Parasites, Hosts and Diseases

"cyst"

Brief Communication

Partial characterization of a cysteine protease inhibitor of Plasmodium vivax: Tuấn Cường Võ, Jung-Mi Kang, Hương Giang Lê, Byoung-Kuk Na; Parasites Hosts Dis 2025;63(4):354-359.
Published online November 19, 2025; DOI: https://doi.org/10.3347/PHD.25043

Abstract
PDF

Cysteine proteases play key roles in the biology of Plasmodium parasites and are recognized as antimalarial drug targets. Because these enzymes are involved in diverse biological functions, precise regulation is required to prevent unnecessary damage to both parasites and hosts. In this study, we identified an endogenous inhibitor of cysteine protease of Plasmodium vivax (PvICP) and characterized its biochemical properties. PvICP was found to share highly similar structural characteristics with orthologous proteins from other Plasmodium species. Recombinant PvICP (rPvICP) expressed in Escherichia coli showed a broad range of inhibitory activity against falcipain family cysteine proteases, including vivapain-3, vivapain-4, falcipain-3, malapain-2, and malapain-4, with more potent inhibitory activity against vivapain-3 and vivapain-4. rPvICP’s inhibitory activity was not significantly affected by pH, suggesting its broad biological functions. These findings provide new insights into PvICP and lay the groundwork for future studies exploring its biological significance and potential as a therapeutic target in malaria research.

272 View
16 Download

Original Articles

Identification of essential genes for Acanthamoeba castellanii excystation during encystation and excystation: Min-Jeong Kim, Hye-Jeong Jo, Fu-Shi Quan, Ki Back Chu, Hyun-Hee Kong, Eun-Kyung Moon; Parasites Hosts Dis 2024;62(4):399-407.
Published online November 22, 2024; DOI: https://doi.org/10.3347/PHD.24062

Acanthamoeba is an opportunistic pathogen that causes Acanthamoeba keratitis, granulomatous amoebic encephalitis, and other cutaneous diseases. The life cycle of Acanthamoeba consists of 2 stages of trophozoites and cysts. Under adverse environmental conditions, Acanthamoeba encysts, while the conditions become favorable for growth, it reverts to the trophozoite form. Acanthamoeba excystation is crucial for its proliferation and can lead to recurrent infections after incomplete treatment. To identify the factors involved in excystation, A. castellanii was subjected to either encystation- or excystation-inducing conditions, and gene expression profiles were compared using mRNA sequencing. A. castellanii samples were collected at 8 h intervals for analysis under both conditions. Differentially expressed gene analysis revealed that 1,214 and 1,163 genes were upregulated and downregulated, respectively, by more than 2-fold during early excystation. Five genes markedly upregulated in early excystation (ACA1_031140, ACA1_032330, ACA1_374400, ACA1_275740, and ACA1_112650) were selected, and their expression levels were confirmed via real-time PCR. Small interfering RNA (siRNA) targeting these 5 genes was transfected into Acanthamoeba and gene knockdown was validated through real-time PCR. The silencing of ACA1_031140, ACA1_032330, ACA1_374400, and ACA1_112650 inhibited excystation and suggested that these genes might be essential for excystation. Our findings provide valuable insights for suppressing Acanthamoeba proliferation and recurrence.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Integrating molecular pathogenesis and predictive modelling in granulomatous amoebic encephalitis due to Acanthamoeba : a digital twin framework for personalised therapy
Ruqaiyyah Siddiqui, David Lloyd, Naveed Ahmed Khan
Infectious Diseases.2025; : 1. CrossRef
Microarray-based characterization of airway inflammation induced by environmental Acanthamoeba exposure versus the ovalbumin-alum model
Shin Ae Kang, Hak Sun Yu
Parasites, Hosts and Diseases.2025; 63(4): 327. CrossRef

2,352 View
137 Download
2 Web of Science
Crossref

CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products: Young Ah Lee, Myeong Heon Shin; Parasites Hosts Dis 2024;62(3):270-280.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24046

Abstract
PDF

Trichomoniasis is caused by a sexually transmitted flagellate protozoan parasite Trichomonas vaginalis. T. vaginalis-derived secretory products (TvSP) contain lipid mediators such as leukotriene B₄ (LTB₄) and various cysteinyl leukotrienes (CysLTs) which included LTC₄, LTD₄, and LTE₄. However, the signaling mechanisms by which T. vaginalis-induced CysLTs stimulate interleukin (IL)-8 production in human mast cells remain unclear. In this study, we investigated these mechanisms in human mast cells (HMC-1). Stimulation with TvSP resulted in increased intracellular reactive oxygen species (ROS) generation and NADPH oxidase 2 (NOX2) activation compared to unstimulated cells. Pre-treatment with NOX2 inhibitors such as diphenyleneiodonium chloride (DPI) or apocynin significantly reduced ROS production in TvSP-stimulated HMC-1 cells. Additionally, TvSP stimulation increased NOX2 protein expression and the translocation of p47^phox from the cytosol to the membrane. Pretreatment of HMC-1 cells with PI3K or PKC inhibitors reduced TvSP-induced p47^phox translocation and ROS generation. Furthermore, NOX2 inhibitors or NOX2 siRNA prevented CREB phosphorylation and IL-8 gene expression or protein secretion induced by TvSP. Pretreatment with a CysLTR antagonist significantly inhibited TvSP-induced ROS production, CREB phosphorylation, and IL-8 production. These results indicate that CysLT-mediated activation of NOX2 plays a crucial role in ROS-dependent IL-8 production in human mast cells stimulated by T. vaginalis-secreted CysLTs. These findings enhance our understanding of the inflammatory response in trichomoniasis and may inform the development of targeted therapies to mitigate this response.

Citations

Citations to this article as recorded by

Untargeted metabolomics reveals postoperative metabolic dynamics in hepatic cystic echinococcosis patients
Kahaer Tuerxun, Abudouxikuer Abudoumijiti, Zainuer Yusupu, Rousitaimujiang Yimamu, Ronghua Tang, Ziru Wang, Abudoukeyimu Yasheng, Irshat Ibrahim, Yuanquan Wu
Immunobiology.2025; 230(4): 153099. CrossRef
Radical-induced lipid oxidation produces a torrent of leukotriene-like agonists in severe asthma
Si-Yang Liu, Mikhail Linetsky, Abby Hite, Yu-Shiuan Cheng, Masaru Miyagi, Serena C. Zhu, Hong Zeng, Siqi Huang, Myra Qin, Emma Sintic, Carolyn M. Koutures, Abigail Meneses, Olivia R. Laniak, Sailaja Paruchuri, Lakshminarayan R. Teegala, Kaixi Cui, Fariba
Journal of Allergy and Clinical Immunology.2025;[Epub] CrossRef

2,474 View
75 Download
1 Web of Science
Crossref

Identification of the protease inhibitory domain of Trichinella spiralis novel cystatin (TsCstN): Thassanee Yuthithum, Orawan Phuphisut, Onrapak Reamtong, Nathamon Kosoltanapiwat, Salisa Chaimon, Porntida Kobpornchai, Charin Thawornkuno, Preeyarat Malaithong, Orathai Sawatdichaikul, Poom Adisakwattana; Parasites Hosts Dis 2024;62(3):330-341.
Published online August 26, 2024; DOI: https://doi.org/10.3347/PHD.24026

The Trichinella spiralis novel cystatin (TsCstN) inhibits cathepsin L (CatL) activity and inflammation of macrophages during lipopolysaccharide (LPS) induction. To identify the protease inhibitory region, this study applied an in silico modeling approach to simulate truncation sites of TsCstN (Ts01), which created four truncated forms, including TsCstN^∆1-39 (Ts02), TsCstN^∆1-71 (Ts03), TsCstN^{∆1-20, ∆73-117} (Ts04), and TsCstN^{∆1-20, ∆42-117} (Ts05). The superimposition of these truncates modeled with AlphaFold Colab indicated that their structures were more akin to Ts01 than those modeled with I-TASSER. Moreover, Ts04 exhibited the closest resemblance to the structure of Ts01. The recombinant Ts01 (rTs01) and truncated proteins (rTs02, rTs03, and rTs04) were successfully expressed in a prokaryotic expression system while Ts05 was synthesized, with sizes of approximately 14, 12, 8, 10, and 2.5 kDa, respectively. When determining the inhibition of CatL activity, both rTs01 and rTs04 effectively reduced CatL activity in vitro. Thus, the combination of the α1 and L1 regions may be sufficient to inhibit CatL. This study provides comprehensive insights into TsCstN, particularly regarding its protein function and inhibitory domains against CatL.

Citations

Citations to this article as recorded by

Systems biology of Haemonchus contortus – Advancing biotechnology for parasitic nematode control
Yuanting Zheng, Neil D. Young, Tao Wang, Bill C.H. Chang, Jiangning Song, Robin B. Gasser
Biotechnology Advances.2025; 81: 108567. CrossRef
Therapeutic potentials of Trichinella spiralis in immune disorders: From allergy to autoimmunity
Minkyoung Cho, Hak Sun Yu
Parasites, Hosts and Diseases.2025; 63(2): 123. CrossRef

2,596 View
71 Download
2 Web of Science
Crossref

Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells: Guihua Zhao, Lixin Zhang, Lisha Dai, Haozhi Xu, Chao Xu, Ting Xiao, Jun Li, Hui Sun, Beibei Zhou, Kun Yin; Korean J Parasitol 2022;60(4):241-246.
Published online August 24, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.4.241

Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.

Citations

Citations to this article as recorded by

Detection and genotyping of Toxoplasma gondii oocysts in environmental samples in Gaza, Palestine: A combined parasitological and molecular approach
Zuhair Dardona, Adnan Al Hindi, Mohamed Hafidi, Ali Boumezzough, Fadel Sharif, Samia Boussaa
One Health Bulletin.2025;[Epub] CrossRef
The development and excretion of Toxoplasma gondii oocyst manipulate the gut microbiota in its definitive host
Gui-Hua Zhao, Bei-Bei Zhou, Zhi-Heng Cao, Ting Xiao, Ya-Nan Li, Wen-Ju Zhu, Hang Sun, Huan-Huan Xie, Xiao-Man Xie, Jun-Mei Zhang, Qi Wang, Xin Zhang, Jin-Jing Xie, Hong-Jie Dong, Chao Xu, Kun Yin
Parasites & Vectors.2025;[Epub] CrossRef
Toxoplasma gondii alters gut microbiota and systemic metabolism in cats: A multi-omics approach
Ji-Xin Zhao, Xue-Yao Wang, Xuancheng Zhang, Lu-Yao Tang, Shi-Chen Xie, Yi-Han Lv, Zhi Zheng, Ying-Qian Gao, Jing Jiang, Xiao-Xuan Zhang, He Ma
The Veterinary Journal.2025; 314: 106455. CrossRef
Proteomics analysis of Toxoplasma gondii merozoites reveals regulatory proteins involved in sexual reproduction
Guihua Zhao, Hongjie Dong, Lisha Dai, Huanhuan Xie, Hang Sun, Junmei Zhang, Qi Wang, Chao Xu, Kun Yin
Microbial Pathogenesis.2024; 186: 106484. CrossRef

4,526 View
148 Download
3 Web of Science
Crossref

Brief Communication

Unexpected Presence of Blastocystis Subtype 1-3 DNA in Human Vaginal and Sperm Samples Coinfected with Trichomonas vaginalis: Guiehdani Villalobos, Fabiola Sanchez-Aguillon, Marcia Veronica Carmona-Maldonado, Nelly Raquel Gonzalez-Arenas, Eduardo Lopez-Escamilla, Rigoberto Hernandez-Castro, Mirza Romero-Valdovinos, Williams Arony Martinez-Flores, Juan Pablo Ramirez-Hinojosa, Pablo Maravilla, Fernando Martinez-Hernandez; Korean J Parasitol 2022;60(3):195-200.
Published online June 30, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.3.195

There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis. Blastocystis STs were identified in 6 of 28 vaginal swabs (21.4%) and in 3 of 7 sperm samples (42.8%). In both biological samples, STs 1-3 were found; one vaginal sample showed subtype co-infection with ST1 and ST3. High genetic variation was observed in the sequences obtained and no specific clustering in the phylogenetic trees was detected. Most of the haplotypes identified were placed far from the main dispersal centers. Our finding suggested that incorrect cleaning of the genital area or a contamination by combination of anal and vaginal intercourse.

Citations

Citations to this article as recorded by

Molecular Identification of Blastocystis Subtypes in the Cervix: A Study on Polish Patients
Barbara Suchońska, Adam Kaczmarek, Maria Wesołowska, Daniel Młocicki, Rusłan Sałamatin
Journal of Clinical Medicine.2025; 14(11): 3928. CrossRef
Genetic Variability of the Internal Transcribed Spacer and Pyruvate:Ferredoxin Oxidoreductase Partial Gene of Trichomonas vaginalis from Female Patients
Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocaña, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos
Microorganisms.2023; 11(9): 2240. CrossRef

3,592 View
141 Download
2 Web of Science
Crossref

Original Articles

Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin: Kai Zhang, Yucheng Liu, Guowu Zhang, Xifeng Wang, Zhiyuan Li, Yunxia Shang, Chengcheng Ning, Chunhui Ji, Xuepeng Cai, Xianzhu Xia, Jun Qiao, Qingling Meng; Korean J Parasitol 2022;60(2):117-126.
Published online April 20, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.2.117

Cystatin, a cysteine protease inhibitor found in many parasites, plays important roles in immune evasion. This study analyzed the molecular characteristics of a cystatin from Fasciola hepatica (FhCystatin) and expressed recombinant FhCystatin (rFhcystatin) to investigate the immune modulatory effects on lipopolysaccharide-induced proliferation, migration, cytokine secretion, nitric oxide (NO) production, and apoptosis in mouse macrophages. The FhCystatin gene encoded 116 amino acids and contained a conserved cystatin-like domain. rFhCystatin significantly inhibited the activity of cathepsin B. rFhCystatin bound to the surface of mouse RAW264.7 cells, significantly inhibited cell proliferation and promoted apoptosis. Moreover, rFhCystatin inhibited the expression of cellular nitric oxide, interleukin-6, and tumor necrosis factor-α, and promoted the expression of transforming growth factor-β and interleukin-10. These results showed that FhCystatin played an important role in regulating the activity of mouse macrophages. Our findings provide new insights into mechanisms underlying the immune evasion and contribute to the exploration of potential targets for the development of new drug to control F. hepatica infection.

Citations

Citations to this article as recorded by

Cystatin from the helminth Ascaris lumbricoides upregulates mevalonate and cholesterol biosynthesis pathways and immunomodulatory genes in human monocyte-derived dendritic cells
Nathalie Acevedo, Ana Lozano, Josefina Zakzuk, Kevin Llinás-Caballero, David Brodin, Peter Nejsum, Andrew R. Williams, Luis Caraballo
Frontiers in Immunology.2024;[Epub] CrossRef
Helminth-derived molecules: Pathogenic and pharmacopeial roles
Yu Zhang, Chunxiang Shen, Xinyi Zhu, Chiuan Yee Leow, Minjun Ji, Zhipeng Xu
The Journal of Biomedical Research.2024; 38(6): 547. CrossRef
An evolutionary molecular adaptation of an unusual stefin from the liver fluke Fasciola hepatica redefines the cystatin superfamily
Michal Buša, Zuzana Matoušková, Pavla Bartošová-Sojková, Petr Pachl, Pavlína Řezáčová, Ramon Marc Eichenberger, Peter Deplazes, Martin Horn, Saša Štefanić, Michael Mareš
Journal of Biological Chemistry.2023; 299(3): 102970. CrossRef
Type I Cystatin Derived from Fasciola gigantica Suppresses Macrophage-Mediated Inflammatory Responses
Pathanin Chantree, Mayuri Tarasuk, Parisa Prathaphan, Jittiporn Ruangtong, Mantana Jamklang, Sirilak Chumkiew, Pongsakorn Martviset
Pathogens.2023; 12(3): 395. CrossRef
Cystatins from the Human Liver Fluke Opisthorchis viverrini: Molecular Characterization and Functional Analysis
Amornrat Geadkaew-Krenc, Rudi Grams, Sinee Siricoon, Nanthawat Kosa, Dawid Krenc, Wansika Phadungsil, Pongsakorn Martviset
Pathogens.2023; 12(7): 949. CrossRef
Antigens from the Helminth Fasciola hepatica Exert Antiviral Effects against SARS-CoV-2 In Vitro
Judit Serrat, Clara Francés-Gómez, David Becerro-Recio, Javier González-Miguel, Ron Geller, Mar Siles-Lucas
International Journal of Molecular Sciences.2023; 24(14): 11597. CrossRef
Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits
Guoliang Chen, Guiting Pu, Liqun Wang, Yanping Li, Tingli Liu, Hong Li, Shaohua Zhang, Xuelin Wang, Xiaolei Liu, Xuenong Luo
Frontiers in Immunology.2023;[Epub] CrossRef
Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
Luis Miguel Flores-Velázquez, María Teresa Ruiz-Campillo, Guillem Herrera-Torres, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Rafael Zafra, Leandro Buffoni, Pablo José Rufino-Moya, Verónica Molina-Hernández, José Pérez
Frontiers in Veterinary Science.2023;[Epub] CrossRef

5,488 View
205 Download
9 Web of Science
Crossref

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein: So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2022;60(1):1-6.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.1

Abstract
PDF

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein—Acanthamoeba silent-information regulator 2-like protein (AcSir2)—was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 μM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 μM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

Citations

Citations to this article as recorded by

Pterostilbene: A natural neuroprotective stilbene with anti-Alzheimer's disease properties
Songlan Gao, Honglei Zhang, Na Li, Lijuan Zhang, Zhe Zhu, Changlu Xu
Journal of Pharmaceutical Analysis.2025; 15(4): 101043. CrossRef
Alzheimer’s Disease: A Review of Molecular Mechanisms and Therapeutic Implications by Targeting Sirtuins, Caspases, and GSK-3
Kalpana Pandya, Krishnashish Roul, Avanish Tripathi, Sateesh Belemkar, Anshuman Sinha, Meryem Erol, Devendra Kumar
ACS Chemical Neuroscience.2025; 16(12): 2178. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells
Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha
Acta Tropica.2024; 257: 107288. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef

4,721 View
252 Download
5 Web of Science
Crossref

Human Taeniasis and Cysticercosis and Related Factors in Phu Tho Province, Northern Vietnam: Vu Thi Lam Binh, Do Trung Dung, Hoang Quang Vinh, Van Hul Anke, Praet Nicolas, Dorny Pierre, Dermauw Veronique; Korean J Parasitol 2021;59(4):369-376.
Published online August 18, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.4.369

Abstract
PDF

Several factors presumed to facilitate the transmission of Taenia spp. were reported in Vietnam. We conducted a cross-sectional study taking questionnaires from 1,185 participants, and collecting 1,151 sera and 1,036 stool samples in northern Vietnam. Sera were examined for circulating antigens of Taenia solium cysticerci using ELISA, stools for Taenia eggs by Kato-Katz smear, and copro-antigens by ELISA. Ag-ELISA revealed 4.6% antigen positivity, indicating infection with viable cysticerci. Taenia eggs were detected in 1.5% of participants. Copro-antigens were found in 2.8% of participants. Eating raw meat and/or vegetables was significantly associated with the presence of copro-antigen (OR=8.6, 95% CI: 1.16-63.9, P=0.01). Considering the high taeniasis prevalence and the associated threat, public health attention should be given to treat the tapeworm carriers in the projected areas.

Citations

Citations to this article as recorded by

The burden of T. solium cysticercosis and selected neuropsychiatric disorders in Mocuba district, Zambézia province, Mozambique
Irene Langa, Fernando Padama, Noémia Nhancupe, Alberto Pondja, Delfina Hlashwayo, Lidia Gouveia, Dominik Stelzle, Clarissa Prazeres da Costa, Veronika Schmidt, Andrea S. Winkler, Emília Virgínia Noormahomed, Eduardo Torres
PLOS Neglected Tropical Diseases.2022; 16(7): e0010606. CrossRef

5,574 View
87 Download
1 Web of Science
Crossref

Brief Communication

Parvatrema duboisi (Digenea: Gymnophallidae) Life Cycle Stages in Manila Clams, Ruditapes philippinarum, from Aphae-do (Island), Shinan-gun, Korea: Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Seungwan Ryoo, Sooji Hong, Jeonggyu Lee, Hyemi Song, Jaeeun Cho, Deok-Gyu Kim, Hojong Jun, Min-Jae Kim, Eun Jeong Won, Eun-Taek Han, Eun-Hee Shin, Jong-Yil Chai; Korean J Parasitol 2021;59(1):83-88.
Published online February 19, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.1.83

Abstract
PDF

Life cycle stages, including daughter sporocysts, cercariae, and metacercariae, of Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974 (Digenea: Gymnophallidae) have been found in the Manila clam Ruditapes philippinarum from Aphaedo (Island), Shinan-gun, Jeollanam-do, Korea. The daughter sporocysts were elongated sac-like and 307-570 (av. 395) μm long and 101-213 (av. 157) μm wide. Most of the daughter sporocysts contained 15-20 furcocercous cercariae each. The cercariae measured 112-146 (av. 134) μm in total length and 35-46 (av. 40) μm in width, with 69-92 (av. 85) μm long body and 39-54 (av. 49) μm long tail. The metacercariae were 210-250 (av. 231) μm in length and 170-195 (av. 185) μm in width, and characterized by having a large oral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, strongly suggesting P. duboisi. The metacercariae were experimentally infected to ICR mice, and adults were recovered at day 7 post-infection. The adult flukes were morphologically similar to the metacercariae except in the presence of up to 20 eggs in the uterus. The daughter sporocysts and metacercariae were molecularly (ITS1-5.8S rDNA-ITS2) analyzed to confirm the species, and the results showed 99.8-99.9% identity with P. duboisi reported from Kyushu, Japan and Gochang, Korea. These results confirmed the presence of various life cycle stages of P. duboisi in the Manila clam, R. philippinarum, playing the role of the first as well as the second intermediate host, on Aphae-do (Island), Shinan-gun, Korea.

Citations

Citations to this article as recorded by

Spatial patterns of trematode-induced pits on bivalve skeletons: Challenges and prospects for research on parasite-host dynamics
Alexis Rojas, John Warren Huntley, Monica Caffara, Daniele Scarponi
The Holocene.2025; 35(12): 1259. CrossRef
Pearl formation associated with gymnophallid metacercariae in Mytilus galloprovincialis from the Northwestern Adriatic coast: Preliminary observations
Erica Marchiori, Francesco Quaglio, Giovanni Franzo, Ginevra Brocca, Sonja Aleksi, Pietrogiovanni Cerchier, Rudi Cassini, Federica Marcer
Journal of Invertebrate Pathology.2023; 196: 107854. CrossRef
Molecular phylogeny, distribution, and pathogenicity of a novel thraustochytrid protist in the Manila clam, Ruditapes philippinarum, on the west and south coasts of Korea
Biyun Zhao, Seung-Hyeon Kim, Dae-Woong Koh, S.D.N.K. Bathige, Kwang-Sik Choi, Kyung-Il Park
Aquaculture.2023; 575: 739779. CrossRef
Biotic and abiotic drivers affect parasite richness, prevalence and abundance inMytilus galloprovincialisalong the Northern Adriatic Sea
C. Bommarito, M. Wahl, D.W. Thieltges, C. Pansch, M. Zucchetta, F. Pranovi
Parasitology.2022; 149(1): 15. CrossRef
Effects of larval trematode parasitism on the reproductive capacity of Manila clam Ruditapes philippinarum in a sandy-mud tidal flat on the west coast of Korea
Young-Ghan Cho, Hye-Mi Lee, Jeong-Hwa Kim, Jong-Seop Shin, Hee-Do Jeung, Kwang-Sik Choi
Frontiers in Marine Science.2022;[Epub] CrossRef
Negative Impacts of Perkinsus olseni Infection in Manila Clam Ruditapes philippinarum Observed from Tidal Flats in Anmyeondo Island on the West Coast of Korea During Post-Spawning Period
Hye-Mi Lee, Kyung-Il Park, Hyun-Sung Yang, Kwang-Sik Choi
Ocean Science Journal.2021; 56(3): 307. CrossRef

5,371 View
118 Download
5 Web of Science
Crossref

Case Report

An Unusual Case of Extra-Enteric Blastocystosis in the Uterine Cervix: Yolanda Escutia-Guzman, Williams Arony Martinez-Flores, Joel Martinez-Oca?a, Ramon Martinez-Pimentel, Marisol Benitez-Ramirez, Fernando Martinez-Hernandez, Sara Arroyo-Escalante, Mirza Romero-Valdovinos, Guadalupe Erendira Orozco-Mosqueda, Pablo Maravilla; Korean J Parasitol 2020;58(5):571-576.
Published online October 22, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.5.571

Abstract
PDF

Extra-enteric infections by Blastocystis spp. have rarely been documented. Here, we report a case of extra-enteric blastocystosis in a patient with minimal cervicitis symptoms. A 47-year-old Hispanic female patient was attended in a primary health centre in Michoacan state, Mexico, for her routine gynaecological medical examination. As only symptom, she referred to a slight vaginal itching. The presence of several vacuolar-stages of Blastocystis spp. were identified by Papanicolaou staining; molecular identification was attempted by culture-PCR sequencing of a region of 18S gene from cervical and faecal samples obtained 2 months after cytological examination, even when patient declared that she tried self-medicating with vaginal ovules. Blastocystis ST1 was identified only in the faecal sample. The presence of Blastocystis spp. in the cervix of a patient with scarce symptomatology, demonstrates the extraordinary flexibility of this microorganism to adapt to new environments and niches.

Citations

Citations to this article as recorded by

Molecular Identification of Blastocystis Subtypes in the Cervix: A Study on Polish Patients
Barbara Suchońska, Adam Kaczmarek, Maria Wesołowska, Daniel Młocicki, Rusłan Sałamatin
Journal of Clinical Medicine.2025; 14(11): 3928. CrossRef
Unexpected Presence of Blastocystis Subtype 1-3 DNA in Human Vaginal and Sperm Samples Coinfected with Trichomonas vaginalis
Guiehdani Villalobos, Fabiola Sanchez-Aguillon, Marcia Veronica Carmona-Maldonado, Nelly Raquel Gonzalez-Arenas, Eduardo Lopez-Escamilla, Rigoberto Hernandez-Castro, Mirza Romero-Valdovinos, Williams Arony Martinez-Flores, Juan Pablo Ramirez-Hinojosa, Pab
The Korean Journal of Parasitology.2022; 60(3): 195. CrossRef

5,471 View
91 Download
2 Web of Science
Crossref

Original Articles

Seroprevalence of Tissue and Luminal Helminths among Patients in Hanoi Medical University Hospital, Vietnam, 2018: Nguyen Van De, Pham Ngoc Minh, Nguyen Ngoc Bich, Jong-Yil Chai; Korean J Parasitol 2020;58(4):387-392.
Published online August 25, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.4.387

Abstract
PDF

A serological survey was performed using ELISA to estimate the prevalence of tissue and luminal helminthic infections among hospital patients in Hanoi region, Vietnam. An overall seroprevalence of tissue and luminal helminthiases was 64.0% (95% CI 61.2-66.8) among 1,120 patients who visited Hanoi Medical University Hospital, Vietnam in 2018. The highest seroprevalence was observed against Toxocara spp. (59.0%), followed by Strongyloides stercoralis (46.3%), Gnathostoma spp. (25.5%), cysticercus (12.8%), Angiostrongylus cantonensis (10.5%), Fasciola spp. (11.1%), and Clonorchis sinensis (8.7%). Mono-infection by one species (11.1%) was lower than multiple infections (53.0%) (P<0.05). The seroprevalence in males (59.3%) was lower than in females (66.2%) but not statistically significant (P>0.05). Children (<15 years) revealed lower seroprevalence (34.0%) than adults (68.4%), and the age group 51-70 years revealed the highest seroprevalence (76.0%). Among the seropositive patients, eosinophilia (≥8.0%) was noted in 80.2%. The present results suggested active transmission of various tissue and luminal helminths among people in Hanoi, Vietnam.

