Parasites, Hosts and Diseases

"PCR"

Brief Communication

Detection of trichomonads in induced sputum from asthma patients in Korea: Myung-hee Yi, Myungjun Kim, Jun Ho Choi, Yoon Hee Cho, Hyun Kyung Oh, Jung-Won Park, Ju Yeong Kim; Parasites Hosts Dis 2025;63(3):254-257.
Published online August 20, 2025; DOI: https://doi.org/10.3347/PHD.25028

Abstract
PDF

Trichomonads are flagellated protozoa that have occasionally been detected in the human respiratory tract, although detection rates have often been underestimated. We applied a nested PCR assay targeting the 18S rRNA gene of trichomonads to induced sputum from asthma patients to determine the prevalence of Trichomonas. Induced sputum was collected from 41 adults with asthma and analyzed through nested PCR using broad-range trichomonad primers and DNA sequencing for species identification. Nested PCR detected trichomonad DNA in 10 of the 41 (24.4%) samples. Sequencing and phylogenetic analysis revealed Trichomonas tenax in 8 cases and Tetratrichomonas sp. in 2 cases. These findings indicate that trichomonads can be present in the lower airways of patients with asthma, warranting further investigation into their clinical relevance.

1,441 View
43 Download

Original Article

Molecular cloning, identification, transcriptional analysis, and silencing of enolase on the life cycle of Haemaphysalis longicornis (Acari, Ixodidae) tick: Md. Samiul Haque, Md. Khalesur Rahman, Mohammad Saiful Islam, Myung-Jo You; Parasites Hosts Dis 2024;62(2):226-237.
Published online May 27, 2024; DOI: https://doi.org/10.3347/PHD.24015

Ticks, blood-sucking ectoparasites, spread diseases to humans and animals. Haemaphysalis longicornis is a significant vector for tick-borne diseases in medical and veterinary contexts. Identifying protective antigens in H. longicornis for an anti-tick vaccine is a key tick control strategy. Enolase, a multifunctional protein, significantly converts D-2-phosphoglycerate and phosphoenolpyruvate in glycolysis and gluconeogenesis in cell cytoplasm. This study cloned a complete open reading frame (ORF) of enolase from the H. longicornis tick and characterized its transcriptional and silencing effect. We amplified the full-length cDNA of the enolase gene using rapid amplification of cDNA ends. The complete cDNA, with an ORF of 1,297 nucleotides, encoded a 432-amino acid polypeptide. Enolase of the Jeju strain H. longicornis exhibited the highest sequence similarity with H. flava (98%), followed by Dermacentor silvarum (82%). The enolase motifs identified included N-terminal and C-terminal regions, magnesium binding sites, and several phosphorylation sites. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that enolase mRNA transcripts were expressed across all developmental stages of ticks and organs such as salivary gland and midgut. RT-PCR showed higher transcript levels in syn-ganglia, suggesting that synganglion nerves influence enolase,s role in tick salivary glands. We injected enolase double-stranded RNA into adult unfed female ticks, after which they were subsequently fed with normal unfed males until they spontaneously dropped off. RNA interference significantly (P<0.05) reduced feeding and reproduction, along with abnormalities in eggs (no embryos) and hatching. These findings suggest enolase is a promising target for future tick control strategies.

Citations

Citations to this article as recorded by

Comprehensive antigen identification and comparative analysis: significant approaches for controlling Haemaphysalis longicornis ticks
Md. Samiul Haque, Bumseok Kim, Myung-Jo You
Journal of Veterinary Science.2025;[Epub] CrossRef
Microbiome Composition of Haemaphysalis flava in Korea and Diversity Analysis Based on Region, Developmental Stage, and Sex
Min Kyu Sang, Jie eun Park, Dae Kwon Song, Jun Yang Jeong, Chan‐Eui Hong, Hyeonjun Shin, Hyeok Lee, Kyoung Won Lee, Hee Ju Hwang, Hyun woo Kim, Seong Yoon Kim, Wook‐Gyo Lee, So Young Park, Se Won Kang, Jung Han Park, Bharat Bhusan Patnaik, Sung‐Jae Cha, S
Entomological Research.2025;[Epub] CrossRef
Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae)
Md Samiul Haque, Bumseok Kim, Myung-Jo You
Revista Brasileira de Parasitologia Veterinária.2025;[Epub] CrossRef

4,074 View
50 Download
Crossref

Brief Communication

Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR: Preeti Kumari, Swati Sinha, Renuka Gahtori, Afshana Quadiri, Paras Mahale, Deepali Savargaonkar, Veena Pande, Bina Srivastava, Himmat Singh, Anupkumar R Anvikar; Korean J Parasitol 2022;60(4):295-299.
Published online August 24, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.4.295

Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic tools are required for accurate treatment. Molecular diagnosis of malaria is commonly carried out by nested polymerase chain reaction (PCR) targeting 18S rRNA gene, while this technique involves long turnaround time and multiple steps leading to false positive results. To overcome these drawbacks, we compared highly sensitive cytochrome oxidase gene-based single-step multiplex reaction with 18S rRNA nested PCR. Cytochrome oxidase (cox) genes of P. falciparum (cox-III) and P. vivax (cox-I) were compared with 18S rRNA gene nested PCR and microscopy. Cox gene multiplex PCR was found to be highly specific and sensitive, enhancing the detection limit of mixed infections. Cox gene multiplex PCR showed a sensitivity of 100% and a specificity of 97%. This approach can be used as an alternative diagnostic method as it offers higher diagnostic performance and is amenable to high throughput scaling up for a larger sample size at low cost.

Citations

Citations to this article as recorded by

Therapeutic mucosal vaccination of herpes simplex virus type 2 infected guinea pigs with an adenovirus-based vaccine expressing the ribonucleotide reductase 2 and glycoprotein D induces local tissue-resident CD4+ and CD8+ TRM cells associated with protect
Afshana Quadiri, Swayam Prakash, Hawa Vahed, Jimmy Medhat Tadros, Miyo Sun, Kathy K. Hormi-Carver, Swena Jignesh Patel, Lbachir BenMohamed
Frontiers in Immunology.2025;[Epub] CrossRef
Validation of real-time PCR assays for detecting Plasmodium and Babesia DNA species in blood samples
Luz Helena Patiño, Sergio Castañeda, Milena Camargo, Li Yong Cao, Bernadette Liggayu, Alberto Paniz‐Mondolfi, Juan David Ramírez
Acta Tropica.2024; 258: 107350. CrossRef
Comparative Analysis of Multiplex/Semi-nested PCR and Microscopy for Accurate Human Malaria Species Diagnosis
Aram Khezri, Mehdi Nateghpour, Haleh Hanifian, Leila Farivar, Afsaneh Motevalli Haghi
Jundishapur Journal of Microbiology.2024;[Epub] CrossRef

4,060 View
152 Download
3 Web of Science
Crossref

Case Reports

Two Clinical Cases of Feline Hemoplasmosis in Korea: Young Ju Kim, Hyeona Bae, Sun Woo Shin, ARom Cho, Yeseul Jeon, Tae-Sung Hwang, Dong-In Jung, Dae Young Kim, Jun-Gu Kang, DoHyeon Yu; Korean J Parasitol 2022;60(2):127-131.
Published online April 20, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.2.127

Feline hemotropic mycoplasmosis (hemoplasmosis) is an infection of the red blood cells caused by the Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt). The existence of Mhf, CMhm, and CMt has been demonstrated in feral cats in Korea using molecular methods, but no clinical cases have yet been reported. This study reports 2 clinical cases of hemotropic mycoplasmosis caused by CMhm and CMt in 2 anemic cats. The first case was a client-owned intact female domestic shorthair cat that presented with fever, pale mucous membranes, and normocytic normochromic non-regenerative anemia. Prior to referral, an immunosuppressive prednisolone dose was administered at the local veterinary clinic for 1 month. The cat was diagnosed with high-grade alimentary lymphoma. Organisms were found on the surface of the red blood cells on blood smear examination. The second case was of a rescued cat that presented with dehydration and fever. The cat had normocytic normochromic non-regenerative anemia. Necropsy revealed concurrent feline infectious peritonitis. Polymerase chain reaction assay targeting 16S rRNA revealed CMhm infection in case 1 and dual infection of CMhm and CMt in case 2. Normocytic normochromic non-regenerative anemia was observed in both cats before and during the management of the systemic inflammation. This is the first clinical case report in Korea to demonstrate CMhm and CMt infections in symptomatic cats.

Citations

Citations to this article as recorded by

Molecular Investigation and Characterization of Mycoplasma spp. infections in Naturally Infected Cats in Thailand
Kiattisak Pimpjong, Kanokwan Ratyotha, Thanvarat Rungruang, Napaporn Thongpriam, Kotchaphon Vaisusuk, Supawadee Piratae
Acta Parasitologica.2025;[Epub] CrossRef

5,957 View
199 Download
1 Web of Science
Crossref

Delayed Cerebral Toxoplasmosis in a Kidney Transplant Patient: a Case Report: Hosung Myeong, Moowan Park, Ji Eun Kim, Sun Won Park, Sang Hyung Lee; Korean J Parasitol 2022;60(1):35-38.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.35

Abstract
PDF

Cerebral toxoplasmosis is often life-threatening in an immunocompromised patient due to delayed diagnosis and treatment. Several differential diagnoses could be possible only with preoperative brain images of cerebral toxoplasmosis which show multiple rim-enhancing lesions. Due to the rarity of cerebral toxoplasmosis cases in Korea, the diagnosis and treatment are often delayed. This paper concerns a male patient whose cerebral toxoplasmosis was activated 21 years post kidney transplantation. Brain open biopsy was decided to make an exact diagnosis. Cerebral toxoplasmosis was confirmed by immunohistochemistry and PCR analyses of the tissue samples. Although cerebral toxoplasmosis was under control with medication, the patient did not recover clinically and died due to sepsis and recurrent gastrointestinal bleeding.

Citations

Citations to this article as recorded by

Disseminated toxoplasmosis infection 20 years post kidney transplant
Shaun Chandler, Lana Sundac, Carmel Hawley
BMJ Case Reports.2024; 17(9): e260412. CrossRef
Ciclosporin/mycophenolate mofetil

Reactions Weekly.2022; 1916(1): 146. CrossRef

3,851 View
202 Download
1 Web of Science
Crossref

Brief Communications

Clinical Usefulness of LabChip Real-time PCR using Lab-On-a-Chip Technology for Diagnosing Malaria: Jeeyong Kim, Da Hye Lim, Do-CiC Mihn, Jeonghun Nam, Woong Sik Jang, Chae Seung Lim; Korean J Parasitol 2021;59(1):77-82.
Published online February 19, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.1.77

Abstract
PDF

As malaria remains a major health problem worldwide, various diagnostic tests have been developed, including microscopy-based and rapid diagnostic tests. LabChip real-time PCR (LRP) is a small and portable device used to diagnose malaria using lab-on-a-chip technology. This study aimed to evaluate the diagnostic performance of LRP for detecting malaria parasites. Two hundred thirteen patients and 150 healthy individuals were enrolled from May 2009 to October 2015. A diagnostic detectability of LRP for malaria parasites was compared to that of conventional RT-PCR. Sensitivity of LRP for Plasmodium vivax, P. falciparum, P. malariae, and P. ovale was 95.5%, 96.0%, 100%, and 100%, respectively. Specificity of LRP for P. vivax, P. falciparum, P. malariae, and P. ovale was 100%, 99.3%, 100%, and 100%, respectively. Cohen’s Kappa coefficients between LRP and CFX96 for detecting P. vivax, P. falciparum, P. malariae, and P. ovale were 0.96, 0.98, 1.00, and 1.00, respectively. Significant difference was not observed between the results of LRP and conventional RT-PCR and microscopic examination. A time required to amplify DNAs using LRP and conventional RT-PCR was 27 min and 86 min, respectively. LRP amplified DNAs 2 times more fast than conventional RT-PCR due to the faster heat transfer. Therefore, LRP could be employed as a useful tool for detecting malaria parasites in clinical laboratories.

Citations

Citations to this article as recorded by

Current Trends in Nanotechnology-Based Drug Delivery Systems for the Diagnosis and Treatment of Malaria: A Review
Rohitas Deshmukh, Bhuvaneshwari Dewangan, Ranjit K. Harwansh, Rutvi Agrawal, Akash Garg, Himansu Chopra
Current Drug Delivery.2025; 22(3): 310. CrossRef
Parasitic protozoa: Biosystematics, identification, pathogenicity, causes and remedial measures
Gourav Mishra, Razia Kausar, Tushar Yadav
Microbial Pathogenesis.2025; 207: 107867. CrossRef
Internet-enabled lab-on-a-chip technology for education
Tyler Sano, Mohammad Julker Neyen Sampad, Jesus Gonzalez-Ferrer, Sebastian Hernandez, Samira Vera-Choqqueccota, Paola A. Vargas, Roberto Urcuyo, Natalia Montellano Duran, Mircea Teodorescu, David Haussler, Holger Schmidt, Mohammed A. Mostajo-Radji
Scientific Reports.2024;[Epub] CrossRef
Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review
Akua K. Yalley, Joyous Ocran, Jacob E. Cobbinah, Evangeline Obodai, Isaac K. Yankson, Anna A. Kafintu-Kwashie, Gloria Amegatcher, Isaac Anim-Baidoo, Nicholas I. Nii-Trebi, Diana A. Prah
Tropical Medicine and Infectious Disease.2024; 9(9): 190. CrossRef
Review of the Current Landscape of the Potential of Nanotechnology for Future Malaria Diagnosis, Treatment, and Vaccination Strategies
Arnau Guasch-Girbau, Xavier Fernàndez-Busquets
Pharmaceutics.2021; 13(12): 2189. CrossRef

5,127 View
132 Download
5 Web of Science
Crossref

Outbreak of Cyclosporiasis in Korean Travelers Returning from Nepal: Da-Won Ma, Myoung-Ro Lee, Bora Ku, Shin-Hyeong Cho, Sang-Eun Lee; Korean J Parasitol 2020;58(5):589-592.
Published online October 22, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.5.589

Abstract
PDF

Cyclospora cayetanensis is an apicomplexan protozoan and is one of the most common pathogens causing chronic diarrhea worldwide. Eight stool samples with diarrheal symptom out of 18 Korean residents who traveled to Nepal were obtained, and examined for 25 enteropathogens including 16 bacterial species, 5 viral species, and 4 protozoans in stool samples as causative agents of water-borne and food-borne disease. Only C. cayetanensis was detected by nested PCR, and 3 PCR-positive samples were sequenced to confirm species identification. However, the oocysts of C. cayetanensis in fecal samples could not be detected by direct microscopy of the stained sample. As far as we know, this is the first report of a group infection with C. cayetanensis from a traveler visiting Nepal, and the second report of a traveler’s diarrhea by C. cayetanensis imported in Korea.

Citations

Citations to this article as recorded by

Food Safety in Developing Countries: Common Foodborne and Waterborne Illnesses, Regulations, Organizational Structure, and Challenges of Food Safety in the Context of Nepal
Deepak Subedi, Madhav Paudel, Sandesh Poudel, Niranjan Koirala
Food Frontiers.2025; 6(1): 86. CrossRef
Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Cyclospora and Cyclosporiasis: The Nepalese Perspective
Pitambar Dhakal, Junqiang Li, Bishnu Maya K.C., Yin Fu, Yuancai Chen, Longxian Zhang
Zoonoses.2024;[Epub] CrossRef
Cyclospora cayetanensis: A Perspective (2020–2023) with Emphasis on Epidemiology and Detection Methods
Sonia Almeria, Leonor Chacin-Bonilla, Jenny G. Maloney, Monica Santin
Microorganisms.2023; 11(9): 2171. CrossRef
Waterborne transmission of protozoan parasites: a review of worldwide outbreaks – an update 2017–2022
Pavlina Bourli, Aida Vafae Eslahi, Ourania Tzoraki, Panagiotis Karanis
Journal of Water and Health.2023; 21(10): 1421. CrossRef
Waterborne protozoan outbreaks: An update on the global, regional, and national prevalence from 2017 to 2020 and sources of contamination
Jun-Yang Ma, Man-Yao Li, Ze-Zheng Qi, Ming Fu, Tian-Feng Sun, Hany M. Elsheikha, Wei Cong
Science of The Total Environment.2022; 806: 150562. CrossRef
Review on Cyclosporiasis Outbreaks and Potential Molecular Markers for Tracing Back Investigations
Junqiang Li, Feifei Xu, Md Robiul Karim, Longxian Zhang
Foodborne Pathogens and Disease.2022; 19(12): 796. CrossRef

4,738 View
99 Download
7 Web of Science
Crossref

Original Articles

Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea: Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho; Korean J Parasitol 2020;58(3):257-265.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.257

Abstract
PDF

The outbreak of human toxoplasmosis can be attributed to ingestion of food contaminated with Toxoplasma gondii. Toxoplasmosis recently increased in domestic and stray dogs and cats. It prompted studies on the zoonotic infectious diseases transmitted via these animals. Sero- and antigen prevalences of T. gondii in dogs and cats were surveyed using ELISA and PCR, and B1 gene phylogeny was analyzed in this study. Toxoplasmosis antibodies were measured on sera of 403 stray cats, 947 stray dogs, 909 domestic cats, and 2,412 domestic dogs collected at nationwide regions, Korea from 2017 to 2019. In addition, whole blood, feces, and tissue samples were also collected from stray cats (1,392), stray dogs (686), domestic cats (3,040), and domestic dogs (1,974), and T. gondii-specific B1 gene PCR was performed. Antibody prevalence of stray cats, stray dogs, domestic cats, and domestic dogs were 14.1%, 5.6%, 2.3%, and 0.04%, respectively. Antigen prevalence of these animals was 0.5%, 0.2%, 0.1%, and 0.4%, respectively. Stray cats revealed the highest infection rate of toxoplasmosis, followed by stray dogs, domestic cats, and domestic dogs. B1 gene positives were 5 of stray cats, and identified to high/moderate pathogenic Type I/III group. These findings enforce that preventive hygienic measure should be strengthened at One Health level in dogs and cats, domestic and stray, to minimize human toxoplasmosis infections.

Citations

Citations to this article as recorded by

Molecular detection of Toxoplasma gondii in ticks and their respective host dogs
Min-Goo Seo, Dongmi Kwak
Parasites, Hosts and Diseases.2025; 63(1): 66. CrossRef
Prevalence of parasitic infections in stray cats from Gimpo-si, Gyeonggi-do, Korea
Sooji Hong, Hyejoo Shin, Seungwan Ryoo, Chung-Won Lee, Jae-Young Park, Jong-Yil Chai, Bong-Kwang Jung
Parasites, Hosts and Diseases.2025; 63(2): 182. CrossRef
Toxoplasma gondii in owned and stray dogs from a Northwestern region of São Paulo State, Brazil: Seroprevalence and geospatial distribution from a One Health perspective
Fernando Henrique Antunes Murata, Jessica Priscilla Barboza, Fernanda Follis Tasso, Tainara Souza Pinho, Tiago Henrique, Janine Fusco Alves, Carlos Alexandre Guimarães de Souza, Daniel Abrahão, Ubirajara Leoncy de Lavor, Luiz Carlos de Mattos, Chunlei Su,
One Health.2025; 21: 101222. CrossRef
A 20-year serological survey of Toxoplasma gondii and Neospora caninum infection in dogs with neuromuscular disorders from urban areas in Argentina
María Laura Gos, María Cecilia Venturini, Lorena De Felice, Andrea Dellarupe, Magdalena Rambeaud, Lais Pardini, Lucía María Campero, Mariana Bernstein, Diana Bacigalupe, Walter Basso, Gastón Moré, Juan Manuel Unzaga
Veterinary Parasitology.2024; 330: 110235. CrossRef
Seroprevalence and risk factors for Toxoplasma gondii infection in shelter cats in Erzurum province of Turkey
Başak HANEDAN, Cahit BABÜR, Muhammed Sertaç EROĞLU, Selin Sinem SÜMBÜL, Ömer ALKAN
Etlik Veteriner Mikrobiyoloji Dergisi.2023; 34(2): 151. CrossRef
Prevalence of Toxoplasma gondii Measured by Western Blot, ELISA and DNA Analysis, by PCR, in Cats of Western Mexico
María de la Luz Galván-Ramírez, Claudia Charles-Niño, César Pedroza-Roldán, Carolina Salazar-Reveles, Karen Lissete Ocampo-Figueroa, Laura Roció Rodríguez-Pérez, Varinia Margarita Paez-Magallán
Pathogens.2022; 11(1): 109. CrossRef
Seroprevalence of Toxoplasma gondii in outdoor dogs and cats in Bangkok, Thailand
Ana Huertas-López, Woraporn Sukhumavasi, Gema Álvarez-García, Silvia Martínez-Subiela, David Cano-Terriza, Sonia Almería, Jitender P. Dubey, Ignacio García-Bocanegra, José Joaquín Cerón, Carlos Martínez-Carrasco
Parasitology.2021; 148(7): 843. CrossRef
Genetic Analysis of Zoonotic Gastrointestinal Protozoa and Microsporidia in Shelter Cats in South Korea
Dongmi Kwak, Min-Goo Seo
Pathogens.2020; 9(11): 894. CrossRef

7,669 View
162 Download
7 Web of Science
Crossref

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study: Yousry A. Hawash, Khadiga A. Ismail, Taisir Saber, Emad M. Eed, Amany S. Khalifa, Khalaf F. Alsharif, Saleh A. Alghamdi; Korean J Parasitol 2020;58(2):129-134.
Published online April 30, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.2.129

In most developing countries, Dientamoeba fragilis infection is an obscure protozoan infection. We aimed to determine a frequency and clinical importance of D. fragilis infection in Taif, Saudi Arabia. A 1-year case control study included patients with gastrointestinal (cases, n=114) or non-gastrointestinal symptoms (controls, n=90). The fecal samples were examined with the classical parasitological methods for intestinal protozoa, and by real time PCR for D. fragilis. The infection by D. fragilis was detected in 5.8% by PCR and in 4.4% patients by microscopy. The infection was identified more in control group (n=9) than in cases (n=3); a sole infection in 11 patients and mixed with Giardia in 1 patient. The other enteric parasites detected were Blastocystis sp. (8.3%), Giardia sp. (5.3%), Cryptosporidium sp. (2.9%), Entamoeba histolytica (1.4%), Entamoeba coli (0.9%) and Hymenolepis nana (0.4%). Our results tend to reinforce the need to increase awareness of D. fragilis infection in Saudi Arabia.

Citations

Citations to this article as recorded by

Traitement des parasitoses intestinales (amœbose exclue)
C. Prieux-Klotz, M.-C. Sabate Ferris, L. Gan, X. Bohand, P. Rey
EMC - Gastro-entérologie.2024; 41(2): 1. CrossRef
The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings
Erdogan MALATYALİ, İbrahim YILDIZ, Evren TİLEKLİOGLU, Hatice ERTABAKLAR, Sema ERTUĞ
Journal of Health Sciences and Medicine.2021; 4(4): 422. CrossRef
The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia
Adedolapo Aminat Rauff-Adedotun, Farah Haziqah Meor Termizi, Nurshafarina Shaari, Ii Li Lee
Biology.2021; 10(10): 990. CrossRef

10,564 View
169 Download
2 Web of Science
Crossref

Brief Communication

Mitochondrial Genome Sequence of Echinostoma revolutum from Red-Crowned Crane (Grus japonensis): Rongkun Ran, Qi Zhao, Asmaa M. I. Abuzeid, Yue Huang, Yunqiu Liu, Yongxiang Sun, Long He, Xiu Li, Jumei Liu, Guoqing Li; Korean J Parasitol 2020;58(1):73-79.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.73

Abstract
PDF

Echinostoma revolutum is a zoonotic food-borne intestinal trematode that can cause intestinal bleeding, enteritis, and diarrhea in human and birds. To identify a suspected E. revolutum trematode from a red-crowned crane (Grus japonensis) and to reveal the genetic characteristics of its mitochondrial (mt) genome, the internal transcribed spacer (ITS) and complete mt genome sequence of this trematode were amplified. The results identified the trematode as E. revolutum. Its entire mt genome sequence was 15,714 bp in length, including 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and one non-coding region (NCR), with 61.73% A+T base content and a significant AT preference. The length of the 22 tRNA genes ranged from 59 bp to 70 bp, and their secondary structure showed the typical cloverleaf and D-loop structure. The length of the large subunit of rRNA (rrnL) and the small subunit of rRNA (rrnS) gene was 1,011 bp and 742 bp, respectively. Phylogenetic trees showed that E. revolutum and E. miyagawai clustered together, belonging to Echinostomatidae with Hypoderaeum conoideum. This study may enrich the mitochondrial gene database of Echinostoma trematodes and provide valuable data for studying the molecular identification and phylogeny of some digenean trematodes.

