Parasites, Hosts and Diseases

"Trichomonas"

Brief Communications

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(3):329-334.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.329

Abstract
PDF

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

Citations

Citations to this article as recorded by

Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson
Microbial Biotechnology.2025;[Epub] CrossRef
A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis
Zhenke Yang, Jinghui Wang, Yiming Qi, Yiping Shi, Fakun Li, Weijuan Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang
Parasites & Vectors.2024;[Epub] CrossRef
A novel fluoro colorimetric Loop mediated isothermal amplification (LAMP) assay for detection of Trichomonas vaginalis
Shoorashetty Manohar Rudresh, Pareyam Pooja, Pattacheravanda Nanaiah Shakuntala, Kanta Madhu
Indian Journal of Medical Microbiology.2024; 49: 100610. CrossRef
Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen
Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang
Acta Tropica.2024; 260: 107489. CrossRef
Label-free electrochemical DNA biosensing of MR TV 29 18s ribosomal RNA gene of Trichomonas vaginalis by signalization of non-spherical gold nanoparticles
R. Dehdari Vais, H. Heli, N. Sattarahmady
Materials Today Communications.2023; 34: 105123. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
A novel and ultrasensitive label-free electrochemical DNA biosensor for Trichomonas vaginalis detection based on a nanostructured film of poly(ortho-aminophenol)
Rezvan Dehdari Vais, Hossein Heli, Naghmeh Sattarahmady, Afshin Barazesh
Synthetic Metals.2022; 287: 117082. CrossRef
Omics Analyses of Trichomonas vaginalis Actin and Tubulin and Their Participation in Intercellular Interactions and Cytokinesis
Sebastián Lorenzo-Benito, Luis Alberto Rivera-Rivas, Lizbeth Sánchez-Ayala, Jaime Ortega-López, Octavio Montes-Flores, Daniel Talamás-Lara, Rossana Arroyo
Genes.2022; 13(6): 1067. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA
S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady
Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Loop mediated isothermal amplification assay for detection of Trichomonas vaginalis in vaginal swabs among symptomatic women from North India
S. Khurana, R. Dadwal, N. Sharma, A. Mewara, S. Singh, R. Bagga, R. Yadav, S. Sethi
Letters in Applied Microbiology.2020; 70(3): 196. CrossRef
Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Junjun Zhai, Zhang Yan, Feng Ping, Qu Lei, Xuelong Chen, Yanping Qi, Tianwen Wang
PLOS ONE.2020; 15(4): e0230881. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Label-free ultrasensitive electrochemical genosensing of Trichomonas vaginalis using anisotropic-shaped gold nanoparticles as a platform, a repeated sequence of the parasite DNA as a probe, and toluidine blue as a redox marker
N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. Heli
Sensors and Actuators B: Chemical.2018; 273: 234. CrossRef
Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
Shao-Xin Cai, Fan-De Kong, Shu-Fei Xu, Cui-Luan Yao
PeerJ.2018; 6: e5993. CrossRef

10,603 View
196 Download
15 Web of Science
Crossref

Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina: Pamela Testardini, Mar?a Luc?a Gallo Vaulet, Andrea Carolina Entrocassi, Claudia Menghi, Martha Cora Eliseht, Claudia Gatta, Mirta Losada, Mar?a Sol Touz?n, Ana Corominas, Carlos Vay, Silvio Tatti, Angela Famiglietti, Marcelo Rodriguez Fermepin, Beatriz Perazzi; Korean J Parasitol 2016;54(2):191-195.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.191

Abstract
PDF

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.

Citations

Citations to this article as recorded by

Diagnostic accuracy of real-time polymerase chain reaction assay for the detection of Trichomonas vaginalis in clinical samples: A systematic review and meta-analysis
Emmanuel O. Babafemi, Benny P. Cherian, Khalid Rahman, Gilbert M. Mogoko, Oluwatoyin O. Abiola
African Journal of Laboratory Medicine.2025;[Epub] CrossRef
Prevalence and risk factors of Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis infections in pregnant women in seven hospitals in N’Djamena, Chad: a cro
Mahamat Mustapha Anouar, Boris Emmanuel Djoumsie Gomseu, Nandita Sharma, Sana Afreen, Tenzin Tsephel, Djamal Hachim, Ngam Daita, Annour Bechir Ahmat, Mahamat Mouctar Abdelkerim, Aboubakar Hassan Adam, Adam Mahamat Dahabaye, Chatté Adawaye, Foumsou Lhagada
BMJ Open.2025; 15(10): e096775. CrossRef
Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen
Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang
Acta Tropica.2024; 260: 107489. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
The International Society for the Study of Vulvovaginal Disease Vaginal Wet Mount Microscopy Guidelines: How to Perform, Applications, and Interpretation
Pedro Vieira-Baptista, Švitrigailė Grincevičienė, Caroline Oliveira, José Fonseca-Moutinho, Facundo Cherey, Colleen Kennedy Stockdale
Journal of Lower Genital Tract Disease.2021; 25(2): 172. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Análisis microbiológico del tracto genital materno y de la sangre del cordón umbilical en relación con el daño neonatal
Silvina E. Cocucci, Mirtha G. Santacruz Silvero, Mirta O. Losada, María S. Touzón, Hilda RudaVega, Manuel Vazquez Blanco, Sergio L. Provenzano, Carlos A. Vay, Ángela M.R. Famiglietti, Beatriz E. Perazzi
Revista Argentina de Microbiología.2019; 51(2): 157. CrossRef
Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016
Jane Rowley, Stephen Vander Hoorn, Eline Korenromp, Nicola Low, Magnus Unemo, Laith J Abu-Raddad, R Matthew Chico, Alex Smolak, Lori Newman, Sami Gottlieb, Soe Soe Thwin, Nathalie Broutet, Melanie M Taylor
Bulletin of the World Health Organization.2019; 97(8): 548. CrossRef
Very low sensitivity of wet mount microscopy compared to PCR against culture in the diagnosis of vaginal trichomoniasis in Uganda: a cross sectional study
Sheila Nabweyambo, Othman Kakaire, Stefanie Sowinski, Alfred Okeng, Henry Ojiambo, Joshua Kimeze, Irene Najjingo, Freddie Bwanga
BMC Research Notes.2017;[Epub] CrossRef

9,846 View
122 Download
8 Web of Science
Crossref

Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea: Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu; Korean J Parasitol 2016;54(2):187-190.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.187

Abstract
PDF

Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50.

Citations

Citations to this article as recorded by

Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran
Maryam Alikhani, Mohammad Akhoundi, Denis Sereno, Jahangir Abdi, Razi Naserifar, Mohammad Reza Mahmoudi, Asad Mirzaei
Parasitology Research.2022; 121(6): 1631. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef

8,716 View
105 Download
5 Web of Science
Crossref

Original Article

Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis: Sang-Su Kim, Jung-Hyun Kim, Ik-Hwan Han, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2016;54(2):123-132.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.123

Abstract
PDF

Trichomonas vaginalis causes the most prevalent sexually transmitted infection worldwide. Trichomonads have been detected in prostatic tissues from prostatitis, benign prostatic hyperplasia (BPH), and prostate cancer. Chronic prostatic inflammation is known as a risk factor for prostate enlargement, benign prostatic hyperplasia symptoms, and acute urinary retention. Our aim was to investigate whether T. vaginalis could induce inflammatory responses in cells of a benign prostatic hyperplasia epithelial cell line (BPH-1). When BPH-1 cells were infected with T. vaginalis, the protein and mRNA of inflammatory cytokines, such as CXCL8, CCL2, IL-1β, and IL-6, were increased. The activities of TLR4, ROS, MAPK, JAK2/STAT3, and NF-κB were also increased, whereas inhibitors of ROS, MAPK, PI3K, NF-κB, and anti-TLR4 antibody decreased the production of the 4 cytokines although the extent of inhibition differed. However, a JAK2 inhibitor inhibited only IL-6 production. Culture supernatants of the BPH-1 cells that had been incubated with live T. vaginalis (trichomonad-conditioned medium, TCM) contained the 4 cytokines and induced the migration of human monocytes (THP-1 cells) and mast cells (HMC-1 cells). TCM conditioned by BPH-1 cells pretreated with NF-κB inhibitor showed decreased levels of cytokines and induced less migration. Therefore, it is suggested that these cytokines are involved in migration of inflammatory cells. These results suggest that T. vaginalis infection of BPH patients may cause inflammation, which may induce lower urinary tract symptoms (LUTS).

Citations

Citations to this article as recorded by

Immunomodulatory roles of autophagic flux and IFIT in human ectocervical cells upon Trichomonas vaginalis infection
Ching-Chun Liu, Lichieh Julie Chu, Yuan-Ming Yeh, Hsin-Chung Lin, Lih-Chyang Chen, Ching-Yun Huang, Shu-Fang Chiu, Fang-Wen Cheng, Wei-Ning Lin, Kuo-Yang Huang
International Immunopharmacology.2025; 155: 114643. CrossRef
Intrinsic and extrinsic factors causing hyperplasia of the prostate
Yuki Kyoda, Kosuke Shibamori, Tetsuya Shindo, Takeshi Maehana, Kohei Hashimoto, Ko Kobayashi, Toshiaki Tanaka, Fumimasa Fukuta, Naoya Masumori
International Journal of Urology.2024; 31(7): 705. CrossRef
P. gingivalis in oral-prostate axis exacerbates benign prostatic hyperplasia via IL-6/IL-6R pathway
Shuang-Ying Wang, Yi Cai, Xiao Hu, Fei Li, Xin-Hang Qian, Ling-Yun Xia, Bo Gao, Lan Wu, Wen-Zhong Xie, Jia-Min Gu, Tong Deng, Cong Zhu, Hai-Chang Jia, Wan-Qi Peng, Jiao Huang, Cheng Fang, Xian-Tao Zeng
Military Medical Research.2024;[Epub] CrossRef
The correlation between Trichomonas vaginalis infection and reproductive system cancer: a systematic review and meta-analysis
Zhenchao Zhang, Dongxian Li, Yuhua Li, Rui Zhang, Xianghuan Xie, Yi Yao, Linfei Zhao, Xiaowei Tian, Zhenke Yang, Shuai Wang, Xuejing Yue, Xuefang Mei
Infectious Agents and Cancer.2023;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Trichomoniasis
Olivia T. Van Gerwen, Skye A. Opsteen, Keonte J. Graves, Christina A. Muzny
Infectious Disease Clinics of North America.2023; 37(2): 245. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
IL-6 Signaling Link between Inflammatory Tumor Microenvironment and Prostatic Tumorigenesis
Cosmin-Victor Ene, Ilinca Nicolae, Bogdan Geavlete, Petrisor Geavlete, Corina Daniela Ene, Yun Ping Lim
Analytical Cellular Pathology.2022; 2022: 1. CrossRef
Ellagic acid improves benign prostate hyperplasia by regulating androgen signaling and STAT3
Woo Yong Park, Gahee Song, Ja Yeon Park, Kwang Seok Ahn, Hyun Jeong Kwak, Jinbong Park, Jun Hee Lee, Jae-Young Um
Cell Death & Disease.2022;[Epub] CrossRef
Association between trichomoniasis and prostate and bladder diseases: a population-based case–control study
Hung-Yi Yang, Ruei-Yu Su, Chi-Hsiang Chung, Kuo-Yang Huang, Hsin-An Lin, Jui-Yang Wang, Chien-Chou Chen, Wu-Chien Chien, Hsin-Chung Lin
Scientific Reports.2022;[Epub] CrossRef
Increased diagnostic yield of routine multiplex PCR compared to clinician requested testing for detection of Trichomonas vaginalis
Brooke Webb, Andrea Crampton, Michelle J. Francis, John Hamblin, Tony M. Korman, Maryza Graham
Pathology.2021; 53(2): 257. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Inflammatory mediators of prostate epithelial cells stimulated with Trichomonas vaginalis promote proliferative and invasive properties of prostate cancer cells
Ik‐Hwan Han, Jung‐Hyun Kim, Ki‐Seok Jang, Jae‐Sook Ryu
The Prostate.2019; 79(10): 1133. CrossRef
Monocyte-derived extracellular trap (MET) formation induces aggregation and affects motility of human spermatozoa in vitro
Mabel Schulz, Fabiola Zambrano, Hans-Christian Schuppe, Florian Wagenlehner, Anja Taubert, Ulrich Gaertner, Rául Sánchez, Carlos Hermosilla
Systems Biology in Reproductive Medicine.2019; 65(5): 357. CrossRef
Synopsis: Special Issue on “Disruption of signaling homeostasis induced crosstalk in the carcinogenesis paradigmEpistemology of the origin of cancer”
Björn L.D.M. Brücher, Ijaz S. Jamall, Obul R. Bandapalli
4open.2019; 2: 28. CrossRef
Cancer-Associated Fibroblasts Produce Netrin-1 to Control Cancer Cell Plasticity
Pei-Ju Sung, Nicolas Rama, Jeromine Imbach, Stephany Fiore, Benjamin Ducarouge, David Neves, Huei-Wen Chen, David Bernard, Pan-Chyr Yang, Agnès Bernet, Stephane Depil, Patrick Mehlen
Cancer Research.2019; 79(14): 3651. CrossRef
Atractylenolide II Induces Apoptosis of Prostate Cancer Cells through Regulation of AR and JAK2/STAT3 Signaling Pathways
Jing Wang, Moussa Ide Nasser, Salah Adlat, Ming Ming Jiang, Nan Jiang, Li Gao
Molecules.2018; 23(12): 3298. CrossRef
Proliferation of prostate epithelia induced by IL‐6 from stroma reacted with Trichomonas vaginalis
J.‐H. Kim, I.‐H. Han, Y.‐S. Kim, C.‐S. Noh, J.‐S. Ryu
Parasite Immunology.2018;[Epub] CrossRef
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated WithTrichomonas vaginalisvia Crosstalk With Mast Cell
Jung-Hyun Kim, Sang-Su Kim, Ik-Hwan Han, Seobo Sim, Myoung-Hee Ahn, Jae-Sook Ryu
The Prostate.2016; 76(15): 1431. CrossRef
Signalling pathways associated with IL‐6 production and epithelial–mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis
I. H. Han, J. H. Kim, S. S. Kim, M. H. Ahn, J. S. Ryu
Parasite Immunology.2016; 38(11): 678. CrossRef

13,910 View
178 Download
22 Web of Science
Crossref

Brief Communications

Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(1):75-80.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.75

Abstract
PDF

This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR detected T. vaginalis in 12 (1.9%) patients. Testing via PCR demonstrated high sensitivity and high negative predictive value for T. vaginalis. Among the 19 women who tested positive for T. vaginalis according to PCR, 94.7% (18/19) reported vaginal signs and symptoms. Notably, more than 50% of T. vaginalis infections occurred in females younger than 30 years old, and 58% were unmarried. Multiplex PCR, which simultaneously detects pathogens from various sexually transmitted infections, revealed that 91.7% (11/12) of patients were infected with 2 or more pathogens. Mycoplasma hominis was the most prevalent co-infection pathogen with T. vaginalis, followed by Ureaplasma urealyticum and Chlamydia trachomatis. Our results indicate that PCR and multiplex PCR are the most sensitive tools for T. vaginalis diagnosis, rather than microscopy which has been routinely used to detect T. vaginalis infections in South Korea. Therefore, clinicians should take note of the high prevalence of T. vaginalis infections among adolescent and young women in order to prevent persistent infection and transmission of this disease.

Citations

Citations to this article as recorded by

Diagnostic accuracy of real-time polymerase chain reaction assay for the detection of Trichomonas vaginalis in clinical samples: A systematic review and meta-analysis
Emmanuel O. Babafemi, Benny P. Cherian, Khalid Rahman, Gilbert M. Mogoko, Oluwatoyin O. Abiola
African Journal of Laboratory Medicine.2025;[Epub] CrossRef
The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis
Zeinab Moghadamizad, Javad Yazdizadeh Khalili, Meysam Olfatifar, Milad Badri, Sasan Khazaei
International Health.2024; 16(3): 240. CrossRef
Combination of genitourinary candidiasis and sexually transmitted infections
N.E. Portnyagina, A.K. Kvardakova, V.V. Pakhomova, E.G. Gubanova, N.V. Deeva, I.G. Sergeeva
Russian Journal of Clinical Dermatology and Venereology.2024; 23(4): 377. CrossRef
Trichomonas vaginalis: comparison of primers for implementation as an in-house PCR in rural Vellore, South India
Nagarajan L. Surya, Thangamani Suji, Santhosh Rani, Irene Dorathy, Shantidani Minz, Rani Diana Sahni
BMC Infectious Diseases.2024;[Epub] CrossRef
Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Comparison of Diagnostic Methods for Detection of Trichomonas vaginalis in Prediagnosed Vaginitis Cases and Its Association with Various Pathogens
Vildan Turan Faraşat, İbrahim Cüneyt Balcıoğlu, Pınar Solmaz Hasdemir, Ertaç Gümüş
Turkish Journal of Parasitology.2022; 46(3): 167. CrossRef
Trichomonas vaginalis follow-up and persistence in Colombian women
Lauren Hernández-Buelvas, Milena Camargo, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
Scientific Reports.2021;[Epub] CrossRef
Trichomoniasis in a tertiary hospital of Madrid, Spain (2013–2017): prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis
Celia Bolumburu, Vega Zamora, María Muñoz-Algarra, Francisca Portero-Azorín, José Antonio Escario, Alexandra Ibáñez-Escribano
Parasitology Research.2020; 119(6): 1915. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
PREVALENCE OF TRICHOMONIASIS IN ASYMPTOMATIC PREGNANT WOMEN POPULATION IN BANDUNG, WEST JAVA, INDONESIA
Pati Aji Achdiat, Reiva Farah Dwiyana, Vina Feriza, Rasmia Rowawi, Rendy Ariezal Effendi, Oki Suwarsa, Hendra Gunawan
Indonesian Journal of Tropical and Infectious Disease.2019; 7(4): 57. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(3): 329. CrossRef
Epidemiology of Trichomoniasis in South Korea and Increasing Trend in Incidence, Health Insurance Review and Assessment 2009-2014
So-Young Joo, Youn-Kyoung Goo, Jae-Sook Ryu, Sang-Eun Lee, Won Kee Lee, Dong-Il Chung, Yeonchul Hong, Zhefeng Meng
PLOS ONE.2016; 11(12): e0167938. CrossRef

11,076 View
150 Download
11 Web of Science
Crossref

Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis: Hyun-Ouk Song; Korean J Parasitol 2016;54(1):71-74.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.71

Abstract
PDF

Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.

