HIV-negative/oocyst-positive individuals clearly reacted to 15/17 kDa antigens. Compared with oocyst-positive cases, 18.7% and 75.8% of sera from HIV-positive patients reacted to 31 and 27 kDa antigens. Only 11.1% of HIV-negative individuals reacted to 15/17 kDa. Based on these findings, serum antibody responses were different between HIV-positive and HIV-negative individuals infected with Cryptosporidium, and it is suggested that HIV-positive patients are more frequently exposed to C. parvum compared to HIV-negative individuals.

Citations

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    Sung-Tae Hong, Tai-Soon Yong
    Infection & Chemotherapy.2020; 52(3): 427.     CrossRef
  • Systemic Antibody Responses to the Immunodominant p23 Antigen and p23 Polymorphisms in Children with Cryptosporidiosis in Bangladesh
    Edward T. Ryan, Elena Naumova, Mohammad M. Karim, Anoli J. Borad, Sitara Swarna Rao Ajjampur, Honorine D. Ward, Gagandeep Kang, Joy Moy, Geneve M. Allison, Stephen B. Calderwood, Sabeena Ahmed, Patricia L. Hibberd, Anne V. Kane, Wasif A. Khan, David Wang
    The American Journal of Tropical Medicine and Hygiene.2012; 86(2): 214.     CrossRef
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Brief Communication

Time gap between oocyst shedding and antibody responses in mice infected with Cryptosporidium parvum
Jae-Ran Yu, Soo-Ung Lee
Korean J Parasitol 2007;45(3):225-228.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.225

We observed the time gap between oocyst shedding and antibody responses in mice (3-week-old C57BL/6J females) infected with Cryptosporidium parvum. Oocyst shedding was verified by modified acid-fast staining. The individually collected mouse sera were assessed for C. parvum IgM and IgG antibodies by enzyme-linked immunosorbent assay from 5 to 25 weeks after infection. The results showed that C. parvum oocysts were shed from day 5 to 51 post-infection (PI). The IgM antibody titers to C. parvum peaked at week 5 PI, whereas the IgG antibody titers achieved maximum levels at week 25 PI. The results revealed that IgM responses to C. parvum infection occurred during the early stage of infection and overlapped with the oocyst shedding period, whereas IgG responses occurred during the late stage and was not correlated with oocyst shedding. Hence, IgM antibody detection may prove helpful for the diagnosis of acute cryptosporidiosis, and IgG antibody detection may prove effective for the detection of past infection and endemicity.

Citations

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  • Anticryptosporidial action mechanisms of Launaea spinosa extracts in Cryptosporidium parvum experimentally infected mice in relation to its UHPLC-MS metabolite profile and biochemometric tools
    Mai M. Elghonemy, Mohamed G. Sharaf El-Din, Dina Aboelsoued, Mohamed F. Abdelhameed, Mohamed A. El-Saied, Nagwa I. Toaleb, Mohamed A. Farag, Abdelsamed I. Elshamy, Abdelbaset M. Elgamal, Horacio Bach
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  • Evaluation of a vaccine candidate isolated from Cryptosporidium parvum oocyst in mice
    Dina Aboelsoued, Hend H. A. M. Abdullah, Kadria N. Abdel Megeed, Soad E. Hassan, Nagwa I. Toaleb
    Veterinary World.2022; : 2772.     CrossRef
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    N. A. Filatova, N. A. Knyazev, S. O. Skarlato, O. V. Anatskaya, A. E. Vinogradov
    Parasite Immunology.2018;[Epub]     CrossRef
  • Development and evaluation of the first immunochromatographic test that can detect specific antibodies against Cryptosporidium parvum
    Ragab M. Fereig, Hanan H. Abdelbaky, Fumiaki Ihara, Yoshifumi Nishikawa
    Acta Tropica.2018; 185: 349.     CrossRef
  • Diagnostic biomarkers in murine Cryptosporidiosis: dose- and age-related infection
    Hebat-Allah S. Yousof, Mona M. Khater, Shaimaa H. El-Sayed, Ayman A. El-Badry
    Journal of Parasitic Diseases.2017; 41(3): 831.     CrossRef
  • Experimental infection with Cryptosporidium parvum IIaA21G1R1 subtype in immunosuppressed mice
    Valeria F. Del Coco, María A. Córdoba, Alicia Sidoti, Mónica Santín, Ricardo Drut, Juan A. Basualdo
    Veterinary Parasitology.2012; 190(3-4): 411.     CrossRef
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Original Article