Citations

Citations to this article as recorded by

The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
Biology.2025; 14(8): 1101. CrossRef
Phylogeny and morphology of some Palearctic echinostomatid digeneans from rallid bird Fulica atra Linnaeus, 1758
Sergei A. Vlasenkov, Mikhail Yu. Shchelkanov, Sergey G. Sokolov
Biologia.2025; 80(11): 3079. CrossRef
The complete mitochondrial genome of Aspidogaster ijimai (Platyhelminthes: Trematoda: Aspidogastrea): gene content and phylogenetic inference
D. A. Solodovnik, D. M. Atopkin, A. A. Semenchenko, M. Urabe, S. G. Sokolov
Invertebrate Zoology.2025; 22(3): 411. CrossRef
Molecular and morphological support for the synonymy of Nephrostomum and Patagifer: discovery of new species and broad geographic connections
María G. Díaz González, Danimar López-Hernández, Vasyl V. Tkach, Fabiana Drago, Fred D. Chibwana, Martina R. Laidemitt, Christopher A. Blanar, Verónica Núñez, Mariano Dueñas Díaz, Luis A. Gomez-Puerta, Sean A. Locke
International Journal for Parasitology.2025;[Epub] CrossRef
First molecular insights into gastrointestinal helminths of domestic birds in the Caspian Sea Littoral of Iran with an emphasis on the One Health concern
Aida Vafae Eslahi, Majid Pirestani, Armin Aligolzadeh, Oskar Nowak, Elham Hajialilo, Pourya Fathollahzadeh, Fahimeh Hajiaghaee, Milad Badri, Luís Manuel Madeira de Carvalho
Veterinary Parasitology: Regional Studies and Reports.2024; 51: 101035. CrossRef
Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples
Wasin Panich, Phonkawin Jaruboonyakorn, Awika Raksaman, Thanawan Tejangkura, Thapana Chontananarth
International Journal of Food Microbiology.2024; 418: 110732. CrossRef
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
D. M. Atopkin, A. A. Semenchenko, D. A. Solodovnik, Y. I. Ivashko
Journal of Helminthology.2023;[Epub] CrossRef
Fecal DNA metabarcoding reveals the dietary composition of wintering Red-crowned Cranes (Grus japonensis)
Hongyi Liu, Wei Xu, Nan Xu, Wenwen Zhang, Haoming Jiang, Yongqiang Zhao, Changhu Lu, Ying Zhu, Peng Xu
Avian Research.2023; 14: 100145. CrossRef
Mitophylogenomics of the zoonotic fluke Echinostoma malayanum confirms it as a member of the genus Artyfechinostomum Lane, 1915 and illustrates the complexity of Echinostomatidae systematics
Linh Thi Khanh Pham, Weerachai Saijuntha, Scott P. Lawton, Thanh Hoa Le
Parasitology Research.2022; 121(3): 899. CrossRef
Characterization of complete mitochondrial genome and ribosomal operon forCarassotrema koreanumPark, 1938 (Digenea: Haploporidae) by means of next-generation sequencing data
Y.I. Ivashko, A.A. Semenchenko, D.A. Solodovnik, D.M. Atopkin
Journal of Helminthology.2022;[Epub] CrossRef
The Ponto-Caspian parasite Plagioporus cf. skrjabini reaches the River Rhine system in Central Europe: higher infestation in the native than in the introduced Danubian form of the gastropod Theodoxus fluviatilis
Louisa Marie Rothmeier, René Sahm, Burkard Watermann, Karsten Grabow, Meike Koester, Anna Cichy, Andreas Martens
Hydrobiologia.2021; 848(10): 2569. CrossRef
Occurrence of echinostomatoids (Platyhelminthes: Digenea) in Great Cormorant (Phalacrocorax carbo) and Grey Heron (Ardea cinerea): first insights into the DNA barcodes from Lake Victoria, Tanzania
Fred Chibwana, Jestina Katandukila
African Zoology.2021; 56(3): 181. CrossRef
The mitochondrial genome sequence analysis of Ophidascaris baylisi from the Burmese python (Python molurus bivittatus)
Qi Zhao, Asmaa M.I. Abuzeid, Long He, Tingting Zhuang, Xiu Li, Jumei Liu, Shilan Zhu, Xiaoyu Chen, Guoqing Li
Parasitology International.2021; 85: 102434. CrossRef

7,068 View
120 Download
12 Web of Science
Crossref

Case Report

Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea: Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2019;57(4):417-422.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.417

Abstract
PDF

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

Citations

Citations to this article as recorded by

Exploring bioactive molecules released during inter- and intraspecific competition: A paradigm for novel antiparasitic drug discovery and design for human use
Pichet Ruenchit
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100256. CrossRef
Taeniasis impacts human gut microbiome composition and function
Wenjie Mu, Pingping Ma, Yugui Wang, Yaqi Li, Yingying Ding, Yang Zou, Lixia Pu, Qi Yan, Haoyue Kong, Xiaola Guo, Aijiang Guo, Hailong Li, Shuai Wang
The ISME Journal.2024;[Epub] CrossRef
Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China
Xiu-Min Han, Xue-Yong Zhang, Ying-Na Jian, Qing-Shan Tian
The Korean Journal of Parasitology.2021; 59(3): 311. CrossRef

11,888 View
239 Download
4 Web of Science
Crossref

Original Articles

Malaria Prevalence in a Low Transmission Area, Jazan District of Southwestern Saudi Arabia: Yousry Hawash, Khadiga Ismail, Khalaf Alsharif, Walaa Alsanie; Korean J Parasitol 2019;57(3):233-242.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.233

Abstract
PDF

Detailed description of malaria in low transmission areas is crucial for elimination. The current study aimed to provide a comprehensive description for malaria transmission in Jazan, a low transmission district, southwestern Saudi Arabia. Patients at a tertiary care hospital were recruited in our study between August 2016 and September 2018. Malaria diagnosis was performed through a species-specific nested polymerase chain reaction (nested PCR), microscopy and Paramax-3^TM rapid detection test (RDT). Malaria was detected in 30 patients by the PCR, with point prevalence of 10.9%. Of these malaria infections, 80% was imported, 26.6% was asymptomatic and 23.3% was sub-microscopic. Malaria was reported throughout the year, with February/March and September/October peaks. Infection was significantly more in males than in females (P=0.01). Likewise, infections were detected more in febrile than in non-febrile patients (P=0.01). Adult aged 15-24 years, fever and travel were identified as high-risk factors. Malaria was primarily attributed to Plasmodium falciparum mono-infections, followed by P. vivax mono-infections and lastly to falciparum/vivax mixed infections accounting 76.6%, 16.6%, and 6.6% of PCR-confirmed malaria cases, respectively. The nested PCR was superior to the smear microscopy (sensitivity 76.6%; specificity 100%) and the RDT (sensitivity 83.3%, specificity 94.2%). The overall percent agreement between microscopy and the RDT was 92.7% (kappa=0.63). High proportion of imported malaria including sub-microscopic and sub-patent cases were described. We suggest that incorporation of molecular tool into the conventional malaria diagnosis is beneficial in Jazan district.

Citations

Citations to this article as recorded by

A study on the prevalence of the malaria parasite in the city of Sebha among expatriate workers from neighboring countries.
Naima I. Al-Haddad , Rugaia M. Elsalem
Libyan Journal of Science &Technology.2025; 15(1): 186. CrossRef
Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy
Ahmed A. Muyidi, Musa A. Ayashi, Majed H. Wakid, Maimonah S. Alghanmi, Fadi M. Baakdah, Hattan S. Gattan, Isra M. Alsaady, Muslimah N. Alsulami, Haleema H. Albohiri, Sarah A. Altwaim, Zaki M. Eisa, Thamer M. Brek, José F. Silveira
Journal of Parasitology Research.2025;[Epub] CrossRef
Asymptomatic/submicroscopic Plasmodium vivax infection: A systematic review and META-analysis on the hidden challenge for preventing re-establishment of malaria transmission
Siqi Wang, He Yan, Li Zhang, Zhigui Xia, Jianhai Yin
Parasite Epidemiology and Control.2025; 30: e00442. CrossRef
The burden, clinical outcomes and risk factors related to neglected tropical diseases and malaria in migrant populations in the Middle East and North Africa: a systematic review and meta-analyses
Eman Elafef, Taha Maatoug, Stella Evangelidou, Helena Marti-Soler, Asad Adam, Ahmed Hamed Arisha, Mahmoud Hilali, Sally Hargreaves, Ibrahim Bani, Farah Seedat, Ana Requena-Méndez
BMJ Global Health.2025; 10(11): e019713. CrossRef
Spatiotemporal Distribution of Malaria in the Kingdom of Saudi Arabia
Ahmed Elagali, Mosa Shubayr, Elsiddig Noureldin, Kefyalew Addis Alene, Asmaa Elagali
Tropical Medicine and Infectious Disease.2024; 9(1): 16. CrossRef
Detection of Duffy blood group genotypes and submicroscopic Plasmodium infections using molecular diagnostic assays in febrile malaria patients
Beka R. Abagero, Rei Rama, Abdulghani Obeid, Tirusew Tolosa, Biniyam Lukas, Taye Teka, Daniel Tesfaye, Eugenia Lo, Delenasaw Yewhalaw
Malaria Journal.2024;[Epub] CrossRef
Status of Malaria Infection in Saudi Arabia
Amani Alhejely, V. Trukhachev, A. Skuratov, A. Shitikova, R. Migunov, R.Z. Abbas
BIO Web of Conferences.2024; 139: 06036. CrossRef
Machine learning models for predicting residual malaria infections using environmental factors: A case study of the Jazan region, Kingdom of Saudi Arabia
Idris Zubairu Sadiq, Yakubu Saddeeq Abubakar, Abdulkadir Rabiu Salisu, Babangida Sanusi Katsayal, Umar Saidu, Sani I. Abba, Abdullahi Garba Usman
Decoding Infection and Transmission.2024; 2: 100022. CrossRef
Investigation of Wright Blood Group Alleles and Genotypes in Malaria-Endemic Area in Southwestern Saudi Arabia
Amr Halawani, Saif Abdalla, Mahmoud Habibullah, Ghalia Shamlan, Neil Avent
International Journal of General Medicine.2024; Volume 17: 5175. CrossRef
Knowledge, attitudes, and practices (KAP) during the malaria elimination phase: A household-based cross-sectional survey
Siddig Ibrahim Abdelwahab, Ibrahim M. Elhassan, Osama Albasheer, Manal Mohamed Elhassan Taha, Nasir Ahmed Ali, Yahya Salem Al-Jabiri, Waleed Madkhali, Ahmad A. Sahly, Bassem Oraibi, Ahmed Abdallah Ahmed Altraifi, Nasser Hakami, Mohammed M. Alshehri, Moham
Medicine.2023; 102(22): e33793. CrossRef
Association between interleukin-27 gene polymorphisms and Plasmodium falciparum Malaria
Nada H. Aljarba, Mashael R. Al-Anazi, Tahani M. Al-Hazani, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Saad M. Bin Dajem, Hani Alothaid, Jahad Alghamdi, Saad Alkahtani, Ahmed A. Al-Qahtani
Innate Immunity.2023; 29(5): 83. CrossRef
Prevalence and proportion estimate of asymptomatic Plasmodium infection in Asia: a systematic review and meta-analysis
Manas Kotepui, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Aongart Mahittikorn, Polrat Wilairatana
Scientific Reports.2023;[Epub] CrossRef
A decade of travel-associated malaria at King Fahad Hospital of the University in the Eastern Province of Saudi Arabia
Ashwaq Alanazi, Bayan Ahmad Hasan Almusailhi, Gheed K. Bamousa, Nabaa H. Alhawashim, Nourah M. Alotaibi, Sumiyah AlShamekh, Basavaraja Channabasappa Hunasemarada, Reem Y. Al Jindan, Ayman A. El-Badry
Scientific Reports.2022;[Epub] CrossRef
Malarial Epidemiology among Patients in Al-Medina Al-Munawara, KSA
Shady Mohammad Raji Al-Hussayni , Sami Oudah Eid Al-Harbi , Omar Hassan Amer
International Journal of Life Science and Pharma Research.2022;[Epub] CrossRef
Comparison of Rapid Diagnostic Test, Microscopy, and Polymerase Chain Reaction for the Detection of Plasmodium falciparum Malaria in a Low-Transmission Area, Jazan Region, Southwestern Saudi Arabia
Aymen M. Madkhali, Ahmad Hassn Ghzwani, Hesham M. Al-Mekhlafi
Diagnostics.2022; 12(6): 1485. CrossRef
Genetic Diversity and Population Genetic Analysis of Plasmodium falciparum Thrombospondin Related Anonymous Protein (TRAP) in Clinical Samples from Saudi Arabia
Saad M. Bin Dajem, Md Atique Ahmed, Fatimah F. Alghnnam, Shouq F. Alghannam, Gauspasha Yusuf Deshmukh, Rehan Haider Zaidi, Marie Fe F. Bohol, Syeda Sabiha Salam, Syeda Wasfeea Wazid, Mohammed I. Shafeai, Fuad H. Rudiny, Ali M. Motaen, Kareem Morsy, Ahmed
Genes.2022; 13(7): 1149. CrossRef
Low density Plasmodium infections and G6PD deficiency among malaria suspected febrile individuals in Ethiopia
Beka R. Abagero, Daniel Kepple, Kareen Pestana, Logan Witherspoon, Abdissa Biruksew, Abinet Adanew, Fetiya Baharu, Shantoy Hansel, Karen Lopez, Daniel A. Janies, Eugenia Lo, Delenasaw Yewhalaw
Frontiers in Tropical Diseases.2022;[Epub] CrossRef
Current Status and the Epidemiology of Malaria in the Middle East Region and Beyond
Mohammad Al-Awadhi, Suhail Ahmad, Jamshaid Iqbal
Microorganisms.2021; 9(2): 338. CrossRef
Malaria Rapid Diagnostic Tests: Literary Review and Recommendation for a Quality Assurance, Quality Control Algorithm
Michael J. Kavanaugh, Steven E. Azzam, David M. Rockabrand
Diagnostics.2021; 11(5): 768. CrossRef
Current Epidemiological Characteristics of Imported Malaria, Vector Control Status and Malaria Elimination Prospects in the Gulf Cooperation Council (GCC) Countries
Jamshaid Iqbal, Suhail Ahmad, Ali Sher, Mohammad Al-Awadhi
Microorganisms.2021; 9(7): 1431. CrossRef
Residual malaria in Jazan region, southwestern Saudi Arabia: the situation, challenges and climatic drivers of autochthonous malaria
Hesham M. Al-Mekhlafi, Aymen M. Madkhali, Khalid Y. Ghailan, Ahmed A. Abdulhaq, Ahmad Hassn Ghzwani, Khalid Ammash Zain, Wahib M. Atroosh, Alkhansa Alshabi, Hussein A. Khadashi, Majid A. Darraj, Zaki M. Eisa
Malaria Journal.2021;[Epub] CrossRef
Case Report: Case report: Mixed infection of Plasmodium vivax and Plasmodium falciparum in a tertiary hospital
Abeer M. Al-Subaie
F1000Research.2021; 10: 779. CrossRef
The global burden of Plasmodium vivax malaria is obscure and insidious
Katherine E. Battle, J. Kevin Baird, Lorenz von Seidlein
PLOS Medicine.2021; 18(10): e1003799. CrossRef
The Distribution of Malaria with Seasonal in Kokap Yogyakarta 2012-2017
Raden A.K. Lestari, Novyan Lusiyana, Fitria S. Nurochmah, G. Gholib, A. Sutriana, A. Engelhardt, J. Duboscq, R. Sahara Zamzami
E3S Web of Conferences.2020; 151: 01018. CrossRef
Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
Yiu‐Wing Kam, Mohamed Yousif Ahmed, Siti Naqiah Amrun, Bernett Lee, Tarik Refaie, Kamla Elgizouli, Siew‐Wai Fong, Laurent Renia, Lisa FP Ng
Clinical & Translational Immunology.2020;[Epub] CrossRef
Rapid diagnostic tests forPlasmodium vivaxmalaria in endemic countries
Ridhi Agarwal, Leslie Choi, Samuel Johnson, Yemisi Takwoingi
Cochrane Database of Systematic Reviews.2020;[Epub] CrossRef
Decreasing trend of imported malaria cases but increasing influx of mixed P. falciparum and P. vivax infections in malaria-free Kuwait
Jamshaid Iqbal, Mohammad Al-Awadhi, Suhail Ahmad, Luzia Helena Carvalho
PLOS ONE.2020; 15(12): e0243617. CrossRef
Preparedness against self-infection and importation of Malaria - An airport survey among Saudis traveling to endemic countries
HaninSaleh AlOwaini, RizwanSuliankatchi Abdulkader, QusayFahad Almahmoud, AbdulazizEyad Alqudaimi, RaneemSaied Al-Ghamdi, WateenAhmed Alhamoud, Hasan Keriri
Journal of Family Medicine and Primary Care.2019; 8(10): 3318. CrossRef

10,277 View
171 Download
23 Web of Science
Crossref

Sinuolinea capsularis (Myxosporea: Sinuolineidae) Isolated from Urinary Bladder of Cultured Olive Flounder Paralichthys olivaceus: Sang Phil Shin, Chang Nam Jin, Han Chang Sohn, Jehee Lee; Korean J Parasitol 2019;57(2):127-134.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.127

Abstract
PDF

Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient morphological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary bladder of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were observed under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring 13.9±0.6 μm in length and 13.8±0.8 μm in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of 4.3±0.4 μm. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsularis. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.

Citations

Citations to this article as recorded by

Kudoa ogawai (Myxosporea: Kudoidae) Infection in Cultured Olive Flounder Paralichthys olivaceus
Sang Phil Shin, Chang Nam Jin, Han Chang Sohn, Jehee Lee
The Korean Journal of Parasitology.2019; 57(4): 439. CrossRef
A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea
Sang Phil Shin, Chang Nam Jin, Han Chang Sohn, Hiroshi Yokoyama, Jehee Lee
Parasitology Research.2019; 118(11): 3105. CrossRef

6,997 View
117 Download
4 Web of Science
Crossref

Brief Communication

Comparison of Two PCR Assays for Trichomonas vaginalis: Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu; Korean J Parasitol 2019;57(1):27-31.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.27

Abstract
PDF

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

Citations

Citations to this article as recorded by

Trikomoniyaz tanısında farklı laboratuvar yöntemlerinin kullanılması ve Trikomoniyaz hastalarında miRNA profilinin belirlenmesi
Hasan Turgut, Fadime Eroğlu
Dicle Tıp Dergisi.2024; 51(1): 89. CrossRef
Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases
Jie Li, Juan Du, Shengzhican Li, Jiali Dong, Jiahan Ying, Yuehao Gu, Jie Lu, Xinyu Zeng, Philip Kear, Daolong Dou, Xiaodan Wang
Phytopathology Research.2023;[Epub] CrossRef
A microfluidic-chip-based system with loop-mediated isothermal amplification for rapid and parallel detection of Trichomonas vaginalis and human papillomavirus
Zeyin Mao, Anni Deng, Xiangyu Jin, Meng Li, Wenqi Lv, Leyang Huang, Hao Zhong, Han Yang, Shihong Wang, Yixuan Shi, Lei Zhang, Qinping Liao, Guoliang Huang
The Analyst.2023; 148(19): 4820. CrossRef
Development of Nested Polymerase Chain Reaction with Novel Specific Primers for Detection of Tritrichomonas muris Infection in Laboratory Mice
Hongbo Zhang, Nan Zhang, Jianhua Li, Panpan Zhao, Xin Li, Xiaocen Wang, Xu Zhang, Bao Yuan, Fei Gao, Pengtao Gong, Xichen Zhang
Animals.2023; 13(20): 3177. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA
S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady
Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef

7,755 View
146 Download
5 Web of Science
Crossref

Case Report

An Outbreak of Histomoniasis in Backyard Sanhuang Chickens: Dandan Liu, Lingming Kong, Jianping Tao, Jinjun Xu; Korean J Parasitol 2018;56(6):597-602.
Published online December 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.6.597

Abstract
PDF

Histomonas meleagridis is a facultative anaerobic parasite, which can cause a common poultry disease known as histomoniasis. The species and age of the birds impacts on the susceptibility, with turkey being the most susceptible species. Chickens are less susceptible to H. meleagridis than turkeys and usually serve as reservoir hosts. Here, the diagnosis of an outbreak of histomoniasis in backyard Sanhuang chickens is described. The primary diagnosis was made based on clinical symptoms, general changes at necropsy, histopathology, and the isolation and cultivation of parasites. The pathogen was further confirmed by cloning, PCR identification, and animal inoculation tests. A strain of H. meleagri- dis, named HM-JSYZ-C, with a higher pathogenicity level in chickens was obtained. The study lays a foundation for further investigations into H. meleagridis and histomoniasis in chickens.

Citations

Citations to this article as recorded by

MicroRNA expression profile of chicken liver at different times after Histomonas meleagridis infection
Qiaoguang Chen, Yuming Zhang, Jie Rong, Chen Chen, Shuang Wang, Jiege Wang, Zaifan Li, Zhaofeng Hou, Dandan Liu, Jianping Tao, Jinjun Xu
Veterinary Parasitology.2024; 329: 110200. CrossRef
Molecular Detection and Characterization of Histomonas Meleagridis in Fighting Cocks, Thailand
Mangsatabam Norjit Singh, Sawang Kesdangsakonwut, Wanarit Jitsamai, Chongtham Sonia, Cherdpong Phupolpan, Piyanan Taweethavonsawat
SSRN Electronic Journal.2022;[Epub] CrossRef
MicroRNA expression profile of chicken cecum in different stages during Histomonas meleagridis infection
Yu-Ming Zhang, Qiao-Guang Chen, Chen Chen, Shuang Wang, Zai-Fan Li, Zhao-Feng Hou, Dan-Dan Liu, Jian-Ping Tao, Jin-jun Xu
BMC Veterinary Research.2022;[Epub] CrossRef
Pathologic Characterization of Coinfection with Histomonas meleagridis, Marek's Disease Virus, and Subtype J Avian Leukosis Virus in Chickens
Ming Li, Haifeng Xiong, Hanwen Wu, Dongmei Hu, Yan Lin, Xueting Huang, Jun Wang, Kezong Qi, Hongmei Liu
Avian Diseases.2021;[Epub] CrossRef
Erratum: An Outbreak of Histomoniasis in Backyard Sanhuang Chickens

The Korean Journal of Parasitology.2019; 57(1): 75. CrossRef

12,193 View
189 Download
5 Web of Science
Crossref

Original Article

Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples: Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Shin-Hyeong Cho, Jong-Yil Chai, Eun-Hee Shin; Korean J Parasitol 2018;56(5):419-427.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.419

Abstract
PDF

This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes of traveler’s diarrhea. Three target genes were specifically and simultaneously detected by the TaqMan probe method for multiple parasitic infection cases, including Cryptosporidium oocyst wall protein for C. parvum, glutamate dehydrogenase for G. lamblia, and internal transcribed spacer 1 for C. cayetanensis. Gene product 21 for bacteriophage T4 was used as an internal control DNA target for monitoring human stool DNA amplification. TaqMan probes were prepared using 4 fluorescent dyes, FAMTM, HEXTM, Cy5TM, and CAL Fluor Red® 610 on C. parvum, G. lamblia, C. cayetanensis, and bacteriophage T4, respectively. We developed a novel primer-probe set for each parasite, a primer-probe cocktail (a mixture of primers and probes for the parasites and the internal control) for multiplex real-time PCR analysis, and a protocol for this detection method. Multiplex real-time PCR with the primer-probe cocktail successfully and specifically detected the target genes of C. parvum, G. lamblia, and C. cayetanensis in the mixed spiked human stool sample. The limit of detection for our assay was 2×10 copies for C. parvum and for C. cayetanensis, while it was 2×103 copies for G. lamblia. We propose that the multiplex real-time PCR detection method developed here is a useful method for simultaneously diagnosing the most common causative protozoa in traveler’s diarrhea.

Citations

Citations to this article as recorded by

A rapid and ultrasensitive CRISPR/Cas12a-based assay for the accurate identification of T-even type phages
Chenhang Jiang, Yang Li, Ping Yu, Mengjun Fang, Di Huang, Xiangming Fang, Zhinan Xu
Biotechnology Letters.2025;[Epub] CrossRef
Assessment of Cryptosporidium spp. Sub-Families and Giardia duodenalis Assemblages A and B in Ghanaian HIV Patients, Including Socio-Economic, Clinical, and Immunological Associations
Lynn Glyschewski, Hagen Frickmann, Fred Stephen Sarfo, Betty Roberta Norman, Albert Dompreh, Emmanuel Acheamfour-Akowuah, Martin Kofi Agyei, Shadrack Osei Asibey, Richard Boateng, Edmund Osei Kuffour, Veronica Di Cristanziano, Sven Poppert, Felix Weinreic
Infectious Disease Reports.2025; 17(5): 129. CrossRef
Evaluation of molecular-based methods for the detection and quantification of Cryptosporidium spp. in wastewater
Oumaima Hachimi, Rebecca Falender, Gabriel Davis, Rispa Vranka Wafula, Melissa Sutton, June Bancroft, Paul Cieslak, Christine Kelly, Devrim Kaya, Tyler Radniecki
Science of The Total Environment.2024; 947: 174219. CrossRef
Development of duplex real‐time PCR for quick detection of cryptosporidiosis in goats
Atul Kumar Sharma, K. Gururaj, Rama Sharma, Anjana Goel, Souvik Paul, Dinesh Kumar Sharma
Cell Biochemistry and Function.2023; 41(1): 45. CrossRef
The Importance of Endoscopy with Biopsy: Real-World Evidence of Gastrointestinal Involvement in Primary Immunodeficiency in Two Main Northern Italian Centres
Stefania Nicola, Francesco Cinetto, Stefano Della Mura, Luca Lo Sardo, Elena Saracco, Ilaria Vitali, Riccardo Scarpa, Helena Buso, Vera Bonato, Claudia Discardi, Giovanni Rolla, Carla Felice, Marcello Rattazzi, Luisa Brussino
Biomedicines.2023; 11(1): 170. CrossRef
Efficacy of a membrane concentration method combined with real-time PCR for detection of Giardia and Cryptosporidium in drinking water
Jiang Jingyi, Yao Ping, Xu Jian, Chen Jia, Mao Xujian, Li Qiong, Tu Bowen, Wang Fengming
Letters in Applied Microbiology.2023;[Epub] CrossRef
Bacteriophages: from Isolation to Application
Abdallah Abdelsattar, Alyaa Dawoud, Salsabil Makky , Rana Nofal, Ramy Aziz, Ayman El-Shibiny
Current Pharmaceutical Biotechnology.2022; 23(3): 337. CrossRef
Comparative Evaluation of Real-Time Screening PCR Assays for Giardia duodenalis and of Assays Discriminating the Assemblages A and B
Felix Weinreich, Andreas Hahn, Kirsten Alexandra Eberhardt, Simone Kann, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt
Microorganisms.2022; 10(7): 1310. CrossRef
Review on Cyclosporiasis Outbreaks and Potential Molecular Markers for Tracing Back Investigations
Junqiang Li, Feifei Xu, Md Robiul Karim, Longxian Zhang
Foodborne Pathogens and Disease.2022; 19(12): 796. CrossRef
Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
Hanbi Kim, Hee Jae Huh, Eunkyoung Park, Doo-Ryeon Chung, Minhee Kang
BioChip Journal.2021; 15(1): 14. CrossRef
Comparative Performance of Eight PCR Methods to Detect Cryptosporidium Species
Damien Costa, Louise Soulieux, Romy Razakandrainibe, Louise Basmaciyan, Gilles Gargala, Stéphane Valot, Frédéric Dalle, Loic Favennec
Pathogens.2021; 10(6): 647. CrossRef
A review on application of next-generation sequencing methods for profiling of protozoan parasites in water: Current methodologies, challenges, and perspectives
N.P. Mthethwa, I.D. Amoah, P. Reddy, F. Bux, S. Kumari
Journal of Microbiological Methods.2021; 187: 106269. CrossRef
Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples
Felix Weinreich, Andreas Hahn, Kirsten Alexandra Eberhardt, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt
Pathogens.2021; 10(9): 1131. CrossRef
Advances in Cyclosporiasis Diagnosis and Therapeutic Intervention
Junqiang Li, Zhaohui Cui, Meng Qi, Longxian Zhang
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Comparison of commercial and in-house real-time PCR platforms for 15 parasites and microsporidia in human stool samples without a gold standard
Thomas Köller, Andreas Hahn, Enkhtsetseg Altangerel, Jaco J. Verweij, Olfert Landt, Simone Kann, Denise Dekker, Jürgen May, Ulrike Loderstädt, Andreas Podbielski, Hagen Frickmann
Acta Tropica.2020; 207: 105516. CrossRef
Biochemical Markers of the Functional State of the Liver during Giardiasis
D. V. Morozenko, K. V. Gliebova, S. V. Ivannikova, O. G. Geyderikh, O. V. Shapovalova, A. V. Derevyanko
Ukraïnsʹkij žurnal medicini, bìologìï ta sportu.2019; 4(2): 149. CrossRef
Molecular epidemiology of Giardia and Cryptosporidium infections – What's new?
R.C.A. Thompson, A. Ash
Infection, Genetics and Evolution.2019; 75: 103951. CrossRef
Cyclospora cayetanensis and Cyclosporiasis: An Update
Sonia Almeria, Hediye N. Cinar, Jitender P. Dubey
Microorganisms.2019; 7(9): 317. CrossRef

10,689 View
294 Download
19 Web of Science
Crossref

Brief Communication

Differential Diagnosis of Human Sparganosis Using Multiplex PCR: Hyeong-Kyu Jeon, Kyu-Heon Kim, Woon-Mok Sohn, Keeseon S. Eom; Korean J Parasitol 2018;56(3):295-300.
Published online June 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.3.295

Abstract
PDF

Human sparganosis was diagnosed by morphological and genetic analyses in Korea. The complete mitochondrial genomes of Spirometra erinaceieuropaei and S. decipiens isolated in Korea have been recorded. Present study was performed to provide information to diagnose the etiologic agent of sparganosis by multiplex PCR using mitochondrial genome sequences of S. erinaceieuropaei and S. decipiens. In an effort to examine the differential diagnosis of spirometrid tapeworms, multiplex PCR assays were performed on plerocercoid larvae of S. erinaceieuropaei and S. decipiens. The PCR products obtained using species-specific primers were positively detected in all PCR assays on mixture of S. erinaceieuropaei and S. decipiens DNA. S. erinaceieuropaei-specific bands (239 bp and 401 bp) were obtained from all PCR assays using a mixture of S. erinaceieuropaei-specific primers (Se/Sd-1800F and Se-2018R; Se/Sd-7955F and Se-8356R) and S. erinaceieuropaei template DNA. S. decipiens-specific bands (540 bp and 644 bp) were also detected in all PCR assays containing mixtures of S. decipiens-specific primers (Se/Sd-1800F and Sd-2317R; Se/Sd-7955F and Sd-8567R) and S. decipiens template DNA. Sequence analyses on these species-specific bands revealed 100% sequence identity with homologous regions of the mtDNA sequences of S. erinaceieuropaei and S. decipiens. The multiplex PCR assay was useful for differential diagnosis of human sparganosis by detecting different sizes in species-specific bands.