Citations

Citations to this article as recorded by

Trichomonas vaginalis acid sphingomyelinases' theoretical structural analysis shows substrate binding diversity related to protein flexibility and mobility
Ana Laura Medina-Nieto, Sairy Yarely Andrade-Guillen, Fátima Berenice Ramírez-Montiel, Fátima Tornero-Gutiérrez, José A. Martínez-Álvarez, Ángeles Rangel-Serrano, Itzel Páramo-Pérez, Naurú Idalia Vargas-Maya, Javier de la Mora, Claudia Leticia Mendoza-Mac
Computational Biology and Chemistry.2026; 120: 108601. CrossRef
Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites
Namrata Anand
Frontiers in Parasitology.2024;[Epub] CrossRef
Genetic Variability of the Internal Transcribed Spacer and Pyruvate:Ferredoxin Oxidoreductase Partial Gene of Trichomonas vaginalis from Female Patients
Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocaña, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos
Microorganisms.2023; 11(9): 2240. CrossRef
Pyruvate: Ferredoxin oxidoreductase is involved in IgA-related microbiota dysbiosis and intestinal inflammation
Kairuo Wang, Yixuan Guo, Yuanyuan Liu, Xiao Cui, Xiang Gu, Lixiang Li, Yanqing Li, Ming Li
Frontiers in Immunology.2022;[Epub] CrossRef
Microbiota in vaginal health and pathogenesis of recurrent vulvovaginal infections: a critical review
Namarta Kalia, Jatinder Singh, Manpreet Kaur
Annals of Clinical Microbiology and Antimicrobials.2020;[Epub] CrossRef
New light on ancient enzymes – in vitro CO2 Fixation by Pyruvate Synthase of Desulfovibrio africanus and Sulfolobus acidocaldarius
Andreas Witt, Roberta Pozzi, Stephan Diesch, Oliver Hädicke, Hartmut Grammel
The FEBS Journal.2019; 286(22): 4494. CrossRef
Humoral and T cell–mediated immune response against trichomoniasis
M. Nemati, N. Malla, M. Yadav, H. Khorramdelazad, A. Jafarzadeh
Parasite Immunology.2018;[Epub] CrossRef
A Cell Surface Aggregation-Promoting Factor from Lactobacillus gasseri Contributes to Inhibition of Trichomonas vaginalis Adhesion to Human Vaginal Ectocervical Cells
Niha Phukan, Anna E. S. Brooks, Augusto Simoes-Barbosa, John H. Adams
Infection and Immunity.2018;[Epub] CrossRef
Can the pyruvate: ferredoxin oxidoreductase (PFOR) gene be used as an additional marker to discriminate among Blastocystis strains or subtypes?
Patricia Alarcon-Valdes, Guiehdani Villalobos, Williams Arony Martinez-Flores, Eduardo Lopez-Escamilla, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Fernando Martinez-Hernandez, Jonnathan Guadalupe Santillan-Benitez, Pablo Maravilla
Parasites & Vectors.2018;[Epub] CrossRef
Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins
Dong Liu, Zhengjiao Yang, Yong Chen, Wei Zhuang, Huanqing Niu, Jinglan Wu, Hanjie Ying
Biotechnology for Biofuels.2018;[Epub] CrossRef

9,622 View
93 Download
10 Web of Science
Crossref

Original Articles

Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products: Giimaa Narantsogt, Arim Min, Young Hee Nam, Young Ah Lee, Kyeong Ah Kim, Gurbadam Agvaandaram, Temuulen Dorjsuren, Jamel El-Benna, Myeong Heon Shin; Korean J Parasitol 2015;53(5):597-603.
Published online October 29, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.5.597

Abstract
PDF

Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47^phox in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.

Citations

Citations to this article as recorded by

Dynamin 2-mediated endocytosis of BLT1 is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 281. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Latent Upregulation of Nlrp3, Nlrc4 and Aim2 Differentiates between Asymptomatic and Symptomatic Trichomonas vaginalis Infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Immunological Investigations.2022; 51(5): 1127. CrossRef
Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species
Juan-Hua Quan, Byung-Hun Kang, Jung-Bo Yang, Yun-Ee Rhee, Heung-Tae Noh, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
BioMed Research International.2017; 2017: 1. CrossRef
SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete
Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton
Infection and Immunity.2017;[Epub] CrossRef
Explore the variation of MMP3, JNK, p38 MAPKs, and autophagy at the early stage of osteoarthritis
Jie Shi, Changjie Zhang, Zhongjie Yi, Chunna Lan
IUBMB Life.2016; 68(4): 293. CrossRef

10,406 View
109 Download
7 Web of Science
Crossref

Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with Trichomonas vaginalis: Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee; Korean J Parasitol 2015;53(4):371-377.
Published online August 25, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.4.371

Abstract
PDF

Trichomonas vaginalis induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in TNF-α production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased TNF-α production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, TNF-α production was significantly decreased compared to the control; however, TNF-α reduction patterns were different depending on the type of PI3K/MAPK inhibitors. TNF-α production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of TNF-α production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.

Citations

Citations to this article as recorded by

Exploring the therapeutic potential and in vitro validation of baicalin for the treatment of triple-negative breast cancer
Yuan Ma, Ying Pan, Qiancheng Zhao, Chongheng Zhang, Haitao He, Lihua Pan, Jianling Jia, Aiping Shi, Yiming Yang, Wenfeng Zhang
Frontiers in Pharmacology.2025;[Epub] CrossRef
PI3K/AKT signaling in parasites and parasite diseases: Role and therapeutic potential
Lujun Yan, Yating Li, Xing Yang, Rui Li, Chunyin Zhu, Xuedong He, Xiaoliang Jin, Guanghui Zheng, Naunain Mehmood, William C. Cho, Shijun Bao, Houhui Song, Yadong Zheng
Virulence.2025;[Epub] CrossRef
Interactions between bacterial vaginosis-associated microbiota and Trichomonas vaginalis modulate parasite-induced pathogenicity and host immune responses
Shu-Fang Chiu, Ching-Yun Huang, Chien-Yung Chen, Wei-Jane Hsu, Yuan-Ming Yeh, Ya-Wen Shih, Lichieh Julie Chu, Wei-Ning Lin, Kuo-Yang Huang
Parasites & Vectors.2025;[Epub] CrossRef
Therapeutic effects of coptisine derivative EHLJ7 on colorectal cancer by inhibiting PI3K/AKT pathway
ChangWei Chai, XiaoNan Tang, XiaoQian Chi, Xiang Li, HaiJing Zhang, LianQiu Wu
Cellular Signalling.2024; 116: 111053. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
Chemokine CXCL10 regulates pain behaviors via PI3K-AKT signaling pathway in mice
Yan Fang, Xiaoling Peng, Huilian Bu, Xiaoqian Jia, Feng Gao, Cheng Liu
Neuropeptides.2022; 93: 102243. CrossRef
The role of TNF-α induced protein 1 in the activation of pro-apoptotic proteins
Xiaoren Tang, Thanarut Tangkham, Bushra Aljahdali, Sean Lee, Mingfang Su, Serge Dibart
Human Cell.2021; 34(4): 1123. CrossRef
Prevention of EHLJ7 on Colitis-Associated Colorectal Cancer Tumorigenesis Through Regulating PI3K/AKT Pathway
XiaoNan Tang, ChangWei Chai, Ying Guan, Xiang Li, AnJun Deng, HaiLin Qin, HaiJing Zhang, Lianqiu Wu
SSRN Electronic Journal .2021;[Epub] CrossRef
LncRNA MNX1‐AS1 promotes the progression of cervical cancer through activating MAPK pathway
Xiang Liu, Qian Yang, Jinyu Yan, Xiahui Zhang, Meiyun Zheng
Journal of Cellular Biochemistry.2019; 120(3): 4268. CrossRef
Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-κB Pathways Partially Mediated by TLR2
Ling Li, Xin Li, Pengtao Gong, Xichen Zhang, Zhengtao Yang, Ju Yang, Jianhua Li
Frontiers in Microbiology.2018;[Epub] CrossRef
Mapping out p38MAPK
Elizabeth A. Bonney
American Journal of Reproductive Immunology.2017;[Epub] CrossRef
C5a Induces the Synthesis of IL-6 and TNF-α in Rat Glomerular Mesangial Cells through MAPK Signaling Pathways
Mingde Ji, Yanlai Lu, Chenhui Zhao, Wenxing Gao, Fengxia He, Jing Zhang, Dan Zhao, Wen Qiu, Yingwei Wang, Hiroyasu Nakano
PLOS ONE.2016; 11(9): e0161867. CrossRef

9,268 View
92 Download
12 Web of Science
Crossref

Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells: Juan-Hua Quan, In-Wook Choi, Jung-Bo Yang, Wei Zhou, Guang-Ho Cha, Yu Zhou, Jae-Sook Ryu, Young-Ha Lee; Korean J Parasitol 2014;52(6):595-603.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.595

Abstract
PDF

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.

Citations

Citations to this article as recorded by

Chromatin accessibility and gene expression in the parasite Trichomonas vaginalis
Agustina Prat, Daniela Muñoz, Ayelen Lizarraga, Julieta Seifert-Gorzycki, Estefania Sanchez-Vazquez, Patricia J. Johnson, Pablo H. Strobl-Mazzulla, Natalia de Miguel
BMC Infectious Diseases.2025;[Epub] CrossRef
Metallopeptidases as Key Virulence Attributes of Clinically Relevant Protozoa: New Discoveries, Perspectives, and Frontiers of Knowledge
Graziela Vargas Rigo, Fernanda Gomes Cardoso, Giulia Bongiorni Galego, Deisiane Fernanda da Rosa, André Luis Souza dos Santos, Tiana Tasca
Current Protein & Peptide Science.2023; 24(4): 307. CrossRef
VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles
Nehuén Salas, Veronica M. Coceres, Tuanne dos Santos Melo, Antonio Pereira-Neves, Vanina G. Maguire, Tania M. Rodriguez, Bruna Sabatke, Marcel I. Ramirez, Jihui Sha, James A. Wohlschlegel, Natalia de Miguel
Cellular and Molecular Life Sciences.2022;[Epub] CrossRef
Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis-Induced Cell Detachment
Bénédicte Pradines, Séverine Domenichini, Vanessa Lievin-Le Moal
Pharmaceuticals.2022; 15(11): 1350. CrossRef
The 50 kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor
Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Al
Molecular and Biochemical Parasitology.2017; 217: 32. CrossRef
Regulation of exosomes released from normal ovarian epithelial cells and ovarian cancer cells
Wei Zhang, Jiaxin Yang, Dongyan Cao, Yan You, Keng Shen, Peng Peng
Tumor Biology.2016; 37(12): 15763. CrossRef
Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with <i>Trichomonas vaginalis</i>
Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(4): 371. CrossRef

11,446 View
103 Download
7 Web of Science
Crossref

Brief Communication

Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea: Jun-Hyeok Seo, Hye-Won Yang, So-Young Joo, Su-Min Song, Yu-Ran Lee, Jae-Sook Ryu, Eun Sang Yoo, Won Kee Lee, Hyun-Hee Kong, Sang-Eun Lee, Won-Ja Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(5):551-555.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.551

Abstract
PDF

Trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. Despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including PCR, few prospective studies present data on male T. vaginalis infection in South Korea. In the present study, the prevalence of T. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary care urology clinic in South Korea. The prevalence of T. vaginalis infection in our cohort was 4% (8/201) by PCR. T. vaginalis infection was common in men older than 40 years (median age, 52 years). Among the 8 Trichomonas-positive patients, 87.5% (7/8) had prostatic diseases, such as prostatitis and benign prostatic hyperplasia, and 25.0% (2/8) and 12.5% (1/8) were coinfected with Chlamydia trachomatis and Mycoplasma genitalium, respectively. Our results suggest that T. vaginalis infection is not rare in men attending primary care urology clinics in South Korea, especially in those older than 40 years, in whom it may explain the presence of prostatic disease. The possibility of T. vaginalis infection should be routinely considered in older male patients with prostatic diseases in South Korea.

Citations

Citations to this article as recorded by

Trichomoniasis in Men: A Neglected Factor in Male Infertility?
Seyed Ali Hosseini, Mohammad Matini, Maryam Bahmanzadeh, Reza Aslani, Faeze Foroughi-Parvar
Acta Parasitologica.2025;[Epub] CrossRef
Systematic review and meta-analysis of the global prevalence and infection risk factors of Trichomonas vaginalis
Wenjie Tian, Yuhua Li, Yani Zhang, Yiming Zhang, Yiran Qin, Yalin Han, Dongxian Li, Shuai Wang, Zhenke Yang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang
Parasite.2025; 32: 56. CrossRef
Prevalence of trichomoniasis infection among adults in Nigerian community settings
K. Chukwuemeka Obetta, Innocent Okonkwo Ogbonna, Dorida Nneka Oyigbo, Oliver Onyemaechi Ugwu, Kenneth Okonkwo Ugwu, Beatrice N. Onah, Chinasa Maryrose Ugwunnadi, Joseph O. Acha, Ngozi Uzoamaka Chuke, Ogechi Nkemjika, Onyinyechi Elizabeth Okoye
Medicine.2023; 102(37): e34585. CrossRef
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China
Zhenchao Zhang, Yuhui Sang, Pucheng Wu, Yujia Shang, Lesong Li, Yujuan Duan, Linfei Zhao, Minghui Gao, Lihua Guo, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lixia Hao, Xuefang Mei, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Urogenital Trichomonas vaginalis infection in males: a case report and retrospective analysis of a 10‐year period in a tertiary hospital
Miguel Alpalhão, Luís Marques‐Lito, Paulo Filipe, João Borges‐Costa
International Journal of Dermatology.2022;[Epub] CrossRef
Seroprevalence and risk factors of Trichomonas vaginalis among couples in Al-Hamza city-Iraq.
Musafer H. Al-Ardi
Al-Kufa University Journal for Biology.2021; 13(1): 26. CrossRef
Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men
Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park
The Korean Journal of Parasitology.2019; 57(1): 21. CrossRef
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2019; 57(1): 27. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(1): 75. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef

11,537 View
127 Download
13 Web of Science
Crossref

Original Article

Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia: Mudyawati Kamaruddin, Masaharu Tokoro, Md. Moshiur Rahman, Shunsuke Arayama, Anggi P.N. Hidayati, Din Syafruddin, Puji B.S. Asih, Hisao Yoshikawa, Ei Kawahara; Korean J Parasitol 2014;52(5):471-478.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.471

Abstract
PDF

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.

Citations

Citations to this article as recorded by

18S rRNA gene metabarcoding for investigation of gastrointestinal parasite diversity in great cormorants
Subin Lee, Badriah Alkathiri, Chang Hyeon Lee, Heon Woo Lee, Dong-Hyuk Jeong, Ju Yeong Kim, Seongjun Choe, Seung-Hun Lee
Scientific Reports.2025;[Epub] CrossRef
Spatial composition differences and diversity of gut eukaryotes in white-lipped deer in Xizang, China
Yuangang Yang, Wei Luo, Peng Luo, Mingkun He, Feng Jiang, Jianli Xiong, Zhangqiang You
Zoologia (Curitiba).2025;[Epub] CrossRef
Development of a novel one-tube LAMP-CRISPR/Cas12b technique for detecting Pentatrichomonas hominis
Qing-Xin Fan, Jin-Long Wang, Yao Liang, Yu-Xuan Wang, Han-Dan Xiao, Xing-Quan Zhu, Qing Liu
Microchemical Journal.2025; 215: 114488. CrossRef
Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single‐Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as ‘Non‐Pathogenic’
Christen Rune Stensvold
APMIS.2025;[Epub] CrossRef
Prevalence of intestinal trichomonads in captive non-human primates in China
Ping-Ping Ma, Yang Zou, Wen-Jie Mu, Yue-Yue Zhang, Ya-Qi Li, Zhong-Li Liu, Long Zhang, Li-Xian Chen, Guo-Hua Liu, Shuai Wang
Parasite.2024; 31: 19. CrossRef
Development of an LFD-RPA Assay for Rapid Detection of Pentatrichomonas hominis Infection in Dogs
Yao Rong, Xichen Zhang, Xuejiao Chen, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Taotao Yue, Hongbo Zhang, Xiaofei Zhou, Nan Zhang
Current Issues in Molecular Biology.2023; 45(11): 9252. CrossRef
Prevalence and molecular characterization of Pentatrichomonas hominis in Siberian tigers (Panthera tigris altaica) in northeast China
Hongbo ZHANG, Nan ZHANG, Pengtao GONG, Shuqin CHENG, Xiaocen WANG, Xin LI, Zhijun HOU, Chang LIU, Tianqi BI, Bobo WANG, Yidan CHENG, Jianhua LI, Xichen ZHANG
Integrative Zoology.2022; 17(4): 543. CrossRef
Pentatrichomonas hominis and other intestinal parasites in school-aged children: coproscopic survey
Sarah Mohamed Abdo, Marwa Mohamed Ibrahim Ghallab, Nagwa Mohammed Elhawary, Heba Elhadad
Journal of Parasitic Diseases.2022; 46(3): 896. CrossRef
Occurrence and Genetic Diversity of the Zoonotic Enteric Protozoans and Enterocytozoon bieneusi in Père David’s Deer (Elaphurus davidianus) from Beijing, China
Peiyang Zhang, Qingxun Zhang, Shuyi Han, Guohui Yuan, Jiade Bai, Hongxuan He
Pathogens.2022; 11(11): 1223. CrossRef
Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand
Aongart Mahittikorn, Ruenruetai Udonsom, Khuanchai Koompapong, Rachatawan Chiabchalard, Chantira Sutthikornchai, Preeyaporn Monatrakul Sreepian, Hirotake Mori, Supaluk Popruk
BMC Veterinary Research.2021;[Epub] CrossRef
Prevalence of Tetratrichomonas buttreyi and Pentatrichomonas hominis in yellow cattle, dairy cattle, and water buffalo in China
Wen-Chao Li, Jia-min Huang, Zhui Fang, Qi Ren, Li Tang, Zhen-zhen Kan, Xin-chao Liu, You-fang Gu
Parasitology Research.2020; 119(2): 637. CrossRef
Protozoan genital invasions caused by the representatives of trichomonas and giardia
Pavlo V. Fedorych, Gennadiy I. Mavrov, Tetiana V. Osinska, Yuliia V. Shcherbakova
Wiadomości Lekarskie.2020; 73(2): 380. CrossRef
Molecular prevalence of trichomonad species from pet shop puppies and kittens in Japan
Naoyuki Itoh, Yuko Iijima, Isao Ogura, Natsumi Yonekura, Satoshi Kameshima, Yuya Kimura
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Three monophyletic clusters in Retortamonas species isolated from vertebrates
Joko Hendarto, Tetsushi Mizuno, Anggi P.N. Hidayati, Ismail E. Rozi, Puji B.S. Asih, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Masaharu Tokoro
Parasitology International.2019; 69: 93. CrossRef
Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia
Takahiro Matsumura, Joko Hendarto, Tetsushi Mizuno, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Taro Nishimura, Masaharu Tokoro
Tropical Medicine and Health.2019;[Epub] CrossRef
Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid
Chao Lin, Furong Ying, Yanan Lai, Xiaolong Li, Xiangyang Xue, Tieli Zhou, Dongwei Hu
BMC Infectious Diseases.2019;[Epub] CrossRef
A survey of gastrointestinal parasites of goats in a goat market in Kathmandu, Nepal
Tirth Raj Ghimire, Namita Bhattarai
Journal of Parasitic Diseases.2019; 43(4): 686. CrossRef
High prevalence of Pentatrichomonas hominis infection in gastrointestinal cancer patients
Nan Zhang, Hongbo Zhang, Yanhui Yu, Pengtao Gong, Jianhua Li, Ziyi Li, Ting Li, Zhanjie Cong, Chunying Tian, Xiaofeng Liu, Xiuyan Yu, Xichen Zhang
Parasites & Vectors.2019;[Epub] CrossRef
Meta-taxonomic analysis of prokaryotic and eukaryotic gut flora in stool samples from visceral leishmaniasis cases and endemic controls in Bihar State India
Rachael Lappan, Cajsa Classon, Shashi Kumar, Om Prakash Singh, Ricardo V. de Almeida, Jaya Chakravarty, Poonam Kumari, Sangeeta Kansal, Shyam Sundar, Jenefer M. Blackwell, Guy Caljon
PLOS Neglected Tropical Diseases.2019; 13(9): e0007444. CrossRef
Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China
Wen Chao Li, Kai Wang, Youfang Gu
Parasites & Vectors.2018;[Epub] CrossRef
Simplicimonas- like DNA in vaginal swabs of cows and heifers cross-reacting in the real-time PCR for T. foetus
Caroline F. Frey, Norbert Müller, Norbert Stäuber, Nelson Marreros, Larissa Hofmann, Brigitte Hentrich, Gaby Hirsbrunner
Veterinary Parasitology.2017; 237: 30. CrossRef
Report of the first clinical case of intestinal trichomoniasis caused by Tritrichomonas foetus in a cat with chronic diarrhoea in Brazil
Aline S. Hora, Samantha I. Miyashiro, Fabiana C. Cassiano, Paulo E. Brandão, Archivaldo Reche-Junior, Hilda F. J. Pena
BMC Veterinary Research.2017;[Epub] CrossRef
Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay
Douglas Chan, Joel Barratt, Tamalee Roberts, Owen Phillips, Jan Šlapeta, Una Ryan, Deborah Marriott, John Harkness, John Ellis, Damien Stark
Veterinary Parasitology.2016; 227: 42. CrossRef
Human Blastocystis subtyping with subtype-specific primers developed from unique sequences of the SSU rRNA gene
Hisao Yoshikawa, Ayana Iwamasa
Parasitology International.2016; 65(6): 785. CrossRef
Molecular and morphological diversity of the genus Hypotrichomonas (Parabasalia: Hypotrichomonadida), with descriptions of six new species
Vít Céza, Tomáš Pánek, Pavla Smejkalová, Ivan Čepička
European Journal of Protistology.2015; 51(2): 158. CrossRef

13,726 View
153 Download
22 Web of Science
Crossref

Brief Communications

Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis: Hyun-Ouk Song, Jae-Sook Ryu; Korean J Parasitol 2013;51(4):479-484.
Published online August 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.4.479

Abstract
PDF

Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O₂^.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis.

