Parasites, Hosts and Diseases

"species"

Original Articles

CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products: Young Ah Lee, Myeong Heon Shin; Parasites Hosts Dis 2024;62(3):270-280.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24046

Abstract
PDF

Trichomoniasis is caused by a sexually transmitted flagellate protozoan parasite Trichomonas vaginalis. T. vaginalis-derived secretory products (TvSP) contain lipid mediators such as leukotriene B₄ (LTB₄) and various cysteinyl leukotrienes (CysLTs) which included LTC₄, LTD₄, and LTE₄. However, the signaling mechanisms by which T. vaginalis-induced CysLTs stimulate interleukin (IL)-8 production in human mast cells remain unclear. In this study, we investigated these mechanisms in human mast cells (HMC-1). Stimulation with TvSP resulted in increased intracellular reactive oxygen species (ROS) generation and NADPH oxidase 2 (NOX2) activation compared to unstimulated cells. Pre-treatment with NOX2 inhibitors such as diphenyleneiodonium chloride (DPI) or apocynin significantly reduced ROS production in TvSP-stimulated HMC-1 cells. Additionally, TvSP stimulation increased NOX2 protein expression and the translocation of p47^phox from the cytosol to the membrane. Pretreatment of HMC-1 cells with PI3K or PKC inhibitors reduced TvSP-induced p47^phox translocation and ROS generation. Furthermore, NOX2 inhibitors or NOX2 siRNA prevented CREB phosphorylation and IL-8 gene expression or protein secretion induced by TvSP. Pretreatment with a CysLTR antagonist significantly inhibited TvSP-induced ROS production, CREB phosphorylation, and IL-8 production. These results indicate that CysLT-mediated activation of NOX2 plays a crucial role in ROS-dependent IL-8 production in human mast cells stimulated by T. vaginalis-secreted CysLTs. These findings enhance our understanding of the inflammatory response in trichomoniasis and may inform the development of targeted therapies to mitigate this response.

Citations

Citations to this article as recorded by

Untargeted metabolomics reveals postoperative metabolic dynamics in hepatic cystic echinococcosis patients
Kahaer Tuerxun, Abudouxikuer Abudoumijiti, Zainuer Yusupu, Rousitaimujiang Yimamu, Ronghua Tang, Ziru Wang, Abudoukeyimu Yasheng, Irshat Ibrahim, Yuanquan Wu
Immunobiology.2025; 230(4): 153099. CrossRef
Radical-induced lipid oxidation produces a torrent of leukotriene-like agonists in severe asthma
Si-Yang Liu, Mikhail Linetsky, Abby Hite, Yu-Shiuan Cheng, Masaru Miyagi, Serena C. Zhu, Hong Zeng, Siqi Huang, Myra Qin, Emma Sintic, Carolyn M. Koutures, Abigail Meneses, Olivia R. Laniak, Sailaja Paruchuri, Lakshminarayan R. Teegala, Kaixi Cui, Fariba
Journal of Allergy and Clinical Immunology.2025;[Epub] CrossRef

2,519 View
75 Download
1 Web of Science
Crossref

Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica: Young Ah Lee, Myeong Heon Shin; Parasites Hosts Dis 2023;61(4):388-396.
Published online November 28, 2023; DOI: https://doi.org/10.3347/PHD.23094

Abstract
PDF

Entamoeba histolytica is an enteric tissue-invasive protozoan parasite causing amoebic colitis and liver abscesses in humans. Amoebic contact with host cells activates intracellular signaling pathways that lead to host cell death via generation of caspase-3, calpain, Ca²⁺ elevation, and reactive oxygen species (ROS). We previously reported that various NADPH oxidases (NOXs) are responsible for ROS-dependent death of various host cells induced by amoeba. In the present study, we investigated the specific NOX isoform involved in ROS-dependent death of hepatocytes induced by amoebas. Co-incubation of hepatoma HepG2 cells with live amoebic trophozoites resulted in remarkably increased DNA fragmentation compared to cells incubated with medium alone. HepG2 cells that adhered to amoebic trophozoites showed strong dichlorodihydrofluorescein diacetate (DCF-DA) fluorescence, suggesting intracellular ROS accumulation within host cells stimulated by amoebic trophozoites. Pretreatment of HepG2 cells with the general NOX inhibitor DPI or NOX2-specific inhibitor GSK 2795039 reduced Entamoeba-induced ROS generation. Similarly, Entamoeba-induced LDH release from HepG2 cells was effectively inhibited by pretreatment with DPI or GSK 2795039. In NOX2-silenced HepG2 cells, Entamoeba-induced LDH release was also significantly inhibited compared with controls. Taken together, the results support an important role of NOX2-derived ROS in hepatocyte death induced by E. histolytica.

Citations

Citations to this article as recorded by

CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 270. CrossRef
Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites
Dongjun Kang, Jun Ho Choi, Myungjun Kim, Sohyeon Yun, Singeun Oh, Myung-hee Yi, Tai-Soon Yong, Young Ah Lee, Myeong Heon Shin, Ju Yeong Kim
Scientific Reports.2024;[Epub] CrossRef

3,687 View
158 Download
2 Web of Science
Crossref

Case Report

Two Cases of Mange Mite (Sarcoptes scabiei) Infestation in Long-Tailed Goral (Naemorhedus caudatus) in Republic of Korea: Da Som Park, Jin Choi, Hee-Jong Kim, Jin-Yong Kim, Min-Han Kim, Jin-Young Lee, Jeong Chan Moon, Hee-Bok Park, KyungMin Park, Jun Hee Yun, Yeonsu Oh, Seongjun Choe, Ki-Jeong Na, Jongmin Yoon; Korean J Parasitol 2022;60(6):423-427.
Published online December 22, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.6.423

Abstract
PDF

The long-tailed goral, Naemorhedus caudatus (Mammalia: Bovidae), is one of the endangered animals in the Republic of Korea (Korea). Sarcoptic mange mites infested in diverse species of mammals, including humans, but no case has been reported in long-tailed gorals. We report 2 cases of mange mite, Sarcoptes scabiei, infestation in long-tailed gorals. Mange mites were sampled in the skin legions of the 2 long-tailed gorals, which were rescued in 2 different regions, Uljin-gun, Gyeongsangbuk-do and Cheorwon-gun, Gangwon-do, Korea. Our results showed that the ectoparasite was the itch mite that burrowed into skin and caused scabies on the morphological inspection and placed within the phylogenetic relations of the species. The present study confirmed for the first time in Korea that mange mites are pathogenic scabies of long-tailed goral. Closer surveillance of this pathogenic ectoparasite in zoonotic and infectious ecosystems is warranted.

Citations

Citations to this article as recorded by

Sarcoptic Mange in Reintroduced Red Foxes (Vulpes vulpes) in South Korea: Case Histories, Clinical Assessments, Treatments, and Pathological Findings
Sook-Jin Lee, An-Na Lee, Eun-Bin Shin, Min-Sung Kim, Hyoung-Jin Kim, Doo-Hyun Han, Yong-Sik Jo, Jin-Suk Ahn, Seung-Hoon Chea, Chang-Min Jeong, Hee-Yeon Lee, Seong-Geun Bae, Jeong-Jin Yang
Animals.2025; 15(10): 1491. CrossRef
Genetic and Serological Survey of Sarcoptic Mange (Sarcoptes scabiei) in Wild Boars (Sus scrofa) in South Korea
Sanghyun Lee, Garam Kim, So-Jeong Kim, Weon-Hwa Jheong, Dong-Hyuk Jeong
Animals.2024; 14(23): 3490. CrossRef

7,903 View
140 Download
2 Web of Science
Crossref

Brief Communications

Specific Detection of Acanthamoeba species using Polyclonal Peptide Antibody Targeting the Periplasmic Binding Protein of A. castellanii: Min-Jeong Kim, Fu-Shi Quan, Hyun-Hee Kong, Jong-Hyun Kim, Eun-Kyung Moon; Korean J Parasitol 2022;60(2):143-147.
Published online April 20, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.2.143