Citations

Citations to this article as recorded by

Molecular, morphological and histopathological evidence of Spirometra mansoni in wild and domestic animals from Costa Rica
Irene Alvarado-Hidalgo, Josué Campos-Camacho, Yuliana Arguedas-Morales, Luis M. Romero-Vega, Alejandro Alfaro-Alarcón, Gabriela Anchia-Ureña, Laura G. Bass, Ivan Berrocal-Ávila, Isabel Hagnauer, Roberto W.I. Olivares, Alberto Solano-Barquero, Rodolfo Trau
Veterinary Parasitology: Regional Studies and Reports.2024; 51: 101030. CrossRef
Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia
Wei Liu, Tengfang Gong, Shuyu Chen, Quan Liu, Haoying Zhou, Junlin He, Yong Wu, Fen Li, Yisong Liu
Animals.2022; 12(12): 1578. CrossRef
Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba
Alexander Morales, Rebeca M. Laird-Pérez, Virginia Capó, Enrique Iglesias, Luis Fonte, Arturo Plascencia-Hernández, Enrique J. Calderón, Keeseon S. Eom, Yaxsier de Armas, Héctor R. Pérez-Gómez
Pathogens.2022; 11(12): 1468. CrossRef
An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae
Blaine A. Mathison, Sarah G. H. Sapp
ZooKeys.2021; 1069: 1. CrossRef
Large-scale survey of a neglected agent of sparganosis Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae) in wild frogs in China
Xi Zhang, Xiu Hong, Shi Nan Liu, Peng Jiang, Shu Chuan Zhao, Chuan Xi Sun, Zhong Quan Wang, Jing Cui, David Blair
PLOS Neglected Tropical Diseases.2020; 14(2): e0008019. CrossRef
African Lions and Zoonotic Diseases: Implications for Commercial Lion Farms in South Africa
Jennah Green, Catherine Jakins, Eyob Asfaw, Nicholas Bruschi, Abbie Parker, Louise de Waal, Neil D’Cruze
Animals.2020; 10(9): 1692. CrossRef

7,598 View
135 Download
7 Web of Science
Crossref

Original Articles

Diagnosis, Treatment and Clinical Features of Cutaneous Leishmaniasis in Saudi Arabia: Yousry A. Hawash, Khadiga A. Ismail, Maha M. Abdel-Wahab, Mahmoud Khalifa; Korean J Parasitol 2018;56(3):229-236.
Published online June 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.3.229

Abstract
PDF

Cutaneous leishmaniasis (CL) has been one of the most common parasitic diseases in Saudi Arabia. This study exhibits the clinical features, diagnosis, cytokine profile and treatment of CL patients in Al-Taif province. Ninety CL suspects at a tertiary care general hospital were enrolled in one-year study. Patients were interviewed, clinically-examined, and subjected to laboratory tests: skin scraping smear microscopy, OligoC-TesT commercial PCR (Coris BioConcept) and kinetoplast DNA (kDNA) PCR for Leishmania diagnosis. Interferon-gamma (RayBio; Human IFN-γ) and nitric oxide (NO) levels in patients’ sera were evaluated before treatment with sodium stibogluconate (pentostam) with 20-day intramuscular drug regimen. Positive rates of microscopy, commercial PCR and kDNA PCR were 74.4%, 95.5% and 100%, respectively. Patients came to hospital mostly in winter (45.0%). CL was frequently exhibited in Saudi patients (78.8%), male gender (70.7%), age <20 years (50.0%), rural-dwellers (75.5%) and patients with travel history (86.6%). Lesion was mostly single ulcer (93.3%), occurred in the face (67.7%). Upon pentostam treatment, 85.1% of ulcers showed rapid healing signs. Levels of IFN-γ and NO were significantly higher in the healing than the non-healing cases (P<0.001). The kDNA PCR proved more sensitive than microscopy and OligoC-TesT commercial PCR. Our results open perspectives for IFN-γ use as a biomarker predicting treatment response.

Citations

Citations to this article as recorded by

Neglected Tropical Diseases (NTDs) in Saudi Arabia: Systematic Review and Meta-analysis
Najm Z. Alshahrani, Abdullah M. Alarifi, Abdullah M. Assiri
Journal of Epidemiology and Global Health.2025;[Epub] CrossRef
Tracking of Leishmania spp. in Iranian phlebotominae, distribution and biodiversity in Jarqavieh county, central Iran
Mehdi Haddadnia, Mahsa Esmaeilifallah, Parisima Badiezadeh, Seyed Mohammad Abtahi
International Journal of Environmental Health Research.2025; 35(12): 3761. CrossRef
Epidemiology and clinical features of cutaneous leishmaniasis in Khyber Pakhtunkhwa, Pakistan
W. Iqbal, U. Iram, S. Nisar, N. Musa, A. Alam, M. R. Khan, B. Ullah, M. Ullah, I. Ali
Brazilian Journal of Biology.2024;[Epub] CrossRef
Evaluation of the biodiversity and distribution of sand flies and tracking of Leishmania major in them in central Iran
Parisima Badiezadeh, Mahsa Esmaeilifallah, Mehdi Haddadniaa, Seyed Mohammad Abtahi
International Journal of Environmental Health Research.2024; 34(9): 3245. CrossRef
Epidemiology and clinical features of parasitic disease Leishmaniasis. A case study from Pakistan
Muhaibullah khan, Sheraz Ahmad, Hamayun saeed, M. Junaid Akhtar, Amna Munir
Medical Reports.2024; 6: 100090. CrossRef
Epidemiological Mapping of Cutaneous Leishmaniasis in Saudi Arabia: An Observational Descriptive Study
Basmah Alharbi, Mawahib Ahmed
Journal of Epidemiology and Global Health.2024; 14(3): 1281. CrossRef
Dermoscopic features in relation to the type of clinical lesions in cutaneous leishmaniasis
Qasim S. Al Chalabi, Hala N. Al Salman, Anfal L. Al harbawi
Journal of the Egyptian Women's Dermatologic Society.2024; 21(3): 240. CrossRef
Leishmaniasis: Recent epidemiological studies in the Middle East
Chinyere A. Knight, David R. Harris, Shifaa O. Alshammari, Ayele Gugssa, Todd Young, Clarence M. Lee
Frontiers in Microbiology.2023;[Epub] CrossRef
Cutaneous Leishmaniasis: Associated Risk Factors and Prevention in Hubuna, Najran, Saudi Arabia
Mohammed Jamaan Alzahrani, Nahid Elfaki, Yahya Hussein Ahmed Abdalla, Mugahed Ali Alkhadher, Maha Hamed Mohamed Ali, Waled AM Ahmed
International Journal of General Medicine.2023; Volume 16: 723. CrossRef
A disfiguring neglected tropical disease sweeps war-torn Yemen: a community-based study of prevalence and risk factors of cutaneous leishmaniasis among rural communities in the western highlands
Manal A Al-Ashwal, Wahib M Atroosh, Abdulelah H Al-Adhroey, Assia A Al-Subbary, Lau Yee-Ling, Hesham M Al-Mekhlafi
Transactions of The Royal Society of Tropical Medicine and Hygiene.2023; 117(12): 823. CrossRef
Distribution, clinical features, and epidemiology of cutaneous leishmaniasis: A case study of District Haripur, KPK, Pakistan
Muhaibullah Khan, Shehzad Ghayyur, Samina yasmin
Medical Reports.2023; 2: 100025. CrossRef
Cutaneous Leishmaniasis Prevalence and Clinical Overview: A Single Center Study from Saudi Arabia, Eastern Region, Al-Ahsa
Mahdi Al-Dhafiri, Abdulmohsen Alhajri, Zahraa Ali Alwayel, Jasmine Ahmed Alturaiki, Shaima Ali Bu Izran, Fatimah Ahmed Alhammad, Ryhana Mohammed Aljumaiah
Tropical Medicine and Infectious Disease.2023; 8(12): 507. CrossRef
Distribution and epidemiological features of cutaneous leishmaniasis in Asir province, Saudi Arabia, from 2011 to 2020
Yasser Alraey
Journal of Infection and Public Health.2022; 15(7): 757. CrossRef
Molecular characterization of Leishmania species from stray dogs and human patients in Saudi Arabia
Abdullah D. Alanazi, Abdulazi S. Alouffi, Mohamed S. Alyousif, Abdulsadah A. Rahi, Magda A. Ali, Hend H. A. M. Abdullah, Fabio A. Brayner, Jairo Alfonso Mendoza-Roldan, Marcos Antonio Bezerra-Santos, Domenico Otranto
Parasitology Research.2021; 120(12): 4241. CrossRef
Clinical Diagnosis and Treatment Value of CT Three-Dimensional Imaging of Gynecological Pelvic Blood Vessels
Li Qiao, Changxiao Li, Qinde Yu, Li Ma
Journal of Medical Imaging and Health Informatics.2021; 11(6): 1743. CrossRef
Prevalence of Leishmania species among patients with cutaneous leishmaniasis in Qassim province of Saudi Arabia
Zafar Rasheed, Ahmed A. Ahmed, Tarek Salem, Mohammed S. Al-Dhubaibi, Ahmad A. Al Robaee, Abdullateef A. Alzolibani
BMC Public Health.2019;[Epub] CrossRef

9,129 View
152 Download
15 Web of Science
Crossref

Hypolobocera guayaquilensis (Decapoda: Pseudothelphusidae): A New Crab Intermediate Host of Paragonimus mexicanus in Manab? Province, Ecuador: Manuel Calvopina, Daniel Romero-Alvarez, Melina Rendon, Hidekazu Takagi, Hiromu Sugiyama; Korean J Parasitol 2018;56(2):189-194.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.189

Abstract
PDF

To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of Manab? Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in Manab? Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.

Citations

Citations to this article as recorded by

Answer to April 2024 Photo Quiz
Alfredo Maldonado-Barrueco, Sol María San José-Villar, Julio García-Rodríguez, Marina Alguacil-Guillén, Álvaro López-Janeiro, Elena Trigo-Esteban, Marta Díaz-Menéndez, Guillermo Ruiz-Carrascoso, Bobbi S. Pritt
Journal of Clinical Microbiology.2024;[Epub] CrossRef
The effect of landscape and human settlement on the genetic differentiation and presence of Paragonimus species in Mesoamerica
Patricia Landaverde-González, Julie Osgood, Carlos A. Montenegro Quiñonez, Vivian Monzón, Antonieta Rodas, Carlota Monroy
International Journal for Parasitology.2022; 52(1): 13. CrossRef
General overview of the current status of human foodborne trematodiasis
Jong-Yil Chai, Bong-Kwang Jung
Parasitology.2022; 149(10): 1262. CrossRef
Liver fluke infections by Amphimerus sp. (Digenea: Opisthorchiidae) in definitive and fish intermediate hosts in Manabí province, Ecuador
Daniel Romero-Alvarez, Gabriela Valverde-Muñoz, Manuel Calvopina, Maira Rojas, William Cevallos, Hideo Kumazawa, Hidekazu Takagi, Hiromu Sugiyama, jong-Yil Chai
PLOS Neglected Tropical Diseases.2020; 14(6): e0008286. CrossRef

9,280 View
117 Download
5 Web of Science
Crossref

Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis: Samah S. Abdel Gawad, Mousa A. M. Ismail, Naglaa F. A. Imam, Ahmed H. A. Eassa, Enas Yahia abu-Sarea; Korean J Parasitol 2018;56(2):113-119.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.113

Abstract
PDF

Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20-40 year-old group, followed by 40-60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.

Citations

Citations to this article as recorded by

Presença de Cryptosporidium spp e outros enteroparasitos com potencial patogênico em pacientes em hemodiálise: um estudo aberto e controlado
Yara Leite Adami, Nycole Abreu Gama, Flavia de Souza Cunha, Regina Helena Saramago Peralta, Jocemir Ronaldo Lugon
Brazilian Journal of Nephrology.2025;[Epub] CrossRef
Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study
Yara Leite Adami, Nycole Abreu Gama, Flavia de Souza Cunha, Regina Helena Saramago Peralta, Jocemir Ronaldo Lugon
Brazilian Journal of Nephrology.2025;[Epub] CrossRef
Investigation of Cryptosporidium spp. infection among patients with diarrhea
Bassad Al‑Aboody, Noor Baqer, Ahmed Mohammed
World Academy of Sciences Journal.2025; 7(6): 1. CrossRef
Waterborne Cryptosporidium species and Giardia duodenalis in resources of MENA: A systematic review and meta-analysis
Layla Ben Ayed, Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Panagiotis Karanis
Journal of Water and Health.2024; 22(8): 1491. CrossRef
The prevalence of Cryptosporidium spp. and other intestinal parasites in elderly patients
Filiz ORAK, Adem DOĞANER, Murat ARAL, Hulusi GÜVEN, Şermin İNAL, Büşra PEMBE
Cukurova Medical Journal.2023; 48(4): 1333. CrossRef
Detection of unusual Cryptosporidium parvum subtype in patients with gastrointestinal cancer in Egypt
Mona I. Ali, Wegdan M. Abd El Wahab, Ahmed Hassan, Una Ryan, Lihua Xiao, Waleed M. Arafa, Doaa A. Hamdy
Parasitology Research.2023; 122(2): 597. CrossRef
Case Report: Severe Diarrhea Caused by Cryptosporidium Diagnosed by Metagenome Next-Generation Sequencing in Blood
Huifang Shan, Chunyan Wei, Jingyi Zhang, Min He, Zhongwei Zhang
Infection and Drug Resistance.2023; Volume 16: 5777. CrossRef
Genetic map of the isolated Cryptosporidium parasite from children with diarrheal in the city of Mosul
Hibba Alobide, Firas Alkhashab, Hiyam Altaee
Bionatura.2023; 8(4): 1. CrossRef
Molecular study of Cryptosporidium spp. among diarrheal patients at Wasit province, Iraq
May Naji Alkhanaq, Ghadeer Thamer Al-Hadidi
Asia Pacific Journal of Molecular Biology and Biotechnology.2022; : 24. CrossRef
Molecular prevalence of Cryptosporidium isolates among Egyptian children with cancer
Samira Metwally Mohammad, Magda Ali, Sara A. Abdel-rahman, Raghda Abdelrahman Moustafa, Marwa A. Salama
Journal of Parasitic Diseases.2021; 45(3): 746. CrossRef
Autophagy - related 16 - like 1 single nucleotide gene polymorphism increases the risk and severity of Cryptosporidium parvum infection
Samar A. El-Refai, Mohamed A. Helwa, Ehab B. Rakha, Amany F. Atia
Molecular and Biochemical Parasitology.2021; 245: 111407. CrossRef
Clinical and Laboratory Diagnosis of Cryptosporidiosis among Children with Acute Gastroenteritis at a Tertiary Hospital, Cairo, Egypt
Mohammad Ashraf Abdel Wahed, Yasmine Elsayed Abdelrahman Shehab, Hanan Mahmoud Abou-Seri, Yosra Mohamed Mohsen Awad
Journal of Tropical Pediatrics.2021;[Epub] CrossRef
First report and molecular characterization of Cryptosporidium spp. in humans and animals in Khartoum state, Sudan
Kaltoum Yagoub Adam, A. A. Ismail, M. A. Masri, A. A. Gameel
Veterinary World.2019; 12(1): 183. CrossRef
Cryptosporidiosis among Hemodialysis Patients in Jordan: First Preliminary Screening Surveillance
AbdelRahman M. Zueter, Nawal S. Hijjawi, Khaled N. Hamadeneh, Maysa M. Al-Sheyab, Amal M. Hatamleh
Tropical Medicine and Infectious Disease.2019; 4(4): 131. CrossRef

10,118 View
202 Download
16 Web of Science
Crossref

An Alternative Method for Extracting Plasmodium DNA from EDTA Whole Blood for Malaria Diagnosis: Krongkaew Seesui, Kanokwan Imtawil, Phimphakon Chanetmahun, Porntip Laummaunwai, Thidarut Boonmars; Korean J Parasitol 2018;56(1):25-32.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.25

Abstract
PDF

Molecular techniques have been introduced for malaria diagnosis because they offer greater sensitivity and specificity than microscopic examinations. Therefore, DNA isolation methods have been developed for easy preparation and cost effectiveness. The present study described a simple protocol for Plasmodium DNA isolation from EDTA-whole blood. This study demonstrated that after heating infected blood samples with Tris?EDTA buffer and proteinase K solution, without isolation and purification steps, the supernatant can be used as a DNA template for amplification by PCR. The sensitivity of the extracted DNA of Plasmodium falciparum and Plasmodium vivax was separately analyzed by both PCR and semi-nested PCR (Sn-PCR). The results revealed that for PCR the limit of detection was 40 parasites/μl for P. falciparum and 35.2 parasites/μl for P. vivax, whereas for Sn-PCR the limit of detection was 1.6 parasites/μl for P. falciparum and 1.4 parasites/μl for P. vivax. This new method was then verified by DNA extraction of whole blood from 11 asymptomatic Myanmar migrant workers and analyzed by Sn-PCR. The results revealed that DNA can be extracted from all samples, and there were 2 positive samples for Plasmodium (P. falciparum and P. vivax). Therefore, the protocol can be an alternative method for DNA extraction in laboratories with limited resources and a lack of trained technicians for malaria diagnosis. In addition, this protocol can be applied for subclinical cases, and this will be helpful for epidemiology and control.

Citations

Citations to this article as recorded by

Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for Rapid Plasmodium knowlesi Diagnosis
Meng-Yee Lai, Mohd Hafizi Abdul Hamid, Jenarun Jelip, Rose Nani Mudin, Yee-Ling Lau
Tropical Medicine and Infectious Disease.2023; 8(8): 389. CrossRef
Liquid Biopsy for Promising Non-invasive Diagnostic Biomarkers in Parasitic Infections
Eylem Akdur Ozturk, Ayse Caner
Acta Parasitologica.2022; 67(1): 1. CrossRef
Comparison of six methods for Loa loa genomic DNA extraction
Roland Dieki, Elsa-Rush Eyang-Assengone, Patrice Makouloutou-Nzassi, Félicien Bangueboussa, Edouard Nsi Emvo, Jean Paul Akue, Ricardo Santos
PLOS ONE.2022; 17(3): e0265582. CrossRef
Protein abundance and folding rather than the redox state of Kelch13 determine the artemisinin susceptibility of Plasmodium falciparum
Robin Schumann, Eileen Bischoff, Severina Klaus, Sophie Möhring, Julia Flock, Sandro Keller, Kim Remans, Markus Ganter, Marcel Deponte
Redox Biology.2021; 48: 102177. CrossRef

9,578 View
228 Download
6 Web of Science
Crossref

Molecular Method Confirms Canine Leishmania Infection Detected by Serological Methods in Non-Endemic Area of Brazil: Emeline Riboldi, Flavio Carvalho, Pedro Roosevelt Torres Roma?o, Regina Bones Barcellos, Graziele Lima Bello, Raquel Rocha Ramos, Rosemari Terezinha de Oliveira, Joa?o Pessoa Arau?jo Ju?nior, Maria Lucia Rossetti, Eliane Dallegrave; Korean J Parasitol 2018;56(1):11-19.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.11

Abstract
PDF

In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of ex- pansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n = 107), Sa?o Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic (DPP^®) and ELISA EIE^® assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to con- firm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in Sa?o Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.

Citations

Citations to this article as recorded by

qPCR as a Tool for the Diagnosis of Visceral and Cutaneous Leishmaniasis: A Systematic Review and Meta-Analysis
José Bryan Rihs, Mariana Teixeira Vilela, Janete Soares Coelho dos Santos, Job Alves de Souza Filho, Sérgio Caldas, Rodrigo Souza Leite, Marcos Paulo Gomes Mol
Acta Parasitologica.2025;[Epub] CrossRef
Global Distribution of Canine Visceral Leishmaniasis and the Role of the Dog in the Epidemiology of the Disease
Diego Fernandes Vilas-Boas, Eiji Kevin Nakasone Nakasone, Ana Alice Maia Gonçalves, Daniel Ferreira Lair, Diana Souza de Oliveira, Diogo Fonseca Soares Pereira, Geralda Gabriele Silva, Ingrid dos Santos Soares Conrado, Lucilene Aparecida Resende, Maykelin
Pathogens.2024; 13(6): 455. CrossRef
High-resolution mapping of linear epitopes from LiNTPDase2: Advancing leishmaniasis detection using optimized protein and peptide antigens
Raissa Barbosa de Castro, João Victor Badaró de Moraes, Anna Cláudia Alves de Souza, Evandro Silva Favarato, Fabiana Azevedo Voorwald, Fabiane Matos dos Santos, Gustavo Costa Bressan, Raphael de Souza Vasconcellos, Juliana Lopes Rangel Fietto
Diagnostic Microbiology and Infectious Disease.2024; 110(2): 116448. CrossRef
Development and preliminary study of the rLiNTPDase2 rapid test: A lateral flow immunochromatographic assay for Canine Visceral Leishmaniasis
Raissa Barbosa de Castro, Luma Salgado Leopoldino, João Victor Badaró de Moraes, Gustavo Costa Bressan, Raphael de Souza Vasconcellos, Evandro Silva Favarato, Fabiana Azevedo Voorwald, Juliana Lopes Rangel Fietto
Veterinary Parasitology.2024; 331: 110299. CrossRef
Identification of infection by Leishmania spp. in wild and domestic animals in Brazil: a systematic review with meta-analysis (2001–2021)
Fabiana Raquel Ratzlaff, Vanessa Osmari, Daniele da Silva, Jaíne Soares de Paula Vasconcellos, Luciana Pötter, Fagner D’ambroso Fernandes, José Américo de Mello Filho, Sônia de Avila Botton, Fernanda Silveira Flores Vogel, Luís Antônio Sangioni
Parasitology Research.2023; 122(7): 1605. CrossRef
Dynamics of Leishmania spp. infection in dogs from an unaffected region in transition to a visceral leishmaniasis transmission area, Rio Grande do Sul, Brazil
Michelli Lopes de Souza, Isac Junior Roman, Ana Paula Gnocato Mortari, Fabiana Raquel Ratzlaff, Renata Dalcol Mazaro, Juliana Felipetto Cargnelutti, Irina Lübeck, Rafael Almeida Fighera, Cinthia Melazzo de Andrade, Sônia de Ávila Botton, Fernanda Silveira
Parasitology Research.2022; 121(11): 3269. CrossRef
Canine Visceral Leishmaniasis in an Area of Sporadic Transmission in Brazil
Leandro Machado Borges, Alessandra Gutierrez de Oliveira, Nathália Lopes Fontoura Mateus, Everton Falcão de Oliveira, Aline Etelvina Casaril Arrua, Jucelei de Oliveira Moura Infran, Lucas Bezerra Taketa, Paulo Eduardo de Oliveira Monteiro, Carlos Eurico d
Vector-Borne and Zoonotic Diseases.2021; 21(7): 539. CrossRef
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests
Anaiá da Paixão Sevá, Ana Pérola Drulla Brandão, Silvia Neri Godoy, Rodrigo Martins Soares, Helio Langoni, Bruna Cristine Rodrigues, Mariana Zanchetta e Gava, Paula Ferraz de Camargo Zanotto, Tatiana Jimenez-Villegas, Roberto Hiramoto, Fernando Ferreira
Revista da Sociedade Brasileira de Medicina Tropical.2021;[Epub] CrossRef
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
Laura Ramírez, Luana Dias de Moura, Natalia Lopes Fontoura Mateus, Milene Hoehr de Moraes, Leopoldo Fabrício Marçal do Nascimento, Nailson de Jesus Melo, Lucas Bezerra Taketa, Tatiana Catecati, Samuel G. Huete, Karla Penichet, Eliane Mattos Piranda, Aless
Parasite Epidemiology and Control.2020; 8: e00126. CrossRef
Canine visceral leishmaniasis in area with recent Leishmania transmission: prevalence, diagnosis, and molecular identification of the infecting species
Josiane Valadão Lopes, Érika Monteiro Michalsky, Nathália Cristina Lima Pereira, Adão Junior Viana de Paula, Andreza Geisiane Maia Souza, Letícia Cavalari Pinheiro, Ana Cristina Vianna Mariano da Rocha Lima, Daniel Moreira de Avelar, João Carlos França-Si
Revista da Sociedade Brasileira de Medicina Tropical.2020;[Epub] CrossRef
Canine visceral leishmaniasis: Detection of Leishmania spp. genome in peripheral blood of seropositive dogs by real-time polymerase chain reaction (rt-PCR)
Fabrício Moreira Monteiro, Amanda Sanchez Machado, Fabiana Rocha-Silva, Cláudia Barbosa Assunção, Cidiane Graciele-Melo, Lourena Emanuele Costa, Aquila Serbate Portela, Eduardo Antonio Ferraz Coelho, Sônia Maria de Figueiredo, Rachel Basques Caligiorne
Microbial Pathogenesis.2019; 126: 263. CrossRef
PREVALÊNCIA DA LEISHMANIOSE VISCERAL CANINA E COINFECÇÕES EM REGIÃO PERIURBANA NO DISTRITO FEDERAL – BRASIL
Cassio Ricardo Ribeiro, Carolynne Arruda Gonçalves, Lauricio Monteiro Cruz, Paula Diniz Galera
Ciência Animal Brasileira.2019;[Epub] CrossRef
Multiplex flow cytometry serology to diagnosis of canine visceral leishmaniasis
Henrique Gama Ker, Wendel Coura-Vital, Diogo Garcia Valadares, Rodrigo Dian Oliveira Aguiar-Soares, Rory Cristiane Fortes de Brito, Patrícia Sampaio Tavares Veras, Deborah Bittencourt Mothé Fraga, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Alex
Applied Microbiology and Biotechnology.2019; 103(19): 8179. CrossRef
Natural infection by Leishmania infantum in the Lutzomyia longipalpis population of an endemic coastal area to visceral leishmaniasis in Brazil is not associated with bioclimatic factors
Tiago Feitosa Mota, Orlando Marcos Farias de Sousa, Yuri de Jesus Silva, Lairton Souza Borja, Bruna Martins Macedo Leite, Manuela da Silva Solcà, Djalma Alves de Melo, Claudia Ida Brodskyn, Edelberto Santos Dias, Patrícia Sampaio Tavares Veras, Deborah Bi
PLOS Neglected Tropical Diseases.2019; 13(8): e0007626. CrossRef

10,889 View
234 Download
15 Web of Science
Crossref

First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal: Haroon Ahmed, S?rgio Ramalho Sousa, Sami Simsek, Sofia Anast?cio, Seyma Gunyakti Kilinc; Korean J Parasitol 2017;55(6):653-658.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.653

Abstract
PDF

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The
objective
of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Citations

Citations to this article as recorded by

Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia
Ema Gagović, Daria Jurković Žilić, Krunoslav Pintur, Adnan Hodžić, Šimun Naletilić, Relja Beck
Animals.2025; 15(2): 208. CrossRef
Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain)
Sara González, Rosario Panadero, María Luisa Del Rio, María Natividad Díez, María del Rosario Hidalgo, Angélica Martínez
Veterinary Research Communications.2024; 48(2): 761. CrossRef
Molecular characterization of the camel nasal botfly, Cephalopina titillator (Diptera: Oestridae)
Mona G. Shaalan, Sherif Hamed Farghaly, Emad I. Khater, Mohamed A. Kenawy, Enas Hamdy Ghallab
Beni-Suef University Journal of Basic and Applied Sciences.2024;[Epub] CrossRef
Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis
Sara González, Maria Luisa Del Rio, Maria Natividad Diez, Maria del Rosario Hidalgo, Angelica Martínez
Microscopy Research and Technique.2023; 86(1): 3. CrossRef
Temporal and spatial spread of Hypoderma actaeon infection in roe deer from peninsular Spain determined by an indirect enzyme‐linked immunosorbent assay
R. Panadero, C. M. López, S. Remesar, E. Cabanelas, G. Varas, F. Markina, P. Díaz, D. García‐Dios, A. Prieto, G. Fernández, P. Díez‐Baños, P. Morrondo
Medical and Veterinary Entomology.2020; 34(1): 44. CrossRef

8,662 View
155 Download
8 Web of Science
Crossref

Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran: Somaie Matin, Gholamreza Shahbazi, Shervin Tabrizian Namin, Rouhallah Moradpour, Farideh Feizi, Hadi Piri-dogahe; Korean J Parasitol 2017;55(6):607-612.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.607

Abstract
PDF

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.

