Parasites, Hosts and Diseases

"immunoblot"

Original Articles

Alterations in immunized antigens of Anisakis pegreffii by ampicillin-induced gut microbiome changes in mice: Myungjun Kim, Jun Ho Choi, Myung-hee Yi, Singeun Oh, Tai-Soon Yong, Ju Yeong Kim; Parasites Hosts Dis 2024;62(3):351-364.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.23114

Abstract
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The gut microbiome plays an essential role in host immune responses, including allergic reactions. However, commensal gut microbiota is extremely sensitive to antibiotics and excessive usage can cause microbial dysbiosis. Herein, we investigated how changes in the gut microbiome induced by ampicillin affected the production of IgG1 and IgG2a antibodies in mice subsequently exposed to Anisakis pegreffii antigens. Ampicillin treatment caused a notable change in the gut microbiome as shown by changes in both alpha and beta diversity indexes. In a 1-dimensional immunoblot using Anisakis-specific anti-mouse IgG1, a 56-kDa band corresponding to an unnamed Anisakis protein was detected using mass spectrometry analysis only in ampicillin-treated mice. In the Anisakis-specific anti-mouse IgG2a-probed immunoblot, a 70-kDa band corresponding to heat shock protein 70 (HSP70) was only detected in ampicillin-treated and Anisakis-immunized mice. A 2-dimensional immunoblot against Anisakis extract with immunized mouse sera demonstrated altered spot patterns in both groups. Our results showed that ampicillin treatment altered the gut microbiome composition in mice, changing the immunization response to antigens from A. pegreffii. This research could serve as a basis for developing vaccines or allergy immunotherapies against parasitic infections.

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A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women: Imen Khammari, Fatma Saghrouni, Sami Lakhal, Aida Bouratbine, Moncef Ben Said, Jalel Boukadida; Korean J Parasitol 2014;52(5):493-499.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.493

Abstract
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The determination of the accurate immune status of pregnant women is crucial in order to prevent congenital toxoplasmosis. Equivocal results with conventional serological techniques are not uncommon when IgG titers are close to the cut-off value of the test, so that a confirmatory technique is needed. For this purpose, we developed a homemade immunoblot (IB) using soluble extract of Toxoplasma gondii tachyzoites and assessed it by testing 154 positive, 100 negative, and 123 equivocal sera obtained from pregnant women. In order to select the more valuable bands in terms of sensitivity and specificity, we used the Youden Index (YI). The highest YIs were those given by the 32, 36, 98, 21, and 33 bands. The simultaneous presence on the same blot of at least 3 bands showed a much higher YI (0.964) and was adapted as the positivity criterion. The analysis of results showed that our homemade IB correlated well with the commercial LDBIO Toxo II IgG® kit recently recommended as a confirmatory test (96.7% of concordance).

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Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges
Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barb
Journal of Tropical Medicine.2024; 2024: 1. CrossRef
Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection
Min-ju Kim, Soeun J. Park, Hyunwoo Park
European Journal of Medical Research.2024;[Epub] CrossRef
Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
Valeria Meroni, Francesca Genco, Luigia Scudeller, Marie-Pierre Brenier-Pinchart, Hélène Fricker-Hidalgo, Coralie L’Ollivier, Luc Paris, Hervé Pelloux
Pathogens.2022; 11(6): 665. CrossRef
Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens
Vincent Jean-Pierre, Julien Miozzo, Hélène Fricker-Hidalgo, Cécile Garnaud, Marie Gladys Robert, Hervé Pelloux, Marie-Pierre Brenier-Pinchart
Parasite.2022; 29: 52. CrossRef
A longitudinal study of Toxoplasma gondii seroconversion on four large Danish sow farms
Abbey Olsen, Lis Alban, Matthew Denwood, Hans Houe, Tina Birk Jensen, Henrik Vedel Nielsen
Veterinary Parasitology.2021; 295: 109460. CrossRef
Performance of seven commercial automated assays for the detection of low levels of anti-Toxoplasma IgG in French immunocompromised patients
Tiphaine Douet, Catherine Armengol, Elena Charpentier, Pamela Chauvin, Sophie Cassaing, Xavier Iriart, Antoine Berry, Judith Fillaux
Parasite.2019; 26: 51. CrossRef
Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays
Bettie Voordouw, Barry Rockx, Thomas Jaenisch, Pieter Fraaij, Philippe Mayaud, Ann Vossen, Marion Koopmans
Clinical Microbiology Reviews.2019;[Epub] CrossRef
Semiquantitative Dot Blot with the GRA8 antigen to differentiate the stages of toxoplasmosis infection
Juan Gabriel Costa, María Julia Vilariño
Journal of Microbiological Methods.2018; 149: 9. CrossRef
Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico
Cosme Alvarado-Esquivel, Luis Sánchez-Anguiano, Luis Berumen-Segovia, Jesús Hernández-Tinoco, Yazmin Rico-Almochantaf, Alfredo Cisneros-Camacho, Jorge Cisneros-Martínez
International Journal of Environmental Research and Public Health.2017; 14(11): 1413. CrossRef
Sensing parasites: Proteomic and advanced bio-detection alternatives
Carlos Sánchez-Ovejero, Fernando Benito-Lopez, Paula Díez, Adriano Casulli, Mar Siles-Lucas, Manuel Fuentes, Raúl Manzano-Román
Journal of Proteomics.2016; 136: 145. CrossRef
The impact of lowering the cut-off value on the sensitivity of the Platelia Elisa IgG (Bio-Rad) test for toxoplasmosis diagnosis
Oussama Mouri, Eric Kendjo, Feriel Touafek, Arnaud Fekkar, Ousmane Konte, Sebastien Imbert, Régis Courtin, Dominique Mazier, Luc Paris
Parasite.2015; 22: 22. CrossRef