Citations

Citations to this article as recorded by

Seroprevalence and associated risk factors of human fascioliasis in Yen Bai and Thanh Hoa provinces, Vietnam
Thao Thi Bich Nguyen, Hafid Dahma, Veronique Dermauw, Dung Thi Bui, Bertrand Losson, Pierre Dorny, Dung Trung Do, Olivier Vandenberg
Parasitology International.2026; 112: 103219. CrossRef
Toxocara seroprevalence among suspected patients across different geographical regions in Vietnam: A retrospective study
Trinh Thi Que, Tran Van Chieu, Trieu Thuy Anh, Pham Van Ngai, Pham Van Tran, Nguyen Thai Son, Do Ngoc Anh
Journal of Microbiology, Immunology and Infection.2025;[Epub] CrossRef
The seroprevalence of toxocariasis and related risk factors in children in Ho Chi Minh City, Vietnam: results from a school-based cross-sectional study
Thieu V Ha, Tien T N Vo, Duy K H Dang, Y M L Tran, Thanh V Kim, Duc H Le, Lan-Anh T Do, Hong K Tang
Transactions of The Royal Society of Tropical Medicine and Hygiene.2024; 118(6): 384. CrossRef
Fasciola spp. in Southeast Asia: A systematic review
Vinh Hoang Quang, Bruno Levecke, Dung Do Trung, Brecht Devleesschauwer, Binh Vu Thi Lam, Kathy Goossens, Katja Polman, Steven Callens, Pierre Dorny, Veronique Dermauw, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2024; 18(1): e0011904. CrossRef
Fascioliasis, una zoonosis emergente en Perú y el mundo: factores asociados a la infección en niños de edad escolar
A. Valderrama–Pomé
Revista de la Facultad de Medicina Veterinaria y de Zootecnia.2023;[Epub] CrossRef
Prevalence of Strongyloides in Southeast Asia: a systematic review and meta-analysis with implications for public health and sustainable control strategies
Abigail Hui En Chan, Teera Kusolsuk, Dorn Watthanakulpanich, Wallop Pakdee, Pham Ngoc Doanh, Azlin Mohd Yasin, Paron Dekumyoy, Urusa Thaenkham
Infectious Diseases of Poverty.2023;[Epub] CrossRef
Seroprevalence of toxocariasis among allergic patients in Kuwait and its association with eosinophilia
Mohammad Al-Awadhi, Wafaa Jamal
Parasite Epidemiology and Control.2022; 18: e00260. CrossRef
Human Fascioliasis: Current Epidemiological Status and Strategies for Diagnosis, Treatment, and Control

Maria Alejandra Caravedo, Miguel Cabada
Research and Reports in Tropical Medicine.2020; Volume 11: 149. CrossRef

5,554 View
112 Download
9 Web of Science
Crossref

Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process: Junjie Fan, Hongye Wu, Kai Li, Xunuo Liu, Qingqing Tan, Wenqiao Cao, Bo Liang, Bin Ye; Korean J Parasitol 2020;58(3):287-299.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.287

Cystic echinococcosis (CE) is a zoonotic infection caused by Echinococcus granulosus larvae. It seriously affects the development of animal husbandry and endangers human health. Due to a poor understanding of the cystic fluid formation pathway, there is currently a lack of innovative methods for the prevention and treatment of CE. In this study, the protoscoleces (PSCs) in the encystation process were analyzed by high-throughput RNA sequencing. A total of 32,401 transcripts and 14,903 cDNAs revealed numbers of new genes and transcripts, stage-specific genes, and differently expressed genes. Genes encoding proteins involved in signaling pathways, such as putative G-protein coupled receptor, tyrosine kinases, and serine/threonine protein kinase, were predominantly up-regulated during the encystation process. Antioxidant enzymes included cytochrome c oxidase, thioredoxin glutathione, and glutathione peroxidase were a high expression level. Intriguingly, KEGG enrichment suggested that differentially up-regulated genes involved in the vasopressin-regulated water reabsorption metabolic pathway may play important roles in the transport of proteins, carbohydrates, and other substances. These results provide valuable information on the mechanism of cystic fluid production during the encystation process, and provide a basis for further studies on the molecular mechanisms of growth and development of PSCs.

Citations

Citations to this article as recorded by

First comparative proteomic and in vitro behavioral study of Echinococcus granulosus metacestodes in Felis catus
Andrea Maglioco, Vanesa V. Miana, María Pía Valacco, Facundo A. Agüero, María Laura Gertiser, Héctor G. Avila, Melisa S. Barbery Venturi, Oscar Jensen, Alejandra Y. Juárez Valdez, Elio A. Prieto González, Alicia G. Fuchs
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Genome-wide transcriptome analysis of Echinococcus multilocularis larvae and germinative cell cultures reveals genes involved in parasite stem cell function
Michaela Herz, Magdalena Zarowiecki, Leonie Wessels, Katharina Pätzel, Ruth Herrmann, Christiane Braun, Nancy Holroyd, Thomas Huckvale, Monika Bergmann, Markus Spiliotis, Uriel Koziol, Matthew Berriman, Klaus Brehm
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
In vitro and in silico scolicidal effect of sanguinarine on the hydatid cyst protoscoleces
Elham Hassanzadeh, Shahram Khademvatan, Behzad Jafari, Abbas Jafari, Elham Yousefi, Sheikh Arslan Sehgal
PLOS ONE.2023; 18(10): e0290947. CrossRef
Transcriptome analysis of Echinococcus granulosus sensu stricto protoscoleces reveals differences in immune modulation gene expression between cysts found in cattle and sheep
Ismael Pereira, Christian Hidalgo, Caroll Stoore, María Soledad Baquedano, Carolina Cabezas, Macarena Bastías, Aníbal Riveros, Claudio Meneses, Martín Cancela, Henrique Bunselmeyer Ferreira, Leonardo Sáenz, Rodolfo Paredes
Veterinary Research.2022;[Epub] CrossRef
Chromosome-scale Echinococcus granulosus (genotype G1) genome reveals the Eg95 gene family and conservation of the EG95-vaccine molecule
Pasi K. Korhonen, Liina Kinkar, Neil D. Young, Huimin Cai, Marshall W. Lightowlers, Charles Gauci, Abdul Jabbar, Bill C. H. Chang, Tao Wang, Andreas Hofmann, Anson V. Koehler, Junhua Li, Jiandong Li, Daxi Wang, Jiefang Yin, Huanming Yang, David J. Jenkins
Communications Biology.2022;[Epub] CrossRef
The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis
Raúl J. Bobes, Karel Estrada, Diana G. Rios-Valencia, Arturo Calderón-Gallegos, Patricia de la Torre, Julio C. Carrero, Alejandro Sanchez-Flores, Juan P. Laclette
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Evolutionary Adaptations of Parasitic Flatworms to Different Oxygen Tensions
José de Jesús Martínez-González, Alberto Guevara-Flores, Irene Patricia del Arenal Mena
Antioxidants.2022; 11(6): 1102. CrossRef
Bioinformatics analysis and experimental verification of Notch signalling pathway-related miRNA–mRNA subnetwork in extracellular vesicles during Echinococcus granulosus encystation
Jian Gao, Xuan Zhou, Ling Liu, Guodong Lv, Qiulian Hou, Xiaofan Zhang, Yujuan Shen
Parasites & Vectors.2022;[Epub] CrossRef
iTRAQ-based comparative proteomic analysis in different developmental stages ofEchinococcus granulosus
Xin Li, Song Jiang, Xuhai Wang, Wenqiao Hui, Bin Jia
Parasite.2021; 28: 15. CrossRef
Genome-wide transcriptome analysis of the early developmental stages of Echinococcus granulosus protoscoleces reveals extensive alternative splicing events in the spliceosome pathway
Mohammad Ali Mohammadi, Majid Fasihi Harandi, Donald P. McManus, Mehdi Mansouri
Parasites & Vectors.2021;[Epub] CrossRef
Synergism therapeutic and immunoregulatory effects of Albendazole + rAd-mIL-28B against Echinococcosis in experiment-infected mice with protoscoleces
Yan Zhang, Jianghua Wang, Qingxia Yang, Zhi Li, Xiaoying Xu, Chong Chen, Zongjie Hou, Qi He, Li Sheng, Xingming Ma, Yanping Luo, Alessandra Morassutti
PLOS Neglected Tropical Diseases.2021; 15(11): e0009927. CrossRef

8,626 View
130 Download
11 Web of Science
Crossref

Comprehensive Account on Prevalence and Characteristics of Hydatid Cysts in Livestock from Pakistan: Naunain Mehmood, Muhammad Arshad, Haroon Ahmed, Sami Simsek, Hira Muqaddas; Korean J Parasitol 2020;58(2):121-127.
Published online April 30, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.2.121

Abstract
PDF

Pakistan is at intersection of hyperendemic regions for hydatidosis. Current study aimed to determine the prevalence of hydatid cysts and cyst characteristics in different intermediate hosts (sheep, goats, cattle and buffaloes) across the 4 provinces of Pakistan. A total of 991 sheep, 1,478 goats, 1,602 cattle and 1,343 buffaloes were examined for presence of hydatid cysts during 2 years (January 2016-December 2018). Differences in frequency of hydatidosis were observed with highest overall prevalence in buffaloes (11.9%) and sheep (11.5%). Highest prevalence and burden of infection were observed in older age animals (23.8%, 9.78±0.49) and females (26.5%, 12.53±0.67). Data for seasonal prevalence alluded to year-round presence of disease with non-significant statistical difference. Organ predilection indicated liver as the most preferred site of cyst localization followed mainly by lungs. An over-dispersion pattern was observed in all infected animals as majority of cysts belonged <10 cysts per infected host category. Highest percentage of fertile cysts was observed in liver of sheep. Interestingly, solitary form of cysts had higher fertility rate than multiple form. Amid lack of data and wide gap of knowledge, this study would try to fill up the lacunae regarding this neglected tropical disease. Extensive rearing of livestock, unregulated official slaughter and home slaughtering have played role in adaptability of E. granulosus in Pakistan.

Citations

Citations to this article as recorded by

Epidemiological and genotypic assessment of cystic echinococcosis in ruminant populations of Northern Punjab, Pakistan: a neglected zoonotic disease
Sakandar Khan, Jo Cable, Numair Masud, Frank Hailer, Muhammad Younus, Naveed Hussain, Muhammad Asif Idrees, Muhammad Imran Rashid, Haroon Akbar
Parasitology Research.2025;[Epub] CrossRef
Frequency and Sequence Analysis of Echinococcus granulosus Sensu Lato Isolated From Buffaloes in Northern Iran
Fariborz Ghanbari, Keyhan Ashrafi, Majid Fasihi Harandi, Somayeh Abbaszadeh, Asieh Ashouri, Elham Hajialilo, Meysam Sharifdini
Veterinary Medicine and Science.2025;[Epub] CrossRef
Cancer on Hold, Infections on the Rise: The Unseen Pandemic Effect on Thoracic Surgery in the Developing World
Muhammad Imran, Elham Shakil, Shehryar Khan, Hira Bakhtiar Khan, Maha Wazir, Farhan Ullah, Dawood Tahir, Muhammad Moeed, Qaidar Alizai, Imran Tahir
Cureus.2025;[Epub] CrossRef
Investigating the Epidemiology and Socioecological Dynamics of Hydatid-like Cysts Within a Specific Endemic District
Muhammad Irfan Khan, Rahmat Ali, Dejun Ji, Wei Lan, Ahmed A. Saleh, Shahab Ur Rehman, Muhammad Shuaib, Shah Zeb Ahmad, Hussain, Xi Wu
Animals.2025; 15(11): 1617. CrossRef
Prevalence of Hydatidosis (Echinococcus granulosus) in Slaughtered Ruminants in and out of Abattoir in Duhok Area and its Correlation with Some Variables.
Rekesh S. Habib, Adnan M. Abdullah
Zanin Journal of Science and Engineering.2025; 1(2): 45. CrossRef
Molecular characterization and haplotypes of hydatid cyst isolates collected from humans and ruminants in Setif Province (northeast of Algeria) based on mitochondrial cytochrome C oxidase subunit 1 (mt-CO1) gene sequences
Amina Kheninef, Figen Celik, Lynda Aissaoui, Sami Simsek
Parasitology Research.2024;[Epub] CrossRef
The role of cattle in the transmission of cystic echinococcosis in a highly endemic area of the Peruvian Andes
J. Raúl Lucas, Miguel Cabrera, Néstor Falcón, Orlando Lucas, Alejandro Rodríguez, Daphne Ramos, Cesar Gavidia
Veterinary Parasitology: Regional Studies and Reports.2024; 51: 101021. CrossRef
Molecular Characterization and Phylogenetic Analysis of Fasciola gigantica Based on ITS-1 Genetic Marker and copro-epidemiological Survey from Ruminants of Pakistan
Naunain Mehmood, Shafiah Zulfiqar, Hira Muqaddas, Muhammad Hasnain, Yadong Zheng, Habtye Bisetegn, Fahad Ahmed, Zafar Iqbal Khan, Furhan Iqbal
Acta Parasitologica.2024; 69(4): 1866. CrossRef
Leading report regarding the molecular epidemiology of Taenia hydatigena from Pakistan and global overview of the genetic diversity and population structure of the parasite
Naunain Mehmood, Hira Muqaddas, Aqsa Ashraf, Mahnoor Aslam, Maryam Khan, Madiha Fatima, Rahman Qadir, Samir Ibenmoussa, Turki M. Dawoud, Muhammad Irfan Ullah, Furhan Iqbal
Comparative Immunology, Microbiology and Infectious Diseases.2024; 114: 102248. CrossRef
First molecular report of Moniezia expansa in small ruminants of Pakistan with epidemiological insight
Hira Muqaddas, Naunain Mehmood, Maher Nigar, Farhana Yousaf, Kainat Farooq Khokhar, Saba Kousar, Mahnoor Aslam, Zafar Iqbal Khan, Ioannis A. Giantsis, Ayman A. Swelum, Furhan Iqbal, Hudson Alves Pinto
PLOS ONE.2024; 19(12): e0314343. CrossRef
An Epidemiological Survey to Investigate the Prevalence of Cystic Echinococcosis in Slaughtered Bovine Hosts in Punjab, Pakistan
Sadia Saleem, Haroon Ahmed, Kaleem Imdad, Jing Zhang, Jianping Cao
Veterinary Sciences.2023; 10(1): 40. CrossRef
First report of Echinococcus ortleppi and genotype G6 of E. canadensis cluster from southern Punjab, Pakistan and a global overview on genetic structure and host adaptation of E. ortleppi
Hira Muqaddas, Naunain Mehmood, Urmas Saarma, Ammarah Usman, Fahad Ahmed, Antonio Varcasia, Maria Francesca Sini, Muhammad Irfan Ullah
Acta Tropica.2023; 244: 106951. CrossRef
Epidemiological and histomorphic studies in sheep infected with hydatid cyst in Taif area
Jamila Al Malki, Nibal Ahmed
Saudi Journal of Biological Sciences.2022; 29(2): 886. CrossRef
Genetic structure and phylogeography of Echinococcus granulosus sensu stricto genotypes G1 and G3 in Pakistan and other regions of the world based on nad5 gene
Naunain Mehmood, Hira Muqaddas, Muhammad Irfan Ullah, Urmas Saarma, Antonio Varcasia
Infection, Genetics and Evolution.2022; 98: 105223. CrossRef
Pattern and Major Reasons of Cattle Red Offal Condemnation in the Slaughterhouse of the Arid Region of El Oued (Algeria)
Ahmed Hadef, Souad Righi, Mehdi Boucheikhchoukh, Chams Eddine Bouzid
Agriculture.2022; 12(9): 1377. CrossRef
Meta-analysis of the prevalence of bovine cystic echinococcosis in China during decade
Xin-Bo Yang, Xiang-Zhu Meng, Yan Zhao, Jin-Ping Zhao, Chao Chen, Ya Qin, Yuan Zhang, Quan Zhao
Research in Veterinary Science.2022; 152: 465. CrossRef
Productivity and the Qualitative Response of Sorghum to Different Planting Patterns and Various Cultivars
Muhammad Asim, Muhammad Israr Khan, Abdul Rab
Journal of Soil, Plant and Environment .2022; 1(1): 89. CrossRef
Phylogenetics and Pathology of Hydatid Disease in Slaughtered Buffaloes of North India
Aman D. Moudgil, Anil K. Nehra, Vikas Nehra, Rakshita Sharma, Sukhdeep Vohra, Pallavi Moudgil
Acta Parasitologica.2021; 66(3): 899. CrossRef
Cystic echinococcosis in sheep and goats of Lebanon
Gaelle Joanny, Naunain Mehmood, Giorgia Dessì, Claudia Tamponi, Francesca Nonnis, Chadi Hosri, Urmas Saarma, Antonio Varcasia, Antonio Scala
Parasitology.2021; 148(7): 871. CrossRef
Evaluation of zoonotic platyhelminthe infections identified in slaughtered livestock in Iran, 2015–2019
Behzad Kiani, Christine M. Budke, Ebrahim Shams Abadi, Soheil Hashtarkhani, Amene Raouf Rahmati, Mostafa AkbarPour, Mehdi Zarean, Bibi Razieh Hosseini Farash, Fatemeh Kiani, Elham Moghaddas
BMC Veterinary Research.2021;[Epub] CrossRef
Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep
Christian Hidalgo, Caroll Stoore, María Soledad Baquedano, Ismael Pereira, Carmen Franco, Marcela Hernández, Rodolfo Paredes
Veterinary Research.2021;[Epub] CrossRef
Echinococcoses in Iran, Turkey, and Pakistan: Old Diseases in the New Millennium
Mehdi Borhani, Saeid Fathi, Enayat Darabi, Fatemeh Jalousian, Sami Simsek, Haroon Ahmed, Harun Kaya Kesik, Seyed Hossein Hosseini, Thomas Romig, Majid Fasihi Harandi, Iraj Mobedi
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Genetic variability and diversity of Echinococcus granulosus sensu lato in human isolates of Pakistan based on cox1 mt-DNA sequences (366bp)
Hira Muqaddas, Naunain Mehmood, Muhammad Arshad
Acta Tropica.2020; 207: 105470. CrossRef
Comprehensive study based on mtDNA signature (nad1) providing insights on Echinococcus granulosus s.s. genotypes from Pakistan and potential role of buffalo-dog cycle
Naunain Mehmood, Hira Muqaddas, Muhammad Arshad, Muhammad Irfan Ullah, Zafar Iqbal Khan
Infection, Genetics and Evolution.2020; 81: 104271. CrossRef
Cystic Echinococcosis in Pakistan: A Review of Reported Cases, Diagnosis, and Management
Aisha Khan, Haroon Ahmed, Huma Khan, Sadia Saleem, Sami Simsek, Enrico Brunetti, Muhammad Sohail Afzal, Tommaso Manciulli, Christine M. Budke
Acta Tropica.2020; 212: 105709. CrossRef

8,380 View
176 Download
27 Web of Science
Crossref

Brief Communication

Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan: Rosline James Muku, Hong-Bin Yan, John Asekhaen Ohiolei, Abubakar Ahmed Saaid, Sara Ahmed, Wan-Zhong Jia, Bao-Quan Fu; Korean J Parasitol 2020;58(1):93-97.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.93

The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to T. hydatigena has large veterinary and economic drawbacks. Like other taeniids, e.g., Echinococcus, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of T. hydatigena, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) mitochondrial genes. The isolates were correctly identified as T. hydatigena with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the nad1 and cox1 genes, respectively. The results suggest the presence of unique T. hydatigena haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of T. hydatigena in Africa, this report represents the first insights into the genetic variation of T. hydatigena in Sudan and constitutes useful data.

Citations

Citations to this article as recorded by

Identification of protein extracts from cysticercus tenuicollis using SDS–PAGE method collected from sheep in slaughterhouse, Kerbala Provenance, Iraq
Jihad Talib Obead, Bariq Abd Alameer Mohammed, Hayder Talib Mahdi, Ihsan Mohammed Sulbi
Eximia.2025; 14(1): 211. CrossRef
PREVALENCE AND ASSOCIATED RISK FACTORS OF Cysticercus tenuicollis IN THE SMALL RUMINANTS IN DIFFERENT DISTRICTS OF PUNJAB AND KPK PROVINCES OF PAKISTAN
W Qamar, R.Z. Abbas, M Imran, M.S. Mahmood
The Journal of Animal and Plant Sciences.2025; (4): 935. CrossRef
Molecular Identification of Cryptic Cysticercosis: Taenia spp. in Wild and Domestic Intermediate Hosts in Kazakhstan
Vladimir Kiyan, Ainura Smagulova, Rabiga Uakhit, Carlos Hermosilla, Lyudmila Lider, Karina Jazina, Nurassyl Manapov
Diversity.2025; 17(9): 655. CrossRef
Acute visceral cysticercosis in a Black Bengal goat: A case report of hepatitis cysticercosa and pneumonitis cysticercosa
Vinay Kumar S.D., Sree Lakshmi P., Neha, Avantika Srivastav, Manohar S., Veena R. Sutar, Pawan Kumar, Rajveer Singh Pawaiya, Vidya Singh
Veterinary Parasitology: Regional Studies and Reports.2025; 66: 101378. CrossRef
Genetic diversity and haplotypes of Cysticercus tenuicollis isolates from slaughtered sheep and goats in Elazig and Bingol provinces of Turkey
Sirvan Karakoc, Harun Kaya Kesik, Figen Celik, Muhammed Ahmed Selcuk, Seyma Gunyakti Kilinc, Sami Simsek
Veterinary Medicine and Science.2024;[Epub] CrossRef
Leading report regarding the molecular epidemiology of Taenia hydatigena from Pakistan and global overview of the genetic diversity and population structure of the parasite
Naunain Mehmood, Hira Muqaddas, Aqsa Ashraf, Mahnoor Aslam, Maryam Khan, Madiha Fatima, Rahman Qadir, Samir Ibenmoussa, Turki M. Dawoud, Muhammad Irfan Ullah, Furhan Iqbal
Comparative Immunology, Microbiology and Infectious Diseases.2024; 114: 102248. CrossRef
Molecular Identification of Selected Cervid Helminths in Supplementarily Fed European Bison Population
Magdalena Świsłocka-Cutter, Rafał Kowalczyk, Anetta Borkowska, Tomasz Kamiński, Marta Kołodziej-Sobocińska, María Eugenia López-Arellano
Journal of Parasitology Research.2024;[Epub] CrossRef
Epidemiology of Cysticercus tenuicollis in Sheep and Goats in the Tropics: A Systematic Review
Prudentia Yensi Lawan, Aziwo Tatanja Niba, Julius Awah-Ndukum, Francesca Mancianti
Veterinary Medicine International.2024;[Epub] CrossRef
PCR‐RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group
John Asekhaen Ohiolei, Hong‐Bin Yan, Li Li, Mughees Aizaz Alvi, Rosline James Muku, Yao‐Dong Wu, Nian‐Zhang Zhang, Wen‐Hui Li, Ai‐Min Guo, Xue‐Lin Wang, Bao‐Quan Fu, Wan‐Zhong Jia
Transboundary and Emerging Diseases.2022; 69(4): 2390. CrossRef
Prevalence and genetic variance of Taenia hydatigena in goats and sheep from northern Ghana: Preliminary data on a globally neglected livestock parasite
Francis Addy, Gideon Adu-Bonsu, Angelina Evelyn Dickson, Derrick Dankwa, Randy Aryee, Osman A. Dufailu, Thomas Romig, Marion Wassermann
Veterinary Parasitology: Regional Studies and Reports.2022; 30: 100711. CrossRef
Molecular identification of Taenia hydatigena from domestic and free-living animals in Slovakia, Central Europe
Júlia Jarošová, Daniela Antolová, Adriana Iglodyová, Alžbeta Königová, Michaela Urda Dolinská, Bronislava Víchová
Parasitology Research.2022; 121(5): 1345. CrossRef
Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia
Sayed Ajmal Qurishi, Hong-Bin Yan, Li Li, John Aeskhaen Ohiolei, Mughees Aizaz Alvi, Lin-Sheng Zhang, Ha Da, Hong-Mei Qiao, Nigus Abebe Shumuye, Bao Hua, Bing-Xin Bai, Wen-Jun Tian, Ju-Mei Xu, Bao-Quan Fu, Wan-Zhong Jia
Parasitology Research.2022; 121(12): 3455. CrossRef
A new molecular nomenclature for Taenia hydatigena: mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions
John Asekhaen Ohiolei, Hong-Bin Yan, Li Li, Wen-Hui Li, Yao-Dong Wu, Mughees Aizaz Alvi, Nian-Zhang Zhang, Bao-Quan Fu, Xue-Lin Wang, Wan-Zhong Jia
Parasitology.2021; 148(3): 311. CrossRef
Molecular Characterization and Phylogeny of Taenia hydatigena and Echinococcus granulosus from Iranian Sheep and Cattle Based on COX1 Gene
Vahid Raissi, Soudabeh Etemadi, Nasrin Sohrabi, Omid Raiesi, Mehdi Shahraki, Alireza Salimi-Khorashad, Asmaa Ibrahim
Current Microbiology.2021; 78(4): 1202. CrossRef
Molecular characterization ofCysticercus tenuicollisisolates from sheep in the Nile Delta, Egypt and a review onTaenia hydatigenainfections worldwide
Ibrahim Abbas, El-Sayed El-Alfy, Elisabeth Janecek-Erfurth, Christina Strube
Parasitology.2021; 148(8): 913. CrossRef
Prevalence and risk factors of Taenia hydatigena in dogs, pigs, and cattle in the Central Highlands of Vietnam
Dinh Ng-Nguyen, Thai Van Nguyen, Trong Van Nguyen, Hieu Quoc Nguyen, Van-Anh Thi Nguyen
Parasitology Research.2021; 120(9): 3245. CrossRef
Preliminary information on the prevalence and molecular description of Taenia hydatigena isolates in Pakistan based on mitochondrial cox1 gene
Mughees Aizaz Alvi, John Asekhaen Ohiolei, Muhammad Saqib, Li Li, Nehaz Muhammad, Muhammad Haleem Tayyab, Warda Qamar, Anum Aizaz Alvi, Yao-Dong Wu, Xiu-Rong Li, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Infection, Genetics and Evolution.2020; 85: 104481. CrossRef

8,783 View
149 Download
16 Web of Science
Crossref

Case Report

Case Report of Urinary Schistosomiasis in a Returned Traveler in Korea: Yangkyu Lee, Hyun Beom Song, Bong-Kwang Jung, Gheeyoung Choe, Min-Ho Choi; Korean J Parasitol 2020;58(1):51-55.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.51

Abstract
PDF

A 23-year-old Korean woman with a residence history in Kenya and Malawi for about 2 years presented with gross hematuria for 1 month. Blood tests were within normal range except eosinophilia. Asymmetrically diffuse wall thickening and calcification were observed at the urinary bladder on CT. Multiple erythematous nodular lesions were observed in the cystoscopy and transurethral resection was done. Numerous eggs of Schistosoma haematobium with granulomatous inflammation were observed in the submucosal layer of the bladder. The patient was diagnosed with schistosomiasis-related cystitis and treated with praziquantel (40 mg/kg/day) twice before and after transurethral resection. This case suggests that S. haematobium infection should be considered as a cause of hematuria in Korea when the patient had a history of traveling endemic areas of schistosomiasis.