Citations

Citations to this article as recorded by

Seroepidemiology, molecular detection, and isolation attempts of Toxoplasma gondii in dairy goats from Rio de Janeiro, Brazil
Igor Falco Arruda, Patricia Riddell Millar, Fernanda da Silva Lopes, Caroline Martins da Costa, Mario Felipe Alvarez Balaro, Lucas Almeida Zangirolami, Raissa Cristina Ferreira Ramos, Thamires Francisco Bonifácio, Julia Araujo Panza Ribeiro, Maria Regina
Veterinary Research Communications.2025;[Epub] CrossRef
Prevalence of Acute and Chronic Toxoplasma gondii Infection in the Iranian Women of Reproductive Age: A Systematic Review and Meta-Analysis of Data Published Between 2010 - 2024
Forouzan Rahmani, Shabnam Hasani, Masoumeh Sadat Mousavi, Leila Kianfard
Journal of Kermanshah University of Medical Sciences.2025;[Epub] CrossRef
Unraveling the link: serological and molecular insights into Toxoplasma gondii infection in women with spontaneous abortion history
Narges Arbabi, Nima Firouzeh, Seyed Ghader Azizi, Ahmad Mehravaran, Soudabeh Etemadi, Reza Shafiei, Hadi Mirahmadi
Tropical Diseases, Travel Medicine and Vaccines.2025;[Epub] CrossRef
IgG avidity and placenta real-time PCR in detection of active maternal toxoplasmosis: relation to pregnancy outcomes
Amal Farahat Allam, Amel Youssef Shehab, Nashwa Abdelaleem Ahmed, Amel Gaber Elshereedy, Hoda Fahmy Farag, Sarah Mohamed Abdo, Heba Said Ibrahim
Experimental Parasitology.2025; 279: 109065. CrossRef
Comparison of immunological and molecular methods for laboratory diagnosis of ocular toxoplasmosis in blood, serum and tears in Brazil
Raissa Cristina Ferreira Ramos, Alynne da Silva Barbosa, Ana Luisa Quintella do Couto Aleixo, Igor Falco Arruda, Maria Regina Reis Amendoeira, Khaled Abd EL-Hamid Abd EL-Razik
PLOS ONE.2024; 19(2): e0298393. CrossRef
Serological and molecular detection of Toxoplasma gondii in ß. thalassemia patients
Naglaa Fathi Abd El-Latif, Aziza Ibrahim Salem, Nadia Aly Sadek, Sally A. M. Salah, Doaa Gaber Shalaby, Heba Elhadad
Journal of Parasitic Diseases.2023; 47(4): 778. CrossRef
Infectious and environmental placental insults: from underlying biological pathways to diagnostics and treatments
Samuel Chenge, Harrison Ngure, Bernard N Kanoi, Amanda N Sferruzzi-Perri, Francis M Kobia
Pathogens and Disease.2023;[Epub] CrossRef
Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran
Shiva Zeinali, Shahram Khademvatan, Rasool Jafari, Shabnam Vazifekhah, Elham Yousefi, Tahereh Behroozi-Lak, Bibi Razieh Hosseini Farash
PLOS ONE.2023; 18(10): e0283493. CrossRef
Toksoplazma serolojisi pozitif gebelerde intrauterin toksoplazmoz riskinin değerlendirilmesi
Ümit GÖRKEM, Cagdaş KOCAMAN, Djursun KARASARTOVA, Deniz TAŞKIRAN, Ayşe GÜRESER, Cahit BABÜR, Nezahat KOŞAR, Ayşegül TAYLAN ÖZKAN
Avrasya Sağlık Bilimleri Dergisi.2022; 5(3): 54. CrossRef
Toxoplasma gondii-associated Placentitis in the absence of maternal seroconversion
Christen Rune Stensvold, Lone Storgaard, Lisa Leth Maroun, Jørgen Anders Lindholm Kurtzhals, Henrik Vedel Nielsen
Parasite Epidemiology and Control.2022; 19: e00279. CrossRef
Detection and genetic characterisation of Toxoplasma gondii circulating in free-range chickens, pigs and seropositive pregnant women in Benue state, Nigeria
Ifeoma N. Nzelu, Jacob K. P. Kwaga, Junaidu Kabir, Idris A. Lawal, Christy Beazley, Laura Evans, Damer P. Blake, Arnau Casanovas-Massana
PLOS Neglected Tropical Diseases.2021; 15(6): e0009458. CrossRef
Toxoplasma gondii infection and spontaneous abortion: A systematic review and meta-analysis
Narges Kalantari, Tahmineh Gorgani-Firouzjaee, Zahra Moulana, Mohammad Chehrazi, Salman Ghaffari
Microbial Pathogenesis.2021; 158: 105070. CrossRef
Genetic characterization of Toxoplasma gondii isolates from human spontaneous aborted fetuses in Jahrom, southern Iran
Salar Maani, Hassan Rezanezhad, Kavous Solhjoo, Mohsen Kalantari, Saiedeh Erfanian
Microbial Pathogenesis.2021; 161: 105217. CrossRef
Detection of Acute and Chronic Toxoplasma gondii Infection among Women with History of Abortion in the Southwest of Iran
Jasem Saki, Maryam Zamanpour, Mahin Najafian, Niloofar Mohammadpour, Masoud Foroutan, José F. Silveira
Journal of Parasitology Research.2021; 2021: 1. CrossRef
The global seroprevalence of anti-Toxoplasma gondii antibodies in women who had spontaneous abortion: A systematic review and meta-analysis
Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Afsaneh Amouei, Zahra Hosseininejad, Ahmad Daryani, Maria Angeles Gómez-Morales
PLOS Neglected Tropical Diseases.2020; 14(3): e0008103. CrossRef
Factors associated with spontaneous abortion: a systematic review
Maria Tânia Silva Oliveira, Caline Novais Teixeira Oliveira, Lucas Miranda Marques, Cláudio Lima Souza, Márcio Vasconcelos Oliveira
Revista Brasileira de Saúde Materno Infantil.2020; 20(2): 361. CrossRef
Molecular genotyping and serological evaluation of Toxoplasma gondii in mothers and their spontaneous aborted fetuses in Southwest of Iran
Nasir Arefkhah, Bahman Pourabbas, Qasem Asgari, Abdolali Moshfe, Fataneh Mikaeili, Gordafarin Nikbakht, Bahador Sarkari
Comparative Immunology, Microbiology and Infectious Diseases.2019; 66: 101342. CrossRef
Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis
Khosro Sardarian, Amir Hossein Maghsood, Marzieh Farimani, Mehrdad Hajiloii, Massoud Saidijam, Mahsa Rezaeepoor, Hanie Mahaki, Alireza Zamani
Advanced Biomedical Research.2018;[Epub] CrossRef

9,115 View
222 Download
18 Web of Science
Crossref

Genotypic Identification of Cystoisospora in Immunocompromised Patients Using T_m-Variation Analysis: Maha M.A. Basyoni, Hany Ahmed Fouad Elghobary; Korean J Parasitol 2017;55(6):601-606.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.601

Abstract
PDF

Cystoisospora is responsible for morbidity in immunocompromised patients. PCR is sensitive for diagnosing Cystoisospora; however, it needs reevaluation for differential molecular diagnosis of cystoisosporiasis. We aimed at evaluating melting curve analysis (MCA) after real-time PCR (qPCR) in diagnosis and genotyping of Cystoisospora as an alternative to conventional PCR. We included 293 diarrheic stool samples of patients attending the Department of Clinical Oncology and Nuclear Medicine of Cairo University Hospitals, Egypt. Samples were subjected to microscopy, nested PCR (nPCR), and qPCR targeting the internal transcribed spacer 2 region (ITS2) of the ribosomal RNA (r RNA) gene followed by melting temperatures (T_ms) analysis and comparing the results to PCR-RFLP banding patterns. Using microscopy and ITS2-nPCR, 3.1% and 5.8% of cases were Cystoisospora positive, respectively, while 10.9% were positive using qPCR. Genotyping of Cystoisospora by qPCR-MCA revealed 2 genotypes. These genotypes matched with 2 distinct melting peaks with specified Tm_m at 85.8˚C and 88.6˚C, which indicated genetic variation among Cystoisospora isolates in Egypt. Genotype II proved to be more prevalent (65.6%). HIV-related Kaposi sarcoma and leukemic patients harbored both genotypes with a tendency to genotype II. Genotype I was more prevalent in lymphomas and mammary gland tumors while colorectal and hepatocellular tumors harbored genotype II suggesting that this genotype might be responsible for the development of cystoisosporiasis in immunocompromised patients. Direct reliable identification and differentiation of Cystoisospora species could be established using qPCR-T_ms analysis which is useful for rapid detection and screening of Cystoisospora genotypes principally in high risk groups.

9,528 View
143 Download

Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis: Sara Nemati, Asghar Fazaeli, Homa Hajjaran, Ali Khamesipour, Mohsen Falahati Anbaran, Arezoo Bozorgomid, Fatah Zarei; Korean J Parasitol 2017;55(4):367-374.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.367

Abstract
PDF

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.

Citations

Citations to this article as recorded by

Existing and newly emerging human cutaneous Leishmania isolates in Ethiopia: a systematic review
Abiy Ayele Angelo, Getu Girmay, Dereje Mengesha Berta, Bisrat Birke Teketelew, Elias Chane, Negesse Cherie, Muluneh Assefa, Zufan Yiheyis Abriham, Mebratu Tamir
Discover Medicine.2025;[Epub] CrossRef
Update on the Seroepidemiology of Human Cystic Echinococcosis and Associated Risk Factors in Iran: A Systematic Review and Meta‐Analysis
Nashmin Mohemmi, Mohammad Taghi Khodayari, Seyed Ahmad Karamati, Mohammad Reza Shiee, Hossein Effatpanah, Afshin Davari, Ali Reza Ghorbani, Arezoo Bozorgomid
Health Science Reports.2025;[Epub] CrossRef
Genetic Diversity and Chromosomal Variations in the Iranian Leishmania major Strain: Insights into Pathogenicity and Drug Resistance
Hanieh Sharifian, Anis Khalafiyan, Mahmood Fadaie, Hossein Khanahmad, Zabihollah Shahmoradi, Erfan Zaker, Parisa Mousavi, Nadia Pourmoshir, Azadeh Zolfaghari
Advanced Biomedical Research.2025;[Epub] CrossRef
Genetic diversity and epidemiological insights into cutaneous leishmaniasis in Pakistan: a comprehensive study on clinical manifestations and molecular characterization of Leishmania species
Shumaila Naz, Muhammed Nalcaci, Obaid Hayat, Seray Toz, Azhar Minhas, Shahid Waseem, Yusuf Ozbel
Parasitology Research.2024;[Epub] CrossRef
Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran
Naser Nazari, Mohamad Bagher Rokni, Madoka Ichikawa‐Seki, Saber Raeghi, Homa Hajjaran, Shahab Falahi, Yazdan Hamzavi, Peyman Heydarian, Afshin Davari, Keyphobad Ghadiri, Arezoo Bozorgomid
Veterinary Medicine and Science.2023; 9(2): 924. CrossRef
Real‐time impact of COVID‐19 pandemic on cutaneous leishmaniasis case finding and strategic planning, preventive interventions, control and epidemiology in a region with a high burden of cutaneous leishmaniasis and COVID‐19: A cross‐sectional descriptive
Morteza Shams, Ayoub Rashidi, Jasem Mohamadi, Mohamad Moradi, Reza Pakzad, Razi Naserifar, Jahangir Abdi, Fariba Ghelichi, Arezoo Bozorgomid, Nahid Maspi, Azra Kenarkoohi, Yasin Mohammadi, Amir Abdoli, Shahab Falahi
Health Science Reports.2023;[Epub] CrossRef
An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia
Adriana C. Castillo-Castañeda, Luz H. Patiño, Maria Fernanda Zuñiga, Omar Cantillo-Barraza, Martha S. Ayala, Maryi Segura, Jessica Bautista, Plutarco Urbano, Jeiczon Jaimes-Dueñez, Juan David Ramírez
Parasites & Vectors.2022;[Epub] CrossRef
Taxonomy, Population Structure and Genetic Diversity of Iranian Leishmania Strains of Cutaneous and Visceral Leishmaniasis
Sara Nemati, Homa Hajjaran, Soudabeh Heydari, Asghar Fazaeli, Ali Khamesipour, Mohsen Falahati Anbaran, Mehdi Mohebali, Hamed Mirjalali
Acta Parasitologica.2021; 66(4): 1274. CrossRef
The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis
Homa Hajjaran, Reza Saberi, Alireza Borjian, Mahdi Fakhar, Seyed Abdollah Hosseini, Sajjad Ghodrati, Mehdi Mohebali
Frontiers in Public Health.2021;[Epub] CrossRef
Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania
Juan Jose Lauthier, Paula Ruybal, Paola Andrea Barroso, Yoshihisa Hashiguchi, Jorge Diego Marco, Masataka Korenaga
Acta Tropica.2020; 201: 105189. CrossRef
Isolation and Molecular Identification of Leishmania spp. in Patients With Cutaneous Leishmaniasis in Golestan Province, Iran
Ali Fattahi Bafghi, Gilda Eslami, Oghol Niazjorjani, Farzaneh Mirzaei, Javad Namrodi
International Journal of Epidemiologic Research.2019; 6(1): 8. CrossRef
Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks
Giovanny Herrera, Adriana Higuera, Luz Helena Patiño, Martha S. Ayala, Juan David Ramírez
Infection, Genetics and Evolution.2018; 64: 135. CrossRef
Cutaneous leishmaniasis in Qasr-e Shirin, a border area in the west of Iran
Yazdan Hamzavi, Naser Nazari, Nahid Khademi, Keivan Hassani, Arezoo Bozorgomid
Veterinary World.2018; : 1692. CrossRef

10,803 View
234 Download
13 Web of Science
Crossref

Brief Communication

PCR Identification and Phylogenetic Analysis of Trichomonas gallinae from Domestic Pigeons in Guangzhou, China: Shen-Ben Qiu, Meng-Na Lv, Xi He, Ya-Biao Weng, Shang-Shu Zou, Xin-Qiu Wang, Rui-Qing Lin; Korean J Parasitol 2017;55(3):333-336.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.333

Abstract
PDF

Avian trichomoniasis caused by Trichomonas gallinae is a serious protozoan disease worldwide. The domestic pigeon (Columba livia domestica) is the main host for T. gallinae and plays an important role in the spread of the disease. Based on the internal transcribed spacers of nuclear ribosomal DNA of this parasite, a pair of primers (TgF2/TgR2) was designed and used to develop a PCR assay for the diagnosis of T. gallinae infection in domestic pigeons. This approach allowed the identification of T. gallinae, and no amplicons were produced when using DNA from other common avian pathogens. The minimum amount of DNA detectable by the specific PCR assay developed in this study was 15 pg. Clinical samples from Guangzhou, China, were examined using this PCR assay and a standard microscopy method, and their molecular characteristics were determined by phylogenetic analysis. All of the T. gallinae-positive samples detected by microscopic examination were also detected as positive by the PCR assay. Most of the samples identified as negative by microscopic examination were detected as T. gallinae positive by the PCR assay and were confirmed by sequencing. The positive samples of T. gallinae collected from Guangzhou, China, were identified as T. gallinae genotype B by sequencing and phylogenetic analyses, providing relevant data for studying the ecology and population genetic structures of trichomonads and for the prevention and control of the diseases they cause.

Citations

Citations to this article as recorded by

Rapid and sensitive detection of Trichomonas gallinae using RAA-CRISPR-Cas12a
Yuhan Zhou, Yaqian Chen, Xinglong Song, Zhenyu Zhong, Qingyun Guo, Shengfan Jing, Olalekan Opeyemi Ayanniyi, Zhenxiao Lu, Qingxun Zhang, Congshan Yang
Veterinary Parasitology.2025; 334: 110412. CrossRef
Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for the Rapid and Sensitive Detection of Trichomonas gallinae
Yuhan Zhou, Xinglong Song, Yaqian Chen, Zhenyu Zhong, Qingyun Guo, Shengfan Jing, Yilei Zhang, Qingxun Zhang, Congshan Yang
Journal of Parasitology.2025;[Epub] CrossRef
An Evolutionarily Conserved Laterally Acquired Toolkit Enables Microbiota Targeting by Trichomonas
Adam J Hart, Lenshina A Mpeyako, Nick P Bailey, George Merces, Joseph Gray, Jacob Biboy, Manuel Banzhaf, Waldemar Vollmer, Robert P Hirt, Ana Carolina Junqueira
Molecular Biology and Evolution.2025;[Epub] CrossRef
Genotypic diversity and epidemiology of Trichomonas gallinae in Columbidae: Insights from a comprehensive analysis
Yu Liu, Haiming Cai, Dingai Wang, Shenquan Liao, Nanshan Qi, Juan Li, Zhuanqiang Yan, Hanqin Shen, Siyun Fang, Minna Lv, Xuhui Lin, Yongle Song, Junjing Hu, Yibin Zhu, Xiangjie Chen, Lijun Yin, Jianfei Zhang, Yaqiong Guo, Mingfei Sun
International Journal for Parasitology: Parasites and Wildlife.2024; 23: 100918. CrossRef
Prevalence and molecular characterization of Trichomonas gallinae from pigeons in Anhui, China
Yilei Zhang, Zhenxiao Lu, Zhenzhen Liu, Yuhan Zhou, Guodong Xiao, Ayanniyi Olalekan Opeyemi, Sihua Jin, Yongsheng Li, Tao Liu, Qiong Wu, Xingdong Sun, Qianming Xu, Qingxun Zhang, Congshan Yang
Comparative Immunology, Microbiology and Infectious Diseases.2024; 107: 102157. CrossRef
SURVEY ON THE MOST COMMON DISEASES CIRCULATING AMONG PIGEONS IN THE EASTERN PROVINCE, SAUDI ARABIA
Mahmoud H.A.Mohamad, Adel M.Abdel Aziz
The Journal of Animal and Plant Sciences.2023; (5): 1220. CrossRef
Genetic characterization of Trichomonas gallinae (Rivolta, 1878) in companion birds in Japan and the genotypical relationship in the Asia region
Shyun Chou, Shinichiro Hadano, Atsushi Kojima, Mario Yorisaki, Masaru Yasuda, Kazunori Ike, Toshihiro Tokiwa
Journal of Microbiology, Immunology and Infection.2022; 55(3): 527. CrossRef
A new duplex real-time PCR for simultaneous detection and differentiation of Tetratrichomonas gallinarum and Trichomonas gallinae
Brigitte Sigrist, T. W. Cherry Ng, Sarah Albini, Nina Wolfrum
Journal of Veterinary Diagnostic Investigation.2022; 34(4): 631. CrossRef
Molecular prevalence of Tetratrichomonas gallinarum and Trichomonas gallinae in three domestic free-range poultry breeds in Anhui Province, China
Dong-qian Chen, Xin-yu Luo, Qiao-qiao Li, Jin-chao Pan, Hen Zhang, Yue-yue Gu, Zhen-zhen Kan, Jia-min Huang, Zhui Fang, Xin-chao Liu, You-fang Gu, Wen-Chao Li
Parasitology Research.2022; 121(10): 2841. CrossRef
Prevalence and diversity of Trichomonas gallinae in meat pigeons (Columba livia) in Guangdong Province, People’s Republic of China
Haiming Cai, Yu Liu, Yibin Zhu, Zhihong Xu, Qingfeng Zhou, Zhuanqiang Yan, Shenquan Liao, Nanshan Qi, Juan Li, Xuhui Lin, Junjing Hu, Shuilan Yu, Jianfei Zhang, Junwei Lin, Minna Lv, Mingfei Sun
Parasitology Research.2022; 121(11): 3183. CrossRef
Common bacterial, viral, and parasitic diseases in pigeons (Columba livia): A review of diagnostic and treatment strategies
Harvey M. Santos, Ching-Yi Tsai, Gail Everette M. Catulin, Kim Chloe G. Trangia, Lemmuel L. Tayo, Hung-Jen Liu, Kuo Pin Chuang
Veterinary Microbiology.2020; 247: 108779. CrossRef
Prevalence and molecular characterization of Trichomonas gallinae from domestic pigeons in Beijing, China
Sheng-Yong Feng, Han Chang, Fu-Huang Li, Cheng-Min Wang, Jing Luo, Hong-Xuan He
Infection, Genetics and Evolution.2018; 65: 369. CrossRef

8,283 View
169 Download
13 Web of Science
Crossref

Original Articles

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran: Meysam Sharifdini, Zahra Heidari, Zahra Hesari, Sajad Vatandoost, Eshrat Beigom Kia; Korean J Parasitol 2017;55(3):279-285.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.279

Abstract
PDF

The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

Citations

Citations to this article as recorded by

Helminth Infections in Cattle: A Study on Fecal Samples from the Ağrı Region
Milad Afşar, Selahattin Aydemir, Sedat Kavak, Sadi Elasan
Van Veterinary Journal.2025; 36(2): 95. CrossRef
Molecular identification of abomasal nematodes (Haemonchus spp. and Ostertagia ostertagi) in naturally infected calves
H. H. Shihab, S. D. Hassan
BULGARIAN JOURNAL OF VETERINARY MEDICINE.2024; 27(2): 286. CrossRef
Trichostrongylosis: a zoonotic disease of small ruminants
A.H. Bhat, H. Tak, I.M. Malik, B.A. Ganai, N. Zehbi
Journal of Helminthology.2023;[Epub] CrossRef
Prevalence and Morphometric Comparison of Trichostrongylus spp. among Sheep and Goats from Kashan Abattoir, Central Iran
Mohsen Arbabi, Aimohammad Bakhshi, Hossein Hooshyar, Reza Ghasemikhah, Mahdi Delavari, Mojtaba Sehat
Journal of Medical Microbiology and Infectious Diseases.2023; 11(1): 28. CrossRef
Investigating intestinal parasitic infections with emphasis on molecular identification of Strongyloides stercoralis and Trichostrongylus colubriformis in north of Iran
Fatemeh Hajizadeh, Tahereh Mikaeili Galeh, Seyed Abdollah Hosseini, Seyyed Ali Shariatzadeh, Akram Hematizadeh, Javad Javidnia, Mitra Sadeghi, Mahdi Fakhar, Shirzad Gholami
Parasite Epidemiology and Control.2023; 22: e00312. CrossRef
Molecular identification of different Trichostrongylus species infecting sheep and goats from Dakahlia governorate, Egypt
Rana Elseadawy, Ibrahim Abbas, Moustafa Al-Araby, Salah Abu-Elwafa
Journal of Parasitic Diseases.2021; 45(1): 218. CrossRef
Strongyloides stercoralis and other intestinal parasites in patients receiving immunosuppressive drugs in northern Iran: a closer look at risk factors
Leila Mirzaei, Keyhan Ashrafi, Zahra Atrkar Roushan, Mohammad Reza Mahmoudi, Irandokht Shenavar Masooleh, Behnaz Rahmati, Farshid Saadat, Hamed Mirjalali, Meysam Sharifdini
Epidemiology and Health.2021; 43: e2021009. CrossRef
Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis
Mehdi Pandi, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Behnaz Rahmati, Nayereh Hajipour
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
Hedayat Hosseinnezhad, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Hamed Mirjalali, Behnaz Rahmati
BMC Veterinary Research.2021;[Epub] CrossRef
Prevalence and risk factors of intestinal parasite infections in Mazandaran province, North of Iran
Zeinab Gholipoor, Hooshang Khazan, Eznoallah Azargashb, Mohammad Reza Youssefi, Ali Rostami
Clinical Epidemiology and Global Health.2020; 8(1): 17. CrossRef
Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations
Keyhan Ashrafi, Meysam Sharifdini, Zahra Heidari, Behnaz Rahmati, Eshrat Beigom Kia
BMC Infectious Diseases.2020;[Epub] CrossRef
Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences
Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi
Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef
Phylogenetic analysis of Trichostrongylus vitrinus isolates from southwest Iran
Mohammad Amin Ghatee, Seyed Ali Asghar Malek Hosseini, Masoud Marashifard, Mehdi Karamian, Walter Robert Taylor, Ali Jamshidi, Iraj Mobedi, Hasan Azarmehr
Parasites & Vectors.2020;[Epub] CrossRef
Clinical and haematological characteristics of human trichostrongyliasis
L. Ghanbarzadeh, M. Saraei, E.B. Kia, F. Amini, M. Sharifdini
Journal of Helminthology.2019; 93(2): 149. CrossRef
Prevalence and phylogenetic analysis of gastrointestinal helminths (Nematoda: Trichostrongylidae) in ruminant livestock of northwest Iran
Tahereh BARGHANDAN, Elham HAJİALİLO, Meysam SHARİFDİNİ, Amir JAVADİ
Ankara Üniversitesi Veteriner Fakültesi Dergisi.2019; 67(1): 65. CrossRef
Gastrointestinal helminths in farmers and their ruminant livestock from the Coastal Savannah zone of Ghana
Sylvia Afriyie Squire, Rongchang Yang, Ian Robertson, Irene Ayi, Daniel Sai Squire, Una Ryan
Parasitology Research.2018; 117(10): 3183. CrossRef
Strongyloides stercoralis hyperinfection in an unconscious diabetic patient with dermatomyositis
Meysam Sharifdini, Aniseh Hesari, SeifAli Mahdavi, Akram Alipour, EshratBeigom Kia
Indian Journal of Pathology and Microbiology.2018; 61(1): 109. CrossRef
Molecular identification and phylogenetic analysis of human Trichostrongylus species from an endemic area of Iran
Meysam Sharifdini, Sedigheh Derakhshani, Safar Ali Alizadeh, Laleh Ghanbarzadeh, Hamed Mirjalali, Iraj Mobedi, Mehrzad Saraei
Acta Tropica.2017; 176: 293. CrossRef

14,664 View
223 Download
18 Web of Science
Crossref

Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea: Seobo Sim, Jua Won, Jae-Whan Kim, Kyungjin Kim, Woo-Yoon Park, Jae-Ran Yu; Korean J Parasitol 2017;55(2):137-142.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.137

Abstract
PDF

Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragmentation and nested PCR confirmed Cryptosporidium parvum and Cyclospora cayetanensis, respectively. In conclusion, we detected C. cayetanensis in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea.