Abstract
PDF

Acanthamoeba keratitis (AK) is a rare ocular disease, but it is a painful and sight-threatening infectious disease. Early diagnosis and adequate treatment are necessary to prevent serious complications. While AK is frequently diagnosis via several PCR assays or Acanthamoeba-specific antibodies, a more specific and effective diagnostic method is required. This study described the production of a polyclonal peptide antibody against the periplasmic binding protein (PBP) of A. castellanii and investigated its diagnostic potential. Western blot analysis showed that the PBP antibody specifically reacted with the cell lysates of A. castellanii. However, the PBP antibody did not interact with human corneal epithelial (HCE) cells and the other 3 major causative agents of keratitis. Immunocytochemistry (ICC) results revealed the specific detection of A. castellanii trophozoites and cysts by PBP antibodies when A. castellanii were co-cultured with HCE cells. PBP antibody specificity was further confirmed by co-culture of A. castellanii trophozoites with F. solani, S. aureus, and P. aeruginosa via ICC. The PBP antibody specifically reacted with the trophozoites and cysts of A. polyphaga, A. hatchetti, A. culbertsoni, A. royreba, and A. healyi, thus demonstrated its genus-specific nature. These results showed that the PBP polyclonal peptide antibody of A. castellanii could specifically detect several species of Acanthamoeba, contributing to the development of an effective antibody-based AK diagnostics.

Citations

Citations to this article as recorded by

Evaluation of the potential for diagnosis of fungal keratitis using a Fusarium-specific antibody
Hye-Jeong Jo, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Scientific Reports.2025;[Epub] CrossRef
Detection of Fusarium solani using cutinase antibody and its application in diagnosing fungal keratitis in an animal model
Hye-Jeong Jo, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon, Olaf Kniemeyer
PLOS One.2025; 20(8): e0330455. CrossRef
A chorismate mutase-targeted, core-shell nanoassembly-activated SERS immunoassay platform for rapid non-invasive detection of Acanthamoeba infection
Hyerin Lee, Min-Jeong Kim, Junkyu Chung, Wansun Kim, Hye-Jeong Jo, Tae Gi Kim, Jae-Ho Shin, Gi-Ja Lee, Fu-Shi Quan, Hyun-Hee Kong, Sang Woong Moon, Eun-Kyung Moon, Samjin Choi
Nano Today.2024; 59: 102506. CrossRef
Evaluating the Diagnostic Potential of Chorismate Mutase Poly-Clonal Peptide Antibody for the Acanthamoeba Keratitis in an Animal Model
Min-Jeong Kim, Hye-Jeong Jo, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Pathogens.2023; 12(4): 526. CrossRef
Detection of Acanthamoeba from Acanthamoeba Keratitis Mouse Model Using Acanthamoeba-Specific Antibodies
Min-Jeong Kim, A-Jeong Ham, A-Young Park, Hae-Jin Sohn, Ho-Joon Shin, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon
Microorganisms.2022; 10(9): 1711. CrossRef

3,818 View
196 Download
5 Web of Science
Crossref

Anticoccidial Activity of Berberine against Eimeria-Infected Chickens: Binh Thanh Nguyen, Rochelle Alipio Flores, Paula Leona Taymen Cammayo, Suk Kim, Woo Hyun Kim, Wongi Min; Korean J Parasitol 2021;59(4):403-408.
Published online August 18, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.4.403

Avian coccidiosis has a major economic impact on the poultry industry, it is caused by 7 species of Eimeria, and has been primarily controlled using chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. We assessed anticoccidial effects of berberine-based diets in broiler chickens following oral infection with 5 Eimeria species (E. acervulina, E. maxima, E. tenella, E. mitis, and E. praecox). When 0.2% berberine, a concentration that does not affect weight gain, was added to the diet, the 4 groups infected with E. acervulina, E. tenella, E. mitis, or E. praecox showed significant reductions in fecal oocyst shedding (P<0.05) compared to their respective infected and untreated controls. In chickens treated 0.5% berberine instead of 0.2% and infected with E. maxima, fecal oocyst production was significantly reduced, but body weight deceased, indicating that berberine treatment was not useful for E. maxima infection. Taken together, these results illustrate the applicability of berberine for prophylactic use to control most Eimeria infections except E. maxima. Further studies on the mechanisms underlying the differences in anticoccidial susceptibility to berberine, particularly E. maxima, are remained.

Citations

Citations to this article as recorded by

Evaluation of the Effectiveness of a Phytogenic Supplement (Alkaloids and Flavonoids) in the Control of Eimeria spp. in Experimentally Challenged Broiler Chickens
Anne-Sophie Hascoët, Paulina Torres-Celpa, Roberto Riquelme-Neira, Héctor Hidalgo-Olate
Animals.2025; 15(6): 847. CrossRef
Improving The Sporulation of Eimeria tenella Oocysts Purified From Chicken Faeces
Nurfazila Latif, Aravind Marcus, Paul Bura Thlama, Suhaili Mustafa, Muhamad Hakim Mohd Ali Hanafiah, Lirong Yu Abit, Sharifah Salmah Syed Hussain, Nor Azlina Abdul Aziz, Shu-San Loo, Kiew Lian Wan, Sarah A Altwaim, Juriah Kamaludeen
Malaysian Applied Biology.2025; 54(1): 38. CrossRef
Berberine Reveals Anticoccidial Activity by Influencing Immune Responses in Eimeria acervulina-Infected Chickens
Binh T. Nguyen, Bujinlkham Altanzul, Rochelle A. Flores, Honghee Chang, Woo H. Kim, Suk Kim, Wongi Min
Biomolecules.2025; 15(7): 985. CrossRef
Vaccinations and Functional Feed Supplements as Alternatives to Coccidiostats for the Control of Coccidiosis in Raising Broiler Chickens
Maciej Rosłoń, Edward Majewski, Monika Gębska, Anna Grontkowska, Michał Motrenko, Artur Żbikowski, Monika Michalczuk, Jakub Urban
Animals.2025; 15(17): 2548. CrossRef
Management and control of coccidiosis in poultry — A review
Rafiq Ahmad, Yu-Hsiang Yu, Kuo-Feng Hua, Wei-Jung Chen, Daniel Zaborski, Andrzej Dybus, Felix Shih-Hsiang Hsiao, Yeong-Hsiang Cheng
Animal Bioscience.2024; 37(1): 1. CrossRef
Large-Scale Field Trials of an Eimeria Vaccine Induce Positive Effects on the Production Index of Broilers
Binh T. Nguyen, Dongjean Yim, Rochelle A. Flores, Seung Yun Lee, Woo H. Kim, Seung-Hwan Jung, Sangkyu Kim, Wongi Min
Vaccines.2024; 12(7): 800. CrossRef
An insight into the potential of berberine in animal nutrition: Current knowledge and future perspectives
Navid Ghavipanje, Mohammad Hasan Fathi Nasri, Einar Vargas‐Bello‐Pérez
Journal of Animal Physiology and Animal Nutrition.2023; 107(3): 808. CrossRef
Botanicals: A promising approach for controlling cecal coccidiosis in poultry
Zohaib Saeed, Khalid A. Alkheraije
Frontiers in Veterinary Science.2023;[Epub] CrossRef
Pathological Role of Oxidative Stress in Aflatoxin-Induced Toxicity in Different Experimental Models and Protective Effect of Phytochemicals: A Review
Martha Cebile Jobe, Doctor M. N. Mthiyane, Phiwayinkosi V. Dludla, Sithandiwe E. Mazibuko-Mbeje, Damian C. Onwudiwe, Mulunda Mwanza
Molecules.2023; 28(14): 5369. CrossRef
The Immunoprotective Effect of ROP27 Protein of Eimeria tenella
Menggang Li, Xiaoling Lv, Mingxue Zheng, Yingyi Wei
Animals.2023; 13(22): 3500. CrossRef
Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway
Xuelian Yang, Xinghui Pan, Zhipeng Jia, Bingrong Bai, Wenjing Zhi, Hang Chen, Chunli Ma, Dexing Ma
Veterinary Research.2022;[Epub] CrossRef
Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms
Rochelle A. Flores, Binh T. Nguyen, Paula Leona T. Cammayo, Tuấn Cường Võ, Haung Naw, Suk Kim, Woo H. Kim, Byoung-Kuk Na, Wongi Min
BMC Veterinary Research.2022;[Epub] CrossRef

