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Brief Communication

Microscopic findings of host–parasite interaction between the Korean rockfish, Sebastes schlegelii, and the monogenean ectoparasite, Microcotyle sebastis
Sang Phil Shin, Seokryel Kim
Parasites Hosts Dis 2024;62(4):490-496.
Published online November 22, 2024
DOI: https://doi.org/10.3347/PHD.24043
This study was conducted to examine the pathogenesis of gill degeneration in the Korean rockfish Sebastes schlegelii, infested with the monogenean ectoparasite, Microcotyle sebastis. We collected 30 Korean rockfish from a fish farm in Tongyeong-si, Gyeongsangnam-do, and examined them with light microscopy, scanning electron microscopy (SEM), and histopathology, in March 2018. The monogenean trematode, M. sebastis, was detected in 27 Korean rockfish (90%), with the intensity of infection being 31.7 per fish. The characteristic surface ultrastructures such as tegument with transverse striations, genital atrium, genital pore, and opisthaptor with numerous clamps were observed. The worms were firmly attached to the gill lamellae using clamps from the opisthaptor, causing gill damage and degeneration. The distal part of the lamellae was ruptured by the sclerites of the clamps. The histopathological examination revealed epithelial hypertrophy, hyperplasia, and occasionally fusion of the lamellae. These ultrastructural and histopathological findings provide some understanding of the pathogenesis of gill degeneration in the Korean rockfish infested with M. sebastis.
  • 1,880 View
  • 70 Download

Original Article

Population genetic analysis of Plasmodium vivax vir genes in Pakistan
Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo
Parasites Hosts Dis 2024;62(3):313-322.
Published online August 26, 2024
DOI: https://doi.org/10.3347/PHD.24036
Plasmodium vivax variant interspersed repeats (vir) refer to the key protein used for escaping the host immune system. Knowledge in the genetic variation of vir genes can be used for the development of vaccines or diagnostic methods. Therefore, we evaluated the genetic diversity of the vir genes of P. vivax populations of several Asian countries, including Pakistan, which is a malaria-endemic country experiencing a significant rise in malaria cases in recent years. We analyzed the genetic diversity and population structure of 4 vir genes (vir 4, vir 12, vir 21, and vir 27) in the Pakistan P. vivax population and compared these features to those of the corresponding vir genes in other Asian countries. In Pakistan, vir 4 (S=198, H=9, Hd=0.889, Tajima’s D value=1.12321) was the most genetically heterogenous, while the features of vir 21 (S=8, H=7, Hd=0.664, Tajima’s D value =-0.63763) and vir 27 (S =25, H =11, Hd =0.682, Tajima’s D value=-2.10836) were relatively conserved. Additionally, vir 4 was the most genetically diverse among Asian P. vivax populations, although within population diversity was low. Meanwhile, vir 21 and vir 27 among all Asian populations were closely related genetically. Our findings on the genetic diversity of vir genes and its relationships between populations in diverse geographical locations contribute toward a better understanding of the genetic characteristics of vir. The high level of genetic diversity of vir 4 suggests that this gene can be a useful genetic marker for understanding the P. vivax population structure. Longitudinal genetic diversity studies of vir genes in P. vivax isolates obtained from more diverse geographical areas are needed to better understand the function of vir genes and their use for the development of malaria control measures, such as vaccines.

Citations

Citations to this article as recorded by  Crossref logo
  • Genetic polymorphisms of merozoite surface protein-3α in Plasmodium vivax isolates from Pakistan
    Kim Oanh Nguyễn, Jung-Mi Kang, Tuấn Cường Võ, Hương Giang Lê, Seemab Akhtar, Thu Hằng Nguyễn, Đăng Thùy Dương Nguyễn, Minkyoung Cho, Sahib Gul Afridi, Byoung-Kuk Na
    Acta Tropica.2025; 272: 107904.     CrossRef
  • Genetic polymorphism of Duffy binding protein in Pakistan Plasmodium vivax isolates
    Đăng Thùy Dương Nguyễn, Tuấn Cường Võ, Kim Oanh Nguyễn, Hương Giang Lê, Jung-Mi Kang, Thu Hằng Nguyễn, Minkyoung Cho, Sahib Gul Afridi, Byoung-Kuk Na
    Acta Tropica.2024; 260: 107421.     CrossRef
  • 3,089 View
  • 78 Download
  • 2 Web of Science
  • Crossref

Brief Communication

Lophomonas blattarum-like organism in bronchoalveolar lavage from a pneumonia patient: current diagnostic scheme and polymerase chain reaction can lead to false-positive results
Moses Lee, Sang Mee Hwang, Jong Sun Park, Jae Hyeon Park, Jeong Su Park
Parasites Hosts Dis 2023;61(2):202-209.
Published online May 23, 2023
DOI: https://doi.org/10.3347/PHD.22107
Lophomonas blattarum is an anaerobic protozoan living in the intestine of cockroaches and house dust mites, with ultramicroscopic characteristics such as the presence of a parabasal body, axial filament, and absence of mitochondria. More than 200 cases of Lophomonas infection of the respiratory tract have been reported worldwide. However, the current diagnosis of such infection depends only on light microscopic morphological findings from respiratory secretions. In this study, we attempted to provide more robust evidence of protozoal infection in an immunocompromised patient with atypical pneumonia, positive for Lophomonas-like protozoal cell forms. A direct search of bronchoalveolar lavage fluid via polymerase chain reaction (PCR), transmission electron microscopy (TEM), and metagenomic next-generation sequencing did not prove the presence of protozoal infection. PCR results were not validated with sufficient rigor, while de novo assembly and taxonomic classification results did not confirm the presence of an unidentified pathogen. The TEM results implied that such protozoal forms in light microscopy are actually non-detached ciliated epithelial cells. After ruling out infectious causes, the patient’s final diagnosis was drug-induced pneumonitis. These findings underscore the lack of validation in the previously utilized diagnostic methods, and more evidence in the presence of L. blattarum is required to further prove its pathogenicity.

Citations

Citations to this article as recorded by  Crossref logo
  • Pulmonary infection by Lophomonas spp. and Aspergillus spp. in a B-cell acute lymphocytic leukemia patient from Chile
    Isabel Iturrieta-González, Enzo Moenen-Locoz, Alejandro Hidalgo, Fernando Vega, Johanna Cabrera, Carla Concha, Carolina Chahin, Javier Briones, Flery Fonseca-Salamanca
    Parasitology International.2025; 104: 102971.     CrossRef
  • A Reação em Cadeia da Polimerase (PCR) como estratégia de diagnóstico e monitoramento nas protozooses: uma revisão integrativa
    Luiza Valente Breda Libardi, Juliana Gouveia da Silva, Danieli Perini Alvares, Clara Fagundes Marely, Bruno Serafini Venturini, Guilherme Frinhani Bernardineli, Filipe Salume Lima Nogueira, Adelson Luiz Ferreira, Rodrigo Moraes, Priscila Pinto e Silva do
    Clinics Biopsychosocial.2025; 3(1): 58.     CrossRef
  • First Report of the Pulmonary Parasite Lophomonas blattarum in Ahvaz Province, Khuzestan: Case Report and Literature Review
    Mojtaba Aghaei, Hanieh Raji, Seyed Sobhan Bahreiny, Mohammad Amin Vahid, Najmaldin Saki
    Advanced Biomedical Research.2025;[Epub]     CrossRef
  • The diagnostic ambiguity for Polymerase Chain Reaction in the detection of Lophomonas blattarum
    Canan Doğu Tok, Funda Dogruman Al
    Middle Black Sea Journal of Health Science.2025; 11(4): 356.     CrossRef
  • First Molecular Evidence of Emerging Lophomonas Pathogen Among Patients Suspected of Having Pulmonary Tuberculosis in Mazandaran Province, Northern Iran
    Hossein Veisi, Abdollah Malakian, Mahdi Fakhar, Ali Sharifpour, Maryam Nakhaei, Mostafa Soleymani, Seyed Reza Mosave, Nader Ahangar, Seyed Hosien Seyedpour
    Acta Parasitologica.2024; 69(1): 1041.     CrossRef
  • Imaging patterns of Lophomonas blattarum infection in the respiratory tract: a registry-based analysis
    Amirmasoud Taheri, Mahdi Fakhar, Ali Sharifpour, Elhamsadat Banimostafavi, Sepideh SafaNavaei, Siavash Abedi, Hossein Mehravaran, Masoud Aliyali, Ahmad Shafahi, Asieh Delpzir
    BMC Infectious Diseases.2024;[Epub]     CrossRef
  • Case series of complicated bronchopulmonary lophomoniasis. Differential diagnosis of tuberculosis?
    Cristian Morán-Mariños, Juan Salas López, Felix Llanos-Tejada, Renato Casanova-Mendoza, Renzo Villanueva-Villegas, Antonella Chavez-Huamani, Kenneth G. Vargas-Ponce, Margaret Condori-Zevallos
    European Clinical Respiratory Journal.2024;[Epub]     CrossRef
  • Infliximab