Citations

Citations to this article as recorded by

Cryptosporidium and cryptosporidiosis: An update of Asian perspectives in humans, water and food, 2015–2025
Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Mohammad Reza Mahmoudi, Huma Khan, Simuzar Mamedova, Ardra Namboodiri, Frederick R. Masangkay, Panagiotis Karanis
Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100311. CrossRef
Cyclospora in humans, animals, fresh produce and water in China: implications for host specificity of Cyclospora species and zoonotic transmission of C. cayetanensis
Kangli Feng, Yaqiong Guo, Na Li, Lihua Xiao, Yaoyu Feng
One Health Advances.2025;[Epub] CrossRef
Cryptosporidium and agriculture: A review
Eleni Golomazou, Simuzer Mamedova, Aida Vafae Eslahi, Panagiotis Karanis
Science of The Total Environment.2024; 916: 170057. CrossRef
Unveiling risks in healthy food: Vegetables and fruits are linked to the distribution chain of protozoan parasites
Aida Vafae Eslahi, Simuzer Mamedova, Reghaissia Nassiba, Panagiotis Karanis
Food Microbiology.2024; 123: 104592. CrossRef
Food and Waterborne Cryptosporidiosis from a One Health Perspective: A Comprehensive Review
Munwar Ali, Yaru Ji, Chang Xu, Qazal Hina, Usama Javed, Kun Li
Animals.2024; 14(22): 3287. CrossRef
Loop mediated isothermal amplification for detection of foodborne parasites: A journey from lab to lab-on-a-chip
Fatemeh Mahdavi Abhari, Maryam Niyyati, Hamid Assadzadeh Aghdaei, Hamed Mirjalali
Food Control.2023; 143: 109251. CrossRef
Health risks of Cryptosporidium and Giardia in the application of surface water and septic tank effluent in Chinese agriculture: Impact on cancer patients identified by quantitative microbial risk assessment
Qian Huang, Shan Huang, Weijie Kuang, Jianghui Yi, Shunxin Xiao, Feng Zhao, Guosheng Xiao
Food Microbiology.2023; 111: 104213. CrossRef
Development of a new multiplex PCR to detect fecal coccidian parasite
Manish Katiyar, Reena Gulati, Nonika Rajkumari, Rakesh Singh
Indian Journal of Gastroenterology.2023; 42(2): 241. CrossRef
Contamination of fresh produce sold on the Italian market with Cyclospora cayetanensis and Echinococcus multilocularis
Alessandra Barlaam, Tamirat T. Temesgen, Kristoffer R. Tysnes, Laura Rinaldi, Nicola Ferrari, Anna R. Sannella, Giovanni Normanno, Simone M. Cacciò, Lucy J. Robertson, Annunziata Giangaspero
Food Microbiology.2021; 98: 103792. CrossRef
Detection of Cyclospora cayetanensis on bagged pre-cut salad mixes within their shelf-life and after sell by date by the U.S. food and drug administration validated method
Sonia Almeria, Alicia Shipley
Food Microbiology.2021; 98: 103802. CrossRef
Diverse Genotypes and Species of Cryptosporidium in Wild Rodent Species from the West Coast of the USA and Implications for Raw Produce Safety and Microbial Water Quality
Xunde Li, Edward Robert Atwill
Microorganisms.2021; 9(4): 867. CrossRef
Detection of Cyclospora cayetanensis, Echinococcus multilocularis, Toxocara spp. and microsporidia in fresh produce using molecular methods: – A review
B. Bartosova, B. Koudela, I. Slana
Food and Waterborne Parasitology.2021; 23: e00124. CrossRef
Causes of acute gastroenteritis in Korean children between 2004 and 2019
Eell Ryoo
Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef
An Epidemiological and Diagnostic Study of Cyclospora Cayetanensis Parasite in Anbar Province - Iraq
S S Shahatha, S A Alkubaisy, M O Mousa
IOP Conference Series: Earth and Environmental Science.2021; 904(1): 012026. CrossRef
Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries
Angela Assurian, Helen Murphy, Laura Ewing, Hediye Nese Cinar, Alexandre da Silva, Sonia Almeria
Food Microbiology.2020; 87: 103397. CrossRef
A Molecular Tool for Rapid Detection and Traceability of Cyclospora cayetanensis in Fresh Berries and Berry Farm Soils
Carolina N. Resendiz-Nava, Guadalupe E. Orozco-Mosqueda, Edmundo M. Mercado-Silva, Susana Flores-Robles, Hilda V. Silva-Rojas, Gerardo M. Nava
Foods.2020; 9(3): 261. CrossRef
Parasite detection in food: Current status and future needs for validation
Rachel M. Chalmers, Lucy J. Robertson, Pierre Dorny, Suzanne Jordan, Age Kärssin, Frank Katzer, Stéphanie La Carbona, Marco Lalle, Brian Lassen, Ivona Mladineo, Miroslaw Rozycki, Ewa Bilska-Zajac, Gereon Schares, Anne Mayer-Scholl, Chiara Trevisan, Kristo
Trends in Food Science & Technology.2020; 99: 337. CrossRef
Detection of Cryptosporidium oocysts and Giardia cysts in vegetables from street markets from the Qinghai Tibetan Plateau Area in China
Xiuping Li, Xueyong Zhang, Yingna Jian, Geping Wang, Liqing Ma, Chad Schou, Panagiotis Karanis
Parasitology Research.2020; 119(6): 1847. CrossRef
Comparison of commercial and in-house real-time PCR platforms for 15 parasites and microsporidia in human stool samples without a gold standard
Thomas Köller, Andreas Hahn, Enkhtsetseg Altangerel, Jaco J. Verweij, Olfert Landt, Simone Kann, Denise Dekker, Jürgen May, Ulrike Loderstädt, Andreas Podbielski, Hagen Frickmann
Acta Tropica.2020; 207: 105516. CrossRef
Prevalence of Cryptosporidium and Giardia in vegetables in Iran: a nineteen-years meta-analysis review
Ehsan Javanmard, Elnaz Sadat Mirsamadi, Meysam Olfatifar, Erfan Ghasemi, Fatemeh Saki, Hamed Mirjalali, Mohammad Reza Zali, Panagiotis Karanis
Journal of Environmental Health Science and Engineering.2020; 18(2): 1629. CrossRef
Detection of human intestinal protozoan parasites in vegetables and fruits: a review
Junqiang Li, Zhenzhen Wang, Md Robiul Karim, Longxian Zhang
Parasites & Vectors.2020;[Epub] CrossRef
Human cyclosporiasis
Annunziata Giangaspero, Robin B Gasser
The Lancet Infectious Diseases.2019; 19(7): e226. CrossRef
Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay
Karen Shapiro, Minji Kim, Veronica B. Rajal, Michael J. Arrowood, Andrea Packham, Beatriz Aguilar, Stefan Wuertz
Food Microbiology.2019; 84: 103252. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
Genotyping genetically heterogeneousCyclospora cayetanensisinfections to complement epidemiological case linkage
Joel L. N. Barratt, Subin Park, Fernanda S. Nascimento, Jessica Hofstetter, Mateusz Plucinski, Shannon Casillas, Richard S. Bradbury, Michael J. Arrowood, Yvonne Qvarnstrom, Eldin Talundzic
Parasitology.2019; 146(10): 1275. CrossRef
Identification of human pathogenic Enterocytozoon bieneusi, Cyclospora cayetanensis, and Cryptosporidium parvum on the surfaces of vegetables and fruits in Henan, China
Junqiang Li, Ke Shi, Fangfang Sun, Tingwen Li, Rongjun Wang, Sumei Zhang, Fuchun Jian, Changshen Ning, Longxian Zhang
International Journal of Food Microbiology.2019; 307: 108292. CrossRef
Cyclospora cayetanensis and Cyclosporiasis: An Update
Sonia Almeria, Hediye N. Cinar, Jitender P. Dubey
Microorganisms.2019; 7(9): 317. CrossRef
Cryptosporidium spp., prevalence, molecular characterisation and socio-demographic risk factors among immigrants in Qatar
Sonia Boughattas, Jerzy M. Behnke, Duaa Al-Sadeq, Ahmed Ismail, Marawan Abu-Madi, Christine A. Petersen
PLOS Neglected Tropical Diseases.2019; 13(10): e0007750. CrossRef
Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
The Korean Journal of Parasitology.2019; 57(5): 531. CrossRef
Assessment of pesticide residues and microbial contamination in raw leafy green vegetables marketed in Italy
Gino Angelo Santarelli, Giacomo Migliorati, Francesco Pomilio, Cristina Marfoglia, Patrizia Centorame, Antonella D'Agostino, Roberta D'Aurelio, Rossana Scarpone, Noemi Battistelli, Federica Di Simone, Giuseppe Aprea, Luigi Iannetti
Food Control.2018; 85: 350. CrossRef
Parasite contamination of berries: Risk, occurrence, and approaches for mitigation
Tamirat Tefera, Kristoffer R. Tysnes, Kjersti Selstad Utaaker, Lucy J. Robertson
Food and Waterborne Parasitology.2018; 10: 23. CrossRef

11,503 View
281 Download
33 Web of Science
Crossref

Differential Protein Expressions in Virus-Infected and Uninfected Trichomonas vaginalis: Ding He, Gong Pengtao, Yang Ju Li Jianhua, Li He Zhang Guocai, Zhang Xichen; Korean J Parasitol 2017;55(2):121-128.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.121

Abstract
PDF

Protozoan viruses may influence the function and pathogenicity of the protozoa. Trichomonas vaginalis is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, T. vaginalis virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of T. vaginalis, we detected 2 strains of T. vaginalis; the virus-infected (V⁺) and uninfected (V^-) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V⁺ compared with V^- isolates. The results showed that the expression of 29 proteins was increased, and 21 proteins decreased in V⁺ isolates. These differentially expressed proteins can be classified into 4 categories: ribosomal proteins, metabolic enzymes, heat shock proteins, and putative uncharacterized proteins. Quantitative PCR was used to detect 4 metabolic processes proteins: glycogen phosphorylase, malate dehydrogenase, triosephosphate isomerase, and glucose-6-phosphate isomerase, which were differentially expressed in V⁺ and V^- isolates. Our findings suggest that mRNA levels of these genes were consistent with protein expression levels. This study was the first which analyzed protein expression variations upon TVV infection. These observations will provide a basis for future studies concerning the possible roles of these proteins in host-parasite interactions.

Citations

Citations to this article as recorded by

Role of parasite extracellular vesicles/exosomes in the interaction between hosts and virus-infected flagellate protozoa: Progress and prospects
Lu Li, Xiaocen Wang, Jianhua Li, Xichen Zhang, Xin Li, Nan Zhang, Lili Cao, Pengtao Gong
Animals and Zoonoses.2025;[Epub] CrossRef
Trichomonas vaginalis Virus: Current Insights and Emerging Perspectives
Keonte J. Graves, Jan Novak, Christina A. Muzny
Viruses.2025; 17(7): 898. CrossRef
Presence of Protozoan Viruses in Vaginal Samples from Pregnant Women and Their Association with Trichomoniasis
Gegham Ghardyan, Lusine Abrahamyan, Karen Julhakyan, Hakob Davtyan, Norayr Martirosyan, Elina Arakelova, Hranush Avagyan, Sona Hakobyan, Tigranuhi Vardanyan, Naira Karalyan, Zaven Karalyan
Pathogens.2025; 14(8): 764. CrossRef
Identification of an uncharacterized protein as a novel regulator of Giardia lamblia virus (GLV) infection in Giardia duodenalis
Zhiteng Zhao, Lili Cao, Jianqi Yuan, Shaoxiong Liu, Min Sun, Xin Li, Xiaocen Wang, Nan Zhang, Jianhua Li, Xichen Zhang, Pengtao Gong, Monique M. van Oers
Journal of Virology.2025;[Epub] CrossRef
The consequences of viral infection on protists
Victoria Fulgencio Queiroz, Juliana Miranda Tatara, Bruna Barbosa Botelho, Rodrigo Araújo Lima Rodrigues, Gabriel Magno de Freitas Almeida, Jonatas Santos Abrahao
Communications Biology.2024;[Epub] CrossRef
ՏՐԻԽՈՄՈՆԱՍ ՎԱԳԻՆԱԼԻՍ ՎԻՐՈՒՍԻ (TVV) ԱԶԴԵՑՈՒԹՅՈՒՆԸ ԿԱՆԱՆՑ ՄԻԶԱՍԵՌԱԿԱՆ ՏՐԻԽՈՄՈՆԻԱԶԻ ՎՐԱ
G.K. Ghardyan
MEDICINE, SCIENCE AND EDUCATION.2024; (37): 70. CrossRef
Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae)
Alexandra Ibañez-Escribano, Maria Teresa Gomez-Muñoz, Marta Mateo, Cristina Fonseca-Berzal, Esperanza Gomez-Lucia, Raquel Garcia Perez, Jose M. Alunda, Javier Carrion
Veterinary Sciences.2024; 11(7): 321. CrossRef
Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis
Christine Aubrey C. Justo, Miriam Jauset-Rubio, Marketa Svobodova, Vasso Skouridou, Piet Cools, Guy Mulinganya, Alexandra Ibáñez-Escribano, Windell L. Rivera, Ciara K. O'Sullivan
Analytical Biochemistry.2024; 695: 115656. CrossRef
PROTOZOONLARIN VİRAL ENDOSİMBİYONTLARI
Ayşegül DAMLAPINAR, Kader YILDIZ
Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni.2023; 14(1): 25. CrossRef
Multiple Regulations of Parasitic Protozoan Viruses: A Double-Edged Sword for Protozoa
Zhiteng Zhao, Xin Li, Nan Zhang, Jianhua Li, Na Zhao, Mengyao Gao, Xichen Zhang, Xiaocen Wang, Panpan Zhao, Lu Li, Min Sun, Lili Cao, Pengtao Gong, Vinayaka R. Prasad
mBio.2023;[Epub] CrossRef
Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus
Ravi Kumar Narayanasamy, Petr Rada, Alois Zdrha, Marc van Ranst, Johan Neyts, Jan Tachezy
Journal of Microbiology, Immunology and Infection.2022; 55(2): 191. CrossRef
Viral endosymbiotic infection of protozoan parasites: How it influences the development of cutaneous leishmaniasis
Andrea Lafleur, Martin Olivier, Neal Silverman
PLOS Pathogens.2022; 18(11): e1010910. CrossRef
Viruses of protozoan parasites and viral therapy: Is the time now right?
Paul Barrow, Jean Claude Dujardin, Nicolas Fasel, Alex D. Greenwood, Klaus Osterrieder, George Lomonossoff, Pier Luigi Fiori, Robert Atterbury, Matteo Rossi, Marco Lalle
Virology Journal.2020;[Epub] CrossRef
Trichomonas vaginalis: pathogenesis and its role in cervical cancer
José Núñez-Troconis
Investigación Clínica.2020; 61(4): 349. CrossRef
Trichomonas vaginalis virus: a review of the literature
KJ Graves, AP Ghosh, PJ Kissinger, CA Muzny
International Journal of STD & AIDS.2019; 30(5): 496. CrossRef
Trichomonas vaginalis Virus Among Women With Trichomoniasis and Associations With Demographics, Clinical Outcomes, and Metronidazole Resistance
Keonte J Graves, Arindam P Ghosh, Norine Schmidt, Peter Augostini, W Evan Secor, Jane R Schwebke, David H Martin, Patricia J Kissinger, Christina A Muzny
Clinical Infectious Diseases.2019; 69(12): 2170. CrossRef

9,655 View
180 Download
14 Web of Science
Crossref

Brief Communications

Seroprevalence of Toxoplasma gondii among HIV Patients in Jahrom, Southern Iran: Hassan Rezanezhad, Fateme Sayadi, Enayatollah Shadmand, Seyed Dawood Mousavi Nasab, Hadi Rezaei Yazdi, Kavous Solhjoo, Akbar Kazemi, Monireh Maleki, Abbas Ahmadi Vasmehjani; Korean J Parasitol 2017;55(1):99-103.
Published online February 28, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.1.99

Abstract
PDF

Toxoplasma gondii is an important opportunistic agent especially in immunocompromised hosts and can cause significant morbidity and mortality. Hence, detection and monitoring of anti-Toxoplasma antibodies are of a great interest in HIV-infected patients. A study on the prevalence of toxoplasmosis and associated risk factors was carried out among HIV-infected patients in Jahrom, southern Iran. The prevalence of anti-Toxoplasma IgG antibodies was 21.1% in HIV-infected patients by ELISA. PCR was performed on all of the samples, and 1 of the blood samples was positively detected. Among the HIV patients, anti-Toxoplasma IgG antibodies were significantly higher in age group of 30-39 years old (P=0.05). The seroprevalence of toxoplasmosis in patients with CD4+<100 cells/μl was 33.3% that was significantly higher than the other groups (P=0.042) with or without IgG antibodies. The CD4+ count mean of seropositive patients was lower than that of seronegative patients. The seroprevalence of toxoplasmosis in patients with highly active antiretroviral therapy was significantly less than patients without therapy (P=0.02). In conclusion, this study showed low seroprevalence of latent toxoplasmosis among HIV-infected patients in the region and confirmed the need for intensifying prevention efforts among this high-risk population and also the risk of toxoplasmosis reactivation which could be important among this population.

Citations

Citations to this article as recorded by

The Global Prevalence of and Factors Associated with Parasitic Coinfection in People Living with Viruses: A Systematic Review and Meta-Analysis
Yan Ge, Huaman Liu, Ningjun Ren, Abdul Qadeer, Ian Kim B. Tabios, Ian Kendrich C. Fontanilla, Lydia R. Leonardo, Banchob Sripa, Guofeng Cheng
Pathogens.2025; 14(6): 534. CrossRef
Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
Maryam Nejabat, Mohammadreza Heydari, Mohammad Motamedifar, Zohre Foroozanfar, Saeid Amirizadeh Fard, Ava Hashempour, Nazani Nazari, Esmaeil Rezaei, Zahra Heydari
Immunity, Inflammation and Disease.2024;[Epub] CrossRef
RPA-CRISPR/Cas9-based method for the detection of Toxoplasma gondii: A proof of concept
Mengchen Wu, Haiyan Wu, Xueqiu Chen, Fei Wu, Guangxu Ma, Aifang Du, Yi Yang
Veterinary Parasitology.2024; 327: 110115. CrossRef
Toxoplasma gondii, HBV, and HCV co‐infection and their correlation with CD4 cells among Iranian HIV‐positive patients
Ahmadreza Bazmjoo, Mohammad Aref Bagherzadeh, Rahim Raoofi, Ali Taghipour, Samaneh Mazaherifar, Hojatallah Sotoodeh, Zahra Ostadi, Enayat Shadmand, Mirza Ali Mofazzal Jahromi, Amir Abdoli
Immunity, Inflammation and Disease.2023;[Epub] CrossRef
Prevalence of Toxoplasma gondii in HIV-Infected Individuals in Iran: A Systematic Review and Meta-Analysis
Pegah Shakib, Masoud Behzadifar, Hossein Mahmoudvand, Sara Larki, Hamed Kalani, Masoomeh Zivdari, Omid Mardanshah, Kourosh Cheraghipour
Current HIV Research.2023; 21(4): 221. CrossRef
Seroprevalence of Toxoplasma gondii in people at an increased risk of HIV infection – a pilot study
Małgorzata Smoguła, Marta Pawłowska, Roland Wesołowski, Karolina Szewczyk-Golec, Celestyna Mila-Kierzenkowska
Diagnostyka Laboratoryjna.2022; 58(1): 9. CrossRef
Serological Evaluation of Toxoplasmosis and Related Risk Factors Among HIV+/AIDS Patients in Northern Iran
Hasan Rafati-Sajedi, Bijan Majidi-Shad, Reza Jafari-Shakib, Zahra Atrkar-Roshan, Mohammad Reza Mahmoudi, Seyed Mahmood Rezvani
Acta Parasitologica.2021; 66(4): 1417. CrossRef
Investigating the risk factors for seroprevalence and the correlation between CD4+ T-cell count and humoral antibody responses to Toxoplasma gondii infection amongst HIV patients in the Bamenda Health District, Cameroon
Eugene Enah Fang, Raymond Babila Nyasa, Emmanuel Menang Ndi, Denis Zofou, Tebit Emmanuel Kwenti, Edith Pafoule Lepezeu, Vincent P. K. Titanji, Roland N. Ndip, Mohammed Abdelfatah Mosa Alhoot
PLOS ONE.2021; 16(12): e0256947. CrossRef
The first report of the evaluation of the knowledge regarding toxoplasmosis among health professionals in public health centers in Rabat, Morocco
Majda Laboudi, Sanaa Ait Hamou, Imane Mansour, Ilham Hilmi, Abderrahim Sadak
Tropical Medicine and Health.2020;[Epub] CrossRef
Toxoplasmosis Among HIV Patients and Healthy Volunteers in Port Harcourt, Rivers State, Nigeria
Evelyn O Onosakponome, Austin E Abah, Michael Wogu
International Journal of Infection.2020;[Epub] CrossRef
Seroprevalence of Toxoplasma gondii in HIV-infected patients admitted to a university hospital
Pinar ERGEN, Fatma YILMAZ-KARADAĞ, Özlem AYDIN
Journal of Health Sciences and Medicine.2020; 3(4): 372. CrossRef
Sero-molecular evaluation of Toxoplasma gondii infection among HIV-positive patients
Ehsan Ahmadpour, Reza Pishkarie-Asl, Adel Spotin, Hossein Samadi Kafil, Hasan Didarlu, Yaghob Azadi, Aleksandra Barac
Transactions of The Royal Society of Tropical Medicine and Hygiene.2019; 113(12): 771. CrossRef
Toxoplasma gondii: The Prevalence and Risk Factors in HIV-Infected Patients in Fars Province, Southern Iran
Nasir Arefkha, Bahador Sarkari, Masood Afrashteh, Zahra Rezaei, Mona Dehghani
Iranian Red Crescent Medical Journal.2018;[Epub] CrossRef

9,901 View
205 Download
16 Web of Science
Crossref

Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea: Jae-Hyung Lee, Juan-Hua Quan, In-Wook Choi, Gab-Man Park, Guang-Ho Cha, Hyun-Ju Kim, Jae-Min Yuk, Young-Ha Lee; Korean J Parasitol 2017;55(1):95-98.
Published online February 28, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.1.95

Abstract
PDF

Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 402 samples, F. hepatica 1TS-2 marker was detected in 6 freshwater snails; thus, the overall prevalence in freshwater snails was 1.5%. The prevalence varied between collection areas, ranging from 0.0% at Hoenggye-ri to 2.9% at Suha-ri. However, F. gigantica ITS-2 was not detected in the 6 F. hepatica-positive samples by PCR. The nucleotide sequences of the 6 F. hepatica ITS-2 PCR-positive samples were 99.4% identical to the F. hepatica ITS-2 sequences in GenBank, whereas they were 98.4% similar to F. gigantica ITS-2 sequences. These results indicated that the prevalence of F. hepatica in snail intermediate hosts was 1.5% in Gangwon-do, Korea; however the prevalence varied between collection areas. These results may help us to understand F. hepatica infection status in natural environments.

Citations

Citations to this article as recorded by

Fasciola hepatica infection in Korean water deer (Hydropotes inermis argyropus)
Na-Hyeon Kim, Min-Gyeong Seo, Bumseok Kim, Yu Jeong Jeon, In Jung Jung, Il-Hwa Hong
Parasites, Hosts and Diseases.2025; 63(3): 243. CrossRef
Countering Zoonotic Diseases: Current Scenario and Advances in Diagnostics, Monitoring, Prophylaxis and Therapeutic Strategies
Saurabh Gupta, Rasanpreet Kaur, Jagdip Singh Sohal, Shoor Vir Singh, Kaushik Das, Manish Kumar Sharma, Jitendra Singh, Shalini Sharma, Kuldeep Dhama
Archives of Medical Research.2024; 55(6): 103037. CrossRef
The potential of snails as a source of food and feed
Akash Gupta, Prabhat Khanal
Journal of Agriculture and Food Research.2024; 18: 101330. CrossRef
Molluscicidal and cercaricidal effects of Persicaria senegalensis on Radix natalensis snails and their echinostome-shed cercariae in South Africa
MC Mathole, PH King
African Journal of Aquatic Science.2023; 48(1): 71. CrossRef
Epidemiology of Fasciola spp. in the intermediate host in China: A potential risk for fasciolosis transmission
Ming Pan, Shao-Yuan Bai, Tian-Kai Ji, Yi-Min Fan, Dan-Dan Liu, Yi Yang, Jian-Ping Tao, Si-Yang Huang
Acta Tropica.2022; 230: 106394. CrossRef
Economic losses, morpho-molecular identification, and identity of Fasciola species recovered from Egypt
Omima Ramadan Abdel‑Fatah, Waleed M. Arafa, Ahmed Anwar Wahba, Khaled Mohamed El‑Dakhly
Journal of Parasitic Diseases.2022; 46(4): 1036. CrossRef
Green vegetable juice as a potential source of human fascioliasis in Korea
Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
One Health.2022; 15: 100441. CrossRef
Human and Animal Fascioliasis: Origins and Worldwide Evolving Scenario
Santiago Mas-Coma, M. Adela Valero, M. Dolores Bargues
Clinical Microbiology Reviews.2022;[Epub] CrossRef
Living Liver Donor With Fasciola Hepatica: First Case Report in the Literature
Sami Akbulut, Tevfik Tolga Sahin, Mehmet Kolu, Burak Isik, Yasar Bayindir, Sezai Yilmaz
Experimental and Clinical Transplantation.2021; 19(3): 276. CrossRef
Trematode cercarial fauna obtained from the field-collected freshwater snails Lymnaea natalensis in Egypt
Amina M. Ibrahim, Amira Kamal Ahmed
Bulletin of the National Research Centre.2019;[Epub] CrossRef
Assessment of the molluscicidal impact of extracted chlorophyllin on some biochemical parameters in the nervous tissue and histological changes in Biomphalaria alexandrina and Lymnaea natalensis snails
Amina M. Ibrahim, Fayez A. Bakry
Invertebrate Neuroscience.2019;[Epub] CrossRef
A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea
Hyo-Seok Kim, Joo-Yeon Kong, Jong-Hyun Kim, Seong-Chan Yeon, Il-Hwa Hong
The Korean Journal of Parasitology.2018; 56(4): 375. CrossRef
Purification and biochemical characterization of a 22-kDa stable cysteine- like protease from the excretory-secretory product of the liver fluke Fasciola hepatica by using conventional techniques
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
Journal of Chromatography B.2017; 1068-1069: 268. CrossRef

9,985 View
220 Download
12 Web of Science
Crossref

Case Report

Cutaneous Leishmaniasis of the Eyelids: A Case Series with Molecular Identification and Literature Review: Iraj Mohammadpour, Mohammad Hossein Motazedian, Farhad Handjani, Gholam Reza Hatam; Korean J Parasitol 2016;54(6):787-792.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.787

Abstract
PDF

Cutaneous leishmaniasis (CL) is a protozoan disease which is endemic in Iran. It is transmitted by the Phlebotomus sand fly. The eyelid is rarely involved possibly because the movement of the lids impedes the sand fly from biting the skin in this region. Here, we report 6 rare cases of eyelid CL. The patients were diagnosed by skin scraping, culture, and PCR from the lesions. Skin scraping examination showed Leishmania spp. amastigotes in the cytoplasm of macrophages. Culture examination was positive for Leishmania spp. PCR was positive for Leishmania major and Leishmania tropica. The lesions were disguised as basal cell carcinoma, chalazion, hordeolum, and impetigo. The patients were treated with intramuscular meglumine antimoniate (20 mg/kg/day) for at least 3 weeks. They showed a dramatic response, and the lesions almost completely disappeared. We emphasized the importance of clinical and diagnostic features of lesions, characterized the phylogenetic relationship of isolated parasites, and reviewed the literature on ocular leishmaniasis.