Citations

Citations to this article as recorded by

IDO1 promotes Echinococcus multilocularis infection by regulating the formation of neutrophil extracellular traps
Haining Zhang, Ru Meng, Fan Zhang, Ao Chen, Hongrun Ge, Wangkai Chen, Zhi Li, Yong Fu
Veterinary Research.2025;[Epub] CrossRef
Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth
María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila
Parasitology Research.2022; 121(5): 1355. CrossRef
Immunological Activities of Crude Polysaccharides Extracts ofClimacodon pulcherrimus(Phanerochaetaceae) in Lipopolysaccharide-Induced Rat Macrophages and Neutrophils' Responses
Tume Christopher, Oumar Mahamat, Miyanui N. Chungong, C. Atemenkeh Ngwa, P. Maishu Samka
Journal of Medicinal Food.2021; 24(2): 180. CrossRef
Angiotensin‐converting enzyme as a new immunologic target for the new SARS‐CoV‐2
Shahin Hallaj, Anahita Ghorbani, Seyed Ali Mousavi‐Aghdas, Mohammad Mirza‐Aghazadeh‐Attari, Andrey Sevbitov, Vida Hashemi, Tooba Hallaj, Farhad Jadidi‐Niaragh
Immunology & Cell Biology.2021; 99(2): 192. CrossRef
The state of art of neutrophil extracellular traps in protozoan and helminthic infections
César Díaz-Godínez, Julio C. Carrero
Bioscience Reports.2019;[Epub] CrossRef
Recombinant α-actinin subunit antigens of Trichomonas vaginalis as potential vaccine candidates in protecting against trichomoniasis
Yi-Ting Xie, Jiang-Mei Gao, Ya-Ping Wu, Petrus Tang, Geoff Hide, De-Hua Lai, Zhao-Rong Lun
Parasites & Vectors.2017;[Epub] CrossRef
Adenosine reduces reactive oxygen species and interleukin-8 production by Trichomonas vaginalis-stimulated neutrophils
Amanda Piccoli Frasson, Camila Braz Menezes, Gustavo Krumel Goelzer, Simone Cristina Baggio Gnoatto, Solange Cristina Garcia, Tiana Tasca
Purinergic Signalling.2017; 13(4): 569. CrossRef

9,464 View
71 Download
Crossref

The Dimension of Trichomonas vaginalis as Measured by Scanning Electron Microscopy: Sang-Hoon Cheon, Seung Ryong Kim, Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2013;51(2):243-246.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.243

Abstract
PDF

It is known that physicochemical conditions (e.g., pH, temperature, and ionic strength) affect the size of trichomonads. In this study, the sizes of 4 isolates of Trichomonas vaginalis cultured for more than a year (called "old T") and 3 isolates freshly isolated from vaginitis cases (called "fresh T") were compared by scanning electron microscopy. Although the fresh T had shorter body length, body width, and flagellar length than old T, total length (about 26 ?m), including body length, flagella length, and axostyle length was almost the same in the 2 groups. A striking difference was observed between the axostyles of the 2 groups; the axostyle length of the fresh T (8.2 ?m) was more than twice as long as that of the old T (4.0 ?m). However, in several parasitology textbooks, the length of T. vaginalis is said to vary widely from 7 to 32 ?m, and its undulating membrane is said to extend about half way (53.5%) to the posterior end of the body. On the other hand, in our study, the undulating membrane was observed to extend more than 3/4 of the body length (72.1%) in old T, whereas in fresh T it could not be measured. Taken together, we suggest that T. vaginalis averages 26 (21-32) ?m in total length, with 9.5 (7.4-11.4) ?m of body length and 6.8 (5.3-7.7) ?m of width, and its undulating membrane extending 3/4 of its body length. Therefore, these findings may provide useful information for morphological characteristics of T. vaginalis.

Citations

Citations to this article as recorded by

In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis
Fernanda Gomes Cardoso, Luisa Trindade dos Santos, Saulo Almeida Menezes, Graziela Vargas Rigo, Tiana Tasca
Frontiers in Parasitology.2025;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Neutrophil interactions with the sexually transmitted parasite Trichomonas vaginalis: implications for immunity and pathogenesis
Suhani B. Bhakta, Jose A. Moran, Frances Mercer
Open Biology.2020;[Epub] CrossRef
Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis
Frances Mercer, Shek Hang Ng, Taylor M. Brown, Grace Boatman, Patricia J. Johnson, Fred Hughson
PLOS Biology.2018; 16(2): e2003885. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef

11,388 View
86 Download
Crossref

Efficacy of Ronidazole for Treatment of Cats Experimentally Infected with a Korean Isolate of Tritrichomonas foetus: Sun Lim, Sang-Ik Park, Kyu-Sung Ahn, Dae-Sung Oh, Sung-Shik Shin; Korean J Parasitol 2012;50(2):161-164.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.161

Abstract
PDF

To evaluate the efficacy of ronidazole for treatment of Tritrichomonas foetus infection, 6 Tritrichomonas-free kittens were experimentally infected with a Korean isolate of T. foetus. The experimental infection was confirmed by direct microscopy, culture, and single-tube nested PCR, and all cats demonstrated trophozoites of T. foetus by day 20 post-infection in the feces. From day 30 after the experimentally induced infection, 3 cats were treated with ronidazole (50 mg/kg twice a day for 14 days) and 3 other cats received placebo. Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks. To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay. All 3 cats in the treatment group receiving ronidazole showed negative results for T. foetus infection during 2 weeks of treatment and 4 weeks follow-up by all detection methods used in this study. In contrast, rectal swab samples from cats in the control group were positive for T. foetus continuously throughout the study. The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

Citations

Citations to this article as recorded by

Metabarcoding of pathogenic parasites based on copro-DNA analysis of wild animals in South Korea
Jun Ho Choi, Soo Lim Kim, Dong Kyun Yoo, Myung-hee Yi, Singeun Oh, Myungjun Kim, Sohyeon Yun, Tai-Soon Yong, Seongjun Choe, Jong Koo Lee, Ju Yeong Kim
Heliyon.2024; 10(9): e30059. CrossRef
Efficacy of guar gum-based ronidazole capsules as a treatment for Tritrichomonas foetus infection in cats
Aurélien Grellet, Seyf Eddine Makhlouf, Loic Desquilbet, Fani Hovhannessian, Cassandre Boogaerts, Vanessa Dore, Myriam Anthony, Bernadette Espana, Caroline Prouillac, Plamen Kirilov, Bruno Polack, Sébastien Perrot
Journal of Feline Medicine and Surgery.2017; 19(2): 177. CrossRef
The conundrum of feline trichomonosis: the more we learn the ‘trickier’ it gets
Jody L Gookin, Katherine Hanrahan, Michael G Levy
Journal of Feline Medicine and Surgery.2017; 19(3): 261. CrossRef

8,155 View
80 Download
Crossref

PCR for Diagnosis of Male Trichomonas vaginalis Infection with Chronic Prostatitis and Urethritis: Jong Jin Lee, Hong Sang Moon, Tchun Yong Lee, Hwan Sik Hwang, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2012;50(2):157-159.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.157

Abstract
PDF

The aim of this study was to assess the usefulness of PCR for diagnosis of Trichomonas vaginalis infection among male patients with chronic recurrent prostatitis and urethritis. Between June 2001 and December 2003, a total of 33 patients visited the Department of Urology, Hanyang University Guri Hospital and were examined for T. vaginalis infection by PCR and culture in TYM medium. For the PCR, we used primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Voided bladder urine (VB1 and VB3) was sampled from 33 men with symptoms of lower urinary tract infection (urethral charge, residual urine sensation, and frequency). Culture failed to detect any T. vaginalis infection whereas PCR identified 7 cases of trichomoniasis (21.2%). Five of the 7 cases had been diagnosed with prostatitis and 2 with urethritis. PCR for the 5 prostatitis cases yielded a positive 330 bp band from bothVB1 and VB3, whereas positive results were only obtained from VB1 for the 2 urethritis patients. We showed that the PCR method could detect T. vaginalis when there was only 1 T. vaginalis cell per PCR mixture. Our results strongly support the usefulness of PCR on urine samples for detecting T. vaginalis in chronic prostatitis and urethritis patients.

Citations

Citations to this article as recorded by

Trikomoniyaz tanısında farklı laboratuvar yöntemlerinin kullanılması ve Trikomoniyaz hastalarında miRNA profilinin belirlenmesi
Hasan Turgut, Fadime Eroğlu
Dicle Tıp Dergisi.2024; 51(1): 89. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Detection of Trichomonas vaginalis Infection in Chronic Prostatitis/Chronic Pelvic Pain Syndrome Patients by Rapid Immunochromatographic Test
Po-Chih Chang, Ming-Li Hsieh, Shih-Tsung Huang, Hsin-Chieh Huang, Yu-Chao Hsu, Ching-Wei Huang, Wei-Feng Ding, Yu Chen
Polish Journal of Microbiology.2022; 71(3): 301. CrossRef
Increased diagnostic yield of routine multiplex PCR compared to clinician requested testing for detection of Trichomonas vaginalis
Brooke Webb, Andrea Crampton, Michelle J. Francis, John Hamblin, Tony M. Korman, Maryza Graham
Pathology.2021; 53(2): 257. CrossRef
Signaling Role of Adipocyte Leptin in Prostate Cell Proliferation Induced by Trichomonas vaginalis
Jung-Hyun Kim, Ik-Hwan Han, Su-Jin Shin, Sung-Yul Park, Hyo-Yeoung Chung, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(3): 235. CrossRef
Cáncer y Microbiota
Francisco Arvelo, Felipe Sojo, Carlos Cotte
Investigación Clínica.2021; 62(4): 407. CrossRef
Proliferation of Mouse Prostate Cancer Cells Inflamed by Trichomonas vaginalis
Sang-Su Kim, Kyu-Shik Kim, Ik-Hwan Han, Yeseul Kim, Seong Sik Bang, Jung-Hyun Kim, Yong-Suk Kim, Soo-Yeon Choi, Jae-Sook Ryu
The Korean Journal of Parasitology.2021; 59(6): 547. CrossRef
IL-6 produced by prostate epithelial cells stimulated with Trichomonas vaginalis promotes proliferation of prostate cancer cells by inducing M2 polarization of THP-1-derived macrophages
Ik-Hwan Han, Hyun-Ouk Song, Jae-Sook Ryu, Michael H. Hsieh
PLOS Neglected Tropical Diseases.2020; 14(3): e0008126. CrossRef
Advancing Prevention of STIs by Developing Specific Serodiagnostic Targets: Trichomonas vginalis as a Model
John F. Alderete
International Journal of Environmental Research and Public Health.2020; 17(16): 5783. CrossRef
Experimental rat prostatitis caused by Trichomonas vaginalis infection
Ki‐Seok Jang, Ik‐Hwan Han, Seung‐Ju Lee, Jin Yoo, Ye‐Seul Kim, Seobo Sim, Jae‐Sook Ryu
The Prostate.2019; 79(4): 379. CrossRef
Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men
Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park
The Korean Journal of Parasitology.2019; 57(1): 21. CrossRef
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2019; 57(1): 27. CrossRef
Inflammatory mediators of prostate epithelial cells stimulated with Trichomonas vaginalis promote proliferative and invasive properties of prostate cancer cells
Ik‐Hwan Han, Jung‐Hyun Kim, Ki‐Seok Jang, Jae‐Sook Ryu
The Prostate.2019; 79(10): 1133. CrossRef
Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens
Collins Adjei, Richard Boateng, Albert Dompreh, Bismark Okyere, Eddie-Williams Owiredu
Tropical Medicine and Health.2019;[Epub] CrossRef
The Microbiome of Prostate Fluid Is Associated With Prostate Cancer
Xiaowei Ma, Chenfei Chi, Liancheng Fan, Baijun Dong, Xiaoguang Shao, Shaowei Xie, Min Li, Wei Xue
Frontiers in Microbiology.2019;[Epub] CrossRef
Proliferation of prostate epithelia induced by IL‐6 from stroma reacted with Trichomonas vaginalis
J.‐H. Kim, I.‐H. Han, Y.‐S. Kim, C.‐S. Noh, J.‐S. Ryu
Parasite Immunology.2018;[Epub] CrossRef
Trichomonas vaginalis infection and the diagnostic significance of detection tests among Ghanaian outpatients
Richard Harry Asmah, Rita Ofosuaa Agyeman, Noah Obeng-Nkrumah, Harriet Blankson, Georgina Awuah-Mensah, Momodou Cham, Listowell Asare, Patrick Ferdinand Ayeh-Kumi
BMC Women's Health.2018;[Epub] CrossRef
Epitopes within recombinant α-actinin protein is serodiagnostic target for Trichomonas vaginalis sexually transmitted infections
J.F. Alderete
Heliyon.2017; 3(1): e00237. CrossRef
Interaction between Trichomonas vaginalis and the Prostate Epithelium
Jung-Hyun Kim, Ik-Hwan Han, Sang-Su Kim, Soon-Jung Park, Duk-Young Min, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2017; 55(2): 213. CrossRef
Trichomonas vaginalis induces IL‐1β production in a human prostate epithelial cell line by activating the NLRP3 inflammasome via reactive oxygen species and potassium ion efflux
Na‐Yeong Gu, Jung‐Hyun Kim, Ik‐Hwan Han, Su‐Jeong Im, Min‐Young Seo, Yong‐Hoon Chung, Jae‐Sook Ryu
The Prostate.2016; 76(10): 885. CrossRef
Inflammatory response of a prostate stromal cell line induced by Trichomonas vaginalis
S. J. Im, I. H. Han, J. H. Kim, N. Y. Gu, M. Y. Seo, Y. H. Chung, J. S. Ryu
Parasite Immunology.2016; 38(4): 218. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis
Sang-Su Kim, Jung-Hyun Kim, Ik-Hwan Han, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 123. CrossRef
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated WithTrichomonas vaginalisvia Crosstalk With Mast Cell
Jung-Hyun Kim, Sang-Su Kim, Ik-Hwan Han, Seobo Sim, Myoung-Hee Ahn, Jae-Sook Ryu
The Prostate.2016; 76(15): 1431. CrossRef
Detection of Trichomonas vaginalis in prostate tissue and serostatus in patients with asymptomatic benign prostatic hyperplasia
Jamshaid Iqbal, Jumanah Al-Rashed, Elijah O. Kehinde
BMC Infectious Diseases.2016;[Epub] CrossRef
Signalling pathways associated with IL‐6 production and epithelial–mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis
I. H. Han, J. H. Kim, S. S. Kim, M. H. Ahn, J. S. Ryu
Parasite Immunology.2016; 38(11): 678. CrossRef
Epidemiology and laboratory characteristics ofTrichomonas vaginalisinfection in Croatian men with and without urethritis syndrome: a case–control study
Mario Sviben, Emilija Mlinarić Missoni, Tomislav Meštrović, Gordana Vojnović, Gordana Mlinarić Galinović
Sexually Transmitted Infections.2015; 91(5): 360. CrossRef
Trichomonas vaginalis: Clinical relevance, pathogenicity and diagnosis
Thomas Edwards, Patricia Burke, Helen Smalley, Glyn Hobbs
Critical Reviews in Microbiology.2014; : 1. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea
Jun-Hyeok Seo, Hye-Won Yang, So-Young Joo, Su-Min Song, Yu-Ran Lee, Jae-Sook Ryu, Eun Sang Yoo, Won Kee Lee, Hyun-Hee Kong, Sang-Eun Lee, Won-Ja Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2014; 52(5): 551. CrossRef
Inflammatory response of prostate epithelial cells to stimulation by Trichomonas vaginalis
Min‐Young Seo, Su‐Jeong Im, Na‐Young Gu, Jung‐Hyun Kim, Yong‐Hoon Chung, Myoung‐Hee Ahn, Jae‐Sook Ryu
The Prostate.2014; 74(4): 441. CrossRef
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
Hyun-Ouk Song, Jae-Sook Ryu
The Korean Journal of Parasitology.2013; 51(4): 479. CrossRef
A Survey Study of Trichomonas Vaginalis Infection in Unmarried Male Referred to Farmanfarmaean Clinic
Zarrintaj Valadkhani, Nayrreh Hassan, Zohreh Aghighi
Scimetr.2013;[Epub] CrossRef

15,361 View
130 Download
Crossref

NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products: Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin; Korean J Parasitol 2011;49(3):291-294.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.291

Abstract
PDF

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1×10⁷ live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-κB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with iκB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-κB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

Citations

Citations to this article as recorded by

Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis
Joshua A. Kochanowsky, Emma L. Betts, Gabriel Encinas, Johnson Amoah, Patricia J. Johnson
Proceedings of the National Academy of Sciences.2025;[Epub] CrossRef
CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 270. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation
Shao-Chun Wu, Pao-Jen Kuo, Cheng-Shyuan Rau, Lien-Hung Huang, Chia-Wei Lin, Yi-Chan Wu, Chia-Jung Wu, Chia-Wen Tsai, Ting-Min Hsieh, Hang-Tsung Liu, Chun-Ying Huang, Ching-Hua Hsieh
International Journal of Molecular Sciences.2021; 22(16): 8877. CrossRef
Trichomonas vaginalis‐secreted cysteinyl leukotrienes promote migration, degranulation and MCP‐1 production in mast cells
Young Ah Lee, Young Hee Nam, Arim Min, Myeong Heon Shin
Parasite Immunology.2020;[Epub] CrossRef
PKA/CREB and NF-κB pathway regulates AKNA transcription: A novel insight into T-2 toxin-induced inflammation and GH deficiency in GH3 cells
Xianglian Liu, Deyu Huang, Pu Guo, Qinghua Wu, Menghong Dai, Guyue Cheng, Haihong Hao, Shuyu Xie, Zonghui Yuan, Xu Wang
Toxicology.2017; 392: 81. CrossRef
SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete
Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton
Infection and Immunity.2017;[Epub] CrossRef
Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer
Juliana Garcia de Oliveira, Ana Flávia Teixeira Rossi, Daniela Manchini Nizato, Aline Cristina Targa Cadamuro, Yvana Cristina Jorge, Marina Curado Valsechi, Larissa Paola Rodrigues Venâncio, Paula Rahal, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo,
Tumor Biology.2015; 36(12): 9159. CrossRef
The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis
Wei-Chen Lin, Wei-Ting Chang, Tsuey-Yu Chang, Jyh-Wei Shin, Xuefeng Liu
PLOS ONE.2015; 10(4): e0124087. CrossRef

9,422 View
93 Download
Crossref

Identification of Antigenic Proteins in Trichomonas vaginalis: Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park; Korean J Parasitol 2011;49(1):79-83.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.79

Abstract
PDF

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage λ Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, α-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.