    Reactions Weekly.2023; 1969(1): 197.     CrossRef
  • 4,331 View
  • 255 Download
  • 6 Web of Science
  • Crossref

Original Articles

Genetic Diversity of Ascaris in China Assessed Using Simple Sequence Repeat Markers
Chunhua Zhou, Shaoqing Jian, Weidong Peng, Min Li
Korean J Parasitol 2018;56(2):175-181.
Published online April 30, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.2.175
The giant roundworm Ascaris infects pigs and people worldwide and causes serious diseases. The taxonomic relationship between Ascaris suum and Ascaris lumbricoides is still unclear. The purpose of the present study was to investigate the genetic diversity and population genetic structure of 258 Ascaris specimens from humans and pigs from 6 sympatric regions in Ascaris-endemic regions of China using existing simple sequence repeat data. The microsatellite markers showed a high level of allelic richness and genetic diversity in the samples. Each of the populations demonstrated excess homozygosity (Ho<He, Fis>0). According to a genetic differentiation index (Fst=0.0593), there was a highlevel of gene flow in the Ascaris populations. A hierarchical analysis on molecular variance revealed remarkably high levels of variation within the populations. Moreover, a population structure analysis indicated that Ascaris populations fell into 3 main genetic clusters, interpreted as A. suum, A. lumbricoides, and a hybrid of the species. We speculated that humans can be infected with A. lumbricoides, A. suum, and the hybrid, but pigs were mainly infected with A. suum. This study provided new information on the genetic diversity and population structure of Ascaris from human and pigs in China, which can be used for designing Ascaris control strategies. It can also be beneficial to understand the introgression of host affiliation.

Citations

Citations to this article as recorded by  Crossref logo
  • Development and cross-amplification of novel SSR markers for population genetic analysis of Kitti’s hog-nosed bat (Craseonycteris thonglongyai) in Thailand
    Supajit Sraphet, Nattaya Srisawad, Nawarat Suksee, Piengtawan Tappiban, Surachit Waengsothorn, Kridsada Chaichoun, Ruangrat Buddhirongawatr, Sarin Suwanpakdee, Siriporn Tungsudjai, Poonyapat Sedwisai, Tatiyanuch Chamsai, Thekhawet Weluwanarak, Nareerat Sa
    Scientific Reports.2025;[Epub]     CrossRef
  • Worldwide absence of canonical benzimidazole resistance-associated mutations within β-tubulin genes from Ascaris
    Ben P. Jones, Kezia Kozel, Allen Jethro I. Alonte, Kennesa Klariz R. Llanes, Alexandra Juhász, Umer Chaudhry, Sara Roose, Peter Geldhof, Vicente Y. Belizario, Peter Nejsum, J. Russell Stothard, E. James LaCourse, Arnoud H. M. van Vliet, Vachel Gay V. Pall
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Ascaris suum – A zoonosis in Bali, Indonesia
    Kadek Karang Agustina, I Made Ady Wirawan, I Made Sudarmaja, I Made Subrata, Nyoman Sadra Dharmawan
    Tropical Parasitology.2023; 13(2): 100.     CrossRef
  • Molecular epidemiology ofAscarisspecies recovered from humans and pigs in Cameroon
    Vanessa R Nkouayep, Donald P McManus, Mpoame Mbida, Catherine A Gordon, Peter Nejsum
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2022; 116(10): 949.     CrossRef
  • Development of allele-specific PCR methodology (AS-PCR) to screening A. lumbricoides and A. suum
    Talita Rodrigues dos Santos, Luis Fernando Viana Furtado, Andreina de Carvalho Araujo, Celi da Silva Medeiros, Pedro Henrique Vieira Germano, Valéria Nayara Gomes Mendes de Oliveira, Elida Mara Leite Rabelo
    Parasitology Research.2022; 121(8): 2389.     CrossRef
  • Genotyping of Ascaris spp. infecting humans and pigs in Italy, Slovakia and Colombia
    Serena Cavallero, Silvia Rondón, Ivan Acevedo Monterrosa, Viliam Šnábel, Ingrid Papajová, Mária Goldová, Gabriela Štrkolcová, Luis Caraballo, Nathalie Acevedo, Stefano D'Amelio
    Infection, Genetics and Evolution.2021; 94: 104997.     CrossRef
  • Mitochondrial phylogenomics of human-type Ascaris, pig-type Ascaris, and hybrid Ascaris populations
    Chunhua Zhou, Ting Guo, Yuanyu Deng, Jingjing He, Shan Ouyang, Xiaoping Wu
    Veterinary Parasitology.2020; 287: 109256.     CrossRef
  • 8,339 View
  • 128 Download
  • 5 Web of Science
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Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea
Tong-Il Choi, Eui-Ju Hong, Si-Yun Ryu, Cheolho Sim, Joon-Seok Chae, Hyeon-Cheol Kim, Jinho Park, Kyoung-Seong Choi, Do-Hyeon Yu, Jae-Gyu Yoo, Bae-Keun Park
Korean J Parasitol 2018;56(2):121-127.
Published online April 30, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.2.121
To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21-1.25 μm in length and 0.05-0.07 μm in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Sarcocystis species: molecular identification and seroprevalence in water buffaloes (Bubalus bubalis)
    Nagwa I. Toaleb, Raafat M. Shaapan, Haitham Elaadli, Kadria N. Abdel Megeed, Dina Aboelsoued
    BMC Veterinary Research.2025;[Epub]     CrossRef
  • Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt
    Lamiaa K. Elsharkawy, Safaa M. Barghash, Basma M. Abou El-Nour, Wafaa Labib, Al-Shaimaa M. Sadek
    BMC Veterinary Research.2024;[Epub]     CrossRef
  • Molecular Identification, Pathogenesis, and Life Cycle of Sarcocystis cruzi from Cattle (Bos taurus) in New Valley Governorate, Egypt
    Mohammed B. M. El-Mahdi, Soheir A. Rabie, Reda M. El-S. Hassanine, Amal A. Hassan, Obaida F. Abo Elhussien, Mamdooh Ghoneum, Mohamed S. A. El-Gerbed, Eric Agola Lelo
    Journal of Parasitology Research.2023; 2023: 1.     CrossRef
  • Molecular Epidemiology, Species Distribution, and Zoonotic Importance of the Neglected Meat-Borne Pathogen Sarcocystis spp. in Cattle (Bos taurus): A Global Systematic Review and Meta-analysis
    Morteza Shams, Laya Shamsi, Ali Asghari, Mohammad Hossein Motazedian, Behnam Mohammadi-Ghalehbin, Mostafa Omidian, Naser Nazari, Alireza Sadrebazzaz
    Acta Parasitologica.2022; 67(3): 1055.     CrossRef
  • Infection survey and morphological characteristics of Sarcocystis spp. in naturally infected Tibetan sheep from Qinghai in northwestern China
    Yali Sun, Jinling Ju, Xiaoxue Su, Caiying Xie, Ying Li, Ming Kang
    Parasitology International.2021; 80: 102219.     CrossRef
  • Meat-borne parasites in the Arab world: a review in a One Health perspective
    Sameh Abuseir
    Parasitology Research.2021; 120(12): 4153.     CrossRef
  • Opportunistic parasitic infections in patients with human immunodeficiency virus/acquired immunodeficiency syndrome: A review
    D. A. Laksemi, L. T. Suwanti, M. Mufasirin, K. Suastika, M. Sudarmaja
    Veterinary World.2020; 13(4): 716.     CrossRef
  • Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea
    Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park
    The Korean Journal of Parasitology.2020; 58(4): 403.     CrossRef
  • Prevalence and molecular analysis of Sarcocystis infections in cattle in Northwest Iran and the first global report of S. gigantea in cattle
    Nazila Sarafraz, Adel Spotin, Ali Haniloo, Asghar Fazaeli
    Comparative Immunology, Microbiology and Infectious Diseases.2020; 73: 101566.     CrossRef
  • Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method
    Petras Prakas, Živilė Strazdaitė-Žielienė, Vytautas Januškevičius, Francesco Chiesa, Agnė Baranauskaitė, Eglė Rudaitytė-Lukošienė, Elena Servienė, Saulius Petkevičius, Dalius Butkauskas
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Ultrastructural and Molecular Identification of the sarcocysts of Sarcocystis tenella and Sarcocystis arieticanis Infecting Domestic Sheep (Ovis aries) from Egypt
    Ahmed El-Morsey, Walied Abdo, Khaled Sultan, Nagwa M. Elhawary, Attia Abdallah AbouZaid
    Acta Parasitologica.2019; 64(3): 501.     CrossRef
  • Sarcocystis spp. in Romanian Slaughtered Cattle: Molecular Characterization and Epidemiological Significance of the Findings
    Kálmán Imre, Gheorghe Dărăbuș, Emil Tîrziu, Sorin Morariu, Mirela Imre, Judit Plutzer, Marius V. Boldea, Adriana Morar
    BioMed Research International.2019; 2019: 1.     CrossRef
  • 9,635 View
  • 143 Download
  • 13 Web of Science
  • Crossref

