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Original Article

Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries
Hyeong-Kyu Jeon, Keeseon S. Eom
Korean J Parasitol 2019;57(5):481-487.
Published online October 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.5.481
Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.

Citations

Citations to this article as recorded by  Crossref logo
  • Deciphering the global genetic structure of Spirometra mansoni and S. erinaceieuropaei based on 28S ribosomal RNA: Insights into taxonomical revaluation and population dynamics
    Anil Kumar Nehra, Rasmita Panda, Prem Sagar Maurya, Ansu Kumari, Aman Dev Moudgil
    Parasitology Research.2025;[Epub]     CrossRef
  • Molecular Characterization of Spirometra erinaceieuropaei from Jungle Cat (Felis chaus) in North of Iran
    Mahboobeh Salimi, Meysam Sharifdini, Eshrat Beigom Kia
    Acta Parasitologica.2024; 69(1): 574.     CrossRef
  • Morphological observation and molecular phylogeny of Spirometra decipiens complex 1 (Cestoda: Diphyllobothriidae) found in cat from Chile
    Fernando Fredes, Rubén Mercado, Ismael Pereira Salas, Hiromu Sugiyama, Hirotaka Kobayashi, Hiroshi Yamasaki
    Parasitology International.2022; 87: 102493.     CrossRef
  • Insights to helminth infections in food and companion animals in Bangladesh: Occurrence and risk profiling
    Tilak Chandra Nath, Keeseon S. Eom, Seongjun Choe, Saiful Islam, Siblee Sadik Sabuj, Eva Saha, Rumman Hossain Tuhin, Barakaeli Abdieli Ndosi, Yeseul Kang, Sunmin Kim, Mohammed Mebarek Bia, Hansol Park, Dongmin Lee
    Parasite Epidemiology and Control.2022; 17: e00245.     CrossRef
  • Genetic structure of Spirometra mansoni (Cestoda: Diphyllobothriidae) populations in China revealed by a Target SSR-seq method
    Fang Fang Xu, Wen Qing Chen, Wei Liu, Sha Sha Liu, Yi Xing Wang, Jing Chen, Jing Cui, Xi Zhang
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Spirometra species from Asia: Genetic diversity and taxonomic challenges
    Hiroshi Yamasaki, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Porntip Laummaunwai, Mesa Un, Tongjit Thanchomnang, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M. Intapan, Wanchai Maleewong
    Parasitology International.2021; 80: 102181.     CrossRef
  • First Clinical Cases of Spirometrosis in Two Cats in Korea
    Joohyung Kim, Younsung Ock, Kihwan Yang, Seongjun Choe, Kyung-Mee Park, Wan-Kyu Lee, Kyung-Chul Choi, Soochong Kim, Dongmi Kwak, Seung-Hun Lee
    The Korean Journal of Parasitology.2021; 59(2): 153.     CrossRef
  • Low prevalence of spargana infection in farmed frogs in the Yangtze River Delta of China
    Xiaoli Zhang, Rongsheng Mi, Yehua Zhang, Shijie Zhang, Tao Sun, Haiyan Jia, Yan Huang, Haiyan Gong, Xiangan Han, Zhaoguo Chen
    Infection, Genetics and Evolution.2020; 85: 104466.     CrossRef
  • First identification and molecular phylogeny of Sparganum proliferum from endangered felid (Panthera onca) and other wild definitive hosts in one of the regions with highest worldwide biodiversity
    Juan Pablo Arrabal, Matías Gastón Pérez, Lucas Federico Arce, Laura Kamenetzky
    International Journal for Parasitology: Parasites and Wildlife.2020; 13: 142.     CrossRef
  • Ocular Sparganosis: The First Report of Spirometra ranarum in Thailand
    Wilai Saksirisampant, Chatanun Eamudomkarn, Hyeong-Kyu Jeon, Keeseon S. Eom, Buravej Assavapongpaiboon, Sunisa Sintuwong, Wasee Tulvatana
    The Korean Journal of Parasitology.2020; 58(5): 577.     CrossRef
  • 7,370 View
  • 121 Download
  • 10 Web of Science
  • Crossref

Brief Communication

RNA-sequencing Profiles of Cell Cycle?Related Genes Upregulated during the G2-Phase in Giardia lamblia
Juri Kim, Mee Young Shin, Soon-Jung Park
Korean J Parasitol 2019;57(2):185-189.
Published online April 30, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.2.185
To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