6,547 View
116 Download
13 Web of Science
Crossref

Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea: Eun Jeong Won, Ju Hyeon Shin, Yu Jeong Lee, Moon-Ju Kim, Seung Ji Kang, Sook In Jung, Soo Hyun Kim, Jong Hee Shin, Jong-Yil Chai, Sung-Shik Shin; Korean J Parasitol 2019;57(3):313-318.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.313

Abstract
PDF

In recent years, the taeniasis has been rarely reported in the Republic of Korea (Korea). But in this study, we intend to report 4 taeniasis cases caused by Taenia saginata during a 5-month period (February to June 2018) at a unversity hospital in Gwangju, Korea. Worm samples (proglottids) discharged from all cases were identified by phenotypic and molecular diagnostics. Mitochondrial cytochrome c oxidase subunit I sequences showed 99.4-99.9% identity with T. saginata but, differed by 4% from T. asiatica and by 7% from T. multiceps, respectively. We found that tapeworms in 2 cases (Cases 2 and 3) yielded exactly the same sequences between them, which differed from those in Cases 1 and 4, suggesting intra-species variation in tapeworms. These taeniasis cases by T. saginata infection in this study, which occurred within a limited time period and region, suggest the possibility of a mini-outbreak. This study highlights the need for further epidemiological investigation of potentially overlooked cases of T. saginata infection in Korea.

Citations

Citations to this article as recorded by

The prevalence and molecular detection of bovine cysticercosis and its impact on slaughtered cattle in Egypt
Khaled Mohamed El-Dakhly, Shaimaa Ahmed Hany, Waleed M. Arafa, Omima Ramadan Abdel-Fatah, Nasser Sayed Abdel-Atty, El-Shaymaa El-Nahass
Journal of Parasitic Diseases.2023; 47(3): 527. CrossRef

6,333 View
130 Download
Crossref

Original Articles

Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells: Pu Reum Sun, Fei Fei Gao, Hei Gwon Choi, Wei Zhou, Jae-Min Yuk, Jaeyul Kwon, Young-Ha Lee, Guang-Ho Cha; Korean J Parasitol 2019;57(2):83-92.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.83

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.

Citations

Citations to this article as recorded by

Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
Journal of Microbiology.2025; 63(2): e2409001. CrossRef
MjTX-II, a Lys49-PLA2 from Bothrops moojeni snake venom, restricts Toxoplasma gondii infection via ROS and VEGF regulation
Samuel Cota Teixeira, Thales Alves de Melo Fernandes, Guilherme de Souza, Alessandra Monteiro Rosini, Aryani Felixa Fajardo Martínez, Angelica Oliveira Gomes, Rosiane Nascimento Alves, Daiana Silva Lopes, Maria Vitoria da Silva, Emidio Beraldo-Neto, Patrí
Chemico-Biological Interactions.2025; 409: 111417. CrossRef
High-Throughput Repurposing Screen Reveals Compounds with Activity against Toxoplasma gondii Bradyzoites
Taher Uddin, Jing Xia, Yong Fu, Case W. McNamara, Arnab K. Chatterjee, L. David Sibley
ACS Infectious Diseases.2025; 11(3): 600. CrossRef
In Vitro Inhibitory Activity of Corilagin and Punicalagin Against Toxoplasma gondii and Their Mechanism(s) of Action
Nicole T. Green-Ross, Homa Nath Sharma, Audrey Napier, Boakai K. Robertson, Robert L. Green, Daniel A. Abugri
Antibiotics.2025; 14(4): 336. CrossRef
Metabolic changes in Toxoplasma gondii -infected host cells measured by autofluorescence imaging
Gina M. Gallego-López, Emmanuel Contreras Guzman, Danielle E. Desa, Laura J. Knoll, Melissa C. Skala, Anita A. Koshy
mBio.2024;[Epub] CrossRef
BjussuLAAO-II, an l-amino acid oxidase from Bothrops jararacussu snake venom, impairs Toxoplasma gondii infection in human trophoblast cells and villous explants from the third trimester of pregnancy
Thales Alves de Melo Fernandes, Samuel Cota Teixeira, Tássia Rafaela Costa, Alessandra Monteiro Rosini, Guilherme de Souza, Lorena Polloni, Bellisa de Freitas Barbosa, Marcelo José Barbosa Silva, Eloisa Amália Vieira Ferro, Veridiana de Melo Rodrigues Ávi
Microbes and Infection.2023; 25(6): 105123. CrossRef
DNA double-strand breaks in the Toxoplasma gondii-infected cells by the action of reactive oxygen species
Haohan Zhuang, Chaoqun Yao, Xianfeng Zhao, Xueqiu Chen, Yimin Yang, Siyang Huang, Lingtao Pan, Aifang Du, Yi Yang
Parasites & Vectors.2020;[Epub] CrossRef

8,864 View
194 Download
7 Web of Science
Crossref

Modulated Gene Expression of Toxoplasma gondii Infected Retinal Pigment Epithelial Cell Line (ARPE-19) via PI3K/Akt or mTOR Signal Pathway: Wei Zhou, Juan-Hua Quan, Fei-Fei Gao, Hassan Ahmed Hassan Ahmed Ismail, Young-Ha Lee, Guang-Ho Cha; Korean J Parasitol 2018;56(2):135-145.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.135

Abstract
PDF

Due to the critical location and physiological activities of the retinal pigment epithelial (RPE) cell, it is constantly subjected to contact with various infectious agents and inflammatory mediators. However, little is known about the signaling events in RPE involved in Toxoplasma gondii infection and development. The aim of the study is to screen the host mRNA transcriptional change of 3 inflammation-related gene categories, PI3K/Akt pathway regulatory components, blood vessel development factors and ROS regulators, to prove that PI3K/Akt or mTOR signaling pathway play an essential role in regulating the selected inflammation-related genes. The selected genes include PH domain and leucine- richrepeat protein phosphatases (PHLPP), casein kinase2 (CK2), vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), glutamate-cysteine ligase (GCL), glutathione S-transferase (GST), and NAD(P)H: quinone oxidoreductase (NQO1). Using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), we found that T. gondii up-regulates PHLPP2, CK2β, VEGF, GCL, GST, and NQO1 gene expression levels, but down-regulates PHLPP1 and PEDF mRNA transcription levels. PI3K inhibition and mTOR inhibition by specific inhibitors showed that most of these host gene expression patterns were due to activation of PI3K/Akt or mTOR pathways with some exceptional cases. Taken together, our results reveal a new molecular mechanism of these gene expression change dependent on PI3K/Akt or mTOR pathways and highlight more systematical insight of how an intracellular T. gondii can manipulate host genes to avoid host defense.