Citations

Citations to this article as recorded by

Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Diagnosis and management of complicated urogenital schistosomiasis: a systematic review of the literature
Tommaso Manciulli, Davide Marangoni, Joaquin Salas-Coronas, Cristina Bocanegra, Joachim Richter, Federico Gobbi, Leonardo Motta, Andrea Minervini, Alessandro Bartoloni, Lorenzo Zammarchi
Infection.2023; 51(5): 1185. CrossRef
Bladder stones in a closed diverticulum caused by Schistosoma mansoni: A case report
Mohammad A Alkhamees
World Journal of Clinical Cases.2020; 8(19): 4475. CrossRef
Parasitological Observation in Schoolchildren with Urogenital Schistosomiasis Following Treatment with Three Different Brands of Praziquantel
Yan Jin, Seungman Cha, Jinmoo Lee, Mousab Siddig Elhag, Sung-Tae Hong, Young-Ha Lee
Journal of Korean Medical Science.2020;[Epub] CrossRef

10,305 View
183 Download
4 Web of Science
Crossref

Original Articles

Seroprevalence of Sarcocystis falcatula in Two Islands of Malaysia using Recombinant Surface Antigen 4: Tengku-Idris Tengku Idzzan Nadzirah, Fong Mun Yik, Lau Yee Ling; Korean J Parasitol 2020;58(1):1-5.
Published online February 29, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.1.1

Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis.

Citations

Citations to this article as recorded by

Sarcocystis infection in domestic and wild avian hosts: Inseparable flight partners
Petras Prakas, Rafael Calero-Bernal, Jitender P. Dubey
Veterinary Parasitology.2025; 335: 110413. CrossRef

6,429 View
176 Download
1 Web of Science
Crossref

Excretion of Toxoplasma gondii oocysts from Feral Cats in Korea: Kyu-Sung Ahn, Ah-Jin Ahn, Sang-ik Park, Woon-Mok Sohn, Jae-han Shim, Sung-Shik Shin; Korean J Parasitol 2019;57(6):665-670.
Published online December 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.6.665

Abstract
PDF

Sporulated oocysts from the feces of infected cats with Toxoplasma gondii can cause detrimental disease in both humans and animals. To investigate the prevalence of feral cats that excrete T. gondii oocysts in the feces, we examined fecal samples of 563 feral cats over a 3-year period from 2009 to 2011. Oocysts of T. gondii excreted into the feces were found from 4 of 128 cats in 2009 (3.1%) and one of 228 (0.4%) in 2010 while none of the 207 cats in 2010 were found positive with oocysts in their feces, resulting in an overall prevalence rate of 0.89% (5/563) between 2009 and 2011. Among the 5 cats that tested positive with T. gondii oocysts, 4 of the cats were male and 1 was a female with an average body weight of 0.87 kg. Numerous tissue cysts of 60 ?m in diameter with thin (<0.5 ?m) cyst walls were found in the brain of one of the 5 cats on necropsy 2 months after the identification of oocysts in the feces. A PCR amplification of the T. gondii-like oocysts in the feces of the positive cats using the primer pairs Tox-5/Tox-8 and Hham34F/Hham3R confirmed the presence of T. gondii oocysts in the feces. This study provides a good indication of the risk assessment of feral cats in the transmission of T. gondii to humans in Korea.

Citations

Citations to this article as recorded by

Toxoplasmosis, Prevalence of Toxoplasma gondii Parasitic in Domestic Cats in Al-Marj City - Libya
Faisal Fathalla Mohammed , Munthir Salah Alasbali
مجلة العلوم والدراسات الإنسانية - كلية الآداب والعلوم – المرج .2024; (77): 1. CrossRef
The detection and phylogenetic characterization of Cryptosporidium, Cystoisospora, and Giardia duodenalis of cats in South Korea
Chi Sun Yun, Bo-Youn Moon, Kichan Lee, Su Min Kang, Bok-Kyung Ku, Mi-Hye Hwang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Prevalence of Toxoplasma gondii Measured by Western Blot, ELISA and DNA Analysis, by PCR, in Cats of Western Mexico
María de la Luz Galván-Ramírez, Claudia Charles-Niño, César Pedroza-Roldán, Carolina Salazar-Reveles, Karen Lissete Ocampo-Figueroa, Laura Roció Rodríguez-Pérez, Varinia Margarita Paez-Magallán
Pathogens.2022; 11(1): 109. CrossRef
Seroprevalence of Toxoplasma gondii in domestic cats in tropical region of Serra da Tiririca State Park, Niterói, Rio de Janeiro
R.L. Freitas, A. Consalter, A.F.S. Spyrides, P.B.A. Dória, G.L. Albuquerque, S.F. Bruno, C.G. Barbosa, A.M.R. Ferreira
Arquivo Brasileiro de Medicina Veterinária e Zootecnia.2022; 74(2): 319. CrossRef
Prevalence of Zoonotic Pathogens in Domestic and Feral Cats in Shanghai, with Special Reference to Salmonella
Halim A, Bhekharee AK, Feng M, Cheng X, Halim M
Journal of Health Care and Research.2021; 2(1): 1. CrossRef
All about toxoplasmosis in cats: the last decade
J.P. Dubey, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, Y.R. Yang, C. Su
Veterinary Parasitology.2020; 283: 109145. CrossRef
Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea
Ju Yeong Kim, You Shine Kwak, In-Yong Lee, Tai-Soon Yong
The Korean Journal of Parasitology.2020; 58(3): 327. CrossRef
Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
The Korean Journal of Parasitology.2020; 58(3): 257. CrossRef
Genetic Analysis of Zoonotic Gastrointestinal Protozoa and Microsporidia in Shelter Cats in South Korea
Dongmi Kwak, Min-Goo Seo
Pathogens.2020; 9(11): 894. CrossRef

11,109 View
202 Download
7 Web of Science
Crossref

Proteomic Analysis on Exosomes Derived from Patients’ Sera Infected with Echinococcus granulosus: Wen Wang, Xiaojing Zhou, Fang Cui, Chunli Shi, Yulan Wang, Yanfei Men, Wei Zhao, Jiaqing Zhao; Korean J Parasitol 2019;57(5):489-497.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.489

Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.

Citations

Citations to this article as recorded by

Progresses and challenges in Strongyloides spp. proteomics
Natalia Tiberti, Marcello Manfredi, Chiara Piubelli, Dora Buonfrate
Philosophical Transactions of the Royal Society B: Biological Sciences.2024;[Epub] CrossRef
A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease
Jing Xiao, Jian-Wen Wu, Yun-Zhuo Xin, Dong Song, Xiao-ping Gao, Mei Yin, Wei Zhao, Fu-lin Liu, Hao Wang, Jie Wang, Jia-Qing Zhao
Parasitology Research.2024;[Epub] CrossRef
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response
María Celeste Nicolao, Christian Rodriguez Rodrigues, Magalí B. Coccimiglio, Camila Ledo, Guillermo H. Docena, Andrea C. Cumino
Parasites & Vectors.2023;[Epub] CrossRef
Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice With Echinococcus granulosus at Different Infection Stages and Their Immunomodulatory Functions
Chunli Shi, Xiaojing Zhou, Wenjuan Yang, Jianwen Wu, Min Bai, Ying Zhang, Wei Zhao, Hui Yang, Atsushi Nagai, Mei Yin, Xiaoping Gao, Shuqin Ding, Jiaqing Zhao
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing
Jing Xiao, Yazhou Zhu, Jianwen Wu, Min Bai, Yunzhuo Xin, Qiang Wang, Jiaqing Zhao
Parasitology Research.2022; 121(7): 1993. CrossRef
Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts
Andrea Maglioco, Facundo A. Agüero, María Pía Valacco, Alejandra Juárez Valdez, Margot Paulino, Alicia G. Fuchs
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis
Xiaola Guo, Shuai Wang, Junmei Zhang, Rui Li, Yong’e Zhang, Zhengrong Wang, Qingming Kong, William C. Cho, Xianghong Ju, Yujuan Shen, Lingqiang Zhang, Haining Fan, Jianping Cao, Yadong Zheng, Alvaro Diaz
PLOS Neglected Tropical Diseases.2022; 16(10): e0010814. CrossRef
Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition
Hynek Mazanec, Peter Koník, Zdenko Gardian, Roman Kuchta
International Journal for Parasitology.2021; 51(5): 327. CrossRef
Identification of Different Extracellular Vesicles in the Hydatid Fluid of Echinococcus granulosus and Immunomodulatory Effects of 110 K EVs on Sheep PBMCs
Jing Yang, Jin'en Wu, Yong Fu, Lujun Yan, Yating Li, Xiaola Guo, Yong'e Zhang, Xiaoqiang Wang, Yujuan Shen, William C. Cho, Yadong Zheng
Frontiers in Immunology.2021;[Epub] CrossRef
Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia
Jie Wang, Jun He, Yucheng Fan, Fangjing Xu, Qian Liu, Ruhua He, Ru Yan
Experimental and Therapeutic Medicine.2021;[Epub] CrossRef
Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages
Li-Qun Wang, Ting-Li Liu, Pan-Hong Liang, Shao-Hua Zhang, Tao-Shan Li, Yan-Ping Li, Guang-Xue Liu, Li Mao, Xue-Nong Luo
Parasites & Vectors.2020;[Epub] CrossRef

8,359 View
173 Download
12 Web of Science
Crossref

Case Report

An Imported Case of Disseminated Echinococcosis in Korea: Dong Hoon Shin, Hae Chan Jo, Jeong-Han Kim, Kang Il Jun, Wan Beom Park, Nam-Joong Kim, Min-Ho Choi, Chang Kyung Kang, Myoung-don Oh; Korean J Parasitol 2019;57(4):429-434.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.429

Abstract
PDF

A complicated case of echinococcosis with multiple organ involvement is reported in a 53-year-old businessman who frequently traveled overseas, including China, Russia, and Kazakhstan from 2001 to 2007. The patient was first diagnosed with a large liver cyst during a screening abdomen ultrasonography in 2011, but he did not follow up on the lesion afterwards. Six years later, dizziness, dysarthria, and cough developed, and cystic lesions were found in the brain, liver and lungs. The clinical course was complicated when the patient went through multiple surgeries and inadequate treatment with a short duration of albendazole without a definite diagnosis. The patient visited our hospital for the first time in August 2018 due to worsening symptoms; he was finally diagnosed with echinococcosis using imaging and serologic criteria. He is now on prolonged albendazole treatment (400 mg twice a day) with gradual clinical and radiological improvement. A high index of suspicion is warranted to early diagnose echinococcosis in a patient with a travel history to endemic areas of echinococcosis.

Citations

Citations to this article as recorded by

Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Delayed Diagnosis of Imported Cystic Echinococcosis and Successful Treatment With Percutaneous Drainage and Albendazole in Korea: A Case Report
Won Jun Choi, Hanna Jin, Hyeon Jae Jo, Chan Mi Lee, Chang Kyung Kang, Pyoeng Gyun Choe, Wan Beom Park, Nam Joong Kim, Min-Ho Choi
Journal of Korean Medical Science.2025;[Epub] CrossRef
Clinical Implications for the Comprehensive Interpretation of Radiologic and Immunodiagnostic Tests in Patients Suspected of Parasitic Hepatic Cyst, a Rare Case in Korea
Jae-Sung Yoo, Min-Kyu Kang, Jung-Gil Park, Hyung-Joo Kim, Joon-Hyuk Choi
Tropical Medicine and Infectious Disease.2023; 8(3): 155. CrossRef
Renal Hydatid With Tuberculosis in a Child: A Rare Coexistence
Sravanthi Vutukuru, Shailesh Solanki, Prema Menon, Nitin Peters, Ram Samujh, Radhika Srinivasan
Advances in Pediatric Surgery.2022; 28(2): 76. CrossRef
Hepatic Hydatid Cyst: A Case Report
Wan Chul Kim, Jae Uk Shin, Su Sin Jin
The Korean Journal of Gastroenterology.2021; 77(1): 35. CrossRef

7,111 View
113 Download
5 Web of Science
Crossref

Original Articles

Neurocysticercosis: Clinical Characteristics and Changes from 26 Years of Experience in an University Hospital in Korea: Hyo-Ju Son, Min Jae Kim, Kyung Hwa Jung, Sungim Choi, Jiwon Jung, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Bong-Kwang Jung, Hyemi Song, Jong-Yil Chai; Korean J Parasitol 2019;57(3):265-271.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.265

Abstract
PDF

The prevalence of human taeniasis has decreased in Korea. The stool egg positive proportion decreased from 1.9% in 1971 to 0% in 2004 in nationwide surveys. The neurocysticercosis (NCC) is also presumed to decrease. However, detailed information regarding the recent status of NCC in Korea is lacking. We retrospectively reviewed NCC cases from 1990 to 2016 at Asan Medical Center, a 2700-bed tertiary referral hospital in Korea. We identified patients based on clinical symptoms, brain imaging, pathology and serological assay. The cases were classified as parenchymal, extraparenchymal, and mixed NCC. Eighty-one patients were included in the analysis. The mean age was 54.5 years, and 79.0% were male. The number of NCC cases was highest from 1995 to 1999, and continuously decreased thereafter. Forty (49.4%) patients had parenchymal NCC, while 25 (30.9%) patients had extraparenchymal NCC, and 16 (19.8%) patients had mixed NCC. The seizure and headache were most common symptom of parenchymal NCC and extraparenchymal NCC respectively. Hydrocephalus was more common in extraparenchymal NCC, and patients with extraparenchymal NCC were more likely to require a ventriculoperitoneal shunt. Cases of NCC are decreasing accordingly with human taeniasis and lesion location was the most important determinant of clinical presentation and outcome of NCC in Korea.

Citations

Citations to this article as recorded by

Navigating a Rare Neurological Conundrum: Quadriparesis in Neurocysticercosis With Hydrocephalus
Mudamanchu Vamsi Krishna, Pubali Biswas, C. A. Jayashankar, V. H. Ganaraja, Amey Joshi
Clinical Case Reports.2025;[Epub] CrossRef
Disseminated Neurocysticercosis With Intraventricular and Cisternal Extension Without Hydrocephalus: A Case Report
Bibek Shrestha, Priyesh Shrestha, Bikram Prasad Gajurel, Grishma Kandel, Laxmi Shah
Clinical Case Reports.2025;[Epub] CrossRef
Intraventricular Neurocysticercosis: Comparative Analysis of Different Localizations. Clinical Course and Treatment: A Systematic Review
Zoran Milenković, Stefan Momčilović, Aleksandra Ignjatović, Aleksandra Aracki-Trenkić, Tanja Džopalić, Nataša Vidović, Zorica Jović, Suzana Tasić-Otašević
Journal of Neurological Surgery Part A: Central European Neurosurgery.2025; 86(05): 467. CrossRef
Neurocysticercosis Presenting as Status Epilepticus
Vaaragie Subramaniam, Jessica Houck DO
Cureus.2025;[Epub] CrossRef
Subarachnoid Neurocysticercosis Manifesting Cognitive Impairment and Gait Disturbance
Minsung Kang, Ho-Won Lee
Journal of the Korean Neurological Association.2025; 43(3): 169. CrossRef
Massive neurocysticercosis in a ten-year-old girl: a case report
Guoguang Xiao, Min Shu
BMC Pediatrics.2024;[Epub] CrossRef
Giant Neurocysticercosis: A Rare Medical Condition
Jorge Zumaeta, Camilo Contreras, Paola Tapia, Diego Morales, Noe Santiago Rea, Jose Valerio
Cureus.2024;[Epub] CrossRef
Knowledge and misconceptions about epilepsy among people with epilepsy and their caregivers attending mental health clinics: A qualitative study in Taenia solium endemic pig‐keeping communities in Tanzania
Charles E Makasi, Andrew M Kilale, Bernard J Ngowi, Yakobo Lema, Victor Katiti, Michael J Mahande, Elizabeth F. Msoka, Dominik Stelzle, Andrea S Winkler, Blandina T. Mmbaga
Epilepsia Open.2023; 8(2): 487. CrossRef
Infectious Causes of Eosinophilic Meningitis in Korean Patients: A Single-Institution Retrospective Chart Review from 2004 to 2018
Sunghee Park, Jiwon Jung, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Min Jae Kim
The Korean Journal of Parasitology.2021; 59(3): 227. CrossRef
Could Differences in Infection Pressure Be Involved in Cysticercosis Heterogeneity?
Pedro T. Hamamoto Filho, Gagandeep Singh, Andrea S. Winkler, Arturo Carpio, Agnès Fleury
Trends in Parasitology.2020; 36(10): 826. CrossRef
Neurocysticercosis Presenting as Homonymous Hemianopia
Hye Jin Lee
Journal of the Korean Ophthalmological Society.2020; 61(9): 1115. CrossRef
Parasitic infections and medical expenses according to Health Insurance Review Assessment claims data in South Korea, 2011–2018
Ju Yeong Kim, Myung-hee Yi, Tai-Soon Yong, Guilherme L. Werneck
PLOS ONE.2019; 14(11): e0225508. CrossRef

7,172 View
152 Download
11 Web of Science
Crossref

Previous Infection with Plasmodium berghei Confers Resistance to Toxoplasma gondii Infection in Mice: Dong-Hun Lee, Ki-Back Chu, Hae-Ji Kang, Su-Hwa Lee, Fu-Shi Quan; Korean J Parasitol 2019;57(2):93-99.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.93

Abstract
PDF

Both Plasmodium spp. and Toxoplasma gondii are important apicomplexan parasites, which infect humans worldwide. Genetic analyses have revealed that 33% of amino acid sequences of inner membrane complex from the malaria parasite Plasmodium berghei is similar to that of Toxoplasma gondii. Inner membrane complex is known to be involved in cell invasion and replication. In this study, we investigated the resistance against T. gondii (ME49) infection induced by previously infected P. berghei (ANKA) in mice. Levels of T. gondii-specific IgG, IgG1, IgG2a, and IgG2b antibody responses, CD4+ and CD8+ T cell populations were found higher in the mice infected with P. berghei (ANKA) and challenged with T. gondii (ME49) compared to that in control mice infected with T. gondii alone (ME49). P. berghei (ANKA) + T. gondii (ME49) group showed significantly reduced the number and size of T. gondii (ME49) cysts in the brains of mice, resulting in lower body weight loss compared to ME49 control group. These results indicate that previous exposure to P. berghei (ANKA) induce resistance to subsequent T. gondii (ME49) infection.

Citations

Citations to this article as recorded by

Functionality of Toxoplasma gondii antibodies in a population of Beninese pregnant women exposed to malaria
Mariama Souffou, Célia Dechavanne, Zaineb Kammoun, Firmine Viwami, Isabelle Gaugué, Naima Beldjoudi, Sébastien Dechavanne, Nawal Sare, André Garcia, Magalie Dambrun, Florence Migot-Nabias
Scientific Reports.2025;[Epub] CrossRef
Neuroprotective and antimalarial effects of Juglans regia leaf extracts in a murine model of cerebral malaria
Afra Alharbi, Shurug Albasyouni, Esam Al-Shaebi, Saleh Al Quraishy, Rewaida Abdel-Gaber
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Protective mucosal and systemic immunity induced by virus-like particles expressing Toxoplasma gondii cyst wall protein
Gi-Deok Eom, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Keon-Woong Yoon, Jie Mao, Su-Hwa Lee, Md Atique Ahmed, Eun-Kyung Moon, Fu-Shi Quan, Paulo Lee Ho
PLOS ONE.2023; 18(4): e0283928. CrossRef
Monocyte-Derived Chicken Macrophages Exposed to Eimeria tenella Sporozoites Display Reduced Susceptibility to Invasion by Toxoplasma gondii Tachyzoite
Runhui Zhang, Wanpeng Zheng, Arwid Daugschies, Berit Bangoura
Microorganisms.2023; 11(8): 1999. CrossRef
Retrospective study of toxoplasmosis prevalence in pregnant women in Benin and its relation with malaria
Magalie Dambrun, Célia Dechavanne, Nicolas Guigue, Valérie Briand, Tristan Candau, Nadine Fievet, Murielle Lohezic, Saraniya Manoharan, Nawal Sare, Firmine Viwami, François Simon, Sandrine Houzé, Florence Migot-Nabias, Gordon Langsley
PLOS ONE.2022; 17(1): e0262018. CrossRef

7,067 View
139 Download
5 Web of Science
Crossref

Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells: Pu Reum Sun, Fei Fei Gao, Hei Gwon Choi, Wei Zhou, Jae-Min Yuk, Jaeyul Kwon, Young-Ha Lee, Guang-Ho Cha; Korean J Parasitol 2019;57(2):83-92.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.83

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.

Citations

Citations to this article as recorded by

Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
Journal of Microbiology.2025; 63(2): e2409001. CrossRef
MjTX-II, a Lys49-PLA2 from Bothrops moojeni snake venom, restricts Toxoplasma gondii infection via ROS and VEGF regulation
Samuel Cota Teixeira, Thales Alves de Melo Fernandes, Guilherme de Souza, Alessandra Monteiro Rosini, Aryani Felixa Fajardo Martínez, Angelica Oliveira Gomes, Rosiane Nascimento Alves, Daiana Silva Lopes, Maria Vitoria da Silva, Emidio Beraldo-Neto, Patrí
Chemico-Biological Interactions.2025; 409: 111417. CrossRef
High-Throughput Repurposing Screen Reveals Compounds with Activity against Toxoplasma gondii Bradyzoites
Taher Uddin, Jing Xia, Yong Fu, Case W. McNamara, Arnab K. Chatterjee, L. David Sibley
ACS Infectious Diseases.2025; 11(3): 600. CrossRef
In Vitro Inhibitory Activity of Corilagin and Punicalagin Against Toxoplasma gondii and Their Mechanism(s) of Action
Nicole T. Green-Ross, Homa Nath Sharma, Audrey Napier, Boakai K. Robertson, Robert L. Green, Daniel A. Abugri
Antibiotics.2025; 14(4): 336. CrossRef
Metabolic changes in Toxoplasma gondii -infected host cells measured by autofluorescence imaging
Gina M. Gallego-López, Emmanuel Contreras Guzman, Danielle E. Desa, Laura J. Knoll, Melissa C. Skala, Anita A. Koshy
mBio.2024;[Epub] CrossRef
BjussuLAAO-II, an l-amino acid oxidase from Bothrops jararacussu snake venom, impairs Toxoplasma gondii infection in human trophoblast cells and villous explants from the third trimester of pregnancy
Thales Alves de Melo Fernandes, Samuel Cota Teixeira, Tássia Rafaela Costa, Alessandra Monteiro Rosini, Guilherme de Souza, Lorena Polloni, Bellisa de Freitas Barbosa, Marcelo José Barbosa Silva, Eloisa Amália Vieira Ferro, Veridiana de Melo Rodrigues Ávi
Microbes and Infection.2023; 25(6): 105123. CrossRef
DNA double-strand breaks in the Toxoplasma gondii-infected cells by the action of reactive oxygen species
Haohan Zhuang, Chaoqun Yao, Xianfeng Zhao, Xueqiu Chen, Yimin Yang, Siyang Huang, Lingtao Pan, Aifang Du, Yi Yang
Parasites & Vectors.2020;[Epub] CrossRef

8,814 View
194 Download
7 Web of Science
Crossref

Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China: Baoping Guo, Zhuangzhi Zhang, Xueting Zheng, Yongzhong Guo, Gang Guo, Li Zhao, Ren Cai, Bingjie Wang, Mei Yang, Xi Shou, Wenbao Zhang, Bin Jia; Korean J Parasitol 2019;57(2):153-159.
Published online April 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.2.153

Abstract
PDF

Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.

Citations

Citations to this article as recorded by

Whole-genomic comparison reveals complex population dynamics and parasitic adaptation of Echinococcus granulosus sensu stricto
Yao-Dong Wu, Zirui Ren, Li Li, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Guo-Dong Dai, Wei-Gang Chen, Wen-Jie Mu, Shuai Wang, Jiandong Li, Qin Yu, Xue-Peng Cai, Xin Jin, Bao-Quan Fu, Daxi Wang, Wan-Zhong Jia, Hong-Bin Yan, Julian Parkhill, James Cotton
mBio.2025;[Epub] CrossRef
First report of Echinococcus granulosus genotype 1 in a wild boar (Sus scrofa) from China
Baoping Guo, Cairen, Li Zhao, Kalibixiati Aimulajiang, Wenqiang Tang, Chuanchuan Wu, Maiweilidan Yimingjiang, Jianyong Wu, Rongsheng Mi, Hao Wen
Parasitology Research.2024;[Epub] CrossRef
Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes – First report from Sialkot District of Pakistan
Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Li Li, Muhammad Saqib, Warda Qamar, Ali Hassan, Muzafar Ghafoor, Siddiq Ur Rahman, Muhammad Umar Zafar Khan, Bao-Quan Fu, Youyu Liu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
Molecular and Biochemical Parasitology.2023; 253: 111542. CrossRef
Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
Pathogens.2023; 12(1): 134. CrossRef
Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
Nigus Abebe Shumuye, Li Li, John Asekhaen Ohiolei, Sayed Ajmal Qurishi, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Sheng-Zhi Gao, Fu-Heng Zhang, Xue-Qi Tian, Wen-Jun Tian, Yong Fu, Xie-Zhong Wang, Yong-Hong Pan, Fang Zhan, Lin-Sheng Zhang, Min
Parasitology Research.2023; 122(5): 1107. CrossRef
Echinococcus granulosus sensu lato Genotypes in Different Hosts Worldwide: A Systematic Review
Carlos Manterola, Armando Totomoch-Serra, Claudio Rojas, Ángela L. Riffo-Campos, Nayely García-Méndez
Acta Parasitologica.2022; 67(1): 161. CrossRef
Genetic diversity of Echinococcus granulosus sensu lato in China: Epidemiological studies and systematic review
Rui‐Qi Hua, Xiao‐Di Du, Xue He, Xiao‐Bin Gu, Yue Xie, Ran He, Jing Xu, Xue‐Rong Peng, Guang‐You Yang
Transboundary and Emerging Diseases.2022;[Epub] CrossRef
Genetic structure and phylogeography of Echinococcus granulosus sensu stricto genotypes G1 and G3 in Pakistan and other regions of the world based on nad5 gene
Naunain Mehmood, Hira Muqaddas, Muhammad Irfan Ullah, Urmas Saarma, Antonio Varcasia
Infection, Genetics and Evolution.2022; 98: 105223. CrossRef
Meta-analysis on the prevalence of bovine hydatid disease in China from 2000 to 2021
Shijie Fan, Hailong Dong, Hongcai Ma, Bingyi Wang, Mujahid Iqbal, Minghao Zou, Ming Qi, Zhipeng Cao
Microbial Pathogenesis.2022; 168: 105586. CrossRef
A CT-based radiomics nomogram for the differentiation of pulmonary cystic echinococcosis from pulmonary abscess
Yan Li, Yaohui Yu, Qian Liu, Haicheng Qi, Shan Li, Juan Xin, Yan Xing
Parasitology Research.2022; 121(12): 3393. CrossRef
Genetic diversity and haplotype analysis of yak and sheep echinococcal cysts isolates from the mitochondrial cox1 gene in parts of Tibet, China
Shijie Fan, Xialing Zhao, Danqulamu, Bin Shi, Wenqiang Tang, Hailong Dong, Chenyang Xia
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Genotyping and phylogenetic analysis of hydatid cysts isolated from livestock in Bushehr province, Iran
Zahra Babaei, Reza Taherkhani, Afshin Barazesh, Marzieh Taherzadeh, Soleiman Khorami, Moradali Fouladvand
Journal of Parasitic Diseases.2021; 45(1): 197. CrossRef
Meta-Analysis of the Prevalence of Echinococcus in Sheep in China From 1983 to 2020
Yang Gao, Wei Wang, Chuang Lyu, Xin-Yu Wei, Yu Chen, Quan Zhao, Zhi-Guang Ran, You-Qing Xia
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process
Junjie Fan, Hongye Wu, Kai Li, Xunuo Liu, Qingqing Tan, Wenqiao Cao, Bo Liang, Bin Ye
The Korean Journal of Parasitology.2020; 58(3): 287. CrossRef
Tacrolimus, a rapamycin target protein inhibitor, exerts anti-cystic echinococcosis effects both in vitro and in vivo
Muzhabaier Muhedier, Jintian Li, Hui Liu, Guizhi Ma, Kuerbannisha Amahong, Renyong Lin, Guodong Lü
Acta Tropica.2020; 212: 105708. CrossRef

8,189 View
125 Download
21 Web of Science
Crossref

Brief Communication

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep: Liu Tianli, Wang Xifeng, Tian Zhenzhong, Wang Lixia, Zhang Xingxing, Qiao Jun, Meng Qingling, Gong Shasha, Chen Ying, Cai Xuepeng; Korean J Parasitol 2019;57(1):61-67.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.61

Abstract
PDF

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

Citations

Citations to this article as recorded by

Rapid and non‐invasive detection of cystic echinococcosis in sheep based on serum fluorescence spectrum combined with machine learning algorithms
Shengke Xu, Wubulitalifu Dawuti, Maierhaba Maimaitiaili, Jingrui Dou, Malike Aizezi, Kalibixiati Aimulajiang, Xiaoyi Lü, Guodong Lü
Journal of Biophotonics.2024;[Epub] CrossRef
Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis
Ana Alice Maia Gonçalves, Anna Julia Ribeiro, Carlos Ananias Aparecido Resende, Carolina Alves Petit Couto, Isadora Braga Gandra, Isabelle Caroline dos Santos Barcelos, Jonatas Oliveira da Silva, Juliana Martins Machado, Kamila Alves Silva, Líria Souza Si
Microbial Cell Factories.2024;[Epub] CrossRef
Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95
Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao
Parasitology.2024; 151(13): 1458. CrossRef
Rapid and accurate screening of cystic echinococcosis in sheep based on serum Fourier‐transform infrared spectroscopy combined with machine learning algorithms
Wubulitalifu Dawuti, Jingrui Dou, Xiangxiang Zheng, Xiaoyi Lü, Hui Zhao, Lingfei Yang, Renyong Lin, Guodong Lü
Journal of Biophotonics.2023;[Epub] CrossRef
Evaluation of a novel Echinococcus granulosus recombinant fusion B-EpC1 antigen for the diagnosis of human cystic echinococcosis using indirect ELISA in comparison with a commercial diagnostic ELISA kit
Enayat Darabi, Elahe Motevaseli, Mehdi Mohebali, Mohammad Bagher Rokni, Mohammad Reza Khorramizadeh, Farzaneh Zahabiun, Soudabeh Heidari, Eshrat Beigom Kia
Experimental Parasitology.2022; 240: 108339. CrossRef
Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line
Andrea Maglioco, Jorge Gentile, Melisa S. Barbery Venturi, Oscar Jensen, Claudia Hernández, María Laura Gertiser, Verónica Poggio, Gabriela Canziani, Alicia Graciela Fuchs
Parasite Immunology.2019;[Epub] CrossRef

7,519 View
130 Download
6 Web of Science
Crossref

Original Article

Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii: Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, H??ng Giang L?, Th? Lam Th?i, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2018;56(5):409-418.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.409

Abstract
PDF

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its expression was observed in both trophozoites and cysts. AcCP was also identified in cultured medium of A. castellanii. Considering to lysosomal localization, secretion or release by trophozoites and continuous expression in trophozoites and cysts, the enzyme could be a multifunctional enzyme that plays important biological functions for nutrition, development and pathogenicity of A. castellanii. These results also imply that AcCP can be a promising target for development of chemotherapeutic drug for Acanthamoeba infections.