Brief Communication

Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein
Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Ronaldo F. Dias, Ho-Woo Nam
Korean J Parasitol 2016;54(2):239-241.
Published online April 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.2.239
Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection.

Citations

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  • Medical viruses: diagnostic techniques
    Harsh, Pratima Tripathi
    Virology Journal.2023;[Epub]     CrossRef
  • Diagnostic accuracy of serological tests for the diagnosis of Chikungunya virus infection: A systematic review and meta-analysis
    Anna Andrew, Tholasi Nadhan Navien, Tzi Shien Yeoh, Marimuthu Citartan, Ernest Mangantig, Magdline S. H. Sum, Ewe Seng Ch’ng, Thean-Hock Tang, Guilherme S. Ribeiro
    PLOS Neglected Tropical Diseases.2022; 16(2): e0010152.     CrossRef
  • An Overview of Laboratory Diagnosis of Central Nervous System Viral Infections
    Sharifah Aliah Diyanah Syed Hussin, Ang-Lim Chua, Hassanain Al-Talib, Shamala Devi Sekaran, Seok Mui Wang
    Journal of Pure and Applied Microbiology.2022; 16(4): 2225.     CrossRef
  • Molecular and Immunological Diagnostic Techniques of Medical Viruses
    Daniel Hussien Reta, Tesfaye Sisay Tessema, Addis Simachew Ashenef, Adey Feleke Desta, Wajana Lako Labisso, Solomon Tebeje Gizaw, Solomon Mequanente Abay, Daniel Seifu Melka, Fisseha Alemu Reta
    International Journal of Microbiology.2020; 2020: 1.     CrossRef
  • Apigenin enhances the cisplatin cytotoxic effect through p53-modulated apoptosis
    Rui Liu, Ping Ji, Bin Liu, Haishi Qiao, Xia Wang, Likun Zhou, Ting Deng, Yi Ba
    Oncology Letters.2017; 13(2): 1024.     CrossRef
  • 9,202 View
  • 164 Download
  • 6 Web of Science
  • Crossref

Original Articles

Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella
Bian-hua Zhou, Li-li Liu, Jeffrey Liu, Fu-wei Yuan, Er-jie Tian, Hong-wei Wang
Korean J Parasitol 2015;53(6):675-682.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.675
The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.