Citations

Citations to this article as recorded by

PTEN regulation in virus-associated cancers
Shaian Tavakolian, Zahra Shokati Eshkiki, Abolfazl Akbari, Ebrahim Faghihloo, Seidamir Pasha Tabaeian
Pathology - Research and Practice.2025; 266: 155749. CrossRef
MjTX-II, a Lys49-PLA2 from Bothrops moojeni snake venom, restricts Toxoplasma gondii infection via ROS and VEGF regulation
Samuel Cota Teixeira, Thales Alves de Melo Fernandes, Guilherme de Souza, Alessandra Monteiro Rosini, Aryani Felixa Fajardo Martínez, Angelica Oliveira Gomes, Rosiane Nascimento Alves, Daiana Silva Lopes, Maria Vitoria da Silva, Emidio Beraldo-Neto, Patrí
Chemico-Biological Interactions.2025; 409: 111417. CrossRef
Echinococcus multilocularis protoscoleces enhance glycolysis to promote M2 Macrophages through PI3K/Akt/mTOR Signaling Pathway
Tao Zhang, Yaogang Zhang, Zihan Yang, Yuan Jiang, Li Sun, Dengliang Huang, Meiyuan Tian, Yinhong Shen, Jun Deng, Jing Hou, Yanyan Ma
Pathogens and Global Health.2023; 117(4): 409. CrossRef
The interplay between toxoplasmosis and host miRNAs: Mechanisms and consequences
Ahmed S. Doghish, Mohamed A. Ali, Mahmoud A. Elrebehy, Hend H. Mohamed, Reda Mansour, Aml Ghanem, Ahmed Hassan, Mohammed S. Elballal, Ola Elazazy, Ahmed E. Elesawy, Sherif S. Abdel Mageed, Yara A. Nassar, Osama A. Mohammed, Ahmed I. Abulsoud
Pathology - Research and Practice.2023; 250: 154790. CrossRef
Mammalian Target of Rapamycin Inhibitor Rapamycin Alleviates 7-Ketocholesterol Induced Inflammatory Responses and Vascular Endothelial Growth Factor Elevation by Regulating MAPK Pathway in Human Retinal Pigment Epithelium Cells
Lin Yang, Peng Yu, Mei Chen, Bo Lei
Journal of Ocular Pharmacology and Therapeutics.2022; 38(2): 189. CrossRef
The host mTOR pathway and parasitic diseases pathogenesis
Sajad Rashidi, Reza Mansouri, Mohammad Ali-Hassanzadeh, Zahra Mojtahedi, Reza Shafiei, Amir Savardashtaki, Nasrin Hamidizadeh, Mohammadreza Karimazar, Paul Nguewa, Raúl Manzano-Román
Parasitology Research.2021; 120(4): 1151. CrossRef
Upregulation of PEDF Predicts a Poor Prognosis and Promotes Esophageal Squamous Cell Carcinoma Progression by Modulating the MAPK/ERK Signaling Pathway
Zui Chen, Di Che, Xiaoqiong Gu, Jiamin Lin, Jing Deng, Ping Jiang, Kaixiong Xu, Banglao Xu, Ting Zhang
Frontiers in Oncology.2021;[Epub] CrossRef
Modulation of the mTOR pathway plays a central role in dendritic cell functions after Echinococcus granulosus antigen recognition
Christian Rodriguez Rodrigues, María Celeste Nicolao, Maia Chop, Natalia Plá, Mora Massaro, Julia Loos, Andrea C. Cumino
Scientific Reports.2021;[Epub] CrossRef
Ripasudil alleviated the inflammation of RPE cells by targeting the miR-136-5p/ROCK/NLRP3 pathway
Zhao Gao, Qiang Li, Yunda Zhang, Xiaohong Gao, Haiyan Li, Zhigang Yuan
BMC Ophthalmology.2020;[Epub] CrossRef
H3 relaxin protects against calcium oxalate crystal‐induced renal inflammatory pyroptosis
Jiannan Liu, Kelaier Yang, Yinshan Jin, Yadong Liu, Yaodong Chen, Xiaohui Zhang, Shiliang Yu, Erlin Song, Song Chen, Jingbo Zhang, Guanhua Jing, Ruihua An
Cell Proliferation.2020;[Epub] CrossRef
Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
Pu Reum Sun, Fei Fei Gao, Hei Gwon Choi, Wei Zhou, Jae-Min Yuk, Jaeyul Kwon, Young-Ha Lee, Guang-Ho Cha
The Korean Journal of Parasitology.2019; 57(2): 83. CrossRef
Simultaneous Ribosome Profiling of Human Host Cells Infected with Toxoplasma gondii
Michael J. Holmes, Premal Shah, Ronald C. Wek, William J. Sullivan, Ira J. Blader
mSphere.2019;[Epub] CrossRef

11,196 View
182 Download
12 Web of Science
Crossref

Pyruvate Protects Giardia Trophozoites from Cysteine-Ascorbate Deprived Medium Induced Cytotoxicity: Dibyendu Raj, Punam Chowdhury, Rituparna Sarkar, Yumiko Saito-Nakano, Keinosuke Okamoto, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly; Korean J Parasitol 2018;56(1):1-9.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.1

Giardia lamblia, an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia. In the present study, we have examined the effects of extracellular addition of pyruvate, during oxidative stress generated from cysteine-ascorbate deprivation in culture media on DNA damage in Giardia. The intracellular pyruvate concentrations at several time points were measured in the trophozoites during stress. Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. The exogenous addition of a physiologically relevant concentration of pyruvate to trophozoites suspension was shown to attenuate the rate of ROS generation. We have demonstrated that Giardia protects itself from destructive consequences of ROS by maintaining the intracellular pyruvate concentration. Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair.

Citations

Citations to this article as recorded by

A myeloid leukemia factor homolog is involved in tolerance to stresses and stress-induced protein metabolism in Giardia lamblia
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Biology Direct.2023;[Epub] CrossRef
Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate
Yana Shaulov, Chikako Shimokawa, Meirav Trebicz-Geffen, Shruti Nagaraja, Karen Methling, Michael Lalk, Lea Weiss-Cerem, Ayelet T. Lamm, Hajime Hisaeda, Serge Ankri, William A. Petri
PLOS Pathogens.2018; 14(10): e1007295. CrossRef

9,130 View
204 Download
2 Web of Science
Crossref

Chigger Mite (Acari: Trombiculidae) Survey of Rodents in Shandong Province, Northern China: Xiao-Dan Huang, Peng Cheng, Yu-Qiang Zhao, Wen-Juan Li, Jiu-Xu Zhao, Hong-Mei Liu, Jing-Xuan Kou, Mao-Qing Gong; Korean J Parasitol 2017;55(5):555-559.
Published online October 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.5.555

Abstract
PDF

Chigger mites are parasites of rodents and other vertebrates, invertebrates, and other arthropods, and are the only vectors of scrub typhus, in addition to other zoonoses. Therefore, investigating their distribution, diversity, and seasonal abundance is important for public health. Rodent surveillance was conducted at 6 districts in Shandong Province, northern China (114-112°E, 34-38°N), from January to December 2011. Overall, 225/286 (78.7%) rodents captured were infested with chigger mites. A total of 451 chigger mites were identified as belonging to 5 most commonly collected species and 3 genera in 1 family. Leptotrombidium scutellare and Leptotrombidium intermedia were the most commonly collected chigger mites. L. scutellare (66.2%, 36.7%, and 49.0%) was the most frequently collected chigger mite from Apodemus agrarius, Rattus norvegicus, and Microtus fortis, respectively, whereas L. intermedia (61.5% and 63.2%) was the most frequently collected chigger mite from Cricetulus triton and Mus musculus, respectively. This study demonstrated a relatively high prevalence of chigger mites that varied seasonally in Shandong Province, China.