Citations

Citations to this article as recorded by

Acanthamoeba castellanii cysteine protease 3 promotes M1 macrophage polarization through the TLR4/NF‑κB pathway
Zhi-xin Wang, Wan-jun Jiao, Mian-jing Wang, Yong Yang, Hai-long Wang, Hong-li Liu
Parasites & Vectors.2025;[Epub] CrossRef
Unraveling the interplay between unicellular parasites and bacterial biofilms: Implications for disease persistence and antibiotic resistance
Eva Zanditenas, Serge Ankri
Virulence.2024;[Epub] CrossRef
Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
Pathogens.2024; 13(2): 142. CrossRef
Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model
Rubén L. Rodríguez-Expósito, Ines Sifaoui, Lizbeth Salazar-Villatoro, Carlos J. Bethencourt-Estrella, José J. Fernández, Ana R. Díaz-Marrero, Robert Sutak, Maritza Omaña-Molina, José E. Piñero, Jacob Lorenzo-Morales
Marine Drugs.2024; 22(9): 423. CrossRef
The gene expression and proteomic profiling of Acanthamoeba isolates
Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta
Experimental Parasitology.2023; 255: 108630. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Induction of Programmed Cell Death in Acanthamoeba culbertsoni by the Repurposed Compound Nitroxoline
Rubén L. Rodríguez-Expósito, Ines Sifaoui, María Reyes-Batlle, Frieder Fuchs, Patrick L. Scheid, José E. Piñero, Robert Sutak, Jacob Lorenzo-Morales
Antioxidants.2023; 12(12): 2081. CrossRef
Paradigms of Protist/Bacteria Symbioses Affecting Human Health: Acanthamoeba species and Trichomonas vaginalis
Fiona L. Henriquez, Ronnie Mooney, Timothy Bandel, Elisa Giammarini, Mohammed Zeroual, Pier Luigi Fiori, Valentina Margarita, Paola Rappelli, Daniele Dessì
Frontiers in Microbiology.2021;[Epub] CrossRef
Trogocytosis in Unicellular Eukaryotes
Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
Cells.2021; 10(11): 2975. CrossRef
Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics
Issam Hasni, Philippe Decloquement, Sandrine Demanèche, Rayane Mouh Mameri, Olivier Abbe, Philippe Colson, Bernard La Scola
Microorganisms.2020; 8(5): 771. CrossRef
Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng
Parasites & Vectors.2020;[Epub] CrossRef
Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins
Abigail Betanzos, Cecilia Bañuelos, Esther Orozco
Genes.2019; 10(8): 618. CrossRef

8,363 View
168 Download
13 Web of Science
Crossref

Brief Communications

Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China: Bin Yan, Xiafei Liu, Junyuan Wu, Shanshan Zhao, Wumei Yuan, Baoju Wang, Hazi Wureli, Changchun Tu, Chuangfu Chen, Yuanzhi wang; Korean J Parasitol 2018;56(4):391-396.
Published online August 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.4.391

Abstract
PDF

Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.

Citations

Citations to this article as recorded by

Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model
Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji
ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY.2025; 13(1): 42. CrossRef
Genetic Variation Within and Between G1 and G3 Genotypes of Echinococcus granulosus sensu stricto from Chile Revealed by Partial DNA Sequencing of rrnS Gene Marker
Victoria Freire, Gittith Sánchez, Flery Fonseca, Alejandro Hidalgo, Alex Vargas, Juan Venegas
Vector-Borne and Zoonotic Diseases.2025; 25(5): 339. CrossRef
Comparative evaluation of mitochondrial gene markers for molecular diagnosis of Echinococcus granulosus sensu lato in clinical samples
Sumeeta Khurana, Reena Yadav, Anupma Dhaka, Abhishek Mewara, Lileshwar Kaman, Ajay Duseja, Naveen Kalra
Diagnostic Microbiology and Infectious Disease.2025; 113(2): 116904. CrossRef
Prevalence and spatial distribution characteristics of human echinococcosis: A county-level modeling study in southern Xinjiang, China
Yue Zhang, Jun Wu, Simayi Adili, Shuo Wang, Haiting Zhang, Guangzhong Shi, Jiangshan Zhao
Heliyon.2024; 10(7): e28812. CrossRef
Epidemiology and genetic diversity of Echinococcus granulosus sensu stricto in the East Tianshan Mountains, Xinjiang, China
Wulijiang Kamali, Si-Yun Wang, Wei-Dong Luo, Shuai Liu, Li Zhao, Xing-Yu Pan, Bing-Jie Wang, Yong-Hui Mu, Tuoliehuojia Jiawuti, Kadierding Aierken, Zhuang-Zhi Zhang, Wan-Li Ban
Parasitology Research.2024;[Epub] CrossRef
Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
Pathogens.2023; 12(1): 134. CrossRef
Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
Nigus Abebe Shumuye, Li Li, John Asekhaen Ohiolei, Sayed Ajmal Qurishi, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Sheng-Zhi Gao, Fu-Heng Zhang, Xue-Qi Tian, Wen-Jun Tian, Yong Fu, Xie-Zhong Wang, Yong-Hong Pan, Fang Zhan, Lin-Sheng Zhang, Min
Parasitology Research.2023; 122(5): 1107. CrossRef
Molecular characterization of cattle and sheep isolates of Echinococcus granulosus from Elazig province in Turkey and expression analysis of the non-coding RNAs, egr-miR-7, egr-miR-71 and egr-miR-96
Bunyamin Irehan, Figen Celik, Ergun Koroglu, Ahmet Tektemur, Sami Simsek
Experimental Parasitology.2023; 251: 108551. CrossRef
Echinococcus granulosus sensu lato Genotypes in Different Hosts Worldwide: A Systematic Review
Carlos Manterola, Armando Totomoch-Serra, Claudio Rojas, Ángela L. Riffo-Campos, Nayely García-Méndez
Acta Parasitologica.2022; 67(1): 161. CrossRef
Human Cystic Echinococcosis in Lebanon: A Retrospective Study and Molecular Epidemiology
Gaelle Joanny, Maria Grazia Cappai, Francesca Nonnis, Claudia Tamponi, Giorgia Dessì, Naunain Mehmood, Julien Dahdah, Chadi Hosri, Antonio Scala, Antonio Varcasia
Acta Parasitologica.2022; 67(1): 186. CrossRef
Meta-analysis of the prevalence of bovine cystic echinococcosis in China during decade
Xin-Bo Yang, Xiang-Zhu Meng, Yan Zhao, Jin-Ping Zhao, Chao Chen, Ya Qin, Yuan Zhang, Quan Zhao
Research in Veterinary Science.2022; 152: 465. CrossRef
Food-borne zoonotic echinococcosis: A review with special focus on epidemiology
Mughees Aizaz Alvi, Abdullah F. Alsayeqh
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Theileria, Hepatozoon and Taenia infection in great gerbils (Rhombomys opimus) in northwestern China
Na Ji, Xueling Chen, Gang Liu, Shanshan Zhao, Wenbo Tan, Guangyuan Liu, Jiangguo Zhang, Yuanzhi Wang
International Journal for Parasitology: Parasites and Wildlife.2021; 15: 79. CrossRef
Advances in research on echinococcoses epidemiology in China
Mei-Hua Fu, Xu Wang, Shuai Han, Ya-Yi Guan, Robert Bergquist, Wei-Ping Wu
Acta Tropica.2021; 219: 105921. CrossRef
Multiple haplotypes of Echinococcus granulosus sensu stricto in single naturally infected intermediate hosts
Christian Hidalgo, Caroll Stoore, Ismael Pereira, Rodolfo Paredes, Cristian A. Alvarez Rojas
Parasitology Research.2020; 119(2): 763. CrossRef
miRNAs and lncRNAs in Echinococcus and Echinococcosis
Zhi He, Taiming Yan, Ya Yuan, Deying Yang, Guangyou Yang
International Journal of Molecular Sciences.2020; 21(3): 730. CrossRef
Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)
N.M. AL-Mutairi, H.A. Taha, A.H. Nigm
Journal of Helminthology.2020;[Epub] CrossRef

9,014 View
144 Download
19 Web of Science
Crossref

A Retrospective Analysis on the Cystic Echinococcosis Cases Occured in Northeastern Punjab Province, Pakistan: Aisha Khan, Sarmad Zahoor, Haroon Ahmed, Uzma Malik, Rabia Amin Butt, Muhammad Sultanul Muzam, Seyma Gunyakti Kilinc, Noorulain Noor, Saroosh Zahoor, Muhammad Sohail Afzal, Hamza Mansur, Shumaila Irum, Sami Simsek; Korean J Parasitol 2018;56(4):385-390.
Published online August 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.4.385

Abstract
PDF

A retrospective study was performed to report the case occurrence of cystic echinococcosis (CE) in three hospitals of north-eastern region in Punjab Province, Pakistan. We reviewed retrospectively the clinical records of patients in 4 hospitals which were diagnosed with CE during 2012-2017. A total of 198 cases, 82 (41.4%) male and 116 (58.6%) females were detected as CE. The most Highest incidence was revealed in.... 21-30 years-old group (24.2%) followed by 41-50 (22.7%), 31-40 (16.2%), 11-20 (13.6%), 51-60 (8.1%), below 10 (5.5%), over 71 (5.1%) and 61-70 year-old group (4.5%). CE was detected in various organs of infected individuals. However, most of CE cases were detected in the liver (47.4%) and lung (18.6%). The present study indicated that CE is more or less prevalent in surveyed areas and one of the most important public health problems in Punjab Province, Pakistan.

Citations

Citations to this article as recorded by

Prevalence and molecular characterization of cystic echinococcosis in livestock in the Hazara Division, Pakistan
Safia Arbab, Hanif Ullah, Inam Ul Hassan, Suleman, Weiwei Wang, Abdul Qadeer, Jiya Zhang
Frontiers in Veterinary Science.2025;[Epub] CrossRef
A Retrospective Cohort Study on Human Cystic Echinococcosis in Khyber Pakhtunkhwa Province (Pakistan) Based on 16 Years of Hospital Discharge Records
Huma Khan, Adriano Casulli, Majid Fasihi Harandi, Muhammad Sohail Afzal, Muhammad Arif Nadeem Saqib, Haroon Ahmed
Pathogens.2022; 11(2): 194. CrossRef
Design of highly sensitive nano-biosensor for diagnosis of hydatid cyst based on gold nanoparticles
Fariba Jafari, Amir Hossein Maghsood, Mohammad Fallah, Ahmad Jalilvand, Mohammad Matini, Bahram Amini
Photodiagnosis and Photodynamic Therapy.2022; 38: 102786. CrossRef
Seropozitifity of Anti-Echinococcus granulosus in Patients with of Clinical Prediagnosis Cystic Echinococcosis at Kafkas University Health Research and Application Hospital
Mükremin Özkan Arslan, Neriman Mor, Hilal Bedir
Turkish Journal of Parasitology.2022; 46(2): 129. CrossRef
Investigation of Echinococcus multilocularis in foxes and dogs in Pakistan by detection of copro-DNA
Aisha Khan, Gérald Umhang, Zaib Ullah, Franck Boué, Vanessa Bastid, Ikram Ullah, Sajid Mahmood, Muhammad Sohail Afzal, Haroon Ahmed
Parasitology Research.2021; 120(2): 731. CrossRef
Case report of an unexpected inflammatory myofibroblastic tumor
Vishal Farid Raza, Dawood Arshad, Sajeel Ahmad, Khalid Javeed Khan
Annals of Medicine & Surgery.2021;[Epub] CrossRef
A review of the diagnosis and management of liver hydatid cyst
Ester Ferrer-Inaebnit, Francesc Xavier Molina-Romero, Juan José Segura-Sampedro, Xavier González-Argenté, José Miguel Morón Canis
Revista Española de Enfermedades Digestivas.2021;[Epub] CrossRef
Demographics of cystic echinococcosis patients treated surgically in Lahore, Pakistan: A single centre study from 2007 – 2018
Q. Rasib, A. Khan, H. Ahmed, S. Nizamuddin, F. Asif, M. S. Afzal, S. Simsek, F. Khurshid, S. Irum, N. Hussain, S. Riaz, S. S. Khan, C. M. Budke
Helminthologia.2021; 58(2): 162. CrossRef
Echinococcoses in Iran, Turkey, and Pakistan: Old Diseases in the New Millennium
Mehdi Borhani, Saeid Fathi, Enayat Darabi, Fatemeh Jalousian, Sami Simsek, Haroon Ahmed, Harun Kaya Kesik, Seyed Hossein Hosseini, Thomas Romig, Majid Fasihi Harandi, Iraj Mobedi
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Clinical and pathogenetic aspects of the complications after surgical treatment of hepatic echinococcosis
F. G. Nazyrov, A. Kh. Babadjanov, F. R. Yakubov
Annaly khirurgicheskoy gepatologii = Annals of HPB Surgery.2021; 26(4): 51. CrossRef
Detection of Anti-Echinococcus granulosus Antibodies in Humans: An Update from Pakistan
Huma Khan, Haroon Ahmed, Muhammad Sohail Afzal, Usman Ayub Awan, Muhammad Khurram, Sami Simsek, Jianping Cao
Pathogens.2021; 11(1): 29. CrossRef
Cystic echinococcosis: a 10-year experience from a middle-income country
Ayesha Butt, Javaid Ahmed Khan
Tropical Doctor.2020; 50(2): 117. CrossRef
First report of Echinococcus canadensis (G6/G7) by sequence analysis from the Khyber Pakhtunkhwa province of Pakistan
Aisha Khan, Haroon Ahmed, Huma Khan, Sami Simsek, Seyma Gunyakti Kilinc, Harun Kaya Kesik, Guan Yayi, Figen Celik, Muhammad Sohail Afzal, Christine M. Budke
Acta Tropica.2020; 209: 105559. CrossRef
Human cystic echinococcosis in southwest Iran: a 15-year retrospective epidemiological study of hospitalized cases
Reza Shahriarirad, Amirhossein Erfani, Mehrdad Eskandarisani, Mohammad Rastegarian, Hajar Taghizadeh, Bahador Sarkari
Tropical Medicine and Health.2020;[Epub] CrossRef
Cystic Echinococcosis in Pakistan: A Review of Reported Cases, Diagnosis, and Management
Aisha Khan, Haroon Ahmed, Huma Khan, Sadia Saleem, Sami Simsek, Enrico Brunetti, Muhammad Sohail Afzal, Tommaso Manciulli, Christine M. Budke
Acta Tropica.2020; 212: 105709. CrossRef
Management Outcome in Simple and Complex Hydatid Cysts of Lung
Pratikshya Thapaliya, Tanveer Ahmad, Ambreen Abid, Nazish Sikander, Misauq Mazcuri, Nadir Ali
Cureus.2020;[Epub] CrossRef
Retrospective Study of Cystic Echinococcosis (CE) Based on Hospital Record from Five Major Metropolitan Cities of Pakistan
Hira Muqaddas, Muhammad Arshad, Haroon Ahmed, Naunain Mehmood, Aisha Khan, Sami Simsek
Acta Parasitologica.2019; 64(4): 866. CrossRef

8,481 View
157 Download
16 Web of Science
Crossref

Original Articles

Pyruvate Protects Giardia Trophozoites from Cysteine-Ascorbate Deprived Medium Induced Cytotoxicity: Dibyendu Raj, Punam Chowdhury, Rituparna Sarkar, Yumiko Saito-Nakano, Keinosuke Okamoto, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly; Korean J Parasitol 2018;56(1):1-9.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.1

Giardia lamblia, an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia. In the present study, we have examined the effects of extracellular addition of pyruvate, during oxidative stress generated from cysteine-ascorbate deprivation in culture media on DNA damage in Giardia. The intracellular pyruvate concentrations at several time points were measured in the trophozoites during stress. Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. The exogenous addition of a physiologically relevant concentration of pyruvate to trophozoites suspension was shown to attenuate the rate of ROS generation. We have demonstrated that Giardia protects itself from destructive consequences of ROS by maintaining the intracellular pyruvate concentration. Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair.

Citations

Citations to this article as recorded by

A myeloid leukemia factor homolog is involved in tolerance to stresses and stress-induced protein metabolism in Giardia lamblia
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Biology Direct.2023;[Epub] CrossRef
Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate
Yana Shaulov, Chikako Shimokawa, Meirav Trebicz-Geffen, Shruti Nagaraja, Karen Methling, Michael Lalk, Lea Weiss-Cerem, Ayelet T. Lamm, Hajime Hisaeda, Serge Ankri, William A. Petri
PLOS Pathogens.2018; 14(10): e1007295. CrossRef

9,089 View
204 Download
2 Web of Science
Crossref

Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae: Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Suk-Yul Jung, Ho-Joon Shin; Korean J Parasitol 2017;55(3):233-238.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.233

Abstract
PDF

Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp., a defined, slightly modified liquid media is used. In this study, in order to generate pure N. fowleri cysts, a liquid encystment medium (buffer 1) modified from Page’s amoeba saline was applied for encystation of N. fowleri. N. fowleri cysts were well induced after 24 hr with the above defined liquid encystment medium (buffer 1). This was confirmed by observation of a high expression of differential mRNA of nfa1 and actin genes in trophozoites. Thus, this liquid medium can replace the earlier non-nutrient agar medium for obtaining pure N. fowleri cysts. In addition, for cyst formation of Acanthamoeba spp., buffer 2 (adjusted to pH 9.0) was the more efficient medium. To summarize, these liquid encystment media may be useful for further studies which require axenic and pure amoebic cysts.

Citations

Citations to this article as recorded by

Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.
Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Alok K. Paul, Sonia M. Rodrigues Oliveira, Maria L. Pereira, Tajudeen O. Jimoh, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Ajoy K. Verma, Veeranoot Nissapatorn
Veterinary World.2025; : 110. CrossRef
Exploring the potential role of soil protists in predicting banana health
Xiangyu Ren, Chen Liu, Yang Yue, Shuo Sun, Lang Zhao, Chengyuan Tao, Beibei Wang, Wu Xiong, Zongzhuan Shen, Rong Li, Qirong Shen
Microbiological Research.2025; 295: 128109. CrossRef
Cloning of nf-profilin and intercellular interaction with nf-actin in Naegleria fowleri cysts
Hae-Jin Sohn, A-Jeong Ham, A-Young Park, Jeong-Heon Lee, Sun Park, Ho-Joon Shin, Jong-Hyun Kim
Scientific Reports.2025;[Epub] CrossRef
Ouabain, ATPase inhibitor, potentially enhances the effect of polyhexamethylene biguanide on Acanthamoeba castellanii
Kuang-Yi Shih, Yao-Tsung Chang, Yu-Jen Wang, Jian-Ming Huang
International Journal for Parasitology: Drugs and Drug Resistance.2024; 25: 100550. CrossRef
Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1)
Jong-Hyun Kim, Hae-Jin Sohn, Ho-Joon Shin, Stacy E. Walz, Suk-Yul Jung
Parasites, Hosts and Diseases.2024; 62(4): 385. CrossRef
De Novo Transcriptome Profiling of Naegleria fowleri Trophozoites and Cysts via RNA Sequencing
Hae-Jin Sohn, Jong-Hyun Kim, Kyongmin Kim, Sun Park, Ho-Joon Shin
Pathogens.2023; 12(2): 174. CrossRef
An International External Quality Assessment Scheme to Assess the Diagnostic Performance of Polymerase Chain Reaction Detection of Acanthamoeba Keratitis
Maarten J. Sarink, Rob Koelewijn, Foekje Stelma, Titia Kortbeek, Lisette van Lieshout, Pieter W. Smit, Aloysius G. M. Tielens, Jaap J. van Hellemond
Cornea.2023; 42(8): 1027. CrossRef
Development of a Machine Learning-Based Cysticidal Assay and Identification of an Amebicidal and Cysticidal Marine Microbial Metabolite against Acanthamoeba
Brian Shing, Mina Balen, William Fenical, Anjan Debnath, Wei-Hua Chen
Microbiology Spectrum.2022;[Epub] CrossRef
Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
Rachasak Boonhok, Suthinee Sangkanu, Suganya Phumjan, Ramita Jongboonjua, Nawarat Sangnopparat, Pattamaporn Kwankaew, Aman Tedasen, Chooi Ling Lim, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Alok
PeerJ.2022; 10: e13657. CrossRef
Novel Antiamoebic Tyrocidine-Derived Peptide against Brain-Eating Amoebae
Noor Akbar, Wendy E. Kaman, Maarten Sarink, Kamran Nazmi, Floris J. Bikker, Naveed Ahmed Khan, Ruqaiyyah Siddiqui
ACS Omega.2022; 7(33): 28797. CrossRef
Combinatorial treatment with β-glucanase enzyme and chlorhexidine induces cysticidal effects in Acanthamoeba cyst
Nurhidayana Mohd Rased, Syed Ahmad Tajudin Tuan Johari, Hazlina Ahamad Zakeri, Nyuk Ling Ma, Siti Aisyah Razali, Fatimah Hashim
Parasitology Research.2022; 121(11): 3105. CrossRef
The Acanthamoeba SBDS, a cytoskeleton-associated gene, is highly expressed during phagocytosis and encystation
Yu-Jen Wang, Wei-Chen Lin, Ming-Shan He
Journal of Microbiology, Immunology and Infection.2021; 54(3): 482. CrossRef
RETRACTED ARTICLE: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
Heekyoung Kang, Hae-Jin Sohn, A-Young Park, A-Jeong Ham, Jeong-Heon Lee, Young-Hwan Oh, Yong-Joon Chwae, Kyongmin Kim, Sun Park, Hongseok Yang, Suk-Yul Jung, Jong-Hyun Kim, Ho-Joon Shin
Scientific Reports.2021;[Epub] CrossRef
A Novel Cysteine Protease Inhibitor of Naegleria fowleri That Is Specifically Expressed during Encystation and at Mature Cysts
Hương Giang Lê, A-Jeong Ham, Jung-Mi Kang, Tuấn Cường Võ, Haung Naw, Hae-Jin Sohn, Ho-Joon Shin, Byoung-Kuk Na
Pathogens.2021; 10(4): 388. CrossRef
Inhibitory Effect of Host Ocular Microenvironmental Factors on Chlorhexidine Digluconate Activity
Chun-Hsien Chen, Yu-Jen Wang, Jian-Ming Huang, Fu-Chin Huang, Wei-Chen Lin
Antimicrobial Agents and Chemotherapy.2021;[Epub] CrossRef
Primary Amoebic Meningoencephalitis by Naegleria fowleri: Pathogenesis and Treatments
Andrea Güémez, Elisa García
Biomolecules.2021; 11(9): 1320. CrossRef
Evaluation of Amebicidal and Cysticidal Activities of Antifungal Drug Isavuconazonium Sulfate against Acanthamoeba T4 Strains
Brian Shing, Mina Balen, Anjan Debnath
Pharmaceuticals.2021; 14(12): 1294. CrossRef
Characterisation of the β-lactam resistance enzyme in Acanthamoeba castellanii
Chun-Hsien Chen, Chao-Li Huang, Ming-Shan He, Fu-Chin Huang, Wei-Chen Lin
International Journal of Antimicrobial Agents.2020; 55(2): 105823. CrossRef
Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea
Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin
Scientific Reports.2020;[Epub] CrossRef
Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro
Thị Lam Thái, Jung-Mi Kang, Hương Giang Lê, Jinyoung Lee, Won Gi Yoo, Ho-Joon Shin, Woon-Mok Sohn, Byoung-Kuk Na
Parasites & Vectors.2020;[Epub] CrossRef
The Antifungal Drug Isavuconazole Is both Amebicidal and Cysticidal against Acanthamoeba castellanii
Brian Shing, Seema Singh, Larissa M. Podust, James H. McKerrow, Anjan Debnath
Antimicrobial Agents and Chemotherapy.2020;[Epub] CrossRef
Effects of Acanthamoeba castellanii on the dissolved oxygen and the microbial community under the experimental aquatic model
Chih-Ming Tsai, Jenn-Wei Chen, Wei-Chen Lin
Experimental Parasitology.2020; 218: 107985. CrossRef
Fate of internalized Campylobacter jejuni and Mycobacterium avium from encysted and excysted Acanthamoeba polyphaga
Rasha Maal-Bared, Brent Dixon, Diana Axelsson-Olsson
Experimental Parasitology.2019; 199: 104. CrossRef
Cellular characterization of actin gene concerned with contact‐dependent mechanisms in Naegleria fowleri
Hae‐Jin Sohn, Kyoung‐Ju Song, Heekyoung Kang, A‐Jeong Ham, Jae‐Ho Lee, Yong‐Joon Chwae, Kyongmin Kim, Sun Park, Jong‐Hyun Kim, Ho‐Joon Shin
Parasite Immunology.2019;[Epub] CrossRef
Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts
Hae-Jin Sohn, Ga-Eun Seo, Jae-Ho Lee, A-Jeong Ham, Young-Hwan Oh, Heekyoung Kang, Ho-Joon Shin
The Korean Journal of Parasitology.2019; 57(3): 217. CrossRef
Production and characterization of monoclonal antibodies against cathepsin B and cathepsin B-Like proteins of Naegleria fowleri
Gi-Sang Seong, Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Ho-Joon Shin
Experimental Parasitology.2017; 183: 171. CrossRef

9,738 View
240 Download
27 Web of Science
Crossref

DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation: Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong; Korean J Parasitol 2017;55(2):115-120.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.115

Abstract
PDF

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.