Citations

Citations to this article as recorded by  Crossref logo
  • Identification and confirmation of SUMOylation-modified proteins in Giardia trophozoites
    Hye Rim Yeo, Mee Young Shin, Juri Kim, Soon-Jung Park
    Parasites, Hosts and Diseases.2025; 63(3): 264.     CrossRef
  • Functional Differentiation of Cyclins and Cyclin-Dependent Kinases in Giardia lamblia
    Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park, Björn F. C. Kafsack
    Microbiology Spectrum.2023;[Epub]     CrossRef
  • In vitro erythrocyte production using human-induced pluripotent stem cells: determining the best hematopoietic stem cell sources
    Youn Keong Cho, Hyun-Kyung Kim, Soon Sung Kwon, Su-Hee Jeon, June-Won Cheong, Ki Taek Nam, Han-Soo Kim, Sinyoung Kim, Hyun Ok Kim
    Stem Cell Research & Therapy.2023;[Epub]     CrossRef
  • Spliceosomal introns in the diplomonad parasite Giardia duodenalis revisited
    Matthew H. Seabolt, Dawn M. Roellig, Konstantinos T. Konstantinidis
    Microbial Genomics .2023;[Epub]     CrossRef
  • A cell-cycle–dependent GARP-like transcriptional repressor regulates the initiation of differentiation in Giardia lamblia
    Han-Wei Shih, Germain C. M. Alas, Alexander R. Paredez
    Proceedings of the National Academy of Sciences.2022;[Epub]     CrossRef
  • Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
    Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park
    The Korean Journal of Parasitology.2022; 60(3): 163.     CrossRef
  • Hidden Diversity within Common Protozoan Parasites as Revealed by a Novel Genomotyping Scheme
    Matthew H. Seabolt, Konstantinos T. Konstantinidis, Dawn M. Roellig, Johanna Björkroth
    Applied and Environmental Microbiology.2021;[Epub]     CrossRef
  • A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lamblia
    Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park
    Parasites & Vectors.2021;[Epub]     CrossRef
  • An update on cell division of Giardia duodenalis trophozoites
    Francisco Alejandro Lagunas-Rangel, Janet Yee, Rosa María Bermúdez-Cruz
    Microbiological Research.2021; 250: 126807.     CrossRef
  • Nicotinamide induces G2 cell cycle arrest in Giardia duodenalis trophozoites and promotes changes in sirtuins transcriptional expression
    Francisco Alejandro Lagunas-Rangel, María Luisa Bazán-Tejeda, Enrique García-Villa, Rosa María Bermúdez-Cruz
    Experimental Parasitology.2020; 209: 107822.     CrossRef
  • 7,394 View
  • 127 Download
  • 10 Web of Science
  • Crossref

Original Article

Genetic Diversity of Ascaris in China Assessed Using Simple Sequence Repeat Markers
Chunhua Zhou, Shaoqing Jian, Weidong Peng, Min Li
Korean J Parasitol 2018;56(2):175-181.
Published online April 30, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.2.175
The giant roundworm Ascaris infects pigs and people worldwide and causes serious diseases. The taxonomic relationship between Ascaris suum and Ascaris lumbricoides is still unclear. The purpose of the present study was to investigate the genetic diversity and population genetic structure of 258 Ascaris specimens from humans and pigs from 6 sympatric regions in Ascaris-endemic regions of China using existing simple sequence repeat data. The microsatellite markers showed a high level of allelic richness and genetic diversity in the samples. Each of the populations demonstrated excess homozygosity (Ho<He, Fis>0). According to a genetic differentiation index (Fst=0.0593), there was a highlevel of gene flow in the Ascaris populations. A hierarchical analysis on molecular variance revealed remarkably high levels of variation within the populations. Moreover, a population structure analysis indicated that Ascaris populations fell into 3 main genetic clusters, interpreted as A. suum, A. lumbricoides, and a hybrid of the species. We speculated that humans can be infected with A. lumbricoides, A. suum, and the hybrid, but pigs were mainly infected with A. suum. This study provided new information on the genetic diversity and population structure of Ascaris from human and pigs in China, which can be used for designing Ascaris control strategies. It can also be beneficial to understand the introgression of host affiliation.

Citations

Citations to this article as recorded by  Crossref logo
  • Development and cross-amplification of novel SSR markers for population genetic analysis of Kitti’s hog-nosed bat (Craseonycteris thonglongyai) in Thailand
    Supajit Sraphet, Nattaya Srisawad, Nawarat Suksee, Piengtawan Tappiban, Surachit Waengsothorn, Kridsada Chaichoun, Ruangrat Buddhirongawatr, Sarin Suwanpakdee, Siriporn Tungsudjai, Poonyapat Sedwisai, Tatiyanuch Chamsai, Thekhawet Weluwanarak, Nareerat Sa
    Scientific Reports.2025;[Epub]     CrossRef
  • Worldwide absence of canonical benzimidazole resistance-associated mutations within β-tubulin genes from Ascaris
    Ben P. Jones, Kezia Kozel, Allen Jethro I. Alonte, Kennesa Klariz R. Llanes, Alexandra Juhász, Umer Chaudhry, Sara Roose, Peter Geldhof, Vicente Y. Belizario, Peter Nejsum, J. Russell Stothard, E. James LaCourse, Arnoud H. M. van Vliet, Vachel Gay V. Pall
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Ascaris suum – A zoonosis in Bali, Indonesia
    Kadek Karang Agustina, I Made Ady Wirawan, I Made Sudarmaja, I Made Subrata, Nyoman Sadra Dharmawan
    Tropical Parasitology.2023; 13(2): 100.     CrossRef
  • Molecular epidemiology ofAscarisspecies recovered from humans and pigs in Cameroon
    Vanessa R Nkouayep, Donald P McManus, Mpoame Mbida, Catherine A Gordon, Peter Nejsum
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2022; 116(10): 949.     CrossRef
  • Development of allele-specific PCR methodology (AS-PCR) to screening A. lumbricoides and A. suum
    Talita Rodrigues dos Santos, Luis Fernando Viana Furtado, Andreina de Carvalho Araujo, Celi da Silva Medeiros, Pedro Henrique Vieira Germano, Valéria Nayara Gomes Mendes de Oliveira, Elida Mara Leite Rabelo
    Parasitology Research.2022; 121(8): 2389.     CrossRef
  • Genotyping of Ascaris spp. infecting humans and pigs in Italy, Slovakia and Colombia
    Serena Cavallero, Silvia Rondón, Ivan Acevedo Monterrosa, Viliam Šnábel, Ingrid Papajová, Mária Goldová, Gabriela Štrkolcová, Luis Caraballo, Nathalie Acevedo, Stefano D'Amelio
    Infection, Genetics and Evolution.2021; 94: 104997.     CrossRef
  • Mitochondrial phylogenomics of human-type Ascaris, pig-type Ascaris, and hybrid Ascaris populations
    Chunhua Zhou, Ting Guo, Yuanyu Deng, Jingjing He, Shan Ouyang, Xiaoping Wu
    Veterinary Parasitology.2020; 287: 109256.     CrossRef
  • 8,443 View
  • 128 Download
  • 5 Web of Science
  • Crossref