Citations

Citations to this article as recorded by

Spatiotemporal evolution and impacts of environment on scrub typhus in northern China, 2006–2019
Ting Li, Xianjun Wang, Yamei Wang, Chenxin Gu, Liping Yang
Journal of Global Health.2025;[Epub] CrossRef
Predicting the potential distribution areas of Leptotrombidium rubellum under current and future climate change
Qunzheng Mu, Fengfeng Li, Wenyu Li, Xiaoxia Wang, Mingyuan Tang, Kehan Chen, Yihao Jiang, Jingqi Liu, Shirong Zhang, Qiyong Liu, Chuan Wang
Frontiers in Public Health.2025;[Epub] CrossRef
Variation in Small Mammal Species Composition and the Occurrence of Parasitic Mites in Two Landscapes in a Scrub Typhus Endemic Region of Western Yunnan Province, China
Yun‐Yan Luo, Jia‐Xiang Yin, Zong‐Ti Shao, Zeng‐Kan Liu, Shou‐Qin Yin, Jiang‐Li Lu, Jin‐Chun Li, Rong Wei, Alan Frederick Geater
Ecology and Evolution.2025;[Epub] CrossRef
Explorational analysis of the abundance and prevalence of chigger and gamasid mites parasitic on small mammals in Vietnam
Anastasia A Antonovskaia, Evgeny P Altshuler, Alexander E Balakirev, Yuliya V Lopatina, Janet Foley
Journal of Medical Entomology.2024; 61(4): 925. CrossRef
Edifications on Indirect IgM ELISA and Immunofluorescence assay (IFA) of scrub typhus in humans and erudition of Orientia tsutsugamushi in the vector of cohabiting rodents
Shubham Patil, Archana Patil, Sandip Chaudhari, Shilpshri Shinde, Sunil Kolte, Waqar Khan, Nitin Kurkure
Journal of Vector Borne Diseases.2023; 60(3): 244. CrossRef
Epidemiological and clinical characteristics of scrub typhus in northern Fujian, China, from 2015 to 2019
Jin Huang, Kaixiang Deng, Jiawei Chen, Meiquan Zhang
BMC Infectious Diseases.2023;[Epub] CrossRef
Factors influencing the presence of parasitic trombiculids on red-backed voles (Myodes gapperi) in a temperate archipelago
Elyse C. Mallinger, Erik R. Olson, Geoffrey P. Vincent, Julie Van Stappen, Timothy R. Van Deelen
Canadian Journal of Zoology.2022; 100(9): 539. CrossRef
SFTSV infection in rodents and their ectoparasitic chiggers
Xiao-Lan Gu, Wen-Qing Su, Chuan-Min Zhou, Li-Zhu Fang, Ke Zhu, Dong-Qiang Ma, Fa-Chun Jiang, Ze-Min Li, Dan Li, Shu-Hui Duan, Qiu-Ming Peng, Rui Wang, Yuan Jiang, Hui-Ju Han, Xue-Jie Yu, Masayuki Saijo
PLOS Neglected Tropical Diseases.2022; 16(8): e0010698. CrossRef
Infestation and distribution of chigger mites on Confucian white-bellied rat (Niviventer confucianus) in Southwest China
Yan-Ling Chen, Xian-Guo Guo, Wen-Yu Song, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Wen-Ge Dong, Xiao-Bin Huang
Biologia.2022; 78(3): 727. CrossRef
A dataset of distribution and diversity of blood-sucking mites in China
Fan-Fei Meng, Qiang Xu, Jin-Jin Chen, Yang Ji, Wen-Hui Zhang, Zheng-Wei Fan, Guo-Ping Zhao, Bao-Gui Jiang, Tao-Xing Shi, Li-Qun Fang, Wei Liu
Scientific Data.2021;[Epub] CrossRef
Infestation and Related Ecology of Chigger Mites on the Asian House Rat (Rattus tanezumi) in Yunnan Province, Southwest China
Fan Ding, Wen-Li Jiang, Xian-Guo Guo, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Rong Xiang
The Korean Journal of Parasitology.2021; 59(4): 377. CrossRef
Orientia tsutsugamushi dynamics in vectors and hosts: ecology and risk factors for foci of scrub typhus transmission in northern Thailand
Ivo Elliott, Neeranuch Thangnimitchok, Kittipong Chaisiri, Tri Wangrangsimakul, Piangnet Jaiboon, Nicholas P. J. Day, Daniel H. Paris, Paul N. Newton, Serge Morand
Parasites & Vectors.2021;[Epub] CrossRef
A Report of Chigger Mites on the Striped Field Mouse, Apodemus agrarius, in Southwest China
Yan-Ling Chen, Xian-Guo Guo, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Xiao-Bin Huang, Ti-Jun Qian
The Korean Journal of Parasitology.2021; 59(6): 625. CrossRef
Identification of Trombiculid Chigger Mites Collected on Rodents from Southern Vietnam and Molecular Detection of Rickettsiaceae Pathogen
Minh Doan Binh, Sinh Cao Truong, Dong Le Thanh, Loi Cao Ba, Nam Le Van, Binh Do Nhu
The Korean Journal of Parasitology.2020; 58(4): 445. CrossRef
Bimonthly surveillance of wild rodents and chigger mites in urban parks in Daegu from 2018 to 2019
Yeong Bu Kim, Moon Bo Choi, Ohseok Kwon
Entomological Research.2020; 50(12): 609. CrossRef
Distribution of Orientia tsutsugamushi in rodents and mites collected from Central India
Batul Akhunji, Ruchi Bhate, Nilesh Pansare, S. P. Chaudhari, Waqar Khan, N. V. Kurkure, S. W. Kolte, S. B. Barbuddhe
Environmental Monitoring and Assessment.2019;[Epub] CrossRef
Dermoscopy confirmed Trombiculidae larva infestation in Turkey
Tamer I. Kaya, Mustafa A. Yilmaz
International Journal of Dermatology.2019;[Epub] CrossRef
Occurrence rates of wild rodent hosts and chigger mites and the infection rate ofOrientia tsutsugamushiin the central region of South Korea from 2015 to 2018
Moon Bo Choi, Yeongbu Kim, Da Young Kim, Ohseok Kwon
Entomological Research.2019; 49(8): 374. CrossRef
Analysis of epidemiological characteristics of four natural-focal diseases in Shandong Province, China in 2009-2017: A descriptive analysis
Rui Chen, Zengqiang Kou, Liuchen Xu, Jie Cao, Ziwei Liu, Xiaojing Wen, Zhiyu Wang, Hongling Wen, Isaac Chun-Hai Fung
PLOS ONE.2019; 14(8): e0221677. CrossRef

8,986 View
160 Download
21 Web of Science
Crossref

Brief Communication

Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis: In-Wook Choi, Hwang-Yong Kim, Juan-Hua Quan, Jae-Gee Ryu, Rubing Sun, Young-Ha Lee; Korean J Parasitol 2015;53(5):641-645.
Published online October 29, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.5.641

Abstract
PDF

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

Citations

Citations to this article as recorded by

Isolation and molecular identification of liver fluke cercariae in freshwater snails of Chaharmahal and Bakhtiari province, Iran
Bijan Hosseinpour Aghaei, Nadia Taiefi Nasrabadi, Yaser Pirali Kheirabadi, Seyed Shapoor Reza Shojaei
Molluscan Research.2024; 44(1): 84. CrossRef
Morphological and molecular identification of lymnaeid snail and trematodes cercariae in different water bodies in Perak, Malaysia
Nazir Ahmad Tookhy, Nur Mahiza Md Isa, Rozaihan Mansor, Yasmin Abd Rahaman, Nur Indah Ahmad, Dung Thi Bui, Lokman Hakim Idris, Noor Hazfalinda Hamzah, Norhadila Zulkifli
Parasitology Research.2023; 122(7): 1475. CrossRef
Green vegetable juice as a potential source of human fascioliasis in Korea
Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
One Health.2022; 15: 100441. CrossRef
Phylogenetic Characteristics of Fasciola hepatica Isolated from a Korean Patient
Mi Jin Jeong, Jae Kyun Park, Hak Sun Yu
The Korean Journal of Parasitology.2022; 60(5): 367. CrossRef
A Descriptive Study of Human Fascioliasis in Qaemshahr, Mazandaran Province, Iran: Its Prevalence and Risk Factors
Lotfollah Davoodi, Azadeh Mizani, Roya Najafi-Vosough, Saeed Hosseini Teshnizi, afsane amouei, Mousa Motavallihaghi, Hamideh Izadyar, Fateme Amuei, Sara Pourhaghighi, Seyed Reza Mirbadie, Eissa Soleymani
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef
A Review ofOenanthe javanica(Blume) DC. as Traditional Medicinal Plant and Its Therapeutic Potential
Chuan-li Lu, Xiu-fen Li
Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1. CrossRef
Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures
S. Mas-Coma, M. D. Bargues, M. A. Valero
Parasitology.2018; 145(13): 1665. CrossRef

9,962 View
107 Download
8 Web of Science
Crossref

Original Articles

Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia: Mudyawati Kamaruddin, Masaharu Tokoro, Md. Moshiur Rahman, Shunsuke Arayama, Anggi P.N. Hidayati, Din Syafruddin, Puji B.S. Asih, Hisao Yoshikawa, Ei Kawahara; Korean J Parasitol 2014;52(5):471-478.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.471

Abstract
PDF

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.