Role of murine Peyer's patch lymphocytes against primary and challenge infections with Cryptosporidium parvum
Sang-Mee Guk, Jong-Yil Chai
Korean J Parasitol 2007;45(3):175-180.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.175

In order to determine the role of Peyer's patch lymphocytes (PPL) in self-clearing of Cryptosporidium parvum infection in murine models, changes in PPL subsets, their cytokine expression, and in vitro IgG1 and IgA secretions by PPL were observed in primary- and challenge-infected C57BL/6 mice. In primary-infected mice, the percentages of CD4+ T cells, CD8+ T cells, sIgA+ B cells, IL-2+ T cells, and IFN-γ+ T cells among the PPL, increased significantly (P < 0.05) on day 10 post-infection (PI). Secretion of IgG1 and IgA in vitro by PPL also increased on day 10 PI. However, all these responses, with the exception of IgG1 and IgA secretions, decreased in challenge-infected mice on day 7 post-challenge (= day 13 PI); their IgG1 and IgA levels were higher (P > 0.05) than those in primary-infected mice. The results suggest that murine PPL play an important role in self-clearing of primary C. parvum infections through proliferation of CD4+, CD8+, IL-2+, and IFN-γ+ T cells, and IgG1 and IgA-secreting B cells. In challenge infections, the role of T cells is reduced whereas that of B cells secreting IgA appeared to be continuously important.

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  • CRYPTOSPORIDIUM SPECIES AND GIARDIA SPECIES PREVALENCE IN MARINE MAMMAL SPECIES PRESENT IN THE GERMAN NORTH AND BALTIC SEAS
    Miguel L. Grilo, L?dia Gomes, Peter Wohlsein, Lu?s Madeira de Carvalho, Ursula Siebert, Kristina Lehnert
    Journal of Zoo and Wildlife Medicine.2018; 49(4): 1002.     CrossRef
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    Yaning Liu, Shijian Luo, Li Kou, Chaogang Tang, Ruxun Huang, Zhong Pei, Zhendong Li
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  • Dynamics of gut mucosal and systemic Th1/Th2 cytokine responses in interferon-gamma and interleukin-12p40 knock out mice during primary and challenge Cryptosporidium parvum infection
    Tesfaye Sisay Tessema, Bettina Schwamb, Matthias Lochner, Irmgard Förster, Vera Jakobi, Franz Petry
    Immunobiology.2009; 214(6): 454.     CrossRef
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Brief Communications

A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea
Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai
Korean J Parasitol 2006;44(4):367-372.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.367

In order to determine the distribution and prevalence of human cryptosporidiosis on western and southern coastal islands of Jeollanam-do (Province), fecal samples were collected from 2,541 people residing on 25 islands, 13 in the western coasts and 12 in the southern coasts, during July and August 2000. Fecal smears were prepared following formalin-ether sedimentation of the samples and stained by a modified acid-fast procedure. The presence of Cryptosporidium oocysts was determined by light microscopy. Cryptosporidium oocysts were detected in 38 specimens (1.5%). The oocyst positive rate varied (0-6.0%) according to island; the highest was detected on Oenarodo (6.0%), followed by Naenarodo (5.6%) and Nakwoldo (5.4%). The majority (35 persons, 94.6%) of Cryptosporidium-infected individuals were older than 50 years of age. Men (22/1,159; 1.9%) were infected at a higher rate than women (16/1,382; 1.2%). The results of the present survey indicate that human Cryptosporidium infections (due to Cryptosporidium hominis and/or C. parvum) are maintained at a relatively low prevalence on coastal islands of Jeollanam-do, Republic of Korea.