Citations

Citations to this article as recorded by

DNA methylation modification: Dawn of research on cornea-related diseases
Quanhao Pan, Xiaoning Ge, Di Wang, Yuxi He
Life Sciences.2025; 376: 123757. CrossRef
Proteases of Acanthamoeba
Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani
Parasitology Research.2024;[Epub] CrossRef
Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease
Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui
Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Coevolution of the CDCA7-HELLS ICF-related nucleosome remodeling complex and DNA methyltransferases
Hironori Funabiki, Isabel E Wassing, Qingyuan Jia, Ji-Dung Luo, Thomas Carroll
eLife.2023;[Epub] CrossRef
Coevolution of the CDCA7-HELLS ICF-related nucleosome remodeling complex and DNA methyltransferases
Hironori Funabiki, Isabel E Wassing, Qingyuan Jia, Ji-Dung Luo, Thomas Carroll
eLife.2023;[Epub] CrossRef
Antiproliferation and Antiencystation Effect of Class II Histone Deacetylase Inhibitors on Acanthamoeba castellanii
Ki-Back Chu, Hae-Ahm Lee, Marc Pflieger, Fabian Fischer, Yodita Asfaha, Leandro A. Alves Avelar, Alexander Skerhut, Matthias U. Kassack, Finn K Hansen, Andrea Schöler, Thomas Kurz, Min-Jeong Kim, Eun-Kyung Moon, Fu-Shi Quan
ACS Infectious Diseases.2022; 8(2): 271. CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Aspergillus niger trehalase enzyme induced morphological and protein alterations on Acanthamoeba cyst and molecular docking studies
H. Fatimah, R. Siti Aisyah, N. L. Ma, Nurhidayana M. Rased, Nor F. A. C. Mohamad, F. Nur Syakinah Nafisa, A. Azila, Hazlina A. Zakeri
Journal of Parasitic Diseases.2021; 45(2): 459. CrossRef
Epigenetics as Driver of Adaptation and Diversification in Microbial Eukaryotes
Agnes K. M. Weiner, Laura A. Katz
Frontiers in Genetics.2021;[Epub] CrossRef
Infectious Keratitis: An Update on Role of Epigenetics
Sudhir Verma, Aastha Singh, Akhil Varshney, R. Arun Chandru, Manisha Acharya, Jyoti Rajput, Virender Singh Sangwan, Amit K. Tiwari, Tuhin Bhowmick, Anil Tiwari
Frontiers in Immunology.2021;[Epub] CrossRef
aCLS cancers: Genomic and epigenetic changes transform the cell of origin of cancer into a tumorigenic pathogen of unicellular organization and lifestyle
Vladimir F. Niculescu
Gene.2020; 726: 144174. CrossRef
New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy
Fernando Pérez-Cota, Richard J. Smith, Hany M. Elsheikha, Matt Clark
Biomedical Optics Express.2019; 10(5): 2399. CrossRef
Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts
Hae-Jin Sohn, Ga-Eun Seo, Jae-Ho Lee, A-Jeong Ham, Young-Hwan Oh, Heekyoung Kang, Ho-Joon Shin
The Korean Journal of Parasitology.2019; 57(3): 217. CrossRef

7,975 View
171 Download
11 Web of Science
Crossref

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii: Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan; Korean J Parasitol 2017;55(2):109-114.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.109

Abstract
PDF

Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the encystation process has to be regulated in order to be induced or inhibited. However, the regulation mechanism of these genes is yet unknown. In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFPAcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulation of encystation of A. castellanii. The target gene of AcPRMT1 regulation and the detailed mechanisms need to be investigated by further studies.

Citations

Citations to this article as recorded by

PRMT5 Inhibitor EPZ015666 Decreases the Viability and Encystment of Entamoeba invadens
Rigoberto Ortiz-Hernández, Elmer Joel Millán-Casarrubias, Jeni Bolaños, Susana Munguía-Robledo, Carlos Vázquez-Calzada, Elisa Azuara-Licéaga, Jesús Valdés, Mario Alberto Rodríguez
Molecules.2024; 30(1): 62. CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Drug Discovery against Acanthamoeba Infections: Present Knowledge and Unmet Needs
Hany M. Elsheikha, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Pathogens.2020; 9(5): 405. CrossRef
Free-living amoebae and squatters in the wild: ecological and molecular features
Ascel Samba-Louaka, Vincent Delafont, Marie-Hélène Rodier, Estelle Cateau, Yann Héchard
FEMS Microbiology Reviews.2019; 43(4): 415. CrossRef

9,987 View
223 Download
4 Web of Science
Crossref

Case Report

A Case of Cerebral Cysticercosis in Thailand: Samasuk Thammachantha, Ratana Kunnatiranont, Pongwat Polpong; Korean J Parasitol 2016;54(6):793-795.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.793

Abstract
PDF

Cysticercosis and sparganosis are not uncommon parasitic infections in the developing world. Central nervous system infection by both cestodes can present with neurological signs and symptoms, such as seizure and mass effect, including brain hernia. Early detection and accurate diagnosis can prevent a fatal outcome. Histological examinations of brain tissues can confirm the diagnosis of cerebral cysticercosis, which differs from sparganosis by the presence of a cavitated body. We report here a case of cerebral cysticercosis which has the similar clinical and imaging findings as sparganosis.

Citations

Citations to this article as recorded by

Neurosurgical Approach to Neurocysticercosis in Adults: A Comprehensive Systematic Review of Clinical and Imaging Insights
Maxon V. Knott, Chandler N. Berke, Khushi Hemendra Shah, Muhammet Enes Gurses, Malek Bashti, Victor M. Lu, Michael E. Ivan, Jose G. Castro, Ricardo J. Komotar, Ashish H. Shah
Neurosurgery.2025; 97(2): 281. CrossRef
Spinal Sparganosis Coexisting with Acquired Arteriovenous Fistula of the Filum Terminale
Prasert Iampreechakul, Wuttipong Tirakotai, Punjama Lertbutsayanukul, Sirirat Khunvutthidee, Samasuk Thammachantha, Somkiet Siriwimonmas
World Neurosurgery.2020; 136: 341. CrossRef

11,108 View
163 Download
Crossref

Original Articles

Factors Associated with Echinococcosis-Induced Perioperative Anaphylactic Shock: Jianrong Ye, Qin Zhang, Yan Xuan, Siyu Chen, Long Ma, Yongqiang Zhang, Hong Zheng; Korean J Parasitol 2016;54(6):769-775.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.769

This retrospective case-control study explored the factors associated with anaphylactic shock during surgery for cystic echinococcosis (CE) at the First Affiliated Hospital of Xinjiang Medical University between October 2008 and September 2013. Patients who suffered from anaphylactic shock (n=16) were age-matched 3:1 to patients who did not (n=43). Multivariate analysis suggested that IL-4 levels (odds ratio=1.096; 95% confidence interval=1.015-1.185; P=0.02) and cyst size (odds ratio=3.028, 95% confidence interval=1.259-7.283, P=0.013) were independently associated with CE-induced perioperative anaphylactic shock. Using the receiver operating characteristic (ROC) curves and a cut-off value of 415.7 ng/ml, IL-4 showed an area under the ROC (AUC) of 0.926, sensitivity of 75.0%, and specificity of 97.7%. Using a cut-off value of 7.8 cm, cyst size showed an AUC of 0.828, sensitivity of 81.3%, and specificity of 76.7%. In conclusion, results suggest that levels of IL-4 and cyst size were independently associated with echinococcosis-induced perioperative anaphylactic shock. These results could help identifying patients with echinococcosis at risk of anaphylactic shock in whom appropriate prophylaxis could be undertaken.

Citations

Citations to this article as recorded by

Impact of Echinococcus granulosus Cyst Fluid on the Phenotypic Dynamics and Degranulation of RBL-2H3 Mast Cells
Zhen Yue, Jing-Ru Zhou, Chun-Sheng Wang, Xue-Li Pu, Guligeiya Parehati, Li-Wei Cao, Batesurong Bayina, Jian-Rong Ye
Journal of Inflammation Research.2025; Volume 18: 9837. CrossRef
Predictive Factors of Pulmonary Hydatid Complications in Children
Nahla Kechiche, Arije Zouaoui, Asma Ben Cheikh, Rachida Lamiri, Amine Ksia, Mongi Mekki, Lassaad Sahnoun
Journal of Pediatric Surgery.2024; 59(12): 161688. CrossRef
Response to Stockdale and Savic, regarding ‘Peri‐operative refractory anaphylactic shock during hepatic hydatid cyst surgery’
P. Gundappa, D. Prakash, N. Palankar, S. Karanth
Anaesthesia Reports.2023;[Epub] CrossRef
Popliteal Echinococcosis: A Long Journey from the Liver
Ergin Erginöz, Sefa Ergün, Emre Tunç, Salih Pekmezci
Acta Parasitologica.2023; 68(2): 463. CrossRef
The regulatory role of differential microRNA expressions on cellular inflammatory factors IL‐6 and IL‐10 in Echinococcus granulosus‐induced anaphylaxis
Chun‐sheng Wang, Tao Yu, Xilizhati Kulaixi, Jing‐ru Zhou, Xianyidan Abulajiang, Jia‐ling Wang, Si‐jia Wang, Jian‐rong Ye
Immunity, Inflammation and Disease.2023;[Epub] CrossRef
The role of memory T cells in Echinococcus granulosus‐induced sensitization
Jing‐Ru Zhou, Xiao‐Xuan Du, Xianyidan Abulajiang, Wuer Geli, Xue‐Li Pu, Subi Tailaiti, Jia‐Ying Lin, Yu‐Qian Li, Jian‐Rong Ye
Immunity, Inflammation and Disease.2023;[Epub] CrossRef
Peri‐operative refractory anaphylactic shock during hepatic hydatid cyst surgery
P. Gundappa, D. Prakash, N. Palankar, S. Karanth
Anaesthesia Reports.2022;[Epub] CrossRef
Perioperative Anaphylaxie – alte Zöpfe und Neues zu den Auslösern
Christiane Bayerl
Laryngo-Rhino-Otologie.2022; 101(11): 882. CrossRef
Perioperative Anaphylaxie – alte Zöpfe und Neues zu den Auslösern
C. Bayerl
Aktuelle Dermatologie.2021; 47(04): 155. CrossRef
Construction of ceRNA regulatory network in mice with Echinococcosis-induced allergic reactions
Xiaodong Yu, Yali Yasen, Chunsheng Wang, Meng Li, Zhiyuan Fang, Jialing Wang, Jianrong Ye
Acta Tropica.2021; 224: 106120. CrossRef
Isolated renal hydatid disease: varied presentations, treatments, dilemmas, and the way ahead: case report series
Ankit Misra, Swarnendu Mandal, Manoj Das, Pritinanda Mishra, Suvradeep Mitra, Prasant Nayak
African Journal of Urology.2021;[Epub] CrossRef
Endoscopic excision of hydatid cyst: A case of delayed anaphylaxis in postoperative period - “The risk is not over until it is over”
Aakriti Gupta, Neerja Bhardwaj, Soumya Sarkar, Ravi P. Kanojia
Journal of Clinical Anesthesia.2019; 52: 63. CrossRef
Should Pediatricians Be Aware of Cystic Echinococcosis? A Literature Review
Chiara Tersigni, Elisabetta Venturini, Carlotta Montagnani, Leila Bianchi, Elena Chiappini, Maurizio de Martino, Luisa Galli
Journal of Pediatric Gastroenterology and Nutrition.2019; 68(2): 161. CrossRef
Recurrent multiple-organ involvement of disseminated alveolar echinococcosis in 3 patients
Leilei Xu, Sikandaer Siyiti, Xinghua Song, Hao Wen, Liwen Ding, Akbar Yunus, Zheng Tian, Chong Wang, Jiangtao Chen
Medicine.2017; 96(42): e7632. CrossRef

9,749 View
180 Download
18 Web of Science
Crossref

Molecular Cloning and Characterization of a P38-Like Mitogen-Activated Protein Kinase from Echinococcus granulosus: Guodong L?, Jing Li, Chuanshan Zhang, Liang Li, Xiaojuan Bi, Chaowang Li, Jinliang Fan, Xiaomei Lu, Dominique A. Vuitton, Hao Wen, Renyong Lin; Korean J Parasitol 2016;54(6):759-769.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.759

Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus granulosus. We identified a 1,107 bp cDNA encoding a 368 amino acid MAPK protein (Egp38) in E. granulosus. Egp38 exhibits 2 distinguishing features of p38-like kinases: a highly conserved T-X-Y motif and an activation loop segment. Structural homology modeling indicated a conserved structure among Egp38, EmMPK2, and H. sapiens p38α, implying a common binding mechanism for the ligand domain and downstream signal transduction processing similar to that described for p38α. Egp38 and its phosphorylated form are expressed in the E. granulosus larval stages vesicle and protoscolices during intermediate host infection of an intermediate host. Treatment of in vitro cultivated protoscolices with the p38-MAPK inhibitor ML3403 effectively suppressed Egp38 activity and led to significant protoscolices death within 5 days. Treatment of in vitro-cultivated protoscolices with TGF-β1 effectively induced Egp38 phosphorylation. In summary, the MAPK, Egp38, was identified in E. granulosus, as an anti-CE drug target and participates in the interplay between the host and E. granulosus via human TGF-β1.

Citations

Citations to this article as recorded by

Echinococcus granulosus promotes MAPK pathway-mediated osteoclast differentiation by inhibiting Nrf2 in osseous echinococcosis
Yaqing Liu, Jing Li, Zhendong Zhang, Qi Li, Yanhu Tian, Sibo Wang, Chenhui Shi, Haohao Sun
Veterinary Research.2025;[Epub] CrossRef
In vitro Scolicidal Efficacy of 5-Fluorouracil and Radiation Against Protoscoleces of Echinococcus granulosus Sensu Lato
Pengfei Lu, Jun Li, Rui Mao, Hongzhi Qi, Liping Yang, Qin Zhou, Mengxiao Tian, Wenbao Zhang, Yongxing Bao
Acta Parasitologica.2022; 67(2): 820. CrossRef
Transcriptome analysis uncovers the key pathways and candidate genes related to the treatment of Echinococcus granulosus protoscoleces with the repurposed drug pyronaridine
Yingfang Yu, Jun Li, Weisi Wang, Tian Wang, Wenjing Qi, Xueting Zheng, Lei Duan, Jiaxu Chen, Shizhu Li, Xiumin Han, Wenbao Zhang, Liping Duan
BMC Genomics.2021;[Epub] CrossRef
Knock Down the Egp38 and Combine with Radiation to Increase Its Inhibitory Effect on Echinococcus granulosus
Pengfei Lu, Mengxiao Tian, Na Yi, Rui Mao, Hongzhi Qi, Liping Yang, Qin Zhou, Jun Li, Wenbao Zhang, Yongxing Bao
SSRN Electronic Journal .2021;[Epub] CrossRef
Identification of Functional MKK3/6 and MEK1/2 Homologs from Echinococcus granulosus and Investigation of Protoscolecidal Activity of Mitogen-Activated Protein Kinase Signaling Pathway Inhibitors In Vitro and
Chuanshan Zhang, Jing Li, Tuerganaili Aji, Liang Li, Xiaojuan Bi, Ning Yang, Zhide Li, Hui Wang, Rui Mao, Guodong Lü, Yingmei Shao, Dominique A. Vuitton, Hao Wen, Renyong Lin
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef
Echinococcosis: Advances in the 21st Century
Hao Wen, Lucine Vuitton, Tuerhongjiang Tuxun, Jun Li, Dominique A. Vuitton, Wenbao Zhang, Donald P. McManus
Clinical Microbiology Reviews.2019;[Epub] CrossRef

9,560 View
146 Download
5 Web of Science
Crossref

Differential Expression of Hox and Notch Genes in Larval and Adult Stages of Echinococcus granulosus: Ebrahim Saedi Dezaki, Mohammad Mehdi Yaghoobi, Elham Taheri, Pooya Ghaseminejad Almani, Farideh Tohidi, Bruno Gottstein, Majid Fasihi Harandi; Korean J Parasitol 2016;54(5):653-658.
Published online October 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.5.653

Abstract
PDF

This investigation aimed to evaluate the differential expression of HoxB7 and notch genes in different developmental stages of Echinococcus granulosus sensu stricto. The expression of HoxB7 gene was observed at all developmental stages. Nevertheless, significant fold differences in the expression level was documented in the juvenile worm with 3 or more proglottids, the germinal layer from infected sheep, and the adult worm from an experimentally infected dog. The notch gene was expressed at all developmental stages of E. granulosus; however, the fold difference was significantly increased at the microcysts in monophasic culture medium and the germinal layer of infected sheep in comparison with other stages. The findings demonstrated that the 2 aforementioned genes evaluated in the present study were differentially expressed at different developmental stages of the parasite and may contribute to some important biological processes of E. granulosus.

Citations

Citations to this article as recorded by

Evolution and Function of the Notch Signaling Pathway: An Invertebrate Perspective
Yan Lv, Xuan Pang, Zhonghong Cao, Changping Song, Baohua Liu, Weiwei Wu, Qiuxiang Pang
International Journal of Molecular Sciences.2024; 25(6): 3322. CrossRef
Echinococcus multilocularis delta/notch signalling components are expressed in post-mitotic cells
Chris Speicher, Monika Bergmann, Klaus Brehm
Parasitology Research.2024;[Epub] CrossRef
Notch signaling pathway involved in Echinococcus granulosus infection regulates dendritic cell development and differentiation
Mingxia Wang, Zailing Shang, Fei Qiao, Junhu Hei, Xueling Ma, Yana Wang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis
Guilherme Brzoskowski dos Santos, Edileuza Danieli da Silva, Eduardo Shigueo Kitano, Maria Eduarda Battistella, Karina Mariante Monteiro, Jeferson Camargo de Lima, Henrique Bunselmeyer Ferreira, Solange Maria de Toledo Serrano, Arnaldo Zaha
Parasites & Vectors.2022;[Epub] CrossRef
The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis
Raúl J. Bobes, Karel Estrada, Diana G. Rios-Valencia, Arturo Calderón-Gallegos, Patricia de la Torre, Julio C. Carrero, Alejandro Sanchez-Flores, Juan P. Laclette
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Bioinformatics analysis and experimental verification of Notch signalling pathway-related miRNA–mRNA subnetwork in extracellular vesicles during Echinococcus granulosus encystation
Jian Gao, Xuan Zhou, Ling Liu, Guodong Lv, Qiulian Hou, Xiaofan Zhang, Yujuan Shen
Parasites & Vectors.2022;[Epub] CrossRef
Screening, construction, and serological identification of the diagnostic antigen molecule EG-06283 for the diagnosis of Echinococcus granulosus
Yazhou Zhu, Tingrui Zhang, Yinqi Zhao, Yin Wang, Yongxue Lv, Shasha Li, Songhao Yang, Mingxing Zhu, Wei Zhao, Qiang Wang
Parasitology Research.2021; 120(7): 2557. CrossRef
Evolutionary Transformations of the Metazoan Body Plan: Genomic-Morphogenetic Correlations
V. V. Isaeva, S. V. Rozhnov
Paleontological Journal.2021; 55(7): 811. CrossRef
In vitro effects of glutathione on Transforming Growth Factor beta and Epidermal Growth Factor Receptor genes expression in the protoscoleces and strobilated worms of Echinococcus granulosus
Seyyed Jafar Nosratabadi, Nasim Hayati Roodbari, Ali Afgar, Mohammad Hossein Modarressi, Alireza Farsinejad, Majid Fasihi Harandi
Gene Reports.2020; 20: 100786. CrossRef
Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti
T. Basika, G.P. Paludo, F.M. Araujo, A.C. Salim, F. Pais, L. Maldonado, N. Macchiaroli, J. Camargo de Lima, M. Rosenzvit, G.C. Oliveira, L. Kamenetzky, H.B. Ferreira
Molecular and Biochemical Parasitology.2019; 229: 35. CrossRef
Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus
Seyed Mohammad Mousavi, Ali Afgar, Mohammad Ali Mohammadi, Seifollah Mortezaei, Balal Sadeghi, Majid Fasihi Harandi
Scientific Reports.2019;[Epub] CrossRef
The effect of albendazole sulfoxide on the expression of miR-61 and let-7 in different in vitro developmental stages of Echinococcus granulosus
Seifollah Mortezaei, Ali Afgar, Mohammad Ali Mohammadi, Seyed Mohammad Mousavi, Balal Sadeghi, Majid Fasihi Harandi
Acta Tropica.2019; 195: 97. CrossRef
Comparative Transcriptomic Analysis of the Larval and Adult Stages of Taenia pisiformis
Shaohua Zhang
Genes.2019; 10(7): 507. CrossRef

9,516 View
191 Download
15 Web of Science
Crossref

Brief Communication

Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran: Mahmoud Agholi, Shahrbanou Naderi Shahabadi, Mohammad Hossein Motazedian, Gholam Reza Hatam; Korean J Parasitol 2016;54(3):339-344.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.339

Abstract
PDF

The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.

Citations

Citations to this article as recorded by

Simultaneous Detection of Sarcocystis hominis, S. heydorni, and S. sigmoideus in Human Intestinal Sarcocystosis, France, 2021–2024
Maxime Moniot, Patricia Combes, Damien Costa, Nicolas Argy, Marie-Fleur Durieux, Thomas Nicol, Céline Nourrisson, Philippe Poirier
Emerging Infectious Diseases.2025;[Epub] CrossRef
Unveiling the hidden threat: prevalence of Enterocytozoon bieneusi and other intestinal parasitic infections among organ transplant recipients in Southern Iran
Mohammad Hossein Motazedian, Leila Morabi, Shahrokh Ezzatzadegan Jahromi, Mojtaba Shafiekhani, Saeed Shahabi, Hamed Nikoupour Dailami, Siavash Gholami, Bahador Sarkari
Gut Pathogens.2025;[Epub] CrossRef
Molecular characterisation of sarcosporidiosis in cattle of Duhok governorate in Kurdistan region of Iraq
S. N. Hussein, M. S. Shukur, A. A. Ibrahim
BULGARIAN JOURNAL OF VETERINARY MEDICINE.2025; 28(2): 210. CrossRef
Worldwide Epidemiology of Cyclospora cayetanensis in HIV/AIDS Patients: A Systematic Review and Meta-Analysis
Farzad Mahdavi, Ali Pouryousef, Mohammad Reza Mohammadi, Kambiz Karimi, Asma Mousivand, Farajolah Maleki, Ali Asghari, Mina Mamizadeh
Acta Parasitologica.2025;[Epub] CrossRef
Prevalence and Associated Risk Factors of Cyclospora cayetanensis in Immunocompromised Patients: A Systematic Review and Meta‐Analysis
Ali Ghorbani, Rostam Menati, Farshad Kakian, Ali Pouryousef, Asma Mousivand, Kambiz Karimi, Farajolah Maleki, Ali Asghari, Jalil Feizi, Panagiotis Karanis
Canadian Journal of Infectious Diseases and Medical Microbiology.2025;[Epub] CrossRef
Infectious etiologies of persistent and chronic diarrhea in Asian developing countries: A systematic review and meta‐analysis
Matthew Chung Yi Koh, Shi Ni Tan, Jinghao Nicholas Ngiam, Paul Anantharajah Tambyah, Kewin Tien Ho Siah
Journal of Gastroenterology and Hepatology.2024; 39(9): 1760. CrossRef
Microscopic and Molecular Identification of Cyclospora cayetanensis and Cystoisospora belli in HIV-Infected People in Tabriz, Northwest of Iran
Saba Ramezanzadeh, Gholamreza Barzegar, Hamid Owaysee Osquee, Majid Pirestani, Mahmoud Mahami-Oskouei, Maryam Hajizadeh, Seyed Abdollah Hosseini, Sonia M. Rodrigues Oliveira, Mahmoud Agholi, Maria de Lourdes Pereira, Ehsan Ahmadpour
Tropical Medicine and Infectious Disease.2023; 8(7): 368. CrossRef
Prevalence of Intestinal Parasitic Diseases in HIV Patients in Tabriz(Original article)
Saba Ramezanzadeh, Golam Reza Barzegar, Hamid Oveisi Oskouei, Majid Pirestani, Mahmoud Mahami-Oskouei, Farzaneh Jafarian, Ehsan Ahmadpour, Seyed Abdollah Hosseini
Journal of Ardabil University of Medical Sciences.2023; 23(4): 352. CrossRef
Global Burden of Cyclospora cayetanensis Infection and Associated Risk Factors in People Living with HIV and/or AIDS
Saba Ramezanzadeh, Apostolos Beloukas, Abdol Sattar Pagheh, Mohammad Taghi Rahimi, Seyed Abdollah Hosseini, Sonia M. Rodrigues Oliveira, Maria de Lourdes Pereira, Ehsan Ahmadpour
Viruses.2022; 14(6): 1279. CrossRef
Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq
Shadan Hassan Abdullah
Veterinary World.2021; 14(2): 468. CrossRef
Prevalence of intestinal microsporidiosis by morphological and molecular methods in eastern Iran
Mahmoodreza Behravan, Mustapha Ahmed Yusuf, Rahmat Solgi, Ali Haghighi
Gene Reports.2021; 24: 101296. CrossRef
Comparison of Two Real-Time PCR Assays Targeting Ribosomal Sequences for the Identification of Cystoisospora belli in Human Stool Samples
Martin Blohm, Andreas Hahn, Ralf Matthias Hagen, Kirsten Alexandra Eberhardt, Holger Rohde, Gérard Leboulle, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt
Pathogens.2021; 10(8): 1053. CrossRef
Molecular differentiation of cattle Sarcocystis spp. by multiplex PCR targeting 18S and COI genes following identification of Sarcocystis hominis in human stool samples
S. Rubiola, T. Civera, E. Ferroglio, S. Zanet, T. Zaccaria, S. Brossa, R. Cipriani, F. Chiesa
Food and Waterborne Parasitology.2020; 18: e00074. CrossRef
Sarcocystosis in Ruminants of Iran, as Neglected Food-Borne Disease: A Systematic Review and Meta-analysis
Davood Anvari, Elahe Narouei, Mona Hosseini, Mohammad Reza Narouei, Ahmad Daryani, Seyyed Ali Shariatzadeh, Abdol Sattar Pagheh, Shirzad Gholami, Shahabeddin Sarvi, Dariush Sargazi, Reza Saberi, Seyed Abdollah Hosseini, Abolghasem Siyadatpanah
Acta Parasitologica.2020; 65(3): 555. CrossRef
Cystoisospora belliinfections in humans: the past 100 years
J. P. Dubey, S. Almeria
Parasitology.2019; 146(12): 1490. CrossRef
Sarcocystis spp. in Romanian Slaughtered Cattle: Molecular Characterization and Epidemiological Significance of the Findings
Kálmán Imre, Gheorghe Dărăbuș, Emil Tîrziu, Sorin Morariu, Mirela Imre, Judit Plutzer, Marius V. Boldea, Adriana Morar
BioMed Research International.2019; 2019: 1. CrossRef
First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt
Asmaa M. El-kady, Nermean M. Hussein, Amal A. Hassan
Journal of Parasitic Diseases.2018; 42(1): 114. CrossRef
Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Seobo Sim, Jua Won, Jae-Whan Kim, Kyungjin Kim, Woo-Yoon Park, Jae-Ran Yu
The Korean Journal of Parasitology.2017; 55(2): 137. CrossRef
Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran
Mahmoud Rahdar, Tahereh Kardooni
Jundishapur Journal of Microbiology.2017;[Epub] CrossRef
Human intestinal sarcocystosis in Iran: there but not seen
Mahmoud Agholi, Zahra Taghadosi, Davood Mehrabani, Farzaneh Zahabiun, Zahra Sharafi, Mohammad Hossein Motazedian, Gholam Reza Hatam, Shahrbanou Naderi Shahabadi
Parasitology Research.2016; 115(12): 4527. CrossRef

12,324 View
149 Download
20 Web of Science
Crossref

Original Article

Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2016;54(2):133-138.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.133

Abstract
PDF

Encystation is an essential process for Acanthamoeba survival under nutrient-limiting conditions and exposure to drugs. The expression of several genes has been observed to increase or decrease during encystation. Epigenetic processes involved in regulation of gene expression have been shown to play a role in several pathogenic parasites. In the present study, we identified the protein arginine methyltransferase 5 (PRMT5), a known epigenetic regulator, in Acanthamoeba castellanii. PRMT5 of A. castellanii (AcPRMT5) contained domains found in S-adenosylmethionine-dependent methyltransferases and in PRMT5 arginine-N-methyltransferase. Expression levels of AcPRMT5 were increased during encystation of A. castellanii. The EGFP-PRMT5 fusion protein was mainly localized in the nucleus of trophozoites. A. castellanii transfected with siRNA designed against AcPRMT5 failed to form mature cysts. The findings of this study lead to a better understanding of epigenetic mechanisms behind the regulation of encystation in cyst-forming pathogenic protozoa.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease
Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui
Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Free-living amoebae and squatters in the wild: ecological and molecular features
Ascel Samba-Louaka, Vincent Delafont, Marie-Hélène Rodier, Estelle Cateau, Yann Héchard
FEMS Microbiology Reviews.2019; 43(4): 415. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii
Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2017; 55(2): 109. CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef

10,215 View
103 Download
10 Web of Science
Crossref

Brief Communications

Environmental Contamination by Echinococcus granulosus sensu lato Eggs in Relation to Slaughterhouses in Urban and Rural Areas in Tunisia: Raja Cha?bane-Banaoues, Myriam Oudni-M’rad, Selim M’rad, Habib Mezhoud, Hamouda Babba; Korean J Parasitol 2016;54(1):113-118.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.113

Abstract
PDF

Hydatidosis has become a real concern for health care institutions and animal rearers in Tunisia. The Tunisian endemicity is aggravated by the growing number of dogs and the difficulty of getting rid of contaminated viscera because of the lack of equipment in most slaughterhouses. Therefore, microscopic and molecular tools were applied to evaluate the role of slaughterhouses in canine infection and Echinococcus granulosus sensu lato (s. l.) egg dissemination. Exposure risk to E. granulosus s. l. eggs in urban and rural areas was explored in order to implant preventive and adapted control strategies. Microscopic examinations detected taeniid eggs in 152 amongst 553 fecal samples. The copro-PCR demonstrated that 138 of 152 taeniid samples analyzed were positive for E. granulosus s. l. DNA. PCR-RFLP demonstrated that all isolated samples belonged to E. granulosus sensu stricto (s. s.). An important environmental contamination index (25.0%) by E. granulosus s. l. eggs was demonstrated. The average contamination index from the regions around slaughterhouses (23.3%; 95% CI: 17.7-28.9%) was in the same range as detected in areas located far from slaughterhouses (26.0%, 95% CI: 21.3-30.8%). Echinococcosis endemic areas were extended in both rural (29.9%, 95% CI: 24.8-34.9%) and urban locations (18.1%, 95% CI: 13.0-22.9%). The pathogen dissemination is related neither to the presence/absence of slaughterhouses nor to the location in urban or rural areas, but is probably influenced by human activities (home slaughtering) and behavior towards the infected viscera.