Brief Communications

Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene
Hong-Rui Ning, Si-Yang Huang, Jin-Lei Wang, Qian-Ming Xu, Xing-Quan Zhu
Korean J Parasitol 2015;53(3):345-348.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.345
Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

Citations

Citations to this article as recorded by  Crossref logo
  • Effects of latent infection of Toxoplasma gondii strains with different genotypes on mouse behavior and brain transcripts
    Bei-Bei Zhou, Hong-Jie Dong, Hang Sun, Xiao-Man Xie, Huan-Huan Xie, Wen-Ju Zhu, Ya-Nan Li, Chao Xu, Jian-Ping Cao, Gui-Hua Zhao, Kun Yin
    Parasites & Vectors.2025;[Epub]     CrossRef
  • First Report on the Molecular Detection and Genetic Characterization of Toxoplasma gondii From Donkeys in Kenya
    Fredrick O. Obonyo, Ndichu Maingi, Samuel M. Githigia, Kevin O. Ochwedo, Anne A. Owiti, Evans N. Nyaboga
    Acta Parasitologica.2024; 69(3): 1480.     CrossRef
  • 9,535 View
  • 101 Download
  • 2 Web of Science
  • Crossref
Sequence Diversity in MIC6 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations
Zhong-Yuan Li, Hui-Qun Song, Jia Chen, Xing-Quan Zhu
Korean J Parasitol 2015;53(3):341-344.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.341
Toxoplasma gondii is an opportunistic protozoan parasite that can infect almost all warm-blooded animals including humans with a worldwide distribution. Micronemes play an important role in invasion process of T. gondii, associated with the attachment, motility, and host cell recognition. In this research, sequence diversity in microneme protein 6 (MIC6) gene among 16 T. gondii isolates from different hosts and geographical regions and 1 reference strain was examined. The results showed that the sequence of all the examined T. gondii strains was 1,050 bp in length, and their A + T content was between 45.7% and 46.1%. Sequence analysis presented 33 nucleotide mutation positions (0-1.1%), resulting in 23 amino acid substitutions (0-2.3%) aligned with T. gondii RH strain. Moreover, T. gondii strains representing the 3 classical genotypes (Type I, II, and III) were separated into different clusters based on the locus of MIC6 using phylogenetic analyses by Bayesian inference (BI), maximum parsimony (MP), and maximum likelihood (ML), but T. gondii strains belonging to ToxoDB #9 were separated into different clusters. Our results suggested that MIC6 gene is not a suitable marker for T. gondii population genetic studies.
  • 10,062 View
  • 93 Download
  • 1 Web of Science

Original Article

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Shuai Wang, Aiping Cao, Xun Li, Qunli Zhao, Yuan Liu, Hua Cong, Shenyi He, Huaiyu Zhou
Korean J Parasitol 2015;53(3):253-258.
Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.253
Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

Citations

Citations to this article as recorded by  Crossref logo
  • Superoxide Dismutases in Immune Regulation and Infectious Diseases
    Tong Liu, Jiajin Shang, Qijun Chen
    Antioxidants.2025; 14(7): 809.     CrossRef
  • Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
    Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
    BMC Infectious Diseases.2017;[Epub]     CrossRef
  • 10,458 View
  • 102 Download
  • 2 Web of Science
  • Crossref

Brief Communications

Genetic Polymorphisms in VIR Genes among Indian Plasmodium vivax Populations
Purva Gupta, Veena Pande, Aparup Das, Vineeta Singh
Korean J Parasitol 2014;52(5):557-564.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.557

The vir genes are antigenic genes and are considered to be possible vaccine targets. Since India is highly endemic to Plasmodium vivax, we sequenced 5 different vir genes and investigated DNA sequence variations in 93 single-clonal P. vivax isolates. High variability was observed in all the 5 vir genes; the vir 1/9 gene was highly diverged across Indian populations. The patterns of genetic diversity do not follow geographical locations, as geographically distant populations were found to be genetically similar. The results in general present complex genetic diversity patterns in India, requiring further in-depth population genetic and functional studies.