Citations

Citations to this article as recorded by  Crossref logo
  • Experimental Eimeria tenella infection of chickens followed by very virulent infectious bursal disease viral challenge: clinical and pathological effects
    Nahed A. El-Shall, Mahmoud E. Sedeik, Heba M. Ismail, Ashraf M. Awad
    Avian Pathology.2025; : 1.     CrossRef
  • Identification of differentially expressed genes and metabolism signaling pathway in the spleen of broilers supplemented with probiotic Bacillus spp.
    Anh Duc Truong, Ha Thi Thanh Tran, Huyen Thi Nguyen, Nhu Thi Chu, Lanh Phan, Hoai Thi Phan, Ngoc Thi Pham, Van Hai Nguyen, Lan Huong Nguyen, Dang Kim Pham, Phu- Ha Ho, Hoang Vu Dang
    Veterinary Immunology and Immunopathology.2024; 272: 110755.     CrossRef
  • Efficacy and safety of diclazuril nanoemulsion in control of Eimeria tenella in broilers
    Azza A. El-Sawah, Shawky M. Aboelhadid, El-Shymaa N. El-Nahass, Hassan E. Helal
    BMC Veterinary Research.2024;[Epub]     CrossRef
  • Diclazuril-induced expression of CDK-related kinase 2 in the second-generation merozoites of Eimeria tenella
    Bian-hua Zhou, Hai-yan Ding, Jing-yun Yang, Jun Chai, Hong-wei Guo, Er-jie Tian
    Molecular and Biochemical Parasitology.2023; 255: 111575.     CrossRef
  • Ameliorative effect of yeast cell walls on broiler chickens' performance and gut health under coccidiosis challenge
    Shawkat Abdulrazaq M'Sadeq
    Czech Journal of Animal Science.2023; 68(8): 346.     CrossRef
  • In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens
    Hyun-Gwan Lee, Yoo-Bhin Kim, Sang-Hyeok Lee, Jun-Ok Moon, Jong-Pyo Chae, Yu-Jin Kim, Kyung-Woo Lee
    Veterinary Sciences.2022; 9(3): 119.     CrossRef
  • EFFECTS OF ZN2+- AND CR3+-MODIFIED PALYGORSKITE ON THE TREATMENT OF EIMERIA TENELLA
    D.W. Yao, F.M. Khand, Y Xu, Q.Q. Shen, Y Wang, D.J. Yang
    The Journal of Animal and Plant Sciences.2022; 32(2): 397.     CrossRef
  • Prolonging and enhancing the protective efficacy of the EtMIC3-C-MAR against eimeria tenella through delivered by attenuated salmonella typhimurium
    Ningning Zhao, Junfeng Lv, Yaru Lu, Yingying Jiang, Hongmei Li, Yumin Liu, Xiao Zhang, Xiaomin Zhao
    Veterinary Parasitology.2020; 279: 109061.     CrossRef
  • Efficacy of probiotic Enterococcus faecium in combination with diclazuril against coccidiosis in experimentally infected broilers
    A. A. El‐Sawah, S. M. Aboelhadid, E. N. El‐Nahass, H. E. Helal, A. M. Korany, S. El‐Ashram
    Journal of Applied Microbiology.2020; 129(4): 1020.     CrossRef
  • Dual-sensitive chitosan derivative micelles for site-specific drug release in the treatment of chicken coccidiosis
    Xin Zhang, Gujun Xu, Khalid Gadora, Hao Cheng, Jin Peng, Yong Ma, Yang Guo, Cheng Chi, Jianping Zhou, Yang Ding
    RSC Advances.2018; 8(26): 14515.     CrossRef
  • Combination of purine and pyrimidine nucleosides influences growth performance, gut morphology, digestive enzymes, serum biochemical indices and immune functions in broiler chickens
    Ali Daneshmand, Hassan Kermanshahi, Mohsen Danesh Mesgaran, Annie J. King, Salam A. Ibrahim, Kirk C. Klasing
    Animal Feed Science and Technology.2017; 228: 186.     CrossRef
  • Analysis of Differentially Expressed Genes in Necrotic Enteritis-infected Fayoumi Chickens using RNA Sequencing
    Anh Duc Truong, Yeojin Hong, Jihye Ban, Boyeong Park, Thanh C. Hoang, Yeong H. Hong, Hyun S. Lillehoj
    The Journal of Poultry Science.2017; 54(2): 121.     CrossRef
  • Effect of purine nucleosides on growth performance, gut morphology, digestive enzymes, serum profile and immune response in broiler chickens
    A. Daneshmand, Hassan Kermanshahi, M. Danesh Mesgaran, A. J. King, S. A. Ibrahim
    British Poultry Science.2017; 58(5): 536.     CrossRef
  • Effects of pyrimidine nucleosides on growth performance, gut morphology, digestive enzymes, serum biochemical indices and immune response in broiler chickens
    A. Daneshmand, H. Kermanshahi, M. Danesh Mesgaran, A.J. King, S.A. Ibrahim
    Livestock Science.2017; 204: 1.     CrossRef
  • 14,927 View
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  • 14 Web of Science
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High Expression of Water-Soluble Recombinant Antigenic Domains of Toxoplasma gondii Secretory Organelles
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2014;52(4):367-376.
Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.367

Recombinant antigenic proteins of Toxoplasma gondii are alternative source of antigens which are easily obtainable for serodiagnosis of toxoplasmosis. In this study, highly antigenic secretory organellar proteins, dense granular GRA2 and GRA3, rhoptrial ROP2, and micronemal MIC2, were analyzed by bioinformatics approach to express as water-soluble forms of antigenic domains. The transmembrane region and disorder tendency of 4 secretory proteins were predicted to clone the genes into pGEX-4T-1 vector. Recombinant plasmids were transformed into BL21 (DE3) pLysS E. coli, and GST fusion proteins were expressed with IPTG. As a result, GST fusion proteins with GRA225-105, GRA339-138, ROP2324-561, and MIC21-284 domains had respectively higher value of IgG avidity. The rGST-GRA225-105 and rGST-GRA339-138 were soluble, while rGST-ROP2324-561 and rGST-MIC21-284 were not. GRA231-71, intrinsically unstructured domain (IUD) of GRA2, was used as a linker to enhance the solubility. The rGST-GRA231-71-ROP2324-561, a chimeric protein, appeared to be soluble. Moreover, rGST-GRA231-71-MIC21-284 was also soluble and had higher IgG avidity comparing to rGST-MIC21-284. These 4 highly expressed and water-soluble recombinant antigenic proteins may be promising candidates to improve the serodiagnosis of toxoplasmosis in addition to the major surface antigen of SAG1.

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  • Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Microorganisms.2024; 12(8): 1731.     CrossRef
  • A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
    Macarena Sarli, María B. Novoa, Matilde N. Mazzucco, Marcelo L. Signorini, Ignacio E. Echaide, Susana T. de Echaide, María E. Primo, Paulo Lee Ho
    PLOS ONE.2020; 15(2): e0229301.     CrossRef
  • Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
    María E. Primo, Carolina S. Thompson, Beatriz S. Valentini, Macarena Sarli, María B. Novoa, Atilio J. Mangold, Susana T. de Echaide, Ulrike Gertrud Munderloh
    PLOS ONE.2019; 14(1): e0211149.     CrossRef
  • TheToxoplasma gondiidense granule protein TgGRA3 interacts with host Golgi and dysregulates anterograde transport
    Maika S. Deffieu, Tchilabalo Dilezitoko Alayi, Christian Slomianny, Stanislas Tomavo
    Biology Open.2019;[Epub]     CrossRef
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Curcumin Prevents Bile Canalicular Alterations in the Liver of Hamsters Infected with Opisthorchis viverrini
Prapaporn Jattujan, Somchai Pinlaor, Lakhanawan Charoensuk, Channarong Arunyanart, Jariya Umka Welbat, Wunnee Chaijaroonkhanarak
Korean J Parasitol 2013;51(6):695-701.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.695

Opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. Little is known about the pathological alterations in bile canaliculi in opisthorchiasis. This study aimed to investigate bile canalicular alterations in O. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. Hamsters were infected with O. viverrini and one group of animals was fed with 1% dietary curcumin supplement. Animals were examined during the acute infection phase, days 21 and 30 post-infection (PI) and chronic infection phase (day 90 PI). Scanning electron microscopy revealed that in the infected group fed with a normal diet, bile canaliculi became slightly tortuous by 30 day PI and more tortuous at day 90 PI. Transmission electron microscopy showed a reduction in microvilli density of canaliculi starting at day 30 PI, with a marked loss of microvilli at day 90 PI. These ultrastructral changes were slightly seen at day 21 PI, which was similar to that found in infected animals fed with 1% curcumin-supplemented diet. Notably, curcumin treatment prevented the reduction of microvilli density, reduced the dilation of bile canaliculi, and decreased the tortuosity of the bile canaliculi relative to non-infected animals on a normal diet at days 30 and 90 PI. These results suggest that curcumin reduces alteration of bile canaliculi and may be a promising agent to prevent the onset of bile duct abnormalities induced by O. viverrini infection.