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Brief Communications

Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes: Eun-Taek Han, Hyun-Jong Yang, Young-Jin Park, Jeong-Hyun Park, Jong-Yil Chai; Korean J Parasitol 2014;52(2):201-204.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.201

Abstract
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This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

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Reliability of heterophyid antigens in heterologous protection against human schistosomiasis
Alaa H. A. Hegazy, Lamia A. Galal, Tasneem M. Hassan, Refaat M. A. Khalifa
Journal of Parasitic Diseases.2020; 44(2): 349. CrossRef
Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef

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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand: Amnat Kitkhuandee, Waranon Munkong, Kittisak Sawanyawisuth, Penchom Janwan, Wanchai Maleewong, Pewpan M. Intapan; Korean J Parasitol 2013;51(6):755-757.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.755

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Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the frequency of G. spinigerum infection in non-traumatic SAH. A series of patients diagnosed as non-traumatic SAH at the Srinagarind Hospital, Khon Kaen University, Thailand between January 2011 and January 2013 were studied. CT or MR imaging of the brain was used for diagnosis of SAH. Patients were categorized as aneurysmal subarachnoid hemorrhage (A-SAH) or non-aneurysmal subarachnoid hemorrhage (NA-SAH) according to the results of cerebral angiograms. The presence of specific antibodies in serum against 21- or 24-kDa G. spinigerum antigen was determined using the immunoblot technique. The detection rate of antibodies was compared between the 2 groups. Of the 118 non-traumatic SAH patients for whom cerebral angiogram and immunoblot data were available, 80 (67.8%) patients had A-SAH, whereas 38 (32.2%) had NA-SAH. Overall, 23.7% were positive for specific antibodies against 21- and/or 24-kDa G. spinigerum antigen. No significant differences were found in the positive rate of specific antibodies against G. spinigerum in both groups (P-value=0.350).

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A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis: Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong; Korean J Parasitol 2013;51(6):751-754.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.751

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Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.

Citations

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Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information
Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong
Scientific Reports.2022;[Epub] CrossRef
Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis
Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
Diagnostics.2021; 11(5): 862. CrossRef
Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong
Pathogens.2021; 10(9): 1080. CrossRef
Combining lexical and context features for automatic ontology extension
Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf
Journal of Biomedical Semantics.2020;[Epub] CrossRef
Human gnathostomiasis: a neglected food-borne zoonosis
Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao
Parasites & Vectors.2020;[Epub] CrossRef
Surveillance and diagnosis of zoonotic foodborne parasites
Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
Food Science & Nutrition.2018; 6(1): 3. CrossRef

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IgG Western Blot for Confirmatory Diagnosis of Equivocal Cases of Toxoplasmosis by EIA-IgG and Fluorescent Antibody Test: Imen Khammari, Fatma Saghrouni, Alia Yaacoub, Sondoss Gaied Meksi, Hinda Ach, Lamia Garma, Akila Fathallah, Moncef Ben Sa?d; Korean J Parasitol 2013;51(4):485-488.
Published online August 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.4.485