Citations

Citations to this article as recorded by

Zoonotic threat of cystic echinococcosis in Tunisia: insights into livestock prevalence and identification of the G1 genotype
Mohamed Hedi Abdelghani, Selim M’rad, Raja Chaâbane-Banaoues, Sayadi Taoufik, Mohamed Ali Charfedine, Lamia Zemzemi, Ines Kamoun, Hamouda Babba, Myriam Oudni-M’rad
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against Echinococcus granulosus in Mice
Yongxue Lv, Jing Tang, Tao Li, Yinqi Zhao, Changyou Wu, Wei Zhao
Vaccines.2025; 13(3): 266. CrossRef
Environmental contamination with feces of free-roaming dogs and the risk of transmission of Echinococcus and Taenia species in urban regions of southeastern Iran
Saeedeh Shamsaddini, Carina Schneider, Sonja Dumendiak, Hossein Aghassi, Hossein Kamyabi, Elham Akhlaghi, Marion Wassermann, Majid Fasihi Harandi, Peter Deplazes, Thomas Romig
Parasites & Vectors.2024;[Epub] CrossRef
Global distribution of Echinococcus granulosus genotypes in domestic and wild canids: a systematic review and meta-analysis
Morteza Shams, Sasan Khazaei, Razi Naserifar, Seyyed Ali Shariatzadeh, Davood Anvari, Fattaneh Montazeri, Majid Pirestani, Hamidreza Majidiani
Parasitology.2022; 149(9): 1147. CrossRef
Detection of Echinococcus granulosus sensu lato in Environmental Samples from Ibadan, Oyo State, South West Nigeria
Emmanuel Jolaoluwa Awosanya, Adeola Olagbaju, Angela Peruzzu, Gabriella Masu, Giovanna Masala, Piero Bonelli
Veterinary Sciences.2022; 9(12): 679. CrossRef
Human and animal cystic echinococcosis in Tataouine governorate: hypoendemic area in a hyperendemic country, myth or reality?
Selim M’rad, Raja Chaâbane-Banaoues, Massaouda Ghrab, Hamouda Babba, Myriam Oudni-M’rad
Parasites & Vectors.2021;[Epub] CrossRef
Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina
P. Sánchez Thevenet, H.M. Alvarez, C. Torrecillas, O. Jensen, J.A. Basualdo
Journal of Helminthology.2020;[Epub] CrossRef
Molecular identification of Echinococcus granulosus sensu lato by mitochondrial COX1 and SSU-rDNA markers in dogs in the west of Iran
Seyed-Reza Mirbadie, Masoomeh Zivdari, Hamed Kalani, Mohammad-Reza Vafaei, Shahrokh Izadi, Zahra Jabalameli, Mohsen Mohammadi, Ganesh Yadagiri, Peyman Heydarian, Farzaneh Mirzaei, Mohammad-Ali Mohaghegh
Gene Reports.2020; 19: 100616. CrossRef
Reinventing the Wheel of Echinococcus granulosus sensu lato Transmission to Humans
Francesca Tamarozzi, Peter Deplazes, Adriano Casulli
Trends in Parasitology.2020; 36(5): 427. CrossRef
Parasitological Contamination of Vegetables Sold in Tunisian Retail Markets with Helminth Eggs and Protozoan Cysts
Selim M'rad, Raja Chaabane-Banaoues, Ibtissem Lahmar, Hamza Oumaima, Habib Mezhoud, Hamouda Babba, Myriam Oudni-M'rad
Journal of Food Protection.2020; 83(7): 1104. CrossRef
Environmental, climatic and host population risk factors of human cystic echinococcosis in southwest of Iran
Mohammad Amin Ghatee, Koorosh Nikaein, Walter Robert Taylor, Mehdi Karamian, Hasan Alidadi, Zahra Kanannejad, Faezeh Sehatpour, Fateme Zarei, Gholamreza Pouladfar
BMC Public Health.2020;[Epub] CrossRef
Molecular phylodiagnosis of Echinococcus granulosus sensu lato and Taenia hydatigena determined by mitochondrial Cox1 and SSU-rDNA markers in Iranian dogs: Indicating the first record of pig strain (G7) in definitive host in the Middle East
Seyed Reza Mirbadie, Abbas Najafi Nasab, Mohammad Ali Mohaghegh, Pirasteh Norouzi, Mehdi Mirzaii, Adel Spotin
Comparative Immunology, Microbiology and Infectious Diseases.2019; 65: 88. CrossRef
Molecular characterization of Echinococcus species in dogs from four regions of Kenya
Erastus Mulinge, Japhet Magambo, David Odongo, Sammy Njenga, Eberhard Zeyhle, Cecilia Mbae, Dorothy Kagendo, Francis Addy, Dennis Ebi, Marion Wassermann, Peter Kern, Thomas Romig
Veterinary Parasitology.2018; 255: 49. CrossRef
Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey
Bekir Oguz, Nalan Ozdal, Ozlem Orunc Kilinc, M. Serdar Deger
Journal of Veterinary Research.2018; 62(4): 497. CrossRef
Hydatid Recurrence Medically Treated by Albendazole
Imen Khammari, Mohamed Amine El Ghali, Salsabil Nasri, Imen Dhib, Hamed Chouaieb, Alia Yaacoub, Moncef Ben Said, Rached Letaief, Akila Fathallah
The Open Parasitology Journal.2018; 6(1): 1. CrossRef
First molecular identification of Echinococcus vogeli and Echinococcus granulosus (sensu stricto) G1 revealed in feces of domestic dogs (Canis familiaris) from Acre, Brazil
Leandro Batista das Neves, Paulo Eduardo Ferlini Teixeira, Sidnei Silva, Fernanda Bittencourt de Oliveira, Daniel Daipert Garcia, Fernanda Barbosa de Almeida, Rosângela Rodrigues-Silva, José Roberto Machado-Silva
Parasites & Vectors.2017;[Epub] CrossRef
Cystic Echinococcosis in the Mediterranean
Tommaso Manciulli, M. Mariconti, A. Vola, R. Lissandrin, E. Brunetti
Current Tropical Medicine Reports.2017; 4(4): 235. CrossRef
Analysis of Lethality in Echinococcal Disease
Anna S. Khachatryan
The Korean Journal of Parasitology.2017; 55(5): 549. CrossRef

11,403 View
121 Download
14 Web of Science
Crossref

Surgical and Molecular Evaluation of Pediatric Hydatid Cyst Cases in Eastern Turkey: Unal Bakal, Sami Simsek, Ahmet Kazez; Korean J Parasitol 2015;53(6):785-788.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.785

Abstract
PDF

Cystic echinococcosis (CE) caused by Echinococcus granulosus is a major public health problem worldwide, including Turkey. The aim of the current study was to identify the strains and to estimate the potential risk factors of E. granulosus in operated pediatric cases in eastern Turkey. Ten pediatric patients (7 boys and 3 girls) living in rural areas, with ages ranging from 3 to 15 years old and various clinical histories, were included in this study. Eight patients had only liver hydatid cyst, while 1 patient had liver and lung hydatid cyst and the other liver, lung, and spleen, together. There were 2 ruptured liver cysts. After surgery, during follow-up, no increase was observed in hemagglutination levels, there were no mortalities, and there was no evidence of recurrence at 2 years post operation in all patients. Molecular analysis was performed on hydatid cyst samples obtained from the 10 pediatric cases. According to mt-12S rRNA PCR results, all cases were found to be G1/G3 cluster of E. granulosus sensu stricto.

Citations

Citations to this article as recorded by

Outcomes and Risk Factors for Morbidity After Lung Hydatidosis Surgery in Children
Zied Chaari, Saloua Ammar, Aymen Ben Ayed, Emna Krichen, Aymen Dammak, Jihen Jdidi, Abdessalem Hentati, Riadh Mhiri, Faiza Safi, Imed Frikha
The Journal of Pediatrics.2025; 276: 114367. CrossRef
Cystic Echinococcosis in Hospitalized Children from Western Romania: A 25-Year Retrospective Study
Ana Alexandra Paduraru, Maria Alina Lupu, Calin Marius Popoiu, Maria Corina Stanciulescu, Livius Tirnea, Eugen Sorin Boia, Tudor Rares Olariu
Biomedicines.2024; 12(2): 281. CrossRef
Evaluation of Cases with Pediatric Hydatid Cyst: A 20-years Experience from Turkey
Şenay Erdoğan Durmuş, Cansu Türker, Nuray Kepil, Şenol Emre
Journal of Dr Behcet Uz Children s Hospital.2022; 12(3): 211. CrossRef
An overview of the genetic diversity of Echinococcus granulosus sensu lato in Turkey
Sümeyye BAŞER, Salih MAÇİN, Gülay MAÇİN, Serra ÖRSTEN
Journal of Contemporary Medicine.2022; 12(6): 1025. CrossRef
Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey
Salih Macin, Serra Orsten, Rugıyya Samadzade, Bayram Colak, Hakan Cebeci, Duygu Fındık
Parasitology Research.2021; 120(2): 563. CrossRef
Genetic diversity and haplotypes of paediatric hydatid cyst isolates and first occurrence of E. canadensis (G6/G7) in paediatric cases in Turkey
Unal Bakal, Figen Celik, Sami Simsek, Harun Kaya Kesik, Seyma Gunyakti Kilinc
Parasitology.2021; 148(12): 1482. CrossRef
Echinococcoses in Iran, Turkey, and Pakistan: Old Diseases in the New Millennium
Mehdi Borhani, Saeid Fathi, Enayat Darabi, Fatemeh Jalousian, Sami Simsek, Haroon Ahmed, Harun Kaya Kesik, Seyed Hossein Hosseini, Thomas Romig, Majid Fasihi Harandi, Iraj Mobedi
Clinical Microbiology Reviews.2021;[Epub] CrossRef
A Giant Pulmonary Hydatid Cyst in a 13-Year-Old Child: A Case Report
Nora Bigdeli, Fatemeh Zahra Bagheri, Fatemeh Pouladkhay, Tayebeh Azarmehr, Mehdi Abbasi Sahebi
Medical - Surgical Nursing Journal.2021;[Epub] CrossRef
Molecular survey on cattle and sheep hydatidosis and first detection ofEchinococcus canadensis(G6/G7) in sheep in Turkey
Sarfraz Mehmood, Sami Simsek, Figen Celik, Harun Kaya Kesik, Seyma Gunyakti Kilinc, Haroon Ahmed
Parasitology.2020; 147(9): 1055. CrossRef
Investigation of the relationship between CE cyst characteristics and genetic diversity of Echinococcus granulosus sensu lato in humans from Turkey
Serra Örsten, Türkmen Çiftçi, Aynur Azizova, Gökhan Yüce, Aycan Uysal, Çetin İmamoğlu, Ergun Karaağaoğlu, Devrim Akıncı, Yakut Akyön, Adriano Casulli, Okan Akhan
Parasitology.2020; 147(14): 1712. CrossRef
Human cystic echinococcosis in Turkey: a preliminary study on DNA polymorphisms of hydatid cysts removed from confirmed patients
Serra Orsten, Belgees Boufana, Turkmen Ciftci, Devrim Akinci, Ergun Karaagaoglu, Cumhur Ozkuyumcu, Adriano Casulli, Okan Akhan
Parasitology Research.2018; 117(4): 1257. CrossRef
Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients
Irina Marinova, Markus Spiliotis, Junhua Wang, Marin Muhtarov, Ilias Chaligiannis, Smaro Sotiraki, Iskra Rainova, Bruno Gottstein, Ghalia Boubaker
Parasitology Research.2017; 116(3): 1043. CrossRef
Cystic Echinococcosis in the Mediterranean
Tommaso Manciulli, M. Mariconti, A. Vola, R. Lissandrin, E. Brunetti
Current Tropical Medicine Reports.2017; 4(4): 235. CrossRef

7,575 View
120 Download
12 Web of Science
Crossref

Prevalence of Cryptosporidium Infection among Inhabitants of 2 Rural Areas in White Nile State, Sudan: Seobo Sim, Jae-Ran Yu, Young-Ha Lee, Jin-Su Lee, Hoo-Gn Jeong, Abd Al Wahab Saed Mohamed, Sung-Tae Hong; Korean J Parasitol 2015;53(6):745-747.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.745

Abstract
PDF

Cryptosporidium, a protozoan parasite that causes watery diarrhea, is found worldwide and is common in areas with low water hygiene. In February 2014, 866 stool samples were collected from the inhabitants of 2 rural areas in White Nile State, Sudan. These stool samples were assessed by performing modified acid-fast staining, followed by examination under a light microscope. The overall positive rate of Cryptosporidium oocysts was 13.3%. Cryptosporidium oocysts were detected in 8.6% stool samples obtained from inhabitants living in the area having water purification systems and in 14.6% stool samples obtained from inhabitants living in the area not having water purification systems. No significant difference was observed in the prevalence of Cryptosporidium infection between men and women (14.7% and 14.1%, respectively). The positive rate of oocysts by age was the highest among inhabitants in their 60s (40.0%). These findings suggest that the use of water purification systems is important for preventing Cryptosporidium infection among inhabitants of these rural areas in Sudan.

Citations

Citations to this article as recorded by

Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study
Timothé Guilavogui, Nausicaa Gantois, Jérémy Desramaut, Fode Ibrahima Cissé, Salif Cherif Touré, Bakary Luther Kourouma, Cristian Preda, Magali Chabé, Eric Viscogliosi, Gabriela Certad
Parasite.2024; 31: 70. CrossRef
Prevalence of cryptosporidiosis among children with diarrhoea under five years admitted to Kosti teaching hospital, Kosti City, Sudan
Abdelhakam G. Tamomh, AbdElhadi M. Agena, Elham Elamin, Mohammed A. Suliman, Mohammed Elmadani, Asmaa B. Omara, Sahar A. Musa
BMC Infectious Diseases.2021;[Epub] CrossRef
First report and molecular characterization of Cryptosporidium spp. in humans and animals in Khartoum state, Sudan
Kaltoum Yagoub Adam, A. A. Ismail, M. A. Masri, A. A. Gameel
Veterinary World.2019; 12(1): 183. CrossRef
Epidemiological Survey on Schistosomiasis and Intestinal Helminthiasis among Village Residents of the Rural River Basin Area in White Nile State, Sudan
Young-Ha Lee, Jin-Su Lee, Hoo-Gn Jeoung, In-Sun Kwon, Abd Al Wahab Saed Mohamed, Sung-Tae Hong
The Korean Journal of Parasitology.2019; 57(2): 135. CrossRef
Cryptosporidium and Giardia in Africa: current and future challenges
Sylvia Afriyie Squire, Una Ryan
Parasites & Vectors.2017;[Epub] CrossRef
Cryptosporidium spp. and rotavirus gastroenteritis and change of incidence after rotavirus vaccination among children in Raparin Pediatrics Hospital, Erbil, Iraq
Sally S. Azeez, Hadi M. Alsakee
Medical Journal of Indonesia.2017; 26(3): 190. CrossRef

9,196 View
97 Download
6 Web of Science
Crossref

Original Article

Prevalence of Serum IgG Antibodies to Cystic Echinococcus Antigen among Patients in an Uzbekistan Emergency Hospital: Se Jin Park, Sung Sik Han, Khikmat Anvarov, Abdukhakim Khajibaev, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2015;53(6):699-703.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.699

Abstract
PDF

Cystic echinococcosis (CE) is one of the most widespread zoonotic helminthiases, which can last an asymptomatic infection for several years. The purpose of this study was to demonstrate serum antibody prevalence of CE among asymptomatic people in Uzbekistan using ELISA. A total of 2,547 serum samples were collected, 66 from confirmed CE patients and 2,481 of patients with other diseases than CE at a hospital in Tashkent, Uzbekistan. The serum samples were screened for CE specific IgG antibodies by ELISA using cystic fluid antigen obtained from sheep. The serum antibody positive rate was 89.4% (59/66) in CE and 3.6% (89/2,481) in other disease patients. The present ELISA recognized 89.4% sensitivity and 96.4% specificity. The ELISA absorbance of positive samples was distributed 0.271-0.971 for CE and 0.273-0.887 for other disease patients. The other disease patients with high absorbance over 0.3 were 50 (2.0%) who were presumed to be active CE patients. The patients in their 40s showed the highest positive rate of 5.2% (P=0.181), and women were 4.4% while men were 3.1% positive (P=0.136). The data confirmed that there are many asymptomatic patients of CE in Tashkent. It is indicated that CE is an endemic disease of public health importance in Uzbekistan.

Citations

Citations to this article as recorded by

Comparison of Methods in the Serologic Diagnosis of Cystic Echinococcosis
Sidre Erganis, Fakhriddin Sarzhanov, Funda Doğruman Al, Kayhan Cağlar
Acta Parasitologica.2024; 69(2): 1122. CrossRef
The echinococcoses in Asia: The present situation
Akira Ito, Christine M. Budke
Acta Tropica.2017; 176: 11. CrossRef
Kistik Ekinokokoz’un serolojik tanısında Indirekt Hemaglütinasyon, İndirekt Floresan Antikor ve Enzim İmmuno Assay testlerinin etkinliğinin değerlendirilmesi
Sadık AKGÜN, Hakan Sezgin SAYİNER, Tekin KARSLIGİL
Journal of Contemporary Medicine.2017;[Epub] CrossRef

8,295 View
85 Download
3 Web of Science
Crossref

Brief Communication

Prevalence of Cystic Echinococcosis in Slaughtered Sheep as an Indicator to Assess Control Progress in Emin County, Xinjiang, China: Shijie Yang, Weiping Wu, Tian Tian, Jiangshan Zhao, Kang Chen, Qinyan Wang, Zheng Feng; Korean J Parasitol 2015;53(3):355-359.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.355

Abstract
PDF

Hydatid disease imposing serious threat on human health and great loss in live?stock pastoralism remains a major public health problem in western China. To assess and monitor the effect of control program on transmission dynamics, we used the prevalence of cystic echinococcosis in slaughtered sheep at slaughterhouse as an indicator during the period of 2007 to 2013 in Emin County, Xinjiang Uygur Autonomous Region, China. The results showed a significant decline trend of prevalence in all age groups during the 7 years when the control program was implemented; particularly, the rate was reduced by 72% after first 3 years. Among the sheep slaughtered, the age distribution evidenced that the prevalence increased significantly as the sheep grew older. The baseline data indicated that the rate was 4.5% at the age <1, 6.7% at age 2~, and reached to the highest 17.9% at age ≥4 years. Earlier response to the intervention pressure was seen in the sheep at the younger age. Significant decline started from 2008 at the age <1, from 2009 at age of 1~, 2010 at 2~ to 3~, and the latest, in 2012 at age ≥4. This study demonstrated that the prevalence of cystic echinococcosis in slaughtered sheep may be used as an indicator to assess and monitor the transmission status during and after control program providing information for betterment of performance to sustain control strength.

Citations

Citations to this article as recorded by

Epidemiological and genotypic assessment of cystic echinococcosis in ruminant populations of Northern Punjab, Pakistan: a neglected zoonotic disease
Sakandar Khan, Jo Cable, Numair Masud, Frank Hailer, Muhammad Younus, Naveed Hussain, Muhammad Asif Idrees, Muhammad Imran Rashid, Haroon Akbar
Parasitology Research.2025;[Epub] CrossRef
Zoonotic threat of cystic echinococcosis in Tunisia: insights into livestock prevalence and identification of the G1 genotype
Mohamed Hedi Abdelghani, Selim M’rad, Raja Chaâbane-Banaoues, Sayadi Taoufik, Mohamed Ali Charfedine, Lamia Zemzemi, Ines Kamoun, Hamouda Babba, Myriam Oudni-M’rad
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Advances and prospective in diagnosis of hydatidosis in farm animals
Faten A. M. Abo-Aziza
Journal of Parasitic Diseases.2025; 49(4): 917. CrossRef
Benefits of public awareness in mitigating cystic echinococcosis risk in Western China: A climate and socio-economic perspective
Fang Yin, Wenrui Meng, Peiwei Fan, Yue Shi, Shuai Chen, Yongchun Liang, Jianyi Yao, Yeping Wang, Chuizhao Xue, Shuai Han, Mengmeng Hao, Qian Wang, Ze Meng, Jun Zhuo, Kai Sun, Yongqing Bai, Tingting Kang, Zhenyu Wang, Lei Liu, Dong Jiang, Liqun Fang, Canju
PLOS Neglected Tropical Diseases.2025; 19(7): e0013182. CrossRef
The role of cattle in the transmission of cystic echinococcosis in a highly endemic area of the Peruvian Andes
J. Raúl Lucas, Miguel Cabrera, Néstor Falcón, Orlando Lucas, Alejandro Rodríguez, Daphne Ramos, Cesar Gavidia
Veterinary Parasitology: Regional Studies and Reports.2024; 51: 101021. CrossRef
Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
Nigus Abebe Shumuye, Li Li, John Asekhaen Ohiolei, Sayed Ajmal Qurishi, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Sheng-Zhi Gao, Fu-Heng Zhang, Xue-Qi Tian, Wen-Jun Tian, Yong Fu, Xie-Zhong Wang, Yong-Hong Pan, Fang Zhan, Lin-Sheng Zhang, Min
Parasitology Research.2023; 122(5): 1107. CrossRef
Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
Pathogens.2023; 12(1): 134. CrossRef
EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep
Xiaoli Zhang, Chenxi Wei, Yajie Lv, Rongsheng Mi, Baoping Guo, Sajid Ur Rahman, Yehua Zhang, Long Cheng, Haiyan Jia, Yan Huang, Xiangan Han, Haiyan Gong, Zhaoguo Chen
Microbial Pathogenesis.2023; 179: 106110. CrossRef
Modeling and analysis of the transmission dynamics of cystic echinococcosis: Effects of increasing the number of sheep
Yiwei He, Qianqian Cui, Zengyun Hu
Mathematical Biosciences and Engineering.2023; 20(8): 14596. CrossRef
Cystic Echinococcosis: An Impact Assessment of Prevention Programs in Endemic Developing Countries in Africa, Central Asia, and South America
Elias Christofi, Hafiz Ishfaq Ahmad
Journal of Zoological Systematics and Evolutionary Research.2022; 2022: 1. CrossRef
An integrated approach to control Cystic Echinococcosis in southern Italy
G. Cringoli, P. Pepe, A. Bosco, M.P. Maurelli, L. Baldi, P. Ciaramella, V. Musella, M.L. Buonanno, F. Capuano, F. Corrado, D. Ianniello, L.C. Alves, P. Sarnelli, L Rinaldi
Veterinary Parasitology.2021; 290: 109347. CrossRef
Advances in research on echinococcoses epidemiology in China
Mei-Hua Fu, Xu Wang, Shuai Han, Ya-Yi Guan, Robert Bergquist, Wei-Ping Wu
Acta Tropica.2021; 219: 105921. CrossRef
Meta-Analysis of the Prevalence of Echinococcus in Sheep in China From 1983 to 2020
Yang Gao, Wei Wang, Chuang Lyu, Xin-Yu Wei, Yu Chen, Quan Zhao, Zhi-Guang Ran, You-Qing Xia
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Analysis of Factors Influencing Cystic Echinococcosis in Northwest Non-Qinghai Tibetan Plateau Regions of China
Xiangman Zeng, Yayi Guan, Weiping Wu, Liying Wang, Huixia Cai, Qi Fang, Shicheng Yu, Canjun Zheng
The American Journal of Tropical Medicine and Hygiene.2020; 102(3): 567. CrossRef
Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
Baoping Guo, Zhuangzhi Zhang, Xueting Zheng, Yongzhong Guo, Gang Guo, Li Zhao, Ren Cai, Bingjie Wang, Mei Yang, Xi Shou, Wenbao Zhang, Bin Jia
The Korean Journal of Parasitology.2019; 57(2): 153. CrossRef
Control of cystic echinococcosis: Background and prospects
Edmundo Larrieu, Cesar M. Gavidia, Marshall W. Lightowlers
Zoonoses and Public Health.2019; 66(8): 889. CrossRef
Economic Effect of Confiscation of Cattle Viscera Infected with Cystic Echinococcosis, Huancayo Province, Peru
J. Raúl Lucas, Carmen A. Arias, Stephanie S. Balcázar-Nakamatsu, Alejandro P. Rodríguez, Karen A. Alroy, Cesar M. Gavidia
Emerging Infectious Diseases.2019; 25(12): 2278. CrossRef
CYSTIC HYDATIDOSIS IN KIDNEY OF SHEEP: PREVALENCE AND HISTOPATHOLOGICAL STUDY
Muslimah, N. ALsulami, Nafisa Mohammd Batarfi
Journal of Experimental Biology and Agricultural Sciences.2019; 7(5): 505. CrossRef
Development of a colloidal gold immunochromatographic strip based on HSP70 for the rapid detection of Echinococcus granulosus in sheep
Xunhui Zhuo, Yingchao Yu, Xueqiu Chen, Zhuangzhi Zhang, Yi Yang, Aifang Du
Veterinary Parasitology.2017; 240: 34. CrossRef
Novel Interventional Management of Hepatic Hydatid Cyst with Nanosecond Pulses on Experimental Mouse Model
Xinhua Chen, Ruiqing Zhang, Tuerganaili Aji, Yingmei Shao, Yonggang Chen, Hao Wen
Scientific Reports.2017;[Epub] CrossRef
Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction
Rui-Qing Zhang, Xin-Hua Chen, Hao Wen
World Journal of Gastroenterology.2017; 23(45): 7989. CrossRef
Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran
M.T. Rahimi, S. Sarvi, A. Daryani, M. Sharif, E. Ahmadpour, A. Shokri, A. Mizani
Helminthologia.2016; 53(3): 285. CrossRef

9,487 View
97 Download
24 Web of Science
Crossref

Case Report

A Case of Pneumonia Caused by Pneumocystis jirovecii Resistant to Trimethoprim-Sulfamethoxazole: Sang Min Lee, Yong Kyun Cho, Yon Mi Sung, Dong Hae Chung, Sung Hwan Jeong, Jeong-Woong Park, Sang Pyo Lee; Korean J Parasitol 2015;53(3):321-327.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.321

Abstract
PDF

A 50-year-old male visited the outpatient clinic and complained of fever, poor oral intake, and weight loss. A chest X-ray demonstrated streaky and fibrotic lesions in both lungs, and chest CT revealed multifocal peribronchial patchy ground-glass opacities with septated cystic lesions in both lungs. Cell counts in the bronchoalveolar lavage fluid revealed lymphocyte-dominant leukocytosis, and further analysis of lymphocyte subsets showed a predominance of cytotoxic T cells and few T helper cells. Video-assisted wedge resection of the left upper lobe was performed, and the histologic examination was indicative of a Pneumocystis jirovecii infection. Trimethoprim-sulfamethoxazole (TMP-SMX) was orally administered for 3 weeks; however, the patient complained of cough, and the pneumonia was aggravated in the follow-up chest X-ray and chest CT. Molecular studies demonstrated mutations at codons 55 and 57 of the dihydropteroate synthase (DHPS) gene, which is associated with the resistance to TMP-SMX. Clindamycin-primaquine was subsequently administered for 3 weeks replacing the TMP-SMX. A follow-up chest X-ray showed that the pneumonia was resolving, and the cough was also alleviated. A positive result of HIV immunoassay and elevated titer of HCV RNA indicated HIV infection as an underlying condition. This case highlights the importance of careful monitoring of patients with P. jirovecii pneumonia (PCP) during the course of treatment, and the molecular study of DHPS mutations. Additionally, altering the anti-PCP drug utilized as treatment must be considered when infection with drug-resistant P. jirovecii is suspected. To the best of our knowledge, this is the first case of TMP-SMX-resistant PCP described in Korea.