Citations

Citations to this article as recorded by

Management strategies for chalazia in pediatric patients: A scoping review
Tom Kornhauser, Abdelrahman M. Elhusseiny, John D. Pemberton
European Journal of Ophthalmology.2025; 35(4): 1481. CrossRef
Self-administration of herbal treatment for periocular cutaneous leishmaniasis: A case report and systematic review
Rawan N. AlThaqib, Faisal A. Altahan, Hamad M. Alsulaiman, Diego Strianese, Azza Maktabi
Saudi Journal of Ophthalmology.2025; 39(2): 174. CrossRef
Atypical Manifestations of Old World Cutaneous Leishmaniasis: A Systematic Review and Clinical Atlas of Unusual Clinical and Specific Anatomical Presentations
Bahareh Abtahi‐Naeini, Seyed Naser Emadi, Zabihollah Shahmoradi, Mahsa Pourmahdi‐Boroujeni, Ali Saffaei, Fereshte Rastegarnasab
Health Science Reports.2025;[Epub] CrossRef
Unilateral erythematous nodule over eyelid
Farzana Ansari, Akriti Agrawal, Anupama Bains, Poonam Elhence
Indian Journal of Dermatology, Venereology and Leprology.2023; 89: 928. CrossRef
Ocular Leishmaniasis - A systematic review
Guillaume Mignot, Yagnaseni Bhattacharya, Aravind Reddy
Indian Journal of Ophthalmology.2021; 69(5): 1052. CrossRef
The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis
Homa Hajjaran, Reza Saberi, Alireza Borjian, Mahdi Fakhar, Seyed Abdollah Hosseini, Sajjad Ghodrati, Mehdi Mohebali
Frontiers in Public Health.2021;[Epub] CrossRef
Dermatoses infectieuses des paupières
A. Hamerstehl, M. Verstappen, P. del Giudice
Annales de Dermatologie et de Vénéréologie - FMC.2021; 1(8): 542. CrossRef
Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis
Denis Sereno, Mohammad Akhoundi, Kourosh Sayehmri, Asad Mirzaei, Philippe Holzmuller, Veerle Lejon, Etienne Waleckx
International Journal of Molecular Sciences.2020; 21(5): 1684. CrossRef
Leishmania cytochrome b gene sequence polymorphisms in southern Iran: relationships with different cutaneous clinical manifestations
Iraj Mohammadpour, Gholam Reza Hatam, Farhad Handjani, Farzaneh Bozorg-Ghalati, Daniel PourKamal, Mohammad Hossein Motazedian
BMC Infectious Diseases.2019;[Epub] CrossRef
Case Report: Mucocutaneous Leishmaniasis Masquerading as Idiopathic Midline Granulomatous Disease
Nilesh Tejura, Eunjung Kim, Lisa L. Dever, Debra Chew
The American Journal of Tropical Medicine and Hygiene.2019; 101(5): 1107. CrossRef
Efficacy of cryotherapy plus topical Juniperus excelsa M. Bieb cream versus cryotherapy plus placebo in the treatment of Old World cutaneous leishmaniasis: A triple-blind randomized controlled clinical trial
Mohammad Mahdi Parvizi, Farhad Handjani, Mahmoodreza Moein, Gholamreza Hatam, Majid Nimrouzi, Jafar Hassanzadeh, Nasrin Hamidizadeh, Hamid Reza Khorrami, Mohammad Mehdi Zarshenas, Waleed Saleh Al-Salem
PLOS Neglected Tropical Diseases.2017; 11(10): e0005957. CrossRef

9,913 View
207 Download
11 Web of Science
Crossref

Original Article

Production of IL-1β and Inflammasome with Up-Regulated Expressions of NOD-Like Receptor Related Genes in Toxoplasma gondii-Infected THP-1 Macrophages: Jia-Qi Chu, Ge Shi, Yi-Ming Fan, In-Wook Choi, Guang-Ho Cha, Yu Zhou, Young-Ha Lee, Juan-Hua Quan; Korean J Parasitol 2016;54(6):711-717.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.711

Abstract
PDF

Toxoplasma gondii is an obligate intracellular parasite that stimulates production of high levels of proinflammatory cytokines, which are important for innate immunity. NLRs, i.e., nucleotide-binding oligomerization domain (NOD)-like receptors, play a crucial role as innate immune sensors and form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1β. To elucidate the role of inflammasome components in T. gondii-infected THP-1 macrophages, we examined inflammasome-related gene expression and mechanisms of inflammasome-regulated cytokine IL-1β secretion. The results revealed a significant upregulation of IL-1β after T. gondii infection. T. gondii infection also upregulated the expression of inflammasome sensors, including NLRP1, NLRP3, NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and NAIP, in a time-dependent manner. The infection also upregulated inflammasome adaptor protein ASC and caspase-1 mRNA levels. From this study, we newly found that T. gondii infection regulates NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and neuronal apoptosis inhibitor protein (NAIP) gene expressions in THP-1 macrophages and that the role of the inflammasome-related genes may be critical for mediating the innate immune responses to T. gondii infection.

Citations

Citations to this article as recorded by

Investigating the possible role of toxoplasmosis and Interleukin-1β variants on the immune response in Egyptian diabetic patients
Asmaa Ibrahim, Nancy O. kamel, Fatma Rageh, Rasha Elgamal, Mohamed A. Sakr, Eman M. Osman, Samar s. Ahmed, Hend A. Yassin, Yasmine N. Kamel, Reham F. Othman, Manar Ezzelarab Ramadan
Microbes and Infection.2025; 27(7): 105559. CrossRef
Early responses of primary human and bovine monocytes, monocytic THP-1 cells and THP-1 cell-derived macrophages to vital Toxoplasma gondii tachyzoites
Dominik Hanke, Zahady D. Velásquez, Kathrin Büttner, Andreas Krueger, Ralf Ross, Andreas Hecker, Sybille Mazurek, Veronika Grau, Anja Taubert, Carlos Hermosilla, Katrin Richter, Iván Conejeros
Frontiers in Immunology.2025;[Epub] CrossRef
Unraveling the NLRP family: Structure, function, activation, critical influence on tumor progression, and potential as targets for cancer therapy
Xueqing Zhou, Yongguang Tao, Ying Shi
Cancer Letters.2024; 605: 217283. CrossRef
Role of inflammasomes in Toxoplasma and Plasmodium infections
Zhi-xin Wang, Wan-jun Jiao, Yong Yang, Hong-li Liu, Hai-long Wang
Parasites & Vectors.2024;[Epub] CrossRef
NLRP13 inflammasome complex is hypermethylated in familial Mediterranean fever and global methylation correlates with the disease severity
Feyzanur Yildirimtepe Caldiran, Koksal Deveci, Ercan Cacan
Annals of Human Genetics.2023; 87(3): 115. CrossRef
Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells
Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh
Pathogens.2023; 12(2): 253. CrossRef
P2X7 Receptor Modulation of the Gut Microbiota and the Inflammasome Determines the Severity of Toxoplasma gondii-Induced Ileitis
Aline Cristina Abreu Moreira-Souza, Hayandra Ferreira Nanini, Thuany Prado Rangel, Sthefani Rodrigues Batista da Silva, Beatriz Pêgo Damasceno, Beatriz Elias Ribeiro, Cynthia M. Cascabulho, Fabiano Thompson, Camille Leal, Patrícia Teixeira Santana, Siane
Biomedicines.2023; 11(2): 555. CrossRef
The role of NOD-like receptors in innate immunity
Cássio Luiz Coutinho Almeida-da-Silva, Luiz Eduardo Baggio Savio, Robson Coutinho-Silva, David M. Ojcius
Frontiers in Immunology.2023;[Epub] CrossRef
NLRP3 Plays a Key Role in Antihelminth Immunity in the Enteral and Parenteral Stages of Trichinella spiralis-Infected Mice
Tian-Xu Pan, Hai-Bin Huang, Hui-Nan Lu, Guang-Xun Zhao, Yu Quan, Jun-Yi Li, Ying Xue, Zhi-Yu Zhu, Yue Wang, Chun-Wei Shi, Nan Wang, Gui-Lian Yang, Chun-Feng Wang, De’Broski R. Herbert
Infection and Immunity.2023;[Epub] CrossRef
Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells
Hossein Pazoki, Hamed Mirjalali, Maryam Niyyati, Seyed Javad Seyed Tabaei, Nariman Mosaffa, Shabnam Shahrokh, Hamid Asadzadeh Ahdaei, Andreas Kupz, Mohammad Reza Zali
Microbial Pathogenesis.2023; 180: 106120. CrossRef
Possible therapeutic targets for NLRP3 inflammasome-induced breast cancer
Xixi Wang, Junyi Lin, Zhe Wang, Zhi Li, Minghua Wang
Discover Oncology.2023;[Epub] CrossRef
Toxoplasma gondii Induces Pyroptosis in Human Placental Trophoblast and Amniotic Cells by Inducing ROS Production and Activation of Cathepsin B and NLRP1/NLRP3/NLRC4/AIM2 Inflammasome
Juan-Hua Quan, Fei Fei Gao, Tian-Zhong Ma, Wei Ye, Xiang Gao, Ming-Zhu Deng, Lan-Lan Yin, In-Wook Choi, Jae-Min Yuk, Guang-Ho Cha, Young-Ha Lee, Jia-Qi Chu
The American Journal of Pathology.2023; 193(12): 2047. CrossRef
Role of Microgliosis and NLRP3 Inflammasome in Parkinson’s Disease Pathogenesis and Therapy
Fillipe M. de Araújo, Lorena Cuenca-Bermejo, Emiliano Fernández-Villalba, Silvia L. Costa, Victor Diogenes A. Silva, Maria Trinidad Herrero
Cellular and Molecular Neurobiology.2022; 42(5): 1283. CrossRef
Latent Upregulation of Nlrp3, Nlrc4 and Aim2 Differentiates between Asymptomatic and Symptomatic Trichomonas vaginalis Infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Immunological Investigations.2022; 51(5): 1127. CrossRef
P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro
Qi Tan, Qing Ai, Yu He, Fang Li, Jialin Yu
Microbial Pathogenesis.2022; 164: 105379. CrossRef
In silico analysis of expression and DNA methylation profiles of NLRP13 inflammasome in tumor cells
Feyzanur Yildirimtepe Caldiran, Caglar Berkel, Koksal Deveci, Ercan Cacan
Human Gene.2022; 33: 201067. CrossRef
The strategies of NLRP3 inflammasome to combat Toxoplasma gondii
Chanjin Yoon, Yu Seong Ham, Woo Jin Gil, Chul-Su Yang
Frontiers in Immunology.2022;[Epub] CrossRef
Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii-infected human small intestinal epithelial cells
Jia-Qi Chu, Fei Fei Gao, Weiyun Wu, Chunchao Li, Zhaobin Pan, Jinhui Sun, Hao Wang, Cong Huang, Sang Hyuk Lee, Juan-Hua Quan, Young-Ha Lee
Parasites & Vectors.2021;[Epub] CrossRef
RIPK3 Facilitates Host Resistance to Oral Toxoplasma gondii Infection
Patrick W. Cervantes, Bruno Martorelli Di Genova, Billy Joel Erazo Flores, Laura J. Knoll, Jeroen P. J. Saeij
Infection and Immunity.2021;[Epub] CrossRef
Soluble total antigen derived from Toxoplasma gondii tachyzoites increased the expression levels of NLRP1, NLRP3, NLRC4, AIM2, and the release of mature form of IL1β, but downregulated the expression of IL1β and IL18 genes in THP-1cell line
Hossein Pazoki, Hanieh Mohammad Rahimi, Hamed Mirjalali, Maryam Niyyati, Nariman Mosaffa, Seyed Javad Seyed Tabaei, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Microbial Pathogenesis.2021; 158: 105072. CrossRef
Host inflammatory responses to intracellular invaders: Review study
Ramesh Chandra Rai
Life Sciences.2020; 240: 117084. CrossRef
Treatment of mice with S4B6 IL-2 complex prevents lethal toxoplasmosis via IL-12- and IL-18-dependent interferon-gamma production by non-CD4 immune cells
Andreas Kupz, Saparna Pai, Paul R. Giacomin, Jennifer A. Whan, Robert A. Walker, Pierre-Mehdi Hammoudi, Nicholas C. Smith, Catherine M. Miller
Scientific Reports.2020;[Epub] CrossRef
Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages
Marta García-Sánchez, Laura Jiménez-Pelayo, Pilar Horcajo, Esther Collantes-Fernández, Luis Miguel Ortega-Mora, Javier Regidor-Cerrillo
Parasites & Vectors.2020;[Epub] CrossRef
A Comparison of Transcriptional Diversity of Swine Macrophages Infected With TgHB1 Strain of Toxoplasma gondii Isolated in China
Yongle Song, Lindong Song, Xiaoting Wan, Bang Shen, Rui Fang, Min Hu, Junlong Zhao, Yanqin Zhou
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Cytosolic Recognition of Microbes and Pathogens: Inflammasomes in Action
Jenni A. Hayward, Anukriti Mathur, Chinh Ngo, Si Ming Man
Microbiology and Molecular Biology Reviews.2018;[Epub] CrossRef
P2X7 receptor mediates NLRP3-dependent IL-1β secretion and parasite proliferation in Toxoplasma gondii-infected human small intestinal epithelial cells
Juan-Hua Quan, Rui Huang, Zhuang Wang, Shuai Huang, In-Wook Choi, Yu Zhou, Young-Ha Lee, Jia-Qi Chu
Parasites & Vectors.2018;[Epub] CrossRef

12,694 View
294 Download
27 Web of Science
Crossref

Brief Communication

Epidemiological Characteristics of Strongyloidiasis in Inhabitants of Indigenous Communities in Borneo Island, Malaysia: Romano Ngui, Noor Amira Abdul Halim, Yamuna Rajoo, Yvonne AL Lim, Stephen Ambu, Komalaveni Rajoo, Tey Siew Chang, Lu Chan Woon, Rohela Mahmud; Korean J Parasitol 2016;54(5):673-378.
Published online October 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.5.673

Abstract
PDF

Epidemiological study on strongyloidiasis in humans is currently lacking in Malaysia. Thus, a cross-sectional study was carried out to determine the prevalence of Strongyloides stercoralis infection among the inhabitants of longhouse indigenous communities in Sarawak. A single stool and blood sample were collected from each participant and subjected to microscopy, serological and molecular techniques. Five species of intestinal parasites were identified by stool microscopy. None of the stool samples were positive for S. stercoralis. However, 11% of 236 serum samples were seropositive for strongyloidiasis. Further confirmation using molecular technique on stool samples of the seropositive individuals successfully amplified 5 samples, suggesting current active infections. The prevalence was significantly higher in adult males and tended to increase with age. S. stercoralis should no longer be neglected in any intestinal parasitic survey. Combination of more than 1 diagnostic technique is necessary to increase the likelihood of estimating the ‘true’ prevalence of S. stercoralis.

Citations

Citations to this article as recorded by

Strongyloides seroprevalence in patients with advanced chronic kidney disease at a tertiary hospital in Malaysia: Epidemiological profiles and comparison of two assays
Emelia Osman, Rahmah Noordin, Rozita Mohd, Lydia Kamaruzaman, Siti Nor Azreen Abdul Manap, S. Izuddin Mohd. Zali, Nur Afrina Azhar, Nor Suhada Anuar
Parasite Epidemiology and Control.2025; 31: e00458. CrossRef
Seroprevalence and associated risk factors of strongyloidiasis in indigenous communities and healthcare professionals from Brazil
Vamilton Alvares Santarém, Fernando Rodrigo Doline, João Henrique Farinhas dos Santos, Isabella Braghin Ferreira, Bruna Barroso Gomes, Dirce Mary Correa Meisel, Leandro Meneguelli Biondo, Susana Angélica Zevallos Lescano, Ronaldo Cesar Borges Gryschek, Ro
PLOS Neglected Tropical Diseases.2023; 17(4): e0011283. CrossRef
Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis
Cíntia de Lima Oliveira, Joelma Nascimento de Souza, Alex Bruno da Silva Souza, Nilo Manoel Pereira Vieira Barreto, Irlana Dias Ribeiro, Larissa Mota Sampaio, Weslei Almeida Costa Araújo, Juliane Silva Batista dos Santos, Márcia Cristina Aquino Teixeira,
Acta Parasitologica.2022; 67(2): 732. CrossRef
Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays
YuYen L. Chan, Catriona L. Patterson, Jeffrey W. Priest, Gillian Stresman, Timothy William, Tock H. Chua, Kevin Tetteh, Patrick Lammie, Chris Drakeley, Kimberly M. Fornace
Frontiers in Public Health.2022;[Epub] CrossRef
Seropositivity and geographical distribution of Strongyloides stercoralis in Australia: A study of pathology laboratory data from 2012–2016
Jennifer Shield, Sabine Braat, Matthew Watts, Gemma Robertson, Miles Beaman, James McLeod, Robert W. Baird, Julie Hart, Jennifer Robson, Rogan Lee, Stuart McKessar, Suellen Nicholson, Johanna Mayer-Coverdale, Beverley-Ann Biggs, Subash Babu
PLOS Neglected Tropical Diseases.2021; 15(3): e0009160. CrossRef
Prevalence and risk factors of strongyloidiasis among schoolchildren in Sabach Sanjal and Upper Badibou districts in the North Bank East Region of The Gambia
Abdoulie M. Sanyang, Ebrima Joof, Alhagie Papa Sey, Sana Sambou, Zeehaida Mohamed, Bakary Sanneh
Parasite Epidemiology and Control.2021; 15: e00228. CrossRef
Serological assays for the diagnosis of Strongyloides stercoralis infection: a systematic review and meta-analysis of diagnostic test accuracy
Narges Kalantari, Mohammad Chehrazi, Salman Ghaffari, Tahmineh Gorgani-Firouzjaee
Transactions of The Royal Society of Tropical Medicine and Hygiene.2020; 114(6): 459. CrossRef
Prevalence and risk factors of Strongyloides stercoralis infection among Orang Asli schoolchildren: new insights into the epidemiology, transmission and diagnosis of strongyloidiasis in Malaysia
Hesham M. Al-Mekhlafi, Nabil A. Nasr, Yvonne A. L. Lim, Fatin Nur Elyana, Hany Sady, Wahib M. Atroosh, Salwa Dawaki, Tengku Shahrul Anuar, Rahmah Noordin, Rohela Mahmud
Parasitology.2019; 146(12): 1602. CrossRef
A possible need for routine screening for Strongyloides stercoralis infection in Indian haemophilia patients
Rucha K. Patil, Kanjaksha K. Ghosh, S. Chandrakala, Shrimati Shetty
Indian Journal of Medical Research.2018; 147(3): 315. CrossRef
Soil-Transmitted Helminths in Tropical Australia and Asia
Catherine Gordon, Johanna Kurscheid, Malcolm Jones, Darren Gray, Donald McManus
Tropical Medicine and Infectious Disease.2017; 2(4): 56. CrossRef

9,601 View
148 Download
14 Web of Science
Crossref

Original Articles

Differential Expression of Hox and Notch Genes in Larval and Adult Stages of Echinococcus granulosus: Ebrahim Saedi Dezaki, Mohammad Mehdi Yaghoobi, Elham Taheri, Pooya Ghaseminejad Almani, Farideh Tohidi, Bruno Gottstein, Majid Fasihi Harandi; Korean J Parasitol 2016;54(5):653-658.
Published online October 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.5.653

Abstract
PDF

This investigation aimed to evaluate the differential expression of HoxB7 and notch genes in different developmental stages of Echinococcus granulosus sensu stricto. The expression of HoxB7 gene was observed at all developmental stages. Nevertheless, significant fold differences in the expression level was documented in the juvenile worm with 3 or more proglottids, the germinal layer from infected sheep, and the adult worm from an experimentally infected dog. The notch gene was expressed at all developmental stages of E. granulosus; however, the fold difference was significantly increased at the microcysts in monophasic culture medium and the germinal layer of infected sheep in comparison with other stages. The findings demonstrated that the 2 aforementioned genes evaluated in the present study were differentially expressed at different developmental stages of the parasite and may contribute to some important biological processes of E. granulosus.

Citations

Citations to this article as recorded by

Evolution and Function of the Notch Signaling Pathway: An Invertebrate Perspective
Yan Lv, Xuan Pang, Zhonghong Cao, Changping Song, Baohua Liu, Weiwei Wu, Qiuxiang Pang
International Journal of Molecular Sciences.2024; 25(6): 3322. CrossRef
Echinococcus multilocularis delta/notch signalling components are expressed in post-mitotic cells
Chris Speicher, Monika Bergmann, Klaus Brehm
Parasitology Research.2024;[Epub] CrossRef
Notch signaling pathway involved in Echinococcus granulosus infection regulates dendritic cell development and differentiation
Mingxia Wang, Zailing Shang, Fei Qiao, Junhu Hei, Xueling Ma, Yana Wang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis
Guilherme Brzoskowski dos Santos, Edileuza Danieli da Silva, Eduardo Shigueo Kitano, Maria Eduarda Battistella, Karina Mariante Monteiro, Jeferson Camargo de Lima, Henrique Bunselmeyer Ferreira, Solange Maria de Toledo Serrano, Arnaldo Zaha
Parasites & Vectors.2022;[Epub] CrossRef
The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis
Raúl J. Bobes, Karel Estrada, Diana G. Rios-Valencia, Arturo Calderón-Gallegos, Patricia de la Torre, Julio C. Carrero, Alejandro Sanchez-Flores, Juan P. Laclette
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Bioinformatics analysis and experimental verification of Notch signalling pathway-related miRNA–mRNA subnetwork in extracellular vesicles during Echinococcus granulosus encystation
Jian Gao, Xuan Zhou, Ling Liu, Guodong Lv, Qiulian Hou, Xiaofan Zhang, Yujuan Shen
Parasites & Vectors.2022;[Epub] CrossRef
Screening, construction, and serological identification of the diagnostic antigen molecule EG-06283 for the diagnosis of Echinococcus granulosus
Yazhou Zhu, Tingrui Zhang, Yinqi Zhao, Yin Wang, Yongxue Lv, Shasha Li, Songhao Yang, Mingxing Zhu, Wei Zhao, Qiang Wang
Parasitology Research.2021; 120(7): 2557. CrossRef
Evolutionary Transformations of the Metazoan Body Plan: Genomic-Morphogenetic Correlations
V. V. Isaeva, S. V. Rozhnov
Paleontological Journal.2021; 55(7): 811. CrossRef
In vitro effects of glutathione on Transforming Growth Factor beta and Epidermal Growth Factor Receptor genes expression in the protoscoleces and strobilated worms of Echinococcus granulosus
Seyyed Jafar Nosratabadi, Nasim Hayati Roodbari, Ali Afgar, Mohammad Hossein Modarressi, Alireza Farsinejad, Majid Fasihi Harandi
Gene Reports.2020; 20: 100786. CrossRef
Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti
T. Basika, G.P. Paludo, F.M. Araujo, A.C. Salim, F. Pais, L. Maldonado, N. Macchiaroli, J. Camargo de Lima, M. Rosenzvit, G.C. Oliveira, L. Kamenetzky, H.B. Ferreira
Molecular and Biochemical Parasitology.2019; 229: 35. CrossRef
Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus
Seyed Mohammad Mousavi, Ali Afgar, Mohammad Ali Mohammadi, Seifollah Mortezaei, Balal Sadeghi, Majid Fasihi Harandi
Scientific Reports.2019;[Epub] CrossRef
The effect of albendazole sulfoxide on the expression of miR-61 and let-7 in different in vitro developmental stages of Echinococcus granulosus
Seifollah Mortezaei, Ali Afgar, Mohammad Ali Mohammadi, Seyed Mohammad Mousavi, Balal Sadeghi, Majid Fasihi Harandi
Acta Tropica.2019; 195: 97. CrossRef
Comparative Transcriptomic Analysis of the Larval and Adult Stages of Taenia pisiformis
Shaohua Zhang
Genes.2019; 10(7): 507. CrossRef

9,513 View
191 Download
15 Web of Science
Crossref

Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea: Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin; Korean J Parasitol 2016;54(5):631-636.
Published online October 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.5.631

Abstract
PDF

This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively. The multiplex-touchdown PCR protocol using a primer mixture simultaneously detected protozoa in human stools, and the amplified gene was detected in >1×10³ oocysts for C. parvum, >1×10⁴ cysts for G. lamblia, and >1 copy of the 18S rRNA gene for C. cayetanensis. Taken together, our protocol convincingly demonstrated the ability to simultaneously detect C. parvum, G. lamblia, and C. cayetanenesis in stool samples.

Citations

Citations to this article as recorded by

A Multiplex RT-PCR for the Detection of Three Viruses and One Viroid Infecting Hemp
Derrick J. Grunwald, Jacob MacWilliams, Laine Hackenberg, Sydney Stroschein, Renee Rioux, Punya Nachappa, Shelby Ellison
PhytoFrontiers™.2024; 4(4): 524. CrossRef
Efficacy of a membrane concentration method combined with real-time PCR for detection of Giardia and Cryptosporidium in drinking water
Jiang Jingyi, Yao Ping, Xu Jian, Chen Jia, Mao Xujian, Li Qiong, Tu Bowen, Wang Fengming
Letters in Applied Microbiology.2023;[Epub] CrossRef
Screening of specific nucleic acid targets for Cronobacter sakazakii and visual detection by loop-mediated isothermal amplification and lateral flow dipstick method in powdered infant formula
Shiqian Fu, Xue Qin, Zhenghui Wang, Xinyan Yang, Sihan Chen, Tao Yang, Haonan Jin, Chaoxin Man, Yujun Jiang
Journal of Dairy Science.2021; 104(5): 5152. CrossRef
Sporadic cyclosporiasis in symptomatic Cuban patients: Confirmation of positive results from conventional diagnostic methods by molecular assay
Luis Enrique Jerez Puebla, Fidel A. Núñez Fernández, Jorge Fraga Nodarse, Iraís Atencio Millán, Iredys Cruz Rodríguez, Lisette Pérez Santos, Yanet Pintos Saavedra, Laura Rodríguez Moreno, Lucy J. Robertson
Diagnostic Microbiology and Infectious Disease.2020; 97(3): 115048. CrossRef
Human cyclosporiasis
Annunziata Giangaspero, Robin B Gasser
The Lancet Infectious Diseases.2019; 19(7): e226. CrossRef
Cyclospora cayetanensis and Cyclosporiasis: An Update
Sonia Almeria, Hediye N. Cinar, Jitender P. Dubey
Microorganisms.2019; 7(9): 317. CrossRef
Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples
Estelle Menu, Charles Mary, Isabelle Toga, Didier Raoult, Stéphane Ranque, Fadi Bittar
BMC Research Notes.2018;[Epub] CrossRef
Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Shin-Hyeong Cho, Jong-Yil Chai, Eun-Hee Shin
The Korean Journal of Parasitology.2018; 56(5): 419. CrossRef
Entamoeba histolytica: an Overview
Nadia A. El-Dib
Current Tropical Medicine Reports.2017; 4(1): 11. CrossRef
Cryptosporidium and Cyclospora Diarrheal Infection in Malnourished Children: a Nutritional Approach
María A. Echevarría, María L. Eva
Current Tropical Medicine Reports.2017; 4(3): 178. CrossRef

12,390 View
260 Download
9 Web of Science
Crossref

Molecular Identification of Cryptosporidium Species from Pet Snakes in Thailand: Benjarat Yimming, Khampee Pattanatanang, Pornchai Sanyathitiseree, Tawin Inpankaew, Ketsarin Kamyingkird, Nongnuch Pinyopanuwat, Wissanuwat Chimnoi, Jumnongjit Phasuk; Korean J Parasitol 2016;54(4):423-429.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.423

Abstract
PDF

Cryptosporidium is an important pathogen causing gastrointestinal disease in snakes and is distributed worldwide. The main
objective
s of this study were to detect and identify Cryptosporidium species in captive snakes from exotic pet shops and snake farms in Thailand. In total, 165 fecal samples were examined from 8 snake species, boa constrictor (Boa constrictor constrictor), corn snake (Elaphe guttata), ball python (Python regius), milk snake (Lampropeltis triangulum), king snake (Lampropeltis getula), rock python (Python sebae), rainbow boa (Epicrates cenchria), and carpet python (Morelia spilota). Cryptosporidium oocysts were examined using the dimethyl sulfoxide (DMSO)-modified acid-fast staining and a molecular method based on nested-PCR, PCR-RFLP analysis, and sequencing amplification of the SSU rRNA gene. DMSO-modified acid-fast staining revealed the presence of Cryptosporidium oocysts in 12 out of 165 (7.3%) samples, whereas PCR produced positive results in 40 (24.2%) samples. Molecular characterization indicated the presence of Cryptosporidium parvum (mouse genotype) as the most common species in 24 samples (60%) from 5 species of snake followed by Cryptosporidium serpentis in 9 samples (22.5%) from 2 species of snake and Cryptosporidium muris in 3 samples (7.5%) from P. regius.