Abstract
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The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo II IgG Western blot (IB) showed an excellent correlation with the dye test. We estimated the proportion of equivocal and discordant results between the enzyme immunoassay Platelia Toxo IgG (EIA-IgG) and fluorescent antibody test (FAT) and assessed the usefulness of the IB as a confirmatory test. Out of 2,136 sera collected from pregnant women, 1,644 (77.0%) tested unequivocally positive and 407 (19.0%) were negative in both EIA-IgG and FAT. The remaining 85 (4%) sera showed equivocal or discordant results. Among them, 73 (85.9%) were positive and 12 (14.1%) were negative in IB. Forty-one (89.1%) equivocal sera in EIA-IgG and 46 (86.8%) equivocal sera in FAT were positive in IB. Reducing the cut-off values of both screening techniques improved significantly their sensitivity in detecting very low IgG titers at the expense of their specificity. In conclusion, equivocal results in routine-used techniques and their discordance in determination of the immune status in pregnancy women were not uncommon. IB test appeard to be highly useful in these situations as a confirmatory technique.

Citations

Citations to this article as recorded by

Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges
Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barb
Journal of Tropical Medicine.2024; 2024: 1. CrossRef
Congenital Toxoplasmosis Diagnosis: Current Approaches and New Insights
Alfredo Márquez-Mauricio, Heriberto Caballero-Ortega, Fernando Gómez-Chávez
Acta Parasitologica.2023; 68(3): 473. CrossRef
Performances of ICT Toxoplasma IgG-IgM test in comparison with Vidas® toxo competition to determine the immune status against Toxoplasma gondii
Sylvie Abraham, Raphael Piarroux, Ying Zhou, Vera Tesic, Ana Abeleda, Nadhira Houhou-Fidouh, Pascale Nicaise-Rolland, Luce Landraud, Rima McLeod, Sandrine Houzé
European Journal of Clinical Microbiology & Infectious Diseases.2023; 42(11): 1327. CrossRef
Determination of an optimal ELISA cut-off for the diagnosis of Toxoplasma gondii infection in pigs using Bayesian latent class modelling of data from multiple diagnostic tests
Abbey Olsen, Henrik Vedel Nielsen, Lis Alban, Hans Houe, Tina Birk Jensen, Matthew Denwood
Preventive Veterinary Medicine.2022; 201: 105606. CrossRef
Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
Valeria Meroni, Francesca Genco, Luigia Scudeller, Marie-Pierre Brenier-Pinchart, Hélène Fricker-Hidalgo, Coralie L’Ollivier, Luc Paris, Hervé Pelloux
Pathogens.2022; 11(6): 665. CrossRef
Difficultés d’interprétation de la sérologie toxoplasmose
Luc Paris, Sandrine Houzé
Revue Francophone des Laboratoires.2022; 2022(545): 33. CrossRef
Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens
Vincent Jean-Pierre, Julien Miozzo, Hélène Fricker-Hidalgo, Cécile Garnaud, Marie Gladys Robert, Hervé Pelloux, Marie-Pierre Brenier-Pinchart
Parasite.2022; 29: 52. CrossRef
Contribution of the Toxoplasma ICT IgG IgM® test in determining the immune status of pregnant women against toxoplasmosis
Ben‐Abdallah Rym, Kalboussi Yasmine, Bellali Hedia, Issaoui Nesrine, Souissi Olfa, Maatoug Rania, Aoun Karim, Bouratbine Aïda
Journal of Clinical Laboratory Analysis.2021;[Epub] CrossRef
A longitudinal study of Toxoplasma gondii seroconversion on four large Danish sow farms
Abbey Olsen, Lis Alban, Matthew Denwood, Hans Houe, Tina Birk Jensen, Henrik Vedel Nielsen
Veterinary Parasitology.2021; 295: 109460. CrossRef
Contribution of the "Recomline Toxoplasma IgM" Kit in the Distinction between Toxoplasmic IgM and Natural IgM
Khammari Imen, Ben Halima Nada, Ismaïl Samar, Chouaieb Hamed, Fathallah Akila
International Journal Of Pharmaceutical And Phytopharmacological Research.2021; 11(6): 15. CrossRef
Evaluating the “Recomline Toxoplasma IgM” Kit for Differentiating Toxoplasmic and Natural IgM
Imen Khammari, Nada Ben Halima, Samar Ismaïl, Hamed Chouaieb, Akila Fathallah
Interdisciplinary Research in Medical Sciences Specialty.2021; 1(2): 1. CrossRef
Performance of a Toxo IgM prototype assay for the diagnosis of maternal and congenital Toxoplasma infections
Martine Wallon, Hélène Fricker-Hidalgo, Emmanuelle Chapey, Claire Bailet, Céline Dard, Marie-Pierre Brenier-Pinchart, Hervé Pelloux
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(11): 1959. CrossRef
Performance of Zika Assays in the Context of Toxoplasma gondii, Parvovirus B19, Rubella Virus, and Cytomegalovirus (TORCH) Diagnostic Assays
Bettie Voordouw, Barry Rockx, Thomas Jaenisch, Pieter Fraaij, Philippe Mayaud, Ann Vossen, Marion Koopmans
Clinical Microbiology Reviews.2019;[Epub] CrossRef
Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening
Aline de Castro Zacche-Tonini, Giuliana Schmidt França Fonseca, Laura Néspoli Nassar Pansini de Jesus, Geisa Baptista Barros, Jordana Grazziela Alves Coelho-dos-Reis, Samantha Ribeiro Béla, Anderson Silva Machado, Ana Carolina Aguiar Vasconcelos Carneiro,
Journal of Immunological Methods.2017; 451: 37. CrossRef
A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Imen Khammari, Fatma Saghrouni, Sami Lakhal, Aida Bouratbine, Moncef Ben Said, Jalel Boukadida
The Korean Journal of Parasitology.2014; 52(5): 493. CrossRef
Identification of soluble and membrane antigenic markers of acquired toxoplasmosis by immunoblot
I. Khammari, F. Saghrouni, S. Lakhal, I. Bougmiza, A. Bouratbine, M. Ben Said, J. Boukadida
Parasite Immunology.2014; 36(12): 684. CrossRef