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  • Components from Curcuma longa (Turmeric) Against Hepatobiliary Diseases Based on Gut–Liver Axis: Pharmacotherapeutic Properties and Potential Clinical Applications
    Tianhui Gao, Shuyi Wang, Zongping Zhu, Liting Lin, Yirong Luo, Meigui Lu, Wan Liao
    The American Journal of Chinese Medicine.2024; 52(02): 387.     CrossRef
  • Dietary Polyphenols Alleviate Autoimmune Liver Disease by Mediating the Intestinal Microenvironment: Challenges and Hopes
    Zhen Cheng, Yuehua Wang, Bin Li
    Journal of Agricultural and Food Chemistry.2022; 70(35): 10708.     CrossRef
  • Generation of oxidative stress and induction of apoptotic like events in curcumin and thymoquinone treated adult Fasciola gigantica worms
    Abdur Rehman, Rizwan Ullah, Divya Gupta, M.A. Hannan Khan, Lubna Rehman, Mirza Ahmar Beg, Asad U. Khan, S.M.A. Abidi
    Experimental Parasitology.2020; 209: 107810.     CrossRef
  • Nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in Opisthorchis viverrini-infected hamsters
    Lakhanawan Charoensuk, Porntip Pinlaor, Supason Wanichwecharungruang, Kitti Intuyod, Kulthida Vaeteewoottacharn, Apisit Chaidee, Puangrat Yongvanit, Chawalit Pairojkul, Natthakitta Suwannateep, Somchai Pinlaor
    Nanomedicine: Nanotechnology, Biology and Medicine.2016; 12(1): 21.     CrossRef
  • Bile canalicular changes and defective bile secretion in Opisthorchis viverrini-infected hamsters
    Lakhanawan Charoensuk, Porntip Pinlaor, Umawadee Laothong, Puangrat Yongvanit, Chawalit Pairojkul, Yukifumi Nawa, Somchai Pinlaor
    Folia Parasitologica.2014; 61(6): 512.     CrossRef
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Ultrastructure of Spermatogenesis in the Testis of Paragonimus heterotremus
Nongnut Uabundit, Pipatphong Kanla, Phongphithak Puthiwat, Channarong Arunyanart, Kowit Chaiciwamongkol, Wanchai Maleewong, Pewpan M. Intapan, Sitthichai Iamsaard, Wiphawi Hipkaeo
Korean J Parasitol 2013;51(6):669-676.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.669

Lung fluke, Paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in Southeast Asia. We examined the ultrastructure of the testis of adult P. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. The full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. The sequence comprises spermatogonia, spermatocytes with obvious nuclear synaptonemal complexes, spermatids, and eventual spermatozoa. Moreover, full steps of spermatid differentiation were shown which consisted of 1) early stage, 2) differentiation stage representing the flagella, intercentriolar body, basal body, striated rootlets, and electron dense nucleus of thread-like lamellar configuration, and 3) growing spermatid flagella. Detailed ultrastructure of 2 different types of spermatozoa was also shown in this study.

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  • Molecular identification of the trematode P. ichunensis stat. n. from lungs of siberian tigers justified reappraisal of Paragonimus westermani species complex
    Anastasia N. Voronova, Konstantin S. Vainutis, Tatiana V. Tabakaeva, Mikhail V. Sapotsky, Nadezhda N. Kakareka, Yury G. Volkov, Irina V. Galkina, Mikhail Yu. Shchelkanov
    Journal of Parasitic Diseases.2022; 46(3): 744.     CrossRef
  • 10,768 View
  • 116 Download
  • Crossref
A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
Korean J Parasitol 2013;51(5):503-510.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.503

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.

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  • Development of an Immunochromatographic Test with Recombinant MIC2-MIC3 Fusion Protein for Serological Detection of Toxoplasma gondii
    Jianzhong Wang, Yi Zhao, Jicheng Qiu, Jing Liu, Rui Zhou, Xialin Ma, Xiaojie Wu, Xiaoguang Li, Wei Mao, Yiduo Liu, Heng Zhang
    Veterinary Sciences.2025; 12(6): 509.     CrossRef
  • Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species
    Jiyuan Fan, Hao Sun, Jiawen Fang, Yafan Gao, Haojie Ding, Bin Zheng, Qingming Kong, Xunhui Zhuo, Shaohong Lu
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis
    Karolina Sołowińska, Lucyna Holec-Gąsior
    Microorganisms.2024; 12(8): 1731.     CrossRef
  • Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China
    Yu-bin Guan, Xiao-xiao Sun, Shao-lian Chen, Xiao-ting Zhu, Zhi-hua Zeng, Han-wei Lu, Hong-mei Feng, Yu Guo, Wen-gong Jiang, Kui Xiong, Xiao-rong Yang, Ho-Woo Nam, Zhao-shou Yang, Pan Li
    PLOS ONE.2023; 18(7): e0286430.     CrossRef
  • Past and present seroprevalence and disease burden estimates of Toxoplasma gondii infections in Germany: An appreciation of the role of serodiagnostics
    Frank Seeber
    International Journal of Medical Microbiology.2023; 313(6): 151592.     CrossRef
  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
    Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(3): 251.     CrossRef
  • Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals
    Amjad Hayat Khan, Rahmah Noordin
    European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 19.     CrossRef
  • Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
    Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2020; 58(5): 493.     CrossRef
  • Identification of novel antigens for serum IgG diagnosis of human toxoplasmosis
    Juntao Luo, Jingyi Wan, Ziru Tang, Shuang Shen
    Experimental Parasitology.2019; 204: 107722.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample
    Lívia M. Alves, Vinícius R. Rodovalho, Ana C.H. Castro, Márcia A.R. Freitas, Caroline M. Mota, Tiago W.P. Mineo, José R. Mineo, João M. Madurro, Ana G. Brito-Madurro
    Journal of Pharmaceutical and Biomedical Analysis.2017; 145: 838.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef
  • Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein
    Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Ronaldo F. Dias, Ho-Woo Nam
    The Korean Journal of Parasitology.2016; 54(2): 239.     CrossRef
  • Digested disorder, Quarterly intrinsic disorder digest (October-November-December, 2013)
    Shelly DeForte, Krishna D Reddy, Vladimir N Uversky
    Intrinsically Disordered Proteins.2015; 3(1): e984569.     CrossRef
  • Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients
    Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim
    The Korean Journal of Parasitology.2014; 52(2): 143.     CrossRef
  • High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles
    Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2014; 52(4): 367.     CrossRef
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Brief Communication

Cercaria caribbea LVIII Cable, 1963 (Digenea: Cyathocotylidae) in the Republic of Korea and Its Surface Ultrastructure
Eun-Taek Han, Jeong-Hyun Park, Jong-Yil Chai
Korean J Parasitol 2012;50(2):177-180.
Published online May 24, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.2.177

Cercaria caribbea LVIII Cable, 1963 (Digenea: Cyathocotylidae) was detected from a brackish water gastropod species (Cerithideopsilla cingulata) in a coatal area of Shinan-gun, Jeollanam-do (Province), the Republic of Korea, and its surface ultrastructure was studied using a scanning electron microscope. The cercariae were found freely swimming or enveloped within daughter sporocysts when the snail host was mechanically broken. They were morphologically characterized by a linguiform and ventrally concave body, a long and bifurcated tail, and the presence of a holdfast (=tribocytic) organ posterior to the ventral sucker. On the whole ventral and dorsal surfaces, peg-like tegumental spines were densely distributed. Around the oral sucker, several sensory papillae, each with a short cilium, were distributed, and on the tail, sensory papillae, each with an extensively long cilium, were observed. This is the first record describing a cyathocotylid cercaria from a brackish water gastropod in the Republic of Korea.

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  • A preliminary study on some larval trematodes parasites of marine snail Cerithidea cingulata (Gmelin, 1791) in Al- faw Bay, South of Iraq

    International Journal of Biosciences (IJB).2020; : 464.     CrossRef
  • 7,979 View
  • 87 Download
  • Crossref

Original Article

Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation
Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu
Korean J Parasitol 2011;49(1):25-31.
Published online March 18, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.1.25

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.