Citations

Citations to this article as recorded by  Crossref logo
  • Genomic dynamics of clinical Plasmodium vivax: comparative genomic hybridization in severe malaria cases
    Sampreeti Tahbildar, Pon Arunachalam Boopathi, Sanjay Kumar Kochar, Dhanpat Kumar Kochar, Mohamed Aiyaz, Raja C. Mugasimangalam, Sudha N. Rao, Ashis Das
    Frontiers in Malaria.2025;[Epub]     CrossRef
  • Population genetic analysis of Plasmodium vivax vir genes in Pakistan
    Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo
    Parasites, Hosts and Diseases.2024; 62(3): 313.     CrossRef
  • Misidentification of Plasmodium Species by Cross-Reacting Primers and Cerebral Malaria Caused by Plasmodium vivax
    Anoopkrishna Rai, Deepak Sebastian Pinto, Praveen Rai, Srinivas Teerthanath, Indrani Karunasagar, Rama Adiga
    Journal of Health and Allied Sciences NU.2023; 13(04): 563.     CrossRef
  • Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
    Youn-Kyoung Goo
    Microorganisms.2022; 10(6): 1083.     CrossRef
  • Humoral and cellular immune response to Plasmodium vivax VIR recombinant and synthetic antigens in individuals naturally exposed to P. vivax in the Republic of Korea
    Sanghyun Lee, Young-Ki Choi, Youn-Kyoung Goo
    Malaria Journal.2021;[Epub]     CrossRef
  • Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation
    Ui-han Son, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Hae Soo Yun, Jung-Yeon Kim, So-Young Joo, Sookwan Jeong, Man Hee Rhee, Yeonchul Hong, Dong-Il Chung, Dongmi Kwak, Youn-Kyoung Goo
    The Korean Journal of Parasitology.2017; 55(2): 149.     CrossRef
  • Clinical manifestations and molecular mechanisms in the changing paradigm of vivax malaria in India
    Purva Gupta, Rajni Sharma, Jagdish Chandra, Virender Kumar, Ruchi Singh, Veena Pande, Vineeta Singh
    Infection, Genetics and Evolution.2016; 39: 317.     CrossRef
  • Acute kidney injury in malaria: An update
    Anand Chellappan, D.S. Bhadauria
    Clinical Queries: Nephrology.2016; 5(1): 26.     CrossRef
  • Plasmodium vivax msp-3α polymorphisms: analysis in the Indian subcontinent
    Anju Verma, Hema Joshi, Vineeta Singh, Anup Anvikar, Neena Valecha
    Malaria Journal.2016;[Epub]     CrossRef
  • Design, construction and validation of a Plasmodium vivax microarray for the transcriptome profiling of clinical isolates
    Pon Arunachalam Boopathi, Amit Kumar Subudhi, Sheetal Middha, Jyoti Acharya, Raja Chinnadurai Mugasimangalam, Sanjay Kumar Kochar, Dhanpat Kumar Kochar, Ashis Das
    Acta Tropica.2016; 164: 438.     CrossRef
  • 10,786 View
  • 102 Download
  • 9 Web of Science
  • Crossref
Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence
Guochao Zheng, Muhamd Alsarakibi, Yuanjia Liu, Wei Hu, Qin Luo, Liping Tan, Guoqing Li
Korean J Parasitol 2014;52(3):299-304.
Published online June 26, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.3.299

This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.