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Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity: Hyun-Jong Yang; Korean J Parasitol 2012;50(2):181-183.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.181

Abstract
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The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

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Immunoblot Patterns of Taenia asiatica Taeniasis: Hyeong-Kyu Jeon, Keeseon S. Eom; Korean J Parasitol 2009;47(1):73-77.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.73

Abstract
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Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight proteins reactive to serum IgG were observed between 121 and 10 kDa in adult worms, and more than 22 serum-reactive components between 97 kDa and 21.5 kDa were observed in eggs of T. asiatica. Antigens of adult T. asiatica revealed immunoblot bands between 120 and 21.5 kDa against T. asiatica infected sera. Antigens of adult Taenia saginata revealed 110-100, 66, 58-56, and 46 kDa immunoblot bands against T. asiatica infected sera. Antigens of adult Taenia solium also revealed 99-97, 68-66, and 46 kDa bands against T. asiatica infected sera. The immunoblot band of 21.5 kDa exhibited specificity to T. asiatica.

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Evaluation of enzyme-linked immunoelectrotransfer blot for diagnosis of cysticercosis in swine from North India
K.K. Gupta, A. Singh, A.K. Singh, S.K. Singh, M. Tripathi, R.K. Gupta, K.N. Prasad
Journal of Helminthology.2019; 93(05): 548. CrossRef
Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt
Nasr M. El-Bahy, Eman K. Bazh, Shimaa S. Sorour, Nagwa M. Elhawary
Parasitology Research.2017; 116(4): 1129. CrossRef
Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe
M. A. Gómez-Morales, T. Gárate, J. Blocher, B. Devleesschauwer, G. S. A. Smit, V. Schmidt, M. J. Perteguer, A. Ludovisi, E. Pozio, P. Dorny, S. Gabriël, A. S. Winkler
European Journal of Clinical Microbiology & Infectious Diseases.2017; 36(11): 2029. CrossRef
Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools
Dinh Ng-Nguyen, Mark A. Stevenson, Pierre Dorny, Sarah Gabriël, Tinh Van Vo, Van-Anh Thi Nguyen, Trong Van Phan, Sze Fui Hii, Rebecca J. Traub, Alessandra Morassutti
PLOS Neglected Tropical Diseases.2017; 11(7): e0005743. CrossRef
Epidemiology and genetic diversity of Taenia asiatica: a systematic review
Anita Ale, Bjorn Victor, Nicolas Praet, Sarah Gabriël, Niko Speybroeck, Pierre Dorny, Brecht Devleesschauwer
Parasites & Vectors.2014;[Epub] CrossRef
An immunoblot for detection of Taenia saginata cysticercosis
Sameh Abuseir, Uschi Nagel-Kohl, Sonja Wolken, Christina Strube
Parasitology Research.2013; 112(5): 2069. CrossRef
Molecular Approaches to Taenia asiatica
Hyeong-Kyu Jeon, Keeseon S. Eom
The Korean Journal of Parasitology.2013; 51(1): 1. CrossRef
Taenia asiatica: the Most Neglected Human Taenia and the Possibility of Cysticercosis
M. Teresa Galán-Puchades, Mario V. Fuentes
The Korean Journal of Parasitology.2013; 51(1): 51. CrossRef
Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
The Korean Journal of Parasitology.2013; 51(6): 719. CrossRef
Control of cysticercosis in Madagascar: beware of the pitfalls
Harentsoaniaina Rasamoelina-Andriamanivo, Vincent Porphyre, Ronan Jambou
Trends in Parasitology.2013; 29(11): 538. CrossRef
Molecular identification of Taenia specimens after long-term preservation in formalin
Hyeong-Kyu Jeon, Kyu-Heon Kim, Keeseon S. Eom
Parasitology International.2011; 60(2): 203. CrossRef