Citations

Citations to this article as recorded by

Cryptosporidium spp. in reptiles: Detection challenges, molecular characterization and zoonotic risk
Mariana Louro, Laura Hernandez, João Antunes, Luís Madeira de Carvalho, Isabel Pereira da Fonseca, Jacinto Gomes
Food and Waterborne Parasitology.2025; 40: e00272. CrossRef
Minimal zoonotic risk of cryptosporidiosis and giardiasis from frogs and reptiles
Siobhon Egan, Amanda D. Barbosa, Yaoyu Feng, Lihua Xiao, Una Ryan
European Journal of Protistology.2024; 93: 126066. CrossRef
Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms
Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang
Parasite.2024; 31: 47. CrossRef
Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing
Yongru Zeng, Yi Xiong, Chunsheng Yang, Nan He, Jiasong He, Wenxian Luo, Yaohuan Chen, Xiaochen Zeng, Zhengjun Wu
Animals.2022; 12(20): 2726. CrossRef
Prevalence and Molecular Characterization of Cryptosporidium serpentis in Captive Snakes in China
Yijun Chai, Haifeng Liu, Lei Deng, Bo Bi, Jingxin Yao, Xingtao Yang, Zhijun Zhong, Hualin Fu, Liuhong Shen, Ziyao Zhou, Yi Geng, Guangneng Peng
Journal of Parasitology.2021;[Epub] CrossRef
Evaluation of Paromomycin Treatment for Cryptosporidium serpentis Infection in Eastern Indigo Snakes (Drymarchon couperi)
James E. Bogan, Michelle Hoffman, Falicia Dickerson, Mark A. Mitchell, Michael M. Garner, April Childress, James F.X. Wellehan
Journal of Herpetological Medicine and Surgery.2021;[Epub] CrossRef
Detection and molecular characterization of Cryptosporidium species in wild-caught pet spiny-tailed lizards
Rie Kubota, Toshihiro Tokiwa, Katsuki Matsubara, Minoru Okamoto, Kazunori Ike
International Journal for Parasitology: Parasites and Wildlife.2020; 11: 83. CrossRef
The first genetic assessment of wild and farmed ball pythons (Reptilia, Serpentes, Pythonidae) in southern Togo
Mark Auliya, Sylvia Hofmann, Gabriel H. Segniagbeto, Délagnon Assou, Delphine Ronfot, Jonas J. Astrin, Sophia Forat, Guillaume Koffivi K. Ketoh, Neil D’Cruze
Nature Conservation.2020; 38: 37. CrossRef
Molecular identification of Cryptosporidium spp. in pet snakes in Beijing, China
Haixia Zhang, Zixiang Lin, Yuxi Jiang, Weifeng Qian, Chaochao Lv, Liwei Zhang, Siqi Wang, Meng Qi, Zhaofei Xia
Parasitology Research.2020; 119(9): 3119. CrossRef
Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Xiao Xiao, Rui Qi, Hui-Ju Han, Jian-Wei Liu, Xiang-Rong Qin, Li-Zhu Fang, Chuan-Min Zhou, Xiao-Qing Gong, Si-Cong Lei, Xue-Jie Yu
International Journal for Parasitology: Parasites and Wildlife.2019; 10: 274. CrossRef
Gastric Cryptosporidiosis in Snakes, a Review
James E. Bogan
Journal of Herpetological Medicine and Surgery.2019; 29(3-4): 71. CrossRef

10,818 View
222 Download
14 Web of Science
Crossref

Characteristics of Imported Malaria and Species of Plasmodium Involved in Shandong Province, China (2012-2014): Chao Xu, Qing-Kuan Wei, Jin Li, Ting Xiao, Kun Yin, Chang-Lei Zhao, Yong-Bin Wang, Xiang-Li Kong, Gui-Hua Zhao, Hui Sun, Xin Liu, Bing-Cheng Huang; Korean J Parasitol 2016;54(4):407-414.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.407

Abstract
PDF

Malaria remains a serious public health problem in Shandong Province, China; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. In this study, data of malaria cases reported in Shandong during 2012-2014 were analyzed, and Plasmodium species were confirmed by smear microscopy and nested-PCR. A total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. Of all cases, P. falciparum was dominant (81.3%), followed by P. vivax (11.8%); P. ovale and P. malariae together accounted for 6.4% of cases. Notably, for the first time since 2012, no indigenous case had been reported in Shandong Province, a situation that continued through 2014. Total 95.2% of cases were imported from Africa. The ratio of male/female was 92.5:1, and 96.8% of cases occurred in people 20-54 years of age. Farmers or laborers represented 77.5% of cases. No significant trends of monthly pattern were found in the reported cases. All patients were in good condition after treatment, except for 3 who died. These results indicate that imported malaria has increased significantly since 2012 in Shandong Province, especially for P. falciparum, and there is an emergence of species diversity.

Citations

Citations to this article as recorded by

Toward the Elimination of Malaria in China: A Retrospective Analysis of Malaria-Endemic Characteristics and Prevention Effects in Yantai, Shandong Province, 1951 to 2017
Xiao Song, Yang Wang, Xiangli Kong, Haifang Wang, Xiaodan Huang, Hongmei Liu, Lijuan Liu, Xiuxia Guo, Chongxing Zhang, Yuqiang Zhao, Jingxuan Kou, Huaiwei Wang, Peng Cheng, Maoqing Gong
Vector-Borne and Zoonotic Diseases.2020; 20(3): 197. CrossRef
The impact of imported malaria by gold miners in Roraima: characterizing the spatial dynamics of autochthonous and imported malaria in an urban region of Boa Vista
Jaime Louzada, Nathália Coelho Vargas de Almeida, Joao Luiz Pereira de Araujo, Júlio Silva, Thiago M Carvalho, Ananias A Escalante, Joseli Oliveira-Ferreira
Memórias do Instituto Oswaldo Cruz.2020;[Epub] CrossRef
Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018
Yiting Xie, Kai Wu, Weijia Cheng, Tingting Jiang, Yi Yao, Mingxing Xu, Yan Yang, Huabing Tan, Jian Li
Malaria Journal.2020;[Epub] CrossRef
Misidentification of Plasmodium ovale as Plasmodium vivax malaria by a microscopic method: a meta-analysis of confirmed P. ovale cases
Manas Kotepui, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Giovanni De Jesus Milanez
Scientific Reports.2020;[Epub] CrossRef
Monitoring and evaluation of intervals from onset of fever to diagnosis before “1-3-7” approach in malaria elimination: a retrospective study in Shanxi Province, China from 2013 to 2018
Ting Wang, Shui-Sen Zhou, Jun Feng, Myo Minn Oo, Jing Chen, Chang-Fu Yan, Yi Zhang, Ping Tie
Malaria Journal.2019;[Epub] CrossRef
Surveillance of Antimalarial Resistance Pfcrt, Pfmdr1, and Pfkelch13 Polymorphisms in African Plasmodium falciparum imported to Shandong Province, China
Chao Xu, Qingkuan Wei, Kun Yin, Hui Sun, Jin Li, Ting Xiao, Xiangli Kong, Yongbin Wang, Guihua Zhao, Song Zhu, Jingxuan Kou, Ge Yan, Bingcheng Huang
Scientific Reports.2018;[Epub] CrossRef
Epidemiological analysis of 133 malaria cases in Shanxian county, Shandong Province, China
Qi-Qi Shi, Peng Cheng, Chong-Xing Zhang, Xiu-Xia Guo, Li-Juan Liu, Hai-Fang Wang, Jing-Xuan Kou, Xiao-Dan Huang, Huai-Wei Wang, Mao-Qing Gong
Asian Pacific Journal of Tropical Medicine.2017; 10(8): 802. CrossRef

11,204 View
150 Download
8 Web of Science
Crossref

Hemozoin Pigment: An Important Tool for Low Parasitemic Malarial Diagnosis: Sarita Mohapatra, Arnab Ghosh, Ruchi Singh, Dhirendra Pratap Singh, Bhawna Sharma, Jyotish Chandra Samantaray, Manorama Deb, Rajni Gaind; Korean J Parasitol 2016;54(4):393-397.
Published online August 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.4.393

Abstract
PDF

Low parasitemic condition in malaria remains a diagnostic challenge; as the available diagnostic methods failed to detect. Currently, hemozoin (Hz) pigment is gaining attention in the diagnosis of malaria. The major drawback is ease of detection of Hz in routine practice. A pilot study was conducted to evaluate the role of Hz pigment and to compare the performance of quantitative buffy coat assay (QBC) and PCR in such conditions. Clinically suspected cases of malaria were examined by both Giemsa stain and immunochromatographic test (ICT). Samples positive by ICT and negative by Giemsa stain were further examined by nested PCR targeting 18S rRNA and QBC for the presence of malaria parasites and pigments. Thirty blood samples fulfilled the inclusion criteria out of which 23 were Plasmodium vivax (Pv), 4 Plasmodium falciparum (Pf), and 3 mixed (Pv and Pf) by immunochromatographic test. Twenty-one out of 30 (70%) were positive by nested PCR in comparison to 25/30 (83%) by QBC. Samples containing both malaria parasites and Hz pigment by QBC completely showed concordance with the PCR result. However, 61% of total samples containing only Hz pigment were observed positive by PCR. Hz pigment remains an important tool for malaria diagnosis. Identification of leukocytes containing pigments by QBC not only indicates recent malarial infections but also puts light on severity of the disease. QBC assay is a rapid, highly sensitive, and cost-effective method to detect malaria parasites and Hz pigment especially in low parasitemic conditions.

Citations

Citations to this article as recorded by

Exploring Biomarkers for Malaria: Advances in Early Detection and Asymptomatic Diagnosis
Jacko Abiwaqash Harmonis, Sri Agung Fitri Kusuma, Yaya Rukayadi, Aliya Nur Hasanah
Biosensors.2025; 15(2): 106. CrossRef
The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas
Adriana Calderaro, Giovanna Piccolo, Carlo Chezzi
International Journal of Molecular Sciences.2024; 25(2): 695. CrossRef
Hematin anhydride (β-hematin): An analogue to malaria pigment hemozoin possesses nonlinearity
Priyadarshi Sahoo, Nitesh Kumar Pathak, D. Scott Bohle, Erin L. Dodd, Umakanta Tripathy
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2024; 310: 123902. CrossRef
Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon
Aayire C. Yadem, Jillian N. Armstrong, Mustafa Sarimollaoglu, Civian Kiki Massa, Jean-Michel Ndifo, Yulian A. Menyaev, Anastasie Mbe, Kacey Richards, Martina Wade, Yushun Zeng, Ruimin Chen, Qifa Zhou, Elvis Meten, Rodrigue Ntone, Yves Le Grand Napa Tchued
Nature Communications.2024;[Epub] CrossRef
Recent progress in electrochemical sensors for detection and quantification of malaria
Zondi Nate, Atal A.S. Gill, Ruchika Chauhan, Rajshekhar Karpoormath
Analytical Biochemistry.2022; 643: 114592. CrossRef
Review of Microdevices for Hemozoin-Based Malaria Detection
Vitória Baptista, Weng Kung Peng, Graça Minas, Maria Isabel Veiga, Susana O. Catarino
Biosensors.2022; 12(2): 110. CrossRef
Enhancing the sensitivity of micro magnetic resonance relaxometry detection of low parasitemia Plasmodium falciparum in human blood
Smitha Surendran Thamarath, Aoli Xiong, Po-Han Lin, Peter Rainer Preiser, Jongyoon Han
Scientific Reports.2019;[Epub] CrossRef
Comparación entre metodologías para el diagnóstico microscópico de malaria
Liliana Jazmín Cortés, Lyda Muñoz, Martha Stella Ayala
Biomédica.2018; 38(2): 244. CrossRef

12,780 View
230 Download
11 Web of Science
Crossref

Brief Communications

Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran: Mahmoud Agholi, Shahrbanou Naderi Shahabadi, Mohammad Hossein Motazedian, Gholam Reza Hatam; Korean J Parasitol 2016;54(3):339-344.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.339

Abstract
PDF

The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.

Citations

Citations to this article as recorded by

Simultaneous Detection of Sarcocystis hominis, S. heydorni, and S. sigmoideus in Human Intestinal Sarcocystosis, France, 2021–2024
Maxime Moniot, Patricia Combes, Damien Costa, Nicolas Argy, Marie-Fleur Durieux, Thomas Nicol, Céline Nourrisson, Philippe Poirier
Emerging Infectious Diseases.2025;[Epub] CrossRef
Unveiling the hidden threat: prevalence of Enterocytozoon bieneusi and other intestinal parasitic infections among organ transplant recipients in Southern Iran
Mohammad Hossein Motazedian, Leila Morabi, Shahrokh Ezzatzadegan Jahromi, Mojtaba Shafiekhani, Saeed Shahabi, Hamed Nikoupour Dailami, Siavash Gholami, Bahador Sarkari
Gut Pathogens.2025;[Epub] CrossRef
Molecular characterisation of sarcosporidiosis in cattle of Duhok governorate in Kurdistan region of Iraq
S. N. Hussein, M. S. Shukur, A. A. Ibrahim
BULGARIAN JOURNAL OF VETERINARY MEDICINE.2025; 28(2): 210. CrossRef
Worldwide Epidemiology of Cyclospora cayetanensis in HIV/AIDS Patients: A Systematic Review and Meta-Analysis
Farzad Mahdavi, Ali Pouryousef, Mohammad Reza Mohammadi, Kambiz Karimi, Asma Mousivand, Farajolah Maleki, Ali Asghari, Mina Mamizadeh
Acta Parasitologica.2025;[Epub] CrossRef
Prevalence and Associated Risk Factors of Cyclospora cayetanensis in Immunocompromised Patients: A Systematic Review and Meta‐Analysis
Ali Ghorbani, Rostam Menati, Farshad Kakian, Ali Pouryousef, Asma Mousivand, Kambiz Karimi, Farajolah Maleki, Ali Asghari, Jalil Feizi, Panagiotis Karanis
Canadian Journal of Infectious Diseases and Medical Microbiology.2025;[Epub] CrossRef
Infectious etiologies of persistent and chronic diarrhea in Asian developing countries: A systematic review and meta‐analysis
Matthew Chung Yi Koh, Shi Ni Tan, Jinghao Nicholas Ngiam, Paul Anantharajah Tambyah, Kewin Tien Ho Siah
Journal of Gastroenterology and Hepatology.2024; 39(9): 1760. CrossRef
Microscopic and Molecular Identification of Cyclospora cayetanensis and Cystoisospora belli in HIV-Infected People in Tabriz, Northwest of Iran
Saba Ramezanzadeh, Gholamreza Barzegar, Hamid Owaysee Osquee, Majid Pirestani, Mahmoud Mahami-Oskouei, Maryam Hajizadeh, Seyed Abdollah Hosseini, Sonia M. Rodrigues Oliveira, Mahmoud Agholi, Maria de Lourdes Pereira, Ehsan Ahmadpour
Tropical Medicine and Infectious Disease.2023; 8(7): 368. CrossRef
Prevalence of Intestinal Parasitic Diseases in HIV Patients in Tabriz(Original article)
Saba Ramezanzadeh, Golam Reza Barzegar, Hamid Oveisi Oskouei, Majid Pirestani, Mahmoud Mahami-Oskouei, Farzaneh Jafarian, Ehsan Ahmadpour, Seyed Abdollah Hosseini
Journal of Ardabil University of Medical Sciences.2023; 23(4): 352. CrossRef
Global Burden of Cyclospora cayetanensis Infection and Associated Risk Factors in People Living with HIV and/or AIDS
Saba Ramezanzadeh, Apostolos Beloukas, Abdol Sattar Pagheh, Mohammad Taghi Rahimi, Seyed Abdollah Hosseini, Sonia M. Rodrigues Oliveira, Maria de Lourdes Pereira, Ehsan Ahmadpour
Viruses.2022; 14(6): 1279. CrossRef
Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq
Shadan Hassan Abdullah
Veterinary World.2021; 14(2): 468. CrossRef
Prevalence of intestinal microsporidiosis by morphological and molecular methods in eastern Iran
Mahmoodreza Behravan, Mustapha Ahmed Yusuf, Rahmat Solgi, Ali Haghighi
Gene Reports.2021; 24: 101296. CrossRef
Comparison of Two Real-Time PCR Assays Targeting Ribosomal Sequences for the Identification of Cystoisospora belli in Human Stool Samples
Martin Blohm, Andreas Hahn, Ralf Matthias Hagen, Kirsten Alexandra Eberhardt, Holger Rohde, Gérard Leboulle, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt
Pathogens.2021; 10(8): 1053. CrossRef
Molecular differentiation of cattle Sarcocystis spp. by multiplex PCR targeting 18S and COI genes following identification of Sarcocystis hominis in human stool samples
S. Rubiola, T. Civera, E. Ferroglio, S. Zanet, T. Zaccaria, S. Brossa, R. Cipriani, F. Chiesa
Food and Waterborne Parasitology.2020; 18: e00074. CrossRef
Sarcocystosis in Ruminants of Iran, as Neglected Food-Borne Disease: A Systematic Review and Meta-analysis
Davood Anvari, Elahe Narouei, Mona Hosseini, Mohammad Reza Narouei, Ahmad Daryani, Seyyed Ali Shariatzadeh, Abdol Sattar Pagheh, Shirzad Gholami, Shahabeddin Sarvi, Dariush Sargazi, Reza Saberi, Seyed Abdollah Hosseini, Abolghasem Siyadatpanah
Acta Parasitologica.2020; 65(3): 555. CrossRef
Cystoisospora belliinfections in humans: the past 100 years
J. P. Dubey, S. Almeria
Parasitology.2019; 146(12): 1490. CrossRef
Sarcocystis spp. in Romanian Slaughtered Cattle: Molecular Characterization and Epidemiological Significance of the Findings
Kálmán Imre, Gheorghe Dărăbuș, Emil Tîrziu, Sorin Morariu, Mirela Imre, Judit Plutzer, Marius V. Boldea, Adriana Morar
BioMed Research International.2019; 2019: 1. CrossRef
First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt
Asmaa M. El-kady, Nermean M. Hussein, Amal A. Hassan
Journal of Parasitic Diseases.2018; 42(1): 114. CrossRef
Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Seobo Sim, Jua Won, Jae-Whan Kim, Kyungjin Kim, Woo-Yoon Park, Jae-Ran Yu
The Korean Journal of Parasitology.2017; 55(2): 137. CrossRef
Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran
Mahmoud Rahdar, Tahereh Kardooni
Jundishapur Journal of Microbiology.2017;[Epub] CrossRef
Human intestinal sarcocystosis in Iran: there but not seen
Mahmoud Agholi, Zahra Taghadosi, Davood Mehrabani, Farzaneh Zahabiun, Zahra Sharafi, Mohammad Hossein Motazedian, Gholam Reza Hatam, Shahrbanou Naderi Shahabadi
Parasitology Research.2016; 115(12): 4527. CrossRef

12,321 View
149 Download
20 Web of Science
Crossref

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(3):329-334.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.329

Abstract
PDF

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

Citations

Citations to this article as recorded by

Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson
Microbial Biotechnology.2025;[Epub] CrossRef
A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis
Zhenke Yang, Jinghui Wang, Yiming Qi, Yiping Shi, Fakun Li, Weijuan Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang
Parasites & Vectors.2024;[Epub] CrossRef
A novel fluoro colorimetric Loop mediated isothermal amplification (LAMP) assay for detection of Trichomonas vaginalis
Shoorashetty Manohar Rudresh, Pareyam Pooja, Pattacheravanda Nanaiah Shakuntala, Kanta Madhu
Indian Journal of Medical Microbiology.2024; 49: 100610. CrossRef
Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen
Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang
Acta Tropica.2024; 260: 107489. CrossRef
Label-free electrochemical DNA biosensing of MR TV 29 18s ribosomal RNA gene of Trichomonas vaginalis by signalization of non-spherical gold nanoparticles
R. Dehdari Vais, H. Heli, N. Sattarahmady
Materials Today Communications.2023; 34: 105123. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
A novel and ultrasensitive label-free electrochemical DNA biosensor for Trichomonas vaginalis detection based on a nanostructured film of poly(ortho-aminophenol)
Rezvan Dehdari Vais, Hossein Heli, Naghmeh Sattarahmady, Afshin Barazesh
Synthetic Metals.2022; 287: 117082. CrossRef
Omics Analyses of Trichomonas vaginalis Actin and Tubulin and Their Participation in Intercellular Interactions and Cytokinesis
Sebastián Lorenzo-Benito, Luis Alberto Rivera-Rivas, Lizbeth Sánchez-Ayala, Jaime Ortega-López, Octavio Montes-Flores, Daniel Talamás-Lara, Rossana Arroyo
Genes.2022; 13(6): 1067. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA
S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady
Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Loop mediated isothermal amplification assay for detection of Trichomonas vaginalis in vaginal swabs among symptomatic women from North India
S. Khurana, R. Dadwal, N. Sharma, A. Mewara, S. Singh, R. Bagga, R. Yadav, S. Sethi
Letters in Applied Microbiology.2020; 70(3): 196. CrossRef
Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Junjun Zhai, Zhang Yan, Feng Ping, Qu Lei, Xuelong Chen, Yanping Qi, Tianwen Wang
PLOS ONE.2020; 15(4): e0230881. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Label-free ultrasensitive electrochemical genosensing of Trichomonas vaginalis using anisotropic-shaped gold nanoparticles as a platform, a repeated sequence of the parasite DNA as a probe, and toluidine blue as a redox marker
N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. Heli
Sensors and Actuators B: Chemical.2018; 273: 234. CrossRef
Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
Shao-Xin Cai, Fan-De Kong, Shu-Fei Xu, Cui-Luan Yao
PeerJ.2018; 6: e5993. CrossRef

10,601 View
196 Download
15 Web of Science
Crossref

Case Report

Painless Jaundice Caused by Clonorchis sinensis Infection: A Case Report: Yuhua Hao, Wanguo Bao, Meishan Jin, Yuxiang Li, Feng Wang; Korean J Parasitol 2016;54(3):323-327.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.323

Abstract
PDF

A man with only yellowing of the skin and eye sclera was diagnosed with clonorchiasis, which rarely manifested jaundice as the initial symptom. However, because of a lack of evidence for a diagnostic gold standard, the time until definitive diagnosis was more than a week. The diagnostic process relied on inquiring about the patient’s history, including the place of residence, dietary habits, and symptoms, as well as on serological findings, an imaging examination, and pathological findings. MRCP and CT results showed mild dilatation of intrahepatic ducts and increased periductal echogenicity. The eggs were ultimately found in stool by water sedimentation method after the negative report through direct smear. DNA sequencing of PCR production of the eggs demonstrated 98-100% homology with ITS2 of Clonorchis sinensis. After anti-parasite medical treatment, the patient’s symptoms were gradually relieved. Throughout the diagnostic procedure, besides routine examinations, the sedimentation method or concentration method could be used as a sensitive way for both light and heavy C. sinensis infection in the definite diagnosis.

Citations

Citations to this article as recorded by

Research and Discussion on the Clinical Diagnosis Methods of Human Clonorchis sinensis
建锌陈
Hans Journal of Biomedicine.2025; 15(02): 273. CrossRef
Novel mechanism of hepatobiliary system damage and immunoglobulin G4 elevation caused by Clonorchis sinensis infection
Xin-He Zhang, Die Huang, Yi-Ling Li, Bing Chang
World Journal of Clinical Cases.2021; 9(23): 6639. CrossRef
Surgical Infections in Low- and Middle-Income Countries: A Global Assessment of the Burden and Management Needs
Jennifer Rickard, Gregory Beilman, Joseph Forrester, Robert Sawyer, Andrew Stephen, Thomas G. Weiser, Julie Valenzuela
Surgical Infections.2020; 21(6): 478. CrossRef
Clonorchiasis sinensis detected by laparoscopic exploration of biliary tracts in two patients with obstructive jaundice
Xialei Liu, Genglong Zhu, Chaonong Cai, Zhiyue Lv, Jian Li
BMC Infectious Diseases.2019;[Epub] CrossRef
Acute shock caused by Clonorchis sinensis infection: a case report
Nan Wang, Bin Tang, Yuhua Hao, Xue Bai, Xuelin Wang, Yuxiang Li, Yong Yang, Shicun Li, Shuo Hao, Xinyu Wang, Mingyuan Liu, Xiaolei Liu
BMC Infectious Diseases.2019;[Epub] CrossRef

12,230 View
152 Download
4 Web of Science
Crossref

Original Articles

Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate: Ahmed K. Dyab, Lamia A. Galal, Abeer E. Mahmoud, Yasser Mokhtar; Korean J Parasitol 2016;54(3):265-272.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.265

Abstract
PDF

Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for worm survival and reproduction. In Egypt, no available data were found about Wolbachia searching for it in either mosquitoes or filarial worms. Thus, we aimed to identify the possible concurrent presence of Wolbachia within different mosquitoes and filarial parasites, in Assiut Governorate, Egypt using multiplex PCR. Initially, 6 pools were detected positive for Wolbachia by single PCR. The simultaneous detection of Wolbachia and filarial parasites (Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens) by multiplex PCR was spotted in 5 out of 6 pools, with an overall estimated rate of infection (ERI) of 0.24%. Unexpectedly, the highest ERI (0.53%) was for Anopheles pharoensis with related Wolbachia and W. bancrofti, followed by Aedes (0.42%) and Culex (0.26%). We also observed that Wolbachia altered Culex spp. as a primary vector for W. bancrofti to be replaced by Anopheles sp. Wolbachia within filaria-infected mosquitoes in our locality gives a hope to use bacteria as a new control trend simultaneously targeting the vector and filarial parasites.