Citations

Citations to this article as recorded by  Crossref logo
  • Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals
    Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan
    PLOS Neglected Tropical Diseases.2025; 19(4): e0013021.     CrossRef
  • Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in humans and dogs from Fujian Province, Southeast China
    Si-Ang Li, Yu-Ling Lin, Yun-Peng Bai, Fuli Wen, Li-Yuan Huang, Wen-Yuan Miao, Dong-Hui Zhou
    Food and Waterborne Parasitology.2025; 40: e00278.     CrossRef
  • Molecular Detection of Giardia Duodenalis Sub-Assemblages among Human Diarrheal Patients in Northwestern Iran
    Firoozeh Naseri, Shahram Khademvatan, Khosrow Hazrati Tappeh, Sama Mahmoodzadeh, Elena A. Silivanova, Shamal Abdullah Al-Muffti, Saber Gholizadeh
    Health Science Monitor.2025; 4(3): 212.     CrossRef
  • Cryptosporidium and Giardia in cats and dogs: What is the real zoonotic risk?
    Amanda D. Barbosa, Siobhon Egan, Yaoyu Feng, Lihua Xiao, Una Ryan
    Current Research in Parasitology & Vector-Borne Diseases.2023; 4: 100158.     CrossRef
  • Dogs as a source for the spreading of enteric parasites including zoonotic ones in Giza Province, Egypt
    Marwa M. Khalifa, Ehab A. Fouad, Nancy O. Kamel, Hend M. Auda, Mohamed M. El-Bahy, Reem M. Ramadan
    Research in Veterinary Science.2023; 161: 122.     CrossRef
  • Epizootiology and biological characteristics of echinococcosis in agricultural animals, dogs, wild carnivores, and rodents in the Western region of the Republic of Kazakhstan
    Abirova Ilana, Baitlesov Erbulat Upievich, Kereyev Abzal Kenesovich, Mamanova Saltanat Bekbosynovna, Zakirova Faruza Bakitzhanovna, Murzabaev Kenzhebek Esmagambetovich, Sengaliyev Yerbol Maratovich, Satybaev Berik Garipullievich, Abdrakhmanov Rinat Gabdul
    Veterinary World.2023; : 2277.     CrossRef
  • Global prevalence of Giardia duodenalis in cattle: A systematic review and meta-analysis
    Ali Taghipour, Mitra Sharbatkhori, Farideh Tohidi, Mohammad R. Ghanbari, Panagiotis Karanis, Meysam Olfatifar, Hamidreza Majidiani, Sasan Khazaei, Saeed Bahadory, Ehsan Javanmard
    Preventive Veterinary Medicine.2022; 203: 105632.     CrossRef
  • Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in pet dogs in Xinjiang, China
    Yangwenna Cao, Chen Fang, Jinhua Deng, Fuchang Yu, Dingyun Ma, Liwen Chuai, Tian Wang, Meng Qi, Junqiang Li
    Parasitology Research.2022; 121(5): 1429.     CrossRef
  • Infection rate and genetic diversity of Giardia duodenalis assemblage C in Iranian stray dogs, targeting the glutamate dehydrogenase gene
    Asghar Fazaeli, Mohammad Hasan Kohansal, Adel Spotin, Ali Haniloo, Abbasali Nourian, Alireza Khiabani, Abolghasem Siyadatpanah, Roghayeh Norouzi, Veeranoot Nissapatorn
    Veterinary World.2021; 14(2): 419.     CrossRef
  • Development of HRM real-time PCR for assemblage characterization of Giardia lamblia
    Zahra Bahramdoost, Hamed Mirjalali, Parvin Yavari, Ali Haghighi
    Acta Tropica.2021; 224: 106109.     CrossRef
  • Zoonotic giardiasis: an update
    Weilong Cai, Una Ryan, Lihua Xiao, Yaoyu Feng
    Parasitology Research.2021; 120(12): 4199.     CrossRef
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Sequence Analysis of cytb Gene in Echinococcus granulosus from Western China
Xiuqin Zhong, Ning Wang, Dandan Hu, Jiahai Wang, Tianyu Liu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
Korean J Parasitol 2014;52(2):205-209.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.205

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance in China. Our main
objective
was to discuss the genotypes and genetic diversity of E. granulosus present in domestic animals and humans in western China. A total of 45 hydatid cyst samples were collected from sheep, humans, and a yak and subjected to an analysis of the sequences of mitochondrial cytochrome b (cytb) gene. The amplified PCR product for all samples was a 1,068 bp band. The phylogenetic analysis showed that all 45 samples were identified as E. granulosus (genotype G1). Ten haplotypes were detected among the samples, with the main haplotype being H1. The haplotype diversity was 0.626, while the nucleotide diversity was 0.001. These results suggested that genetic diversity was low among our samples collected from the west of China based on cytb gene analysis. These findings may provide more information on molecular characteristics of E. granulosus from this Chinese region.

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Case Reports

Four Cases of Taenia saginata Infection with an Analysis of COX1 Gene
Jaeeun Cho, Bong-Kwang Jung, Hyemi Lim, Min-Jae Kim, Thanapon Yooyen, Dongmin Lee, Keeseon S. Eom, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2014;52(1):79-83.
Published online February 19, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.1.79

Human taeniases had been not uncommon in the Republic of Korea (=Korea) until the 1980s. The prevalence decreased and a national survey in 2004 revealed no Taenia egg positive cases. However, a subsequent national survey in 2012 showed 0.04% (10 cases) prevalence of Taenia spp. eggs suggesting its resurgence in Korea. We recently encountered 4 cases of Taenia saginata infection who had symptoms of taeniasis that included discharge of proglottids. We obtained several proglottids from each case. Because the morphological features of T. saginata are almost indistinguishable from those of Taenia asiatica, molecular analyses using the PCR-RFLP and DNA sequencing of the cytochrome c oxidase subunit 1 (cox1) were performed to identify the species. The PCR-RFLP patterns of all of the 4 specimens were consistent with T. saginata, and the cox1 gene sequence showed 99.8-100% identity with that of T. saginata reported previously from Korea, Japan, China, and Cambodia. All of the 4 patients had the history of travel abroad but its relation with contracting taeniasis was unclear. Our findings may suggest resurgence of T. saginata infection among people in Korea.

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A Case of Diphyllobothrium nihonkaiense Infection as Confirmed by Mitochondrial COX1 Gene Sequence Analysis
Sang Hyun Park, Keeseon S. Eom, Min Sun Park, Oh Kyoung Kwon, Hyo Sun Kim, Jai Hoon Yoon
Korean J Parasitol 2013;51(4):471-473.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.471

Diphyllobothrium nihonkaiense has been reported in Korea as Diphyllobothrium latum because of their close morphologic resemblance. We have identified a human case of D. nihonkaiense infection using the mitochondrial cytochrome c oxidase subunit I (cox1) gene sequence analysis. On 18 February 2012, a patient who had consumed raw fish a month earlier visited our outpatient clinic with a long tapeworm parasite excreted in the feces. The body of the segmented worm was 2 m long and divided into the scolex (head) and proglottids. It was morphologically close to D. nihonkaiense and D. latum. The cox1 gene analysis showed 99.4% (340/342 bp) homology with D. nihonkaiense but only 91.8% (314/342 bp) homology with D. latum. The present study suggested that the Diphyllobothrium spp. infection in Korea should be analyzed with specific DNA sequence for an accurate species identification.