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Original Articles

Seroprevalence of Toxocariasis among Healthy People with Eosinophilia: Yong-Hun Kim, Sun Huh, Young-Bae Chung; Korean J Parasitol 2008;46(1):29-32.
Published online March 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.1.29

Abstract
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The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.

Citations

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Keys to Unlock the Enigma of Ocular Toxocariasis: A Systematic Review and Meta-analysis
Milad Badri, Aida Vafae Eslahi, Meysam Olfatifar, Sahar Dalvand, Elham Houshmand, Amir Abdoli, Hamidreza Majidiani, Ali Eslami, Mohammad Zibaei, Morteza Ghanbari Johkool, Ali Taghipour, Sima Hashemipour
Ocular Immunology and Inflammation.2021; 29(7-8): 1265. CrossRef
Urticaria and silent parasitism by Ascaridoidea: Component-resolved diagnosis reinforces the significance of this association
Marta Viñas, Idoia Postigo, Ester Suñén, Jorge Martínez, Maria Victoria Periago
PLOS Neglected Tropical Diseases.2020; 14(4): e0008177. CrossRef
A Systematic Review and Meta-analysis of Toxocariasis in Iran: Is it Time to Take it Seriously?
Mohammad Javad Abbaszadeh Afshar, Farzaneh Zahabiun, Peyman Heydarian, Hossein Mozafar Saadati, Sina Mohtasebi, Farzad Khodamoradi, Vahid Raissi
Acta Parasitologica.2020; 65(3): 569. CrossRef
Prevalence of Toxocariasis and Its Risk Factors in Patients with Eosinophilia in Korea
Hyun Beom Song, Deokho Lee, Yan Jin, Jinwoo Kang, Shin-Hyeong Cho, Min Sun Park, Jin-Ho Park, Woo-Jung Song, Hye-Ryun Kang, Sang Hyub Lee, Sung-Tae Hong, Min-Ho Choi
The Korean Journal of Parasitology.2020; 58(4): 413. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
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Organ-specific antigens of Clonorchis sinensis: Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2004;42(4):169-174.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.169

Abstract
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This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea: Jong-Yil Chai, Aifen Lin, Eun-Hee Shin, Myoung-Don Oh, Eun-Taek Han, Ho-Woo Nam, Soon-Hyung Lee; Korean J Parasitol 2003;41(3):147-154.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.147

Abstract
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Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.

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Brief Communication

Cross-reactivity between sera from dogs experimentally infected with Dirofilaria immitis and crude extract of Toxocara canis: Kun-Ho Song, Mineo Hayasaki, Kyu-Woan Cho, Sang-Eun Lee, Duck-Hwan Kim; Korean J Parasitol 2002;40(4):195-198.
Published online December 30, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.4.195

Abstract
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This study was performed to investigate whethere there is cross-reactivity between Dirofilaria immitis and three intestinal nematodes of dogs. In ELISA, D. immitis-infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of T. canis. In immunoblotting, some antigenic fractions, 44, 57, 88, 100 kDa of crude extract of T. canis, were found to be positive reaction with sera of dogs infected with D. immitis. However, little or no cross-reaction were observed between sera of D. immitis-infected dogs and crude extract antigen of T. vulpis or A. caninum. These result suggest that there are partial cross reaction between sera of D. immitis-infected dogs and the antigen of T. canis.

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Original Articles

IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis: Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho; Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Abstract
PDF

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

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Variation of antigenicity and serological reaction to Pneumocystis carinii in Korea: Hyun-Young Park, Soo-Ung Lee, Seoung-Wan Chae, Sun Huh, Jae-Ran Yu, Jin Kim, Sung-Tae Hong; Korean J Parasitol 1999;37(2):109-115.
Published online June 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.2.109

Abstract
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The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon, Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-Linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.

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Human Immunodeficiency Virus-Infected Patients with PriorPneumocystisPneumonia Exhibit Increased Serologic Reactivity to Several Major Surface Glycoprotein Clones
K. R. Daly, J. V. Koch, N. J. Shire, L. Levin, P. D. Walzer
Clinical and Vaccine Immunology.2006; 13(10): 1071. CrossRef