Citations

Citations to this article as recorded by

18S rRNA gene metabarcoding for investigation of gastrointestinal parasite diversity in great cormorants
Subin Lee, Badriah Alkathiri, Chang Hyeon Lee, Heon Woo Lee, Dong-Hyuk Jeong, Ju Yeong Kim, Seongjun Choe, Seung-Hun Lee
Scientific Reports.2025;[Epub] CrossRef
Spatial composition differences and diversity of gut eukaryotes in white-lipped deer in Xizang, China
Yuangang Yang, Wei Luo, Peng Luo, Mingkun He, Feng Jiang, Jianli Xiong, Zhangqiang You
Zoologia (Curitiba).2025;[Epub] CrossRef
Development of a novel one-tube LAMP-CRISPR/Cas12b technique for detecting Pentatrichomonas hominis
Qing-Xin Fan, Jin-Long Wang, Yao Liang, Yu-Xuan Wang, Han-Dan Xiao, Xing-Quan Zhu, Qing Liu
Microchemical Journal.2025; 215: 114488. CrossRef
Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single‐Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as ‘Non‐Pathogenic’
Christen Rune Stensvold
APMIS.2025;[Epub] CrossRef
Prevalence of intestinal trichomonads in captive non-human primates in China
Ping-Ping Ma, Yang Zou, Wen-Jie Mu, Yue-Yue Zhang, Ya-Qi Li, Zhong-Li Liu, Long Zhang, Li-Xian Chen, Guo-Hua Liu, Shuai Wang
Parasite.2024; 31: 19. CrossRef
Development of an LFD-RPA Assay for Rapid Detection of Pentatrichomonas hominis Infection in Dogs
Yao Rong, Xichen Zhang, Xuejiao Chen, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Taotao Yue, Hongbo Zhang, Xiaofei Zhou, Nan Zhang
Current Issues in Molecular Biology.2023; 45(11): 9252. CrossRef
Prevalence and molecular characterization of Pentatrichomonas hominis in Siberian tigers (Panthera tigris altaica) in northeast China
Hongbo ZHANG, Nan ZHANG, Pengtao GONG, Shuqin CHENG, Xiaocen WANG, Xin LI, Zhijun HOU, Chang LIU, Tianqi BI, Bobo WANG, Yidan CHENG, Jianhua LI, Xichen ZHANG
Integrative Zoology.2022; 17(4): 543. CrossRef
Pentatrichomonas hominis and other intestinal parasites in school-aged children: coproscopic survey
Sarah Mohamed Abdo, Marwa Mohamed Ibrahim Ghallab, Nagwa Mohammed Elhawary, Heba Elhadad
Journal of Parasitic Diseases.2022; 46(3): 896. CrossRef
Occurrence and Genetic Diversity of the Zoonotic Enteric Protozoans and Enterocytozoon bieneusi in Père David’s Deer (Elaphurus davidianus) from Beijing, China
Peiyang Zhang, Qingxun Zhang, Shuyi Han, Guohui Yuan, Jiade Bai, Hongxuan He
Pathogens.2022; 11(11): 1223. CrossRef
Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand
Aongart Mahittikorn, Ruenruetai Udonsom, Khuanchai Koompapong, Rachatawan Chiabchalard, Chantira Sutthikornchai, Preeyaporn Monatrakul Sreepian, Hirotake Mori, Supaluk Popruk
BMC Veterinary Research.2021;[Epub] CrossRef
Prevalence of Tetratrichomonas buttreyi and Pentatrichomonas hominis in yellow cattle, dairy cattle, and water buffalo in China
Wen-Chao Li, Jia-min Huang, Zhui Fang, Qi Ren, Li Tang, Zhen-zhen Kan, Xin-chao Liu, You-fang Gu
Parasitology Research.2020; 119(2): 637. CrossRef
Protozoan genital invasions caused by the representatives of trichomonas and giardia
Pavlo V. Fedorych, Gennadiy I. Mavrov, Tetiana V. Osinska, Yuliia V. Shcherbakova
Wiadomości Lekarskie.2020; 73(2): 380. CrossRef
Molecular prevalence of trichomonad species from pet shop puppies and kittens in Japan
Naoyuki Itoh, Yuko Iijima, Isao Ogura, Natsumi Yonekura, Satoshi Kameshima, Yuya Kimura
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Three monophyletic clusters in Retortamonas species isolated from vertebrates
Joko Hendarto, Tetsushi Mizuno, Anggi P.N. Hidayati, Ismail E. Rozi, Puji B.S. Asih, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Masaharu Tokoro
Parasitology International.2019; 69: 93. CrossRef
Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia
Takahiro Matsumura, Joko Hendarto, Tetsushi Mizuno, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Taro Nishimura, Masaharu Tokoro
Tropical Medicine and Health.2019;[Epub] CrossRef
Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid
Chao Lin, Furong Ying, Yanan Lai, Xiaolong Li, Xiangyang Xue, Tieli Zhou, Dongwei Hu
BMC Infectious Diseases.2019;[Epub] CrossRef
A survey of gastrointestinal parasites of goats in a goat market in Kathmandu, Nepal
Tirth Raj Ghimire, Namita Bhattarai
Journal of Parasitic Diseases.2019; 43(4): 686. CrossRef
High prevalence of Pentatrichomonas hominis infection in gastrointestinal cancer patients
Nan Zhang, Hongbo Zhang, Yanhui Yu, Pengtao Gong, Jianhua Li, Ziyi Li, Ting Li, Zhanjie Cong, Chunying Tian, Xiaofeng Liu, Xiuyan Yu, Xichen Zhang
Parasites & Vectors.2019;[Epub] CrossRef
Meta-taxonomic analysis of prokaryotic and eukaryotic gut flora in stool samples from visceral leishmaniasis cases and endemic controls in Bihar State India
Rachael Lappan, Cajsa Classon, Shashi Kumar, Om Prakash Singh, Ricardo V. de Almeida, Jaya Chakravarty, Poonam Kumari, Sangeeta Kansal, Shyam Sundar, Jenefer M. Blackwell, Guy Caljon
PLOS Neglected Tropical Diseases.2019; 13(9): e0007444. CrossRef
Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China
Wen Chao Li, Kai Wang, Youfang Gu
Parasites & Vectors.2018;[Epub] CrossRef
Simplicimonas- like DNA in vaginal swabs of cows and heifers cross-reacting in the real-time PCR for T. foetus
Caroline F. Frey, Norbert Müller, Norbert Stäuber, Nelson Marreros, Larissa Hofmann, Brigitte Hentrich, Gaby Hirsbrunner
Veterinary Parasitology.2017; 237: 30. CrossRef
Report of the first clinical case of intestinal trichomoniasis caused by Tritrichomonas foetus in a cat with chronic diarrhoea in Brazil
Aline S. Hora, Samantha I. Miyashiro, Fabiana C. Cassiano, Paulo E. Brandão, Archivaldo Reche-Junior, Hilda F. J. Pena
BMC Veterinary Research.2017;[Epub] CrossRef
Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay
Douglas Chan, Joel Barratt, Tamalee Roberts, Owen Phillips, Jan Šlapeta, Una Ryan, Deborah Marriott, John Harkness, John Ellis, Damien Stark
Veterinary Parasitology.2016; 227: 42. CrossRef
Human Blastocystis subtyping with subtype-specific primers developed from unique sequences of the SSU rRNA gene
Hisao Yoshikawa, Ayana Iwamasa
Parasitology International.2016; 65(6): 785. CrossRef
Molecular and morphological diversity of the genus Hypotrichomonas (Parabasalia: Hypotrichomonadida), with descriptions of six new species
Vít Céza, Tomáš Pánek, Pavla Smejkalová, Ivan Čepička
European Journal of Protistology.2015; 51(2): 158. CrossRef