Citations

Citations to this article as recorded by

The Multifaceted Functions of Lactoferrin in Antimicrobial Defense and Inflammation
Jung Won Kim, Ji Seok Lee, Yu Jung Choi, Chaekyun Kim
Biomolecules.2025; 15(8): 1174. CrossRef
Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages
Emanuel Ceballos‐Góngora, Julio César Torres‐Romero, Victor Ermilo Arana‐Argáez, María Elizbeth Alvarez‐Sánchez, Karla Acosta‐Viana, Antonio Euan‐Canto, Leidi Cristal Alvarez‐Sánchez
Journal of Eukaryotic Microbiology.2024;[Epub] CrossRef
Activation of murine macrophages by membrane proteins from Tritrichomonas foetus grown on iron‐ and calcium‐rich conditions
Antonio Euan‐Canto, Julio César Torres‐Romero, María Elizbeth Alvarez‐Sánchez, Victor Ermilo Arana‐Argáez, Karla Acosta‐Viana, Emanuel Ceballos‐Góngora, Laura Vázquez‐Carrillo, Leidi Alvarez‐Sánchez
Parasite Immunology.2024;[Epub] CrossRef
Trichomonas vaginalis adhesion protein 65 (TvAP65) modulates parasite pathogenicity by interacting with host cell proteins
Zhenchao Zhang, Xiaoxiao Song, Yangyang Deng, Yuhua Li, Fakun Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Xuefang Mei, Shuai Wang
Acta Tropica.2023; 246: 106996. CrossRef
The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis
Zhenchao Zhang, Xiaoxiao Song, Zhengbo Zhang, Haoran Li, Yujuan Duan, Hao Zhang, Haoran Lu, Chengyang Luo, Mingyong Wang
Microbial Pathogenesis.2021; 152: 104750. CrossRef
Trichomonas vaginalis α-Actinin 2 Modulates Host Immune Responses by Inducing Tolerogenic Dendritic Cells via IL-10 Production from Regulatory T Cells
Hye-Yeon Lee, Juri Kim, Jae-Sook Ryu, Soon-Jung Park
The Korean Journal of Parasitology.2017; 55(4): 375. CrossRef
TvMP50 is an Immunogenic Metalloproteinase during Male Trichomoniasis
Laura Itzel Quintas-Granados, José Luis Villalpando, Laura Isabel Vázquez-Carrillo, Rossana Arroyo, Guillermo Mendoza-Hernández, María Elizbeth Álvarez-Sánchez
Molecular & Cellular Proteomics.2013; 12(7): 1953. CrossRef
Epitopes of the Highly Immunogenic Trichomonas vaginalis α-Actinin Are Serodiagnostic Targets for Both Women and Men
Calvin J. Neace, J. F. Alderete
Journal of Clinical Microbiology.2013; 51(8): 2483. CrossRef

10,767 View
155 Download
Crossref

Case Report

First Report of Feline Intestinal Trichomoniasis Caused by Tritrichomonas foetus in Korea: Sun Lim, Sang-Ik Park, Kyu-Sung Ahn, Dae-Sung Oh, Jae-Sook Ryu, Sung-Shik Shin; Korean J Parasitol 2010;48(3):247-251.
Published online September 16, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.3.247

Abstract
PDF

Feline intestinal tritrichomoniasis by Tritrichomonas foetus was first recognized in USA in 1999 and has so far been reported from UK, Norway, Switzerland, and Australia, but not from the Far East Asian countries. In November 2008, 2 female and male littermate Siamese cats, 6-month old, raised in a household in Korea were referred from a local veterinary clinic with a history of chronic persistent diarrhea. A direct smear examination of fecal specimens revealed numerous trichomonad trophozoites which were isolated by the fecal culture in InPouch™ TF-Feline medium. A PCR testing of the isolate based on the amplification of a conserved portion of the T. foetus internal transcribed spacer (ITS) regions (ITS1 and ITS2) and the 5.8S rRNA gene, and the molecular sequencing of the PCR amplicons confirmed infection with T. foetus. This is the first clinical case of feline intestinal trichomoniasis caused by T. foetus in Korea.

Citations

Citations to this article as recorded by

Prevalence of Tritrichomonas foetus Among Cats in Poland Between 2020 and 2024
Dawid Jańczak, Klaudiusz Szczepaniak, Jagoda Jeleniewska, Olga Szaluś-Jordanow
Pathogens.2025; 14(5): 458. CrossRef
Epidemiologic investigation of gastrointestinal pathogens for Korean cats with digestive sign
Mi-Jin Lee, Fujin An, Gijong Lee, Jin-ho Park
Korean Journal of Veterinary Service.2022; 45(2): 101. CrossRef
Prevalence of Tritrichomonas foetus infection in cats in Bangkok metropolitan area and in vitro drug sensitivity testing
Alisara Leelanupat, Ketsarin Kamyingkird, Wissanuwat Chimnoi, Burin Nimsuphan
Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100440. CrossRef
What is known about Tritrichomonas foetus infection in cats?
Bethânia Ferreira Bastos, Flavya Mendes de Almeida, Beatriz Brener
Revista Brasileira de Parasitologia Veterinária.2019; 28(1): 1. CrossRef
Prevalence of Cryptosporidium, Giardia, Blastocystis, and trichomonads in domestic cats in East China
Wenchao LI, Xinchao LIU, Youfang GU, Junlong LIU, Jianxun LUO
Journal of Veterinary Medical Science.2019; 81(6): 890. CrossRef
Development and comparative evaluation of different LAMP and PCR assays for coprological diagnosis of feline tritrichomonosis
Joanna Dąbrowska, Jacek Karamon, Maciej Kochanowski, Bruno Gottstein, Tomasz Cencek, Caroline F. Frey, Norbert Müller
Veterinary Parasitology.2019; 273: 17. CrossRef
Detection of natural occurrence of Tritrichomonas foetus in cats in Araçatuba, São Paulo, Brazil
Roberta P. Duarte, Paulo Ricardo D.A. Rocha, Alex A. Nakamura, Rafael S. Cipriano, Milena A. Viol, Guilherme D. Melo, Marcelo V. Meireles, Gisele F. Machado
Pesquisa Veterinária Brasileira.2018; 38(2): 309. CrossRef
Türkiye’de Kronik İshalli Kedilerde Tritrichomonas foetus’ün Araştırılması ve Risk Faktörlerinin Belirlenmesi
Didem PEKMEZCİ, Gökmen Zafer PEKMEZCİ, Ümit ÖZCAN, Duygu DALGIN, Mehmet TÜTÜNCÜ
Etlik Veteriner Mikrobiyoloji Dergisi.2018; 29(2): 116. CrossRef
Efficacy of guar gum-based ronidazole capsules as a treatment for Tritrichomonas foetus infection in cats
Aurélien Grellet, Seyf Eddine Makhlouf, Loic Desquilbet, Fani Hovhannessian, Cassandre Boogaerts, Vanessa Dore, Myriam Anthony, Bernadette Espana, Caroline Prouillac, Plamen Kirilov, Bruno Polack, Sébastien Perrot
Journal of Feline Medicine and Surgery.2017; 19(2): 177. CrossRef
Comparative proteomic analysis of two pathogenic Tritrichomonas foetus genotypes: there is more to the proteome than meets the eye
Leah J. Stroud, Jan Šlapeta, Matthew P. Padula, Dylan Druery, George Tsiotsioras, Jens R. Coorssen, Colin M. Stack
International Journal for Parasitology.2017; 47(4): 203. CrossRef
The conundrum of feline trichomonosis: the more we learn the ‘trickier’ it gets
Jody L Gookin, Katherine Hanrahan, Michael G Levy
Journal of Feline Medicine and Surgery.2017; 19(3): 261. CrossRef
Cross-sectional survey on Tritrichomonas foetus infection in Italian cats
F. Veronesi, A.L. Gazzonis, E. Napoli, E. Brianti, A. Santoro, S.A. Zanzani, E. Olivieri, M. Diaferia, S. Giannetto, M.G. Pennisi, M.T. Manfredi
Veterinary Parasitology: Regional Studies and Reports.2016; 6: 14. CrossRef
Tritrichomonas foetus infection, a cause of chronic diarrhea in the domestic cat
Chaoqun Yao, Liza S Köster
Veterinary Research.2015;[Epub] CrossRef
Comparative RNA-seq analysis of the Tritrichomonas foetus PIG30/1 isolate from pigs reveals close association with Tritrichomonas foetus BP-4 isolate ‘bovine genotype’
Victoria Morin-Adeline, Kai Mueller, Ana Conesa, Jan Šlapeta
Veterinary Parasitology.2015; 212(3-4): 111. CrossRef
High prevalence of Tritrichomonas foetus ‘bovine genotype’ in faecal samples from domestic pigs at a farm where bovine trichomonosis has not been reported for over 30 years
Kai Mueller, Victoria Morin-Adeline, Katrina Gilchrist, Graeme Brown, Jan Šlapeta
Veterinary Parasitology.2015; 212(3-4): 105. CrossRef
Tritrichomonas foetus infection in cat – first detection in Poland
Joanna Dąbrowska, Jacek Karamon, Maciej Kochanowski, Roman Jędryczko, Tomasz Cencek
Acta Parasitologica.2015;[Epub] CrossRef
Functional profiling of the Tritrichomonas foetus transcriptome and proteome
Kuo-Yang Huang, Jyh-Wei Shin, Po-Jung Huang, Fu-Man Ku, Wei-Chen Lin, Rose Lin, Wei-Min Hsu, Petrus Tang
Molecular and Biochemical Parasitology.2013; 187(1): 60. CrossRef
Isolation of Tritrichomonas foetus from cats sampled at a cat clinic, cat shows and a humane society in southern Ontario
Ansarah Hosein, Stephen A Kruth, David L Pearl, Danielle Richardson, Jocelyn C Maggs, Hillary A Peach, Andrew S Peregrine
Journal of Feline Medicine and Surgery.2013; 15(8): 706. CrossRef
Molecular Survey of Tritrichomonas suis (=T. foetus) ‘Cat’ and ‘Cattle’ Genotypes in Pigs in Japan
Junko DOI, Niichiro ABE, Yuzaburo OKU
Journal of Veterinary Medical Science.2013; 75(4): 475. CrossRef
Tritrichomonas – Systematics of an enigmatic genus
Caroline F. Frey, Norbert Müller
Molecular and Cellular Probes.2012; 26(3): 132. CrossRef
Efficacy of Ronidazole for Treatment of Cats Experimentally Infected with a Korean Isolate of Tritrichomonas foetus
Sun Lim, Sang-Ik Park, Kyu-Sung Ahn, Dae-Sung Oh, Sung-Shik Shin
The Korean Journal of Parasitology.2012; 50(2): 161. CrossRef
Detection of Tritrichomonas foetus and Pentatrichomonas hominis in intestinal tissue specimens of cats by chromogenic in situ hybridization
Meike M. Mostegl, Andreas Wetscher, Barbara Richter, Nora Nedorost, Nora Dinhopl, Herbert Weissenböck
Veterinary Parasitology.2012; 183(3-4): 209. CrossRef

14,456 View
190 Download
Crossref

Original Articles

Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate: Hyun-Ouk Song, Young-Su Lim, Sun-Joo Moon, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2010;48(1):1-7.
Published online March 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.1.1

Abstract
PDF

Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.

Citations

Citations to this article as recorded by

The role of neutrophils in the pathogenesis of Trichomonas vaginalis infection in pregnant women: A review
Emmanuel Ifeanyi Obeagu, Gideon Ikechukwu Okoroiwu, Nwanganga Ihuoma Ubosi, Getrude Uzoma Obeagu, Ebere Emilia Ayogu, Elham Elamin
Medicine.2025; 104(41): e45063. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth
María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila
Parasitology Research.2022; 121(5): 1355. CrossRef
Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis
F. J. Rangel-Mata, E. E. Ávila-Muro, J. E. Reyes-Martínez, L. M. Olmos-Ortiz, M. E. Brunck, L. A. Arriaga-Pizano, P. Cuéllar-Mata
Parasitology.2021; 148(13): 1624. CrossRef
Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells
Fei Fei Gao, Juan-Hua Quan, Min A. Lee, Wei Ye, Jae-Min Yuk, Guang-Ho Cha, In-Wook Choi, Young-Ha Lee
Parasites & Vectors.2021;[Epub] CrossRef
Molecular investigation of Trichomoniasis in women in Al-Muthana province /Iraq
H R J Al-abodi, K T M Al-Shaibani, E M Shaker
Journal of Physics: Conference Series.2019; 1234(1): 012078. CrossRef
Amaurocine: Anti- Trichomonas vaginalis protein produced by the basidiomycete Amauroderma camerarium
Mariana Duarte, Adriana Seixas, Maira Peres de Carvalho, Tiana Tasca, Alexandre José Macedo
Experimental Parasitology.2016; 161: 6. CrossRef
Trichomonas vaginalis induces cytopathic effect on human lung alveolar basal carcinoma epithelial cell line A549
Daile Meek C. Salvador-Membreve, Sonia D. Jacinto, Windell L. Rivera
Experimental Parasitology.2014; 147: 33. CrossRef
Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes
Juan-Hua Quan, Byung-Hun Kang, Guang-Ho Cha, Wei Zhou, Young-Bok Koh, Jung-Bo Yang, Heon-Jong Yoo, Min-A Lee, Jae-Sook Ryu, Heung-Tae Noh, Jaeyul Kwon, Young-Ha Lee, A. R. M. Ruhul Amin
PLoS ONE.2014; 9(10): e110659. CrossRef
Altered CD16 expression on vaginal neutrophils from women with vaginitis
Joziani Beghini, Paulo C. Giraldo, Ruth Riboldi, Rose L.G. Amaral, José Eleutério, Steven S. Witkin, Fernando Guimarães
European Journal of Obstetrics & Gynecology and Reproductive Biology.2013; 167(1): 96. CrossRef
Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products
Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin
Inflammation Research.2012; 61(2): 97. CrossRef
NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced byTrichomonas vaginalis-Derived Secretory Products
Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2011; 49(3): 291. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef

10,082 View
94 Download
Crossref

Intestinal Parasitic Infections among Inhabitants of Karaj City, Tehran Province, Iran in 2006-2008: Vahid Nasiri, Kasra Esmailnia, Gholamreza Karim, Mehdi Nasir, Omid Akhavan; Korean J Parasitol 2009;47(3):265-268.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.265

Abstract
PDF

Karaj is an area with large influx of refugee people in Iran. To increase knowledge about parasitic infections, we carried out this research during 2006-2008. We recorded the stool examination results and some of their personal characteristics. A total of 13,915 human stools were examined, and 649 (4.7%) were positive for intestinal parasites. Among them, 13 (0.09%) had worm and 636 (4.6%) had protozoan infections. Maximum infections belonged to Giardia intestinalis, and 534 (3.8%) samples had this infection. Other parasitic infections included Entamoeba coli (0.39%), Entamoeba histolytica (0.021%), Blastocystis hominis (0.08%), Trichomonas hominis (0.1%), Iodamoeba butschlii (0.06%), Chilomastix mesnili (0.007%), Endolimax nana (0.05%), Enterobius spp. eggs (0.028%), Taenia proglottids (0.028%), and Strongyloides stercoralis larvae (0.03%). The maximum numbers of referred people to laboratories were in July and the maximum percentage of infections was in August. There is a point that all 5 Strongyloides stercoralis infections were pertained to 2008. With attention to the rate of parasitic infections (4.7%), it seems that we should take additional educational information to wide spectrum of people living in this city.