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    Katymilla Guimarães Girotto, Daliane Faria Grama, Maria Júlia Rodrigues da Cunha, Elaine Silva Marques Faria, Jean Ezequiel Limongi, Rogério de Melo Costa Pinto, Márcia Cristina Cury
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    E. Budu‐Amoako, S. J. Greenwood, B. R. Dixon, L. Sweet, L. Ang, H. W. Barkema, J. T. McClure
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    Hyeng-Il CHEUN, Shin-Hyeong CHO, Yi-Young LIM, Byung-Chul LEE, Jung-Yeon KIM, Jung-Won JU, Byoung-Kuk NA, Isao KIMATA, Jae-Ran YU, Tong-Soo KIM
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Intestinal protozoan infections and echinococcosis in the inhabitants of Dornod and Selenge, Mongolia (2003)
Sun Huh, Jae-Ran Yu, Jong-Il Kim, Choijamts Gotov, Radnaabazar Janchiv, Jeong-Sun Seo
Korean J Parasitol 2006;44(2):171-174.
Published online June 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.2.171

The status of intestinal parasitic infections and seroprevalence of cystic echinococcosis in the inhabitants in Dornod and Selenge, Mongolia, was observed with stool and serum samples from 165 and 683 residents from August 9 to August 15, 2003. A total of 10 inhabitants (6.1%) were found to be infected with protozoan cysts or oocysts by stool examinations; 7, 1 and 2 cases with Entamoeba coli, Giardia lamblia and Cryptosporidium parvum, respectively. A total of 62 people (9.1%) revealed significantly high levels of specific antibodies against Echinococcus granulosus by enzyme-linked immunosorbent assay (ELISA). The surveyed areas are rural areas and the sanitized tab water is not available. There is a possibility of endemic transmission of water-borne protozoan diseases in these areas. The clinical echinococcosis is needed to be searched from the seropositive inhabitants.

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Original Article

Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea
Jae-Hwan Park, Sang-Mee Guk, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Jong-Yil Chai
Korean J Parasitol 2006;44(1):27-33.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.27

Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified Ziehl-Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases constituted 7 (41.2%) of 17 examined, and goat cases 3 (42.9%) of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.

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Brief Communication

Prevalence of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea
Jong-Kyu Lee, Hyeon-Je Song, Jae-Ran Yu
Korean J Parasitol 2005;43(3):111-114.
Published online September 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.3.111

The present study investigated the prevalence rate of Cryptosporidium parvum as a cause of diarrhea. We examined 942 stools of unidentified reasons occurring in patients in whom no immunosuppression had been detected. We examined the stools for Cryptosporidium parvum via modified acid-fast staining. The clinical records of all of the positive patients were then analyzed. Nine (1%) of the stools among the 942 diarrheal patients were positive for C. parvum. The positive rate in the males was 1.1% (6/522) and the positive rate of the females was 0.7% (3/420). Age distribution revealed that the highest positive rates were in patients in their sixties, with a positive rate of 2.5% (4/158). In the clinical tests, levels of c-reactive protein, erythrocyte sedimentation rates, and neutrophil proportions were normally increased in the peripheral blood, whereas the lymphocyte proportion exhibited a tendency towards decrease. The pathological findings were compatible with an inflammatory reaction in the host.

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Original Article

Parasitic infections in HIV-infected patients who visited Seoul National University Hospital during the period 1995-2003
Sang-Mee Guk, Min Seo, Yun-Kyu Park, Myoung-Don Oh, Kang-Won Choe, Jae-Lip Kim, Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai
Korean J Parasitol 2005;43(1):1-5.
Published online March 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.1.1

The prevalence of parasitic infections was investigated in human immunodeficiency virus (HIV)-infected patients (n = 105) who visited Seoul National University Hospital, Seoul, Korea, during the period from 1995 to 2003. Fecal samples were collected from 67 patients for intestinal parasite examinations, and sputum or bronchoalveolar lavage samples from 60 patients for examination of Pneumocystis carinii. Both samples were obtained from 22 patients. Thirty-three (31.4%) of the 105 were found to have parasitic infections; Cryptosporidium parvum (10.5%; 7/67), Isospora belli (7.5%; 5/67), Clonorchis sinensis (3.0%; 2/67), Giardia lamblia (1.5%; 1/67), Gymnophalloides seoi (1.5%; 1/67), and Pneumocystis carinii (28.3%; 17/60). The hospital records of the 11 intestinal parasite-infected patients showed that all suffered from diarrhea. This study shows that parasitic infections are important clinical complications in HIV-infected patients in the Republic of Korea.

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Brief Communications

Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea
Sang-Mee Guk, Tai-Soon Yong, Soon-Jung Park, Jae-Hwan Park, Jong-Yil Chai
Korean J Parasitol 2004;42(2):85-89.
Published online June 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.2.85

Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI); but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.