Citations

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    Hong-Ja Kim, Keeseon S. Eom, Min Seo
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  • Two Human Cases of Diphyllobothrium nihonkaiense Infection in Korea
    Su-Min Song, Hye-Won Yang, Min Kyu Jung, Jun Heo, Chang Min Cho, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung
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    Sung-Chul Choi, Soo-Young Lee, Hyun-Ouk Song, Jae-Sook Ryu, Myoung-Hee Ahn
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  • 82 Download
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Original Articles

Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
Korean J Parasitol 2013;51(4):401-411.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.401

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by analyzing both the nuclear 18S small subunit ribosomal RNA (18S rRNA) and mitochondrial 16S rRNA gene loci, 27 clinical Acanthamoeba strains that caused AK in Japan were classified into 3 genotypes, T3 (3 strains), T4 (23 strains), and T5 (one strain). Most haplotypes were identical to the reference haplotypes reported from all over the world, and thus no specificity of the haplotype distribution in Japan was found. The T4 sub-genotype analysis using the 16S rRNA gene locus also revealed a clear sub-conformation within the T4 cluster, and lead to the recognition of a new sub-genotype T4i, in addition to the previously reported sub-genotypes T4a-T4h. Furthermore, 9 out of 23 strains in the T4 genotype were identified to a specific haplotype (AF479533), which seems to be a causal haplotype of AK. While heterozygous nuclear haplotypes were observed from 2 strains, the mitochondrial haplotypes were homozygous as T4 genotype in the both strains, and suggested a possibility of nuclear hybridization (mating reproduction) between different strains in Acanthamoeba. The nuclear 18S rRNA gene and mitochondrial 16S rRNA gene loci of Acanthamoeba spp. possess different unique characteristics usable for the genotyping analyses, and those specific features could contribute to the establishment of molecular taxonomy for the species complex of Acanthamoeba.

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    Daniele Corsaro
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    Derya Dirim Erdogan, Mehmet Aykur, Nur Selvi Gunel, Melis Palamar, Ozlem Barut Selver, Buket Ozel, Ayse Yagci, Cumhur Gunduz, Hande Dagci
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    Daniele Corsaro
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    Maria Luisa Nunes Diehl, Júlia Paes, Marilise Brittes Rott
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    Omid Ahmadi, Yousef Sharifi, Nazgol Khosravinia, Elham Moghaddas, Mohammad Akhoundi, Reza Fotouhi-Ardakani, Jaber Asadi, Amir Hossein Mohamadzade, Ghodratolah Salehi Sangani, Hamed Mirjalali, Mehdi Zarean
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    Daniele Corsaro
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    Elizabeth Jemaiyo Matey, Masaharu Tokoro, Takehiro Nagamoto, Tetsushi Mizuno, Matilda Chelimo Saina, Xiuqiong Bi, Jane A. Oyombra, Paul Okumu, Benard Kibet Langat, Willie Kipkemboi Sang, Elijah Maritim Songok, Hiroshi Ichimura
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    Elizabeth J. Matey, Masaharu Tokoro, Tetsushi Mizuno, Takahiro Matsumura, Takehiro Nagamoto, Xiuqiong Bi, Jane A. Oyombra, Willie K. Sang, Elijah M. Songok, Hiroshi Ichimura
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    Azar Shokri, Shahabeddin Sarvi, Ahmad Daryani, Mehdi Sharif
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Expressed Sequence Tag Analysis of the Erythrocytic Stage of Plasmodium berghei
Ji-Woong Seok, Yong-Seok Lee, Eun-Kyung Moon, Jung-Yub Lee, Bijay Kumar Jha, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong
Korean J Parasitol 2011;49(3):221-228.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.221

Rodent malaria parasites, such as Plasmodium berghei, are practical and useful model organisms for human malaria research because of their analogies to the human malaria in terms of structure, physiology, and life cycle. Exploiting the available genetic sequence information, we constructed a cDNA library from the erythrocytic stages of P. berghei and analyzed the expressed sequence tag (EST). A total of 10,040 ESTs were generated and assembled into 2,462 clusters. These EST clusters were compared against public protein databases and 48 putative new transcripts, most of which were hypothetical proteins with unknown function, were identified. Genes encoding ribosomal or membrane proteins and purine nucleotide phosphorylases were highly abundant clusters in P. berghei. Protein domain analyses and the Gene Ontology functional categorization revealed translation/protein folding, metabolism, protein degradation, and multiple family of variant antigens to be mainly prevalent. The presently-collected ESTs and its bioinformatic analysis will be useful resources to identify for drug target and vaccine candidates and validate gene predictions of P. berghei.