Citations

Citations to this article as recorded by

Effectiveness of Anthelmintic Therapy and Determinants of Ascaris lumbricoides Infection among School-Aged Children: A Community-Based Cross-Sectional Study in Rural Khyber Pakhtunkhwa, Pakistan
Amjad Ullah Khan, Shahzad Hussain, Majid Khan, Ali Said, Aqsa Bibi, Talha Khan, Sabika Firasat, Umer Chaudhry, Kiran Afshan
Acta Parasitologica.2025;[Epub] CrossRef
Prevalence of Intestinal Protozoa Infections Among Some Primary School Pupils in Akko Local Government Area of Gombe State, Nigeria
M. S. Twan, Oguche A. J.
African Journal of Biology and Medical Research.2024; 7(3): 110. CrossRef
Occurrence and assemblage distribution of Giardia Duodenalis in symptomatic and asymptomatic patients in southeastern Iran (2019–2022)
Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Mostafa Rezaeian, Hanieh Mohammad Rahimi, Ahmadreza Meamar, Milad Badri, Aida Vafae Eslahi, Hossein-Ali Rahdar, Solmaz Sepahi, Hamed Mirjalali, David Carmena
Gut Pathogens.2024;[Epub] CrossRef
Prevalence and subtyping of Blastocystis sp. in ruminants in Southwestern, Iran
Mahboubeh Heydarian, Kourosh Manouchehri Naeini, Soleiman Kheiri, Rahman Abdizadeh
Scientific Reports.2024;[Epub] CrossRef
Intestinal Protozoa and Schistosoma Mansoni (Sambon 1907) Infections Among Some Pupils in Akko Local Government Area of Gombe State, Nigeria
M. S. Twan
African Journal of Biology and Medical Research.2024; 7(3): 119. CrossRef
The Effect of the COVID-19 Pandemic Process on the Incidence of Intestinal Parasites; Aydın Province Example
İbrahim Yıldız, Evren Tileklioğlu
Turkish Journal of Parasitology.2023; 47(2): 100. CrossRef
Prevalence of Intestinal Parasitic Infections and Associated Risk Factors Among in Children Ilam Town, West Iran
Soghra Viesy, Amin Jaydari, Maryam Hataminejad, Marzieh Shadpirouz, Iman Pouladi
Pediatric Infection & Vaccine.2023; 30(3): 145. CrossRef
Intestinal parasitic infections among patients referred to hospitals affiliated to Shiraz University of Medical Sciences, southern Iran: a retrospective study in pre- and post-COVID-19 pandemic
Aref Teimouri, Rasoul Alimi, Samaneh Farsi, Fattaneh Mikaeili
Environmental Science and Pollution Research.2022; 29(24): 36911. CrossRef
Prevalence and Associated Risk Factors of Human Intestinal Helminths Parasitic Infections in Ethiopia: A Systematic Review and Meta-Analysis
Minichil Liyih, Destaw Damtie, Dires Tegen, Ahmad Mansour
The Scientific World Journal.2022; 2022: 1. CrossRef
Prevalence and Risk Factors Associated with Intestinal Parasitic Infection among Primary School Children in Dera District, Northwest Ethiopia
Dires Tegen, Destaw Damtie, Julia Uhanova
Canadian Journal of Infectious Diseases and Medical Microbiology.2021; 2021: 1. CrossRef
Prevalence and risk factors of intestinal parasite infections in Mazandaran province, North of Iran
Zeinab Gholipoor, Hooshang Khazan, Eznoallah Azargashb, Mohammad Reza Youssefi, Ali Rostami
Clinical Epidemiology and Global Health.2020; 8(1): 17. CrossRef
Prevalence and associated risk factors of human intestinal parasitic infections: a population-based study in the southeast of Kerman province, southeastern Iran
Mohammad Javad Abbaszadeh Afshar, Maryam Barkhori Mehni, Mostafa Rezaeian, Mehdi Mohebali, Vali Baigi, Somayeh Amiri, Mohammad Bagher Amirshekari, Ruhollah Hamidinia, Mohammad Samimi
BMC Infectious Diseases.2020;[Epub] CrossRef
The Frequency of the Intestinal Parasites Giardia Lamblia and Entamoeba Histolytica in Pediatric Diarrhea Specimens from Central Iran
Elnaz Abbasi, Alireza Amouzandeh-Nobaveh, Ehsanollah Ghaznavi-Rad
The Open Microbiology Journal.2020; 14(1): 53. CrossRef
Prevalence and Associated Risk Factors of Human Intestinal Protozoan Parasitic Infections in Ethiopia: A Systematic Review and Meta-Analysis
Dires Tegen, Destaw Damtie, Tamirat Hailegebriel
Journal of Parasitology Research.2020; 2020: 1. CrossRef
Epidemiology of taeniosis, cysticercosis and trichinellosis in Iran: A systematic review
Mohammad Moazeni, Faham Khamesipour, Douglas N. Anyona, Gabriel O. Dida
Zoonoses and Public Health.2019; 66(1): 140. CrossRef
Giardia lamblia assemblages A and B isolated from symptomatic and asymptomatic persons in Hamadan, west of Iran
Manizheh Kashinahanji, Ali Haghighi, Fares Bahrami, Mohammad Fallah, Masoud Saidijam, Mohammad Matini, Amir Hossein Maghsood
Journal of Parasitic Diseases.2019; 43(4): 616. CrossRef
Fatty acid and retinol-binding protein: A novel antigen for immunodiagnosis of human strongyloidiasis
Leila Masoori, Ahmad Reza Meamar, Mojgan Bandehpour, Andrew Hemphill, Elham Razmjou, Kobra Mokhtarian, Mona Roozbehani, Alireza Badirzadeh, Nahid Jalallou, Lame Akhlaghi, Reza Falak, Chia Kwung Fan
PLOS ONE.2019; 14(7): e0218895. CrossRef
Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes
Carolina Valença Barbosa, Magali Muniz Barreto, Rosemary de Jesus Andrade, Fernando Sodré, Claudia Masini d’Avila-Levy, José Mauro Peralta, Ricardo Pereira Igreja, Heloisa Werneck de Macedo, Helena Lucia Carneiro Santos, Maria Victoria Periago
PLOS ONE.2018; 13(3): e0193860. CrossRef
Rodent-borne diseases and their public health importance in Iran
Mohammad Hasan Rabiee, Ahmad Mahmoudi, Roohollah Siahsarvie, Boris Kryštufek, Ehsan Mostafavi, Peter J. Krause
PLOS Neglected Tropical Diseases.2018; 12(4): e0006256. CrossRef
Impacts of human development index and climate conditions on prevalence of Blastocystis: A systematic review and meta-analysis
Ehsan Javanmard, Maryam Niyyati, Erfan Ghasemi, Hamed Mirjalali, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Acta Tropica.2018; 185: 193. CrossRef
Amoebiasis in Iran: a systematic review and meta-analysis
Ali Haghighi, Seyed Mohammad Riahi, Ali Taghipour, Adel Spotin, Mostafa Javanian, Mohsen Mohammadi, Mohammadreza Esmaeili Dooki, Ali Rostami
Epidemiology and Infection.2018; 146(15): 1880. CrossRef
Prevalence and associated risk factors of intestinal parasitic infections in Kurdistan province, northwest Iran
Fares Bahrami, Ali Haghighi, Ghasem Zamini, Mohammad Bagher Khadem-Erfan, Eznolla Azargashb, Andrew Kiyu
Cogent Medicine.2018; 5(1): 1503777. CrossRef
Prevalence of Intestinal Parasitic Infections among Patients Referring to Medical Diagnostic Laboratories, Kashan, Central Iran, 2015-2018
Shirin Khodabakhsh-Arbat, Hossein Hooshyar, Hadi Sadeghi
Journal of Medical Microbiology and Infectious Diseases .2018; 6(2): 62. CrossRef
Bacterial assessment of food handlers in Sari City, Mazandaran Province, north of Iran
Mohtaram Nasrolahei, Siavash Mirshafiee, Soudeh Kholdi, Maryam Salehian, Masoumeh Nasrolahei
Journal of Infection and Public Health.2017; 10(2): 171. CrossRef
Intestinal parasitic infections in different groups of immunocompromised patients in Kashan and Qom cities, central Iran
Sima Rasti, Malihe Hassanzadeh, Hossein Hooshyar, Mansooreh Momen-Heravi, Seyed Gholam Abbas Mousavi, Amir Abdoli
Scandinavian Journal of Gastroenterology.2017; 52(6-7): 738. CrossRef
Prevalence of Intestinal Parasitic Infections in Shush County, Southwest of Iran during 2014 - 2016
Somayeh Fallahizadeh, Mohammad Hossein Feiz-Haddad, Forough Kazemi, Reza Afrisham
International Journal of Infection.2017;[Epub] CrossRef
Prevalence of Intestinal Parasite Infections among Children in the Day Care Centers of Gonbad-e Kavus County, North-Eastern Iran
Fatemeh Mesgarian, Aioub Sofizadeh, Hamid Reza Shoraka, Hamid Reza Rahimi, Ali Hesari, Najmeddin Soheili, Farhad Badiei
Zahedan Journal of Research in Medical Sciences.2017;[Epub] CrossRef
Intestinal parasitic infections among expatriate workers in various occupations in Sharjah, United Arab Emirates
Abdelmunim Izzeldin Abdelrahman Dafalla, Shaikha Ali Salem Obaid Almuhairi, Mohamed Hassan Jasim AlHosani, Mira Yousif Mohamed, Mariam Ibrahim Ahmed Alkous, Mousa Abdelsattar AlAzzawi, Adam Dawoud Abakar, Bakri Yousif Mohamed Nour, Hayder Hasan, Ra'ed Oma
Revista do Instituto de Medicina Tropical de São Paulo.2017;[Epub] CrossRef
A Survey on Prevalence of Intestinal Parasites Infections in Patients Referred to the Public Hospital in Khoy, West Azarbaijan Province, Iran, 2014 - 2016
Ebrahim Kazemi, Parvin Rostamkhani, Hossein Hooshyar
Avicenna Journal of Clinical Microbiology and Infection.2017; 4(4): 56114. CrossRef
Prevalence of Enterobius vermicularis amongst kindergartens and preschool children in Mazandaran Province, North of Iran
Narges Afrakhteh, Zahra Marhaba, Seif Ali Mahdavi, Sahar Garoosian, Reyhaneh Mirnezhad, Mahsa Eshkevar Vakili, Haniye Ahmadi Shahraj, Behzad Javadian, Rozita Rezaei, Mahmood Moosazadeh
Journal of Parasitic Diseases.2016; 40(4): 1332. CrossRef
RETRACTED ARTICLE: Evaluation of intestinal parasites among referred patients: report of 4637 cases
Ali Asgari, Mohammad Davood Sharifi, Hosein Amini Anabad, Amin Zamani Nughabi, Amir Farshid Fayyaz, Ali Akbar Jafari
Journal of Parasitic Diseases.2016; 40(4): 1640. CrossRef
RETRACTED ARTICLE: Frequency of intestinal protozoan parasites in patients with gastrointestinal disorders in south of Karaj, Iran
Mohammad Sabzikarian, Saeideh Najafi, Hosein Amini Anabad, Vahid Behnod, Emad Yahaghi, Koorosh Ahmadi
Journal of Parasitic Diseases.2016; 40(4): 1641. CrossRef
Contribution of environmental media to cryptosporidiosis and giardiasis prevalence in Tehran: a focus on surface waters
Mahdi Hadi, Alireza Mesdaghinia, Masud Yunesian, Simin Nasseri, Ramin Nabizadeh Nodehi, Hamidreza Tashauoei, Esfandiar Jalilzadeh, Roya Zarinnejad
Environmental Science and Pollution Research.2016; 23(19): 19317. CrossRef
Prevalence and Genotype Analysis of Blastocystis hominis in Iran: A Systematic Review and Meta-Analysis
Ebrahim Badparva, Behrouz Ezatpour, Hossein Mahmoudvand, Masoud Behzadifar, Meysam Behzadifar, Farnaz Kheirandish
Archives of Clinical Infectious Diseases.2016;[Epub] CrossRef
Zoonotic intestinal protozoan of the wild boars, Sus scrofa, in Persian Gulf’s coastal area (Bushehr province), Southwestern Iran
Kambiz Yaghoobi, Bahador Sarkari, Majid Mansouri, Mohammad Hossein Motazedian
Veterinary World.2016; 9(10): 1047. CrossRef
Prevalence and risk factors of intestinal protozoan infections: a population-based study in rural areas of Boyer-Ahmad district, Southwestern Iran
Bahador Sarkari, Ghasem Hosseini, Mohammad Hossein Motazedian, Mohammad Fararouei, Abdolali Moshfe
BMC Infectious Diseases.2016;[Epub] CrossRef
PREVALENCE, RISK FACTORS AND SYMPTOMS ASSOCIATED TO INTESTINAL PARASITE INFECTIONS AMONG PATIENTS WITH GASTROINTESTINAL DISORDERS IN NAHAVAND, WESTERN IRAN
Hamed KIANI, Ali HAGHIGHI, Ali ROSTAMI, Eznollah AZARGASHB, Seyyed Javad Seyyed TABAEI, Abbas SOLGI, Nozhat ZEBARDAST
Revista do Instituto de Medicina Tropical de São Paulo.2016;[Epub] CrossRef
Prevalence of Intestinal Parasitic Infections in Haftkel County, Southwest of Iran
Jasem Saki, Shahram Khademvatan, Masoud Foroutan-Rad, Marziyeh Gharibzadeh
International Journal of Infection.2016;[Epub] CrossRef
Prevalence of intestinal parasites in a population in Eghbalieh city from Qazvin Province, Iran
H. Sadeghi, M. Bakht, H. Saghafi, T. Shahsavari
Journal of Parasitic Diseases.2015; 39(2): 126. CrossRef
Prevalence of Intestinal Parasitic Infections Among People in Baghmalek During 2013 ‒ 2014
Saleh Khoshnood, Jasem Saki, Abdollah Rafiei, Kobra Alizadeh
Jentashapir Journal of Health Research.2015;[Epub] CrossRef
Prevalence of Intestinal Parasitic Infections Among People in Baghmalek During 2013 ‒ 2014
Saleh Khoshnood, Jasem Saki, Abdollah Rafiei, Kobra Alizadeh
Jentashapir Journal of Health Research.2015;[Epub] CrossRef
Prevalence of Intestinal Parasitic Infections Among People in Baghmalek During 2013 ‒ 2014
Saleh Khoshnood, Jasem Saki, Abdollah Rafiei, Kobra Alizadeh
Jentashapir Journal of Health Research.2015;[Epub] CrossRef
PREVALENCE OF INTESTINAL PARASITES AMONG FOOD HANDLERS OF SARI, NORTHERN IRAN
Mehdi SHARIF, Ahmad DARYANI, Elham KIA, Fateme REZAEI, Mehrdad NASIRI, Mohtaram NASROLAHEI
Revista do Instituto de Medicina Tropical de São Paulo.2015; 57(2): 139. CrossRef
Soil-Transmitted Helminth Infections and Associated Risk Factors in Three Orang Asli Tribes in Peninsular Malaysia
Tengku Shahrul Anuar, Fatmah Md Salleh, Norhayati Moktar
Scientific Reports.2014;[Epub] CrossRef
Prevalence of intestinal parasites in Lorestan Province, West of Iran
Ebrahim Badparva, Farnaz Kheirandish, Farzad Ebrahimzade
Asian Pacific Journal of Tropical Disease.2014; 4: S728. CrossRef
Prevalence of Intestinal Parasitic Infections Among Rural Inhabitants of Hamadan City, Iran, 2012
Rasool Jafari, Mohammad Fallah, Hossein Yousofi Darani, Hosein Ali Yousefi, Mohammad Ali Mohaghegh, Milad Latifi, Mohammad Sadaghian, Amir Hossein Maghsood
Avicenna Journal of Clinical Microbiology and Infection.2014; 1(2): 21445. CrossRef
Strongyloidiasis—An Insight into Its Global Prevalence and Management
Santhosh Puthiyakunnon, Swapna Boddu, Yiji Li, Xiaohong Zhou, Chunmei Wang, Juan Li, Xiaoguang Chen, Gary L. Simon
PLoS Neglected Tropical Diseases.2014; 8(8): e3018. CrossRef
PREVALENCE OF INTESTINAL PARASITES AMONG FOOD HANDLERS IN WESTERN IRAN
Farnaz Kheirandish, Mohammad Javad Tarahi, Behrouz Ezatpour
Revista do Instituto de Medicina Tropical de São Paulo.2014; 56(2): 111. CrossRef
Food and drinking water hygiene and intestinal protozoa in deployed German soldiers
Hagen Frickmann, Norbert G. Schwarz, Dorothea F. Wiemer, Marcellus Fischer, Egbert Tannich, Patrick L. Scheid, Martin Müller, Ulrich Schotte, Wolfgang Bock, Ralf M. Hagen
European Journal of Microbiology and Immunology.2013; 3(1): 53. CrossRef
Prevalence of Strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran
Azar Shokri, Khojasteh Sharifi Sarasiabi, Saeed Hosseini Teshnizi, Hamid Mahmoodi
Asian Pacific Journal of Tropical Biomedicine.2012; 2(2): 88. CrossRef
Intestinal Parasitic Infection among School Children in Golestan Province, Iran
Rostami Masoumeh, Tohidi Farideh, Sharbatkhori Mitra, Taherkhani Heshmatoll
Pakistan Journal of Biological Sciences.2012; 15(23): 1119. CrossRef
Epidemiological survey of the prevalence of intestinal parasites among schoolchildren in Sari, northern Iran
A. Daryani, M. Sharif, M. Nasrolahei, A. Khalilian, A. Mohammadi, Gh. Barzegar
Transactions of the Royal Society of Tropical Medicine and Hygiene.2012; 106(8): 455. CrossRef
A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran
Khosro Sardarian, Mehrdad Hajilooi, Amirhosein Maghsood, Abbas Moghimbeigi, Mohamadyusef Alikhani
Jundishapur Journal of Microbiology.2012; 5(4): 555. CrossRef
A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran
Khosro Sardarian, Mehrdad Hajilooi, Amirhosein Maghsood, Abbas Moghimbeigi, Mohamadyusef Alikhani
Jundishapur Journal of Microbiology.2012; 6(1): 11. CrossRef
Prevalence of Parasitic Contamination of Raw Vegetables in Villages of Qazvin Province, Iran
Mojtaba Shahnazi, Majid Jafari-Sabet
Foodborne Pathogens and Disease.2010; 7(9): 1025. CrossRef
Intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern Iran
Mehdi Sharif, Ahmad Daryani, Fatemeh Asgarian, Mohtaram Nasrolahei
Research in Developmental Disabilities.2010; 31(4): 924. CrossRef

10,760 View
142 Download
Crossref

Proinflammatory Cytokine and Nitric Oxide Production by Human Macrophages Stimulated with Trichomonas vaginalis: Ik-Hwan Han, Sung Young Goo, Soon-Jung Park, Se-Jin Hwang, Yong-Seok Kim, Michael Sungwoo Yang, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2009;47(3):205-212.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.205

Abstract
PDF

Trichomonas vaginalis commonly causes vaginitis and perhaps cervicitis in women and urethritis in men and women. Macrophages are important immune cells in response to T. vaginalis infection. In this study, we investigated whether human macrophages could be involved in inflammation induced by T. vaginalis. Human monocyte-derived macrophages (HMDM) were co-cultured with T. vaginalis. Live, opsonized-live trichomonads, and T. vaginalis lysates increased proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6 by HMDM. The involvement of nuclear factor (NF)-κB signaling pathway in cytokine production induced by T. vaginalis was confirmed by phosphorylation and nuclear translocation of p65 NF-κB. In addition, stimulation with live T. vaginalis induced marked augmentation of nitric oxide (NO) production and expression of inducible NO synthase (iNOS) levels in HMDM. However, trichomonad-induced NF-κB activation and TNF-α production in macrophages were significantly inhibited by inhibition of iNOS levels with L-NMMA (NO synthase inhibitor). Moreover, pretreatment with NF-κB inhibitors (PDTC or Bay11-7082) caused human macrophages to produce less TNF-α. These results suggest that T. vaginalis stimulates human macrophages to produce proinflammatory cytokines, such as IL-1, IL-6, and TNF-α, and NO. In particular, we showed that T. vaginalis induced TNF-α production in macrophages through NO-dependent activation of NF-κB, which might be closely involved in inflammation caused by T. vaginalis.