13,790 View
153 Download
22 Web of Science
Crossref

A New Species of Chigger Mite (Acari: Trombiculidae) from Rodents in Southwest China: Tian-Guang Ren, Xian-Guo Guo, Dao-Chao Jin, Dian Wu, Quinn E. Fletcher; Korean J Parasitol 2014;52(1):63-67.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.63

Abstract
PDF

This paper describes a new species of chigger mite (Acari: Trombiculidae), Gahrliepia cangshanensis n. sp., from rodents in southwest China. The specimens were collected from Yunnan red-backed voles, Eothenomys miletus (Thomas, 1914), and a Chinese white-bellied rat, Niviventer confucianus (Milne-Edwards, 1871) in Yunnan Province. The new species is unique mainly in its number of dorsal setae (n=21), and it has the following features: fT (formula of palpotarsus)=4B (B=branched), fp (formula of palpal seta)=B/N/N/N/B (N=naked), a broad tongue-shaped scutum with an almost straight posterior margin, and 17 PPLs (posterior posterolateral seta) with a length of 36-43 ?m. This chigger mite may also infect other rodent hosts and may be distributed in other localities.

Citations

Citations to this article as recorded by

Infestation, community structure, seasonal fluctuation and climate-driven dynamics of mites on small mammals at a focus of scrub typhus in southwest China
Peng-Wu Yin, Yan Lv, Xian-Guo Guo, Wen-Yu Song, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ya-Fei Zhao, Wen-Ge Dong, Dao-Chao Jin
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Species abundance distribution and ecological niches of chigger mites on small mammals in Yunnan province, southwest China
Pei-Ying Peng, Xian-Guo Guo, Dao-Chao Jin, Wen-Ge Dong, Ti-Jun Qian, Feng Qin, Zhi-Hua Yang
Biologia.2017; 72(9): 1031. CrossRef
An updated distribution and hosts: trombiculid mites (Acari: Trombidiformes) associated with small mammals in Yunnan Province, southwest China
Pei-Ying Peng, Xian-Guo Guo, Tian-Guang Ren, Wen-Ge Dong, Wen-Yu Song
Parasitology Research.2016; 115(5): 1923. CrossRef

10,145 View
84 Download
6 Web of Science
Crossref

Entamoeba histolytica Induces Cell Death of HT29 Colonic Epithelial Cells via NOX1-Derived ROS: Kyeong Ah Kim, Ju Young Kim, Young Ah Lee, Arim Min, Young Yil Bahk, Myeong Heon Shin; Korean J Parasitol 2013;51(1):61-68.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.61

Abstract
PDF

Entamoeba histolytica, which causes amoebic colitis and occasionally liver abscess in humans, is able to induce host cell death. However, signaling mechanisms of colon cell death induced by E. histolytica are not fully elucidated. In this study, we investigated the signaling role of NOX in cell death of HT29 colonic epithelial cells induced by E. histolytica. Incubation of HT29 cells with amoebic trophozoites resulted in DNA fragmentation that is a hallmark of apoptotic cell death. In addition, E. histolytica generate intracellular reactive oxygen species (ROS) in a contact-dependent manner. Inhibition of intracellular ROS level with treatment with DPI, an inhibitor of NADPH oxidases (NOXs), decreased Entamoeba-induced ROS generation and cell death in HT29 cells. However, pan-caspase inhibitor did not affect E. histolytica-induced HT29 cell death. In HT29 cells, catalytic subunit NOX1 and regulatory subunit Rac1 for NOX1 activation were highly expressed. We next investigated whether NADPH oxidase 1 (NOX1)-derived ROS is closely associated with HT29 cell death induced by E. histolytica. Suppression of Rac1 by siRNA significantly inhibited Entamoeba-induced cell death. Moreover, knockdown of NOX1 by siRNA, effectively inhibited E. histolytica-triggered DNA fragmentation in HT29 cells. These results suggest that NOX1-derived ROS is required for apoptotic cell death in HT29 colon epithelial cells induced by E. histolytica.

Citations

Citations to this article as recorded by

Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2023; 61(4): 388. CrossRef
Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica
Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
The interaction betweenEntamoeba histolyticaand enterobacteria shed light on an ancient antibacterial response
Nancy Guillén
Cellular Microbiology.2019;[Epub] CrossRef
NOX4 activation is involved in ROS‐dependent Jurkat T‐cell death induced by Entamoeba histolytica
Young Ah Lee, Kyeong Ah Kim, Arim Min, Myeong Heon Shin
Parasite Immunology.2019;[Epub] CrossRef
O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death
Young Ah Lee, Arim Min, Myeong Heon Shin
Microbial Pathogenesis.2018; 123: 285. CrossRef
GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways
Ming‑Zhen Wang, Cheng‑Zhi Qiu, Wai‑Shi Yu, Yan‑Ta Guo, Chun‑Xiao Wang, Zhi‑Xiong Chen
Molecular Medicine Reports.2017;[Epub] CrossRef
Infection Strategies of Intestinal Parasite Pathogens and Host Cell Responses
Bruno M. Di Genova, Renata R. Tonelli
Frontiers in Microbiology.2016;[Epub] CrossRef
A whole-genome RNAi screen uncovers a novel role for human potassium channels in cell killing by the parasite Entamoeba histolytica
Chelsea Marie, Hans P. Verkerke, Dan Theodorescu, William A. Petri
Scientific Reports.2015;[Epub] CrossRef
Degradation of the Transcription Factors NF-κB, STAT3, and STAT5 Is Involved in Entamoeba histolytica-Induced Cell Death in Caco-2 Colonic Epithelial Cells
Kyeong Ah Kim, Arim Min, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2014; 52(5): 459. CrossRef
Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice
Rima Ramonaite
World Journal of Gastroenterology.2014; 20(35): 12533. CrossRef
Small GTPases of the Ras superfamily regulate intestinal epithelial homeostasis and barrier function via common and unique mechanisms
Alí Francisco Citalán-Madrid, Alexander García-Ponce, Hilda Vargas-Robles, Abigail Betanzos, Michael Schnoor
Tissue Barriers.2013; 1(5): e26938. CrossRef
The EhCPADH112 Complex of Entamoeba histolytica Interacts with Tight Junction Proteins Occludin and Claudin-1 to Produce Epithelial Damage
Abigail Betanzos, Rosario Javier-Reyna, Guillermina García-Rivera, Cecilia Bañuelos, Lorenza González-Mariscal, Michael Schnoor, Esther Orozco, Johanna M. Brandner
PLoS ONE.2013; 8(6): e65100. CrossRef

11,010 View
103 Download
Crossref

Articles from Symposium on Asian Taenia (October 2011, Osong, Korea)

Current Status of Taeniasis in Thailand: Malinee Thairungroj Anantaphruti; Korean J Parasitol 2013;51(1):37-42.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.37

Abstract
PDF

Taeniasis is prevalent in all regions of Thailand, except the South. Infections were more frequently found in males than females of any age from 7-83 years. Taenia saginata is the most common species throughout the country. Taenia asiatica was reported only in the province of Kanchanaburi in the Central region. Co-infections, with Taenia solium and T. asiatica or T. solium and T. saginata, were found. Hybridization between T. asiatica and T. saginata is evidence that co-infection is never found between these 2 species. Finding more than 1 worm in a single patient was not entirely rare. Genetic variation was found without correlation to its geographic distribution in T. saginata, whereas no variation was found in T. asiatica.