Citations

Citations to this article as recorded by

Distinct microbial communities of drain flies (Clogmia albipunctata) across sites with differing human influence
Hyun Seo Park, Xavier Chavarria, Arwa Shatta, Dongjun Kang, Singeun Oh, Du-Yeol Choi, Jun Ho Choi, Myungjun Kim, Yoon Hee Cho, Myung-hee Yi, Ju Yeong Kim
FEMS Microbiology Letters.2025;[Epub] CrossRef
Mitochondrial and microbial diversity of the invasive mosquito vector species Culex tritaeniorhynchus across its extensive inter-continental geographic range
Claire L. Jeffries, Luciano M Tantely, Perparim Kadriaj, Marcus S C Blagrove, Ioanna Lytra, James Orsborne, Hasan Mohammad Al-Amin, Abdul Rahim Mohammed, Mohammad Shafiul Alam, Romain Girod, Yaw A Afrane, Silvia Bino, Vincent Robert, Sebastien Boyer, Matt
Wellcome Open Research.2024; 9: 18. CrossRef
Culex-Transmitted Diseases: Mechanisms, Impact, and Future Control Strategies using Wolbachia
Mukund Madhav, Kim R. Blasdell, Brendan Trewin, Prasad N. Paradkar, Adam J. López-Denman
Viruses.2024; 16(7): 1134. CrossRef
Mosquito Vectors (Diptera: Culicidae) and Mosquito-Borne Diseases in North Africa
Amira Nebbak, Lionel Almeras, Philippe Parola, Idir Bitam
Insects.2022; 13(10): 962. CrossRef
Larvicidal and repellent potential of Ageratum houstonianum against Culex pipiens
Doaa El Hadidy, Abeer M. El Sayed, Mona El Tantawy, Taha El Alfy, Shaimaa M. Farag, Doaa R. Abdel Haleem
Scientific Reports.2022;[Epub] CrossRef
Wolbachia infection in West Nile Virus vectors of northwest Iran
Faramarz Bozorg-Omid, Mohammad Ali Oshaghi, Mozaffar Vahedi, Fateh Karimian, Seyyed Javad Seyyed-Zadeh, Ali Reza Chavshin
Applied Entomology and Zoology.2020; 55(1): 105. CrossRef
Dirofilaria and Wolbachia in mosquitoes (Diptera: Culicidae) in central European Russia and on the Black Sea coast
Elena Shaikevich, Anna Bogacheva, Ludmila Ganushkina
Parasite.2019; 26: 2. CrossRef

11,443 View
153 Download
8 Web of Science
Crossref

Effective High-Throughput Blood Pooling Strategy before DNA Extraction for Detection of Malaria in Low-Transmission Settings: Myat Htut Nyunt, Myat Phone Kyaw, Kyaw Zin Thant, Thinzer Shein, Soe Soe Han, Ni Ni Zaw, Jin-Hee Han, Seong-Kyun Lee, Fauzi Muh, Jung-Yeon Kim, Shin-Hyeong Cho, Sang-Eun Lee, Eun-Jeong Yang, Chulhun L. Chang, Eun-Taek Han; Korean J Parasitol 2016;54(3):253-259.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.253

Abstract
PDF

In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost- and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 ?l in 1 sample, was optimal, and the parasite density as low as 2 p/?l for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings (<9% positive rate). The results indicated that pooling of the blood samples before DNA extraction followed by usual nested PCR is useful and effective for detection of malaria in screening of hidden cases in low-transmission settings.

Citations

Citations to this article as recorded by

Assessing the potential for specimen pooling to streamline nosocomial surveillance of methicillin-resistant Staphylococcus aureus (MRSA)
Isabella Pagotto, Mohammed Alqahtani, Bryn Joy, Gregory R. McCracken, Ian R. Davis, Jason J. LeBlanc, Glenn Patriquin, Wendy A. Szymczak
Microbiology Spectrum.2025;[Epub] CrossRef
A sequential decoding procedure for pooled quantitative measure
Yunning Zhong, Ping Xu, Siming Zhong, Juan Ding
Sequential Analysis.2022; 41(1): 68. CrossRef
Surveillance on the Vivax Malaria in Endemic Areas in the Republic of Korea Based on Molecular and Serological Analyses
Seong-Kyun Lee, Fengyue Hu, Egy Rahman Firdaus, Ji-Hoon Park, Jin-Hee Han, Sang-Eun Lee, Hyun-Il Shin, Shin Hyeong Cho, Won Sun Park, Feng Lu, Eun-Taek Han
The Korean Journal of Parasitology.2020; 58(6): 609. CrossRef
Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar
Myat Htut Nyunt, Than Naing Soe, Thinzar Shein, Ni Ni Zaw, Soe Soe Han, Fauzi Muh, Seong-Kyun Lee, Jin-Hee Han, Ji-Hoon Park, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Myat Phone Kyaw, Eun-Taek Han
Malaria Journal.2018;[Epub] CrossRef
Current Status of Standard Diagnostics and Treatment for Malaria, Tuberculosis, and Hepatitis in Myanmar
Eun-Taek Han, Jong-Seok Lee, Jae-Hun Cheong, Chulhun L. Chang, Myat Htut Nyunt, Wah Wah Aung, Yi Yi Kyaw, Kyaw Zin Thant
Laboratory Medicine Online.2017; 7(3): 94. CrossRef
Optimization and evaluation of the qPCR-based pooling strategy DEP-pooling in dairy production for the detection of Listeria monocytogenes
Patrick Mester, Anna Kristina Witte, Christian Robben, Eva Streit, Susanne Fister, Dagmar Schoder, Peter Rossmanith
Food Control.2017; 82: 298. CrossRef

12,301 View
162 Download
5 Web of Science
Crossref

Brief Communications

Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea: Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu; Korean J Parasitol 2016;54(2):187-190.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.187

Abstract
PDF

Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50.

Citations

Citations to this article as recorded by

Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran
Maryam Alikhani, Mohammad Akhoundi, Denis Sereno, Jahangir Abdi, Razi Naserifar, Mohammad Reza Mahmoudi, Asad Mirzaei
Parasitology Research.2022; 121(6): 1631. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef

8,713 View
105 Download
5 Web of Science
Crossref

Serological Detection of Borrelia burgdorferi among Horses in Korea: Seung-Hun Lee, Sun-Hee Yun, Eunsang Choi, Yong-Soo Park, Sang-Eun Lee, Gil-Jae Cho, Oh-Deog Kwon, Dongmi Kwak; Korean J Parasitol 2016;54(1):97-101.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.97

Abstract
PDF

Lyme disease is a tick-borne zoonotic infectious disease caused by Borrelia burgdorferi. The present study assessed the infection status of B. burgdorferi among horses reared in Korea using ELISA and PCR. Between 2009 and 2013, blood samples were collected from 727 horses throughout Korea. Data for each animal including age, gender, breed, and region of sample collection were used for epidemiological analysis. Overall, 38 (5.2%; true prevalence: 5.5%) of 727 horses were seropositive by ELISA. There were statistically significant differences according to breed and region (P<0.001) whose differences might be attributed to the ecology of vector ticks and climate conditions. Using 2 nested PCR, none of the samples tested positive for B. burgdorferi. Thus, a positive ELISA result can indicate only that the tested horse was previously exposed to B. burgdorferi, with no certainty over the time of exposure. Since global warming is likely to increase the abundance of ticks in Korea, continuous monitoring of tick-borne diseases in Korean horses is needed.

Citations

Citations to this article as recorded by

First Detection of Seroreactivity to Lyme Borreliosis in Agricultural Workers from a Non-Endemic Region of South Korea
Jeong Rae Yoo, Miyeon Kim, Wooseong Jeong, Sung Wook Song
Yonsei Medical Journal.2025; 66(7): 456. CrossRef
Molecular surveillance of zoonotic pathogens from wild rodents in the Republic of Korea
Kyoung-Seong Choi, Sunwoo Hwang, Myung Cheol Kim, Hyung-Chul Cho, Yu-Jin Park, Min-Jeong Ji, Sun-Woo Han, Joon-Seok Chae, Colleen B. Jonsson
PLOS Neglected Tropical Diseases.2024; 18(7): e0012306. CrossRef
Surveillance of Vector‐Borne Zoonotic Diseases in South Korea: Uncovering Novel Pathogen Carriers Among Rodents and Mites Nationwide
Beoul Kim, You-Jeong Lee, Hee Il Lee, Dongmi Kwak, Min-Goo Seo, Nan-hua Chen
Transboundary and Emerging Diseases.2024;[Epub] CrossRef
Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses
Heidrun Gehlen, Katharina Inerle, Alexander Bartel, Sabita Diana Stöckle, Sebastian Ulrich, Beatrice Briese, Reinhard K. Straubinger
Animals.2023; 13(12): 1984. CrossRef
Antibodies against Borrelia burgdorferi Sensu Lato in Clinically Healthy and Sick Horses: First Report from the Czech Republic
Nikola Kašpárková, Eva Bártová, Alena Žákovská, Marie Budíková, Kamil Sedlák
Microorganisms.2023; 11(7): 1706. CrossRef
Surveillance and Molecular Identification ofBorreliaSpecies in Ticks Collected at U.S. Army Garrison Humphreys, Republic of Korea, 2018–2019
Seung-Ho Lee, Sung-Tae Chong, Heung-Chul Kim, Terry A Klein, Kyungmin Park, Jingyeong Lee, Jeong-Ah Kim, Won-Keun Kim, Jin-Won Song, Sarah Hamer
Journal of Medical Entomology.2022; 59(1): 363. CrossRef
Seroepidemiologic Survey of Lyme Disease among Forestry Workers in National Park Offices in South Korea
Dilaram Acharya, Ji-Hyuk Park
International Journal of Environmental Research and Public Health.2021; 18(6): 2933. CrossRef
Frequency and factors associated of potential zoonotic pathogens (Borrelia spp., Rickettsia spp., Leishmania spp., and Anaplasma phagocytophilum) in equids in the state of Bahia, Brazil
Sonia Carmen Lopo Costa, Jéssica de Souza Freitas, Fábio Santos Carvalho, Maria Julia Salim Pereira, Matheus Dias Cordeiro, Adivaldo Henrique da Fonseca, Márcia Mariza Gomes Jusi, Rosangela Zacarias Machado, Alexandre Dias Munhoz
Parasites & Vectors.2021;[Epub] CrossRef
Detection of Anaplasma Phagocytophilum in Horses With Suspected Tick-Borne Disease in Northeastern United States by Metagenomic Sequencing
Murugan Subbiah, Nagaraja Thirumalapura, David Thompson, Suresh V. Kuchipudi, Bhushan Jayarao, Deepanker Tewari
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea
Hyun-Ji Seo, A-Tai Truong, Keun-Ho Kim, Ji-Yeon Lim, Subin Min, Heung-Chul Kim, Mi-Sun Yoo, Soon-Seek Yoon, Terry A. Klein, Yun Sang Cho
Pathogens.2021; 10(9): 1069. CrossRef
Seroprevalence for Evidence Detection of Borrelia Infection in Dogs

Annals of Veterinary Science.2021; : 1. CrossRef
Molecular Identification of Borrelia afzelii from Ticks Parasitizing Domestic and Wild Animals in South Korea
Min-Goo Seo, Oh-Deog Kwon, Dongmi Kwak
Microorganisms.2020; 8(5): 649. CrossRef
Seroprevalence of antibodies against Anaplasma phagocytophilum and Borrelia burgdorferi in horses (Equus caballus) from northern Algeria
Abdelouahab Laamari, Naouelle Azzag, Safia Tennah, Salima-Yamina Derdour, Bernard China, Ryhan Bouabdallah, Farida Ghalmi
Journal of Veterinary Research.2020; 64(3): 413. CrossRef
Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea
Seong Yoon Kim, Tae-Kyu Kim, Tae Yun Kim, Hee Il Lee
Pathogens.2020; 9(11): 866. CrossRef
Epidemiological Investigation of Tick Species from Near Domestic Animal Farms and Cattle, Goat, and Wild Boar in Korea
Jeong-Byoung Chae, Young-Sun Cho, Yoon-Kyoung Cho, Jun-Gu Kang, Nam-Shik Shin, Joon-Seok Chae
The Korean Journal of Parasitology.2019; 57(3): 319. CrossRef
Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea
Seung-Hun Lee, Youn-Kyoung Goo, Paul John L. Geraldino, Oh-Deog Kwon, Dongmi Kwak
Pathogens.2019; 8(4): 289. CrossRef
Şanlıurfa’da Safkan Arap Atlarında Borrelia burgdorferi Seropozitifliğinin Belirlenmesi
Ömer Demir, Oktay Keskin
Harran Üniversitesi Veteriner Fakültesi Dergisi.2019; 8(2): 221. CrossRef
Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea
Hang Lee, Joon-Seok Chae, Bae-Keun Park, Jinho Park, Do-Hyeon Yu, Jun-Gu Kang, Nam-Shik Shin, Young-Sun Jo, Yoon-Kyoung Cho, Kyoung-Seong Choi, Jeong-Byoung Chae
The American Journal of Tropical Medicine and Hygiene.2018; 98(4): 1061. CrossRef
Horses as a Potential Reservoir of Lyme Borreliosis in Jeju-do, Korea
Jong-Myon Bae
Journal of Preventive Medicine and Public Health.2018; 51(4): 213. CrossRef
Identification of a defined linear epitope in the OspA protein of the Lyme disease spirochetes that elicits bactericidal antibody responses: Implications for vaccine development
Jerilyn R. Izac, Lee D. Oliver, Christopher G. Earnhart, Richard T. Marconi
Vaccine.2017; 35(24): 3178. CrossRef
Borrelia Species Detected in Ticks Feeding on Wild Korean Water Deer (Hydropotes inermis) Using Molecular and Genotypic Analyses
D. VanBik, S. H. Lee, M. G. Seo, B. R. Jeon, Y. K. Goo, S. J. Park, M. H. Rhee, O. D. Kwon, T. H. Kim, P.J.L. Geraldino, D. Kwak
Journal of Medical Entomology.2017; 54(5): 1397. CrossRef

11,018 View
129 Download
20 Web of Science
Crossref

Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(1):75-80.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.75

Abstract
PDF

This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR detected T. vaginalis in 12 (1.9%) patients. Testing via PCR demonstrated high sensitivity and high negative predictive value for T. vaginalis. Among the 19 women who tested positive for T. vaginalis according to PCR, 94.7% (18/19) reported vaginal signs and symptoms. Notably, more than 50% of T. vaginalis infections occurred in females younger than 30 years old, and 58% were unmarried. Multiplex PCR, which simultaneously detects pathogens from various sexually transmitted infections, revealed that 91.7% (11/12) of patients were infected with 2 or more pathogens. Mycoplasma hominis was the most prevalent co-infection pathogen with T. vaginalis, followed by Ureaplasma urealyticum and Chlamydia trachomatis. Our results indicate that PCR and multiplex PCR are the most sensitive tools for T. vaginalis diagnosis, rather than microscopy which has been routinely used to detect T. vaginalis infections in South Korea. Therefore, clinicians should take note of the high prevalence of T. vaginalis infections among adolescent and young women in order to prevent persistent infection and transmission of this disease.

Citations

Citations to this article as recorded by

Diagnostic accuracy of real-time polymerase chain reaction assay for the detection of Trichomonas vaginalis in clinical samples: A systematic review and meta-analysis
Emmanuel O. Babafemi, Benny P. Cherian, Khalid Rahman, Gilbert M. Mogoko, Oluwatoyin O. Abiola
African Journal of Laboratory Medicine.2025;[Epub] CrossRef
The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis
Zeinab Moghadamizad, Javad Yazdizadeh Khalili, Meysam Olfatifar, Milad Badri, Sasan Khazaei
International Health.2024; 16(3): 240. CrossRef
Combination of genitourinary candidiasis and sexually transmitted infections
N.E. Portnyagina, A.K. Kvardakova, V.V. Pakhomova, E.G. Gubanova, N.V. Deeva, I.G. Sergeeva
Russian Journal of Clinical Dermatology and Venereology.2024; 23(4): 377. CrossRef
Trichomonas vaginalis: comparison of primers for implementation as an in-house PCR in rural Vellore, South India
Nagarajan L. Surya, Thangamani Suji, Santhosh Rani, Irene Dorathy, Shantidani Minz, Rani Diana Sahni
BMC Infectious Diseases.2024;[Epub] CrossRef
Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Comparison of Diagnostic Methods for Detection of Trichomonas vaginalis in Prediagnosed Vaginitis Cases and Its Association with Various Pathogens
Vildan Turan Faraşat, İbrahim Cüneyt Balcıoğlu, Pınar Solmaz Hasdemir, Ertaç Gümüş
Turkish Journal of Parasitology.2022; 46(3): 167. CrossRef
Trichomonas vaginalis follow-up and persistence in Colombian women
Lauren Hernández-Buelvas, Milena Camargo, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
Scientific Reports.2021;[Epub] CrossRef
Trichomoniasis in a tertiary hospital of Madrid, Spain (2013–2017): prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis
Celia Bolumburu, Vega Zamora, María Muñoz-Algarra, Francisca Portero-Azorín, José Antonio Escario, Alexandra Ibáñez-Escribano
Parasitology Research.2020; 119(6): 1915. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
PREVALENCE OF TRICHOMONIASIS IN ASYMPTOMATIC PREGNANT WOMEN POPULATION IN BANDUNG, WEST JAVA, INDONESIA
Pati Aji Achdiat, Reiva Farah Dwiyana, Vina Feriza, Rasmia Rowawi, Rendy Ariezal Effendi, Oki Suwarsa, Hendra Gunawan
Indonesian Journal of Tropical and Infectious Disease.2019; 7(4): 57. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(3): 329. CrossRef
Epidemiology of Trichomoniasis in South Korea and Increasing Trend in Incidence, Health Insurance Review and Assessment 2009-2014
So-Young Joo, Youn-Kyoung Goo, Jae-Sook Ryu, Sang-Eun Lee, Won Kee Lee, Dong-Il Chung, Yeonchul Hong, Zhefeng Meng
PLOS ONE.2016; 11(12): e0167938. CrossRef

11,068 View
150 Download
11 Web of Science
Crossref

Original Articles

Validation of Reference Genes for Quantitative Real-Time PCR in Bovine PBMCs Transformed and Non-transformed by Theileria annulata: Hongxi Zhao, Junlong Liu, Youquan Li, Congshan Yang, Shuaiyang Zhao, Juan Liu, Aihong Liu, Guangyuan Liu, Hong Yin, Guiquan Guan, Jianxun Luo; Korean J Parasitol 2016;54(1):39-46.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.39

Abstract
PDF

Theileria annulata is a tick-borne intracellular protozoan parasite that causes tropical theileriosis, a fatal bovine lymphoproliferative disease. The parasite predominantly invades bovine B lymphocytes and macrophages and induces host cell transformation by a mechanism that is not fully comprehended. Analysis of signaling pathways by quantitative real-time PCR (qPCR) could be a highly efficient means to understand this transformation mechanism. However, accurate analysis of qPCR data relies on selection of appropriate reference genes for normalization, yet few papers on T. annulata contain evidence of reference gene validation. We therefore used the geNorm and NormFinder programs to evaluate the stability of 5 candidate reference genes; 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ACTB (β-actin), PRKG1 (protein kinase cGMP-dependent, type I) and TATA box binding protein (TBP). The results showed that 18S rRNA was the reference gene most stably expressed in bovine PBMCs transformed and non-transformed with T. annulata, followed by GAPDH and TBP. While 18S rRNA and GAPDH were the best combination, these 2 genes were chosen as references to study signaling pathways involved in the transformation mechanism of T. annulata.

Citations

Citations to this article as recorded by

Virulence Is More than Adhesion and Invasion Ability, an In Vitro Cell Infection Assay of Bovine Mycoplasma spp.
Elhem Yacoub, Daniel Kos, Murray Jelinski
Microorganisms.2025; 13(3): 632. CrossRef
A Theileria annulata parasite with a single mutation, methionine 128 to isoleucine (M128I), in cytochrome B is resistant to buparvaquone
Shahin Tajeri, Debasish Chattopadhyay, Gordon Langsley, Ard M. Nijhof, Vikrant Sudan
PLOS ONE.2024; 19(4): e0299002. CrossRef
Traditional and emerging Fusarium mycotoxins disrupt homeostasis of bovine mammary cells by altering cell permeability and innate immune function
Ran Xu, Umesh K. Shandilya, Alexandros Yiannikouris, Niel A. Karrow
Animal Nutrition.2023; 12: 388. CrossRef
Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
Melânia Lazzari Rigo, Kauê Rodriguez Martins, Yan Wahast Islabão, Alexia Brauner de Mello, Marjorie de Giacometi, Rodrigo Casquero Cunha, Monique Tomazele Rovani, Camila Belmonte Oliveira
Semina: Ciências Agrárias.2023; 44(6): 2063. CrossRef
1,25‐Dihydroxyvitamin D3 potentiates the innate immune response of peripheral blood mononuclear cells from Japanese Black cattle
Youki Oyamada, Ei'ichi Iizasa, Amane Usa, Konosuke Otomaru
Animal Science Journal.2023;[Epub] CrossRef
Internal reference genes with the potential for normalizing quantitative PCR results for oral fluid specimens
Ting-Yu Cheng, Jeffrey J. Zimmerman, Luis G. Giménez-Lirola
Animal Health Research Reviews.2022; 23(2): 147. CrossRef
Genetic diversity of Siberian bovine coronavirus isolates (Coronaviridae: Coronavirinae: Betacoronavirus-1: Bovine-Like coronaviruses)
Alexander G. Glotov, Aleksej V. Nefedchenko, Anton G. Yuzhakov, Svetlana V. Koteneva, Tatyana I. Glotova, Alina K. Komina, Nikita Yu. Krasnikov
Problems of Virology.2022; 67(6): 465. CrossRef
Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Membranes.2021; 11(12): 933. CrossRef
Inhibition monitoring in veterinary molecular testing
Lifang Yan, Kathy L. Toohey-Kurth, Beate M. Crossley, Jianfa Bai, Amy L. Glaser, Rebecca L. Tallmadge, Laura B. Goodman
Journal of Veterinary Diagnostic Investigation.2020; 32(6): 758. CrossRef
Development and testing of the real-time polymerase chain reaction for identification and quantitative determination of the bovine respiratory syncytial virus
A.V. Nefedchenko, A.G. Glotov, S.V. Koteneva, T.I. Glotova
Molecular Genetics Microbiology and Virology (Russian version).2020; 38(3): 145. CrossRef
Developing and Testing a Real-Time Polymerase Chain Reaction to Identify and Quantify Bovine Respiratory Syncytial Viruses
A. V. Nefedchenko, A. G. Glotov, S. V. Koteneva, T. I. Glotova
Molecular Genetics, Microbiology and Virology.2020; 35(3): 168. CrossRef
Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14/TLR4/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows
B. Stefanska, W. Człapa, E. Pruszynska-Oszmałek, D. Szczepankiewicz, V. Fievez, J. Komisarek, K. Stajek, W. Nowak
Journal of Dairy Science.2018; 101(2): 1297. CrossRef
Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real-time polymerase chain reaction (qPCR)
Teboho N. Tsotetsi, Nicola E. Collins, Marinda C. Oosthuizen, Kgomotso P. Sibeko-Matjila, Gordon Langsley
PLOS ONE.2018; 13(5): e0196715. CrossRef

12,606 View
150 Download
12 Web of Science
Crossref

Isolation and Genotyping of Toxoplasma gondii Strains in Ovine Aborted Fetuses in Khorasan Razavi Province, Iran: Leila Danehchin, Gholamreza Razmi, Abolghasem Naghibi; Korean J Parasitol 2016;54(1):15-20.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.15

Abstract
PDF

Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxolasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged 7-22 ?m. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.

Citations

Citations to this article as recorded by

Unraveling the link: serological and molecular insights into Toxoplasma gondii infection in women with spontaneous abortion history
Narges Arbabi, Nima Firouzeh, Seyed Ghader Azizi, Ahmad Mehravaran, Soudabeh Etemadi, Reza Shafiei, Hadi Mirahmadi
Tropical Diseases, Travel Medicine and Vaccines.2025;[Epub] CrossRef
Is GRA6 Gene a Suitable Marker for Molecular Typing of Toxoplasma Gondii? A Scoping Systematic Review
Rohallah Abedian, Bahman Rahimi Esboei, Mahdi Fakhar, Zahra Rahmani, Mahbobeh Montazeri, Mostafa Solaymani, Hajar Ziaei Hezarjaribi
Acta Parasitologica.2024; 69(1): 175. CrossRef
Serological and molecular survey of Toxoplasma Gondii in aborted livestock fetuses from Northeast Iran
Reza Shafiei, Nima Firouzeh, Mohammad Taghi Rahimi
BMC Research Notes.2024;[Epub] CrossRef
Toxoplasmosis diagnostic techniques: Current developed methods and biosensors
Soheila Molaei, Masoomeh Dadkhah, Farzaneh Fathi
Talanta.2023; 252: 123828. CrossRef
Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran
Shiva Zeinali, Shahram Khademvatan, Rasool Jafari, Shabnam Vazifekhah, Elham Yousefi, Tahereh Behroozi-Lak, Bibi Razieh Hosseini Farash
PLOS ONE.2023; 18(10): e0283493. CrossRef
Investigation of Toxoplasma gondii and Neospora caninum as cause of ovine abortion in affected flocks of Urmia, northwest of Iran
A. Khodadadi, F. Malekifard, R. A. Batavani
BULGARIAN JOURNAL OF VETERINARY MEDICINE.2022; 25(2): 308. CrossRef
Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers
Amin Yousefvand, Seyed Ali Mirhosseini, Masoud Ghorbani, Tahereh Mohammadzadeh, Mehrdad Moosazadeh Moghaddam, Shirin Mohammadyari
Journal of Consumer Protection and Food Safety.2021; 16(2): 117. CrossRef
Global prevalence of Toxoplasma gondii infection in the aborted fetuses and ruminants that had an abortion: A systematic review and meta-analysis
Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Ahmad Daryani
Veterinary Parasitology.2021; 290: 109370. CrossRef
Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh
Tanjila Hasan, Abdul Mannan, Delower Hossain, Azizunnesa Rekha, Md. Monir Hossan, Mohammad Abdul Alim, AHM Musleh Uddin
Veterinary World.2021; : 2386. CrossRef
Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran–Iraq
Arjang Partoandazanpoor, Zainab Sadeghi-Dehkordi, Loghman Ekradi, Monireh Khordadmehr, Maryam Rassouli, Alireza Sazmand
Acta Parasitologica.2020; 65(1): 187. CrossRef
WITHDRAWN: Economic and public health importance of Toxoplasma gondii infections in sheep: the last decade
J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su
Veterinary Parasitology: X.2020; : 100028. CrossRef
Economic and public health importance of Toxoplasma gondii infections in sheep: 2009–2020
J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su
Veterinary Parasitology.2020; 286: 109195. CrossRef
Lack of circulating Toxoplasma gondii DNA in seropositive patients with bipolar or schizophrenia spectrum disorders.
L. Galli, C. Del Grande, L. Rindi, C. Mangia, V. Mangano, E. Schiavi, I. Masci, B. Pinto, L. Kramer, L. Dell'Osso, F. Bruschi
Psychiatry Research.2019;[Epub] CrossRef
Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact
S. Stelzer, W. Basso, J. Benavides Silván, L.M. Ortega-Mora, P. Maksimov, J. Gethmann, F.J. Conraths, G. Schares
Food and Waterborne Parasitology.2019; 15: e00037. CrossRef
Detection of Toxoplasma gondii genotypes in abortion women by RFLP-PCR in Al-Najaf Al-Ashraf province
Saleem Khteer Al-Hadraawy, Mohammad Alzeyadi, Ameer Ali Shakir, Arshad noori Al-dujaili
Journal of Physics: Conference Series.2019; 1234(1): 012084. CrossRef
Molecular genotyping and serological evaluation of Toxoplasma gondii in mothers and their spontaneous aborted fetuses in Southwest of Iran
Nasir Arefkhah, Bahman Pourabbas, Qasem Asgari, Abdolali Moshfe, Fataneh Mikaeili, Gordafarin Nikbakht, Bahador Sarkari
Comparative Immunology, Microbiology and Infectious Diseases.2019; 66: 101342. CrossRef
Serological and molecular detection ofToxoplasma gondiiin sheep and goats in Kashan, Central Iran
Sima Rasti, Nader Marandi, Amir Abdoli, Mahdi Delavari, Seyed Gholam Abbas Mousavi
Journal of Food Safety.2018;[Epub] CrossRef
Prevalence and molecular characterization of Toxoplasma gondii DNA in retail fresh meats in Canada
Asma Iqbal, Nicol Janecko, Frank Pollari, Brent Dixon
Food and Waterborne Parasitology.2018; 13: e00031. CrossRef
Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran
Belal Armand, Kavous Solhjoo, Manoochehr Shabani Kordshooli, Mohammad Hasan Davami, Morteza Pourahmad, Vahideh Orfaee
Veterinary World.2017; 10(4): 386. CrossRef
Seroprevalence and genetic characterization of Toxoplasma gondii in masked palm civet ( Paguma larvata ) in Hainan province, tropical China
Guan-Yu Hou, Jun-Ming Zhao, Han-Lin Zhou, Guang Rong
Acta Tropica.2016; 162: 103. CrossRef