Citations

Citations to this article as recorded by

Impact of microparticles released during murine systemic inflammation on macrophage activity and reactive nitrogen species regulation
Weronika Ortmann, Anna Such, Elzbieta Kolaczkowska
Immunologic Research.2024; 72(2): 299. CrossRef
Activation of murine macrophages by membrane proteins from Tritrichomonas foetus grown on iron‐ and calcium‐rich conditions
Antonio Euan‐Canto, Julio César Torres‐Romero, María Elizbeth Alvarez‐Sánchez, Victor Ermilo Arana‐Argáez, Karla Acosta‐Viana, Emanuel Ceballos‐Góngora, Laura Vázquez‐Carrillo, Leidi Alvarez‐Sánchez
Parasite Immunology.2024;[Epub] CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
In Vitro Activation of Macrophages by an MHC Class II-restricted Trichomonas Vaginalis TvZIP8-derived Synthetic Peptide
Victor Ermilo Arana-Argáez, Emanuel Ceballos-Góngora, María Elizbeth Alvarez-Sánchez, Antonio Euan-Canto, Julio Lara-Riegos, Julio César Torres-Romero
Immunological Investigations.2022; 51(1): 88. CrossRef
Local cytokine/chemokine profiles in BALB/c and C57BL/6 mice in response to T. vaginalis infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Experimental Parasitology.2022; 239: 108287. CrossRef
Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis
F. J. Rangel-Mata, E. E. Ávila-Muro, J. E. Reyes-Martínez, L. M. Olmos-Ortiz, M. E. Brunck, L. A. Arriaga-Pizano, P. Cuéllar-Mata
Parasitology.2021; 148(13): 1624. CrossRef
Trichomonas vaginalis triggers the release of THP-1 extracellular traps
Li Fei, Wei Zhengkai, Jiang Weina, Cao Lili, Gao Yuhang, Yang Zhengtao, Li Jianhua, Yu Biao, Zhang Xichen, Gong Pengtao
Parasitology Research.2019; 118(1): 267. CrossRef
Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis
Tahali Mendoza-Oliveros, Victor Arana-Argáez, Leidi C. Alvaréz-Sánchez, Julio Lara-Riegos, María Elizbeth Alvaréz-Sánchez, Julio C. Torres-Romero
The Korean Journal of Parasitology.2019; 57(1): 33. CrossRef
Heterogeneous macrophages: Supersensors of exogenous inducing factors
Caiyun Qian, Zehui Yun, Yudi Yao, Minghua Cao, Qiang Liu, Song Hu, Shuhua Zhang, Daya Luo
Scandinavian Journal of Immunology.2019;[Epub] CrossRef
Immunopathology of Recurrent Vulvovaginal Infections: New Aspects and Research Directions
Namarta Kalia, Jatinder Singh, Manpreet Kaur
Frontiers in Immunology.2019;[Epub] CrossRef
ReducedTrichomonas vaginalisviability in mice pretreated with parasite DNA
Marco Antonio Barajas-Mendiola, Laura E. Castellano, Miriam Barrios-Rodiles, Martha A. Deveze-Alvarez, Eva E. Avila, Patricia Cuéllar-Mata
Parasitology.2019; 146(13): 1636. CrossRef
Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages
Angelica Montenegro Riestra, J. Andrés Valderrama, Kathryn A. Patras, Sharon D. Booth, Xing Yen Quek, Chih-Ming Tsai, Victor Nizet
Journal of Innate Immunity.2019; 11(1): 86. CrossRef
Humoral and T cell–mediated immune response against trichomoniasis
M. Nemati, N. Malla, M. Yadav, H. Khorramdelazad, A. Jafarzadeh
Parasite Immunology.2018;[Epub] CrossRef
Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-κB Pathways Partially Mediated by TLR2
Ling Li, Xin Li, Pengtao Gong, Xichen Zhang, Zhengtao Yang, Ju Yang, Jianhua Li
Frontiers in Microbiology.2018;[Epub] CrossRef
BLT1-mediated O-GlcNAcylation is required for NOX2-dependent migration, exocytotic degranulation and IL-8 release of human mast cell induced by Trichomonas vaginalis-secreted LTB4
Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon Shin
Microbes and Infection.2018; 20(6): 376. CrossRef
Effect of iNOS inhibitor LNMMA along with antibiotics Chloramphenicol or Ofloxacin in murine peritoneal macrophages regulates S.aureus infection as well as inflammation: An in vitro study
Somrita Dey, Biswadev Bishayi
Microbial Pathogenesis.2017; 105: 307. CrossRef
Trichomonas vaginalis exosome‐like vesicles modify the cytokine profile and reduce inflammation in parasite‐infected mice
L. M. Olmos‐Ortiz, M. A. Barajas‐Mendiola, M. Barrios‐Rodiles, L. E. Castellano, S. Arias‐Negrete, E. E. Avila, P. Cuéllar‐Mata
Parasite Immunology.2017;[Epub] CrossRef
The anti‐Trichomonas vaginalis phloroglucinol derivative isoaustrobrasilol B modulates extracellular nucleotide hydrolysis
Camila Braz Menezes, Graziela Vargas Rigo, Henrique Bridi, Danielle da Silva Trentin, Alexandre José Macedo, Gilsane Lino von Poser, Tiana Tasca
Chemical Biology & Drug Design.2017; 90(5): 811. CrossRef
Adenosine reduces reactive oxygen species and interleukin-8 production by Trichomonas vaginalis-stimulated neutrophils
Amanda Piccoli Frasson, Camila Braz Menezes, Gustavo Krumel Goelzer, Simone Cristina Baggio Gnoatto, Solange Cristina Garcia, Tiana Tasca
Purinergic Signalling.2017; 13(4): 569. CrossRef
Trichomonas vaginalis α-Actinin 2 Modulates Host Immune Responses by Inducing Tolerogenic Dendritic Cells via IL-10 Production from Regulatory T Cells
Hye-Yeon Lee, Juri Kim, Jae-Sook Ryu, Soon-Jung Park
The Korean Journal of Parasitology.2017; 55(4): 375. CrossRef
SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete
Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton
Infection and Immunity.2017;[Epub] CrossRef
OsmC and incomplete glycine decarboxylase complex mediate reductive detoxification of peroxides in hydrogenosomes of Trichomonas vaginalis
Eva Nývltová, Tamara Smutná, Jan Tachezy, Ivan Hrdý
Molecular and Biochemical Parasitology.2016; 206(1-2): 29. CrossRef
Symbiotic Association with Mycoplasma hominis Can Influence Growth Rate, ATP Production, Cytolysis and Inflammatory Response of Trichomonas vaginalis
Valentina Margarita, Paola Rappelli, Daniele Dessì, Gianfranco Pintus, Robert P. Hirt, Pier L. Fiori
Frontiers in Microbiology.2016;[Epub] CrossRef
Trichomoniasis immunity and the involvement of the purinergic signaling
Camila Braz Menezes, Tiana Tasca
Biomedical Journal.2016; 39(4): 234. CrossRef
Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products
Min-Ji Song, Jong-Joo Lee, Young Hee Nam, Tae-Gyun Kim, Youn Wook Chung, Mikyoung Kim, Ye-Eun Choi, Myeong Heon Shin, Hyoung-Pyo Kim
BMB Reports.2015; 48(2): 103. CrossRef
Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with <i>Trichomonas vaginalis</i>
Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(4): 371. CrossRef
Analysis of the Oxidative Stress Status in Nonspecific Vaginitis and Its Role in Vaginal Epithelial Cells Apoptosis
Zhaojie Chen, Zhen Zhang, Haiyan Zhang, Beibei Xie
BioMed Research International.2015; 2015: 1. CrossRef
T. vaginalis Infection Is Associated with Increased IL-8 and TNFr1 Levels but with the Absence of CD38 and HLADR Activation in the Cervix of ESN
Olamide D. Jarrett, Kirsten E. Brady, Sharada P. Modur, Jill Plants, Alan L. Landay, Mahmood Ghassemi, Elizabeth T. Golub, Greg T. Spear, Richard M. Novak, Clive M. Gray
PLOS ONE.2015; 10(6): e0130146. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Immunity in urogenital protozoa
N. Malla, K. Goyal, R. S. Dhanda, M. Yadav
Parasite Immunology.2014; 36(9): 400. CrossRef
Differential Regulation of Proinflammatory Cytokine Expression by Mitogen-Activated Protein Kinases in Macrophages in Response to Intestinal Parasite Infection
Mei Xing Lim, Chin Wen Png, Crispina Yan Bing Tay, Joshua Ding Wei Teo, Huipeng Jiao, Norbert Lehming, Kevin Shyong Wei Tan, Yongliang Zhang, J. A. Appleton
Infection and Immunity.2014; 82(11): 4789. CrossRef
IL‐22 Levels are Associated with Trichomonas vaginalis Infection in the Lower Genital Tract
Hadijat Moradeke Makinde, Reza Zariffard, Paria Mirmonsef, Richard M Novak, Olamide Jarrett, Alan L Landay, Gregory T Spear
American Journal of Reproductive Immunology.2013; 70(1): 38. CrossRef
IL-10 release by bovine epithelial cells cultured with Trichomonas vaginalis and Tritrichomonas foetus
Ricardo Chaves Vilela, Marlene Benchimol
Memórias do Instituto Oswaldo Cruz.2013; 108(1): 110. CrossRef
Involvement of purinergic signaling on nitric oxide production by neutrophils stimulated with Trichomonas vaginalis
Amanda Piccoli Frasson, Geraldo Attilio De Carli, Carla Denise Bonan, Tiana Tasca
Purinergic Signalling.2012; 8(1): 1. CrossRef
Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products
Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin
Inflammation Research.2012; 61(2): 97. CrossRef
Involvement of mast cells in inflammation induced by Trichomonas vaginalis via crosstalk with vaginal epithelial cells
I. H. HAN, S. J. PARK, M. H. AHN, J. S. RYU
Parasite Immunology.2012; 34(1): 8. CrossRef
Leukotriene B4 receptor BLT-mediated phosphorylation of NF-κB and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products
Young Hee Nam, Deulle Min, Hyoung-Pyo Kim, Kyoung-Ju Song, Kyeong Ah Kim, Young Ah Lee, Seong Hoon Kim, Myeong Heon Shin
Microbes and Infection.2011; 13(14-15): 1211. CrossRef
NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced byTrichomonas vaginalis-Derived Secretory Products
Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2011; 49(3): 291. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef

11,804 View
118 Download
Crossref

Double-stranded RNA virus in Korean Isolate IH-2 of Trichomonas vaginalis: Jong Wook Kim, Pyung-Rim Chung, Myung-Ki Hwang, Eun Young Choi; Korean J Parasitol 2007;45(2):87-94.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.87

Abstract
PDF

In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.

Citations

Citations to this article as recorded by

ՏՐԻԽՈՄՈՆԱՍ ՎԱԳԻՆԱԼԻՍ ՎԻՐՈՒՍԻ (TVV) ԱԶԴԵՑՈՒԹՅՈՒՆԸ ԿԱՆԱՆՑ ՄԻԶԱՍԵՌԱԿԱՆ ՏՐԻԽՈՄՈՆԻԱԶԻ ՎՐԱ
G.K. Ghardyan
MEDICINE, SCIENCE AND EDUCATION.2024; (37): 70. CrossRef
Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae)
Alexandra Ibañez-Escribano, Maria Teresa Gomez-Muñoz, Marta Mateo, Cristina Fonseca-Berzal, Esperanza Gomez-Lucia, Raquel Garcia Perez, Jose M. Alunda, Javier Carrion
Veterinary Sciences.2024; 11(7): 321. CrossRef
Multiple Regulations of Parasitic Protozoan Viruses: A Double-Edged Sword for Protozoa
Zhiteng Zhao, Xin Li, Nan Zhang, Jianhua Li, Na Zhao, Mengyao Gao, Xichen Zhang, Xiaocen Wang, Panpan Zhao, Lu Li, Min Sun, Lili Cao, Pengtao Gong, Vinayaka R. Prasad
mBio.2023;[Epub] CrossRef
PROTOZOONLARIN VİRAL ENDOSİMBİYONTLARI
Ayşegül DAMLAPINAR, Kader YILDIZ
Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni.2023; 14(1): 25. CrossRef
Discovery of a Novel Species of Trichomonasvirus in the Human Parasite Trichomonas vaginalis Using Transcriptome Mining
Austin Manny, Carrie Hetzel, Arshan Mizani, Max Nibert
Viruses.2022; 14(3): 548. CrossRef
A systematic review and meta-analysis on the global status of Trichomonas vaginalis virus in Trichomonas vaginalis
Saeed Bahadory, Selva Aminizadeh, Ali Taghipour, Farah Bokharaei-Salim, Khadijeh Khanaliha, Mohammad Hossein Razizadeh, Alireza Soleimani, Leila beikzadeh, Alireza Khatami
Microbial Pathogenesis.2021; 158: 105058. CrossRef
Biosynthesis Products of Pseudomonas sp. PS-17 Strain Metabolites. 1. Obtaining and Thermal Characteristics
Ihor Semeniuk, Viktoria Kochubei, Volodymyr Skorokhoda, Tetyana Pokynbroda, Halyna Midyana, Elena Karpenko, Viktor Melnyk
Chemistry & Chemical Technology.2020; 14(1): 26. CrossRef
Trichomonas vaginalis virus: a review of the literature
KJ Graves, AP Ghosh, PJ Kissinger, CA Muzny
International Journal of STD & AIDS.2019; 30(5): 496. CrossRef
Novel Partitivirus Enhances Virulence of and Causes Aberrant Gene Expression in Talaromyces marneffei
Susanna K. P. Lau, George C. S. Lo, Franklin W. N. Chow, Rachel Y. Y. Fan, James J. Cai, Kwok-Yung Yuen, Patrick C. Y. Woo, John W. Taylor
mBio.2018;[Epub] CrossRef
Trichomonas vaginalis infection in symbiosis with Trichomonasvirus and Mycoplasma
Raina Fichorova, Jorge Fraga, Paola Rappelli, Pier Luigi Fiori
Research in Microbiology.2017; 168(9-10): 882. CrossRef
Double-stranded RNA viral infection of Trichomonas vaginalis (TVV1) in Iranian isolates
Khadijeh Khanaliha, Hossein Masoumi-Asl, Farah Bokharaei-Salim, Azardokht Tabatabaei, Mehri Naghdalipoor
Microbial Pathogenesis.2017; 109: 56. CrossRef
Molecular characterization of double-stranded RNA virus in Trichomonas vaginalis Egyptian isolates and its association with pathogenicity
Eman K. El-Gayar, Amira B. Mokhtar, Wael A. Hassan
Parasitology Research.2016; 115(10): 4027. CrossRef
High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas vaginalis clinical isolates in South Brazil
Débora da Luz Becker, Odelta dos Santos, Amanda Piccoli Frasson, Graziela de Vargas Rigo, Alexandre José Macedo, Tiana Tasca
Infection, Genetics and Evolution.2015; 34: 181. CrossRef
Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
Jorge Fraga, Lazara Rojas, Idalia Sariego, Ayme Fernández-Calienes
Infection, Genetics and Evolution.2012; 12(1): 113. CrossRef
Identification and characterization of a type III Trichomonas vaginalis virus in the protozoan pathogen Trichomonas vaginalis
Irina N. Bessarab, Rui Nakajima, Hsing-Wei Liu, Jung-Hsiang Tai
Archives of Virology.2011; 156(2): 285. CrossRef
Clinical Isolates of Trichomonas vaginalis Concurrently Infected by Strains of Up to Four Trichomonasvirus Species (Family Totiviridae)
Russell P. Goodman, Taylor S. Freret, Tomasz Kula, Alexander M. Geller, Megan W. T. Talkington, Vanessa Tang-Fernandez, Olimpia Suciu, Aleksander A. Demidenko, Said A. Ghabrial, David H. Beach, Bibhuti N. Singh, Raina N. Fichorova, Max L. Nibert
Journal of Virology.2011; 85(9): 4258. CrossRef

15,667 View
71 Download
Crossref

Brief Communication

Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions: Yong-Seok Kim, Hyun-Ouk Song, Ik-Hwa Choi, Soon-Jung Park, Jae-Sook Ryu; Korean J Parasitol 2006;44(4):373-378.
Published online December 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.4.373

Abstract
PDF

To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in iron-depleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC₆ also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.

Citations

Citations to this article as recorded by

Molecular Targets Implicated in the Antiparasitic and Anti-Inflammatory Activity of the Phytochemical Curcumin in Trichomoniasis
Natalia Mallo, Jesús Lamas, Rosa Ana Sueiro, José Manuel Leiro
Molecules.2020; 25(22): 5321. CrossRef
Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis
Hyun-Ouk Song
The Korean Journal of Parasitology.2016; 54(1): 71. CrossRef
Nitric oxide maintains cell survival of Trichomonas vaginalis upon iron depletion
Wei-Hung Cheng, Kuo-Yang Huang, Po-Jung Huang, Jo-Hsuan Hsu, Yi-Kai Fang, Cheng-Hsun Chiu, Petrus Tang
Parasites & Vectors.2015;[Epub] CrossRef
RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins
Elisa Figueroa-Angulo, Jaeson Calla-Choque, Maria Mancilla-Olea, Rossana Arroyo
Biomolecules.2015; 5(4): 3354. CrossRef
Optimal Reference Genes for Gene Expression Normalization in Trichomonas vaginalis
Odelta dos Santos, Graziela de Vargas Rigo, Amanda Piccoli Frasson, Alexandre José Macedo, Tiana Tasca, Robert W Dettman
PLOS ONE.2015; 10(9): e0138331. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Hydrogenosome Metabolism Is the Key Target for Antiparasitic Activity of Resveratrol against Trichomonas vaginalis
Natalia Mallo, Jesús Lamas, José M. Leiro
Antimicrobial Agents and Chemotherapy.2013; 57(6): 2476. CrossRef
Responsiveness of Trichomonas vaginalis to iron concentrations: Evidence for a post-transcriptional iron regulation by an IRE/IRP-like system
J.C. Torres-Romero, R. Arroyo
Infection, Genetics and Evolution.2009; 9(6): 1065. CrossRef
Proinflammatory Cytokine and Nitric Oxide Production by Human Macrophages Stimulated with Trichomonas vaginalis
Ik-Hwan Han, Sung Young Goo, Soon-Jung Park, Se-Jin Hwang, Yong-Seok Kim, Michael Sungwoo Yang, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2009; 47(3): 205. CrossRef
Trichomonas vaginalis: The adhesins AP51 and AP65 bind heme and hemoglobin
Shahed Ardalan, B. Craig Lee, Gary E. Garber
Experimental Parasitology.2009; 121(4): 300. CrossRef
Trichomonas vaginalis‐induced neutrophil apoptosis causes anti‐inflammatory cytokine production by human monocyte‐derived macrophages
M. H. AHN, H. O. SONG, J. S. RYU
Parasite Immunology.2008; 30(8): 410. CrossRef

9,269 View
113 Download
Crossref

Mini Review

Trichomonas vaginalis and trichomoniasis in the Republic of Korea: Jae-Sook Ryu, Duk-Young Min; Korean J Parasitol 2006;44(2):101-116.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.101

Abstract
PDF

Vaginal trichomoniasis, caused by Trichomonas vaginalis, is the most common sexually transmitted disease. More than 170 million people worldwide are annually infected by this protozoan. In the Republic of Korea, 10.4% of women complaining of vaginal symptoms and signs were found to be infected with T. vaginalis. However, despite its high prevalence, the pathogenesis of T. vaginalis infection has not been clearly characterized although neutrophil infiltration is considered to be primarily responsible for the cytologic changes associated with this infection. We hypothesized that trichomonads in the vagina sometime after an acute infection secrete proteins like excretory-secretory product that have a chemotactic effect on neutrophils, and that these neutrophils are further stimulated by T. vaginalis to produce chemokines like IL-8 and GRO-α, which further promote neutrophil recruitment and chemotaxis. Thus, neutrophil accumulation is believed to maintain or aggravate inflammation. However, enhanced neutrophil apoptosis induced by live T. vaginalis could contribute to resolution of inflammation. Macrophages may constitute an important component of host defense against T. vaginalis infection. For example, mouse macrophages alone and those activated by lymphokines or nitric oxide are known to be involved in the extracellular killing of T. vaginalis. In the host, T. vaginalis uses a capping phenomenon to cleave host immunoglobulins with proteinases and thus escape from host immune responses. Recently, we developed a highly sensitive and specific diagnostic polymerase chain reaction (PCR) technique using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650), and found that the method enables the detection of T. vaginalis at concentrations as low as 1 cell per PCR mixture.