Citations

Citations to this article as recorded by

Molecular Study of Pneumocystis jirovecii in Respiratory Samples of HIV Patients in Chile
Isabel Iturrieta-González, Carolina Chahin, Johanna Cabrera, Carla Concha, Pamela Olivares-Ferretti, Javier Briones, Fernando Vega, Luis Bustos-Medina, Flery Fonseca-Salamanca
Journal of Fungi.2024; 10(2): 117. CrossRef
Deep mutational scanning of Pneumocystis jirovecii dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolate
Francois D. Rouleau, Alexandre K. Dubé, Isabelle Gagnon-Arsenault, Soham Dibyachintan, Alicia Pageau, Philippe C. Després, Patrick Lagüe, Christian R. Landry, Adrian Serohijos
PLOS Genetics.2024; 20(4): e1011252. CrossRef
Pneumocystis jiroveci Pneumonia: A Review of Management in Human Immunodeficiency Virus (HIV) and Non-HIV Immunocompromised Patients
Atif Ibrahim, Asmi Chattaraj, Qamar Iqbal, Ali Anjum, Mohammad Ebad Ur Rehman, Zobia Aijaz, Fazila Nasir, Sadia Ansar, Tirdad T. Zangeneh, Ahmad Iftikhar
Avicenna Journal of Medicine.2023; 13(01): 023. CrossRef
Molecular mechanisms of acquired antifungal drug resistance in principal fungal pathogens and EUCAST guidance for their laboratory detection and clinical implications
Thomas R Rogers, Paul E Verweij, Mariana Castanheira, Eric Dannaoui, P Lewis White, Maiken Cavling Arendrup, M C Arendrup, S Arikan-Akdagli, F Barchiesi, Jochem Buil, M Castanheira, E Chryssanthou, N Friberg, J Guinea, P Hamal, Ingibjorg Hilmarsdottir, N
Journal of Antimicrobial Chemotherapy.2022; 77(8): 2053. CrossRef
Effectiveness of front line and emerging fungal disease prevention and control interventions and opportunities to address appropriate eco-sustainable solutions
Mary Garvey, Elaine Meade, Neil J. Rowan
Science of The Total Environment.2022; 851: 158284. CrossRef
Chloroquine Analogues as Leads against Pneumocystis Lung Pathogens
Ana Gomes, Ricardo Ferraz, Lauren Ficker, Margaret S. Collins, Cristina Prudêncio, Melanie T. Cushion, Cátia Teixeira, Paula Gomes
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef
Therapy and Management of Pneumocystis jirovecii Infection
P. Lewis White, Jessica S. Price, Matthijs Backx
Journal of Fungi.2018; 4(4): 127. CrossRef
Low prevalence of DHFR and DHPS mutations in Pneumocystis jirovecii strains obtained from a German cohort
Isabelle Suárez, Lisa Roderus, Edeltraud van Gumpel, Norma Jung, Clara Lehmann, Gerd Fätkenheuer, Pia Hartmann, Georg Plum, Jan Rybniker
Infection.2017; 45(3): 341. CrossRef
Cotrimoxazole

Reactions Weekly.2016; 1583(1): 348. CrossRef
La pneumocystose au cours de l’infection à VIH
M. El Fane, M. Sodqi, A. Oulad Lahsen, A. Chakib, L. Marih, K. Marhoum El Filali
Revue de Pneumologie Clinique.2016; 72(4): 248. CrossRef
Managing the oncologic patient with suspected pneumonia in the intensive care unit
D. Leoni, B. Encina, J. Rello
Expert Review of Anti-infective Therapy.2016; 14(10): 943. CrossRef
A Case of Pneumonia Caused byPneumocystis jiroveciiResistant to Trimethoprim/Sulfamethoxazole in the Absence of Previous Drug Exposure
Ari Ahn, Jeonghyun Chang, Heungsup Sung, Mi-Na Kim
Laboratory Medicine Online.2016; 6(4): 250. CrossRef

13,150 View
154 Download
9 Web of Science
Crossref

Original Articles

Subtype Distribution of Blastocystis in Thai-Myanmar Border, Thailand: Supaluk Popruk, Ruenruetai Udonsom, Khuanchai Koompapong, Aongart Mahittikorn, Teera Kusolsuk, Jiraporn Ruangsittichai, Attakorn Palasuwan; Korean J Parasitol 2015;53(1):13-19.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.13

Abstract
PDF

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

Citations

Citations to this article as recorded by

Assessing Zoonotic Risks of Blastocystis Infection in Singapore
Thet Tun Aung, Charlotte Kai Qi How, Jean-Marc Chavatte, Nazmi Bin Nazir, Edgar Macabe Pena, Bryan Ogden, Grace Rou’en Lim, Yasmina Arditi Paramastri, Lois Anne Zitzow, Hanrong Chen, Niranjan Nagarajan, Kevin Shyong Wei Tan, Benoit Malleret
Pathogens.2025; 14(8): 773. CrossRef
Prevalence and subtype distribution of Blastocystis infections among community participants in Thailand: a systematic review and meta-analysis
Manas Kotepui, Supaluk Popruk, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Kinley Wangdi, Aongart Mahittikorn, Christen Rune Stensvold
Parasite.2025; 32: 53. CrossRef
Subtype–host patterns and genetic differentiation of Blastocystis sp. in the Philippines
Davin Edric V. Adao, Windell L. Rivera
Heliyon.2024; 10(7): e29019. CrossRef
Blastocystis colonization and associations with population parameters in Thai adults
Vasana Jinatham, Amara Yowang, Christen Rune Stensvold, Eleni Michalopoulou, Thanakrit Vichasilp, Picha Suwannahitatorn, Siam Popluechai, Anastasios D. Tsaousis, Eleni Gentekaki, Funda Dogruman-Al
PLOS Neglected Tropical Diseases.2024; 18(7): e0012292. CrossRef
Occurrence and Genotypic Identification of Blastocystis spp. and Enterocytozoon bieneusi in Bamaxiang Pigs in Bama Yao Autonomous County of Guangxi Province, China
Xingang Yu, Xuanru Mu, Kaijian Yuan, Sifan Wang, Yilong Li, Hui Xu, Qiaoyu Li, Wenjing Zeng, Zhili Li, Jianchao Guo, Yang Hong
Animals.2024; 14(22): 3344. CrossRef
Phylogenetic relationship of nonmammalian and avian Blastocystis isolates and conventional subtypes
Vanessa Yvonne B. Meclat, Keith Sigfred B. Ancheta, Davin Edric V. Adao, Windell L. Rivera
Journal of Parasitic Diseases.2023; 47(1): 192. CrossRef
Prevalence and Subtype Distribution of Blastocystis Isolated from School-Aged Children in the Thai-Myanmar Border, Ratchaburi Province, Thailand
Amanee Abu, Chantira Sutthikornchai, Aongart Mahittikorn, Khuanchai Koompapong, Rachatawan Chiabchalard, Dumrongkiet Arthan, Ngamphol Soonthornworasiri, Supaluk Popruk
International Journal of Environmental Research and Public Health.2022; 20(1): 204. CrossRef
Prevalence and genotypes/subtypes of Enterocytozoon bieneusi and Blastocystis sp. in different breeds of cattle in Jiangxi Province, southeastern China
Sen Li, Ping Wang, Xing-Quan Zhu, Yang Zou, Xiao-Qing Chen
Infection, Genetics and Evolution.2022; 98: 105216. CrossRef
Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border
Chantira Sutthikornchai, Supaluk Popruk, Aongart Mahittikorn, Dumrongkiet Arthan, Ngamphol Soonthornworasiri, Chirawat Paratthakonkun, Yaoyu Feng, Lihua Xiao
Parasitology Research.2021; 120(8): 2887. CrossRef
Molecular prevalence and subtype distribution ofBlastocystissp. in Asia and in Australia
Sara Nemati, Mohammad Reza Zali, Paul Johnson, Hamed Mirjalali, Panagiotis Karanis
Journal of Water and Health.2021; 19(5): 687. CrossRef
Epidemiology and subtype distribution of Blastocystis in humans: A review
Supaluk Popruk, Davin Edric V. Adao, Windell L. Rivera
Infection, Genetics and Evolution.2021; 95: 105085. CrossRef
The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia
Adedolapo Aminat Rauff-Adedotun, Farah Haziqah Meor Termizi, Nurshafarina Shaari, Ii Li Lee
Biology.2021; 10(10): 990. CrossRef
Prevalence, subtypes and risk factors of Blastocystis spp. infection among pre- and perimenopausal women
Danuta Kosik-Bogacka, Małgorzata Lepczyńska, Karolina Kot, Małgorzata Szkup, Natalia Łanocha-Arendarczyk, Ewa Dzika, Elżbieta Grochans
BMC Infectious Diseases.2021;[Epub] CrossRef
Molecular prevalence and subtypes of Blastocystis sp. in primates in northern China
Lei Ma, Haixiav Qiao, Heqin Wang, Shuai Li, Pupu Zhai, Jing Huang, Yinghui Guo
Transboundary and Emerging Diseases.2020; 67(6): 2789. CrossRef
Prevalence and Subtype Distribution of Blastocystis Infection in Patients with Diabetes Mellitus in Thailand
Noppon Popruk, Satakamol Prasongwattana, Aongart Mahittikorn, Attakorn Palasuwan, Supaluk Popruk, Duangdao Palasuwan
International Journal of Environmental Research and Public Health.2020; 17(23): 8877. CrossRef
Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor Panjang (Macaca fascicularis) di Taman Nasional Baluran, Situbondo, Jawa Timur
Dyah Ayu Kurniawati, Lucia Tri Suwanti, Nunuk Dyah Retno Lastuti, Setiawan Koesdarto, Endang Suprihati, Mufasirin Mufasirin, Arif Pratiwi
Jurnal Medik Veteriner.2020; 3(2): 138. CrossRef
A Simple Genotyping Method for Rapid Differentiation of Blastocystis Subtypes and Subtype Distribution of Blastocystis spp. in Thailand
Nittaya Srichaipon, Surang Nuchprayoon, Sarit Charuchaibovorn, Pattadon Sukkapan, Vivornpun Sanprasert
Pathogens.2019; 8(1): 38. CrossRef
Evaluation of Blastocystis sp. Frequency in Referred Samples to Urban Laboratories: Is it a Potential Risk for Deployed Troops?
Taher Elmi, Fariba Amni, Bahman Rahimi Esboei, Shirzad Gholami, Mostafa Akbariqomi, Mohsen Mortazavi, Mohammad Barati, Fatemeh Tabatabaie
Journal of Archives in Military Medicine.2019;[Epub] CrossRef
Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
Baiyan Gong, Xiaohua Liu, Yanchen Wu, Ning Xu, Meng Xu, Fengkun Yang, Lei Tong, Kexin Zhou, Jianping Cao, Aiqin Liu, Yujuan Shen
Parasite.2019; 26: 46. CrossRef
Blastocystis subtypes detected in long-tailed macaques in Thailand—Further evidence of cryptic host specificity
Kotchaphon Vaisusuk, Weerachai Saijuntha, Sutthira Sedlak, Thongchit Thanchomnang, Warayutt Pilap, Warong Suksavate, Christen Rune Stensvold, Chairat Tantrawatpan
Acta Tropica.2018; 184: 78. CrossRef
Diagnosis and Identification of Blastocystis Subtypes in Primary School Children in Jakarta
Ika P Sari, Martwinny R Benung, Sri Wahdini, Agnes Kurniawan
Journal of Tropical Pediatrics.2018; 64(3): 208. CrossRef
Blastocystis subtype 5: Predominant subtype on pig farms, Thailand
Ai-rada Pintong, Suparat Sunyanusin, Rapeepan Prasertbun, Aongart Mahittikorn, Hirotake Mori, Tanasak Changbunjong, Chalit Komalamisra, Yaowalark Sukthana, Supaluk Popruk
Parasitology International.2018; 67(6): 824. CrossRef
Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes
Carolina Valença Barbosa, Magali Muniz Barreto, Rosemary de Jesus Andrade, Fernando Sodré, Claudia Masini d’Avila-Levy, José Mauro Peralta, Ricardo Pereira Igreja, Heloisa Werneck de Macedo, Helena Lucia Carneiro Santos, Maria Victoria Periago
PLOS ONE.2018; 13(3): e0193860. CrossRef
Genetic diversity analysis of Blastocystis subtypes from both symptomatic and asymptomatic subjects using a barcoding region from the 18S rRNA gene
Tahereh Rezaei Riabi, Hamed Mirjalali, Ali Haghighi, Mohammad Rostami Nejad, Mohammad Amin Pourhoseingholi, Philippe Poirier, Frederic Delbac, Ivan Wawrzyniak, Mohammad Reza Zali
Infection, Genetics and Evolution.2018; 61: 119. CrossRef
Blastocystis subtypes and their association with Irritable Bowel Syndrome
Susana Cifre, Mónica Gozalbo, Vicente Ortiz, José M. Soriano, J. Francisco Merino, María Trelis
Medical Hypotheses.2018; 116: 4. CrossRef
Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand
Ruenruetai Udonsom, Rapeepan Prasertbun, Aongart Mahittikorn, Hirotake Mori, Tanasak Changbunjong, Chalit Komalamisra, Ai-rada Pintong, Yaowalark Sukthana, Supaluk Popruk
Infection, Genetics and Evolution.2018;[Epub] CrossRef
High diversity of Blastocystis subtypes isolated from asymptomatic adults living in Chiang Rai, Thailand
Amara Yowang, Anastasios D. Tsaousis, Tawatchai Chumphonsuk, Nontaphat Thongsin, Niwed Kullawong, Siam Popluechai, Eleni Gentekaki
Infection, Genetics and Evolution.2018; 65: 270. CrossRef
Subtype identification of human Blastocystis spp. isolated from Lao People’s Democratic Republic
Oranuch Sanpool, Sakhone Laymanivong, Tongjit Thanchomnang, Rutchanee Rodpai, Lakkhana Sadaow, Issarapong Phosuk, Wanchai Maleewong, Pewpan M. Intapan
Acta Tropica.2017; 168: 37. CrossRef
Association of Blastocystis subtypes with diarrhea in children
F Zulfa, I P Sari, A Kurniawan
Journal of Physics: Conference Series.2017; 884: 012031. CrossRef
The relation between Blastocystis and the intestinal microbiota in Swedish travellers
Joakim Forsell, Johan Bengtsson-Palme, Martin Angelin, Anders Johansson, Birgitta Evengård, Margareta Granlund
BMC Microbiology.2017;[Epub] CrossRef
Are soil and waterborne parasitic infections health risk for worker populations in southeast Turkey?
Sibel Ak, Fadime Eroğlu, Ali İhsan Nergiz, Furkan Hıyamlı
Dicle Tıp Dergisi.2017; 44(2): 205. CrossRef
Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
Attakorn Palasuwan, Duangdao Palasuwan, Aongart Mahittikorn, Rachatawan Chiabchalard, Valery Combes, Supaluk Popruk
The Korean Journal of Parasitology.2016; 54(4): 455. CrossRef
The distribution of Blastocystis subtypes in isolates from Qatar
Marawan Abu-Madi, Mahmoud Aly, Jerzy M. Behnke, C. Graham Clark, Hanan Balkhy
Parasites & Vectors.2015;[Epub] CrossRef

12,275 View
146 Download
33 Web of Science
Crossref

Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts: Hossein Mahmoudvand, Ebrahim Saedi Dezaki, Farnaz Kheirandish, Behrouz Ezatpour, Sareh Jahanbakhsh, Majid Fasihi Harandi; Korean J Parasitol 2014;52(6):653-659.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.653

Abstract
PDF

Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models.

Citations

Citations to this article as recorded by

Efficacy and mechanism of action of harmine derivative H-2-104 against Echinococcus granulosus infection in mice
Huijing Gao, Qinwei Xu, Jiang Zhu, Kadierya Kuerban, Bei Chen, Jun Zhao, Kalibixiati Aimulajiang, Liang Teng
BMC Veterinary Research.2025;[Epub] CrossRef
Chemical compositions, and biological activities of essential oils from Gomphrena nitida ROTHR from Nigeria
A.A. Sotade, O.O. Aiyelaagbe, O.O. Luchen
Journal of Essential Oil Bearing Plants.2025; 28(3): 564. CrossRef
In vitro evaluation of the susceptibility of Echinococcus granulosus hydatid cysts to lavender and green tea extracts
Seyed Mohammad Mehdizadeh, Farnaz Malekifard, Bijan Esmaeilnejad
Scientific Reports.2025;[Epub] CrossRef
Effects of Lucilia sericata Larval Secretion on Echinococcus granulosus
Feza İrem Aldı, Kıymet Tabakçıoglu, Erdal Polat, Nermin Şakru
Turkish Journal of Parasitology.2025;[Epub] CrossRef
Anthelmintic Effect of Cannabidiol against Echinococcus granulosus sensu stricto
Clara María Albani, Giselle Fuentes, Cristina Lujan Ramírez, Patricia Eugenia Pensel, Florencia Gatti, Adriana Albanese, Diego Nutter, Matías Ezequiel Aguirre, Yésica Dolores Di Iorio, María Celina Elissondo
Tropical Medicine and Infectious Disease.2024; 9(2): 35. CrossRef
Epidemiological study on bovine cystic echinococcosis: Abattoir survey, cyst characterization and its economic impact at Mekaneyesuse municipality abattoir, Northwest Ethiopia
Belete Sendekie, Beyenech Gebeyehu, Abebe Mihret, Tesfu Kassa, Nigatu Kebede
Parasite Epidemiology and Control.2024; 25: e00355. CrossRef
The effect of Nigella sativa oil- and wheat germ oil-loaded metal organic frameworks on chronic murine toxoplasmosis
Omnia Sobhi Mohammad, Heba Mohamed El Naggar, Hagar Fathy Abdelmaksoud, Ashraf Mohamed Barakat, Reda Mohamed Abdelhameed, Mai Abdel Sameaa Shehata
Acta Tropica.2023; 239: 106823. CrossRef
An Inventory of Anthelmintic Plants across the Globe
Haroon Ahmed, Seyma Gunyakti Kilinc, Figen Celik, Harun Kaya Kesik, Sami Simsek, Khawaja Shafique Ahmad, Muhammad Sohail Afzal, Sumaira Farrakh, Waseem Safdar, Fahad Pervaiz, Sadia Liaqat, Jing Zhang, Jianping Cao
Pathogens.2023; 12(1): 131. CrossRef
Eugenol Essential Oil and Nanoemulsion as Antihydatic Agents with Antifibrotic and Immunomodulatory Effects in Cystic Echinococcosis
Alzahraa Abdelraouf Ahmad, Maria Naged Maurice, Mohamed El-Salahy M. Monib, Mahmoud Soliman, Sultan S. Al-Thagfan, Enas Abdelhameed Mahmoud Huseein
Tropical Medicine and Infectious Disease.2023; 8(5): 253. CrossRef
A systematic appraisal of the use of carvacrol-rich plants to treat hydatid cysts
Kourosh Cheraghipour, Leila Masoori, Masoomeh Zivdari, Marjan Beiranvand, Vahid Malekara, Vali Yarahmadi, Kobra Moradpour, Maryam Karkhane, Alaa Kami Alhameedawi, Abdolrazagh Marzban
Journal of Parasitic Diseases.2022; 46(3): 916. CrossRef
Herbal Medicines against Hydatid Disease: A Systematic Review (2000–2021)
Mughees Aizaz Alvi, Sadiq Khan, Rana Muhammad Athar Ali, Warda Qamar, Muhammad Saqib, Noman Yousaf Faridi, Li Li, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Life.2022; 12(5): 676. CrossRef
The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro
Klaudia Ciesielska-Figlon, Agnieszka Daca, Adam Kokotkiewicz, Maria Łuczkiewicz, Bożena Zabiegała, Jacek M. Witkowski, Katarzyna A. Lisowska
Biomedicine & Pharmacotherapy.2022; 153: 113349. CrossRef
Treatment of protoscoleces with gamma radiation: Potential immunoprotective effect against experimental murine echinococcosis
Samia Hadj Rabia, Aicha Debib, Meriem Mezaguer, Rabah Yefsah, Imene Soufli, Bousaad Hamrioui, Chafia Touil‐Boukoffa, Ahsene Baz, Jean Giaimis, Saâdia Mameri
Parasite Immunology.2022;[Epub] CrossRef
Essential Oils as Novel Anthelmintic Drug Candidates
Sujogya Kumar Panda, Marijn Daemen, Gunanidhi Sahoo, Walter Luyten
Molecules.2022; 27(23): 8327. CrossRef
Evaluation of the Apoptotic and Scolicidal Effects of Crude and Flavonoid Extracts of Allium noeanum on Protoscolices and Hydatid Cyst Wall
Zahra Salemi, Mehrdad Goudarzi, Reza Hajihossein, Mitra Noori, Saeid Babaei, Zahra Eslamirad
Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub] CrossRef
Evaluation of the scolicidal activities of eugenol essential oil and its nanoemulsion against protoscoleces of hydatid cysts
Maria Naged Maurice, Enas Abdelhameed Mahmoud Huseein, Mohamed El-Salahy M. M. Monib, Fahd M. Alsharif, Nader Ibrahim Namazi, Alzahraa Abdelraouf Ahmad, Shawky M. Aboelhadid
PLOS ONE.2021; 16(11): e0259290. CrossRef
Antimicrobial and antihelminthic impacts of black cumin, pawpaw and mustard seeds in livestock production and health
Moyosore J. Adegbeye, Mona M. M. Y. Elghandour, Tolulope O. Faniyi, Nallely Rivero Perez, Alberto Barbabosa-Pilego, Adrian Zaragoza-Bastida, Abdelfattah Z. M. Salem
Agroforestry Systems.2020; 94(4): 1255. CrossRef
Anthelmintic activity of crude powder and crude aqueous extract of Trachyspermum ammi on gastrointestinal nematodes in donkey (Equus asinus): An in vivo study
Abbas Imani-Baran, Jalal Abdollahi, Hamid Akbari, Saeed Jafarirad, Sajjad Moharramnejad
Journal of Ethnopharmacology.2020; 248: 112249. CrossRef
In vitro scolicidal activity of Thymus capitatus Hoff. et Link. essential oil on Echinococcus granulosus protoscoleces
Amani Hizem, Selim M’rad, Myriam Oudni-M’rad, Habib Mezhoud, Hichem Ben Jannet, Guido Flamini, Kamel Ghedira, Hamouda Babba
Journal of Essential Oil Research.2020; 32(2): 178. CrossRef
In vitro efficacy study of Cinnamomum zeylanicum essential oil and cinnamaldehyde against the larval stage of Echinococcus granulosus
Julia Fabbri, Marina Alejandra Maggiore, Patricia Eugenia Pensel, Guillermo María Denegri, María Celina Elissondo
Experimental Parasitology.2020; 214: 107904. CrossRef
Main compounds and in vitro effectiveness of Syzygium aromaticum essential oil on protoscoleces of hydatid cyst
Sid Mohammed Ammar Selles, Mokhtaria Kouidri, Tahar Belkacem Belhamiti, Amar Ait Amrane, Mohamed Benahmed, Abdelkader Hachemi
Comparative Clinical Pathology.2020; 29(3): 705. CrossRef
A systematic review of medicinal plants used against Echinococcus granulosus
Rehman Ali, Sanaullah Khan, Marina Khan, Muhammad Adnan, Ijaz Ali, Taj Ali Khan, Sumbal Haleem, Muhammad Rooman, Sadia Norin, Shahid Niaz Khan, Branislav T. Šiler
PLOS ONE.2020; 15(10): e0240456. CrossRef
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro
Ulfet Cetinkaya, Gülay Sezer, Arzuv Charyyeva
Asian Pacific Journal of Tropical Biomedicine.2020; 10(1): 42. CrossRef
The scolicidal activity of the essential oil obtained from the needles of Pinus nigra Arn. subsp. pallasiana (Lamb.) Holmboe on hydatid cyst
Esma Kozan, Mert Ilhan, Ibrahim Tümen, Esra Küpeli Akkol
Journal of Ethnopharmacology.2019; 235: 243. CrossRef
Echinococcosis: Advances in the 21st Century
Hao Wen, Lucine Vuitton, Tuerhongjiang Tuxun, Jun Li, Dominique A. Vuitton, Wenbao Zhang, Donald P. McManus
Clinical Microbiology Reviews.2019;[Epub] CrossRef
In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts
Nasr Moawad El-Bahy, Amer Ragheb Abdelaziz, Reda Elbastawisy Khalafalla
Revista Brasileira de Parasitologia Veterinária.2019; 28(2): 210. CrossRef
Progress in the pharmacological treatment of human cystic and alveolar echinococcosis: Compounds and therapeutic targets
Mar Siles-Lucas, Adriano Casulli, Roberto Cirilli, David Carmena, Giovanna Raso
PLOS Neglected Tropical Diseases.2018; 12(4): e0006422. CrossRef
Could beta-myrcene be an alternative to albendazole for the treatment of experimental cystic echinococcosis?
J. Fabbri, M.A. Maggiore, P.E. Pensel, C.M. Albani, G.M. Denegri, M.C. Elissondo
Acta Tropica.2018; 187: 5. CrossRef
Scolicidal Effects of Gallic Acid, One of the Major Compounds of Plants, on Protoscolices of Hydatid Cyst
Sara Larki, Mohammad Hosein Razi Jalali, Saeed Goodarzi
Zahedan Journal of Research in Medical Sciences.2017;[Epub] CrossRef
Natural products applied against hydatid cyst protoscolices: A review of past to present
Mohammad Hasan Kohansal, Abbasali Nourian, Mohammad Taghi Rahimi, Ahmad Daryani, Adel Spotin, Ehsan Ahmadpour
Acta Tropica.2017; 176: 385. CrossRef
Scolicidal agents for protoscolices of Echinococcus granulosus hydatid cyst: Review of literature
Seyedeh Maryam Sharafi, Raheleh Rafiei Sefiddashti, Behnam Sanei, Morteza Yousefi, Hossein Yousofi Darani
Journal of Research in Medical Sciences.2017;[Epub] CrossRef
Zataria multiflora Bioss: lethal effects of methanolic extract against protoscoleces of Echinococcus granulosus
Sareh Jahanbakhsh, Mojgan Azadpour, Amir Tavakoli Kareshk, Amir Keyhani, Hossein Mahmoudvand
Journal of Parasitic Diseases.2016; 40(4): 1289. CrossRef
Chemical composition, protoscolicidal effects and acute toxicity of Pistacia atlantica Desf. fruit extract
Hossein Mahmoudvand, Farnaz Kheirandish, Mehdi Ghasemi Kia, Amir Tavakoli Kareshk, Mohammad Yarahmadi
Natural Product Research.2016; 30(10): 1208. CrossRef
Effect of Nigella sativa oil on experimental toxoplasmosis
Rasha F. Mady, Wessam El-Hadidy, Samar Elachy
Parasitology Research.2016; 115(1): 379. CrossRef
Efficacy ofMyrtus communisL. to Inactivate the Hydatid Cyst Protoscoleces
Hossein Mahmoudvand, Shirzad Fallahi, Hormoz Mahmoudvand, Mojtaba Shakibaie, Majid Fasihi Harandi, Ebrahim Saedi Dezaki
Journal of Investigative Surgery.2016; 29(3): 137. CrossRef
Hidatidosis hepática: todavía un problema
Victoria Busto Bea, Jesús Barrio Andrés, Carolina Almohalla Álvarez
Medicina Clínica.2016; 146(8): 367. CrossRef
Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract
Farnaz Kheirandish, Bahram Delfan, Hossein Mahmoudvand, Nasim Moradi, Behrouz Ezatpour, Farzad Ebrahimzadeh, Marzieh Rashidipour
Biomedicine & Pharmacotherapy.2016; 82: 208. CrossRef
Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery
Hossein Mahmoudvand, Farnaz Kheirandish, Ebrahim Saedi Dezaki, Saeedeh Shamsaddini, Majid Fasihi Harandi
Biomedicine & Pharmacotherapy.2016; 82: 393. CrossRef
Liver hydatid disease: Still a problem
Victoria Busto Bea, Jesús Barrio Andrés, Carolina Almohalla Álvarez
Medicina Clínica (English Edition).2016; 146(8): 367. CrossRef
Efficacy and Safety ofBunium Persicum(Boiss) to Inactivate Protoscoleces during Hydatid Cyst Operations
Hossein Mahmoudvand, Razieh Tavakoli Oliaei, Seyed Reza Mirbadie, Farnaz Kheirandish, Amir Tavakoli Kareshk, Behrouz Ezatpour, Hormoz Mahmoudvand
Surgical Infections.2016; 17(6): 713. CrossRef
Leishmaniosis phytotherapy: Review of plants used in Iranian traditional medicine on leishmaniasis
Mahmoud Bahmani, Kourosh Saki, Behrouz Ezatpour, Somayeh Shahsavari, Zohreh Eftekhari, Mahyar Jelodari, Mahmoud Rafieian-Kopaei, Reza Sepahvand
Asian Pacific Journal of Tropical Biomedicine.2015; 5(9): 695. CrossRef
Phytochemical Compositions and Biological Activities of Essential Oil from Xanthium strumarium L.
Javad Sharifi-Rad, Seyedeh Hoseini-Alfatemi, Majid Sharifi-Rad, Mehdi Sharifi-Rad, Marcello Iriti, Marzieh Sharifi-Rad, Razieh Sharifi-Rad, Sara Raeisi
Molecules.2015; 20(4): 7034. CrossRef