Citations

Citations to this article as recorded by

Nationwide spatial dynamics of taeniasis in Thailand: declining prevalence but shifting focus and One Health risk factors across 2008–2014
Pornphutthachat Sota, Kefyalew Addis Alene, Thitima Wongsaroj, Sirikachorn Tangkawattana, Archie C. A. Clements, Banchob Sripa
Parasites & Vectors.2025;[Epub] CrossRef
Assessment of knowledge and practices regarding taeniasis and cysticercosis in Pak Chong, Nakhon Ratchasima, Thailand: A cross-sectional study
Wuttipong Phumrattanaprapin, Nitima Tatiya-apiradee, Pattana Jantaban, Wiriya Mahikul, Gideon Zulu
PLOS ONE.2024; 19(7): e0307240. CrossRef
Current prevalence and geographic distribution of helminth infections in the parasitic endemic areas of rural Northeastern Thailand
Pongsakorn Martviset, Wansika Phadungsil, Kesara Na-Bangchang, Wiwat Sungkhabut, Tanutchamon Panupornpong, Parisa Prathaphan, Nattaya Torungkitmangmi, Salisa Chaimon, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Pichanee Watthanasiri, Amornrat
BMC Public Health.2023;[Epub] CrossRef
A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us?
Luis Enrique Jerez Puebla, Fidel A. Núñez Fernández, Jorge Fraga Nodarse, Raul Cordovi Prado, Iraís Atencio Millán, Iredys Cruz Rodríguez, Rigoberto Fimia Duarte, Marina del Carmen Sánchez Romero, Sahily de la Caridad Ortega Medina, Ubaldo del Risco, Liss
Food and Waterborne Parasitology.2021; 25: e00136. CrossRef
Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny
Parasites & Vectors.2020;[Epub] CrossRef
Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
Acta Tropica.2019; 198: 105075. CrossRef
Comment on “Epidemiological Survey on Porcine Cysticercosis in Nay Pyi Taw Area, Myanmar”
Maria Teresa Galán-Puchades, Màrius Vicent Fuentes
Journal of Veterinary Medicine.2016; 2016: 1. CrossRef
Epidemiology and genetic diversity of Taenia asiatica: a systematic review
Anita Ale, Bjorn Victor, Nicolas Praet, Sarah Gabriël, Niko Speybroeck, Pierre Dorny, Brecht Devleesschauwer
Parasites & Vectors.2014;[Epub] CrossRef
Current Status of Taeniasis in Thailand
Malinee Thairungroj Anantaphruti
The Korean Journal of Parasitology.2013; 51(1): 37. CrossRef
Genetic Diversity of Taenia asiatica from Thailand and Other Geographical Locations as Revealed by Cytochrome c Oxidase Subunit 1 Sequences
Malinee Thairungroj Anantaphruti, Urusa Thaenkham, Dorn Watthanakulpanich, Orawan Phuphisut, Wanna Maipanich, Tippayarat Yoonuan, Supaporn Nuamtanong, Somjit Pubampen, Surapol Sanguankiat
The Korean Journal of Parasitology.2013; 51(1): 55. CrossRef

10,390 View
83 Download
Crossref

Original Article

Molecular Variation and Distribution of Anopheles fluviatilis (Diptera: Culicidae) Complex in Iran: Saied Reza Naddaf, Mohammad Reza Razavi, Golnaz Bahramali; Korean J Parasitol 2010;48(3):231-236.
Published online September 16, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.3.231

Abstract
PDF

Anopheles fluviatilis James (Diptera: Culicidae) is one of the known malaria vectors in south and southeastern Iran. Earlier ITS2 sequences analysis of specimens from Iran demonstrated only a single genotype that was identical to species Y in India, which is also the same as species T. We identified 2 haplotypes in the An. fluviatilis populations of Iran based on differences in nucleotide sequences of D3 domain of the 28S locus of ribosomal DNA (rDNA). Comparison of sequence data from 44 Iranian specimens with those publicly available in the Genbank database showed that all of the 28S-D3 sequences from Kazeroun and Khesht regions in Fars Province were identical to the database entry representing species U in India. In other regions, all the individuals showed heterozygosity at the single nucleotide position, which identifies species U and T. It is argued that the 2 species may co-occur in some regions and hybridize; however, the heterozygosity in the 28S-D3 locus was not reflected in ITS2 sequences and this locus for all individuals was identical to species T. This study shows that in a newly diverged species, like members of An. fluviatilis complex, a single molecular marker may not be sufficiently discriminatory to identify all the taxa over a vast geographical area. In addition, other molecular markers may provide more reliable information for species discrimination.

Citations

Citations to this article as recorded by

Are members of the Anopheles fluviatilis complex conspecific?
Om P. Singh, Ankita Sindhania, Gunjan Sharma, Shobhna Mishra, Surya K. Sharma, Piyoosh K. Singh, Manoj K. Das
Acta Tropica.2021; 224: 106149. CrossRef
A detailed review of the mosquitoes (Diptera: Culicidae) of Iran and their medical and veterinary importance
Shahyad Azari-Hamidian, Behzad Norouzi, Ralph E. Harbach
Acta Tropica.2019; 194: 106. CrossRef
Spatial changes in the distribution of malaria vectors during the past 5 decades in Iran
A. Salahi-Moghaddam, A. Khoshdel, H. Dalaei, K. Pakdad, G.G. Nutifafa, M.M. Sedaghat
Acta Tropica.2017; 166: 45. CrossRef
An evaluation of the suitability of COI and COII gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (Diptera Culicidae)
Gang Wang, Chunxiao Li, Wei Zheng, Fenglin Song, Xiaoxia Guo, Zhonghua Wu, Peng Luo, Yongyao Yang, Lei He, Tongyan Zhao
Mitochondrial DNA Part A.2017; 28(5): 769. CrossRef
An integrative approach to species discrimination in the Anagrus atomus group sensu stricto (Hymenoptera: Mymaridae), with a description of a new species
Francesco Nugnes, Umberto Bernardo, Gennaro Viggiani
Systematics and Biodiversity.2017; 15(6): 582. CrossRef
Molecular Phylogenetic Analysis of the SubgeneraAnophelesandCellia(Diptera: Culicidae) Based on Nuclear Ribosomal Sequences
G. Wang, C. Li, X. Guo, D. Xing, Y. Dong, T. Zhao
African Entomology.2014; 22(3): 660. CrossRef
Molecular phylogenetic analysis of Anopheles and Cellia subgenus anophelines (Diptera: Culicidae) in temperate and tropical regions of Iran
Saber Gholizadeh, Navid Dinparast Djadid, Behzad Nouroozi, Mojtaba Bekmohammadi
Acta Tropica.2013; 126(1): 63. CrossRef
Mosquito fauna (Diptera: Culicidae) of the Iranian islands in the Persian Gulf II. Greater Tonb, Lesser Tonb and Kish Islands
M. Khoobdel, S. Azari-Hamidian, A.A. Hanafi-Bojd
Journal of Natural History.2012; 46(31-32): 1939. CrossRef
Malaria elimination trend from a hypo-endemic unstable active focus in southern Iran: predisposing climatic factors
M D Moemenbellah-Fard, V Saleh, O Banafshi, T Dabaghmanesh
Pathogens and Global Health.2012; 106(6): 358. CrossRef
Spatio—temporal distribution of malaria vectors (Diptera: Culicidae) across different climatic zones of Iran
Ahmad Ali Hanafi-Bojd, Shahyad Azari-Hamidian, Vatandoost Hassan, Charrahy Zabihollah
Asian Pacific Journal of Tropical Medicine.2011; 4(6): 498. CrossRef