Citations

Citations to this article as recorded by

Computational prediction of new therapeutic effects of probiotics
Sadegh Sulaimany, Kajal Farahmandi, Aso Mafakheri
Scientific Reports.2024;[Epub] CrossRef
Dynamin 2-mediated endocytosis of BLT1 is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
Young Ah Lee, Myeong Heon Shin
Parasites, Hosts and Diseases.2024; 62(3): 281. CrossRef
Endowed Polyphenols in Advanced Delivery Systems for Vaginal Infections
Eduardo Apolinário, Maria Leonor Castro, Manuela Pintado, João Paulo Ferreira, Sara Baptista-Silva, Sandra Borges
Applied Sciences.2024; 14(20): 9203. CrossRef
Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran
Maryam Alikhani, Mohammad Akhoundi, Denis Sereno, Jahangir Abdi, Razi Naserifar, Mohammad Reza Mahmoudi, Asad Mirzaei
Parasitology Research.2022; 121(6): 1631. CrossRef
Spatiotemporal Clusters and Trend of Trichomonas vaginalis Infection in Korea
Yeong Hoon Kim, Hye-Jin Ahn, Dongjae Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2022; 60(2): 97. CrossRef
Mast cell tryptase‐PAR2 pathway in proliferation of prostatic stromal cells reacted with Trichomonas vaginalis
Chang‐Suk Noh, Hyo‐Yeoung Chung, Ik‐Hwan Han, Jung‐Hyun Kim, Yu‐Mi Kim, Jae‐Sook Ryu
Parasite Immunology.2021;[Epub] CrossRef
Trichomoniasis in a tertiary hospital of Madrid, Spain (2013–2017): prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis
Celia Bolumburu, Vega Zamora, María Muñoz-Algarra, Francisca Portero-Azorín, José Antonio Escario, Alexandra Ibáñez-Escribano
Parasitology Research.2020; 119(6): 1915. CrossRef
Neutrophil interactions with the sexually transmitted parasite Trichomonas vaginalis: implications for immunity and pathogenesis
Suhani B. Bhakta, Jose A. Moran, Frances Mercer
Open Biology.2020;[Epub] CrossRef
Review of Successful Control of Parasitic Infections in Korea
Sung-Tae Hong, Tai-Soon Yong
Infection & Chemotherapy.2020; 52(3): 427. CrossRef
Viruses of protozoan parasites and viral therapy: Is the time now right?
Paul Barrow, Jean Claude Dujardin, Nicolas Fasel, Alex D. Greenwood, Klaus Osterrieder, George Lomonossoff, Pier Luigi Fiori, Robert Atterbury, Matteo Rossi, Marco Lalle
Virology Journal.2020;[Epub] CrossRef
Lactobacillus inersIs Associated with Vaginal Dysbiosis in Healthy Pregnant Women: A Preliminary Study
Nengneng Zheng, Renyong Guo, Yinyu Yao, Meiyuan Jin, Yiwen Cheng, Zongxin Ling
BioMed Research International.2019; 2019: 1. CrossRef
Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(1): 75. CrossRef
Epidemiology of Trichomoniasis in South Korea and Increasing Trend in Incidence, Health Insurance Review and Assessment 2009-2014
So-Young Joo, Youn-Kyoung Goo, Jae-Sook Ryu, Sang-Eun Lee, Won Kee Lee, Dong-Il Chung, Yeonchul Hong, Zhefeng Meng
PLOS ONE.2016; 11(12): e0167938. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Inhibitory effect of bee venom on the growth of Trichomonas vaginalis
Ji-Hae Kim, Jae-Sook Ryu, Mi-Young Lee
Toxicology and Environmental Health Sciences.2014; 6(1): 48. CrossRef
Evaluation of Seeplex® STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections
Seung-Ju Lee, Dong Sup Lee, Hyun-Sop Choe, Dong Choon Park, Yong-Hyun Cho
Journal of Infection and Chemotherapy.2012; 18(4): 494. CrossRef
Clinical Implications of Multiplex PCR Detection of Fastidious Microorganisms in Vaginitis Patients
Nae Yu, Mi-Kyung Lee
Korean Journal of Clinical Microbiology.2011; 14(1): 30. CrossRef
Lactobacillus acidophilusContributes to a Healthy Environment for Vaginal Epithelial Cells
Woojin Pi, Jae-Sook Ryu, Jaesook Roh
The Korean Journal of Parasitology.2011; 49(3): 295. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef
Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function
Bibhuti N. Singh, Gary R. Hayes, John J. Lucas, Ulf Sommer, Nelly Viseux, Ekaterina Mirgorodskaya, Radiana T. Trifonova, Rosaria Rita S. Sassi, Catherine E. Costello, Raina N. Fichorova
Glycoconjugate Journal.2009; 26(1): 3. CrossRef
A Case of Pulmonary Trichomoniasis in a Young Healthy Male
Sung Soo La, Jae Hwan Kong, Chang Seok Bang, Sung Hwhan Hahn, Na-Hye Myong, Doh Hyung Kim
Tuberculosis and Respiratory Diseases.2008; 64(5): 387. CrossRef

18,444 View
125 Download
Crossref

Original Articles

Degradations of human immunoglobulins and hemoglobin by a 60 kDa cysteine proteinase of Trichomonas vaginalis: Duk-Young Min, Keun-Hee Hyun, Jae-Sook Ryu, Myoung-Hee Ahn, Myung-Hwan Cho; Korean J Parasitol 1998;36(4):261-268.
Published online December 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.4.261

Abstract
PDF

The present study was undertaken to investigate the role of cysteine proteinase of Trichomonas vaginalis in escaping from host defense mechanism. A cysteine proteinase of T. vaginalis was purified by affinity chromatography and gel filtration. Optimum pH for the purified proteinase activity was 6.0. The proteinase was inhibited by cysteine and serine proteinase inhibitors such as E-64, NEM, IAA, leupeptin, TPCK and TLCK, and also by Hg²⁺, but not affected by serine-, metallo-, and aspartic proteinase inhibitors such as PMSF, EDTA and pepstatin A. However, it was activated by the cysteine proteinase activator, DTT. The molecular weight of a purified proteinase was 62 kDa on gel filtration and 60 kDa on SDS-PAGE. Interestingly, the purified proteinase was able to degrade serum IgA, secretory IgA, and serum IgG in time- and dose-dependent manners. In addition, the enzyme also degraded hemoglobin in a dose-dependent manner. These results suggest that the acidic cysteine proteinase of T. vaginalis may play a dual role for parasite survival in conferring escape from host humoral defense by degradation of immunoglobulins, and in supplying nutrients to parasites by degradation of hemoglobin.

Citations

Citations to this article as recorded by

Hemoglobin uptake and utilization by human protozoan parasites: a review
Magda Reyes-López, Beatriz Aguirre-Armenta, Carolina Piña-Vázquez, Mireya de la Garza, Jesús Serrano-Luna
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Microbiota in vaginal health and pathogenesis of recurrent vulvovaginal infections: a critical review
Namarta Kalia, Jatinder Singh, Manpreet Kaur
Annals of Clinical Microbiology and Antimicrobials.2020;[Epub] CrossRef
Immunopathology of Recurrent Vulvovaginal Infections: New Aspects and Research Directions
Namarta Kalia, Jatinder Singh, Manpreet Kaur
Frontiers in Immunology.2019;[Epub] CrossRef
Trichomonas vaginalis: pathogenicity and potential role in human reproductive failure
Ewelina Mielczarek, Joanna Blaszkowska
Infection.2016; 44(4): 447. CrossRef
Trichomoniasis immunity and the involvement of the purinergic signaling
Camila Braz Menezes, Tiana Tasca
Biomedical Journal.2016; 39(4): 234. CrossRef
Acanthamoeba castellanii Proteases are Capable of Degrading Iron‐Binding Proteins as a Possible Mechanism of Pathogenicity
Gerardo Ramírez‐Rico, Moisés Martínez‐Castillo, Mireya de la Garza, Mineko Shibayama, Jesús Serrano‐Luna
Journal of Eukaryotic Microbiology.2015; 62(5): 614. CrossRef
Biological roles of cysteine proteinases in the pathogenesis ofTrichomonas vaginalis
Hilda M. Hernández, Ricardo Marcet, Jorge Sarracent
Parasite.2014; 21: 54. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef
Identification and characterization of a surface-associated, subtilisin-like serine protease inTrichomonas vaginalis
PABLO HERNÁNDEZ-ROMANO, ROBERTO HERNÁNDEZ, ROSSANA ARROYO, JOHN F. ALDERETE, IMELDA LÓPEZ-VILLASEÑOR
Parasitology.2010; 137(11): 1621. CrossRef
Trichomonas vaginalis: The adhesins AP51 and AP65 bind heme and hemoglobin
Shahed Ardalan, B. Craig Lee, Gary E. Garber
Experimental Parasitology.2009; 121(4): 300. CrossRef
Induction of human host cell apoptosis by Trichomonas vaginalis cysteine proteases is modulated by parasite exposure to iron
Shelley Kummer, Gary R. Hayes, Robert O. Gilbert, David H. Beach, John J. Lucas, Bibhuti N. Singh
Microbial Pathogenesis.2008; 44(3): 197. CrossRef
Giardia duodenalis: protein substrates degradation by trophozoite proteases
Silvana Torossian Coradi, Semíramis Guimarães
Parasitology Research.2006; 99(2): 131. CrossRef
Trichomonas vaginalis and trichomoniasis in the Republic of Korea
Jae-Sook Ryu, Duk-Young Min
The Korean Journal of Parasitology.2006; 44(2): 101. CrossRef
Identification of Trichomonas vaginalis Cysteine Proteases That Induce Apoptosis in Human Vaginal Epithelial Cells
Ulf Sommer, Catherine E. Costello, Gary R. Hayes, David H. Beach, Robert O. Gilbert, John J. Lucas, Bibhuti N. Singh
Journal of Biological Chemistry.2005; 280(25): 23853. CrossRef
Trichomonas vaginalis: characterization of a 39-kDa cysteine proteinase found in patient vaginal secretions
Rodolfo Hernández-Gutiérrez, Leticia Avila-González, Jaime Ortega-López, Fernando Cruz-Talonia, Guillermo Gómez-Gutierrez, Rossana Arroyo
Experimental Parasitology.2004; 107(3-4): 125. CrossRef
Tritrichomonas foetus Induces Apoptotic Cell Death in Bovine Vaginal Epithelial Cells
B. N. Singh, J. J. Lucas, G. R. Hayes, Ish Kumar, D. H. Beach, Marcel Frajblat, R. O. Gilbert, U. Sommer, C. E. Costello
Infection and Immunity.2004; 72(7): 4151. CrossRef
Biology of trichomonosis
Michael W. Lehker, John F. Alderete
Current Opinion in Infectious Diseases.2000; 13(1): 37. CrossRef

9,191 View
60 Download
Crossref

Biological and biochemical modulation of Trichomonas vaginalis KT9 isolate after shifting of culture medium from TPS-1 into TYM: Jae-Sook Ryu, Ryung Choi, So-Young Park, Hyun Park, Duk-Young Min; Korean J Parasitol 1998;36(4):255-260.
Published online December 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.4.255

Abstract
PDF

To evaluate the biological and biochemical characteristics of Trichomonas vaginalis KT9 isolate, the growth and size of trichomonads, pathogenicity in mouse, protein profiles and proteinase activity were examined after shifting the medium from TPS-1 into TYM. Generation time of trichomonads in TYM medium was 4.5 hr in comparison to TPS-1 with 7.1 hr. Size of trichomonads cultured in TPS-1 medium (8.5 ± 0.9 × 6.0 ± 0.9 ?m) was significantly smaller than those in TYM medium (10.9 ± 1.4 × 8.2 ± 0.9 ?m). Trichomonads cultured in TYM medium produced subcutaneous abscess in 9 out of 10 mice, whereas those in TPS-1 medium produced abscesses in 2 out of 10 mice. In SDS-PAGE, trichomonad lysates from both media showed ten common bands. However, trichomonads in TYM medium showed additional bands of 136 kDa, 116 kDa and 40 kDa in comparison to those in TPS-1 with 100 kDa. By immunoblot with T. vaginalis-immunized rabbit sera, T. vaginalis cultivated in both TYM and TPS-1 media showed 5 common bands, and unique bands of 116 kDa, 105 kDa, and 86 kDa were observed in trichomonads in TYM while a 140 kDa band in those in TPS-1. In gelatin SDS-PAGE, trichomonads in TYM degraded gelatin stronger than those in TPS-1. Also protease activity of trichomonads in TYM was significantly higher than that of trichomonads in TPS-1 using Bz-Pro-Phe-Arg-Nan as a substrate. According to the results, it is assumed that the shift from TPS-1 into TYM medium for cultivation of T. vaginalis might modulate the biological and biochemical properties of T. vaginalis in vitro.

Citations

Citations to this article as recorded by

Inhibitory effect of bee venom on the growth of Trichomonas vaginalis
Ji-Hae Kim, Jae-Sook Ryu, Mi-Young Lee
Toxicology and Environmental Health Sciences.2014; 6(1): 48. CrossRef
The Dimension of Trichomonas vaginalis as Measured by Scanning Electron Microscopy
Sang-Hoon Cheon, Seung Ryong Kim, Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu
The Korean Journal of Parasitology.2013; 51(2): 243. CrossRef
Growth kinetics, antigen profiling, and proteinase activity of Egyptian Trichomonas tenax isolates derived from patients having oral infections
Mahmoud M. El Sibaei, Nashwa S. Abdel-Fattah, Sabah A. Ahmed, Hanan M. Abou-Seri
Experimental Parasitology.2012; 130(4): 416. CrossRef
Phenotypic ‘variant’ forms of Trichomonas vaginalis trophozoites from cervical neoplasia patients
Afzan M. Yusof, Suresh Kumar
Experimental Parasitology.2012; 131(3): 267. CrossRef

7,256 View
74 Download
Crossref

Brief Communication

Genetic variance of Trichomonas vaginalis isolates by Southern hybridization: Jae-Sook Ryu, Duk-Young Min, Myeong-Heon Shin, Youl-Hee Cho; Korean J Parasitol 1998;36(3):207-211.
Published online September 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.3.207

Abstract
PDF

In the present study, genomic DNAs were purified from Korean isolates (KT8, KT6, KT-Kim and KT-Lee) and foreign strains (CDC85, IR78 and NYH 286) of Trichomonas vaginalis, and hybridized with a probe based on the repetitive sequence cloned from T. vaginalis to observe the genetic differences. By Southern hybridization, all isolates of T. vaginalis except the NYH286 strain had 11 bands. Therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) KT8, KT6 and KT-Kim isolates had 11 identical bands such as 1 kb, 1.2 kb, 1.6 kb, 1.9 kb, 2.3 kb, 2.7 kb, 3.2 kb, 3.4 kb, 3.8 kb, 4.9 kb and 6.0 kb. ii) The metronidazole-resistant IR78 strain had the same bands as KT-Lee isolate at bands of 1 kb, 1.2 kb, 1.6 kb, 1.8 kb, 2.1 kb, 2.5 kb, 2.7 kb, 2.9 kb, 3.4 kb, 5.0 kb and 6.0 kb. Bands of CDC85, metronidazole-resistant strain, were similar to those of IR78 and KT-Lee, except that 3.2 kb replaced 2.9 kb. iii) NYH286 particularly had 12 bands and band patterns were similar to IR78 with a few exceptions as follows: i) 6.2 kb in place of 6.0 kb, ii) 2.0 kb and 2.2 kb instead of 2.1 kb. Through the results obtained, genetic variance of T. vaginalis isolates was demonstrated by Southern hybridization.

Citations

Citations to this article as recorded by

Population structure and genetic diversity of Trichomonas vaginalis clinical isolates in Australia and Ghana
Daniel S. Squire, Alan J. Lymbery, Jennifer Walters, Frances Brigg, Andrea Paparini, R.C. Andrew Thompson
Infection, Genetics and Evolution.2020; 82: 104318. CrossRef
Genotypic Variation in Trichomonas vaginalis Detected in South African Pregnant Women
Rennisha Chetty, Nonkululeko Mabaso, Nathlee Abbai
Infectious Diseases in Obstetrics and Gynecology.2020; 2020: 1. CrossRef
Genetic Diversity of Trichomonas Vaginalis Clinical Isolates According to Restriction Fragment Length Polymorphism Analysis of the 60-kDa Proteinase Gene
Hilda Hernández, Jorge Fraga, Ricardo Marcet, Annia Alba, Mabel Figueredo, Yenisey Alfonso, Lázara Rojas, Jorge Sarracent
Acta Parasitologica.2019; 64(2): 300. CrossRef
Genetic diversity inTrichomonas vaginalis
John C Meade, Jane M Carlton
Sexually Transmitted Infections.2013; 89(6): 444. CrossRef
Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing
Denise C. Cornelius, D. Ashley Robinson, Christina A. Muzny, Leandro A. Mena, David M. Aanensen, William B. Lushbaugh, John C. Meade
Journal of Clinical Microbiology.2012; 50(10): 3293. CrossRef
Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates
Jorge Fraga, Lazara Rojas, Idalia Sariego, Ayme Fernández-Calienes
Experimental Parasitology.2011; 127(2): 593. CrossRef
Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation
Lazara Rojas, Jorge Fraga, Idalia Sariego
Infection, Genetics and Evolution.2004; 4(1): 53. CrossRef

8,749 View
58 Download
Crossref

Original Article

PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea: Kim, Yun Ah , Kim, Hye Youn , Cho, Shin Hyeong , Cheun, Hyeong Il , Yu, Jae Ran , Lee, Sang Eun; Korean J Parasitol 2010;48(1):9-13.; DOI: https://doi.org/10.3347/kjp.2010.48.1.9

Abstract
PDF

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.