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  • Real-time detection of 3–8-µm microbial particles in sand filter effluent as a surrogate indicator of Cryptosporidium parvum oocysts
    Sandrine Boivin, Hiroshi Nagaoka, Takahiro Fujioka
    Water Research X.2025; 27: 100345.     CrossRef
  • Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii
    Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert
    Parasitology Research.2023; 122(8): 1819.     CrossRef
  • Discovery of New Microneme Proteins in Cryptosporidium parvum and Implication of the Roles of a Rhomboid Membrane Protein (CpROM1) in Host–Parasite Interaction
    Xin Gao, Jigang Yin, Dongqiang Wang, Xiaohui Li, Ying Zhang, Chenchen Wang, Yuanyuan Zhang, Guan Zhu
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • A new method for efficient detection of Cryptosporidium RNA by real-time reverse transcription-PCR with surfactants
    Takahiro Sekikawa, Kosuke Toshiki
    Water Supply.2015; 15(5): 1061.     CrossRef
  • 8,351 View
  • 92 Download
  • Crossref

Brief Communication

Ultrastructural Localization of Toxocara canis Larval Antigen Reacted with a Seropositive Human Serum
Soo-Ung Lee, Jae-Ran Yu, Sun Huh
Korean J Parasitol 2009;47(1):65-68.
Published online March 12, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.1.65

Excretory-secretory products of Toxocara canis larvae have been considered as a major functional antigen in immune responses against toxocariasis. We studied ultrastructural localization of T. canis second-stage larval antigen using a seropositive human serum under immunogold electron microscopy. High-density gold particles were observed in the secretory cells, excretory duct, intestinal epithelium, and cuticle of the larval worm sections. The distribution of the positive reactions in the larval worms suggests that the nature of the antigen is excretory-secretory antigen including waste metabolites and secretory enzymes.

Citations

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  • Toxocara canis-originated recombinant C-type lectin improves the disability scores of experimental autoimmune encephalomyelitis in murine in vivo models
    Mahsa Shahbakhsh, Fateme Jalousian, Seyed Hossein Hosseini, Abdorreza Naser Moghadasi, Parviz Shayan, Samad Farashi Bonab, Parmida Malekzade, Mohammad Vojgani, Mahya Lalehpour
    Journal of Neuroimmunology.2025; 402: 578569.     CrossRef
  • Producción y evaluación del antígeno recombinante Tes-30 de Toxocara canis para el inmunodiagnóstico de toxocariasis
    Ana M. Olave, Jairo A. Mesa, Jorge H. Botero, Edwin B. Patiño, Gisela M. García, Juan F. Alzate
    Biomédica.2015;[Epub]     CrossRef
  • Evaluación de un antígeno purificado para el diagnóstico de toxocariosis
    Graciela Santillán, Vanesa Bastin, Graciela Céspedes, Adriana Monkiewicz
    Revista Argentina de Microbiología.2013; 45(2): 80.     CrossRef
  • Frequency of unexpected antibody and consideration during transfusion
    Ki-Ho Ko, Byung-Hoon Yoo, Kye-Min Kim, Woo-Yong Lee, Jun-Heum Yon, Ki-Hyuk Hong, Tae-Hee Han
    Korean Journal of Anesthesiology.2012; 62(5): 412.     CrossRef
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Original Articles

Tegumental Ultrastructure of Adult Gynaecotyla squatarolae (Digenea: Microphallidae)
Do-Seon Lim, Ki-Ju Choi, Sang-Mee Guk, Jong-Yil Chai, Il-Yong Park, Yun-Kyu Park, Min Seo
Korean J Parasitol 2008;46(2):87-90.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.87

Gynaecotyla squatarolae (Digenea: Microphallidae) adult flukes were recovered from experimental chicks at day 4-6 post-infection and their tegumental ultrastructure was observed with a scanning electron microscopy. They were pyriform in shape, and their anterior halves were concaved ventrally. The whole body surface was covered with tegumental spines, which were wide and 16-17 digitated between oral and ventral suckers. The density of spines and number of digits decreased posteriorly. The oral sucker was subterminal and the excretory pore was at the posterior end of the worm. Two ventral suckers were similar in appearance and protruded near midline of the worm. The genital atrium was dextral to the small ventral sucker. The dorsal surface was covered with tegumental spines, but the spines were sparser than on the ventral surface. On the middle portion of the dorsal surface, a small opening presumed to be the Laurer's canal was seen. From these findings, it has been confirmed that the adult G. squatarolae has unique characteristics in the surface ultrastructure.

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  • Two new species of Parspina Pearse, 1920 (Digenea: Cryptogonimidae) from freshwater fishes (Gymnotiformes) of the Paraná River basin in Argentina
    Margarita C. Ostrowski de Núñez, Nathalia J. Arredondo, Alicia A. Gil de Pertierra
    Systematic Parasitology.2011; 80(1): 67.     CrossRef
  • Ultrastructural features of the tegumental surface of a new metacercaria, Nematostrigea sp. (Trematoda: Strigeidae), with a search for potential taxonomically informative characters
    Larisa G. Poddubnaya, Elena Mishina, Alexander E. Zhokhov, David I. Gibson
    Systematic Parasitology.2010; 75(1): 59.     CrossRef
  • 7,925 View
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Developmental surface ultrastructure of Macroorchis spinulosus in albino rats
Sung-Jong Hong, Ho-Chun Woo, Oh Sil Kwon
Korean J Parasitol 2004;42(4):151-157.
Published online December 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.4.151

Developmental surface ultrastructure of Macroorchis spinulosus was studied by scanning electron microscopy. One-day-old juvenile fluke was leaf-shaped and bent ventrally. Body surface was covered densely with peg-like spines and with cobblestone-like cytoplasmic processes. Ciliated sensory papillae were concentrated around oral sucker. Several unciliated sensory papillae occurred equidistantly on oral sucker and acetabulum. The ciliated papillae appeared in two longitudinal lines symmetric bilaterally on dorsal surface. On adult flukes, tegumantal spines became wider in middle of the body surface. The cytoplasmic processes differentiated into more fine velvety form. It is likely that the differentiated fine cytoplasmic processes are an increased absorptive surface to adult M. spinulosus. It is suggested that single pointed tegumental spines on anterior half of the body may be supportive for this fluke to migration.

Citations

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  • Evaluation of the effect of larval metamorphosis of some digenetic parasites within the digestive gland tissues of the freshwater ampullariid snails, Lanistes carinatus (Olivier, 1804)
    Mohammed E. El-Zeiny, Ola A. Abu Samak, Shereen A. Fahmy, Abdel Aziz A. Khidr
    Parasitology International.2025; 108: 103078.     CrossRef
  • Lethal effects of praziquantel and albendazole, on the cercariae of Echinochasmus sp. (Dietz, 1909) in-vitro
    Mohammed E. El-Zeiny, Ola A. Abu Samak, Shereen A. Fahmy, Abdel Aziz A. Khidr
    Journal of Parasitic Diseases.2024; 48(2): 320.     CrossRef
  • Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences
    Eun Jeong Won, Deok-Gyu Kim, Jaeeun Cho, Bong-Kwang Jung, Min-Jae Kim, Yong Woon Yun, Jong-Yil Chai, Dong Wook Ryang
    The Korean Journal of Parasitology.2016; 54(1): 109.     CrossRef
  • Surface ultrastructure of Plagiorchis muris growth and developmental stages in rats, the final host
    Sung-Jong Hong
    Parasitology Research.2009; 105(4): 1077.     CrossRef
  • Surface ultrastructure of the plagiorchid trematode Glossidium pedatum Looss, 1899 from bagrid fish in Egypt
    Mohammed Hasan Ibraheem
    Acta Zoologica.2007; 88(2): 173.     CrossRef
  • MORPHOLOGICAL OBSERVATIONS OF ECHINOCHASMUS JAPONICUS CERCARIAE AND THE IN VITRO MAINTENANCE OF ITS LIFE CYCLE FROM CERCARIAE TO ADULTS
    Min-Ho Choi, Sae Hoon Kim, Jong-Hoon Chung, Hye-Jin Jang, Joon-Ho Eom, Byung-Suk Chung, Woon-Mok Sohn, Jong-Yil Chai, Sung-Tae Hong
    Journal of Parasitology.2006; 92(2): 236.     CrossRef
  • 7,269 View
  • 65 Download
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Brief Communication

Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae)
Woon-Mok Sohn, Min Seo, Jong-Yil Chai
Korean J Parasitol 2003;41(2):107-111.
Published online June 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.2.107

The surface ultrastructure of Acanthotrema felis (Trematoda: Heterophyidae) adults, recovered from a kitten experimentally infected with the metacercariae, was observed using a scanning electron microscope. The worm was leaf-like, ventrally concave and covered with scale-like multi-pointed tegumental spines. The spines on the anterior surface were short but broad, and had 10-12 pointed tips. The cytoplasmic processes protruded around the spines, like pockets for the spines. The ventrogenital opening was crescent, or kidney-shaped, and had protuberances with minute spines on its surrounding tegument. The spines on the posterior surface were long, but narrow, with 6-8 pointed tips. The cytoplasmic processes on this tegument were ridge-like, and elevated along the row of the spines. The surface ultrastructure of A. felis is generally similar to that of other heterophyid flukes, but some features are characteristic, and may be of taxonomic and bio-ecological significance.

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  • Body Surface Ultrastructure as a Main Morphological Criterion for Distinguishing Adult Trematode Metagonimus suifunensis
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  • Surface ultrastructure of the adult and juvenile stages of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 (incertae sedis) from the Nile puffer, Tetraodon lineatus Linnaeus, 1758
    S. G. Abd El-Kareem, M. H. Ibraheem
    Helminthologia.2021; 58(2): 188.     CrossRef
  • REDESCRIPTION AND SURFACE ULTRASTRUCTURE OF PYGIDIOPSIS MACROSTOMUM (DIGENEA: HETEROPHYIDAE)
    S. B E. Simões, H. S. Barbosa, C. P. Santos
    Journal of Parasitology.2005; 91(4): 931.     CrossRef
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Original Articles

Tegumental ultrastructure of the juvenile and adult Himasthla alincia (Digenea: Echinostomatidae)
Eun-Taek Han, Kye-Young Han, Jong-Yil Chai
Korean J Parasitol 2003;41(1):17-25.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.17

The tegumental ultrastructure of juvenile and adult Himasthla alincia (Digenea: Echinostomatidae) was observed by scanning electron microscopy. One-, 5- (juveniles) and 20-day-old worms (adults) were harvested from chicks experimentally fed metacercariae from a bivalve, Mactra veneriformis. The juvenile worms were elongated and curved ventrally. The head crown bore 31 collar spines, arranged in a single row. The lip of the oral sucker had 12 paired, and 3 single type I sensory papillae, and the ventral sucker had about 25 type II sensory papillae. The anterolateral surface between the two suckers was densely packed with tegumental spines with 4-7 pointed tips. The adult worms were more elongated and filamentous, and had severe transverse folds over the whole body surface. On the head crown and two suckers, type I and II sensory papillae were more densely distributed than in the juvenile worms. Retractile brush-like spines, with 8-10 digits, were seen on the anterolateral surface, whereas claw-shaped spines, with 2-5 digits, were sparsely distributed posteriorly to the ventral sucker. The cirrus characteristically protruded out, and was armed with small spines distally. The surface ultrastructure of H. alincia was shown to be unique among echinostomes, especially in the digitation of its tegumental spines, the distribution of sensory papillae and by severe folds of the tegument.

Citations

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  • Surface ultrastructure of the adult and juvenile stages of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 (incertae sedis) from the Nile puffer, Tetraodon lineatus Linnaeus, 1758
    S. G. Abd El-Kareem, M. H. Ibraheem
    Helminthologia.2021; 58(2): 188.     CrossRef
  • Intestinal Flukes Recovered from a Herring Gull, Larus argentatus, in the Republic of Korea
    Young-Il Lee, Min Seo, Jong-Yil Chai
    The Korean Journal of Parasitology.2020; 58(1): 81.     CrossRef
  • Tegumental ultrastructure of adult Quinqueserialis quinqueserialis (Trematoda: Notocotylidae): an intestinal parasite of muskrat (Ondatra zibethicus)
    Soraya Naem, Ashleigh B. Smythe
    Parasitology Research.2015; 114(7): 2473.     CrossRef
  • Three Echinostome Species from Wild Birds in the Republic of Korea
    Seongjun Choe, Dongmin Lee, Hansol Park, Mihyeon Oh, Hyeong-Kyu Jeon, Youngsun Lee, Ki-Jeong Na, Youngjun Kim, Hang Lee, Keeseon S. Eom
    The Korean Journal of Parasitology.2014; 52(5): 513.     CrossRef
  • Topography and ultrastructure of the tegument of Aphallus tubarium (Rodolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), intestinal parasite of the common Dentex dentex (Linnaeus 1758) from Valinco Gulf
    Laetitia Antonelli, Yann Quilichini, Jos?phine Foata, Bernard Marchand
    Acta Parasitologica.2014;[Epub]     CrossRef
  • Echinostoma macrorchis in Lao PDR: Metacercariae in Cipangopaludina Snails and Adults from Experimentally Infected Animals
    Woon-Mok Sohn, Jong-Yil Chai, Byoung-Kuk Na, Tai-Soon Yong, Keeseon S. Eom, Hansol Park, Duk-Young Min, Han-Jong Rim
    The Korean Journal of Parasitology.2013; 51(2): 191.     CrossRef
  • Topography and ultrastructure of the tegument of Deropristis inflata Molin, 1859 (Digenea: Deropristidae), a parasite of the European eel Anguilla anguilla (Osteichthyes: Anguillidae)
    Jean-José Filippi, Yann Quilichini, Bernard Marchand
    Parasitology Research.2013; 112(2): 517.     CrossRef
  • Topography and ultrastructure of the tegument of Lecithochirium musculus (digenea: Hemiuridae), a parasite of the European eel Anguilla anguilla (osteichthyes: Anguillidae)
    Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Morphology.2012; 273(4): 361.     CrossRef
  • Surface Ultrastructure of Juvenile and Adult Acanthoparyphium tyosenense (Digenea: Echinostomatidae)
    Eun-Taek Han, Moon-Seok Choi, Sung-Yil Choi, Jong-Yil Chai
    Journal of Parasitology.2011; 97(6): 1049.     CrossRef
  • Topography and Ultrastructure of the Tegument of Bucephalus anguillae (Digenea: Bucephalidae), a Parasite of the European Eel Anguilla anguilla (Osteichthyen: Anguillidae)
    Jean-José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Parasitology.2010; 96(6): 1102.     CrossRef
  • Echinostoma caproni: Differential tegumental responses to growth in compatible and less compatible hosts
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  • Centrocestus formosanus (Digenea: Heterophyidae) Encysted in the Freshwater Fish, Puntius brevis, from Lao PDR
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    The Korean Journal of Parasitology.2008; 46(1): 49.     CrossRef
  • Mactra veneriformis, an Intertidal Clam, as a New Second Intermediate Host for Acanthoparyphium marilae (Digenea: Echinostomatidae)
    Eun-Taek Han, Jong-Yil Chai
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  • FIRST RECORD AND DESCRIPTION OF METACERCARIAE OF CURTUTERIA ARGUINAE N. SP. (DIGENEA: ECHINOSTOMATIDAE), PARASITE OF COCKLES CERASTODERMA EDULE (MOLLUSCA: BIVALVIA) IN ARCACHON BAY, FRANCE
    Céline Desclaux, Fernanda Russell-Pinto, Xavier de Montaudouin, Guy Bachelet
    Journal of Parasitology.2006; 92(3): 578.     CrossRef
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Surface ultrastructure of Metagonimus takahashii metacercariae and adults
Jong-Yil Chai, Sang-Mee Guk, Eun-Taek Han, Min Seo, Eun-Hee Shin, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
Korean J Parasitol 2000;38(1):9-15.
Published online March 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.1.9

A scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of Metagonimus takahashii. Metacercariae were collected from the scale of crucian carp (Carassius auratus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker had type I (numerous) and type II (seven in total) sensory papillae. Tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucker, but became sparse and less digitated posteriorly toward the end of the body. In adults, seven type II sensory papillae were characteristically arranged around the lip of the oral sucker, and on the inner side of the lip four small and two large type I sensory papillae were symmetrically seen on each side (12 in total). Tegumental spines on anterior two-thirds of the body, were digitated with 9-12 tips ventrally and 8-13 tips dorsally. Sperms entering into the Laurer's canal were observed. The results show that the surface ultrastructure of M. takahashii is generally similar to those of M. yokogawai and M. miyatai except for the digitation of tegumental spines.

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  • Morphological and molecular characterization of Acanthostomum species with Plesiomonas shigelloides coinfection: Immunological and histopathological appraisal in Bagrus bajad
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    Microbial Pathogenesis.2025; 200: 107365.     CrossRef
  • Body Surface Ultrastructure as a Main Morphological Criterion for Distinguishing Adult Trematode Metagonimus suifunensis
    Polina Shumenko, Yulia Tatonova, Mikhail Shchelkanov
    Biology.2024; 13(11): 942.     CrossRef
  • Digenea

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    Jong Ha Hong, Min Seo, Chang Seok Oh, Jong-Yil Chai, Dong Hoon Shin
    Journal of Parasitology.2020;[Epub]     CrossRef
  • Light microscopy and surface topography of Urotrema scabridum and Renschetrema indicum (Digenea) from Rhinopoma hardwickii (Chiroptera): first report in Egypt
    Kareem Morsy, Fathy Abdel-Ghaffar, Abdel-Rahman Bashtar, Saad Bin Dajem, Rewaida Abdel-Gaber, Marwa Mostafa
    Revista Brasileira de Parasitologia Veterinária.2018;[Epub]     CrossRef
  • Topography and ultrastructure of the tegument of Aphallus tubarium (Rodolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), intestinal parasite of the common Dentex dentex (Linnaeus 1758) from Valinco Gulf
    Laetitia Antonelli, Yann Quilichini, Jos?phine Foata, Bernard Marchand
    Acta Parasitologica.2014;[Epub]     CrossRef
  • Porangatus ceteyusn. gen., n. sp. (Digenea: Zoogonidae): A Parasite ofHoplosternum littorale(Pisces: Callichthyidae) from Amazonas State, Brazil
    Berenice M. M. Fernandes, José C. O. Malta, Aprigio M. Morias
    Comparative Parasitology.2013; 80(2): 203.     CrossRef
  • Topography and ultrastructure of the tegument of Deropristis inflata Molin, 1859 (Digenea: Deropristidae), a parasite of the European eel Anguilla anguilla (Osteichthyes: Anguillidae)
    Jean-José Filippi, Yann Quilichini, Bernard Marchand
    Parasitology Research.2013; 112(2): 517.     CrossRef
  • Topography and ultrastructure of the tegument of Lecithochirium musculus (digenea: Hemiuridae), a parasite of the European eel Anguilla anguilla (osteichthyes: Anguillidae)
    Jean‐José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Morphology.2012; 273(4): 361.     CrossRef
  • Surface Ultrastructure of Juvenile and Adult Acanthoparyphium tyosenense (Digenea: Echinostomatidae)
    Eun-Taek Han, Moon-Seok Choi, Sung-Yil Choi, Jong-Yil Chai
    Journal of Parasitology.2011; 97(6): 1049.     CrossRef
  • Topography and Ultrastructure of the Tegument of Bucephalus anguillae (Digenea: Bucephalidae), a Parasite of the European Eel Anguilla anguilla (Osteichthyen: Anguillidae)
    Jean-José Filippi, Yann Quilichini, Joséphine Foata, Bernard Marchand
    Journal of Parasitology.2010; 96(6): 1102.     CrossRef
  • The life cycle of Ascocotyle (Phagicola) longa (Digenea: Heterophyidae), a causative agent of fish-borne trematodosis
    Susana Balmant Emerique Simões, Helene Santos Barbosa, Cláudia Portes Santos
    Acta Tropica.2010; 113(3): 226.     CrossRef
  • TAXONOMIC STATUS, REDESCRIPTION, AND SURFACE ULTRASTRUCTURE OF ASCOCOTYLE (PHAGICOLA) PINDORAMENSIS N. COMB. (DIGENEA: HETEROPHYIDAE)
    Susana Balmant Emerique Simões, Tomáš Scholz, Helene Santos Barbosa, Cláudia Portes Santos
    Journal of Parasitology.2006; 92(3): 501.     CrossRef
  • Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
    Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
    The Korean Journal of Parasitology.2004; 42(3): 129.     CrossRef
  • Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae)
    Woon-Mok Sohn, Min Seo, Jong-Yil Chai
    The Korean Journal of Parasitology.2003; 41(2): 107.     CrossRef
  • Tegumental ultrastructure of Echinoparyphium recurvatum according to developmental stages
    Woon-Mok Sohn, Ho-Choon Woo, Sung-Jong Hong
    The Korean Journal of Parasitology.2002; 40(2): 67.     CrossRef
  • Surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adult flukes
    Jong-Yil Chai, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
    The Korean Journal of Parasitology.2002; 40(3): 107.     CrossRef
  • Brachylaima cribbi (Digenea: Brachylaimidae): scanning electron microscopical observations of the life-cycle stages
    A.R. Butcher, J.K. Brealey, D.I. Grove, R.B. Dymock
    Journal of Helminthology.2002; 76(3): 207.     CrossRef
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Mini Review

General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics
Ui-Wook Hwang, Won Kim
Korean J Parasitol 1999;37(4):215-228.
Published online December 31, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.4.215

To choose one or more appropriate molecular markers or gene regions for resolving a particular systematic question among the organisms at a certain categorical level is still a very difficult process. The primary goal of this review, therefore, is to provide a theoretical information in choosing one or more molecular markers or gene regions by illustrating general properties and phylogenetic utilities of nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) that have been most commonly used for phylogenetic researches. The highly conserved molecular markers and/or gene regions are useful for investigating phylogenetic relationships at higher categorical levels (deep branches of evolutionary history). On the other hand, the hypervariable molecular markers and/or gene regions are useful for elucidating phylogenetic relationships at lower categorical levels (recently diverged branches). In summary, different selective forces have led to the evolution of various molecular markers or gene regions with varying degrees of sequence conservation. Thus, appropriate molecular markers or gene regions should be chosen with even greater caution to deduce true phylogenetic relationships over a broad taxonomic spectrum.

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Original Article
Surface ultrastructure of Metagonimus miyatai metacercariae and adults
Jong-Yil Chai, Young-Je Kang, Sung-Yil Choi, Sang-Mee Guk, Jae-Ran Yu, Soon-Hyung Lee
Korean J Parasitol 1998;36(4):217-225.
Published online December 20, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.4.217

A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker, and at inner side of the lip one pair of small and two pairs of large type 1 sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four-week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer's canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species, which may be of taxonomic significance.

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