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Infection status of pigs with Cryptosporidium parvum
Jae-Ran Yu, Min Seo
Korean J Parasitol 2004;42(1):45-47.
Published online March 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.1.45

To investigate the infection status of pigs with Cryptosporidium parvum, 589 fecal samples were collected from pigs raised at farm in Chungcheongbuk-do and Chungcheongnam-do. Of the 589 pig fecal samples, 62 (10.5%) were positive for C. parvum. The area showing the highest positive rate was Dangjin-gun, Chungcheongnam-do (14.0%), and the lowest (0%) Salmi-myon, Chungcheongbuk-do. The positive rate of C. parvum in Judok-eup increased from 12.7% in the winter to 22.1% in the summer. The results of this study suggest that the pigs may be a source of human C. parvum infection.

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Original Article

Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea
Jae-Ran Yu, Jong-Kyu Lee, Min Seo, Seok-Il Kim, Woon-Mok Sohn, Sun Huh, Hae-Yeon Choi, Tong-Soo Kim
Korean J Parasitol 2004;42(1):1-6.
Published online March 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.1.1

The present study was undertaken to investigate the infection status of Cryptosporidium parvum in the villagers and the reservoir hosts in several rural areas in Korea. A total 5,262 fecal samples were collected from the inhabitants residing at Gangwon-do, Chungcheongbuk-do, Jeollanam-do, and Gyeongsangnam-do between the dates of September, 2001 to June, 2002. In addition, 1,453 fecal samples were collected from livestock reared in Gokseong-gun, Jeollanam-do and Chungju-si, Chungcheongbuk-do. All the fecal smears were prepared by formalin-ether sedimentation, and examined by light microscopy after modified acid-fast staining. The overall positive rate of human cryptosporidiosis was 3.3%. Gokseong-gun, Jeollanam-do showed a 8.2% positive rate and appeared as the highest endemic area among the surveyed areas. Haman-gun, Gyeongsangnam-do showed a 0.4% positive rate and was the lowest endemic area. The positive rate of livestock infection in Gokseong-gun, Jeollanam-do was 94%, which was more than ten times higher than that of Chungju-si, Chungcheongbuk-do (9.3%). From these results, it was revealed that cryptosporidiosis was an endemic disease in some rural areas of Korea, and the livestock could be an important source of human infection.

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Brief Communication

An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun, Kangwon-do
Min Seo, Sun Huh, Jong-Yil Chai, Jae-Ran Yu
Korean J Parasitol 2001;39(2):201-203.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.201

The present study was undertaken to know the infection status of Cryptosporidium parvum among the residents of Chorwon-gun, Kangwon-do in 1993. Total 461 fecal samples were collected from the inhabitants residing in Chorwon-gun during the period of August 12 to September 14, 1993. Fecal smears were prepared by formalin-ether sedimentation, and examined after modified acid fast staining. Of the 461 fecal samples, 9 (1.9%) were positive for C. parvum oocysts. The positive cases were limited to thirties (4) patients, forties (3), and sixties (2), and no oocyst was detected in other age groups. The oocyst positive rate for male was 1.4% and that of female was 2.6%.

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    Eun-Joo Cho, Jin-Young Yang, Eun-Sook Lee, Se-Chul Kim, So-Yang Cha, Sung-Tek Kim, Man-Ho Lee, Sun-Hee Han, Young-Sang Park
    The Korean Journal of Parasitology.2013; 51(4): 461.     CrossRef
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    Mok-Young Lee, Sang-Jung Park, Eun-Joo Cho, Su-Jeong Park, Sun-Hee Han, Oh-Sang Kwon
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    The Korean Journal of Parasitology.2006; 44(1): 27.     CrossRef
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    Jae-Ran Yu, Jong-Kyu Lee, Min Seo, Seok-Il Kim, Woon-Mok Sohn, Sun Huh, Hae-Yeon Choi, Tong-Soo Kim
    The Korean Journal of Parasitology.2004; 42(1): 1.     CrossRef
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Original Article
The effect of microfilament inhibitor on the Cryptosporidium infection in vitro
Jae-Ran Yu, Sung-Don Choi
Korean J Parasitol 2000;38(4):257-261.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.257

This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.

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