Citations

Citations to this article as recorded by

Development of a single-cell cloning technique for isolation of Pentatrichomonas hominis: a promising tool for diagnosing Trichomonas spp. infections in the pig breeding industry
Yibin Zhu, Haiming Cai, Shenquan Liao, Juan Li, Siyun Fang, Hanqin Shen, Dingai Wang, Zhuanqiang Yan, Minna Lv, Xuhui Lin, Junjing Hu, Yongle Song, Xiangjie Chen, Lijun Yin, Jianfei Zhang, Nanshan Qi, Mingfei Sun
Parasites & Vectors.2025;[Epub] CrossRef
Prevalence of Pentatrichomonas hominis infection in wild rodents
Yan Tang, Hai-Tao Wang, Jing-Hao Li, Qing-Yu Hou, Si-Yuan Qin, He Ma, Ya Qin, Quan Zhao, Hany M. Elsheikha, Shuo Liu
BMC Veterinary Research.2025;[Epub] CrossRef
Genetic diversity of Trichomonads from Milu deer (Elaphurus davidianus) in China
Yilei Zhang, Zhouchun Li, Xinglong Song, Guodong Xiao, Lingru He, Jiade Bai, Zhenyu Zhong, Lijie Tian, Yan Chang, Zhuang Li, Qingyun Guo, Congshan Yang, Qingxun Zhang
Parasite.2025; 32: 23. CrossRef
Epidemiology of Pentatrichomonas Hominis in Farmed Mink and Raccoon Dogs Across Five Provinces in China
Na Lu, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Xue-Min Li, Xing Yang, Jing Jiang, Shuo Liu
Foodborne Pathogens and Disease.2025;[Epub] CrossRef
Molecular Identification and Survey of Tetratrichomonas buttreyi and Pentatrichomonas hominis in Cattle in Shanxi Province, North China
Yu-Xuan Wang, Tao Jia, Zi-Rui Wang, Jin-Long Wang, Ze-Dong Zhang, Ze-Xuan Wu, Wen-Wei Gao, Xing-Quan Zhu, Qing Liu
Animals.2025; 15(13): 1899. CrossRef
Development of a novel one-tube LAMP-CRISPR/Cas12b technique for detecting Pentatrichomonas hominis
Qing-Xin Fan, Jin-Long Wang, Yao Liang, Yu-Xuan Wang, Han-Dan Xiao, Xing-Quan Zhu, Qing Liu
Microchemical Journal.2025; 215: 114488. CrossRef
Genetic diversity, prevalence and risk factors of trichomonads from captive non-human primates in China
Yilei Zhang, Yangfan Li, Jiawei Mi, Jiawen Zhang, Guodong Xiao, Siqi Xiong, Yew Hwong, Shibin Feng, Congshan Yang
Acta Tropica.2025; 270: 107816. CrossRef
Prevalence of Pentatrichomonas hominis in foxes and raccoon dogs and changes in the gut microbiota of infected female foxes in the Hebei and Henan Provinces in China
Pengtao Song, Yunan Guo, Shoujun Zuo, Liangliang Li, Fang Liu, Tao Zhang, Hongyu Dai, Haiju Dong
Parasitology Research.2024;[Epub] CrossRef
Fatal coinfection of blastocystosis and intestinal trichomoniasis in a rhesus macaque (Macaca mulatta)
Varun Kumar Sarkar, Ujjwal Kumar De, Pooja Solanki, Harshit Saxena, Shivansh Mehra, Devendra Prasad Pateer, Sudhir Kumar Prajapati
Journal of Parasitic Diseases.2024; 48(2): 400. CrossRef
Molecular Identification and Survey of Trichomonad Species in Pigs in Shanxi Province, North China
Zi-Rui Wang, Qing-Xin Fan, Jin-Long Wang, Shuo Zhang, Yu-Xuan Wang, Ze-Dong Zhang, Wen-Wei Gao, Xing-Quan Zhu, Qing Liu
Veterinary Sciences.2024; 11(5): 203. CrossRef
Morphological and molecular characterization of parabasilids isolated from ex situ nonhuman primates and their keepers at different institutions in Brazil
Lais Verdan Dib, Alynne da Silva Barbosa, Lais Lisboa Correa, Breno da Silva Torres, Alcides Pissinatti, Silvia Bahadian Moreira, Rodrigo Hidalgo Friciello Teixeira, André Luíz Mota da Costa, José Augusto Pereira Carneiro Muniz, Amauri Michel Junglos, Zel
International Journal for Parasitology: Parasites and Wildlife.2024; 24: 100946. CrossRef
Molecular-phylogenetic investigation of trichomonads in dogs and cats reveals a novel Tritrichomonas species
Barbara Tuska-Szalay, Julia Gilbert, Nóra Takács, Sándor A. Boldogh, József Fáy, Ágnes Sterczer, Roland Psáder, Jenő Kontschán, Ádám Izsó, Sándor Hornok
Parasites & Vectors.2024;[Epub] CrossRef
Prevalence of Intestinal Parasites in Dogs and Cats from the Kvarner Region in Croatia
Siniša Faraguna, Ivan Vlahek, Kristina Tea Miočić, Tibor Andreanszky, Marko Pećin
Acta Veterinaria.2023; 73(1): 41. CrossRef
Development of an LFD-RPA Assay for Rapid Detection of Pentatrichomonas hominis Infection in Dogs
Yao Rong, Xichen Zhang, Xuejiao Chen, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Taotao Yue, Hongbo Zhang, Xiaofei Zhou, Nan Zhang
Current Issues in Molecular Biology.2023; 45(11): 9252. CrossRef
Prevalence and molecular characterization of Pentatrichomonas hominis in Siberian tigers (Panthera tigris altaica) in northeast China
Hongbo ZHANG, Nan ZHANG, Pengtao GONG, Shuqin CHENG, Xiaocen WANG, Xin LI, Zhijun HOU, Chang LIU, Tianqi BI, Bobo WANG, Yidan CHENG, Jianhua LI, Xichen ZHANG
Integrative Zoology.2022; 17(4): 543. CrossRef
Identification of Pentatrichomonas hominis in preputial washes of bulls in Brazil
Otávia Reis e Silva, Laura Ribeiro, Vera Lucia Teixeira de Jesus, Douglas McIntosh, Lara Nogueira Silenciato, Joaquim Esquerdo Ferreira, Marco Roberto Bourg de Mello
Revista Brasileira de Parasitologia Veterinária.2022;[Epub] CrossRef
Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand
Aongart Mahittikorn, Ruenruetai Udonsom, Khuanchai Koompapong, Rachatawan Chiabchalard, Chantira Sutthikornchai, Preeyaporn Monatrakul Sreepian, Hirotake Mori, Supaluk Popruk
BMC Veterinary Research.2021;[Epub] CrossRef
Prevalence of Tetratrichomonas buttreyi and Pentatrichomonas hominis in yellow cattle, dairy cattle, and water buffalo in China
Wen-Chao Li, Jia-min Huang, Zhui Fang, Qi Ren, Li Tang, Zhen-zhen Kan, Xin-chao Liu, You-fang Gu
Parasitology Research.2020; 119(2): 637. CrossRef
Prevalence of Tritrichomonas foetus infection in cats in Bangkok metropolitan area and in vitro drug sensitivity testing
Alisara Leelanupat, Ketsarin Kamyingkird, Wissanuwat Chimnoi, Burin Nimsuphan
Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100440. CrossRef
A case of “misplaced” Tritrichomonas foetus infection in a dog in Northern Italy
Roberta Franchi, Walter Bertazzolo, Michele Marino, Barbara De Marco
Veterinary Parasitology: Regional Studies and Reports.2020; 22: 100451. CrossRef
Protozoan genital invasions caused by the representatives of trichomonas and giardia
Pavlo V. Fedorych, Gennadiy I. Mavrov, Tetiana V. Osinska, Yuliia V. Shcherbakova
Wiadomości Lekarskie.2020; 73(2): 380. CrossRef
Molecular prevalence of trichomonad species from pet shop puppies and kittens in Japan
Naoyuki Itoh, Yuko Iijima, Isao Ogura, Natsumi Yonekura, Satoshi Kameshima, Yuya Kimura
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
High prevalence of Pentatrichomonas hominis infection in gastrointestinal cancer patients
Nan Zhang, Hongbo Zhang, Yanhui Yu, Pengtao Gong, Jianhua Li, Ziyi Li, Ting Li, Zhanjie Cong, Chunying Tian, Xiaofeng Liu, Xiuyan Yu, Xichen Zhang
Parasites & Vectors.2019;[Epub] CrossRef
Pentatrichomonas hominis infection in two domestic cats with chronic diarrhea
Bethânia Ferreira Bastos, Beatriz Brener, Mariana Alves de Figueiredo, Daniela Leles, Flavya Mendes-de-Almeida
Journal of Feline Medicine and Surgery Open Reports.2018;[Epub] CrossRef
Prevalence of Pentatrichomonas hominis infections in six farmed wildlife species in Jilin, China
Xianhe Li, Jianhua Li, Xichen Zhang, Zhengtao Yang, Ju Yang, Pengtao Gong
Veterinary Parasitology.2017; 244: 160. CrossRef
Pentatrichomonas hominis: prevalence and molecular characterization in humans, dogs, and monkeys in Northern China
Wen-Chao Li, Meng Ying, Peng-Tao Gong, Jian-Hua Li, Ju Yang, He Li, Xi-Chen Zhang
Parasitology Research.2016; 115(2): 569. CrossRef
Prevalence and Molecular Characterization of Intestinal Trichomonads in Pet Dogs in East China
Wen-Chao Li, Kai Wang, Wei Zhang, Jingjing Wu, You-Fang Gu, Xi-Chen Zhang
The Korean Journal of Parasitology.2016; 54(6): 703. CrossRef
Detection of Pentatrichomonas hominis in dogs using real-time PCR
M. Michalczyk, R. Sokół, P. Socha
Polish Journal of Veterinary Sciences.2015; 18(4): 775. CrossRef
Molecular and morphologic identification of Pentatrichomonas hominis in swine
Weizhi Li, Wei Li, Pengtao Gong, Ying Meng, Wenchao Li, Chengcai Zhang, Shijie Li, Ju Yang, He Li, Xichen Zhang, Jianhua Li
Veterinary Parasitology.2014; 202(3-4): 241. CrossRef
Pentatrichomonas hominis: first isolation from the feces of a dog with diarrhea in China
Wen-Chao Li, Peng-Tao Gong, Meng Ying, Jian-Hua Li, Ju Yang, He Li, Zheng-Tao Yang, Guo-Cai Zhang, Xi-Chen Zhang
Parasitology Research.2014; 113(5): 1795. CrossRef
Molecular characterization of trichomonads isolated from animal hosts in the Philippines
Kris Genelyn B. Dimasuay, Windell L. Rivera
Veterinary Parasitology.2013; 196(3-4): 289. CrossRef
New Hosts ofSimplicimonas similisandTrichomitus batrachorumIdentified by 18S Ribosomal RNA Gene Sequences
Kris Genelyn B. Dimasuay, Orlie John Y. Lavilla, Windell L. Rivera
Journal of Parasitology Research.2013; 2013: 1. CrossRef
PCR amplification and DNA sequence analysis of parasitic intestinal protozoa in specimens stained with Chlorazol Black E
Norihito Morimoto, Masataka Korenaga, Yoshie Nishida, Hiroaki Takeuchi, Yoshitaka Kumon, Tetsuro Sugiura
Acta Parasitologica.2013;[Epub] CrossRef
Prevalence, risk factors of infection and molecular characterization of trichomonads in puppies from French breeding kennels
Aurélien Grellet, BrunoPolack, Alexandre Feugier, Corine Boucraut-Baralon, Dominique Grandjean, Laurine Vandewynckel, Amandine Cian, Dionigia Meloni, Eric Viscogliosi
Veterinary Parasitology.2013; 197(3-4): 418. CrossRef
Species identification of trichomonads and associated coinfections in dogs with diarrhea and suspected trichomonosis
M.K. Tolbert, C.M. Leutenegger, R. Lobetti, J. Birrell, J.L. Gookin
Veterinary Parasitology.2012; 187(1-2): 319. CrossRef
Identification of putative miRNAs from the deep-branching unicellular flagellates
Po-Jung Huang, Wei-Chen Lin, Shih-Chieh Chen, Yong-Han Lin, Chin-Hung Sun, Ping-Chiang Lyu, Petrus Tang
Genomics.2012; 99(2): 101. CrossRef
Molecular identification of Pentatrichomonas hominis in two patients with gastrointestinal symptoms
Dionigia Meloni, Cléa Mantini, Julien Goustille, Guillaume Desoubeaux, Zoha Maakaroun-Vermesse, Jacques Chandenier, Nausicaa Gantois, Christophe Duboucher, Pier Luigi Fiori, Eduardo Dei-Cas, Thanh Hai Duong, Eric Viscogliosi
Journal of Clinical Pathology.2011; 64(10): 933. CrossRef
Update on the Diagnosis and Management of Tritrichomonas foetus Infections in Cats
Kristin Manning
Topics in Companion Animal Medicine.2010; 25(3): 145. CrossRef

12,109 View
116 Download
Crossref

Brief Communication

Prevalence and Comparison of Diagnostic Methods for Trichomonas vaginalis Infection in Pregnant Women in Argentina: Perazzi, Beatriz E , Menghi, Claudia I , Coppolillo, Enrique F , Gatta, Claudia , Eliseth, Martha Cora , de Torres, Ramon A , Vay, Carlos A , Famiglietti, Angela M; Korean J Parasitol 2010;48(1):61-65.; DOI: https://doi.org/10.3347/kjp.2010.48.1.61

Abstract
PDF

The
objective
s of this study were to conduct a prevalence survey of trichomoniasis in pregnant women and to evaluate the utility of different methods for its diagnosis. A total of 597 vaginal exudates from pregnant women who were examined at the Hospital de Clinicas in Buenos Aires, Argentina from 1 August 2005 to 31 January 2007, were prospectively and consecutively evaluated. The investigation of Trichomonas vaginalis was made by different microscopic examinations, and culture on liquid medium. The sensitivity and specificity of the microscopic examinations were assessed considering culture on liquid medium as the "gold standard". The prevalence of T. vaginalis obtained by culture on liquid medium was 4.0% (24/597). The prevalence of T. vaginalis obtained by direct wet smear, prolonged May-Grunwald Giemsa staining, and sodium acetate-formalin (SAF)/methylene blue staining-fixing technique was 1.8%, 2.3% and 2.5%, respectively. The sensitivity of the direct wet smear was 45.8%, that of the prolonged May-Grunwald Giemsa staining was 58.3%, and that of the SAF/methylene blue method was 62.5%. Considering the 3 microscopic examinations altogether, the sensitivity rose to 66.7% and the specificity was 100% for all of them. This is the first time that the prevalence data of T. vaginalis by culture in pregnant women are published in Argentina. Due to the low sensitivity obtained by microscopy in asymptomatic pregnant women, the use of the liquid medium is recommended during pregnancy, in order to provide an early diagnosis and treatment.

Citations

Citations to this article as recorded by

Diagnostic utility of vaginal cytology compared to wet smear cytology for detection of Trichomonas vaginalis infection among adult women at a tertiary care center: A diagnostic evaluation study
Kamlesh Yadava, Pankaj Kumar Chaudhary, Amit Kumar, Mohd Arshad
MRIMS Journal of Health Sciences.2025; 13(2): 62. CrossRef
Microbiota unbalance in relation to high-risk human papillomavirus cervical infection
Juan Facundo Gomez Cherey, Sandra Noemi Payalef, Laura Fleider, Ana Paula Reyes, Verónica Andrea Maldonado, Mirta Olga Losada, Xin Chen, Lucia Helena Cardinal, Youxiang Wang, Silvio Alejandro Tatti, Beatriz Elizabeth Perazzi
International Journal of Gynecological Cancer.2023; 33(4): 482. CrossRef
Vaginal States: Detection of Conversion Processes in Women Using Contraception and Characterization of Vaginal Lactobacillus Species
Sonia Elena María Fosch, Marta Lucia Trossero, Omar Agustin Grosso, Ana Paula Reyes, Silvina Ema Cocucci, Sandra Noemí Payalef, Beatriz Elizabeth Perazzi
Infectious Disorders - Drug Targets.2022;[Epub] CrossRef
Comparison of diagnostic methods and analysis of socio-demographic factors associated with Trichomonas vaginalis infection in Sri Lanka
Sayuri Herath, Thivya Balendran, Akila Herath, Devika Iddawela, Susiji Wickramasinghe, Ali Rostami
PLOS ONE.2021; 16(10): e0258556. CrossRef
Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran
Hajar Ziaei Hezarjaribi, Mahbobeh Taghavi, Karan Hasanjani Saravi, Roghiyeh Faridnia, Hamed Kalani, Ahmad Mardani, Ogholniaz Jorjani, Zahra Hosseinikhah, Bahman Rahimi Esboei, Mehran Gholami, Mahdi Fakhar
Acta Parasitologica.2020; 65(4): 859. CrossRef
Bacterial vaginosis, vulvovaginal candidiasis and trichomonal vaginitis among reproductive-aged women seeking primary healthcare in Sana’a city, Yemen
Maha Abdul-Aziz, Mohammed A. K. Mahdy, Rashad Abdul-Ghani, Nuha A. Alhilali, Leena K. A. Al-Mujahed, Salma A. Alabsi, Fatima A. M. Al-Shawish, Noura J. M. Alsarari, Wala Bamashmos, Shahad J. H. Abdulwali, Mahdi Al Karawani, Abdullah A. Almikhlafy
BMC Infectious Diseases.2019;[Epub] CrossRef
Contraception: Influence on Vaginal Microbiota and Identification of Vaginal Lactobacilli Using MALDI-TOF MS and 16S rDNA Sequencing
Sonia E. Fosch, Cecilia A. Ficoseco, Antonella Marchesi, Silvina Cocucci, Maria E. F. Nader-Macias, Beatriz E. Perazzi
The Open Microbiology Journal.2018; 12(1): 218. CrossRef
Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina
Pamela Testardini, María Lucía Gallo Vaulet, Andrea Carolina Entrocassi, Claudia Menghi, Martha Cora Eliseht, Claudia Gatta, Mirta Losada, María Sol Touzón, Ana Corominas, Carlos Vay, Silvio Tatti, Angela Famiglietti, Marcelo Rodriguez Fermepin, Beatriz P
The Korean Journal of Parasitology.2016; 54(2): 191. CrossRef
Trichomoniasis in Pregnant Sudanese Pregnant Women at Tertiary Obstetric Facility, Khartoum, Sudan
Hanan A. O. Abd Allha, A. Fazari, Khalid Y. M. Ahmed
Open Journal of Obstetrics and Gynecology.2016; 06(09): 539. CrossRef
Prevalence of Trichomoniasis Among Women in Kermanshah, Iran
Naser Nazari, Maryam Zangeneh, Fatemeh Moradi, Arezoo Bozorgomid
Iranian Red Crescent Medical Journal.2015;[Epub] CrossRef
Evaluación de la disfunción vaginal en mujeres embarazadas sintomáticas y asintomáticas mediante la utilización de los estados vaginales básicos (EVB) y su comparación con el estudio microbiológico convencional
María S. Touzon, Mirta Losada, Martha Cora Eliseht, Claudia Menghi, Claudia Gatta, Gabriela Santa Cruz, Hilda Malamud de Ruda Vega, Carlos Vay, Silvio Tatti, Ángela Famiglietti, Beatriz Perazzi
Revista Argentina de Microbiología.2014; 46(3): 182. CrossRef
Prevalence of Trichomonas vaginalis in Pregnant Women in Zanjan, Northwest of Iran
Abbasali Nourian, Nushin Shabani, Asghar Fazaeli, Seyed Noraddin Mousavinasab
Jundishapur Journal of Microbiology.2013;[Epub] CrossRef
Codetection ofTrichomonas vaginalisandCandida albicansby PCR in Urine Samples in a Low-Risk Population Attended in a Clinic First Level in Central Veracruz, Mexico
A. López-Monteon, F. S. Gómez-Figueroa, G. Ramos-Poceros, D. Guzmán-Gómez, A. Ramos-Ligonio
BioMed Research International.2013; 2013: 1. CrossRef
Trichomonas vaginalis Infection among Pregnant Women in Jimma University Specialized Hospital, Southwest Ethiopia
Abdurehman Eshete, Zeleke Mekonnen, Ahmed Zeynudin
ISRN Infectious Diseases.2013; 2013: 1. CrossRef