14,504 View
183 Download
49 Web of Science
Crossref

Brief Communications

Prevalence of Hydatid Cysts in Livestock Animals in Xinjiang, China: Meng Qingling, Wang Guanglei, Qiao Jun, Zhu Xinquan, Liu Tianli, Song Xuemei, Zhang Jinsheng, Wang Huisheng, Cai Kuojun, Chen Chuangfu; Korean J Parasitol 2014;52(3):331-334.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.331

Abstract
PDF

Hydatid worms, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. To better understand the disease infection status in Xinjiang, China, we investigated the disease epidemics in 4 livestock animals, i.e., cattle, sheep (both sheep and goat), camels, and horses, slaughtered at the abattoirs in Urumqi, Yining, Tacheng, and Altay areas. The results showed that the animals were infected at different rates, in the order of sheep (9.8%), cattle (8.4%), camels (6.8%), and horses (4.3%). The infection rates were found to be different between the abattoirs in various regions even for the same animals. For sheep, the rates increased significantly as the animals grew older. It was 1.9% before 1 year of age and increased to 8.2% in the age of 1-2 years, and further increased to 12.3% when the animals were 3-4 years old, and reached 17.2% when they were 5-6 year old. Sheep older than 6 years had an infection rate of 19.5%. This study demonstrates that the 4 livestock animals in the pastoral areas in Xinjiang were infected by the parasites to various extend. This study is the first systematic investigation of the hydatid worms in various livestock animals in Xinjiang, China, which provides epidemiological information about the infection of hydatid worms in livestock, and is valuable in developing strategies for prevention and control of the hydatid disease.

Citations

Citations to this article as recorded by

Prevalence of Hydatidosis (Echinococcus granulosus) in Slaughtered Ruminants in and out of Abattoir in Duhok Area and its Correlation with Some Variables.
Rekesh S. Habib, Adnan M. Abdullah
Zanin Journal of Science and Engineering.2025; 1(2): 45. CrossRef
Investigating the Epidemiology and Socioecological Dynamics of Hydatid-like Cysts Within a Specific Endemic District
Muhammad Irfan Khan, Rahmat Ali, Dejun Ji, Wei Lan, Ahmed A. Saleh, Shahab Ur Rehman, Muhammad Shuaib, Shah Zeb Ahmad, Hussain, Xi Wu
Animals.2025; 15(11): 1617. CrossRef
Prevalence of cystic echinococcosis among livestock in pastoral and agro-pastoral areas in Uganda
Leonard Omadang, Martin Chamai, Francis Ejobi, Joseph Erume, Peter Oba, Michael Ocaido
Parasitology.2024; 151(1): 68. CrossRef
Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
Pathogens.2023; 12(1): 134. CrossRef
Effect and mechanism of reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis
Cai-Song Chen, Yao-Gang Zhang, Hai-Jiu Wang, Hai-Ning Fan
World Journal of Gastroenterology.2023; 29(14): 2153. CrossRef
Meta-analysis on the prevalence of bovine hydatid disease in China from 2000 to 2021
Shijie Fan, Hailong Dong, Hongcai Ma, Bingyi Wang, Mujahid Iqbal, Minghao Zou, Ming Qi, Zhipeng Cao
Microbial Pathogenesis.2022; 168: 105586. CrossRef
Cystic echinococcosis in slaughtered sheep in Erzurum province, Turkey
Hamza Avcioglu, Mohammed Mebarek Bia, Ibrahim Balkaya, Ridvan Kirman, Muzaffer Akyuz, Esin Guven
Acta Tropica.2022; 233: 106559. CrossRef
Oscillatory properties of class C notifiable infectious diseases in China from 2009 to 2021
Yanxiang Cao, Meijia Li, Naem Haihambo, Yuyao Zhu, Yimeng Zeng, Jianhua Jin, Jinyi Qiu, Zhirui Li, Jiaxin Liu, Jiayi Teng, Sixiao Li, Yanan Zhao, Xixi Zhao, Xuemei Wang, Yaqiong Li, Xiaoyang Feng, Chuanliang Han
Frontiers in Public Health.2022;[Epub] CrossRef
The occurrence of cystic echinococcosis in slaughtered livestock in Jahrom, south of Iran
Manoochehr Shabani, Kavous Solhjoo, Ali Taghipour, Abdolreza Sotoodeh Jahromi, Saina Karami, Belal Armand
Parasite Epidemiology and Control.2022; 19: e00274. CrossRef
Modelling Neurological Diseases in Large Animals: Criteria for Model Selection and Clinical Assessment
Samantha L. Eaton, Fraser Murdoch, Nina M. Rzechorzek, Gerard Thompson, Claudia Hartley, Benjamin Thomas Blacklock, Chris Proudfoot, Simon G. Lillico, Peter Tennant, Adrian Ritchie, James Nixon, Paul M. Brennan, Stefano Guido, Nadia L. Mitchell, David N.
Cells.2022; 11(17): 2641. CrossRef
Meta-analysis of the prevalence of bovine cystic echinococcosis in China during decade
Xin-Bo Yang, Xiang-Zhu Meng, Yan Zhao, Jin-Ping Zhao, Chao Chen, Ya Qin, Yuan Zhang, Quan Zhao
Research in Veterinary Science.2022; 152: 465. CrossRef
Current Situation of Cystic Echinococcosis in Sheep in Turkey
Hamza Avcioglu, Mohammed Mebarek Bia, Ibrahim Balkaya, Ridvan Kirman, Muzaffer Akyuz, Esin Güven
SSRN Electronic Journal .2022;[Epub] CrossRef
Prevalence and bacterial isolation from hydatid cysts in dromedary camels (Camelus dromedarius) slaughtered at Sharkia abattoirs, Egypt
Asmaa Basiony Ahmed, Refaat Ras, Abdallah F. Mahmoud, Emad El-Ghazaly, Giovanni Widmer, Hesham Dahshan, Ibrahim Elsohaby
Journal of Parasitic Diseases.2021; 45(1): 236. CrossRef
The global status and genetic characterization of hydatidosis in camels (Camelus dromedarius): a systematic literature review with meta-analysis based on published papers
Davood Anvari, Nima Pourmalek, Saeed Rezaei, Amir Fotovati, Seyed Abdollah Hosseini, Ahmad Daryani, Adel Spotin, Shahabeddin Sarvi, Mona Hosseini, Mohammad Reza Narouei, Mohammad Kalkali, Faezeh Pendar, Shirzad Gholami
Parasitology.2021; 148(3): 259. CrossRef
Advances in research on echinococcoses epidemiology in China
Mei-Hua Fu, Xu Wang, Shuai Han, Ya-Yi Guan, Robert Bergquist, Wei-Ping Wu
Acta Tropica.2021; 219: 105921. CrossRef
Prevalence of cystic echinococcosis in slaughtered livestock in Iran: a systematic review and meta-analysis
Aliakbar Vaisi-Raygani, Masoud Mohammadi, Rostam Jalali, Nader Salari, Melika Hosseinian-Far
BMC Infectious Diseases.2021;[Epub] CrossRef
Prevalence and Molecular Characterization of Cystic Echinococcosis in Livestock Population of the Malakand Division, Khyber Pakhtunkhwa, Pakistan
Jadoon Khan, Nosheen Basharat, Salman Khan, Syed Muhammad Jamal, Sadeeq ur Rahman, Aamer Ali Shah, Sanaullah Khan, Rehman Ali, Shahid Niaz Khan, Ijaz Ali
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Prevalence and financial loss estimation of parasitic diseases detected in slaughtered cattle in Kwara State, North-central Nigeria
Shola David Ola-Fadunsin, Patricia Isioma Uwabujo, Ibrahim Ndaman Halleed, Bukola Richards
Journal of Parasitic Diseases.2020; 44(1): 1. CrossRef
Analysis of Factors Influencing Cystic Echinococcosis in Northwest Non-Qinghai Tibetan Plateau Regions of China
Xiangman Zeng, Yayi Guan, Weiping Wu, Liying Wang, Huixia Cai, Qi Fang, Shicheng Yu, Canjun Zheng
The American Journal of Tropical Medicine and Hygiene.2020; 102(3): 567. CrossRef
Seroprevalence of bovine echinococcusis in Pakistan
Fariha Aziz, Zahida Tasawar, Mushtaq Hussain Lashari
Ciência Rural.2020;[Epub] CrossRef
In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan
Mughees Aizaz Alvi, John Asekhaen Ohiolei, Li Li, Muhammad Saqib, Muhammad Hammad Hussain, Muhammad Haleem Tayyab, Muzafar Ghafoor, Warda Qamar, Noman Yousaf Faridi, Anum Aizaz Alvi, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Pathogens.2020; 9(11): 905. CrossRef
Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
Baoping Guo, Zhuangzhi Zhang, Xueting Zheng, Yongzhong Guo, Gang Guo, Li Zhao, Ren Cai, Bingjie Wang, Mei Yang, Xi Shou, Wenbao Zhang, Bin Jia
The Korean Journal of Parasitology.2019; 57(2): 153. CrossRef
Epidemiology of taeniosis, cysticercosis and trichinellosis in Iran: A systematic review
Mohammad Moazeni, Faham Khamesipour, Douglas N. Anyona, Gabriel O. Dida
Zoonoses and Public Health.2019; 66(1): 140. CrossRef
Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
Liu Tianli, Wang Xifeng, Tian Zhenzhong, Wang Lixia, Zhang Xingxing, Qiao Jun, Meng Qingling, Gong Shasha, Chen Ying, Cai Xuepeng
The Korean Journal of Parasitology.2019; 57(1): 61. CrossRef
Prevalence and monetary loss due to cystic Echinococcosis in slaughter house livestock: A case study of Migori County, Kenya
Odero Joseph Kere, Erume Joseph, Banda Liveness Jessika, Kagira John Maina
Parasite Epidemiology and Control.2019; 5: e00105. CrossRef
The current prevalence and diversity of cystic echinococcosis in slaughtered animals in Egypt
Khaled Mohamed El-Dakhly, Waleed M. Arafa, El-Shaymaa N. El-Nahass, Khaled A. M. Shokier, Asmaa F. Noaman
Journal of Parasitic Diseases.2019; 43(4): 711. CrossRef
Prevalence of liver flukes infections and hydatidosis in slaughtered sheep and goats in Nishapour, Khorasan Razavi, Iran
Majid Aminzare, Mohammad Hashemi, Samaneh Yaghoobi Faz, Mojtaba Raeisi, Hassan Hassanzadazar
Veterinary World.2018; 11(2): 146. CrossRef
Novel Interventional Management of Hepatic Hydatid Cyst with Nanosecond Pulses on Experimental Mouse Model
Xinhua Chen, Ruiqing Zhang, Tuerganaili Aji, Yingmei Shao, Yonggang Chen, Hao Wen
Scientific Reports.2017;[Epub] CrossRef
Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction
Rui-Qing Zhang, Xin-Hua Chen, Hao Wen
World Journal of Gastroenterology.2017; 23(45): 7989. CrossRef
First serological study of equine hydatidosis in Iran
Ehsanollah Sakhaee, Mehdi Golchin, Hamidreza Amiri, Mohammad Reza Fayed, Jamaladdin Eydi
Journal of Parasitic Diseases.2016; 40(4): 1567. CrossRef
Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran
M.T. Rahimi, S. Sarvi, A. Daryani, M. Sharif, E. Ahmadpour, A. Shokri, A. Mizani
Helminthologia.2016; 53(3): 285. CrossRef

9,556 View
117 Download
26 Web of Science
Crossref

Effects of Different Sizes of Glass Beads on the Release of Sporocysts from Eimeria tenella Oocysts: Myung-Jo You; Korean J Parasitol 2014;52(3):317-319.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.317

Abstract
PDF

The oocyst wall is severed by means of mechanical injury or chemical agents. This study reports the percentage of in vitro sporocyst release following mechanical shaking in the presence of varying sizes of glass beads. Glass beads measured 0.5, 1, and 3 mm in diameter and were shaken with the oocysts for different times ranging from 5 sec to 5 min. Approximately 80% of sporocysts were released with 5 min of shaking in the presence of 3 mm glass beads, as well as 30 sec with 0.5 mm beads and 1 mm glass beads. The release of sporocysts of E. tenella was most efficient using 1 mm glass beads and treatment times of 30 sec to 1 min. Therefore, the use of 1 mm glass beads with 30 sec to 1 min of agitation is recommended in order to maximize sporocyst release and recovery and to improve the yield of viable sporozoites for use in biochemical, tissue culture, and immunological applications of coccidia.

Citations

Citations to this article as recorded by

An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts
Aruto Takano, Dennis V. Umali, April H. Wardhana, Dyah H. Sawitri, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi
Poultry Science.2025; 104(3): 104810. CrossRef
Multicenter comparative study of Enterocytozoon bieneusi DNA extraction methods from stool samples, and mechanical pretreatment protocols evaluation
Céline Nourrisson, Maxime Moniot, Maxime Tressol, Céline Lambert, Emilie Fréalle, Florence Robert-Gangneux, Damien Costa, Louise Basmaciyan, Philippe Poirier
Scientific Reports.2024;[Epub] CrossRef
Anti-Eimeria tenella activity of Ethanamizuril in vitro and in vivo
Hongbo CHEN, Yindi DAI, Jiyu LIU, Lianmao DUAN, Juan HU, Shaohui SUN, Guangshan ZHU, Xiaoping MA, Jin WAN, Nini MA, Xiao ZHANG, Cong WANG, Ningning ZHAO
Journal of Veterinary Medical Science.2024; 86(9): 1008. CrossRef
Comparative Study of Eleven Mechanical Pretreatment Protocols for Cryptosporidium parvum DNA Extraction from Stool Samples
Laure Claudel, Nicolas Valeix, Louise Basmaciyan, Bruno Pereira, Damien Costa, Anne Vincent, Stéphane Valot, Loic Favennec, Frederic Dalle
Microorganisms.2021; 9(2): 297. CrossRef
Statistical Prediction of the Peak Point (Time) Required for Release of Maximum Number of Sporocysts after Eimeria Tenella Oocyst Excystation
JO Cha, KS Shim, HW Lee, HC Kim
Brazilian Journal of Poultry Science.2021;[Epub] CrossRef
Effect of different floatation solutions on E. tenella oocyst purification and optimization of centrifugation conditions for improved recovery of oocysts and sporocysts
Nanshan Qi, Shenquan Liao, Asmaa M.I. Abuzeid, Juan Li, Caiyan Wu, Minna Lv, Xuhui Lin, Junjing Hu, Wenwan Xiao, Mingfei Sun, Guoqing Li
Experimental Parasitology.2020; 217: 107965. CrossRef
Multicenter Comparative Study of Six Cryptosporidium parvum DNA Extraction Protocols Including Mechanical Pretreatment from Stool Samples
Nicolas Valeix, Damien Costa, Louise Basmaciyan, Stéphane Valot, Anne Vincent, Romy Razakandrainibe, Florence Robert-Gangneux, Céline Nourrisson, Bruno Pereira, Emilie Fréalle, Philippe Poirier, Loic Favennec, Frederic Dalle
Microorganisms.2020; 8(9): 1450. CrossRef
The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
Nanshan Qi, Shenquan Liao, Asmaa M. I. Abuzeid, Juan Li, Caiyan Wu, Minna Lv, Xuhui Lin, Junjing Hu, Linzeng Yu, Wenwan Xiao, Mingfei Sun, Guoqing Li
Scientific Reports.2019;[Epub] CrossRef
Molecular and biochemical characterization of Eimeria tenella hexokinase
Mingfei Sun, Shenquan Liao, Longxian Zhang, Caiyan Wu, Nanshan Qi, Minna Lv, Juan Li, Xuhui Lin, Jianfei Zhang, Mingquan Xie, Guan Zhu, Jianping Cai
Parasitology Research.2016; 115(9): 3425. CrossRef
Effect of in vitro and in vivo 25-hydroxyvitamin D treatment on macrophages, T cells, and layer chickens during a coccidia challenge12
A. Morris, R. Shanmugasundaram, J. McDonald, R. K. Selvaraj
Journal of Animal Science.2015; 93(6): 2894. CrossRef

10,945 View
154 Download
10 Web of Science
Crossref

Recent Situation of Taeniasis in Mongolia (2002-2012): Anu Davaasuren, Temuulen Dorjsuren, Tetsuya Yanagida, Yasuhito Sako, Kazuhiro Nakaya, Abmed Davaajav, Gurbadam Agvaandaram, Tsatsral Enkhbat, Battsetseg Gonchigoo, Nyamkhuu Dulmaa, Gantigmaa Chuluunbaatar, Akira Ito; Korean J Parasitol 2014;52(2):211-214.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.211

Abstract
PDF

Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia species and copro-PCR and loop-mediated isothermal amplification (LAMP) assays were utilized for detection of copro-DNA of 37 fecal samples from taeniasis patients submitted to the Mongolian National Center for Communicable Diseases (NCCD) from 2002 to 2012. In addition, 4 out of 44 calcified cysts in beef kept in formalin since 2003 were evaluated for histopathological confirmation of cattle cysticercosis. All proglottid specimens and stool samples were confirmed to be Taenia saginata by multiplex PCR and by copro-PCR and LAMP, respectively. Cysts collected from cattle were morphologically confirmed to be metacestodes of Taenia species. T. saginata taeniasis was identified from almost all ages from a 2-year-old boy up to a 88-year-old woman and most prominently in 15-29 age group (37%, 74/198) followed by 30-44 age group (34.8%, 69/198 ) from 15 of Mongolia's 21 provinces, while cattle cysticerci were found from 12 provinces. The highest proportion of taeniasis patients was in Ulaanbaatar, the capital of Mongolia.

Citations

Citations to this article as recorded by

Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia
Sayed Ajmal Qurishi, Hong-Bin Yan, Li Li, John Aeskhaen Ohiolei, Mughees Aizaz Alvi, Lin-Sheng Zhang, Ha Da, Hong-Mei Qiao, Nigus Abebe Shumuye, Bao Hua, Bing-Xin Bai, Wen-Jun Tian, Ju-Mei Xu, Bao-Quan Fu, Wan-Zhong Jia
Parasitology Research.2022; 121(12): 3455. CrossRef
Prevalence of meat-transmitted Taenia and Trichinella parasites in the Far East countries
Yi Liu, Zijian Dong, Jianda Pang, Mingyuan Liu, Xuemin Jin
Parasitology Research.2021; 120(12): 4145. CrossRef
Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny
Parasites & Vectors.2020;[Epub] CrossRef
Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook
Miao-Han Deng, Lan-Yi Zhong, Okanurak Kamolnetr, Yanin Limpanont, Zhi-Yue Lv
Infectious Diseases of Poverty.2019;[Epub] CrossRef
Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
Acta Tropica.2019; 198: 105075. CrossRef
Neurocysticercosis: A case study of a Mongolian traveler who visited China and India with an updated review in Asia
Anu Davaasuren, Abmed Davaajav, Baigalmaa Ukhnaa, Altantsetseg Purvee, Saraa Unurkhaan, Amartuvshin Luvsan, Jenae E. Logan, Akira Ito
Travel Medicine and Infectious Disease.2017; 20: 31. CrossRef
Novel approaches to the diagnosis of Strongyloides stercoralis infection
D. Buonfrate, F. Formenti, F. Perandin, Z. Bisoffi
Clinical Microbiology and Infection.2015; 21(6): 543. CrossRef

9,636 View
82 Download
8 Web of Science
Crossref

Original Article

Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2014;52(2):131-135.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.131

Abstract
PDF

Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii
Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio
Nature Protocols.2024; 19(1): 3. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Suthinee Sangkanu, Abolghasem Siyadatpanah, Roghayeh Norouzi, Julalak Chuprom, Watcharapong Mitsuwan, Sirirat Surinkaew, Rachasak Boonhok, Alok K. Paul, Tooba Mahboob, Imran Sama-ae, Sonia M. R. Oliveira, Tajudeen O. Jimoh, Maria de Lourdes Pereira, Polra
PeerJ.2024; 12: e16937. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Evolution of giant pandoravirus revealed by CRISPR/Cas9
Hugo Bisio, Matthieu Legendre, Claire Giry, Nadege Philippe, Jean-Marie Alempic, Sandra Jeudy, Chantal Abergel
Nature Communications.2023;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens
Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Wilawan Mahabusarakam, L. Ravithej Singh, Ekachai Dumkliang, Kritamorn Jitrangsri, Alok K. Paul, Sirirat Surinkaew, Polrat Wilairatana, Maria de Lourdes Pereira, Mohammed Rahmatu
PeerJ.2022; 10: e14468. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage
Ruixuan Zhang, Hisashi Endo, Masaharu Takemura, Hiroyuki Ogata, Samuel K. Campos
Microbiology Spectrum.2021;[Epub] CrossRef
Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector
Christopher Price, Snake Jones, Mirna Mihelcic, Marina Santic, Yousef Abu Kwaik
Cell Host & Microbe.2020; 27(4): 571. CrossRef
Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene in Acanthamoeba castellanii
Steven Rolland, Luce Mengue, Cyril Noël, Stéphanie Crapart, Anne Mercier, Willy Aucher, Yann Héchard, Ascel Samba-Louaka
Pathogens.2020; 9(5): 321. CrossRef
The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
Parasites & Vectors.2020;[Epub] CrossRef
Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture
Mária Garajová, Martin Mrva, Naděžda Vaškovicová, Michal Martinka, Janka Melicherová, Andrea Valigurová
Scientific Reports.2019;[Epub] CrossRef
The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls
Pamela Magistrado-Coxen, Yousuf Aqeel, Angelo Lopez, John R. Haserick, Breeanna R. Urbanowicz, Catherine E. Costello, John Samuelson, Photini Sinnis
PLOS Neglected Tropical Diseases.2019; 13(5): e0007352. CrossRef
A review of Acanthamoeba keratitis in the middle East and Iran
MohammadHossein Feiz Haddad, Azar Shokri, Habib Habibpour, SeyedeManizhe Heidar Neiadi
Journal of Acute Disease.2019; 8(4): 133. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii
Duo Wu, Meng Feng, Zhi-xin Wang, Ke Qiao, Hiroshi Tachibana, Xun-jia Cheng
Parasites & Vectors.2018;[Epub] CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef
In-vitro development of an effective treatment for Acanthamoeba keratitis
Á. Ortillés, J. Belloc, E. Rubio, M.T. Fernández, M. Benito, J.Á. Cristóbal, B. Calvo, P. Goñi
International Journal of Antimicrobial Agents.2017; 50(3): 325. CrossRef
Treatment of Acanthamoeba keratitis
N. R. Marchenko, Evg. A. Kasparova
Vestnik oftal'mologii.2016; 132(5): 110. CrossRef
Cellulose degradation: a therapeutic strategy in the improved treatment of Acanthamoeba infections
Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Parasites & Vectors.2015;[Epub] CrossRef
An update onAcanthamoebakeratitis: diagnosis, pathogenesis and treatment
Jacob Lorenzo-Morales, Naveed A. Khan, Julia Walochnik
Parasite.2015; 22: 10. CrossRef
Potential Value of Cellulose Synthesis Inhibitors Combined With PHMB in the Treatment of Acanthamoeba Keratitis
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Cornea.2015; 34(12): 1593. CrossRef