  • 9,236 View
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Genetic Variability of Antigen B among Echinococcus granulosus Egyptian Isolates
Gihan M. Tawfeek, Hala S. Elwakil, Nabil S. Awad, Laila El-Hoseiny, Hala S. Thabet, Rania M. Sarhan, Samar K. Darweesh, Wagida A. Anwar
Korean J Parasitol 2009;47(3):259-264.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.259

Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.

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  • Characterization of antigen B subunit 2 (AgB2) gene polymorphism in sheep isolates of Echinococcus granulosus sensu lato and effect on serologic response
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    Harun Kaya Kesik, Sami Simsek, Seyma Gunyakti Kilinc, Ergun Koroglu
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    Mohey Abdelhafez Hassanain, Raafat Mohamed Shaapan, Fathia Abdelrazik M. Khalil
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    Yosra H. Alam-Eldin, Heba E. Abdel Aaty, Mona A. Ahmed
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    Abdul Jabbar, Robin B. Gasser
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    Gihan M. Tawfeek, Hala S. Elwakil, Laila El-hoseiny, Hala S. Thabet, Rania M. Sarhan, Nabil S. Awad, Wagida A. Anwar
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Interaction between Parasitophorous Vacuolar Membrane-associated GRA3 and Calcium Modulating Ligand of Host Cell Endoplasmic Reticulum in the Parasitism of Toxoplasma gondii
Ji Yeon Kim, Hye-Jin Ahn, Kyung Ju Ryu, Ho-Woo Nam
Korean J Parasitol 2008;46(4):209-216.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.209

A monoclonal antibody against Toxoplasma gondii of Tg556 clone (Tg556) blotted a 29 kDa protein, which was localized in the dense granules of tachyzoites and secreted into the parasitophorous vacuolar membrane (PVM) after infection to host cells. A cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg556, and the full-length was completed by 5'-RACE of 2,086 bp containing an open reading frame (ORF) of 669 bp. The ORF encoded a polypeptide of 222 amino acids homologous to the revised GRA3 but not to the first reported one. The polypeptide has 3 hydrophobic moieties of an N-terminal stop transfer sequence and 2 transmembrane domains (TMD) in posterior half of the sequence, a cytoplasmic localization motif after the second TMD and an endoplasmic reticulum (ER) retrival motif in the C-terminal end, which suggests GRA3 as a type III transmembrane protein. With the ORF of GRA3, yeast two-hybrid assay was performed in HeLa cDNA expression library, which resulted in the interaction of GRA3 with calcium modulating ligand (CAMLG), a type II transmembrane protein of ER. The specific binding of GRA3 and CAMLG was confirmed by glutathione S-transferase (GST) pull-down and immunoprecipitation assays. The localities of fluorescence transfectionally expressed from GRA3 and CAMLG plasmids were overlapped completely in HeLa cell cytoplasm. In immunofluorescence assay, GRA3 and CAMLG were shown to be co-localized in the PVM of host cells. Structural binding of PVM-inserted GRA3 to CAMLG of ER suggested the receptor-ligand of ER recruitment to PVM during the parasitism of T. gondii.

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Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae
Hae Kyung Park, Seong Won Chang, Se Won Kang, Min Kyoung Cho, Sun Hee Choi, Yeon Chul Hong, Yong Seok Lee, Hae Jin Jeong, Hak Sun Yu
Korean J Parasitol 2008;46(2):59-63.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.59

In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

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  • Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage
    Bin Tang, Mingyuan Liu, Libo Wang, Shenye Yu, Haining Shi, Pascal Boireau, Vasile Cozma, Xiuping Wu, Xiaolei Liu
    Parasites & Vectors.2015;[Epub]     CrossRef
  • Molecular characterization of 45kDa aspartic protease of Trichinella spiralis
    Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
    Veterinary Parasitology.2012; 190(3-4): 510.     CrossRef
  • Protease-Activated Receptor 2 Is Involved in Th2 Responses againstTrichinella spiralisInfection
    Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu
    The Korean Journal of Parasitology.2011; 49(3): 235.     CrossRef
  • Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
    Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
    Veterinary Parasitology.2009; 164(2-4): 242.     CrossRef
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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu
Korean J Parasitol 2006;44(4):313-320.
Published online December 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.4.313

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.

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    The Korean Journal of Parasitology.2022; 60(4): 229.     CrossRef
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    Jinfang Wu, Huatao Xie
    Journal of International Medical Research.2021;[Epub]     CrossRef
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    Kelvin Ho-Nam Wan
    World Journal of Ophthalmology.2014; 4(3): 63.     CrossRef
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    Yasin A. Khan, Renata T. Kashiwabuchi, Suy Anne Martins, Juan M. Castro-Combs, Sachin Kalyani, Philip Stanley, David Flikier, Ashley Behrens
    Ophthalmology.2011; 118(2): 324.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2010; 29(6): 680.     CrossRef
  • Twenty Years of Acanthamoeba Keratitis
    F R S Carvalho, A S Foronda, M J Mannis, A L Höfling-Lima, R Belfort, Denise de Freitas
    Cornea.2009; 28(5): 516.     CrossRef
  • Molecular Phylogeny of Acanthamoeba
    Hyun Hee Kong
    The Korean Journal of Parasitology.2009; 47(Suppl): S21.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2009; 28(3): 348.     CrossRef
  • Trends in Microbial Keratitis Associated With Orthokeratology
    Kathleen G. Watt, Helen A. Swarbrick
    Eye & Contact Lens: Science & Clinical Practice.2007; 33(6): 373.     CrossRef
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Brief Communication

Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Korean J Parasitol 2006;44(3):251-254.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.251

Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the mAb, a colony containing a 3'-UTR was probed in a T. gondii cDNA expression library. A full length cDNA sequence of the rhoptry protein was completed after 5'-RACE, which consisted of 1,908 bp with a 1,443 bp ORF. The deduced amino acid sequence of ROP6 consisted of a polypeptide of 480 amino acids without significant homology to any other known proteins. This sequence contains an amino terminal stop transfer sequence downstream of a short neutral sequence, hydrophilic middle sequence, and hydrophobic carboxy terminus. It is suggested that the ROP6 is inserted into the rhoptry membrane with both N- and C-termini.

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  • A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization
    Fatemeh Rezaei, Shahabeddin Sarvi, Mahdi Sharif, Seyed Hossein Hejazi, Abdol sattar Pagheh, Sargis A. Aghayan, Ahmad Daryani
    Microbial Pathogenesis.2019; 126: 172.     CrossRef
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    Yang Zhang, Bo Shiun Lai, Mario Juhas, Yun Zhang
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    Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani
    Journal of Microbiological Methods.2019; 165: 105696.     CrossRef
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    Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam
    Protein Expression and Purification.2014; 101: 146.     CrossRef
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    Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour
    Jundishapur Journal of Microbiology.2014;[Epub]     CrossRef
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    John C. Boothroyd, Jean-Francois Dubremetz
    Nature Reviews Microbiology.2008; 6(1): 79.     CrossRef
  • Characterization of a New Gene <italic>wx2</italic> in <italic>Toxoplasma gondii</italic>
    Xiang Wu, Qiong Zhang, Kui Tan, Ronghua Xie, Jiubo Fan, Hengping Shu, Shiping Wang
    Acta Biochimica et Biophysica Sinica.2007; 39(7): 475.     CrossRef
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Original Articles
Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea
Jae-Hwan Park, Sang-Mee Guk, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Jong-Yil Chai
Korean J Parasitol 2006;44(1):27-33.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.27

Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified Ziehl-Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases constituted 7 (41.2%) of 17 examined, and goat cases 3 (42.9%) of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.

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Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
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DOI: https://doi.org/10.3347/kjp.2005.43.1.7

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.

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Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii
Aifen Lin, Eun-Hee Shin, Tae-Yun Kim, Jae-Hwan Park, Sang-Mee Guk, Jong-Yil Chai
Korean J Parasitol 2005;43(1):27-32.
Published online March 20, 2005
DOI: https://doi.org/10.3347/kjp.2005.43.1.27

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.

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Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
Korean J Parasitol 2004;42(3):129-135.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.129

We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.

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    Wilhelm F Sirgel, Patricio Artigas, M Dolores Bargues, Santiago Mas-Coma
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Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients
Hye-Jin Ahn, Sera Kim, Ho-Woo Nam
Korean J Parasitol 2003;41(2):89-96.
Published online June 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.2.89

Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

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    M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay
    Protein Expression and Purification.2009; 65(1): 8.     CrossRef
  • Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
    Houshuang Zhang, Eung-goo Lee, Min Liao, Muller K.A. Compaore, Guohong Zhang, Osamu Kawase, Kozo Fujisaki, Chihiro Sugimoto, Yoshifumi Nishikawa, Xuenan Xuan
    Molecular and Biochemical Parasitology.2007; 153(2): 141.     CrossRef
  • Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii-specific IgG using Faradaic impedance spectroscopy, CV and QCM
    Yanjun Ding, Hua Wang, Guoli Shen, Ruqin Yu
    Analytical and Bioanalytical Chemistry.2005; 382(7): 1491.     CrossRef
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    Infection and Immunity.2004; 72(9): 5515.     CrossRef
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Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi
Hyun-Hee Kong, Mee-Yeul Hwang, Hyo-Kyung Kim, Dong-Il Chung
Korean J Parasitol 2001;39(2):151-160.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.151

Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.

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    Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
    The Korean Journal of Parasitology.2009; 47(2): 103.     CrossRef
  • Acanthamoeba Interaction with Extracellular Matrix Glycoproteins: Biological and Biochemical Characterization and Role in Cytotoxicity and Invasiveness
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  • Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba
    Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
    The Korean Journal of Parasitology.2006; 44(4): 331.     CrossRef
  • Acanthamoeba healyi: Molecular cloning and characterization of a coronin homologue, an actin-related protein
    Eleonor T. Baldo, Eun-Kyung Moon, Hyun-Hee Kong, Dong-Il Chung
    Experimental Parasitology.2005; 110(2): 114.     CrossRef
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Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains
Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2000;38(3):151-158.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.151

Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA.

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Immunodiagnosis of clonorchiasis using a recombinant antigen
Tai-Soon Yong, Hye-Jin Yang, Soon-Jung Park, Yu-Kyoung Kim, Du-Ho Lee, Sang-Mi Lee
Korean J Parasitol 1998;36(3):183-190.
Published online September 30, 1998
DOI: https://doi.org/10.3347/kjp.1998.36.3.183

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a β-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

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